pycom16g16960

Ethylene-responsive protein kinase Le-CTR1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0007173
Physical Location & Seq
Forward (+)
12953728 .. 12960166
6439 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 3366 bp
ATGCAAGTCCCCAATTCTGACGAAGCTGCCCCCGAAAGCACTAAGGTACCTGAGCAATCAGAAGCTGAAACCTCCAATCCGACTGTCGAAGATGAAGACGGCGGCGCCGCGGCCGACGGTGACGACGACCCTAGGGTTGTCGACATATCCGGCAAAAGCTTGGACTTTTCGATTGGGGAGAATTCTGAGGATGTCGGGGCTTTGTATTTGTACAAAAACGTGTTTAATTTATTGCCCAAGTCGATTGGCTCTCACAAGCGGTTGAGGACGCTGAAGTTCTTTGGGAATGAGATTAATTTATTTTCGCCTTCAGCGGCAATGGAGTTTGAGAGCTTGGAATGCTTGCAAGTGAGGATGTCGTCGCCGGAGTTTTGTGGGTTGCCGTTGGATAAATTGAGTGGCTTGAAGGAGCTTGAGCTTTCCAAAGTGCCGACGAGGCCTTCGGGGTTTCAGATATTGAGCGAGATTGCTCGGCTCAAGTGCCTCACCAAGCTCTCTGTTTGTCACTTCTCCATAAGATACCTCCCTCCAGAAATTGGCTGCTTAAACACTTTGGAATATCTAGATCTTTCATTCAACAAGATGAAGAATTTGCCTGCTGAAATCAGTAATTTAAATGACTTGATATCGTTAAAAGTTGCTAATAATAAATTGGTGGAATTACCTTCGACTTTGTCCTCTTTGCAAAGGTTGGAGATCATGGACCTGTCAAATAATAGGTTGACATCACTAGGGTCTCTTGAACTTGACTTGATGCACAACCTTCAGATTTTAAATCTTCAGTACAACAAGCTTCTCACTTTCTGTCAAATACCCTCATGGATATGTTGCAATTTGGAAGGAAGTGGGAAGGACACACACAGTGATGATATTTCCATTTCCTCTGTTGAAATGGATGTATATGAAACCCCCCTTCAGAAAAATAATGAAAGCCATTTTCGTAGAGGCTTTCATCATAACTCAGCAAGTCTAATTTTCACGCATTCATCTACCAGTAGGTGCTGTGCAACTCGGAGGTCAGGTAGGTGGAGGAAGCAAGGGTATAGGTTGCAGCAGAGAGCTCGTCAAGAACGCTTAAACAACAGCAGGAAGTTGAAAGGTCTTGATCTTTCCAAGCAGTTACATGTGAAGGAAGATGGAGAATGCAAACCAGGCAACCCTGACCTTCTTGCTTCTGAATCTTACCCTGAAGGTGCATCAGTCATTATCAATCTGGACGATGATGTTGAGAAAGATTTACCATCTAGGGAAGTGCAAAGTGAAAATGTTGTGTGCCATGATATGAGTTTGAAGGAGCATTCTGTAGGAAATTCCTCATCAGTGAGTCCAGACTCTAGTGCAGTAGATGAAAGTGATGAAAAGGATTGTTGTGAGTTTGATGCATCGTCGGCCCCCAATCAACGGGTTTATGGTGAGGAAGATGAAGGTTCATCTTCAGATACATCAAAAAGTATTTGTCAACCCAAAAGGCACCCTGATGGGTGCCTTGATAATCCGAGACAGCCCAAACATCCTAGATACAGTTCTAATAGTTCAAACTTGTCTCGGAAATATAATGACATCTCAGTTTGCAGCACTGAGGACCATCTTTCAGATGGCTTTTACGATGCAGGGCGTGACAGGCCATTCATGCCACTAGAACTCTATGATGAAAAGTTCTGTCTTGACTCACGTGAAGTCATTCTCTTGGACAGGCAATGGGATAAAGAGTTGGATGTAATTTTATGTTCTGCCCAAGAATTGGTGAATCGTTTAAATACGGATGGAAATAAGGCTGATATGCTGCAGATTGCATCACTACTTGCTCTGTTTGTATCAGATCACTTTGGGGGGAGTGATAGGAGTGCTCTTGTTGAATGGTCACGAAAAGCTAATCCTCTTTCAGACTACAAGAAGCCTTTTGTTTGCACCTGCCCAACTGGAAACAAGGGCTTTATTAGTTTGTCCACTAAACCAGTTGTAAAGAATGTTGAAGATATCATTTTTTCTGATGTCTGTGAGAAATCTCTTCATTCTATCAAAGCACGACGAAATTCCATTGTAGTTCCCATTGGAACCTTGCAGTTTGGTGTGTGTCGGCATAGAGCATTGCTATTGAAGTATCTTTGTGATTGGGTGGAGCCTCGAGTACCTTGTGAGCTGATTAGGGGTTATTTGGATTTCATGCCACATGCGTGGAATATTGTATTAATCAAGAGAGGTGCCAAAGAGATTCGAATGTTAGTTGATGCATGTCGTCCACATGATATAAGAGCGGAGACGGATCCCGAATATTATTGTAGGTATATTCCTCTCAGTCGGACCAAAGTTTATCTTCCAATTCGAAACAGCCCTGCTCCTACCTCCTTACATTCTATGCCCTCTTGTGATGAAACTCTGAAAAACTCTGTTACTTCTCTCGTCCGGCGCAAATATGGATCGATCATAAATGAGGCAGCAGGAAAGATGCGTACTTCTGTGGTATATGGAACTTCAACGGATGAAATTAGGAACTTTGACTACAACTGCTTAGGGGAAATAAGGATTTTAGGTGCTTTGAAACACCCTTGCATAGTGGAAATGTATGGACATCAGATATCTTCCAAGTGGGTTCCTCCTGGAGATGGTAATAGTGAGCATCGTATATTGCAGTCTATAATTTGGATGGAGGACATAAAAGGGGGTTCCTTACAGAATTATATAGAAAAGTTATCAAAAGCTGGTGAGAAGCATCTCCCAGTGGAGTTAGCTTTGCGCATTGCTAAAAATGTTGCATGTGCACTGGTGGAGCTGCATTCAAAGCACATTATTCACCGGGACATAAAAAGTGCAAACATATTGATTGATCTGGATAGAAAGAGAGCTGATGGAACACCTGTTGTGAAGCTCTGTGATTTTGATAGGGCAATTCCCCTTCGATCTTACTTGCATACGTGCTGCATTGCCCACGTTGGAATACCTTCTGCTAATGTTTGTGCTGGAACACCTCGCTGGATGGCCCCAGAGGTTTTGCGAGCAGTGCATGAACAAAATATTTATGGATTGGAAATTGATATCTGGTCATTTGGGTGCTTGCTGTTGGAAATGTTGACTTTGCAAATTCCGTACTTAGGAGTACCTGACTTGGAGATTCATGAACTTCTGACGAAGGGAAAACGACCAAATTTAACGGATGACTTGGAGGTGTTTCGGTCGTTGAATGAGCCAGTAATGACTCAAGCGGGCGCAGAACTTGATGGAACAGAGGCTGACTTAGACACACTGAGATTTCTTGTTGACTTGTTTTACCAATGTACGGAGGAGAATCCGGAGAATCGTCCTACAGCCGACAACCTCTACGAATTGATACTGGAGCACAGCAAAACTCATACAAACTCCGAAACTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EDR1 LRR_8 Pkinase

Protein Analysis

1122

Amino Acids

125.87

Weight (kDa)

5.54

Isoelectric Point (pI)

48.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_14 PF23598 153 - 263 2.4e-10 Leucine-rich repeat region
EDR1_CTR1_ARMC3_pept PF14381 665 - 743 3.5e-12 EDR1/CTR1/ARMC3, peptidase-like
Pkinase PF00069 834 - 1106 1.2e-33 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 834 - 1049 2.3e-23 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1919
Acc16I TGCGCA 1 cut(s) 2735
Acc36I ACCTGC 1 cut(s) 1919
Acc65I GGTACC 1 cut(s) 46
AccB1I GGYRCC 5 cut(s) 46, 104, 1470, 1482, 2201
AccB7I CCANNNNNTGG 2 cut(s) 536, 1741
AccBSI CCGCTC 1 cut(s) 2255
AccI GTMKAC 1 cut(s) 141
AccII CGCG 1 cut(s) 110
AccIII TCCGGA 1 cut(s) 3286
AciI CCGC 7 cut(s) 102, 108, 110, 259, 314, 2255, 3200
AclWI GGATC 3 cut(s) 2258, 2271, 2425
AcoI YGGCCR 1 cut(s) 111
AcsI RAATTY 6 cut(s) 181, 589, 1309, 2032, 3078, 3142
AcuI CTGAAG 7 cut(s) 293, 294, 749, 764, 899, 1209, 1419
AcvI CACGTG 1 cut(s) 1673
AcyI GRCGYC 1 cut(s) 105
AdeI CACNNNGTG 1 cut(s) 863
AfaI GTAC 8 cut(s) 48, 212, 785, 2130, 2452, 3086, 3096, 3274
AfiI CCNNNNNNNGG 6 cut(s) 536, 1401, 1741, 2603, 3089, 3274
AflIII ACRYGT 2 cut(s) 219, 1123
AjnI CCWGG 2 cut(s) 1150, 2596
AleI CACNNNNGTG 1 cut(s) 2173
Alw21I GWGCWC 4 cut(s) 1063, 1849, 2761, 3336
Alw26I GTCTC 4 cut(s) 741, 1492, 1548, 2252
Alw44I GTGCAC 1 cut(s) 2757
AlwI GGATC 3 cut(s) 2258, 2271, 2425
AlwNI CAGNNNCTG 2 cut(s) 65, 3227
Ama87I CYCGRG 1 cut(s) 2124
Aor13HI TCCGGA 1 cut(s) 3286
AoxI GGCC 5 cut(s) 111, 437, 1389, 1622, 2976
ApaLI GTGCAC 1 cut(s) 2757
ApeKI GCWGC 8 cut(s) 26, 540, 1051, 1572, 1783, 2435, 2770, 2916
ApoI RAATTY 6 cut(s) 181, 589, 1309, 2032, 3078, 3142
ArsI GACNNNNNNTTYG 2 cut(s) 424, 456
AseI ATTAAT 2 cut(s) 294, 2189
Asp700I GAANNNNTTC 2 cut(s) 1680, 2938
Asp718I GGTACC 1 cut(s) 46
AspA2I CCTAGG 1 cut(s) 131
AspLEI GCGC 4 cut(s) 107, 2409, 2736, 3206
AspS9I GGNCC 5 cut(s) 703, 1390, 1582, 2301, 2977
AsuC2I CCSGG 1 cut(s) 2795
AsuHPI GGTGA 6 cut(s) 131, 478, 1424, 1756, 2714, 2783
AsuII TTCGAA 2 cut(s) 2215, 2323
AvaI CYCGRG 1 cut(s) 2124
AvaII GGWCC 3 cut(s) 703, 1582, 2301
AvrII CCTAGG 1 cut(s) 131
BaeGI GKGCMC 1 cut(s) 2761
BaeI ACNNNNGTAYC 2 cut(s) 30, 63
BamHI GGATCC 1 cut(s) 2263
BanI GGYRCC 5 cut(s) 46, 104, 1470, 1482, 2201
BanII GRGCYC 1 cut(s) 1063
BbrPI CACGTG 1 cut(s) 1673
BbsI GAAGAC 1 cut(s) 102
Bbv12I GWGCWC 4 cut(s) 1063, 1849, 2761, 3336
BbvI GCAGC 8 cut(s) 13, 527, 1063, 1584, 1770, 2447, 2757, 2903
BceAI ACGGC 2 cut(s) 115, 367
BcgI CGANNNNNNTGC 2 cut(s) 1043, 1077
BciT130I CCWGG 2 cut(s) 1152, 2598
BcnI CCSGG 1 cut(s) 2795
BcoDI GTCTC 4 cut(s) 741, 1492, 1548, 2252
BfaI CTAG 7 cut(s) 132, 563, 731, 1245, 1335, 1515, 1637
BfmI CTRYAG 3 cut(s) 1302, 1784, 3300
BfoI RGCGCY 1 cut(s) 108
BfuAI ACCTGC 1 cut(s) 1919
BglI GCCNNNNNGGC 1 cut(s) 436
BglII AGATCT 1 cut(s) 565
BlnI CCTAGG 1 cut(s) 131
Bme1390I CCNGG 3 cut(s) 1152, 2598, 2795
Bme18I GGWCC 3 cut(s) 703, 1582, 2301
BmeT110I CYCGRG 1 cut(s) 2124
BmgT120I GGNCC 5 cut(s) 703, 1390, 1582, 2301, 2977
BmrFI CCNGG 3 cut(s) 1152, 2598, 2795
BmrI ACTGGG 1 cut(s) 2711
BmuI ACTGGG 1 cut(s) 2711
BpiI GAAGAC 1 cut(s) 102
BpmI CTGGAG 3 cut(s) 513, 2619, 3350
Bpu10I CCTNAGC 2 cut(s) 51, 2509
Bpu14I TTCGAA 2 cut(s) 2215, 2323
BpuEI CTTGAG 3 cut(s) 434, 461, 3180
BpuMI CCSGG 1 cut(s) 2795
Bsa29I ATCGAT 1 cut(s) 2420
BsaAI YACGTR 2 cut(s) 1673, 2913
BsaHI GRCGYC 1 cut(s) 105
BsaI GGTCTC 1 cut(s) 741
BsaJI CCNNGG 2 cut(s) 108, 131
BsaWI WCCGGW 1 cut(s) 3286
BsaXI ACNNNNNCTCC 2 cut(s) 1006, 1036
Bsc4I CCNNNNNNNGG 6 cut(s) 536, 1401, 1741, 2603, 3089, 3274
Bse1I ACTGG 7 cut(s) 993, 1924, 1955, 2717, 2766, 3185, 3333
Bse3DI GCAATG 5 cut(s) 324, 1703, 2087, 2736, 2919
BseAI TCCGGA 1 cut(s) 3286
BseBI CCWGG 2 cut(s) 1152, 2598
BseCI ATCGAT 1 cut(s) 2420
BseDI CCNNGG 2 cut(s) 108, 131
BseLI CCNNNNNNNGG 6 cut(s) 536, 1401, 1741, 2603, 3089, 3274
BseMI GCAATG 5 cut(s) 324, 1703, 2087, 2736, 2919
BseMII CTCAG 7 cut(s) 42, 177, 975, 1569, 1578, 2308, 3233
BseNI ACTGG 7 cut(s) 993, 1924, 1955, 2717, 2766, 3185, 3333
BseRI GAGGAG 1 cut(s) 3293
BseSI GKGCMC 1 cut(s) 2761
BseX3I CGGCCG 1 cut(s) 111
BseXI GCAGC 8 cut(s) 13, 527, 1063, 1584, 1770, 2447, 2757, 2903
BsgI GTGCAG 1 cut(s) 1359
Bsh1236I CGCG 1 cut(s) 110
Bsh1285I CGRYCG 2 cut(s) 114, 3173
BshFI GGCC 5 cut(s) 113, 439, 1391, 1624, 2978
BshNI GGYRCC 5 cut(s) 46, 104, 1470, 1482, 2201
BshVI ATCGAT 1 cut(s) 2420
BsiEI CGRYCG 2 cut(s) 114, 3173
BsiHKAI GWGCWC 4 cut(s) 1063, 1849, 2761, 3336
BsiHKCI CYCGRG 1 cut(s) 2124
BsiSI CCGG 5 cut(s) 150, 365, 2404, 2794, 3287
BslFI GGGAC 1 cut(s) 2810
BslI CCNNNNNNNGG 6 cut(s) 536, 1401, 1741, 2603, 3089, 3274
BsmAI GTCTC 4 cut(s) 741, 1492, 1548, 2252
BsmBI CGTCTC 1 cut(s) 2252
BsmFI GGGAC 1 cut(s) 2810
BsmI GAATGC 5 cut(s) 344, 982, 1148, 1297, 2773
BsnI GGCC 5 cut(s) 113, 439, 1391, 1624, 2978
Bso31I GGTCTC 1 cut(s) 741
BsoBI CYCGRG 1 cut(s) 2124
Bsp119I TTCGAA 2 cut(s) 2215, 2323
Bsp1286I GDGCHC 4 cut(s) 1063, 1849, 2761, 3336
Bsp13I TCCGGA 1 cut(s) 3286
Bsp1407I TGTACA 1 cut(s) 210
Bsp143I GATC 9 cut(s) 565, 696, 1105, 1819, 2263, 2417, 2421, 2824, 2897
BspACI CCGC 7 cut(s) 102, 108, 110, 259, 314, 2255, 3200
BspANI GGCC 5 cut(s) 113, 439, 1391, 1624, 2978
BspCNI CTCAG 7 cut(s) 43, 178, 974, 1570, 1577, 2307, 3234
BspDI ATCGAT 1 cut(s) 2420
BspEI TCCGGA 1 cut(s) 3286
BspFNI CGCG 1 cut(s) 110
BspHI TCATGA 1 cut(s) 3112
BspMAI CTGCAG 1 cut(s) 1788
BspMI ACCTGC 1 cut(s) 1919
BspPI GGATC 3 cut(s) 2258, 2271, 2425
BspT104I TTCGAA 2 cut(s) 2215, 2323
BspT107I GGYRCC 5 cut(s) 46, 104, 1470, 1482, 2201
BspTNI GGTCTC 1 cut(s) 741
BsrBI CCGCTC 1 cut(s) 2255
BsrDI GCAATG 5 cut(s) 324, 1703, 2087, 2736, 2919
BsrGI TGTACA 1 cut(s) 210
BsrI ACTGG 7 cut(s) 993, 1924, 1955, 2717, 2766, 3185, 3333
BssECI CCNNGG 2 cut(s) 108, 131
BssMI GATC 9 cut(s) 565, 696, 1105, 1819, 2263, 2417, 2421, 2824, 2897
BssNI GRCGYC 1 cut(s) 105
BssT1I CCWWGG 1 cut(s) 131
Bst2UI CCWGG 2 cut(s) 1152, 2598
Bst4CI ACNGT 4 cut(s) 85, 119, 863, 1523
Bst6I CTCTTC 1 cut(s) 2013
BstACI GRCGYC 1 cut(s) 105
BstAUI TGTACA 1 cut(s) 210
BstBAI YACGTR 2 cut(s) 1673, 2913
BstBI TTCGAA 2 cut(s) 2215, 2323
BstC8I GCNNGC 5 cut(s) 344, 597, 2994, 3053, 3202
BstDSI CCRYGG 1 cut(s) 108
BstFNI CGCG 1 cut(s) 110
BstH2I RGCGCY 1 cut(s) 108
BstHHI GCGC 4 cut(s) 107, 2409, 2736, 3206
BstKTI GATC 9 cut(s) 568, 699, 1108, 1822, 2266, 2420, 2424, 2827, 2900
BstMAI GTCTC 4 cut(s) 741, 1492, 1548, 2252
BstMBI GATC 9 cut(s) 565, 696, 1105, 1819, 2263, 2417, 2421, 2824, 2897
BstMCI CGRYCG 2 cut(s) 114, 3173
BstMWI GCNNNNNNNGC 8 cut(s) 339, 436, 1152, 1388, 1621, 1630, 2779, 2998
BstNI CCWGG 2 cut(s) 1152, 2598
BstNSI RCATGY 4 cut(s) 1127, 2174, 2235, 2757
BstSCI CCNGG 3 cut(s) 1150, 2596, 2793
BstSFI CTRYAG 3 cut(s) 1302, 1784, 3300
BstSLI GKGCMC 1 cut(s) 2761
BstUI CGCG 1 cut(s) 110
BstV1I GCAGC 8 cut(s) 13, 527, 1063, 1584, 1770, 2447, 2757, 2903
BstV2I GAAGAC 1 cut(s) 102
BstX2I RGATCY 2 cut(s) 565, 2263
BstXI CCANNNNNNTGG 1 cut(s) 2175
BstYI RGATCY 2 cut(s) 565, 2263
BstZI CGGCCG 1 cut(s) 111
Bsu15I ATCGAT 1 cut(s) 2420
BsuRI GGCC 5 cut(s) 113, 439, 1391, 1624, 2978
BsuTUI ATCGAT 1 cut(s) 2420
BtgI CCRYGG 1 cut(s) 108
BtsI GCAGTG 1 cut(s) 3003
BtsIMutI CAGTG 7 cut(s) 868, 1326, 1575, 2724, 2759, 3003, 3239
BveI ACCTGC 1 cut(s) 1919
Cac8I GCNNGC 5 cut(s) 344, 597, 2994, 3053, 3202
CaiI CAGNNNCTG 2 cut(s) 65, 3227
CciI TCATGA 1 cut(s) 3112
CfoI GCGC 4 cut(s) 107, 2409, 2736, 3206
Cfr13I GGNCC 5 cut(s) 703, 1390, 1582, 2301, 2977
Cfr42I CCGCGG 1 cut(s) 111
ClaI ATCGAT 1 cut(s) 2420
CseI GACGC 1 cut(s) 277
Csp6I GTAC 8 cut(s) 47, 211, 784, 2129, 2451, 3085, 3095, 3273
CviQI GTAC 8 cut(s) 47, 211, 784, 2129, 2451, 3085, 3095, 3273
DinI GGCGCC 1 cut(s) 106
DpnI GATC 9 cut(s) 567, 698, 1107, 1821, 2265, 2419, 2423, 2826, 2899
DpnII GATC 9 cut(s) 565, 696, 1105, 1819, 2263, 2417, 2421, 2824, 2897
DraI TTTAAA 3 cut(s) 615, 774, 1755
DraIII CACNNNGTG 1 cut(s) 863
EaeI YGGCCR 1 cut(s) 111
EagI CGGCCG 1 cut(s) 111
Eam1104I CTCTTC 1 cut(s) 2013
EarI CTCTTC 1 cut(s) 2013
Ecl136II GAGCTC 1 cut(s) 1061
EclXI CGGCCG 1 cut(s) 111
Eco130I CCWWGG 1 cut(s) 131
Eco147I AGGCCT 1 cut(s) 439
Eco24I GRGCYC 1 cut(s) 1063
Eco31I GGTCTC 1 cut(s) 741
Eco32I GATATC 4 cut(s) 627, 1978, 2577, 3034
Eco47I GGWCC 3 cut(s) 703, 1582, 2301
Eco52I CGGCCG 1 cut(s) 111
Eco53kI GAGCTC 1 cut(s) 1061
Eco57I CTGAAG 7 cut(s) 293, 294, 749, 764, 899, 1209, 1419
Eco72I CACGTG 1 cut(s) 1673
Eco88I CYCGRG 1 cut(s) 2124
EcoICRI GAGCTC 1 cut(s) 1061
EcoRI GAATTC 1 cut(s) 181
EcoRII CCWGG 2 cut(s) 1150, 2596
EcoRV GATATC 4 cut(s) 627, 1978, 2577, 3034
EcoT14I CCWWGG 1 cut(s) 131
EcoT22I ATGCAT 2 cut(s) 1384, 2233
EcoT38I GRGCYC 1 cut(s) 1063
EgeI GGCGCC 1 cut(s) 106
EheI GGCGCC 1 cut(s) 106
ErhI CCWWGG 1 cut(s) 131
Esp3I CGTCTC 1 cut(s) 2252
FalI AAGNNNNNCTT 2 cut(s) 2298, 2330
FaqI GGGAC 1 cut(s) 2810
FauI CCCGC 1 cut(s) 3193
FblI GTMKAC 1 cut(s) 141
FriOI GRGCYC 1 cut(s) 1063
FspBI CTAG 7 cut(s) 132, 563, 731, 1245, 1335, 1515, 1637
FspI TGCGCA 1 cut(s) 2735
GlaI GCGC 4 cut(s) 106, 2408, 2735, 3205
GsuI CTGGAG 3 cut(s) 513, 2619, 3350
HaeII RGCGCY 1 cut(s) 108
HaeIII GGCC 5 cut(s) 113, 439, 1391, 1624, 2978
HapII CCGG 5 cut(s) 150, 365, 2404, 2794, 3287
HgaI GACGC 1 cut(s) 277
HhaI GCGC 4 cut(s) 107, 2409, 2736, 3206
Hin1I GRCGYC 1 cut(s) 105
Hin6I GCGC 4 cut(s) 105, 2407, 2734, 3204
HinP1I GCGC 4 cut(s) 105, 2407, 2734, 3204
HincII GTYRAC 5 cut(s) 142, 723, 1460, 3069, 3256
HindII GTYRAC 5 cut(s) 142, 723, 1460, 3069, 3256
HindIII AAGCTT 2 cut(s) 157, 791
HpaII CCGG 5 cut(s) 150, 365, 2404, 2794, 3287
HphI GGTGA 6 cut(s) 131, 478, 1424, 1756, 2714, 2783
Hpy166II GTNNAC 8 cut(s) 142, 723, 1460, 1947, 2240, 2759, 3069, 3256
Hpy8I GTNNAC 8 cut(s) 142, 723, 1460, 1947, 2240, 2759, 3069, 3256
Hpy99I CGWCG 6 cut(s) 119, 128, 364, 436, 1390, 2031
HpyCH4III ACNGT 4 cut(s) 85, 119, 863, 1523
HpyCH4IV ACGT 4 cut(s) 219, 1672, 2912, 2928
HpyF10VI GCNNNNNNNGC 8 cut(s) 339, 436, 1152, 1388, 1621, 1630, 2779, 2998
HpySE526I ACGT 4 cut(s) 219, 1672, 2912, 2928
Hsp92I GRCGYC 1 cut(s) 105
HspAI GCGC 4 cut(s) 105, 2407, 2734, 3204
KasI GGCGCC 1 cut(s) 104
Kpn2I TCCGGA 1 cut(s) 3286
KpnI GGTACC 1 cut(s) 50
KspI CCGCGG 1 cut(s) 111
Kzo9I GATC 9 cut(s) 565, 696, 1105, 1819, 2263, 2417, 2421, 2824, 2897
LmnI GCTCC 6 cut(s) 409, 1294, 2119, 2341, 2767, 3331
Lsp1109I GCAGC 8 cut(s) 13, 527, 1063, 1584, 1770, 2447, 2757, 2903
MaeI CTAG 7 cut(s) 132, 563, 731, 1245, 1335, 1515, 1637
MaeII ACGT 4 cut(s) 219, 1672, 2912, 2928
MaeIII GTNAC 6 cut(s) 119, 503, 1119, 1616, 1860, 2389
MalI GATC 9 cut(s) 567, 698, 1107, 1821, 2265, 2419, 2423, 2826, 2899
MbiI CCGCTC 1 cut(s) 2255
MboI GATC 9 cut(s) 565, 696, 1105, 1819, 2263, 2417, 2421, 2824, 2897
MflI RGATCY 2 cut(s) 565, 2263
MhlI GDGCHC 4 cut(s) 1063, 1849, 2761, 3336
Mly113I GGCGCC 1 cut(s) 105
MlyI GAGTC 4 cut(s) 1325, 1333, 1661, 3187
MmeI TCCRAC 7 cut(s) 104, 366, 672, 1692, 2279, 2911, 3039
Mph1103I ATGCAT 2 cut(s) 1384, 2233
MroI TCCGGA 1 cut(s) 3286
MroXI GAANNNNTTC 2 cut(s) 1680, 2938
MslI CAYNNNNRTG 5 cut(s) 823, 864, 1476, 2173, 3046
MspA1I CMGCKG 2 cut(s) 110, 314
MspI CCGG 5 cut(s) 150, 365, 2404, 2794, 3287
MspR9I CCNGG 3 cut(s) 1152, 2598, 2795
Mva1269I GAATGC 5 cut(s) 344, 982, 1148, 1297, 2773
MvaI CCWGG 2 cut(s) 1152, 2598
MvnI CGCG 1 cut(s) 110
MwoI GCNNNNNNNGC 8 cut(s) 339, 436, 1152, 1388, 1621, 1630, 2779, 2998
NarI GGCGCC 1 cut(s) 105
NciI CCSGG 1 cut(s) 2795
NdeII GATC 9 cut(s) 565, 696, 1105, 1819, 2263, 2417, 2421, 2824, 2897
NmeAIII GCCGAG 1 cut(s) 451
NmuCI GTSAC 4 cut(s) 119, 503, 1616, 1860
NsbI TGCGCA 1 cut(s) 2735
NsiI ATGCAT 2 cut(s) 1384, 2233
NspI RCATGY 4 cut(s) 1127, 2174, 2235, 2757
NspV TTCGAA 2 cut(s) 2215, 2323
OliI CACNNNNGTG 1 cut(s) 2173
PaeR7I CTCGAG 1 cut(s) 2124
PagI TCATGA 1 cut(s) 3112
PaqCI CACCTGC 1 cut(s) 1919
PceI AGGCCT 1 cut(s) 439
PciI ACATGT 1 cut(s) 1123
PcsI WCGNNNNNNNCGW 1 cut(s) 123
PctI GAATGC 5 cut(s) 344, 982, 1148, 1297, 2773
PdmI GAANNNNTTC 2 cut(s) 1680, 2938
PfeI GAWTC 6 cut(s) 1178, 1747, 2212, 3109, 3283, 3292
PflFI GACNNNGTC 1 cut(s) 673
PflMI CCANNNNNTGG 2 cut(s) 536, 1741
PfoI TCCNGGA 1 cut(s) 2596
PleI GAGTC 4 cut(s) 1325, 1332, 1661, 3187
PluTI GGCGCC 1 cut(s) 108
PmaCI CACGTG 1 cut(s) 1673
PmlI CACGTG 1 cut(s) 1673
PpsI GAGTC 4 cut(s) 1325, 1332, 1661, 3187
Ppu21I YACGTR 2 cut(s) 1673, 2913
PscI ACATGT 1 cut(s) 1123
PshBI ATTAAT 2 cut(s) 294, 2189
Psp124BI GAGCTC 1 cut(s) 1063
Psp6I CCWGG 2 cut(s) 1150, 2596
PspCI CACGTG 1 cut(s) 1673
PspGI CCWGG 2 cut(s) 1150, 2596
PspPI GGNCC 5 cut(s) 703, 1390, 1582, 2301, 2977
PspXI VCTCGAGB 1 cut(s) 2124
PsrI GAACNNNNNNTAC 2 cut(s) 2483, 2515
PstI CTGCAG 1 cut(s) 1788
PstNI CAGNNNCTG 2 cut(s) 65, 3227
PsuI RGATCY 2 cut(s) 565, 2263
PsyI GACNNNGTC 1 cut(s) 673
RsaI GTAC 8 cut(s) 48, 212, 785, 2130, 2452, 3086, 3096, 3274
RsaNI GTAC 8 cut(s) 47, 211, 784, 2129, 2451, 3085, 3095, 3273
RseI CAYNNNNRTG 5 cut(s) 823, 864, 1476, 2173, 3046
SacI GAGCTC 1 cut(s) 1063
SacII CCGCGG 1 cut(s) 111
SalI GTCGAC 1 cut(s) 140
Sau3AI GATC 9 cut(s) 565, 696, 1105, 1819, 2263, 2417, 2421, 2824, 2897
Sau96I GGNCC 5 cut(s) 703, 1390, 1582, 2301, 2977
SchI GAGTC 4 cut(s) 1325, 1333, 1661, 3187
ScrFI CCNGG 3 cut(s) 1152, 2598, 2795
SduI GDGCHC 4 cut(s) 1063, 1849, 2761, 3336
SfcI CTRYAG 3 cut(s) 1302, 1784, 3300
SfoI GGCGCC 1 cut(s) 106
Sfr274I CTCGAG 1 cut(s) 2124
Sfr303I CCGCGG 1 cut(s) 111
SfuI TTCGAA 2 cut(s) 2215, 2323
SgrBI CCGCGG 1 cut(s) 111
SinI GGWCC 3 cut(s) 703, 1582, 2301
SlaI CTCGAG 1 cut(s) 2124
SmiI ATTTAAAT 1 cut(s) 615
SmiMI CAYNNNNRTG 5 cut(s) 823, 864, 1476, 2173, 3046
SmlI CTYRAG 4 cut(s) 413, 476, 2124, 3195
SmoI CTYRAG 4 cut(s) 413, 476, 2124, 3195
SseBI AGGCCT 1 cut(s) 439
SsiI CCGC 7 cut(s) 102, 108, 110, 259, 314, 2255, 3200
SspDI GGCGCC 1 cut(s) 104
SspI AATATT 3 cut(s) 2182, 2273, 3013
SspMI CTAG 7 cut(s) 132, 563, 731, 1245, 1335, 1515, 1637
SstI GAGCTC 1 cut(s) 1063
StuI AGGCCT 1 cut(s) 439
StyD4I CCNGG 3 cut(s) 1150, 2596, 2793
StyI CCWWGG 1 cut(s) 131
SwaI ATTTAAAT 1 cut(s) 615
TaaI ACNGT 4 cut(s) 85, 119, 863, 1523
TaiI ACGT 4 cut(s) 222, 1675, 2915, 2931
TaqII GACCGA 1 cut(s) 3159
TatI WGTACW 2 cut(s) 210, 783
TauI GCSGC 4 cut(s) 105, 110, 113, 317
TfiI GAWTC 6 cut(s) 1178, 1747, 2212, 3109, 3283, 3292
TscAI CASTG 7 cut(s) 868, 1326, 1582, 2724, 2766, 3003, 3246
TseFI GTSAC 4 cut(s) 119, 503, 1616, 1860
TseI GCWGC 8 cut(s) 26, 540, 1051, 1572, 1783, 2435, 2770, 2916
Tsp45I GTSAC 4 cut(s) 119, 503, 1616, 1860
TspGWI ACGGA 6 cut(s) 1775, 2276, 2492, 3072, 3164, 3290
TspRI CASTG 7 cut(s) 868, 1326, 1582, 2724, 2766, 3003, 3246
Tth111I GACNNNGTC 1 cut(s) 673
Van91I CCANNNNNTGG 2 cut(s) 536, 1741
VneI GTGCAC 1 cut(s) 2757
VpaK11BI GGWCC 3 cut(s) 703, 1582, 2301
VspI ATTAAT 2 cut(s) 294, 2189
XapI RAATTY 6 cut(s) 181, 589, 1309, 2032, 3078, 3142
XbaI TCTAGA 1 cut(s) 562
XceI RCATGY 4 cut(s) 1127, 2174, 2235, 2757
XcmI CCANNNNNNNNNTGG 1 cut(s) 1592
XhoI CTCGAG 1 cut(s) 2124
XmaJI CCTAGG 1 cut(s) 131
XmiI GTMKAC 1 cut(s) 141
XmnI GAANNNNTTC 2 cut(s) 1680, 2938
XspI CTAG 7 cut(s) 132, 563, 731, 1245, 1335, 1515, 1637
Zsp2I ATGCAT 2 cut(s) 1384, 2233
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.