pycom16g17650

Targeting protein for Xklp2 (TPX2)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0008351
Physical Location & Seq
Reverse (-)
13905852 .. 13907821
1970 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1086 bp
ATGGACAAGGAATCAGAATGTGTCACAAATTACTCAGAAGGTCTTTCTCATGATTCAAATCATGAGAGAATCCCCAGTGATCACGTGATTTCAGAGTCATATGAGCATATTAATGGGGATCGTGAACAACAAAATTTGGAAGAAAGTCTTGAAGTAAAGGAGTATGAAGTGAAAGAATGTACTACAGAAAACTCTGTTGATGTTTCTCAACTGAGTCTTATTGAAAAATTTAGTGAGGATCAGAATGTAGTAAGGTCGAACTCTGATAATGACCAGCCTTTGGATAAGGTTCAACTGGATTCTGGGAAAACAAAGGTTAGTAGTAAGTCACTAGCATCTGCAAAACGTGCATCAAAACCTACTGCGGGAAATGCTAGAACAAAACACACTGTTCCACAGCCATTTACCTTAGCGACTGAAAAGCGTGCTTCATGTGGAACTCGTCCAGTCAGTGCTGTACTTGATTGCGGTAATGGTGCCAATAAATTACCCAACACAAGTGATCTGCGTCATCTTAATACCACAAAGCATAACCAGCCAATATCACCTTTGGTACCAAGGATGCCAATGCAACCAGATAAGAAGAAGCATTCTGATGAGGAAGATTCCTGTTCTGTTGCTTCCACGTTAGAGCAGAAGCACCAAGCATTGGAGGTGCAAAAAACTGAAAGCGATGCAAGGACCAAGGAAGAGAAGGAGGCGGCTATCAAGCAACTGAGAAAGAGTTTAACATTTAAGGCAAACCCGATGCCAAGCTTCTATCACGAGGGACCTCCACCTAAAACTGAACTTAAAAAGCTGCCACCAACTCGTGCAAAGTCACCAAAGCTTGGCCGGAGGATGAGCTGCAGTGAAACAGTCAAAGTATCTCGAGGAGACAGAATGAAAGGAAATCGTCACAGTATGGGTAGTTACAAAGAAGACACCACTTATGCTGGTTCCCTGAAAAGTGAGGGTCAGATCAACATCCAAATTGGTAATGCCGTTTGCGAGTTCATTGATGAGACTAAGAAAGTGGACGAGATAAACAAGTTTGTTCCGGCAAACCTAAATGGACAGCACATGGACATGTATGTTCAATCTTGA

Protein Analysis

362

Amino Acids

40.25

Weight (kDa)

6.87

Isoelectric Point (pI)

53.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPX2 PF06886 209 - 262 2.9e-16 Targeting protein for Xklp2 (TPX2) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 553
AccB1I GGYRCC 2 cut(s) 476, 553
AccB7I CCANNNNNTGG 3 cut(s) 280, 649, 830
AciI CCGC 3 cut(s) 365, 468, 701
AclWI GGATC 2 cut(s) 126, 246
AcoI YGGCCR 1 cut(s) 832
AcsI RAATTY 2 cut(s) 133, 227
AcvI CACGTG 1 cut(s) 85
AfaI GTAC 3 cut(s) 181, 459, 555
AfiI CCNNNNNNNGG 4 cut(s) 280, 365, 649, 830
AflIII ACRYGT 1 cut(s) 1068
AgsI TTSAA 5 cut(s) 57, 152, 224, 293, 1079
AluBI AGCT 4 cut(s) 756, 799, 829, 846
AluI AGCT 4 cut(s) 756, 799, 829, 846
Alw26I GTCTC 2 cut(s) 870, 998
AlwI GGATC 2 cut(s) 126, 246
Ama87I CYCGRG 1 cut(s) 870
AoxI GGCC 1 cut(s) 832
ApeKI GCWGC 2 cut(s) 799, 846
ApoI RAATTY 2 cut(s) 133, 227
AseI ATTAAT 1 cut(s) 111
Asp718I GGTACC 1 cut(s) 553
AspS9I GGNCC 2 cut(s) 681, 770
AsuHPI GGTGA 2 cut(s) 537, 813
AvaI CYCGRG 1 cut(s) 870
AvaII GGWCC 2 cut(s) 681, 770
BanI GGYRCC 2 cut(s) 476, 553
BauI CACGAG 2 cut(s) 764, 810
BbrPI CACGTG 1 cut(s) 85
BbsI GAAGAC 1 cut(s) 927
BbvI GCAGC 2 cut(s) 786, 833
BceAI ACGGC 1 cut(s) 968
BclI TGATCA 1 cut(s) 79
BcoDI GTCTC 2 cut(s) 870, 998
BfaI CTAG 2 cut(s) 332, 375
BfmI CTRYAG 2 cut(s) 183, 847
BisI GCNGC 3 cut(s) 702, 800, 847
BlsI GCNGC 3 cut(s) 703, 801, 848
Bme18I GGWCC 2 cut(s) 681, 770
BmeT110I CYCGRG 1 cut(s) 870
BmgT120I GGNCC 2 cut(s) 681, 770
BmiI GGNNCC 4 cut(s) 478, 555, 771, 940
BmrI ACTGGG 1 cut(s) 69
BmsI GCATC 5 cut(s) 344, 359, 552, 664, 738
BmuI ACTGGG 1 cut(s) 69
BpiI GAAGAC 1 cut(s) 927
Bpu10I CCTNAGC 1 cut(s) 409
BsaAI YACGTR 1 cut(s) 85
BsaBI GATNNNNATC 2 cut(s) 57, 965
BsaJI CCNNGG 2 cut(s) 557, 684
Bsc4I CCNNNNNNNGG 4 cut(s) 280, 365, 649, 830
Bse1I ACTGG 3 cut(s) 75, 300, 446
Bse8I GATNNNNATC 2 cut(s) 57, 965
BseDI CCNNGG 2 cut(s) 557, 684
BseGI GGATG 3 cut(s) 567, 846, 966
BseJI GATNNNNATC 2 cut(s) 57, 965
BseLI CCNNNNNNNGG 4 cut(s) 280, 365, 649, 830
BseMII CTCAG 3 cut(s) 48, 203, 707
BseNI ACTGG 3 cut(s) 75, 300, 446
BseRI GAGGAG 1 cut(s) 888
BseXI GCAGC 2 cut(s) 786, 833
BshFI GGCC 1 cut(s) 834
BshNI GGYRCC 2 cut(s) 476, 553
BsiHKCI CYCGRG 1 cut(s) 870
BsiSI CCGG 2 cut(s) 835, 1040
BslFI GGGAC 1 cut(s) 783
BslI CCNNNNNNNGG 4 cut(s) 280, 365, 649, 830
BsmAI GTCTC 2 cut(s) 870, 998
BsmFI GGGAC 1 cut(s) 783
BsmI GAATGC 1 cut(s) 589
BsnI GGCC 1 cut(s) 834
BsoBI CYCGRG 1 cut(s) 870
Bsp143I GATC 5 cut(s) 79, 118, 238, 502, 960
BspACI CCGC 3 cut(s) 365, 468, 701
BspANI GGCC 1 cut(s) 834
BspCNI CTCAG 3 cut(s) 47, 204, 708
BspHI TCATGA 2 cut(s) 49, 61
BspLI GGNNCC 4 cut(s) 478, 555, 771, 940
BspMAI CTGCAG 1 cut(s) 851
BspPI GGATC 2 cut(s) 126, 246
BspT107I GGYRCC 2 cut(s) 476, 553
BsrI ACTGG 3 cut(s) 75, 300, 446
BssECI CCNNGG 2 cut(s) 557, 684
BssMI GATC 5 cut(s) 79, 118, 238, 502, 960
BssSI CACGAG 2 cut(s) 764, 810
BssT1I CCWWGG 2 cut(s) 557, 684
Bst2BI CACGAG 2 cut(s) 764, 810
Bst4CI ACNGT 3 cut(s) 391, 859, 902
Bst6I CTCTTC 1 cut(s) 684
BstAPI GCANNNNNTGC 1 cut(s) 347
BstBAI YACGTR 1 cut(s) 85
BstC8I GCNNGC 1 cut(s) 426
BstDEI CTNAG 5 cut(s) 34, 212, 409, 716, 1008
BstF5I GGATG 3 cut(s) 567, 846, 966
BstKTI GATC 5 cut(s) 82, 121, 241, 505, 963
BstMAI GTCTC 2 cut(s) 870, 998
BstMBI GATC 5 cut(s) 79, 118, 238, 502, 960
BstMWI GCNNNNNNNGC 3 cut(s) 347, 371, 535
BstNSI RCATGY 1 cut(s) 1072
BstSFI CTRYAG 2 cut(s) 183, 847
BstV1I GCAGC 2 cut(s) 786, 833
BstV2I GAAGAC 1 cut(s) 927
BsuRI GGCC 1 cut(s) 834
BtgZI GCGATG 1 cut(s) 687
BtsCI GGATG 3 cut(s) 567, 846, 966
BtsI GCAGTG 1 cut(s) 856
BtsIMutI CAGTG 4 cut(s) 82, 387, 457, 856
Cac8I GCNNGC 1 cut(s) 426
CciI TCATGA 2 cut(s) 49, 61
Cfr13I GGNCC 2 cut(s) 681, 770
CseI GACGC 1 cut(s) 497
Csp6I GTAC 3 cut(s) 180, 458, 554
CviAII CATG 5 cut(s) 50, 62, 432, 1063, 1069
CviJI RGCY 9 cut(s) 277, 400, 538, 704, 756, 799, 829, 834, 846
CviKI_1 RGCY 9 cut(s) 277, 400, 538, 704, 756, 799, 829, 834, 846
CviQI GTAC 3 cut(s) 180, 458, 554
DdeI CTNAG 5 cut(s) 34, 212, 409, 716, 1008
DpnI GATC 5 cut(s) 81, 120, 240, 504, 962
DpnII GATC 5 cut(s) 79, 118, 238, 502, 960
EaeI YGGCCR 1 cut(s) 832
Eam1104I CTCTTC 1 cut(s) 684
EarI CTCTTC 1 cut(s) 684
Eco130I CCWWGG 2 cut(s) 557, 684
Eco47I GGWCC 2 cut(s) 681, 770
Eco72I CACGTG 1 cut(s) 85
Eco88I CYCGRG 1 cut(s) 870
EcoO109I RGGNCCY 1 cut(s) 770
EcoT14I CCWWGG 2 cut(s) 557, 684
ErhI CCWWGG 2 cut(s) 557, 684
FaeI CATG 5 cut(s) 53, 65, 435, 1066, 1072
FalI AAGNNNNNCTT 2 cut(s) 132, 164
FaqI GGGAC 1 cut(s) 783
FatI CATG 5 cut(s) 49, 61, 431, 1062, 1068
FauI CCCGC 1 cut(s) 358
FauNDI CATATG 1 cut(s) 100
FbaI TGATCA 1 cut(s) 79
Fnu4HI GCNGC 3 cut(s) 702, 800, 847
FokI GGATG 3 cut(s) 574, 853, 953
Fsp4HI GCNGC 3 cut(s) 702, 800, 847
FspBI CTAG 2 cut(s) 332, 375
GluI GCNGC 3 cut(s) 702, 800, 847
HaeIII GGCC 1 cut(s) 834
HapII CCGG 2 cut(s) 835, 1040
HgaI GACGC 1 cut(s) 497
Hin1II CATG 5 cut(s) 53, 65, 435, 1066, 1072
HindIII AAGCTT 2 cut(s) 754, 827
HinfI GANTC 7 cut(s) 11, 53, 69, 95, 214, 299, 605
HpaII CCGG 2 cut(s) 835, 1040
HphI GGTGA 2 cut(s) 537, 813
Hpy166II GTNNAC 2 cut(s) 125, 1018
Hpy188I TCNGA 7 cut(s) 16, 37, 94, 243, 265, 595, 960
Hpy188III TCNNGA 7 cut(s) 50, 62, 122, 149, 764, 870, 1083
Hpy8I GTNNAC 2 cut(s) 125, 1018
HpyAV CCTTC 2 cut(s) 32, 688
HpyCH4III ACNGT 3 cut(s) 391, 859, 902
HpyCH4IV ACGT 3 cut(s) 84, 346, 626
HpyCH4V TGCA 7 cut(s) 341, 350, 571, 658, 677, 815, 849
HpyF10VI GCNNNNNNNGC 3 cut(s) 347, 371, 535
HpyF3I CTNAG 5 cut(s) 34, 212, 409, 716, 1008
HpySE526I ACGT 3 cut(s) 84, 346, 626
Hsp92II CATG 5 cut(s) 53, 65, 435, 1066, 1072
KpnI GGTACC 1 cut(s) 557
Ksp22I TGATCA 1 cut(s) 79
Kzo9I GATC 5 cut(s) 79, 118, 238, 502, 960
Lsp1109I GCAGC 2 cut(s) 786, 833
LweI GCATC 5 cut(s) 344, 359, 552, 664, 738
MaeI CTAG 2 cut(s) 332, 375
MaeII ACGT 3 cut(s) 84, 346, 626
MaeIII GTNAC 5 cut(s) 22, 327, 819, 896, 911
MalI GATC 5 cut(s) 81, 120, 240, 504, 962
MboI GATC 5 cut(s) 79, 118, 238, 502, 960
MboII GAAGA 5 cut(s) 152, 595, 614, 701, 932
MluCI AATT 5 cut(s) 28, 133, 227, 485, 972
MlyI GAGTC 2 cut(s) 104, 223
MnlI CCTC 9 cut(s) 229, 592, 646, 691, 760, 783, 831, 866, 946
MseI TTAA 5 cut(s) 111, 516, 728, 735, 792
MslI CAYNNNNRTG 3 cut(s) 111, 594, 1067
MspI CCGG 2 cut(s) 835, 1040
Mva1269I GAATGC 1 cut(s) 589
MwoI GCNNNNNNNGC 3 cut(s) 347, 371, 535
NdeI CATATG 1 cut(s) 100
NdeII GATC 5 cut(s) 79, 118, 238, 502, 960
NlaIII CATG 5 cut(s) 53, 65, 435, 1066, 1072
NlaIV GGNNCC 4 cut(s) 478, 555, 771, 940
NmuCI GTSAC 4 cut(s) 22, 327, 819, 896
NspI RCATGY 1 cut(s) 1072
PaeR7I CTCGAG 1 cut(s) 870
PagI TCATGA 2 cut(s) 49, 61
PciI ACATGT 1 cut(s) 1068
PctI GAATGC 1 cut(s) 589
PfeI GAWTC 5 cut(s) 11, 53, 69, 299, 605
PflMI CCANNNNNTGG 3 cut(s) 280, 649, 830
PkrI GCNGC 3 cut(s) 703, 801, 848
PleI GAGTC 2 cut(s) 103, 222
PmaCI CACGTG 1 cut(s) 85
PmlI CACGTG 1 cut(s) 85
PpsI GAGTC 2 cut(s) 103, 222
Ppu21I YACGTR 1 cut(s) 85
PpuMI RGGWCCY 1 cut(s) 770
PscI ACATGT 1 cut(s) 1068
PshBI ATTAAT 1 cut(s) 111
Psp5II RGGWCCY 1 cut(s) 770
PspCI CACGTG 1 cut(s) 85
PspN4I GGNNCC 4 cut(s) 478, 555, 771, 940
PspPI GGNCC 2 cut(s) 681, 770
PspPPI RGGWCCY 1 cut(s) 770
PstI CTGCAG 1 cut(s) 851
RsaI GTAC 3 cut(s) 181, 459, 555
RsaNI GTAC 3 cut(s) 180, 458, 554
RseI CAYNNNNRTG 3 cut(s) 111, 594, 1067
SaqAI TTAA 5 cut(s) 111, 516, 728, 735, 792
SatI GCNGC 3 cut(s) 702, 800, 847
Sau3AI GATC 5 cut(s) 79, 118, 238, 502, 960
Sau96I GGNCC 2 cut(s) 681, 770
SchI GAGTC 2 cut(s) 104, 223
SfaNI GCATC 5 cut(s) 344, 359, 552, 664, 738
SfcI CTRYAG 2 cut(s) 183, 847
Sfr274I CTCGAG 1 cut(s) 870
SinI GGWCC 2 cut(s) 681, 770
SlaI CTCGAG 1 cut(s) 870
SmiMI CAYNNNNRTG 3 cut(s) 111, 594, 1067
SmlI CTYRAG 1 cut(s) 870
SmoI CTYRAG 1 cut(s) 870
Sse9I AATT 5 cut(s) 28, 133, 227, 485, 972
SsiI CCGC 3 cut(s) 365, 468, 701
SspMI CTAG 2 cut(s) 332, 375
StyI CCWWGG 2 cut(s) 557, 684
TaaI ACNGT 3 cut(s) 391, 859, 902
TaiI ACGT 3 cut(s) 87, 349, 629
TaqI TCGA 2 cut(s) 257, 871
TasI AATT 5 cut(s) 28, 133, 227, 485, 972
TatI WGTACW 2 cut(s) 179, 457
TauI GCSGC 1 cut(s) 704
TfiI GAWTC 5 cut(s) 11, 53, 69, 299, 605
Tru1I TTAA 5 cut(s) 111, 516, 728, 735, 792
Tru9I TTAA 5 cut(s) 111, 516, 728, 735, 792
TscAI CASTG 4 cut(s) 82, 394, 457, 856
TseFI GTSAC 4 cut(s) 22, 327, 819, 896
TseI GCWGC 2 cut(s) 799, 846
Tsp45I GTSAC 4 cut(s) 22, 327, 819, 896
TspDTI ATGAA 4 cut(s) 180, 420, 899, 985
TspRI CASTG 4 cut(s) 82, 394, 457, 856
Van91I CCANNNNNTGG 3 cut(s) 280, 649, 830
VpaK11BI GGWCC 2 cut(s) 681, 770
VspI ATTAAT 1 cut(s) 111
XapI RAATTY 2 cut(s) 133, 227
XceI RCATGY 1 cut(s) 1072
XhoI CTCGAG 1 cut(s) 870
XspI CTAG 2 cut(s) 332, 375
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.