pycom16g20290

Endonuclease I

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0003467
Physical Location & Seq
Reverse (-)
18029095 .. 18030777
1683 bp
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UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 690 bp
ATGCAGCATCCCGTTGTGGTGGCCACCAAATTCCACTGCACAAAATCTGTCGTCGACAGCACCGCCGTCCGTAAGCAGAGCGCCACCTTCTCCCGCAGCCGCGACGAGCACTCCAAAACCTCATTCCAATTAGCTTGCAACTATCGCAACCCAGATAATAATTTGGTCTCTCCAACCACTTCCCCCATTTCTACAAGATGGAATTACAGAAAATGCATCAGTGTTTCTTGGAGATGGGTTTCTCAGATCATCTCTTTTTATTTGTTGTTTCTCAATGTTGTTGCTGATGCTGCGGCATTTGATAATCCATCTCCTTCTTTTATGTATGGTTGTGAAGATGTTAGCAATTATTATGACAGTGTGGAGAATTTAGAGGGCAAATCGCTGAAGAAAAAATTGAATTCCATTATTTCCAGGCACCAATCTTTATCCTACAGAGAGGTGTGGAATGCTCTCAAGATTCTTGATGCTTCGGATGTTGATAACCCAGAAGCTTCGTTGGGAATAGTTGAAGTTTACTCCTTGAGGGTTGTACCCAAGTCTTTAGCTGGGAAACCCGAAGGATGGAATAGGGAGCATCTATGGCCTCGTTCTTTTGGGTTAAAAGATGGTGCGTCTTTGACAGATATACACAACATTCGCCCAGCAGATGTGAATGGTAAGATGGGCCACTGCCTGTATGTATTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Endonuclease_1

Protein Analysis

230

Amino Acids

25.86

Weight (kDa)

8.92

Isoelectric Point (pI)

49.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Endonuclease_1 PF04231 126 - 227 2.1e-09 Endonuclease I
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 417
AccI GTMKAC 1 cut(s) 54
AccII CGCG 1 cut(s) 102
AciI CCGC 4 cut(s) 63, 94, 100, 293
AcoI YGGCCR 1 cut(s) 21
AcsI RAATTY 3 cut(s) 29, 367, 400
AcuI CTGAAG 1 cut(s) 407
AfaI GTAC 1 cut(s) 534
AfiI CCNNNNNNNGG 1 cut(s) 564
AgsI TTSAA 2 cut(s) 400, 512
AjnI CCWGG 1 cut(s) 413
AjuI GAANNNNNNNTTGG 2 cut(s) 107, 139
AluBI AGCT 3 cut(s) 134, 494, 548
AluI AGCT 3 cut(s) 134, 494, 548
Alw21I GWGCWC 1 cut(s) 111
Alw26I GTCTC 1 cut(s) 172
AoxI GGCC 3 cut(s) 21, 584, 667
ApeKI GCWGC 3 cut(s) 4, 96, 290
ApoI RAATTY 3 cut(s) 29, 367, 400
AspLEI GCGC 1 cut(s) 83
AspS9I GGNCC 1 cut(s) 667
BalI TGGCCA 1 cut(s) 23
BanI GGYRCC 1 cut(s) 417
Bbv12I GWGCWC 1 cut(s) 111
BbvI GCAGC 3 cut(s) 16, 108, 277
BccI CCATC 6 cut(s) 192, 228, 316, 558, 602, 658
BceAI ACGGC 1 cut(s) 50
BciT130I CCWGG 1 cut(s) 415
BcoDI GTCTC 1 cut(s) 172
BfmI CTRYAG 1 cut(s) 433
BfoI RGCGCY 1 cut(s) 84
BisI GCNGC 5 cut(s) 5, 97, 100, 291, 294
BlsI GCNGC 5 cut(s) 6, 98, 101, 292, 295
Bme1390I CCNGG 1 cut(s) 415
BmgT120I GGNCC 1 cut(s) 667
BmiI GGNNCC 1 cut(s) 419
BmrFI CCNGG 1 cut(s) 415
BmsI GCATC 5 cut(s) 16, 225, 277, 457, 586
BpuEI CTTGAG 2 cut(s) 440, 544
BsaI GGTCTC 1 cut(s) 172
BsaXI ACNNNNNCTCC 2 cut(s) 95, 125
Bsc4I CCNNNNNNNGG 1 cut(s) 564
BseBI CCWGG 1 cut(s) 415
BseGI GGATG 3 cut(s) 7, 481, 569
BseLI CCNNNNNNNGG 1 cut(s) 564
BseMII CTCAG 1 cut(s) 257
BseXI GCAGC 3 cut(s) 16, 108, 277
BseYI CCCAGC 2 cut(s) 548, 643
BsgI GTGCAG 1 cut(s) 22
Bsh1236I CGCG 1 cut(s) 102
BshFI GGCC 3 cut(s) 23, 586, 669
BshNI GGYRCC 1 cut(s) 417
BsiHKAI GWGCWC 1 cut(s) 111
BslI CCNNNNNNNGG 1 cut(s) 564
BsmAI GTCTC 1 cut(s) 172
BsmI GAATGC 1 cut(s) 454
BsnI GGCC 3 cut(s) 23, 586, 669
Bso31I GGTCTC 1 cut(s) 172
Bsp1286I GDGCHC 1 cut(s) 111
Bsp143I GATC 1 cut(s) 246
BspACI CCGC 4 cut(s) 63, 94, 100, 293
BspANI GGCC 3 cut(s) 23, 586, 669
BspCNI CTCAG 1 cut(s) 256
BspFNI CGCG 1 cut(s) 102
BspLI GGNNCC 1 cut(s) 419
BspT107I GGYRCC 1 cut(s) 417
BspTNI GGTCTC 1 cut(s) 172
BssMI GATC 1 cut(s) 246
Bst2UI CCWGG 1 cut(s) 415
Bst4CI ACNGT 1 cut(s) 359
BstC8I GCNNGC 1 cut(s) 136
BstDEI CTNAG 1 cut(s) 243
BstF5I GGATG 3 cut(s) 7, 481, 569
BstFNI CGCG 1 cut(s) 102
BstH2I RGCGCY 1 cut(s) 84
BstHHI GCGC 1 cut(s) 83
BstKTI GATC 1 cut(s) 249
BstMAI GTCTC 1 cut(s) 172
BstMBI GATC 1 cut(s) 246
BstMWI GCNNNNNNNGC 3 cut(s) 144, 290, 583
BstNI CCWGG 1 cut(s) 415
BstSCI CCNGG 1 cut(s) 413
BstSFI CTRYAG 1 cut(s) 433
BstUI CGCG 1 cut(s) 102
BstV1I GCAGC 3 cut(s) 16, 108, 277
BsuRI GGCC 3 cut(s) 23, 586, 669
BtsCI GGATG 3 cut(s) 7, 481, 569
BtsI GCAGTG 2 cut(s) 34, 670
BtsIMutI CAGTG 4 cut(s) 34, 226, 364, 670
Cac8I GCNNGC 1 cut(s) 136
CfoI GCGC 1 cut(s) 83
Cfr13I GGNCC 1 cut(s) 667
CseI GACGC 1 cut(s) 603
Csp6I GTAC 1 cut(s) 533
CviJI RGCY 7 cut(s) 23, 99, 134, 494, 548, 586, 669
CviKI_1 RGCY 7 cut(s) 23, 99, 134, 494, 548, 586, 669
CviQI GTAC 1 cut(s) 533
DdeI CTNAG 1 cut(s) 243
DpnI GATC 1 cut(s) 248
DpnII GATC 1 cut(s) 246
EaeI YGGCCR 1 cut(s) 21
Eco31I GGTCTC 1 cut(s) 172
Eco57I CTGAAG 1 cut(s) 407
EcoRI GAATTC 1 cut(s) 400
EcoRII CCWGG 1 cut(s) 413
EcoT22I ATGCAT 1 cut(s) 218
FaiI YATR 6 cut(s) 323, 327, 354, 583, 629, 681
FauI CCCGC 1 cut(s) 101
FblI GTMKAC 1 cut(s) 54
Fnu4HI GCNGC 5 cut(s) 5, 97, 100, 291, 294
FokI GGATG 2 cut(s) 488, 576
Fsp4HI GCNGC 5 cut(s) 5, 97, 100, 291, 294
GlaI GCGC 1 cut(s) 82
GluI GCNGC 5 cut(s) 5, 97, 100, 291, 294
GsaI CCCAGC 2 cut(s) 552, 647
HaeII RGCGCY 1 cut(s) 84
HaeIII GGCC 3 cut(s) 23, 586, 669
HgaI GACGC 1 cut(s) 603
HhaI GCGC 1 cut(s) 83
Hin6I GCGC 1 cut(s) 81
HinP1I GCGC 1 cut(s) 81
HincII GTYRAC 1 cut(s) 55
HindII GTYRAC 1 cut(s) 55
HindIII AAGCTT 1 cut(s) 492
HinfI GANTC 1 cut(s) 460
Hpy166II GTNNAC 2 cut(s) 55, 517
Hpy188I TCNGA 2 cut(s) 246, 475
Hpy188III TCNNGA 2 cut(s) 457, 464
Hpy8I GTNNAC 2 cut(s) 55, 517
Hpy99I CGWCG 2 cut(s) 56, 107
HpyAV CCTTC 3 cut(s) 97, 324, 554
HpyCH4III ACNGT 1 cut(s) 359
HpyCH4V TGCA 4 cut(s) 4, 39, 138, 216
HpyF10VI GCNNNNNNNGC 3 cut(s) 144, 290, 583
HpyF3I CTNAG 1 cut(s) 243
HspAI GCGC 1 cut(s) 81
Kzo9I GATC 1 cut(s) 246
LmnI GCTCC 1 cut(s) 574
LpnPI CCDG 6 cut(s) 165, 400, 427, 501, 534, 657
Lsp1109I GCAGC 3 cut(s) 16, 108, 277
LweI GCATC 5 cut(s) 16, 225, 277, 457, 586
MalI GATC 1 cut(s) 248
MboI GATC 1 cut(s) 246
MboII GAAGA 2 cut(s) 347, 400
MhlI GDGCHC 1 cut(s) 111
MlsI TGGCCA 1 cut(s) 23
MluCI AATT 8 cut(s) 29, 128, 160, 202, 346, 367, 395, 400
MluNI TGGCCA 1 cut(s) 23
MmeI TCCRAC 1 cut(s) 197
MnlI CCTC 5 cut(s) 130, 367, 433, 519, 597
Mox20I TGGCCA 1 cut(s) 23
Mph1103I ATGCAT 1 cut(s) 218
MscI TGGCCA 1 cut(s) 23
MseI TTAA 1 cut(s) 602
Msp20I TGGCCA 1 cut(s) 23
MspR9I CCNGG 1 cut(s) 415
Mva1269I GAATGC 1 cut(s) 454
MvaI CCWGG 1 cut(s) 415
MvnI CGCG 1 cut(s) 102
MwoI GCNNNNNNNGC 3 cut(s) 144, 290, 583
NdeII GATC 1 cut(s) 246
NlaIV GGNNCC 1 cut(s) 419
NsiI ATGCAT 1 cut(s) 218
PctI GAATGC 1 cut(s) 454
PfeI GAWTC 1 cut(s) 460
PkrI GCNGC 5 cut(s) 6, 98, 101, 292, 295
Psp6I CCWGG 1 cut(s) 413
PspFI CCCAGC 2 cut(s) 548, 643
PspGI CCWGG 1 cut(s) 413
PspN4I GGNNCC 1 cut(s) 419
PspPI GGNCC 1 cut(s) 667
RsaI GTAC 1 cut(s) 534
RsaNI GTAC 1 cut(s) 533
SalI GTCGAC 1 cut(s) 53
SaqAI TTAA 1 cut(s) 602
SatI GCNGC 5 cut(s) 5, 97, 100, 291, 294
Sau3AI GATC 1 cut(s) 246
Sau96I GGNCC 1 cut(s) 667
ScrFI CCNGG 1 cut(s) 415
SduI GDGCHC 1 cut(s) 111
SetI ASST 6 cut(s) 89, 122, 136, 444, 496, 550
SfaNI GCATC 5 cut(s) 16, 225, 277, 457, 586
SfcI CTRYAG 1 cut(s) 433
SmlI CTYRAG 2 cut(s) 455, 523
SmoI CTYRAG 2 cut(s) 455, 523
Sse9I AATT 8 cut(s) 29, 128, 160, 202, 346, 367, 395, 400
SsiI CCGC 4 cut(s) 63, 94, 100, 293
StyD4I CCNGG 1 cut(s) 413
TaaI ACNGT 1 cut(s) 359
TaqI TCGA 1 cut(s) 54
TasI AATT 8 cut(s) 29, 128, 160, 202, 346, 367, 395, 400
TauI GCSGC 2 cut(s) 102, 296
TfiI GAWTC 1 cut(s) 460
Tru1I TTAA 1 cut(s) 602
Tru9I TTAA 1 cut(s) 602
TscAI CASTG 4 cut(s) 41, 226, 364, 677
TseI GCWGC 3 cut(s) 4, 96, 290
TspGWI ACGGA 1 cut(s) 59
TspRI CASTG 4 cut(s) 41, 226, 364, 677
XapI RAATTY 3 cut(s) 29, 367, 400
XmiI GTMKAC 1 cut(s) 54
Zsp2I ATGCAT 1 cut(s) 218
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.