pycom16g20330

ABC transporter I family member

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0014429
Physical Location & Seq
Forward (+)
18086742 .. 18088982
2241 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom16g20330.1

Sequence Viewer

Length: 621 bp
ATGGGGGTCATTGGCCCCAGCGGCAGCGGCAAGTCCACGCTGCTTCGAGCTCTCAACCGCCTCTGGGAGCCGCCATCTGGGACTGTCTTCTTGGACGGTCGTGATATTCTGGACGTCGACGTTCTCAGCCTCCGCCGCAAGGTCGGCATGCTCTTCCAGCTTCCCGTTCTCTTTGAAGGCACAGTTGCAGACAATATACGTTATGGCCCTCAATTAAAGGGGAAGAAGCTGAGTGACGAGGACGTTCACAAGTTGCTGACACTTGTAGACCTTGATTCATCTTTTTCCACCAAGACTGGTTCTGAAATGTCTGTGGGTCAGTCCCAAAGAGTTGCACTTGCTAGGACCCTCGCCAATTCCCCTGAGGTTTTGCTGTTGGATGAGCCAACAAGTGCATTGGATCCAATATCAACAGAGCGCATAGAAGGTGCTTTGGAGAAGCTGAAGCAGAAACAGGGGATGACCATTGTGATGGTCTCTCACAGCATCAAACAAATCCAGAGGATTGCTGATATTGTTTGCCTACTTGTGGATGGGGAAATTGTTGAAGTTCTCAAACCAGACCAACTATCTCAAGCCCAGCATCCCATGGCACTACGCTTTCTTGAGCTCAGCTCTTAA

Protein Analysis

207

Amino Acids

22.51

Weight (kDa)

5.67

Isoelectric Point (pI)

37.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ABC_tran PF00005 2 - 131 1.2e-29 ABC transporter
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0014429)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G67940
fragaria_vesca FvH4_4g06671 FvH4_4g06671
malus_domestica MD13G1237100.v1.1 MD16G1242500.v1.1
prunus_persica Prupe.1G069500_v2.0.a1
pyrus_communis pycom16g20320 pycom16g20330
rosa_chinensis RchiOBHm_Chr4g0398581
rosa_laevigata RLG00000009342
rosa_roxburghii Rroxscaffold_5G00343550
rosa_rugosa Rorug04G0009500 Rorug04G0009600.1
rosa_samantha Rh4AG087500 Rh4BG084100 Rh4CG095700 Rh4DG079000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 117
AccI GTMKAC 2 cut(s) 117, 267
AciI CCGC 6 cut(s) 21, 27, 58, 71, 133, 136
AclWI GGATC 2 cut(s) 395, 408
AcuI CTGAAG 1 cut(s) 464
AcyI GRCGYC 1 cut(s) 114
AfiI CCNNNNNNNGG 4 cut(s) 64, 77, 139, 529
AgsI TTSAA 2 cut(s) 176, 548
AluBI AGCT 6 cut(s) 50, 160, 229, 442, 610, 615
AluI AGCT 6 cut(s) 50, 160, 229, 442, 610, 615
Alw21I GWGCWC 2 cut(s) 52, 612
Alw26I GTCTC 1 cut(s) 481
AlwI GGATC 2 cut(s) 395, 408
AoxI GGCC 2 cut(s) 13, 205
ApeKI GCWGC 2 cut(s) 24, 40
AspLEI GCGC 1 cut(s) 420
AspS9I GGNCC 3 cut(s) 14, 206, 345
AvaII GGWCC 1 cut(s) 345
AxyI CCTNAGG 1 cut(s) 363
BamHI GGATCC 1 cut(s) 400
BanII GRGCYC 2 cut(s) 52, 612
BbsI GAAGAC 1 cut(s) 79
Bbv12I GWGCWC 2 cut(s) 52, 612
BbvI GCAGC 2 cut(s) 27, 36
BccI CCATC 3 cut(s) 82, 466, 527
BcoDI GTCTC 1 cut(s) 481
BfaI CTAG 1 cut(s) 342
BglI GCCNNNNNGGC 1 cut(s) 21
BisI GCNGC 6 cut(s) 22, 25, 28, 41, 71, 136
BlpI GCTNAGC 1 cut(s) 611
BlsI GCNGC 6 cut(s) 23, 26, 29, 42, 72, 137
Bme18I GGWCC 1 cut(s) 345
BmgT120I GGNCC 3 cut(s) 14, 206, 345
BmiI GGNNCC 4 cut(s) 16, 69, 347, 402
BmsI GCATC 2 cut(s) 495, 592
BpiI GAAGAC 1 cut(s) 79
BplI GAGNNNNNCTC 1 cut(s) 599
Bpu1102I GCTNAGC 1 cut(s) 611
BpuEI CTTGAG 1 cut(s) 558
BsaHI GRCGYC 1 cut(s) 114
BsaI GGTCTC 1 cut(s) 481
BsaJI CCNNGG 1 cut(s) 588
Bsc4I CCNNNNNNNGG 4 cut(s) 64, 77, 139, 529
Bse1I ACTGG 1 cut(s) 301
Bse21I CCTNAGG 1 cut(s) 363
BseDI CCNNGG 1 cut(s) 588
BseGI GGATG 4 cut(s) 385, 465, 538, 583
BseLI CCNNNNNNNGG 4 cut(s) 64, 77, 139, 529
BseMII CTCAG 3 cut(s) 139, 221, 354
BseNI ACTGG 1 cut(s) 301
BseXI GCAGC 2 cut(s) 27, 36
BseYI CCCAGC 2 cut(s) 17, 579
Bsh1285I CGRYCG 1 cut(s) 100
BshFI GGCC 2 cut(s) 15, 207
BsiEI CGRYCG 1 cut(s) 100
BsiHKAI GWGCWC 2 cut(s) 52, 612
BslFI GGGAC 2 cut(s) 94, 307
BslI CCNNNNNNNGG 4 cut(s) 64, 77, 139, 529
BsmAI GTCTC 1 cut(s) 481
BsmFI GGGAC 2 cut(s) 94, 307
BsnI GGCC 2 cut(s) 15, 207
Bso31I GGTCTC 1 cut(s) 481
Bsp1286I GDGCHC 2 cut(s) 52, 612
Bsp143I GATC 1 cut(s) 400
Bsp1720I GCTNAGC 1 cut(s) 611
Bsp19I CCATGG 1 cut(s) 588
BspACI CCGC 6 cut(s) 21, 27, 58, 71, 133, 136
BspANI GGCC 2 cut(s) 15, 207
BspCNI CTCAG 3 cut(s) 138, 222, 355
BspLI GGNNCC 4 cut(s) 16, 69, 347, 402
BspPI GGATC 2 cut(s) 395, 408
BspQI GCTCTTC 1 cut(s) 158
BspTNI GGTCTC 1 cut(s) 481
BsrI ACTGG 1 cut(s) 301
BssECI CCNNGG 1 cut(s) 588
BssMI GATC 1 cut(s) 400
BssNI GRCGYC 1 cut(s) 114
BssT1I CCWWGG 1 cut(s) 588
Bst4CI ACNGT 3 cut(s) 85, 98, 184
Bst6I CTCTTC 1 cut(s) 158
BstACI GRCGYC 1 cut(s) 114
BstC8I GCNNGC 1 cut(s) 149
BstDEI CTNAG 4 cut(s) 125, 230, 363, 611
BstDSI CCRYGG 1 cut(s) 588
BstF5I GGATG 4 cut(s) 385, 465, 538, 583
BstHHI GCGC 1 cut(s) 420
BstKTI GATC 1 cut(s) 403
BstMAI GTCTC 1 cut(s) 481
BstMBI GATC 1 cut(s) 400
BstMCI CGRYCG 1 cut(s) 100
BstMWI GCNNNNNNNGC 5 cut(s) 21, 27, 135, 144, 157
BstNSI RCATGY 1 cut(s) 151
BstV1I GCAGC 2 cut(s) 27, 36
BstV2I GAAGAC 1 cut(s) 79
BstX2I RGATCY 1 cut(s) 400
BstXI CCANNNNNNTGG 1 cut(s) 472
BstYI RGATCY 1 cut(s) 400
Bsu36I CCTNAGG 1 cut(s) 363
BsuRI GGCC 2 cut(s) 15, 207
BtgI CCRYGG 1 cut(s) 588
BtsCI GGATG 4 cut(s) 385, 465, 538, 583
Cac8I GCNNGC 1 cut(s) 149
CfoI GCGC 1 cut(s) 420
Cfr13I GGNCC 3 cut(s) 14, 206, 345
CviAII CATG 2 cut(s) 148, 589
DdeI CTNAG 4 cut(s) 125, 230, 363, 611
DpnI GATC 1 cut(s) 402
DpnII GATC 1 cut(s) 400
Eam1104I CTCTTC 1 cut(s) 158
EarI CTCTTC 1 cut(s) 158
EciI GGCGGA 1 cut(s) 122
Ecl136II GAGCTC 2 cut(s) 50, 610
Eco130I CCWWGG 1 cut(s) 588
Eco24I GRGCYC 2 cut(s) 52, 612
Eco31I GGTCTC 1 cut(s) 481
Eco47I GGWCC 1 cut(s) 345
Eco53kI GAGCTC 2 cut(s) 50, 610
Eco57I CTGAAG 1 cut(s) 464
Eco81I CCTNAGG 1 cut(s) 363
EcoICRI GAGCTC 2 cut(s) 50, 610
EcoO109I RGGNCCY 1 cut(s) 345
EcoT14I CCWWGG 1 cut(s) 588
EcoT38I GRGCYC 2 cut(s) 52, 612
ErhI CCWWGG 1 cut(s) 588
FaeI CATG 2 cut(s) 151, 592
FaiI YATR 5 cut(s) 149, 197, 204, 422, 590
FaqI GGGAC 2 cut(s) 94, 307
FatI CATG 2 cut(s) 147, 588
FblI GTMKAC 2 cut(s) 117, 267
Fnu4HI GCNGC 6 cut(s) 22, 25, 28, 41, 71, 136
FokI GGATG 4 cut(s) 392, 472, 545, 570
FriOI GRGCYC 2 cut(s) 52, 612
Fsp4HI GCNGC 6 cut(s) 22, 25, 28, 41, 71, 136
FspBI CTAG 1 cut(s) 342
GlaI GCGC 1 cut(s) 419
GluI GCNGC 6 cut(s) 22, 25, 28, 41, 71, 136
GsaI CCCAGC 2 cut(s) 21, 583
HaeIII GGCC 2 cut(s) 15, 207
HhaI GCGC 1 cut(s) 420
Hin1I GRCGYC 1 cut(s) 114
Hin1II CATG 2 cut(s) 151, 592
Hin6I GCGC 1 cut(s) 418
HinP1I GCGC 1 cut(s) 418
HincII GTYRAC 1 cut(s) 118
HindII GTYRAC 1 cut(s) 118
HinfI GANTC 1 cut(s) 275
Hpy166II GTNNAC 4 cut(s) 36, 118, 247, 268
Hpy188I TCNGA 1 cut(s) 304
Hpy188III TCNNGA 4 cut(s) 101, 110, 499, 605
Hpy8I GTNNAC 4 cut(s) 36, 118, 247, 268
Hpy99I CGWCG 2 cut(s) 119, 122
HpyAV CCTTC 2 cut(s) 170, 419
HpyCH4III ACNGT 3 cut(s) 85, 98, 184
HpyCH4IV ACGT 4 cut(s) 114, 120, 199, 243
HpyCH4V TGCA 3 cut(s) 188, 335, 395
HpyF10VI GCNNNNNNNGC 5 cut(s) 21, 27, 135, 144, 157
HpyF3I CTNAG 4 cut(s) 125, 230, 363, 611
HpySE526I ACGT 4 cut(s) 114, 120, 199, 243
Hsp92I GRCGYC 1 cut(s) 114
Hsp92II CATG 2 cut(s) 151, 592
HspAI GCGC 1 cut(s) 418
Kzo9I GATC 1 cut(s) 400
LguI GCTCTTC 1 cut(s) 158
LmnI GCTCC 1 cut(s) 67
Lsp1109I GCAGC 2 cut(s) 27, 36
LweI GCATC 2 cut(s) 495, 592
MaeI CTAG 1 cut(s) 342
MaeII ACGT 4 cut(s) 114, 120, 199, 243
MaeIII GTNAC 1 cut(s) 233
MalI GATC 1 cut(s) 402
MboI GATC 1 cut(s) 400
MboII GAAGA 3 cut(s) 79, 145, 235
MflI RGATCY 1 cut(s) 400
MhlI GDGCHC 2 cut(s) 52, 612
MluCI AATT 3 cut(s) 212, 355, 540
MmeI TCCRAC 1 cut(s) 357
MnlI CCTC 7 cut(s) 71, 140, 219, 232, 358, 359, 495
MseI TTAA 2 cut(s) 215, 619
MslI CAYNNNNRTG 1 cut(s) 470
MspA1I CMGCKG 2 cut(s) 21, 27
MwoI GCNNNNNNNGC 5 cut(s) 21, 27, 135, 144, 157
NcoI CCATGG 1 cut(s) 588
NdeII GATC 1 cut(s) 400
NlaIII CATG 2 cut(s) 151, 592
NlaIV GGNNCC 4 cut(s) 16, 69, 347, 402
NmuCI GTSAC 1 cut(s) 233
NspI RCATGY 1 cut(s) 151
PaeI GCATGC 1 cut(s) 151
PciSI GCTCTTC 1 cut(s) 158
PfeI GAWTC 1 cut(s) 275
PkrI GCNGC 6 cut(s) 23, 26, 29, 42, 72, 137
PpuMI RGGWCCY 1 cut(s) 345
Psp124BI GAGCTC 2 cut(s) 52, 612
Psp5II RGGWCCY 1 cut(s) 345
PspFI CCCAGC 2 cut(s) 17, 579
PspN4I GGNNCC 4 cut(s) 16, 69, 347, 402
PspPI GGNCC 3 cut(s) 14, 206, 345
PspPPI RGGWCCY 1 cut(s) 345
PsuI RGATCY 1 cut(s) 400
RseI CAYNNNNRTG 1 cut(s) 470
SacI GAGCTC 2 cut(s) 52, 612
SalI GTCGAC 1 cut(s) 116
SapI GCTCTTC 1 cut(s) 158
SaqAI TTAA 2 cut(s) 215, 619
SatI GCNGC 6 cut(s) 22, 25, 28, 41, 71, 136
Sau3AI GATC 1 cut(s) 400
Sau96I GGNCC 3 cut(s) 14, 206, 345
SduI GDGCHC 2 cut(s) 52, 612
SfaNI GCATC 2 cut(s) 495, 592
SgrDI CGTCGACG 1 cut(s) 116
SinI GGWCC 1 cut(s) 345
SmiMI CAYNNNNRTG 1 cut(s) 470
SmlI CTYRAG 2 cut(s) 573, 605
SmoI CTYRAG 2 cut(s) 573, 605
SphI GCATGC 1 cut(s) 151
Sse9I AATT 3 cut(s) 212, 355, 540
SsiI CCGC 6 cut(s) 21, 27, 58, 71, 133, 136
SspMI CTAG 1 cut(s) 342
SstI GAGCTC 2 cut(s) 52, 612
StyI CCWWGG 1 cut(s) 588
TaaI ACNGT 3 cut(s) 85, 98, 184
TaiI ACGT 4 cut(s) 117, 123, 202, 246
TaqI TCGA 2 cut(s) 46, 117
TasI AATT 3 cut(s) 212, 355, 540
TauI GCSGC 4 cut(s) 24, 30, 73, 138
TfiI GAWTC 1 cut(s) 275
Tru1I TTAA 2 cut(s) 215, 619
Tru9I TTAA 2 cut(s) 215, 619
TseFI GTSAC 1 cut(s) 233
TseI GCWGC 2 cut(s) 24, 40
Tsp45I GTSAC 1 cut(s) 233
TspDTI ATGAA 1 cut(s) 267
VpaK11BI GGWCC 1 cut(s) 345
XceI RCATGY 1 cut(s) 151
XmiI GTMKAC 2 cut(s) 117, 267
XspI CTAG 1 cut(s) 342
ZraI GACGTC 1 cut(s) 115
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.