pycom16g21700

Wiskott-Aldrich syndrome protein family member

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0016073
Physical Location & Seq
Forward (+)
20397891 .. 20401431
3541 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 606 bp
ATGTTGTGTGTTGACATAGATCTGACCAGTTGGGTTTGCGTGCAGTCGACAGGACTCCTTCTATCCACCTACTCACAAGTTTCACTCTCTCTCTCACTTAAAGCAAGTCCACTCCCCTTCTCACCCAAAACCCACACAGATCCCTCACTTTCTCTCCCATTTTCCTCCAAACCAAACACAACCCAGATGGCCATCAACAGGGACTCGACACCCCCTCCAATGATCGGCAAGATTGGTCCCTACACTGTGTTCATGACTCCGCCCTCCACCCCTAAGCCGCCTGAACCGGTCTTTGAATCTCCCCGGAAGGTGGCCGCGCCGGTCTTTGAGTCGCCAAAGAAGGTGGCCCCGCCTCCGGTCCAGCCGCCGCCTCAGCAATTGGTGAAGCCCATTGCCGTATCATCGGACTCCTCGGCCGATGGGTCGGTGTTGGGTATTTTTAAGAATGCGGTCACCAAAGTGCAGAAGGCTCATTCAAGTTTGGATGACCATTTGGCGCGTTGGTTTGGGTTAAATCAGTCAAAATATCAATGGGCACTAGATGATTATTACCAGAGCAAGGGGATGATAAGAAATGGACGGTGCAAAACCAAAAGAGATATCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
FAM177

Protein Analysis

202

Amino Acids

21.95

Weight (kDa)

9.47

Isoelectric Point (pI)

49.35

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016073)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G18910
fragaria_vesca FvH4_4g07900
malus_domestica MD13G1249500.v1.1 MD16G1255800.v1.1
prunus_persica Prupe.1G082700_v2.0.a1
pyrus_communis pycom13g21990 pycom16g21700
rosa_chinensis RchiOBHm_Chr4g0401201
rosa_laevigata RLG00000009156
rosa_rugosa Rorug04G0028900
rosa_samantha Rh4AG106400 Rh4BG101100 Rh4CG115400
rosa_wichuraiana Rw4G008610 Rw4G008640

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 47
AccII CGCG 2 cut(s) 317, 499
AciI CCGC 7 cut(s) 260, 278, 315, 350, 365, 368, 449
AclWI GGATC 1 cut(s) 134
AcoI YGGCCR 3 cut(s) 189, 312, 414
AfiI CCNNNNNNNGG 5 cut(s) 198, 224, 310, 355, 559
AgeI ACCGGT 1 cut(s) 286
AgsI TTSAA 2 cut(s) 296, 477
AleI CACNNNNGTG 1 cut(s) 458
AlwI GGATC 1 cut(s) 134
AoxI GGCC 4 cut(s) 189, 312, 345, 414
AsiGI ACCGGT 1 cut(s) 286
AspLEI GCGC 2 cut(s) 319, 499
AspS9I GGNCC 3 cut(s) 236, 346, 358
AsuC2I CCSGG 1 cut(s) 304
AsuHPI GGTGA 3 cut(s) 114, 394, 445
AvaII GGWCC 2 cut(s) 236, 358
BaeGI GKGCMC 1 cut(s) 538
BalI TGGCCA 1 cut(s) 191
BbvCI CCTCAGC 1 cut(s) 372
BccI CCATC 3 cut(s) 181, 200, 413
BceAI ACGGC 1 cut(s) 380
BcnI CCSGG 1 cut(s) 304
BfaI CTAG 2 cut(s) 539, 604
BglII AGATCT 1 cut(s) 19
BisI GCNGC 4 cut(s) 278, 315, 365, 368
BlsI GCNGC 4 cut(s) 279, 316, 366, 369
Bme1390I CCNGG 1 cut(s) 304
Bme18I GGWCC 2 cut(s) 236, 358
BmgT120I GGNCC 3 cut(s) 236, 346, 358
BmiI GGNNCC 2 cut(s) 238, 348
BmrFI CCNGG 1 cut(s) 304
Bpu10I CCTNAGC 2 cut(s) 273, 372
BpuMI CCSGG 1 cut(s) 304
BsaBI GATNNNNATC 1 cut(s) 191
BsaJI CCNNGG 2 cut(s) 302, 411
BsaWI WCCGGW 2 cut(s) 286, 355
BsaXI ACNNNNNCTCC 4 cut(s) 138, 168, 199, 229
Bsc4I CCNNNNNNNGG 5 cut(s) 198, 224, 310, 355, 559
Bse118I RCCGGY 2 cut(s) 286, 319
Bse1I ACTGG 1 cut(s) 27
Bse3DI GCAATG 1 cut(s) 390
Bse8I GATNNNNATC 1 cut(s) 191
BseDI CCNNGG 2 cut(s) 302, 411
BseGI GGATG 2 cut(s) 490, 570
BseJI GATNNNNATC 1 cut(s) 191
BseLI CCNNNNNNNGG 5 cut(s) 198, 224, 310, 355, 559
BseMI GCAATG 1 cut(s) 390
BseMII CTCAG 1 cut(s) 386
BseNI ACTGG 1 cut(s) 27
BseRI GAGGAG 1 cut(s) 400
BseSI GKGCMC 1 cut(s) 538
BseX3I CGGCCG 1 cut(s) 414
BsgI GTGCAG 2 cut(s) 62, 482
Bsh1236I CGCG 2 cut(s) 317, 499
Bsh1285I CGRYCG 1 cut(s) 417
BshFI GGCC 4 cut(s) 191, 314, 347, 416
BshTI ACCGGT 1 cut(s) 286
BsiEI CGRYCG 1 cut(s) 417
BsiSI CCGG 4 cut(s) 287, 304, 320, 356
BslFI GGGAC 2 cut(s) 215, 222
BslI CCNNNNNNNGG 5 cut(s) 198, 224, 310, 355, 559
BsmFI GGGAC 2 cut(s) 215, 222
BsmI GAATGC 1 cut(s) 451
BsnI GGCC 4 cut(s) 191, 314, 347, 416
Bsp1286I GDGCHC 1 cut(s) 538
Bsp143I GATC 3 cut(s) 19, 139, 222
BspACI CCGC 7 cut(s) 260, 278, 315, 350, 365, 368, 449
BspANI GGCC 4 cut(s) 191, 314, 347, 416
BspCNI CTCAG 1 cut(s) 385
BspFNI CGCG 2 cut(s) 317, 499
BspHI TCATGA 1 cut(s) 252
BspLI GGNNCC 2 cut(s) 238, 348
BspPI GGATC 1 cut(s) 134
BsrDI GCAATG 1 cut(s) 390
BsrFI RCCGGY 2 cut(s) 286, 319
BsrI ACTGG 1 cut(s) 27
BssAI RCCGGY 2 cut(s) 286, 319
BssECI CCNNGG 2 cut(s) 302, 411
BssMI GATC 3 cut(s) 19, 139, 222
Bst4CI ACNGT 2 cut(s) 247, 582
BstC8I GCNNGC 1 cut(s) 41
BstDEI CTNAG 2 cut(s) 273, 372
BstEII GGTNACC 1 cut(s) 451
BstF5I GGATG 2 cut(s) 490, 570
BstFNI CGCG 2 cut(s) 317, 499
BstHHI GCGC 2 cut(s) 319, 499
BstKTI GATC 3 cut(s) 22, 142, 225
BstMBI GATC 3 cut(s) 19, 139, 222
BstMCI CGRYCG 1 cut(s) 417
BstMWI GCNNNNNNNGC 1 cut(s) 373
BstPI GGTNACC 1 cut(s) 451
BstSCI CCNGG 1 cut(s) 302
BstSLI GKGCMC 1 cut(s) 538
BstUI CGCG 2 cut(s) 317, 499
BstX2I RGATCY 2 cut(s) 19, 139
BstYI RGATCY 2 cut(s) 19, 139
BstZI CGGCCG 1 cut(s) 414
BsuRI GGCC 4 cut(s) 191, 314, 347, 416
BtsCI GGATG 2 cut(s) 490, 570
BtsIMutI CAGTG 1 cut(s) 243
Cac8I GCNNGC 1 cut(s) 41
CciI TCATGA 1 cut(s) 252
CfoI GCGC 2 cut(s) 319, 499
Cfr10I RCCGGY 2 cut(s) 286, 319
Cfr13I GGNCC 3 cut(s) 236, 346, 358
CspAI ACCGGT 1 cut(s) 286
CspCI CAANNNNNGTGG 2 cut(s) 324, 359
CviAII CATG 1 cut(s) 253
CviJI RGCY 8 cut(s) 191, 277, 314, 347, 364, 388, 416, 470
CviKI_1 RGCY 8 cut(s) 191, 277, 314, 347, 364, 388, 416, 470
DdeI CTNAG 2 cut(s) 273, 372
DpnI GATC 3 cut(s) 21, 141, 224
DpnII GATC 3 cut(s) 19, 139, 222
EaeI YGGCCR 3 cut(s) 189, 312, 414
EagI CGGCCG 1 cut(s) 414
EciI GGCGGA 1 cut(s) 249
EclXI CGGCCG 1 cut(s) 414
Eco32I GATATC 1 cut(s) 601
Eco47I GGWCC 2 cut(s) 236, 358
Eco52I CGGCCG 1 cut(s) 414
Eco91I GGTNACC 1 cut(s) 451
EcoO65I GGTNACC 1 cut(s) 451
EcoRV GATATC 1 cut(s) 601
FaeI CATG 1 cut(s) 256
FaiI YATR 2 cut(s) 17, 254
FaqI GGGAC 2 cut(s) 215, 222
FatI CATG 1 cut(s) 252
FauI CCCGC 1 cut(s) 357
FblI GTMKAC 1 cut(s) 47
Fnu4HI GCNGC 4 cut(s) 278, 315, 365, 368
FokI GGATG 2 cut(s) 497, 577
Fsp4HI GCNGC 4 cut(s) 278, 315, 365, 368
FspBI CTAG 2 cut(s) 539, 604
GlaI GCGC 2 cut(s) 318, 498
GluI GCNGC 4 cut(s) 278, 315, 365, 368
HaeIII GGCC 4 cut(s) 191, 314, 347, 416
HapII CCGG 4 cut(s) 287, 304, 320, 356
HhaI GCGC 2 cut(s) 319, 499
Hin1II CATG 1 cut(s) 256
Hin6I GCGC 2 cut(s) 317, 497
HinP1I GCGC 2 cut(s) 317, 497
HincII GTYRAC 2 cut(s) 13, 48
HindII GTYRAC 2 cut(s) 13, 48
HinfI GANTC 6 cut(s) 54, 203, 256, 296, 329, 407
HpaII CCGG 4 cut(s) 287, 304, 320, 356
HphI GGTGA 3 cut(s) 114, 394, 445
Hpy166II GTNNAC 3 cut(s) 13, 48, 110
Hpy188I TCNGA 2 cut(s) 24, 406
Hpy188III TCNNGA 1 cut(s) 253
Hpy8I GTNNAC 3 cut(s) 13, 48, 110
HpyAV CCTTC 5 cut(s) 68, 127, 301, 334, 460
HpyCH4III ACNGT 2 cut(s) 247, 582
HpyCH4V TGCA 3 cut(s) 43, 463, 585
HpyF10VI GCNNNNNNNGC 1 cut(s) 373
HpyF3I CTNAG 2 cut(s) 273, 372
Hsp92II CATG 1 cut(s) 256
HspAI GCGC 2 cut(s) 317, 497
Kzo9I GATC 3 cut(s) 19, 139, 222
MaeI CTAG 2 cut(s) 539, 604
MaeIII GTNAC 1 cut(s) 451
MalI GATC 3 cut(s) 21, 141, 224
MboI GATC 3 cut(s) 19, 139, 222
MfeI CAATTG 1 cut(s) 377
MflI RGATCY 2 cut(s) 19, 139
MhlI GDGCHC 1 cut(s) 538
MlsI TGGCCA 1 cut(s) 191
MluCI AATT 1 cut(s) 377
MluNI TGGCCA 1 cut(s) 191
MlyI GAGTC 5 cut(s) 48, 197, 250, 338, 401
MnlI CCTC 7 cut(s) 154, 175, 225, 274, 363, 381, 421
Mox20I TGGCCA 1 cut(s) 191
MscI TGGCCA 1 cut(s) 191
MseI TTAA 3 cut(s) 99, 441, 512
MslI CAYNNNNRTG 1 cut(s) 458
Msp20I TGGCCA 1 cut(s) 191
MspI CCGG 4 cut(s) 287, 304, 320, 356
MspR9I CCNGG 1 cut(s) 304
MunI CAATTG 1 cut(s) 377
Mva1269I GAATGC 1 cut(s) 451
MvnI CGCG 2 cut(s) 317, 499
MwoI GCNNNNNNNGC 1 cut(s) 373
NciI CCSGG 1 cut(s) 304
NdeII GATC 3 cut(s) 19, 139, 222
NlaIII CATG 1 cut(s) 256
NlaIV GGNNCC 2 cut(s) 238, 348
NmeAIII GCCGAG 1 cut(s) 392
NmuCI GTSAC 1 cut(s) 451
OliI CACNNNNGTG 1 cut(s) 458
PagI TCATGA 1 cut(s) 252
PctI GAATGC 1 cut(s) 451
PfeI GAWTC 1 cut(s) 296
PinAI ACCGGT 1 cut(s) 286
PkrI GCNGC 4 cut(s) 279, 316, 366, 369
PleI GAGTC 5 cut(s) 48, 197, 250, 337, 401
PpsI GAGTC 5 cut(s) 48, 197, 250, 337, 401
PspEI GGTNACC 1 cut(s) 451
PspN4I GGNNCC 2 cut(s) 238, 348
PspPI GGNCC 3 cut(s) 236, 346, 358
PsuI RGATCY 2 cut(s) 19, 139
RseI CAYNNNNRTG 1 cut(s) 458
SalI GTCGAC 1 cut(s) 46
SaqAI TTAA 3 cut(s) 99, 441, 512
SatI GCNGC 4 cut(s) 278, 315, 365, 368
Sau3AI GATC 3 cut(s) 19, 139, 222
Sau96I GGNCC 3 cut(s) 236, 346, 358
SchI GAGTC 5 cut(s) 48, 197, 250, 338, 401
ScrFI CCNGG 1 cut(s) 304
SduI GDGCHC 1 cut(s) 538
SetI ASST 3 cut(s) 71, 312, 345
SinI GGWCC 2 cut(s) 236, 358
SmiMI CAYNNNNRTG 1 cut(s) 458
Sse9I AATT 1 cut(s) 377
SsiI CCGC 7 cut(s) 260, 278, 315, 350, 365, 368, 449
SspMI CTAG 2 cut(s) 539, 604
StyD4I CCNGG 1 cut(s) 302
TaaI ACNGT 2 cut(s) 247, 582
TaqI TCGA 2 cut(s) 47, 206
TasI AATT 1 cut(s) 377
TauI GCSGC 4 cut(s) 280, 317, 367, 370
TfiI GAWTC 1 cut(s) 296
Tru1I TTAA 3 cut(s) 99, 441, 512
Tru9I TTAA 3 cut(s) 99, 441, 512
TscAI CASTG 1 cut(s) 250
TseFI GTSAC 1 cut(s) 451
Tsp45I GTSAC 1 cut(s) 451
TspDTI ATGAA 1 cut(s) 241
TspRI CASTG 1 cut(s) 250
VpaK11BI GGWCC 2 cut(s) 236, 358
XmiI GTMKAC 1 cut(s) 47
XspI CTAG 2 cut(s) 539, 604
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.