pycom16g22600

Translation initiation factor eIF3 subunit 135

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0011973
Physical Location & Seq
Forward (+)
22681771 .. 22705646
23876 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 5565 bp
ATGGCTCCCAAGACTGGCAAGGCCAAGCCCCATAAACCCAAGGGAGACAAGAAGAAAAAGGAGGAGAAAGTTTTGCCTACCGTAATAGAGATTAGCATCGAAACACCAGATGAGTTACAAGTAACTCTCAAGGGCATCTCTACGGACAGGATCCTGGACGTGAGAAAGCTGTTGGGAGTCCACGTGGAGACATGTCATTTGACCAATTTCTCCTTATCACACGAGGTGCGAGGACCCAAACTCAAAGATTCGGTCGACATCCTCTCCCTCAAACCCTGCCACCTCACCATCGTCGAAGAGGACTACACAGAAGAGCAAGCCGTGGCACACATCCGGCGACTGATGGACATCGTCGCTTGCACGACATCTTTTGGTTCGTCATCCGCTTCTTCACCTAAAACGCCTGGCCGATCAAACTCAAAGGGCGACGTACCGCAGCACCAAAACGACGACGGGCCCAATGCAGATGCAAGGGCCAAAGTTTCGGGTTCAATTTCGATCGTTGATGCCGAGTCAGCTGCTGCCTCAACCGTGTCAATGTATCCTCCTCCAAAGCTCGGGCAGTTCTACGACTTCTTTTCCTTGTCTCACCTTACGCCTCCTCTTCACTACATAAGAAGATCTACGCGTCCGTTCCTTGAGGATAAGACAGAAGACGACTTGTTTCAAATGGACATTCGGGTTTGTAGCGGGAAGCCGACGACAATCGTGGCTTCCAGGAAAGGATTCTACCCTGCTGGAAAAAGAGCTCTAATAGTTCACTCTTTGGTTGTTCTTCTTCAGCAAATTAGTAGGCCATTTGATGTTGCATACAACGCTGTTATGAAGGCTTTCATTGAGCACAATAGATTTGGAAACCTTCCCTACGGTTTTCGCGCAAACACATGGGTTGTCCCTCCAATTGCTGCTGATAAACCGTCAGTTTTCCCATCACTTCCTTTGGAAGACGAAAACTGGGGAGGAGGCGGAGGTGGTCAGGGAAGAGACGGTAAACATGATTACAGGCCATGGGCAAAGGAGTTTGCCATTCTAAAAGTAATGCCTTGCTCAACATCAGAGGAGAGGCAAGTGCGAGACCGCAAGGCTTTTTTGCTTCACAGTTTATTTGTGGACGTCTCTGTTCTTAAAGCTGTTACAGCAATTAAGCGTCTAGTTGAGACTAACCATTGTGCCTTAAATGATCCCACTTTTTCACTTGTTCACGAGGAAAGTGTTGGAGATTTAATCATTAAGGTGACCAGAAATATGCCCGATGCAAGTATAAAGGTAGACTGTAAAAAAGATGGCAGCCAAGTTCTTGGAATTCCGCAAGAACAAGTTACTCAGAGAAACCTACTCAAAGGAATAACAGCTGACGAAAGTGCAACTGTTCATGATACTGCTACACTAGGTGTTGTGGTTGTCAGACTTTGTGGGTTTACGGCTGTTGTGAGGGTTTCAAGTGAGGTGAATTGGGAAGGAAAACCTGTTCCTAAGGACGTTGAAATTGAGGACCAGCTTGAAGGTGGTGCTAATGCTTTGAATGTTAATAGCTTGAGGCTGTTATTGCAACAGTCCTCCCTGCCTCAATCATCAAATGCGGTTATACGTACAGATTTTGAAAGTATACTTGCTACTAGATCTCTCGTGAAGAAAGTACTTGAAGACAGTCTGTTAAGATTGCAGGATGGACCCACTAATAATACTAAGTCCATTAGATGGGAGCTAGGAGCATGTTGGGTACAACATTTGCAAAATCAAGCTTCGGGAAAAACTGAATCTAAAAAAACTGAACAAGCTAAGATTGAGCCAGTTGTTAAGGGTCTTGGAAAGCAAGGGGGGTTACTAAAGGAAATTAAAAAGAAAATGGATGTCAGGAGCAACAAAACTGACCAGCTGAAGGATGTTGTTGGGGTTAACAACCTTGATATGAATCAGAATTTTGATACCACTACTCAGGAGGAATTAGAAAAGCGAGATGTAGAAAAGGAAACTATTTGGAGAAAGATGCTTCCTGAATCGTCATATTTGCGCCTCAAAGAATCAGAAACTGGTCTTCACCTTCAGTCACCCAATGAGTTGATTGAAATGGCACATAAATACTATGTTGACACTGCACTTCCAAAACTGGTTGCAGATTTTGGCTCCCTTGAACTTTCACCAGTCGATGGAAGGACATTGACAGATTTTATGCATACTAGGGGTTTGCAAATGTCTTCCCTAGGACGTGTGGTTGAACTTGCAGATAAGCTTCCTCATGTACAATCACTTTGTATCCATGAGATGGTTGTGCGAGCCTACAAACACATACTCCAAGCAGTTGTAGCAGCAGTTGATAATATTGCAGACTTGGCTGCATCAATAGCGGCATGTTTAAATATATTGCTAGGAACACCATCAACTGAAAATGGGGATGTAGATATTACTTATGATGAAGCATTAAAATGGAAATGGGTGGAAAGTTTCCTTTTGAAGAGGTTTGGGTGGCAATGGAAACACGAAACTGTTAAGGATCTAAGGAAGTTTGCCATTCTCCGTGGATTGTCCCACAAGGTTGGACTTGAGCTTGTTCCTAGGGACTACGATATGGACAATGTATCTCCTTTTCGGAAATCAGATATAGTAAGCATGGTCCCTGTATATAAGCACGTTGCATGTTCATCCGCGGATGGGCGTACACTCTTAGAATCGTCCAAGACTTTGTTGGATAAAGGTAAACTGGAGGAAGCGGTTAATTTTGGCACTAAGGCACTCTCAAAACTAGTGTCAGTATGTGGGCCTTATCATCGAATGACAGCGGGAGCATATAGTCTTCTTGCTGTAGTATTGTACCACACTGGGGATTTCAACCAGGCGACCATCTATCAGCAAAAGGCATTGGATATCAACGAGAGGGAGCTTGGACTTGATCATCCTGATACGATGAAAAGTTATGGGGATTTGGCTGTTTTCTATTACAGACTTCAACATACAGAGTTGGCCCTCAAGTACGTCAACCGTGCTTTGTATCTTTTGCATCTAACATGTGGGCCGTCTCATCCAAATACGGCTGCCACCTATATTAACGTGGCAATGATGGAAGAAGGTTTGGGGAATGTCCATGTTGCTCTTAGGTATCTCCATGAAGCTCTAAAGTGCAATCAAAGGCTACTTGGACCTGATCATATACAGACCGCTGCTAGCTATCATGCCATTGCTATAGCACTCTCTTTGATGGAAGCTTACACATTAAGTGTTCAGCATGAACAAACTACCTTACAGATACTTCAAGCAAAACTTGGCTCTGAGGATCTACGCACACAGGATGCGGCTGCATGGCTTGAGTATTTCGAGTCCAAGGCTTTGGAGCAGCAAGAAGCTGCACGCAATGGTACTCCAAAGCCAGATGCCTCTATCTCCAGCAAAGGACATTTAAGTGTGTCAGACCTTTTGGATTATATAACACCAGATGCTGATACGAAAGCGAGGGATGCAAGAAAAGCTCGTGCAAAGGTTAAAGGAAAACCAGGAAAAAACGAGGCAATAGTCTCAGATGGATACCAGAAGGATGAAATTTTGTTACCAAGTCAGCTTGTTGCCGAGAATTCAGGTGATAAAGAGAACCAGTCTGCACCTCCATTTGCAGAACCTAAGCATGAGAAAGTTGATTCAAGTCTACTGGATCAATCAATAATATTCGAAAAAAAAGATGATGCAGCACAAGATGACGCCTCAGATGAAGGTTGGCACGAAGCAGTTCCTAAAGGTCGTTCACCCATGGGTCGCAAATCCTTTCTGTCAAGGAGACCAAGCCTCGAAAAATTGAATACTAATTTTGTGCATGCATCCCAATTGTCACGATACCATGGAAAGCCTAATAGTTTGACATCTCAAAAAACAAGCCCAATTGAGCCTGCTGCTTTTCCTGGACCTGCTCTTCCCGTCTCAAAAAAGTTTGTTAAGAGTGCAAGCTTCAGTCCTAAGCCAAGCAACTCTAGCATGTCAGTTAGTGGAGGGACAGAGAAGCTCTCAAACCCAAAGTCAGCACCTTCTACTCCAGCTTCTATTAATAAAGTTACCAAGTCATCTTCTGTGACAAATCCAACCAGTGTTCAGTCAGCTGGAAAACTTTTCTCTTATAAAGAAGTTGCGTTGGCTCCTCCTGGAACAATTGTGAAGGCAGTGGCAGAGAAATTACCAAAAGGAAATGTTCCTATTGTACCACTTTCTAAGGTTGGCCAGGAGAAAACTGCAACAGATGTTACCGGTGAAGTGATACTAGTTAAAGATGTCGAGGAAGAGGAAAAGCAAAAACTTGTGGGAGAGAAAGTGGATTTGGCTCTTGAGGAAAATAGTATTGATGTAAAACATACAAAAGTGGAGAACTCTGTGGCAAAGAAATCTCTGGGAGTTGTAAAGCATGCTAGTGCTGGAGTACAAGTGGAAACTGAGATCATAGAAATGAAAACCACTGCTTATGAAGAAGGACAAGTTGAAAATGCTGAAGTCATGGTTTCAAACGTTGAGATTTCTATTACTTCCCAACCCCCAAACGCTACTCAAAATGGACAATTGCAAACTCAAGCGCTGGACAGTTCGACCAAGATTTGCCCTGATTCAGAACCTTCATTTGTTTTAACTGAAAGCACAACTCAATTTATGGAAGAAAATTCTTCCCTTTCGGAAGCAAAGGTTGAAGAAGATGGAAGTCCACATGATTTCTCTGATGGTGGGATAGTTAAGCAAGGGCCAACTGATGGAGAAAAGTTAGATGAACCAGAAACTAGAAAAGAGACCACCAAGAAACTTTCTGCAGCCGCACCACCATTCAATCCATCCCTAATACCAGTTTTTGGTTCAGTTCCAGTGGCAGGTTCCAAAGATCATGGAGGGATTTTGCCCCCACCAGTAAATATTCCTCCTATGCTTACGATCAGTTCTGTTCGTAGATCACCTCATCAGTCAGCAACAGCAAGGGTTCCGTATGGTCCACGATTGGCTGGTGGATATAATAGATCTGGAAGTCGGGCTTCACGTAATAAACATGTCTTCCACAATGGTGAGAACACGGGGGATGGAAATCACTTTAGTCCACCTAGAGTCATGAACCCACATGCGGCAGAGTTTGTACCTGGCCAACCTTGGATTCCCAATGGCTATCCAGTATCTCCTAGTGATTTTTTGCCTTCCCCAAGTGGTTATCCCATGTCCCCAAATGGATATCCATTGTCGCCTAATGATATTCCAGTGACTCAAAATGCGTTCCCAACATCTCCAATTGGTTCAGAAGAGTCGTTAACAATTATAAATGATCCACTTGGTGTTGAAACAAATATTAAAGGTGACGCAAAAGAGAATAATGACATGTCTTCATTAGAAGTTGGATGCGAGATGCAAAAAATTGAAGAAGAGTTGCAGAAAGAAGAGTATGTTGACAATGTAGTCTCGCATTCTAATCTTGAAGAAAAGCCTATTGAAACATATACGGTTGTTGATATTGATGTAGCAAAAGAGATCAGCAGCAAATTAGTGGTTGAGGAAAATCCAAGTAAATGTTGGGGAGATTATAGTGACAATGAAGCTGAGGTCATTCAAGTTTCTAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001101 GO:0008150 GO:0009719 GO:0009725 GO:0009737 GO:0010033 GO:0033993 GO:0042221 GO:0050896 GO:0097305 GO:1901700
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CLU CLU_N TPR_12 eIF3_p135

Protein Analysis

1855

Amino Acids

203.11

Weight (kDa)

6.0

Isoelectric Point (pI)

46.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CLU_N PF15044 48 - 118 1.2e-09 Mitochondrial function, CLU-N-term
eIF3_p135 PF12807 712 - 855 1.3e-22 Translation initiation factor eIF3 subunit 135
TPR_12 PF13424 893 - 956 5.8e-07 Tetratricopeptide repeat
TPR_NPHP3 PF24885 895 - 995 2.3e-07 Nephrocystin-3 TPR domain
TPR_12 PF13424 925 - 995 1.5e-12 Tetratricopeptide repeat
TPR_10 PF13374 925 - 966 2.1e-09 Tetratricopeptide repeat
TPR_12 PF13424 1009 - 1083 2.4e-11 Tetratricopeptide repeat
TPR_10 PF13374 1009 - 1049 1.1e-07 Tetratricopeptide repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011973)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G28080
fragaria_vesca FvH4_3g24640 FvH4_3g24640 FvH4_3g24640
malus_domestica MD16G1258600.v1.1
prunus_persica Prupe.1G101800_v2.0.a1
pyrus_communis pycom16g22600
rosa_chinensis RchiOBHm_Chr4g0405441
rosa_laevigata RLG00000008842
rosa_multiflora Rmu_co8255623.1_g000001 Rmu_sc0000575.1_g000017 Rmu_sc0000575.1_g000018
rosa_roxburghii Rroxscaffold_5G00349900
rosa_rugosa Rorug04G0060200
rosa_samantha Rh4AG131900 Rh4BG126800 Rh4CG139300 Rh4DG125900
rosa_wichuraiana Rw4G010680

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 4098, 5264
AasI GACNNNNNNGTC 1 cut(s) 5399
AatII GACGTC 1 cut(s) 1116
Acc36I ACCTGC 2 cut(s) 3898, 4820
AccB7I CCANNNNNTGG 5 cut(s) 1698, 2147, 2263, 4715, 4811
AccI GTMKAC 4 cut(s) 255, 1269, 1606, 3634
AccII CGCG 3 cut(s) 628, 876, 2645
AclI AACGTT 1 cut(s) 4479
AclWI GGATC 7 cut(s) 145, 158, 1175, 2499, 3276, 3648, 5264
AcoI YGGCCR 3 cut(s) 406, 4195, 5092
AcsI RAATTY 5 cut(s) 1302, 1918, 3531, 3562, 4627
AcuI CTGAAG 5 cut(s) 764, 1898, 2027, 3916, 4482
AcvI CACGTG 1 cut(s) 184
AcyI GRCGYC 2 cut(s) 1113, 3687
AdeI CACNNNGTG 3 cut(s) 226, 1391, 5279
AfeI AGCGCT 1 cut(s) 4545
AflIII ACRYGT 6 cut(s) 191, 626, 2206, 3002, 5002, 5322
AgeI ACCGGT 1 cut(s) 4223
AhlI ACTAGT 2 cut(s) 2740, 4237
AjiI CACGTC 2 cut(s) 160, 2207
AjnI CCWGG 9 cut(s) 153, 403, 716, 2829, 3484, 3883, 4120, 4197, 5089
AjuI GAANNNNNNNTTGG 4 cut(s) 4277, 4309, 5088, 5120
AleI CACNNNNGTG 1 cut(s) 5016
AloI GAACNNNNNNTCC 2 cut(s) 1452, 1484
Alw21I GWGCWC 2 cut(s) 751, 843
AlwI GGATC 7 cut(s) 145, 158, 1175, 2499, 3276, 3648, 5264
AlwNI CAGNNNCTG 3 cut(s) 521, 2030, 3431
Ama87I CYCGRG 1 cut(s) 557
Aor51HI AGCGCT 1 cut(s) 4545
ApaI GGGCCC 1 cut(s) 459
ApoI RAATTY 5 cut(s) 1302, 1918, 3531, 3562, 4627
ArsI GACNNNNNNTTYG 8 cut(s) 181, 213, 237, 269, 2729, 2761, 3983, 4015
AseI ATTAAT 1 cut(s) 4026
AsiGI ACCGGT 1 cut(s) 4223
Asp700I GAANNNNTTC 3 cut(s) 725, 830, 3714
AspA2I CCTAGG 2 cut(s) 2200, 2552
AspLEI GCGC 3 cut(s) 878, 2013, 4546
AsuII TTCGAA 1 cut(s) 3657
AsuNHI GCTAGC 1 cut(s) 3158
AvaI CYCGRG 1 cut(s) 557
AvaII GGWCC 7 cut(s) 233, 1492, 1670, 2611, 3134, 3887, 4946
AvrII CCTAGG 2 cut(s) 2200, 2552
AxyI CCTNAGG 1 cut(s) 1473
BaeGI GKGCMC 1 cut(s) 459
BalI TGGCCA 2 cut(s) 4197, 5094
BamHI GGATCC 1 cut(s) 150
BanII GRGCYC 2 cut(s) 459, 751
BauI CACGAG 4 cut(s) 221, 1202, 1625, 3462
BbrPI CACGTG 1 cut(s) 184
BbsI GAAGAC 8 cut(s) 660, 951, 1650, 2027, 2187, 2783, 4999, 5319
Bbv12I GWGCWC 2 cut(s) 751, 843
BbvCI CCTCAGC 1 cut(s) 5541
BceAI ACGGC 4 cut(s) 305, 1437, 2995, 3042
BcgI CGANNNNNNTGC 2 cut(s) 500, 534
BciT130I CCWGG 9 cut(s) 155, 405, 718, 2831, 3486, 3885, 4122, 4199, 5091
BciVI GTATCC 3 cut(s) 552, 2264, 3509
BclI TGATCA 2 cut(s) 2887, 3139
BcuI ACTAGT 2 cut(s) 2740, 4237
BfmI CTRYAG 3 cut(s) 2799, 3177, 4770
BfoI RGCGCY 1 cut(s) 4547
BfuAI ACCTGC 2 cut(s) 3898, 4820
BfuI GTATCC 3 cut(s) 552, 2264, 3509
BglII AGATCT 3 cut(s) 620, 1619, 4973
BlnI CCTAGG 2 cut(s) 2200, 2552
BmcAI AGTACT 1 cut(s) 1638
Bme1390I CCNGG 9 cut(s) 155, 405, 718, 2831, 3486, 3885, 4122, 4199, 5091
Bme18I GGWCC 7 cut(s) 233, 1492, 1670, 2611, 3134, 3887, 4946
BmeT110I CYCGRG 1 cut(s) 557
BmgBI CACGTC 2 cut(s) 160, 2207
BmrFI CCNGG 9 cut(s) 155, 405, 718, 2831, 3486, 3885, 4122, 4199, 5091
BmrI ACTGGG 2 cut(s) 964, 2826
BmtI GCTAGC 1 cut(s) 3162
BmuI ACTGGG 2 cut(s) 964, 2826
BpiI GAAGAC 8 cut(s) 660, 951, 1650, 2027, 2187, 2783, 4999, 5319
BplI GAGNNNNNCTC 2 cut(s) 3971, 4003
BpmI CTGGAG 4 cut(s) 2720, 3361, 3999, 4410
Bpu10I CCTNAGC 3 cut(s) 3609, 3939, 5541
Bpu14I TTCGAA 1 cut(s) 3657
BpuEI CTTGAG 8 cut(s) 113, 659, 1555, 2561, 2948, 3320, 4322, 4524
BsaAI YACGTR 3 cut(s) 184, 1589, 4994
BsaBI GATNNNNATC 4 cut(s) 95, 347, 1911, 5037
BsaHI GRCGYC 2 cut(s) 1113, 3687
BsaI GGTCTC 3 cut(s) 1068, 3757, 4745
BsaWI WCCGGW 1 cut(s) 4223
Bse118I RCCGGY 1 cut(s) 4223
Bse21I CCTNAGG 1 cut(s) 1473
Bse3DI GCAATG 4 cut(s) 2474, 3057, 3171, 3352
Bse8I GATNNNNATC 4 cut(s) 95, 347, 1911, 5037
BseBI CCWGG 9 cut(s) 155, 405, 718, 2831, 3486, 3885, 4122, 4199, 5091
BseJI GATNNNNATC 4 cut(s) 95, 347, 1911, 5037
BseMI GCAATG 4 cut(s) 2474, 3057, 3171, 3352
BseMII CTCAG 7 cut(s) 1337, 1949, 3255, 3522, 3705, 4398, 5532
BseRI GAGGAG 6 cut(s) 77, 537, 591, 975, 1073, 4107
BseSI GKGCMC 1 cut(s) 459
BsgI GTGCAG 3 cut(s) 2079, 3324, 3573
Bsh1236I CGCG 3 cut(s) 628, 876, 2645
Bsh1285I CGRYCG 2 cut(s) 255, 501
BshTI ACCGGT 1 cut(s) 4223
BsiEI CGRYCG 2 cut(s) 255, 501
BsiHKAI GWGCWC 2 cut(s) 751, 843
BsiHKCI CYCGRG 1 cut(s) 557
BsiSI CCGG 2 cut(s) 334, 4224
BslFI GGGAC 6 cut(s) 878, 2509, 2570, 2597, 3988, 5152
BsmBI CGTCTC 4 cut(s) 978, 1120, 3018, 3907
BsmFI GGGAC 6 cut(s) 878, 2509, 2570, 2597, 3988, 5152
BsmI GAATGC 1 cut(s) 5407
Bso31I GGTCTC 3 cut(s) 1068, 3757, 4745
BsoBI CYCGRG 1 cut(s) 557
Bsp119I TTCGAA 1 cut(s) 3657
Bsp120I GGGCCC 1 cut(s) 455
Bsp1286I GDGCHC 3 cut(s) 459, 751, 843
Bsp1407I TGTACA 1 cut(s) 2239
Bsp19I CCATGG 3 cut(s) 1007, 3735, 3823
BspCNI CTCAG 7 cut(s) 1336, 1948, 3256, 3521, 3704, 4399, 5533
BspFNI CGCG 3 cut(s) 628, 876, 2645
BspHI TCATGA 2 cut(s) 1372, 5061
BspMAI CTGCAG 1 cut(s) 4774
BspMI ACCTGC 2 cut(s) 3898, 4820
BspOI GCTAGC 1 cut(s) 3162
BspPI GGATC 7 cut(s) 145, 158, 1175, 2499, 3276, 3648, 5264
BspQI GCTCTTC 2 cut(s) 306, 3900
BspT104I TTCGAA 1 cut(s) 3657
BspTNI GGTCTC 3 cut(s) 1068, 3757, 4745
BsrDI GCAATG 4 cut(s) 2474, 3057, 3171, 3352
BsrFI RCCGGY 1 cut(s) 4223
BsrGI TGTACA 1 cut(s) 2239
BssAI RCCGGY 1 cut(s) 4223
BssNAI GTATAC 1 cut(s) 1607
BssNI GRCGYC 2 cut(s) 1113, 3687
BssSI CACGAG 4 cut(s) 221, 1202, 1625, 3462
BssT1I CCWWGG 8 cut(s) 39, 1007, 2200, 2552, 3315, 3735, 3823, 5099
Bst1107I GTATAC 1 cut(s) 1607
Bst2BI CACGAG 4 cut(s) 221, 1202, 1625, 3462
Bst2UI CCWGG 9 cut(s) 155, 405, 718, 2831, 3486, 3885, 4122, 4199, 5091
BstACI GRCGYC 2 cut(s) 1113, 3687
BstAUI TGTACA 1 cut(s) 2239
BstBAI YACGTR 3 cut(s) 184, 1589, 4994
BstBI TTCGAA 1 cut(s) 3657
BstC8I GCNNGC 9 cut(s) 318, 358, 2274, 3160, 3343, 3801, 3873, 3928, 4380
BstDSI CCRYGG 6 cut(s) 321, 1007, 2515, 2643, 3735, 3823
BstEII GGTNACC 1 cut(s) 1234
BstFNI CGCG 3 cut(s) 628, 876, 2645
BstH2I RGCGCY 1 cut(s) 4547
BstHHI GCGC 3 cut(s) 878, 2013, 4546
BstMCI CGRYCG 2 cut(s) 255, 501
BstNI CCWGG 9 cut(s) 155, 405, 718, 2831, 3486, 3885, 4122, 4199, 5091
BstPI GGTNACC 1 cut(s) 1234
BstSCI CCNGG 9 cut(s) 153, 403, 716, 2829, 3484, 3883, 4120, 4197, 5089
BstSFI CTRYAG 3 cut(s) 2799, 3177, 4770
BstSLI GKGCMC 1 cut(s) 459
BstSNI TACGTA 1 cut(s) 1589
BstUI CGCG 3 cut(s) 628, 876, 2645
BstV2I GAAGAC 8 cut(s) 660, 951, 1650, 2027, 2187, 2783, 4999, 5319
BstX2I RGATCY 6 cut(s) 150, 620, 1619, 2491, 3268, 4973
BstXI CCANNNNNNTGG 3 cut(s) 1298, 2534, 3322
BstYI RGATCY 6 cut(s) 150, 620, 1619, 2491, 3268, 4973
BstZ17I GTATAC 1 cut(s) 1607
Bsu36I CCTNAGG 1 cut(s) 1473
BsuI GTATCC 3 cut(s) 552, 2264, 3509
BtgI CCRYGG 6 cut(s) 321, 1007, 2515, 2643, 3735, 3823
BtrI CACGTC 2 cut(s) 160, 2207
BtsI GCAGTG 3 cut(s) 2091, 4146, 4428
BtsIMutI CAGTG 7 cut(s) 2091, 2814, 4072, 4146, 4428, 4830, 5211
BveI ACCTGC 2 cut(s) 3898, 4820
Cac8I GCNNGC 9 cut(s) 318, 358, 2274, 3160, 3343, 3801, 3873, 3928, 4380
CaiI CAGNNNCTG 3 cut(s) 521, 2030, 3431
CciI TCATGA 2 cut(s) 1372, 5061
CfoI GCGC 3 cut(s) 878, 2013, 4546
Cfr10I RCCGGY 1 cut(s) 4223
Cfr42I CCGCGG 1 cut(s) 2646
CseI GACGC 4 cut(s) 617, 1136, 3695, 5312
CspAI ACCGGT 1 cut(s) 4223
DraI TTTAAA 1 cut(s) 2355
DraIII CACNNNGTG 3 cut(s) 226, 1391, 5279
DrdI GACNNNNNNGTC 1 cut(s) 5399
DseDI GACNNNNNNGTC 1 cut(s) 5399
EaeI YGGCCR 3 cut(s) 406, 4195, 5092
EciI GGCGGA 1 cut(s) 981
Ecl136II GAGCTC 1 cut(s) 749
Eco105I TACGTA 1 cut(s) 1589
Eco130I CCWWGG 8 cut(s) 39, 1007, 2200, 2552, 3315, 3735, 3823, 5099
Eco24I GRGCYC 2 cut(s) 459, 751
Eco31I GGTCTC 3 cut(s) 1068, 3757, 4745
Eco32I GATATC 2 cut(s) 2863, 5180
Eco47I GGWCC 7 cut(s) 233, 1492, 1670, 2611, 3134, 3887, 4946
Eco47III AGCGCT 1 cut(s) 4545
Eco53kI GAGCTC 1 cut(s) 749
Eco57I CTGAAG 5 cut(s) 764, 1898, 2027, 3916, 4482
Eco72I CACGTG 1 cut(s) 184
Eco81I CCTNAGG 1 cut(s) 1473
Eco88I CYCGRG 1 cut(s) 557
Eco91I GGTNACC 1 cut(s) 1234
EcoICRI GAGCTC 1 cut(s) 749
EcoO109I RGGNCCY 1 cut(s) 233
EcoO65I GGTNACC 1 cut(s) 1234
EcoRI GAATTC 2 cut(s) 1302, 3562
EcoRII CCWGG 9 cut(s) 153, 403, 716, 2829, 3484, 3883, 4120, 4197, 5089
EcoRV GATATC 2 cut(s) 2863, 5180
EcoT14I CCWWGG 8 cut(s) 39, 1007, 2200, 2552, 3315, 3735, 3823, 5099
EcoT22I ATGCAT 2 cut(s) 2175, 3805
EcoT38I GRGCYC 2 cut(s) 459, 751
ErhI CCWWGG 8 cut(s) 39, 1007, 2200, 2552, 3315, 3735, 3823, 5099
Esp3I CGTCTC 4 cut(s) 978, 1120, 3018, 3907
FalI AAGNNNNNCTT 4 cut(s) 3240, 3272, 4972, 5004
FaqI GGGAC 6 cut(s) 878, 2509, 2570, 2597, 3988, 5152
FauI CCCGC 2 cut(s) 683, 2770
FbaI TGATCA 2 cut(s) 2887, 3139
FblI GTMKAC 4 cut(s) 255, 1269, 1606, 3634
FriOI GRGCYC 2 cut(s) 459, 751
GlaI GCGC 3 cut(s) 877, 2012, 4545
GsuI CTGGAG 4 cut(s) 2720, 3361, 3999, 4410
HaeII RGCGCY 1 cut(s) 4547
HapII CCGG 2 cut(s) 334, 4224
HgaI GACGC 4 cut(s) 617, 1136, 3695, 5312
HhaI GCGC 3 cut(s) 878, 2013, 4546
Hin1I GRCGYC 2 cut(s) 1113, 3687
Hin6I GCGC 3 cut(s) 876, 2011, 4544
HinP1I GCGC 3 cut(s) 876, 2011, 4544
HincII GTYRAC 6 cut(s) 256, 1897, 2089, 2974, 5256, 5392
HindII GTYRAC 6 cut(s) 256, 1897, 2089, 2974, 5256, 5392
HindIII AAGCTT 4 cut(s) 1740, 2228, 3198, 3928
HpaI GTTAAC 2 cut(s) 1897, 5256
HpaII CCGG 2 cut(s) 334, 4224
Hpy99I CGWCG 6 cut(s) 296, 356, 431, 452, 455, 703
Hsp92I GRCGYC 2 cut(s) 1113, 3687
HspAI GCGC 3 cut(s) 876, 2011, 4544
Ksp22I TGATCA 2 cut(s) 2887, 3139
KspAI GTTAAC 2 cut(s) 1897, 5256
KspI CCGCGG 1 cut(s) 2646
LguI GCTCTTC 2 cut(s) 306, 3900
LmnI GCTCC 9 cut(s) 10, 1702, 1709, 1857, 2129, 2780, 2875, 3325, 4120
MfeI CAATTG 6 cut(s) 900, 3809, 3864, 4128, 4529, 5235
MflI RGATCY 6 cut(s) 150, 620, 1619, 2491, 3268, 4973
MhlI GDGCHC 3 cut(s) 459, 751, 843
MlsI TGGCCA 2 cut(s) 4197, 5094
MluI ACGCGT 1 cut(s) 626
MluNI TGGCCA 2 cut(s) 4197, 5094
MlyI GAGTC 6 cut(s) 186, 521, 3320, 5067, 5203, 5258
MmeI TCCRAC 5 cut(s) 1195, 2515, 2664, 4085, 5320
Mox20I TGGCCA 2 cut(s) 4197, 5094
Mph1103I ATGCAT 2 cut(s) 2175, 3805
MroXI GAANNNNTTC 3 cut(s) 725, 830, 3714
MscI TGGCCA 2 cut(s) 4197, 5094
MslI CAYNNNNRTG 6 cut(s) 1232, 2422, 3393, 4383, 4418, 5016
Msp20I TGGCCA 2 cut(s) 4197, 5094
MspA1I CMGCKG 7 cut(s) 518, 1352, 1876, 2645, 2777, 3155, 4079
MspI CCGG 2 cut(s) 334, 4224
MspR9I CCNGG 9 cut(s) 155, 405, 718, 2831, 3486, 3885, 4122, 4199, 5091
MunI CAATTG 6 cut(s) 900, 3809, 3864, 4128, 4529, 5235
Mva1269I GAATGC 1 cut(s) 5407
MvaI CCWGG 9 cut(s) 155, 405, 718, 2831, 3486, 3885, 4122, 4199, 5091
MvnI CGCG 3 cut(s) 628, 876, 2645
NcoI CCATGG 3 cut(s) 1007, 3735, 3823
NheI GCTAGC 1 cut(s) 3158
NmeAIII GCCGAG 2 cut(s) 535, 3583
NmuCI GTSAC 7 cut(s) 1234, 2046, 3813, 4051, 5206, 5300, 5528
NsiI ATGCAT 2 cut(s) 2175, 3805
NspV TTCGAA 1 cut(s) 3657
OliI CACNNNNGTG 1 cut(s) 5016
PaeI GCATGC 2 cut(s) 3803, 4382
PagI TCATGA 2 cut(s) 1372, 5061
PciI ACATGT 4 cut(s) 191, 3002, 5002, 5322
PciSI GCTCTTC 2 cut(s) 306, 3900
PcsI WCGNNNNNNNCGW 2 cut(s) 507, 4990
PctI GAATGC 1 cut(s) 5407
PdmI GAANNNNTTC 3 cut(s) 725, 830, 3714
PflFI GACNNNGTC 1 cut(s) 350
PflMI CCANNNNNTGG 5 cut(s) 1698, 2147, 2263, 4715, 4811
PfoI TCCNGGA 4 cut(s) 153, 716, 3883, 4120
PinAI ACCGGT 1 cut(s) 4223
Ple19I CGATCG 1 cut(s) 501
PleI GAGTC 6 cut(s) 185, 520, 3319, 5066, 5203, 5257
PmaCI CACGTG 1 cut(s) 184
PmlI CACGTG 1 cut(s) 184
PpsI GAGTC 6 cut(s) 185, 520, 3319, 5066, 5203, 5257
Ppu21I YACGTR 3 cut(s) 184, 1589, 4994
PpuMI RGGWCCY 1 cut(s) 233
PscI ACATGT 4 cut(s) 191, 3002, 5002, 5322
PshBI ATTAAT 1 cut(s) 4026
PsiI TTATAA 2 cut(s) 4098, 5264
Psp124BI GAGCTC 1 cut(s) 751
Psp1406I AACGTT 1 cut(s) 4479
Psp5II RGGWCCY 1 cut(s) 233
Psp6I CCWGG 9 cut(s) 153, 403, 716, 2829, 3484, 3883, 4120, 4197, 5089
PspCI CACGTG 1 cut(s) 184
PspEI GGTNACC 1 cut(s) 1234
PspGI CCWGG 9 cut(s) 153, 403, 716, 2829, 3484, 3883, 4120, 4197, 5089
PspOMI GGGCCC 1 cut(s) 455
PspPPI RGGWCCY 1 cut(s) 233
PstI CTGCAG 1 cut(s) 4774
PstNI CAGNNNCTG 3 cut(s) 521, 2030, 3431
PsuI RGATCY 6 cut(s) 150, 620, 1619, 2491, 3268, 4973
PsyI GACNNNGTC 1 cut(s) 350
PvuI CGATCG 1 cut(s) 501
PvuII CAGCTG 4 cut(s) 518, 1352, 1876, 4079
RseI CAYNNNNRTG 6 cut(s) 1232, 2422, 3393, 4383, 4418, 5016
SacI GAGCTC 1 cut(s) 751
SacII CCGCGG 1 cut(s) 2646
SalI GTCGAC 1 cut(s) 254
SapI GCTCTTC 2 cut(s) 306, 3900
ScaI AGTACT 1 cut(s) 1638
SchI GAGTC 6 cut(s) 186, 521, 3320, 5067, 5203, 5258
ScrFI CCNGG 9 cut(s) 155, 405, 718, 2831, 3486, 3885, 4122, 4199, 5091
SduI GDGCHC 3 cut(s) 459, 751, 843
SfcI CTRYAG 3 cut(s) 2799, 3177, 4770
Sfr303I CCGCGG 1 cut(s) 2646
SfuI TTCGAA 1 cut(s) 3657
SgrBI CCGCGG 1 cut(s) 2646
SinI GGWCC 7 cut(s) 233, 1492, 1670, 2611, 3134, 3887, 4946
SmiMI CAYNNNNRTG 6 cut(s) 1232, 2422, 3393, 4383, 4418, 5016
SmlI CTYRAG 8 cut(s) 128, 638, 1534, 2540, 2963, 3299, 4301, 4539
SmoI CTYRAG 8 cut(s) 128, 638, 1534, 2540, 2963, 3299, 4301, 4539
SnaBI TACGTA 1 cut(s) 1589
SpeI ACTAGT 2 cut(s) 2740, 4237
SphI GCATGC 2 cut(s) 3803, 4382
SspI AATATT 4 cut(s) 2320, 3654, 4873, 5293
SstI GAGCTC 1 cut(s) 751
StyD4I CCNGG 9 cut(s) 153, 403, 716, 2829, 3484, 3883, 4120, 4197, 5089
StyI CCWWGG 8 cut(s) 39, 1007, 2200, 2552, 3315, 3735, 3823, 5099
TaqII GACCGA 1 cut(s) 241
TatI WGTACW 3 cut(s) 1636, 2239, 4393
TauI GCSGC 4 cut(s) 2348, 3290, 4778, 5078
TscAI CASTG 7 cut(s) 2098, 2821, 4072, 4146, 4435, 4830, 5211
TseFI GTSAC 7 cut(s) 1234, 2046, 3813, 4051, 5206, 5300, 5528
Tsp45I GTSAC 7 cut(s) 1234, 2046, 3813, 4051, 5206, 5300, 5528
TspGWI ACGGA 4 cut(s) 158, 621, 2504, 4929
TspRI CASTG 7 cut(s) 2098, 2821, 4072, 4146, 4435, 4830, 5211
Tth111I GACNNNGTC 1 cut(s) 350
Van91I CCANNNNNTGG 5 cut(s) 1698, 2147, 2263, 4715, 4811
VpaK11BI GGWCC 7 cut(s) 233, 1492, 1670, 2611, 3134, 3887, 4946
VspI ATTAAT 1 cut(s) 4026
XapI RAATTY 5 cut(s) 1302, 1918, 3531, 3562, 4627
XcmI CCANNNNNNNNNTGG 3 cut(s) 1502, 2680, 5511
XmaJI CCTAGG 2 cut(s) 2200, 2552
XmiI GTMKAC 4 cut(s) 255, 1269, 1606, 3634
XmnI GAANNNNTTC 3 cut(s) 725, 830, 3714
ZraI GACGTC 1 cut(s) 1114
ZrmI AGTACT 1 cut(s) 1638
Zsp2I ATGCAT 2 cut(s) 2175, 3805
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.