pycom16g22840

5'-nucleotidase domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0006733
Physical Location & Seq
Reverse (-)
23213217 .. 23216865
3649 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1074 bp
ATGGCCTGCGTTCGTAGGCTGCTTCCTCTCTGCTCTTCCATCAAGAGGACGAGCTTCCCATCTTCTGGTTCTGGAGCTCTGCAAGGTTTTCGAAGCTATGGAGCAATTGCTTCCGAGCAAACTCTTCCGAGCAATTTACCTGAAGCTGATACTTCAGTTCTTAATGATGAGATAGCACAGATTCGACGTGAATTTGATGCTGCGAAGCAGAGTTTCCTCAAGATTCCAGCCGCACTTAAAGCAATGCCTAAAATGAATCCGAAAGGGATATATGTAAATAAAAACCTGAGATTGGACAATATTCAAGTTTATGGATTCGACTATGATTACACACTGGCACATTATTCTGCCAATTTACAGAGCTTGATATATGATATGGCAAAGGAGCATATGGTAAATGAGTTTCGGTACCCTGAGGTTTGCATGGAATTTAAGTATGATTCGACTTTTCCCATTAGAGGATTGTATTATGATAAGCTAAAAGGGTGCCTATTGAAGCTGGATTTTTTTGGGTCAATTGAACCAGATGGATGCTACTATGGTCGTCGTAAGCTTAGCAGGAAGGAAATTGAAGAAATATATGGCACACGACATATTGGTCGTGATCAGGCCCAGGGACTTGTTGGGTTGATGGATTTCTTTTGCTTTAGCGAGGCATGCCTCATTTCAGATATCGTGCAATATTTTGTTGATGCTAAGCTGGAATTTGATGCTAGCTACATCTATCAAGATGTAAATCGTGCAATTCAGCATGTTCATCGCAGTGGCTTGGTTCATCGAGGAATTCTTTCTGACCCCCATAGATATCTAGTAAAAAATGGACAAGTGTTACACTTTCTCAAGATGCTCAGGGAGAAGGGAAAGAAACTATTCTTGCTGACTAACTCACCATATTACTTTGTGGACGGAGGAATGACCTTTATGTTGGAGGATTCTAAGGAATACAGAGACTCCTGGAGGGAACTTTTTGATGTTGTGATCGCTAAAGCCAATAAGCCTGATTTCTACACATCTGAGCATCCATTCCGGTTTGTATCTTCAACACTTTGTCTATCGCCATTTTATAAAGTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
5_nucleotid

Protein Analysis

358

Amino Acids

41.2

Weight (kDa)

8.45

Isoelectric Point (pI)

53.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
5_nucleotid PF05761 90 - 347 1.1e-95 5' nucleotidase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1065
AasI GACNNNNNNGTC 1 cut(s) 597
Acc65I GGTACC 1 cut(s) 408
AccB1I GGYRCC 2 cut(s) 408, 486
AccB7I CCANNNNNTGG 1 cut(s) 65
AciI CCGC 1 cut(s) 231
AcsI RAATTY 4 cut(s) 191, 428, 704, 783
AcuI CTGAAG 2 cut(s) 138, 162
AfaI GTAC 1 cut(s) 410
AfiI CCNNNNNNNGG 4 cut(s) 45, 65, 292, 458
AgsI TTSAA 5 cut(s) 305, 496, 521, 572, 1041
AjiI CACGTC 1 cut(s) 188
AjnI CCWGG 2 cut(s) 612, 953
Alw21I GWGCWC 1 cut(s) 79
Alw26I GTCTC 1 cut(s) 942
AoxI GGCC 2 cut(s) 3, 609
ApeKI GCWGC 2 cut(s) 19, 200
ApoI RAATTY 4 cut(s) 191, 428, 704, 783
Asp700I GAANNNNTTC 2 cut(s) 787, 869
Asp718I GGTACC 1 cut(s) 408
AspS9I GGNCC 1 cut(s) 610
AsuHPI GGTGA 1 cut(s) 879
AsuII TTCGAA 1 cut(s) 91
AsuNHI GCTAGC 1 cut(s) 713
AxyI CCTNAGG 1 cut(s) 414
BaeI ACNNNNGTAYC 2 cut(s) 141, 174
BanI GGYRCC 2 cut(s) 408, 486
BanII GRGCYC 1 cut(s) 79
Bbv12I GWGCWC 1 cut(s) 79
BbvI GCAGC 2 cut(s) 6, 187
BccI CCATC 4 cut(s) 47, 67, 521, 625
BcgI CGANNNNNNTGC 4 cut(s) 71, 105, 740, 774
BciT130I CCWGG 2 cut(s) 614, 955
BclI TGATCA 1 cut(s) 604
BcoDI GTCTC 1 cut(s) 942
BfaI CTAG 2 cut(s) 714, 809
BisI GCNGC 3 cut(s) 20, 201, 231
BlpI GCTNAGC 2 cut(s) 554, 696
BlsI GCNGC 3 cut(s) 21, 202, 232
Bme1390I CCNGG 2 cut(s) 614, 955
BmgBI CACGTC 1 cut(s) 188
BmgT120I GGNCC 1 cut(s) 610
BmiI GGNNCC 2 cut(s) 410, 488
BmrFI CCNGG 2 cut(s) 614, 955
BmsI GCATC 6 cut(s) 187, 521, 682, 700, 834, 1027
BmtI GCTAGC 1 cut(s) 717
BpmI CTGGAG 2 cut(s) 93, 976
Bpu10I CCTNAGC 1 cut(s) 848
Bpu1102I GCTNAGC 2 cut(s) 554, 696
Bpu14I TTCGAA 1 cut(s) 91
BpuEI CTTGAG 2 cut(s) 203, 824
BsaBI GATNNNNATC 1 cut(s) 735
BsaJI CCNNGG 2 cut(s) 612, 613
BsaWI WCCGGW 1 cut(s) 1026
BsaXI ACNNNNNCTCC 2 cut(s) 935, 965
Bsc4I CCNNNNNNNGG 4 cut(s) 45, 65, 292, 458
Bse1I ACTGG 1 cut(s) 339
Bse21I CCTNAGG 1 cut(s) 414
Bse3DI GCAATG 1 cut(s) 249
Bse8I GATNNNNATC 1 cut(s) 735
BseBI CCWGG 2 cut(s) 614, 955
BseDI CCNNGG 2 cut(s) 612, 613
BseGI GGATG 2 cut(s) 536, 1018
BseJI GATNNNNATC 1 cut(s) 735
BseLI CCNNNNNNNGG 4 cut(s) 45, 65, 292, 458
BseMI GCAATG 1 cut(s) 249
BseMII CTCAG 4 cut(s) 278, 405, 862, 1005
BseNI ACTGG 1 cut(s) 339
BseXI GCAGC 2 cut(s) 6, 187
BshFI GGCC 2 cut(s) 5, 611
BshNI GGYRCC 2 cut(s) 408, 486
BsiHKAI GWGCWC 1 cut(s) 79
BsiSI CCGG 1 cut(s) 1027
BslFI GGGAC 1 cut(s) 630
BslI CCNNNNNNNGG 4 cut(s) 45, 65, 292, 458
BsmAI GTCTC 1 cut(s) 942
BsmFI GGGAC 1 cut(s) 630
BsnI GGCC 2 cut(s) 5, 611
Bsp119I TTCGAA 1 cut(s) 91
Bsp1286I GDGCHC 1 cut(s) 79
Bsp143I GATC 2 cut(s) 604, 978
Bsp1720I GCTNAGC 2 cut(s) 554, 696
BspACI CCGC 1 cut(s) 231
BspANI GGCC 2 cut(s) 5, 611
BspCNI CTCAG 4 cut(s) 279, 406, 861, 1006
BspLI GGNNCC 2 cut(s) 410, 488
BspOI GCTAGC 1 cut(s) 717
BspQI GCTCTTC 1 cut(s) 40
BspT104I TTCGAA 1 cut(s) 91
BspT107I GGYRCC 2 cut(s) 408, 486
BsrDI GCAATG 1 cut(s) 249
BsrI ACTGG 1 cut(s) 339
BssECI CCNNGG 2 cut(s) 612, 613
BssMI GATC 2 cut(s) 604, 978
Bst2UI CCWGG 2 cut(s) 614, 955
Bst6I CTCTTC 2 cut(s) 40, 129
BstBI TTCGAA 1 cut(s) 91
BstC8I GCNNGC 3 cut(s) 7, 658, 715
BstDEI CTNAG 7 cut(s) 287, 414, 554, 696, 848, 936, 1014
BstF5I GGATG 2 cut(s) 536, 1018
BstKTI GATC 2 cut(s) 607, 981
BstMAI GTCTC 1 cut(s) 942
BstMBI GATC 2 cut(s) 604, 978
BstMWI GCNNNNNNNGC 2 cut(s) 239, 657
BstNI CCWGG 2 cut(s) 614, 955
BstNSI RCATGY 2 cut(s) 660, 755
BstSCI CCNGG 2 cut(s) 612, 953
BstV1I GCAGC 2 cut(s) 6, 187
Bsu36I CCTNAGG 1 cut(s) 414
BsuRI GGCC 2 cut(s) 5, 611
BtgZI GCGATG 1 cut(s) 743
BtrI CACGTC 1 cut(s) 188
BtsCI GGATG 2 cut(s) 536, 1018
BtsI GCAGTG 1 cut(s) 769
BtsIMutI CAGTG 2 cut(s) 332, 769
Cac8I GCNNGC 3 cut(s) 7, 658, 715
Cfr13I GGNCC 1 cut(s) 610
Csp6I GTAC 1 cut(s) 409
CviAII CATG 3 cut(s) 424, 657, 752
CviQI GTAC 1 cut(s) 409
DdeI CTNAG 7 cut(s) 287, 414, 554, 696, 848, 936, 1014
DpnI GATC 2 cut(s) 606, 980
DpnII GATC 2 cut(s) 604, 978
DrdI GACNNNNNNGTC 1 cut(s) 597
DseDI GACNNNNNNGTC 1 cut(s) 597
Eam1104I CTCTTC 2 cut(s) 40, 129
EarI CTCTTC 2 cut(s) 40, 129
Ecl136II GAGCTC 1 cut(s) 77
Eco24I GRGCYC 1 cut(s) 79
Eco32I GATATC 2 cut(s) 673, 806
Eco53kI GAGCTC 1 cut(s) 77
Eco57I CTGAAG 2 cut(s) 138, 162
Eco81I CCTNAGG 1 cut(s) 414
EcoICRI GAGCTC 1 cut(s) 77
EcoRI GAATTC 1 cut(s) 783
EcoRII CCWGG 2 cut(s) 612, 953
EcoRV GATATC 2 cut(s) 673, 806
EcoT38I GRGCYC 1 cut(s) 79
FaeI CATG 3 cut(s) 427, 660, 755
FaqI GGGAC 1 cut(s) 630
FatI CATG 3 cut(s) 423, 656, 751
FauNDI CATATG 1 cut(s) 390
FbaI TGATCA 1 cut(s) 604
Fnu4HI GCNGC 3 cut(s) 20, 201, 231
FokI GGATG 2 cut(s) 543, 1005
FriOI GRGCYC 1 cut(s) 79
Fsp4HI GCNGC 3 cut(s) 20, 201, 231
FspBI CTAG 2 cut(s) 714, 809
GluI GCNGC 3 cut(s) 20, 201, 231
GsuI CTGGAG 2 cut(s) 93, 976
HaeIII GGCC 2 cut(s) 5, 611
HapII CCGG 1 cut(s) 1027
Hin1II CATG 3 cut(s) 427, 660, 755
HindIII AAGCTT 1 cut(s) 551
HinfI GANTC 7 cut(s) 181, 223, 256, 315, 440, 932, 950
HpaII CCGG 1 cut(s) 1027
HphI GGTGA 1 cut(s) 879
Hpy166II GTNNAC 1 cut(s) 904
Hpy188I TCNGA 6 cut(s) 115, 129, 261, 670, 793, 1015
Hpy188III TCNNGA 6 cut(s) 43, 72, 220, 602, 728, 841
Hpy8I GTNNAC 1 cut(s) 904
Hpy99I CGWCG 2 cut(s) 189, 549
HpyAV CCTTC 2 cut(s) 556, 850
HpyCH4IV ACGT 1 cut(s) 187
HpyCH4V TGCA 4 cut(s) 82, 423, 679, 743
HpyF10VI GCNNNNNNNGC 2 cut(s) 239, 657
HpyF3I CTNAG 7 cut(s) 287, 414, 554, 696, 848, 936, 1014
HpySE526I ACGT 1 cut(s) 187
Hsp92II CATG 3 cut(s) 427, 660, 755
KpnI GGTACC 1 cut(s) 412
Ksp22I TGATCA 1 cut(s) 604
Kzo9I GATC 2 cut(s) 604, 978
LguI GCTCTTC 1 cut(s) 40
LmnI GCTCC 3 cut(s) 74, 101, 385
Lsp1109I GCAGC 2 cut(s) 6, 187
LweI GCATC 6 cut(s) 187, 521, 682, 700, 834, 1027
MaeI CTAG 2 cut(s) 714, 809
MaeII ACGT 1 cut(s) 187
MaeIII GTNAC 1 cut(s) 828
MalI GATC 2 cut(s) 606, 980
MboI GATC 2 cut(s) 604, 978
MboII GAAGA 5 cut(s) 27, 54, 116, 584, 1029
MfeI CAATTG 2 cut(s) 105, 516
MhlI GDGCHC 1 cut(s) 79
MlyI GAGTC 1 cut(s) 944
MmeI TCCRAC 1 cut(s) 906
MroXI GAANNNNTTC 2 cut(s) 787, 869
MseI TTAA 3 cut(s) 162, 237, 432
MslI CAYNNNNRTG 1 cut(s) 762
MspI CCGG 1 cut(s) 1027
MspR9I CCNGG 2 cut(s) 614, 955
MunI CAATTG 2 cut(s) 105, 516
MvaI CCWGG 2 cut(s) 614, 955
MwoI GCNNNNNNNGC 2 cut(s) 239, 657
NdeI CATATG 1 cut(s) 390
NdeII GATC 2 cut(s) 604, 978
NheI GCTAGC 1 cut(s) 713
NlaIII CATG 3 cut(s) 427, 660, 755
NlaIV GGNNCC 2 cut(s) 410, 488
NspI RCATGY 2 cut(s) 660, 755
NspV TTCGAA 1 cut(s) 91
PaeI GCATGC 1 cut(s) 660
PasI CCCWGGG 1 cut(s) 613
PciSI GCTCTTC 1 cut(s) 40
PdmI GAANNNNTTC 2 cut(s) 787, 869
PfeI GAWTC 6 cut(s) 181, 223, 256, 315, 440, 932
PflMI CCANNNNNTGG 1 cut(s) 65
PfoI TCCNGGA 1 cut(s) 953
PkrI GCNGC 3 cut(s) 21, 202, 232
PleI GAGTC 1 cut(s) 944
PpsI GAGTC 1 cut(s) 944
PsiI TTATAA 1 cut(s) 1065
Psp124BI GAGCTC 1 cut(s) 79
Psp6I CCWGG 2 cut(s) 612, 953
PspGI CCWGG 2 cut(s) 612, 953
PspN4I GGNNCC 2 cut(s) 410, 488
PspPI GGNCC 1 cut(s) 610
RsaI GTAC 1 cut(s) 410
RsaNI GTAC 1 cut(s) 409
RseI CAYNNNNRTG 1 cut(s) 762
SacI GAGCTC 1 cut(s) 79
SapI GCTCTTC 1 cut(s) 40
SaqAI TTAA 3 cut(s) 162, 237, 432
SatI GCNGC 3 cut(s) 20, 201, 231
Sau3AI GATC 2 cut(s) 604, 978
Sau96I GGNCC 1 cut(s) 610
SchI GAGTC 1 cut(s) 944
ScrFI CCNGG 2 cut(s) 614, 955
SduI GDGCHC 1 cut(s) 79
SfaNI GCATC 6 cut(s) 187, 521, 682, 700, 834, 1027
SfuI TTCGAA 1 cut(s) 91
SmiMI CAYNNNNRTG 1 cut(s) 762
SmlI CTYRAG 2 cut(s) 218, 839
SmoI CTYRAG 2 cut(s) 218, 839
SphI GCATGC 1 cut(s) 660
SsiI CCGC 1 cut(s) 231
SspI AATATT 2 cut(s) 301, 683
SspMI CTAG 2 cut(s) 714, 809
SstI GAGCTC 1 cut(s) 79
StyD4I CCNGG 2 cut(s) 612, 953
TaiI ACGT 1 cut(s) 190
TaqI TCGA 5 cut(s) 91, 184, 318, 443, 778
TauI GCSGC 1 cut(s) 233
TfiI GAWTC 6 cut(s) 181, 223, 256, 315, 440, 932
Tru1I TTAA 3 cut(s) 162, 237, 432
Tru9I TTAA 3 cut(s) 162, 237, 432
TscAI CASTG 2 cut(s) 339, 769
TseI GCWGC 2 cut(s) 19, 200
TspDTI ATGAA 3 cut(s) 269, 746, 764
TspGWI ACGGA 1 cut(s) 921
TspRI CASTG 2 cut(s) 339, 769
Van91I CCANNNNNTGG 1 cut(s) 65
XapI RAATTY 4 cut(s) 191, 428, 704, 783
XceI RCATGY 2 cut(s) 660, 755
XcmI CCANNNNNNNNNTGG 1 cut(s) 620
XmnI GAANNNNTTC 2 cut(s) 787, 869
XspI CTAG 2 cut(s) 714, 809
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.