pycom16g23290

chaperone protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0013028
Physical Location & Seq
Forward (+)
24015799 .. 24019482
3684 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 3186 bp
ATGAGAGCGGGGCTAAGTACGATCCAGCAAACTTTAACCCCAGAGGCGGCAAGCGTCTTGAACCACTCGATCGCCGAAGCAGGTCGACGGAACCACGGCCAGACGACGCCGCTACACGTGGCGGCAACCCTTTTGTCCTCCCCCACCGGCTTCCTCCGCCAAGCATGTATCAAATCTCACCCCAATTCCTCCCACCCTCTGCAGTGCCGTGCTCTGGAGCTCTGCTTCAGTGTGGCGCTGGAGCGGTTGCCCACCGCCCAAAACATGAGCCCGGGCATGGAGCCCCCCATCTCTAATGCTCTCATGGCTGCCTTGAAGCGAGCTCAGGCTCACCAGCGCCGGGGCTGCCCCGAACAGCAGCAGCAACCTCTCTTAGCAGTCAAAGTTGAGCTCGAGCAGCTCATCATCTCAATTCTCGATGACCCGAGTGTTAGTCGGGTCATGCGGGAGGCTAGTTTCTCTAGTCCTGCAGTCAAAGCCACCATTGAACAAACACTCAACTCTTCTGCTGCCTCCGCCGCCGTCAATTCTTCCCCAATTGGATTGCAGTTCCGCCCCGCTGGCCCCACTGTGCCACCCGCGAGCCGGAATTTGTATTTGAATCCGAGGCTGCAGCAGCCGCAGGGAGCAGCGGCACAATCGGGACAACGTAGAGGGGAGGAGGTCAAGAGGGTTGGTGATATTTTGTTGAGAACGAAGAAGAGGAACCCAGTTTTGGTTGGCGATTCGGAGCCTGAGGCGGTGACCAAAGAGCTCTTGAGGAGGATTCAGAGCAAGGAATTGGGCGAGGGGCCGCTGAAGAATGTTGAGGTCCTTCATTTGGAGAAGGAGGTTTCTTTGGATAGGAATCAGATAGTGTCAAAAATGAAGGAATTAGGGAGTTTGATTGAGACTCGGATGTTGAATTCGAATGGGGGAGGGGTCATTCTTGATTTGGGTGACTTGAAATGGCTTGTGGAGCAGAGTGCGGGCTTTGGAGTTGCAGCTCCGGGTCTAGGTTCGCCTCCTGTCCAGCAGCAGGTGGTTTCGGAGATAGGGCGGGCAGCAGTGGCGGAAATGGGGAAGCTGTTGGCGAGGTTTGGAGACGGTAGTGCTAATGGCAGCCGGCTTTGGTTAACTGGGACTGCTACTTGTGAGACATATTTGAGGTGCCAAGTCTATCACCCTTCAATGGAAACTGATTGGGATCTACAGGTAGTGCCAATTACTGGCAGAACGCCGCTTTCAGGATTGTTTCCGAGGATGGGGGCGACTAACGGGATCCTTAGTACCTCAGTTGGATCATTGTCACCAATGAAAGGTTTTCCACCGGCATCAATTGATCGACCAAGACTTATGTCAGAGAACTCGGATCCTGCTCGAAGAGCGCCATGTTGCCCTCAATGTACACATAGCTATGGGCAAGAACTTGCAAAACTGGTTGCCAAGGAATCTGAGACATCATCTTCTGAAACGGAGGCAGCTCAACCATTGCTACCACAGTGGTTGCAACATGCGAAAGCCCGTGATGTTCATTCCAGTGCATTAGATGAGACGCAGACTAAAGATCAAAATTTGATCCTGAAGCAGAAGACTCAAGAATTACAGAAGGAATGGGCAGATACATGTTTGCGTCTTCATCCTAATTTTCATCAGCCTAGTTTCAGCTCAGAGAGAATTATTCCAACCCTCTCAATGACGGGCTTGTACAATCCAAACTTGCTTGGTCGCCAACCATTCCAACCCAAATCACATCTGAATAAGAATCTTGGGACTCTGCAATTGAACACAAATCCGCCGACCAGCCAACCATCTGAACGGGCAATTTCCCAACCAGAAGGCCCTGTAAGGACAGAGCTGGTTCTTGGCCAAACAGAAGTCACTGAAACCACCCCTGAGCAAGCGCATAAAGAGCGCATCAGAGACTTTATGGGCTGCATGCCTTCTGAACCGCAAAACAAATTACATGGAATGCAAACGGAGGATAAACAATTGTGTCAAATAGATACTGAATCATTCAAAAAGCTCTGCAAAGGTTTAATGGAGGTGTGGTGGCAGCAAGAAGCAGCAACTGCTGTGGCTGAAACTGTGACAAAATGCAAGTTGGGTAATGGAAAGAGACATGGTGCCGGATCAAGGGGAGACATTTGGCTGCTGTTTATGGGCCCGGACAGTGTTGGCAAGAAGAAGATGGCATCAGCTCTTTCAGAACTGGTATCTGGGTCCAACCCAGTGATTATAAGTCTTAGCTCCCAACGTGGTAATTTGCAATCAGATATGAGTTTCCGCGGTAAAACAGTGGTGGATCGAATAGCAGAGACAGTTAAGAGAAACCCTTTTTCAGTAGTAGTGCTTGAGGACATTAATGAAGCTGACATGATGGTTCGTGGTAGCATAAAACGGGCAATTGAGAGAGGTCGTCTTGCTGATTCTTATGGCCGTGAAATCAGTCTTGGAAATGTTGTTTTCATCCTTACAGCAAATTGGTTGCCAGAAAATCTCAGACCCTTAACAAACAACAATTCACTTGAAGAAAAGCTTGCCAGTATAGCGAGGAGTGGTTGGCAGTTAAAGCTATCGGTTTGTGCAAGGGCTGCAAAACGGCGAGCCAATTGGTTAACGGATGAAGACAGGGCCACAAAGCCTAGGACTGATGCGGGTTCAGCATTAGGATTCGATCTGAATGAAGCTGCCAATGCTGAGGATGACAGGACAGATGGCTCGCTTAATTCAAGTGATCTGACCGTTGATCATGAAGATGACAATCGTCTCAATAACAGAGCCTTACTCAAAGTCACAACCCCGTCAGTACCCTGTGAATTGCTGGATACTGTTGATGATGCCATTGTGTTCAAGCCAATAGATTTCAACCCCATTCTGCAAAACATCACAAACACCATCAGAAAAAGATTCTCAAAGATAATGGGTGAGGGGATTTTATTTGAATTGCCAGAGGATGCTGTTGAAAAAATCTTGACTGGAATATGGCTAGGCCGGACTGGATTAGAGGAATGGGCAGAAAAGGTTCTTGCTCCGAGCATCCAGCAGCTTAAGTCCTGCCTTGGTGGCAGCACCGGTGTGATAGCCGATGAGTCAATGGTTGTTAGACTTGAATCCGATGGTGCTTCGGACTGCCTGAGCACCGGAGATAGGCTGCCCAGTAGCATCAATGTGGTTGTGGTGCCTGATGAGTTGAGACAACAATGA

Protein Analysis

1062

Amino Acids

115.81

Weight (kDa)

6.84

Isoelectric Point (pI)

48.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Clp_N PF02861 12 - 113 1.1e-06 Clp repeat (R) N-terminal domain
NBD_SMAX1 PF23569 219 - 404 1.2e-31 SMAX1 nucleotide binding domain
AAA_2 PF07724 711 - 824 8.7e-09 AAA domain (Cdc48 subfamily)
AAA_lid_SMAX1 PF26587 948 - 1034 1.8e-22 SMAX1-like, AAA+ ATPase lid domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013028)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G57710
fragaria_vesca FvH4_4g09820
malus_domestica MD13G1261900.v1.1 MD16G1264200.v1.1
prunus_persica Prupe.1G095200_v2.0.a1 Prupe.1G095200_v2.0.a1
pyrus_communis pycom13g24720 pycom16g23290
rosa_chinensis RchiOBHm_Chr4g0404601
rosa_laevigata RLG00000008920
rosa_multiflora Rmu_sc0002699.1_g000006
rosa_roxburghii Rroxscaffold_5G00348750
rosa_rugosa Rorug04G0047700
rosa_samantha Rh4AG123800 Rh4BG117200 Rh4CG131900 Rh4DG116700
rosa_wichuraiana Rw4G010030

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2219
AarI CACCTGC 1 cut(s) 1009
Acc36I ACCTGC 2 cut(s) 71, 1009
AccB1I GGYRCC 3 cut(s) 1149, 2105, 3160
AccB7I CCANNNNNTGG 1 cut(s) 1208
AccBSI CCGCTC 2 cut(s) 8, 244
AccI GTMKAC 1 cut(s) 85
AccII CGCG 2 cut(s) 581, 2268
AcoI YGGCCR 3 cut(s) 97, 1846, 2416
AcsI RAATTY 3 cut(s) 589, 904, 1552
AcuI CTGAAG 3 cut(s) 211, 818, 1583
AcvI CACGTG 1 cut(s) 118
AcyI GRCGYC 1 cut(s) 107
AfaI GTAC 5 cut(s) 19, 1270, 1387, 1688, 2790
AflII CTTAAG 1 cut(s) 3029
AflIII ACRYGT 2 cut(s) 115, 1604
AgeI ACCGGT 1 cut(s) 3053
AjuI GAANNNNNNNTTGG 4 cut(s) 698, 730, 2415, 2447
AleI CACNNNNGTG 1 cut(s) 3056
Alw21I GWGCWC 6 cut(s) 214, 222, 325, 393, 756, 3122
AlwNI CAGNNNCTG 1 cut(s) 2051
Ama87I CYCGRG 3 cut(s) 271, 392, 424
AoxI GGCC 9 cut(s) 97, 562, 791, 1819, 1846, 2143, 2416, 2613, 2971
ApaI GGGCCC 1 cut(s) 2147
ApoI RAATTY 3 cut(s) 589, 904, 1552
AseI ATTAAT 1 cut(s) 2343
AsiGI ACCGGT 1 cut(s) 3053
Asp700I GAANNNNTTC 1 cut(s) 3003
AspA2I CCTAGG 1 cut(s) 2624
AspLEI GCGC 5 cut(s) 238, 339, 1369, 1885, 1896
AspS9I GGNCC 8 cut(s) 563, 791, 811, 1820, 2143, 2144, 2202, 2613
AsuC2I CCSGG 5 cut(s) 272, 273, 341, 990, 2147
AsuHPI GGTGA 8 cut(s) 170, 323, 689, 754, 950, 1154, 1281, 2918
AsuII TTCGAA 1 cut(s) 908
AvaI CYCGRG 3 cut(s) 271, 392, 424
AvaII GGWCC 2 cut(s) 811, 2202
AvrII CCTAGG 1 cut(s) 2624
AxyI CCTNAGG 1 cut(s) 735
BaeGI GKGCMC 1 cut(s) 2147
BalI TGGCCA 1 cut(s) 1848
BamHI GGATCC 2 cut(s) 1260, 1351
BanI GGYRCC 3 cut(s) 1149, 2105, 3160
BanII GRGCYC 7 cut(s) 222, 272, 285, 325, 393, 756, 2147
BbrPI CACGTG 1 cut(s) 118
BbsI GAAGAC 3 cut(s) 1577, 1607, 2613
Bbv12I GWGCWC 6 cut(s) 214, 222, 325, 393, 756, 3122
BbvCI CCTCAGC 1 cut(s) 2679
BccI CCATC 8 cut(s) 296, 1237, 1798, 2164, 2353, 2690, 2885, 3092
BceAI ACGGC 5 cut(s) 112, 192, 506, 2403, 2597
BciVI GTATCC 1 cut(s) 2800
BclI TGATCA 1 cut(s) 2728
BcnI CCSGG 5 cut(s) 272, 273, 341, 990, 2147
BfaI CTAG 6 cut(s) 453, 462, 995, 1638, 2625, 2969
BfmI CTRYAG 4 cut(s) 200, 468, 611, 1190
BfoI RGCGCY 3 cut(s) 239, 340, 1370
BfrI CTTAAG 1 cut(s) 3029
BfuAI ACCTGC 2 cut(s) 71, 1009
BfuI GTATCC 1 cut(s) 2800
BglI GCCNNNNNGGC 2 cut(s) 561, 3045
BlnI CCTAGG 1 cut(s) 2624
Bme1390I CCNGG 5 cut(s) 272, 273, 341, 990, 2147
Bme18I GGWCC 2 cut(s) 811, 2202
BmeT110I CYCGRG 3 cut(s) 271, 392, 424
BmgT120I GGNCC 8 cut(s) 563, 791, 811, 1820, 2143, 2144, 2202, 2613
BmrFI CCNGG 5 cut(s) 272, 273, 341, 990, 2147
BmrI ACTGGG 4 cut(s) 704, 1128, 2204, 3132
BmsI GCATC 8 cut(s) 1322, 1905, 2183, 2623, 2809, 2926, 3027, 3153
BmuI ACTGGG 4 cut(s) 704, 1128, 2204, 3132
BoxI GACNNNNGTC 2 cut(s) 1336, 2745
BpiI GAAGAC 3 cut(s) 1577, 1607, 2613
BplI GAGNNNNNCTC 2 cut(s) 2751, 2783
BpmI CTGGAG 2 cut(s) 236, 260
Bpu10I CCTNAGC 4 cut(s) 324, 1875, 2679, 3116
Bpu14I TTCGAA 1 cut(s) 908
BpuEI CTTGAG 3 cut(s) 778, 1560, 2354
BpuMI CCSGG 5 cut(s) 272, 273, 341, 990, 2147
BsaAI YACGTR 1 cut(s) 118
BsaBI GATNNNNATC 3 cut(s) 846, 1185, 2742
BsaHI GRCGYC 1 cut(s) 107
BsaJI CCNNGG 9 cut(s) 94, 271, 340, 605, 1238, 1425, 2266, 2624, 3040
BsaWI WCCGGW 2 cut(s) 3053, 3122
BsaXI ACNNNNNCTCC 2 cut(s) 2015, 2045
Bse118I RCCGGY 4 cut(s) 146, 1104, 1309, 3053
Bse21I CCTNAGG 1 cut(s) 735
Bse3DI GCAATG 1 cut(s) 1469
Bse8I GATNNNNATC 3 cut(s) 846, 1185, 2742
BseDI CCNNGG 9 cut(s) 94, 271, 340, 605, 1238, 1425, 2266, 2624, 3040
BseGI GGATG 8 cut(s) 903, 1248, 1618, 2448, 2608, 2689, 2941, 3018
BseJI GATNNNNATC 3 cut(s) 846, 1185, 2742
BseMI GCAATG 1 cut(s) 1469
BseMII CTCAG 9 cut(s) 338, 726, 1287, 1425, 1662, 1866, 2494, 2670, 3107
BseRI GAGGAG 3 cut(s) 674, 775, 2548
BseSI GKGCMC 1 cut(s) 2147
Bsh1236I CGCG 2 cut(s) 581, 2268
Bsh1285I CGRYCG 1 cut(s) 72
BshFI GGCC 9 cut(s) 99, 564, 793, 1821, 1848, 2145, 2418, 2615, 2973
BshNI GGYRCC 3 cut(s) 1149, 2105, 3160
BshTI ACCGGT 1 cut(s) 3053
BsiEI CGRYCG 1 cut(s) 72
BsiHKAI GWGCWC 6 cut(s) 214, 222, 325, 393, 756, 3122
BsiHKCI CYCGRG 3 cut(s) 271, 392, 424
BslFI GGGAC 3 cut(s) 657, 1135, 1765
BsmBI CGTCTC 3 cut(s) 1077, 1526, 2753
BsmFI GGGAC 3 cut(s) 657, 1135, 1765
BsmI GAATGC 1 cut(s) 1956
BsnI GGCC 9 cut(s) 99, 564, 793, 1821, 1848, 2145, 2418, 2615, 2973
BsoBI CYCGRG 3 cut(s) 271, 392, 424
Bsp119I TTCGAA 1 cut(s) 908
Bsp120I GGGCCC 1 cut(s) 2143
Bsp1286I GDGCHC 9 cut(s) 214, 222, 272, 285, 325, 393, 756, 2147, 3122
Bsp1407I TGTACA 2 cut(s) 1385, 1686
BspANI GGCC 9 cut(s) 99, 564, 793, 1821, 1848, 2145, 2418, 2615, 2973
BspCNI CTCAG 9 cut(s) 337, 727, 1286, 1426, 1661, 1867, 2493, 2671, 3108
BspFNI CGCG 2 cut(s) 581, 2268
BspHI TCATGA 1 cut(s) 2731
BspMAI CTGCAG 3 cut(s) 204, 472, 615
BspMI ACCTGC 2 cut(s) 71, 1009
BspQI GCTCTTC 1 cut(s) 1357
BspT104I TTCGAA 1 cut(s) 908
BspT107I GGYRCC 3 cut(s) 1149, 2105, 3160
BspTI CTTAAG 1 cut(s) 3029
BsrBI CCGCTC 2 cut(s) 8, 244
BsrDI GCAATG 1 cut(s) 1469
BsrFI RCCGGY 4 cut(s) 146, 1104, 1309, 3053
BsrGI TGTACA 2 cut(s) 1385, 1686
BssAI RCCGGY 4 cut(s) 146, 1104, 1309, 3053
BssECI CCNNGG 9 cut(s) 94, 271, 340, 605, 1238, 1425, 2266, 2624, 3040
BssNI GRCGYC 1 cut(s) 107
BssT1I CCWWGG 3 cut(s) 1425, 2624, 3040
Bst4CI ACNGT 9 cut(s) 571, 1088, 1482, 2068, 2153, 2278, 2302, 2725, 2812
Bst6I CTCTTC 3 cut(s) 508, 695, 1357
BstACI GRCGYC 1 cut(s) 107
BstAFI CTTAAG 1 cut(s) 3029
BstAPI GCANNNNNTGC 2 cut(s) 2051, 2573
BstAUI TGTACA 2 cut(s) 1385, 1686
BstBAI YACGTR 1 cut(s) 118
BstBI TTCGAA 1 cut(s) 908
BstDSI CCRYGG 2 cut(s) 94, 2266
BstEII GGTNACC 1 cut(s) 742
BstF5I GGATG 8 cut(s) 903, 1248, 1618, 2448, 2608, 2689, 2941, 3018
BstFNI CGCG 2 cut(s) 581, 2268
BstH2I RGCGCY 3 cut(s) 239, 340, 1370
BstHHI GCGC 5 cut(s) 238, 339, 1369, 1885, 1896
BstMCI CGRYCG 1 cut(s) 72
BstNSI RCATGY 4 cut(s) 168, 1496, 1608, 1921
BstPAI GACNNNNGTC 2 cut(s) 1336, 2745
BstPI GGTNACC 1 cut(s) 742
BstSCI CCNGG 5 cut(s) 270, 271, 339, 988, 2145
BstSFI CTRYAG 4 cut(s) 200, 468, 611, 1190
BstSLI GKGCMC 1 cut(s) 2147
BstUI CGCG 2 cut(s) 581, 2268
BstV2I GAAGAC 3 cut(s) 1577, 1607, 2613
BstX2I RGATCY 3 cut(s) 1186, 1260, 1351
BstYI RGATCY 3 cut(s) 1186, 1260, 1351
Bsu36I CCTNAGG 1 cut(s) 735
BsuI GTATCC 1 cut(s) 2800
BsuRI GGCC 9 cut(s) 99, 564, 793, 1821, 1848, 2145, 2418, 2615, 2973
BtgI CCRYGG 2 cut(s) 94, 2266
BtsCI GGATG 8 cut(s) 903, 1248, 1618, 2448, 2608, 2689, 2941, 3018
BtsI GCAGTG 2 cut(s) 209, 1053
BveI ACCTGC 2 cut(s) 71, 1009
CaiI CAGNNNCTG 1 cut(s) 2051
CciI TCATGA 1 cut(s) 2731
CfoI GCGC 5 cut(s) 238, 339, 1369, 1885, 1896
Cfr10I RCCGGY 4 cut(s) 146, 1104, 1309, 3053
Cfr13I GGNCC 8 cut(s) 563, 791, 811, 1820, 2143, 2144, 2202, 2613
Cfr42I CCGCGG 1 cut(s) 2269
Cfr9I CCCGGG 1 cut(s) 271
CseI GACGC 4 cut(s) 43, 115, 1543, 1601
Csp6I GTAC 5 cut(s) 18, 1269, 1386, 1687, 2789
CspAI ACCGGT 1 cut(s) 3053
CspCI CAANNNNNGTGG 4 cut(s) 1467, 1502, 2037, 2072
CviQI GTAC 5 cut(s) 18, 1269, 1386, 1687, 2789
EaeI YGGCCR 3 cut(s) 97, 1846, 2416
Eam1104I CTCTTC 3 cut(s) 508, 695, 1357
EarI CTCTTC 3 cut(s) 508, 695, 1357
EciI GGCGGA 5 cut(s) 146, 505, 542, 1067, 1764
Ecl136II GAGCTC 4 cut(s) 220, 323, 391, 754
Eco130I CCWWGG 3 cut(s) 1425, 2624, 3040
Eco24I GRGCYC 7 cut(s) 222, 272, 285, 325, 393, 756, 2147
Eco47I GGWCC 2 cut(s) 811, 2202
Eco53kI GAGCTC 4 cut(s) 220, 323, 391, 754
Eco57I CTGAAG 3 cut(s) 211, 818, 1583
Eco72I CACGTG 1 cut(s) 118
Eco81I CCTNAGG 1 cut(s) 735
Eco88I CYCGRG 3 cut(s) 271, 392, 424
Eco91I GGTNACC 1 cut(s) 742
EcoICRI GAGCTC 4 cut(s) 220, 323, 391, 754
EcoO109I RGGNCCY 2 cut(s) 811, 1820
EcoO65I GGTNACC 1 cut(s) 742
EcoRI GAATTC 1 cut(s) 904
EcoT14I CCWWGG 3 cut(s) 1425, 2624, 3040
EcoT38I GRGCYC 7 cut(s) 222, 272, 285, 325, 393, 756, 2147
ErhI CCWWGG 3 cut(s) 1425, 2624, 3040
Esp3I CGTCTC 3 cut(s) 1077, 1526, 2753
FalI AAGNNNNNCTT 2 cut(s) 2502, 2534
FaqI GGGAC 3 cut(s) 657, 1135, 1765
FauI CCCGC 6 cut(s) 438, 565, 586, 961, 1032, 2629
FbaI TGATCA 1 cut(s) 2728
FblI GTMKAC 1 cut(s) 85
FokI GGATG 8 cut(s) 910, 1255, 1605, 2435, 2615, 2696, 2948, 3005
FriOI GRGCYC 7 cut(s) 222, 272, 285, 325, 393, 756, 2147
FspBI CTAG 6 cut(s) 453, 462, 995, 1638, 2625, 2969
GlaI GCGC 5 cut(s) 237, 338, 1368, 1884, 1895
GsuI CTGGAG 2 cut(s) 236, 260
HaeII RGCGCY 3 cut(s) 239, 340, 1370
HaeIII GGCC 9 cut(s) 99, 564, 793, 1821, 1848, 2145, 2418, 2615, 2973
HgaI GACGC 4 cut(s) 43, 115, 1543, 1601
HhaI GCGC 5 cut(s) 238, 339, 1369, 1885, 1896
Hin1I GRCGYC 1 cut(s) 107
Hin6I GCGC 5 cut(s) 236, 337, 1367, 1883, 1894
HinP1I GCGC 5 cut(s) 236, 337, 1367, 1883, 1894
HincII GTYRAC 3 cut(s) 86, 1116, 2598
HindII GTYRAC 3 cut(s) 86, 1116, 2598
HindIII AAGCTT 1 cut(s) 2516
HpaI GTTAAC 2 cut(s) 1116, 2598
HphI GGTGA 8 cut(s) 170, 323, 689, 754, 950, 1154, 1281, 2918
Hpy166II GTNNAC 4 cut(s) 86, 1116, 1388, 2598
Hpy8I GTNNAC 4 cut(s) 86, 1116, 1388, 2598
Hpy99I CGWCG 2 cut(s) 90, 109
HpyAV CCTTC 7 cut(s) 820, 824, 862, 1176, 1582, 1811, 1932
HpyCH4III ACNGT 9 cut(s) 571, 1088, 1482, 2068, 2153, 2278, 2302, 2725, 2812
HpyCH4IV ACGT 3 cut(s) 117, 649, 2236
HpySE526I ACGT 3 cut(s) 117, 649, 2236
Hsp92I GRCGYC 1 cut(s) 107
HspAI GCGC 5 cut(s) 236, 337, 1367, 1883, 1894
KroI GCCGGC 1 cut(s) 1104
KroNI GCCGGC 1 cut(s) 1106
Ksp22I TGATCA 1 cut(s) 2728
KspAI GTTAAC 2 cut(s) 1116, 2598
KspI CCGCGG 1 cut(s) 2269
LguI GCTCTTC 1 cut(s) 1357
LmnI GCTCC 9 cut(s) 217, 241, 280, 626, 730, 958, 991, 2234, 3016
LweI GCATC 8 cut(s) 1322, 1905, 2183, 2623, 2809, 2926, 3027, 3153
MaeI CTAG 6 cut(s) 453, 462, 995, 1638, 2625, 2969
MaeII ACGT 3 cut(s) 117, 649, 2236
MaeIII GTNAC 6 cut(s) 742, 938, 1287, 1858, 2068, 2773
MbiI CCGCTC 2 cut(s) 8, 244
MfeI CAATTG 6 cut(s) 537, 1317, 1760, 1970, 2385, 2590
MflI RGATCY 3 cut(s) 1186, 1260, 1351
MhlI GDGCHC 9 cut(s) 214, 222, 272, 285, 325, 393, 756, 2147, 3122
MlsI TGGCCA 1 cut(s) 1848
MluNI TGGCCA 1 cut(s) 1848
MlyI GAGTC 4 cut(s) 886, 1567, 1747, 3080
MmeI TCCRAC 4 cut(s) 1258, 1688, 1744, 2229
Mox20I TGGCCA 1 cut(s) 1848
MroNI GCCGGC 1 cut(s) 1104
MroXI GAANNNNTTC 1 cut(s) 3003
MscI TGGCCA 1 cut(s) 1848
MslI CAYNNNNRTG 5 cut(s) 1395, 1518, 2736, 3056, 3149
Msp20I TGGCCA 1 cut(s) 1848
MspA1I CMGCKG 4 cut(s) 560, 632, 796, 2268
MspCI CTTAAG 1 cut(s) 3029
MspR9I CCNGG 5 cut(s) 272, 273, 341, 990, 2147
MunI CAATTG 6 cut(s) 537, 1317, 1760, 1970, 2385, 2590
Mva1269I GAATGC 1 cut(s) 1956
MvnI CGCG 2 cut(s) 581, 2268
NaeI GCCGGC 1 cut(s) 1106
NciI CCSGG 5 cut(s) 272, 273, 341, 990, 2147
NgoMIV GCCGGC 1 cut(s) 1104
NmuCI GTSAC 6 cut(s) 742, 938, 1287, 1858, 2068, 2773
NspI RCATGY 4 cut(s) 168, 1496, 1608, 1921
NspV TTCGAA 1 cut(s) 908
OliI CACNNNNGTG 1 cut(s) 3056
PaeI GCATGC 1 cut(s) 1921
PaeR7I CTCGAG 1 cut(s) 392
PagI TCATGA 1 cut(s) 2731
PaqCI CACCTGC 1 cut(s) 1009
PciI ACATGT 1 cut(s) 1604
PciSI GCTCTTC 1 cut(s) 1357
PctI GAATGC 1 cut(s) 1956
PdiI GCCGGC 1 cut(s) 1106
PdmI GAANNNNTTC 1 cut(s) 3003
PflMI CCANNNNNTGG 1 cut(s) 1208
PinAI ACCGGT 1 cut(s) 3053
Ple19I CGATCG 1 cut(s) 72
PleI GAGTC 4 cut(s) 886, 1567, 1747, 3079
PmaCI CACGTG 1 cut(s) 118
PmlI CACGTG 1 cut(s) 118
PpsI GAGTC 4 cut(s) 886, 1567, 1747, 3079
Ppu21I YACGTR 1 cut(s) 118
PpuMI RGGWCCY 1 cut(s) 811
PscI ACATGT 1 cut(s) 1604
PshAI GACNNNNGTC 2 cut(s) 1336, 2745
PshBI ATTAAT 1 cut(s) 2343
PsiI TTATAA 1 cut(s) 2219
Psp124BI GAGCTC 4 cut(s) 222, 325, 393, 756
Psp5II RGGWCCY 1 cut(s) 811
PspCI CACGTG 1 cut(s) 118
PspEI GGTNACC 1 cut(s) 742
PspOMI GGGCCC 1 cut(s) 2143
PspPI GGNCC 8 cut(s) 563, 791, 811, 1820, 2143, 2144, 2202, 2613
PspPPI RGGWCCY 1 cut(s) 811
PspXI VCTCGAGB 1 cut(s) 392
PstI CTGCAG 3 cut(s) 204, 472, 615
PstNI CAGNNNCTG 1 cut(s) 2051
PsuI RGATCY 3 cut(s) 1186, 1260, 1351
PvuI CGATCG 1 cut(s) 72
RsaI GTAC 5 cut(s) 19, 1270, 1387, 1688, 2790
RsaNI GTAC 5 cut(s) 18, 1269, 1386, 1687, 2789
RseI CAYNNNNRTG 5 cut(s) 1395, 1518, 2736, 3056, 3149
SacI GAGCTC 4 cut(s) 222, 325, 393, 756
SacII CCGCGG 1 cut(s) 2269
SalI GTCGAC 1 cut(s) 84
SapI GCTCTTC 1 cut(s) 1357
Sau96I GGNCC 8 cut(s) 563, 791, 811, 1820, 2143, 2144, 2202, 2613
SchI GAGTC 4 cut(s) 886, 1567, 1747, 3080
ScrFI CCNGG 5 cut(s) 272, 273, 341, 990, 2147
SduI GDGCHC 9 cut(s) 214, 222, 272, 285, 325, 393, 756, 2147, 3122
SfaNI GCATC 8 cut(s) 1322, 1905, 2183, 2623, 2809, 2926, 3027, 3153
SfcI CTRYAG 4 cut(s) 200, 468, 611, 1190
Sfr274I CTCGAG 1 cut(s) 392
Sfr303I CCGCGG 1 cut(s) 2269
SfuI TTCGAA 1 cut(s) 908
SgrAI CRCCGGYG 1 cut(s) 3053
SgrBI CCGCGG 1 cut(s) 2269
SinI GGWCC 2 cut(s) 811, 2202
SlaI CTCGAG 1 cut(s) 392
SmaI CCCGGG 1 cut(s) 273
SmiMI CAYNNNNRTG 5 cut(s) 1395, 1518, 2736, 3056, 3149
SmlI CTYRAG 5 cut(s) 392, 757, 1575, 2333, 3029
SmoI CTYRAG 5 cut(s) 392, 757, 1575, 2333, 3029
SphI GCATGC 1 cut(s) 1921
SrfI GCCCGGGC 1 cut(s) 273
SspMI CTAG 6 cut(s) 453, 462, 995, 1638, 2625, 2969
SstI GAGCTC 4 cut(s) 222, 325, 393, 756
StyD4I CCNGG 5 cut(s) 270, 271, 339, 988, 2145
StyI CCWWGG 3 cut(s) 1425, 2624, 3040
TaaI ACNGT 9 cut(s) 571, 1088, 1482, 2068, 2153, 2278, 2302, 2725, 2812
TaiI ACGT 3 cut(s) 120, 652, 2239
TaqI TCGA 9 cut(s) 68, 85, 393, 417, 908, 1324, 1360, 2287, 2655
TatI WGTACW 2 cut(s) 1385, 1686
TauI GCSGC 8 cut(s) 50, 112, 125, 521, 622, 635, 796, 1222
TseFI GTSAC 6 cut(s) 742, 938, 1287, 1858, 2068, 2773
Tsp45I GTSAC 6 cut(s) 742, 938, 1287, 1858, 2068, 2773
TspGWI ACGGA 4 cut(s) 103, 1469, 1973, 2615
TspMI CCCGGG 1 cut(s) 271
Van91I CCANNNNNTGG 1 cut(s) 1208
Vha464I CTTAAG 1 cut(s) 3029
VpaK11BI GGWCC 2 cut(s) 811, 2202
VspI ATTAAT 1 cut(s) 2343
XapI RAATTY 3 cut(s) 589, 904, 1552
XceI RCATGY 4 cut(s) 168, 1496, 1608, 1921
XhoI CTCGAG 1 cut(s) 392
XmaI CCCGGG 1 cut(s) 271
XmaJI CCTAGG 1 cut(s) 2624
XmiI GTMKAC 1 cut(s) 85
XmnI GAANNNNTTC 1 cut(s) 3003
XspI CTAG 6 cut(s) 453, 462, 995, 1638, 2625, 2969
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.