pycom16g23340

5-formyltetrahydrofolate cyclo-ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0005536
Physical Location & Seq
Reverse (-)
24165488 .. 24167546
2059 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1023 bp
ATGGTACTATTTAATTTACCTTTTATTTTTTTTTCTTTAAATTATAGTCCTATCCGACCCGAACTCTTCAACCGCGCCTACGCGCAACCAAAGAGCGCCAAATTAACGGAAAAACCCGCAAAAGCACGAGAGCCACGAAGCATGTTCAGTCACAGCAGAGCAAGCGCGAGTGGGCTCATAAACGGGGCAAAGGAGGCGGTGGCGCTCATCACCCAGCCGCGCACGTTAGCTTCTCGTACCCTCTTCCCTTCTCGCTTAGCTAACGAACGCCTCGCATCCCATTCTCACCGCCCCTACGCCACCATGAACCGAGACGACAACGACCCAATCGAAGCTATATTCCGGCAGAAGCGGTTGCTACGGTCCAGAATCCGCAAAGAGCTCAAGAATATGGACCCGGTTCGGAGGTCCGAAGAAGATGCTGCAATTCAGAGCATTGTTTTAAATGCTCCCTGGTTCCAATCCAGTAAGAGCATATGCGCTTACATAAGCTCTTCTGCTTTACGAGAAGTCGATACGTCAAGAATTGTTTCAGAAATTCTATCCAAACCTGCCAATGAGGGTGATGTGCAGAACAGGAAAAAGCTTTATGTTCCGCGTGTGGAGGATAGGAATAGCAACATGAGGATGCTTAGGATTTCCAGCGTTGATGATCTGATCGTGAACTCGATGGACATTTTGGAGCCAGCTCTTTTGGATTCTGATGGGAAACAACACGAAGATGTTTTGGAGGCAAGCGATCCAGTTGACTTGTTCATCCTACCCGGGCTTGCATTTGACAGATGTGGAAGGCGTTTAGGCCGTGGTGGAGGTTACTATGATTTGTTCCTAAAGAAGTACCAAGAGCTTACAAAGCAACGACAATGGAAGGAGCCCCTTCGAGTTGCGCTGTCATATTCTGTACAGATCGTGGAGGAAGGAGCCATAGCTGTCACTTCAAATGATGTTTCTGTGGATGCTCTTGCATCTCCAGCCGGTGTCATTCCCATAAGCCCCGCTGCTCAGGAGAGGAGCATGGGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00670 ko01100 map00670 map01100
Pfam Domains
Protein Families
5-FTHF_cyc-lig

Protein Analysis

341

Amino Acids

38.16

Weight (kDa)

9.43

Isoelectric Point (pI)

54.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
5-FTHF_cyc-lig PF01812 117 - 322 2.2e-29 5-formyltetrahydrofolate cyclo-ligase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 559
AccII CGCG 5 cut(s) 75, 83, 167, 220, 598
AciI CCGC 9 cut(s) 73, 117, 197, 218, 289, 352, 373, 596, 996
AclWI GGATC 1 cut(s) 734
AcsI RAATTY 1 cut(s) 537
AfaI GTAC 4 cut(s) 6, 238, 839, 903
AgsI TTSAA 2 cut(s) 70, 939
AjnI CCWGG 1 cut(s) 452
AluBI AGCT 9 cut(s) 230, 260, 335, 382, 492, 586, 689, 847, 929
AluI AGCT 9 cut(s) 230, 260, 335, 382, 492, 586, 689, 847, 929
Alw21I GWGCWC 1 cut(s) 384
Alw26I GTCTC 1 cut(s) 306
AlwI GGATC 1 cut(s) 734
Ama87I CYCGRG 1 cut(s) 764
AoxI GGCC 1 cut(s) 799
ApeKI GCWGC 2 cut(s) 422, 998
ApoI RAATTY 1 cut(s) 537
Asp700I GAANNNNTTC 1 cut(s) 529
AspLEI GCGC 8 cut(s) 77, 85, 98, 167, 205, 222, 482, 889
AspS9I GGNCC 3 cut(s) 363, 394, 408
AsuC2I CCSGG 3 cut(s) 398, 765, 766
AsuHPI GGTGA 3 cut(s) 202, 278, 575
AvaI CYCGRG 1 cut(s) 764
AvaII GGWCC 3 cut(s) 363, 394, 408
BanII GRGCYC 3 cut(s) 177, 384, 876
BauI CACGAG 1 cut(s) 126
Bbv12I GWGCWC 1 cut(s) 384
BbvI GCAGC 2 cut(s) 409, 985
BccI CCATC 2 cut(s) 664, 698
BceAI ACGGC 1 cut(s) 786
BcgI CGANNNNNNTGC 2 cut(s) 401, 435
BciT130I CCWGG 1 cut(s) 454
BcnI CCSGG 3 cut(s) 398, 765, 766
BcoDI GTCTC 1 cut(s) 306
BfoI RGCGCY 2 cut(s) 99, 206
BfuAI ACCTGC 1 cut(s) 559
BisI GCNGC 3 cut(s) 218, 423, 999
BlpI GCTNAGC 1 cut(s) 256
BlsI GCNGC 3 cut(s) 219, 424, 1000
Bme1390I CCNGG 4 cut(s) 398, 454, 765, 766
Bme18I GGWCC 3 cut(s) 363, 394, 408
BmeT110I CYCGRG 1 cut(s) 764
BmgT120I GGNCC 3 cut(s) 363, 394, 408
BmiI GGNNCC 5 cut(s) 396, 458, 684, 873, 922
BmrFI CCNGG 4 cut(s) 398, 454, 765, 766
BmsI GCATC 5 cut(s) 284, 409, 618, 946, 974
BpmI CTGGAG 1 cut(s) 954
Bpu10I CCTNAGC 2 cut(s) 632, 1002
Bpu1102I GCTNAGC 1 cut(s) 256
BpuEI CTTGAG 1 cut(s) 368
BpuMI CCSGG 3 cut(s) 398, 765, 766
BsaJI CCNNGG 3 cut(s) 452, 764, 802
Bse118I RCCGGY 1 cut(s) 974
Bse1I ACTGG 2 cut(s) 465, 743
BseBI CCWGG 1 cut(s) 454
BseDI CCNNGG 3 cut(s) 452, 764, 802
BseGI GGATG 4 cut(s) 275, 633, 756, 961
BseMII CTCAG 1 cut(s) 1016
BseNI ACTGG 2 cut(s) 465, 743
BseXI GCAGC 2 cut(s) 409, 985
BseYI CCCAGC 1 cut(s) 213
BsgI GTGCAG 1 cut(s) 590
Bsh1236I CGCG 5 cut(s) 75, 83, 167, 220, 598
BshFI GGCC 1 cut(s) 801
BsiHKAI GWGCWC 1 cut(s) 384
BsiHKCI CYCGRG 1 cut(s) 764
BsiSI CCGG 4 cut(s) 343, 398, 765, 975
BsmAI GTCTC 1 cut(s) 306
BsmBI CGTCTC 1 cut(s) 306
BsnI GGCC 1 cut(s) 801
BsoBI CYCGRG 1 cut(s) 764
Bsp1286I GDGCHC 3 cut(s) 177, 384, 876
Bsp1407I TGTACA 1 cut(s) 901
Bsp143I GATC 4 cut(s) 652, 657, 739, 906
Bsp1720I GCTNAGC 1 cut(s) 256
BspACI CCGC 9 cut(s) 73, 117, 197, 218, 289, 352, 373, 596, 996
BspANI GGCC 1 cut(s) 801
BspCNI CTCAG 1 cut(s) 1015
BspFNI CGCG 5 cut(s) 75, 83, 167, 220, 598
BspLI GGNNCC 5 cut(s) 396, 458, 684, 873, 922
BspMI ACCTGC 1 cut(s) 559
BspPI GGATC 1 cut(s) 734
BspQI GCTCTTC 1 cut(s) 499
BsrFI RCCGGY 1 cut(s) 974
BsrGI TGTACA 1 cut(s) 901
BsrI ACTGG 2 cut(s) 465, 743
BssAI RCCGGY 1 cut(s) 974
BssECI CCNNGG 3 cut(s) 452, 764, 802
BssMI GATC 4 cut(s) 652, 657, 739, 906
BssSI CACGAG 1 cut(s) 126
Bst2BI CACGAG 1 cut(s) 126
Bst2UI CCWGG 1 cut(s) 454
Bst4CI ACNGT 1 cut(s) 363
Bst6I CTCTTC 3 cut(s) 71, 248, 499
BstAUI TGTACA 1 cut(s) 901
BstC8I GCNNGC 4 cut(s) 163, 687, 736, 771
BstDEI CTNAG 3 cut(s) 256, 632, 1002
BstDSI CCRYGG 1 cut(s) 802
BstF5I GGATG 4 cut(s) 275, 633, 756, 961
BstFNI CGCG 5 cut(s) 75, 83, 167, 220, 598
BstH2I RGCGCY 2 cut(s) 99, 206
BstHHI GCGC 8 cut(s) 77, 85, 98, 167, 205, 222, 482, 889
BstKTI GATC 4 cut(s) 655, 660, 742, 909
BstMAI GTCTC 1 cut(s) 306
BstMBI GATC 4 cut(s) 652, 657, 739, 906
BstMWI GCNNNNNNNGC 4 cut(s) 162, 194, 853, 971
BstNI CCWGG 1 cut(s) 454
BstNSI RCATGY 1 cut(s) 145
BstSCI CCNGG 4 cut(s) 396, 452, 763, 764
BstUI CGCG 5 cut(s) 75, 83, 167, 220, 598
BstV1I GCAGC 2 cut(s) 409, 985
BsuRI GGCC 1 cut(s) 801
BtgI CCRYGG 1 cut(s) 802
BtsCI GGATG 4 cut(s) 275, 633, 756, 961
BveI ACCTGC 1 cut(s) 559
Cac8I GCNNGC 4 cut(s) 163, 687, 736, 771
CfoI GCGC 8 cut(s) 77, 85, 98, 167, 205, 222, 482, 889
Cfr10I RCCGGY 1 cut(s) 974
Cfr13I GGNCC 3 cut(s) 363, 394, 408
Cfr9I CCCGGG 1 cut(s) 764
Csp6I GTAC 4 cut(s) 5, 237, 838, 902
CviAII CATG 4 cut(s) 142, 304, 622, 1015
CviQI GTAC 4 cut(s) 5, 237, 838, 902
DdeI CTNAG 3 cut(s) 256, 632, 1002
DpnI GATC 4 cut(s) 654, 659, 741, 908
DpnII GATC 4 cut(s) 652, 657, 739, 906
DraI TTTAAA 2 cut(s) 39, 444
Eam1104I CTCTTC 3 cut(s) 71, 248, 499
EarI CTCTTC 3 cut(s) 71, 248, 499
Ecl136II GAGCTC 1 cut(s) 382
Eco24I GRGCYC 3 cut(s) 177, 384, 876
Eco47I GGWCC 3 cut(s) 363, 394, 408
Eco53kI GAGCTC 1 cut(s) 382
Eco88I CYCGRG 1 cut(s) 764
EcoICRI GAGCTC 1 cut(s) 382
EcoRII CCWGG 1 cut(s) 452
EcoT38I GRGCYC 3 cut(s) 177, 384, 876
Esp3I CGTCTC 1 cut(s) 306
FaeI CATG 4 cut(s) 145, 307, 625, 1018
FatI CATG 4 cut(s) 141, 303, 621, 1014
FauI CCCGC 2 cut(s) 124, 1003
FauNDI CATATG 1 cut(s) 476
Fnu4HI GCNGC 3 cut(s) 218, 423, 999
FokI GGATG 4 cut(s) 262, 640, 743, 968
FriOI GRGCYC 3 cut(s) 177, 384, 876
Fsp4HI GCNGC 3 cut(s) 218, 423, 999
GlaI GCGC 8 cut(s) 76, 84, 97, 166, 204, 221, 481, 888
GluI GCNGC 3 cut(s) 218, 423, 999
GsaI CCCAGC 1 cut(s) 217
GsuI CTGGAG 1 cut(s) 954
HaeII RGCGCY 2 cut(s) 99, 206
HaeIII GGCC 1 cut(s) 801
HapII CCGG 4 cut(s) 343, 398, 765, 975
HhaI GCGC 8 cut(s) 77, 85, 98, 167, 205, 222, 482, 889
Hin1II CATG 4 cut(s) 145, 307, 625, 1018
Hin6I GCGC 8 cut(s) 75, 83, 96, 165, 203, 220, 480, 887
HinP1I GCGC 8 cut(s) 75, 83, 96, 165, 203, 220, 480, 887
HincII GTYRAC 1 cut(s) 748
HindII GTYRAC 1 cut(s) 748
HindIII AAGCTT 1 cut(s) 584
HinfI GANTC 2 cut(s) 369, 698
HpaII CCGG 4 cut(s) 343, 398, 765, 975
HphI GGTGA 3 cut(s) 202, 278, 575
Hpy166II GTNNAC 2 cut(s) 664, 748
Hpy188I TCNGA 7 cut(s) 56, 405, 412, 432, 535, 657, 703
Hpy188III TCNNGA 5 cut(s) 366, 385, 522, 661, 1004
Hpy8I GTNNAC 2 cut(s) 664, 748
HpyAV CCTTC 5 cut(s) 258, 783, 862, 887, 911
HpyCH4III ACNGT 1 cut(s) 363
HpyCH4IV ACGT 2 cut(s) 224, 518
HpyCH4V TGCA 4 cut(s) 425, 571, 773, 965
HpyF10VI GCNNNNNNNGC 4 cut(s) 162, 194, 853, 971
HpyF3I CTNAG 3 cut(s) 256, 632, 1002
HpySE526I ACGT 2 cut(s) 224, 518
Hsp92II CATG 4 cut(s) 145, 307, 625, 1018
HspAI GCGC 8 cut(s) 75, 83, 96, 165, 203, 220, 480, 887
Kzo9I GATC 4 cut(s) 652, 657, 739, 906
LguI GCTCTTC 1 cut(s) 499
LmnI GCTCC 5 cut(s) 454, 682, 871, 920, 1011
Lsp1109I GCAGC 2 cut(s) 409, 985
LweI GCATC 5 cut(s) 284, 409, 618, 946, 974
MaeII ACGT 2 cut(s) 224, 518
MaeIII GTNAC 3 cut(s) 149, 812, 931
MalI GATC 4 cut(s) 654, 659, 741, 908
MboI GATC 4 cut(s) 652, 657, 739, 906
MboII GAAGA 6 cut(s) 58, 235, 425, 428, 486, 731
MhlI GDGCHC 3 cut(s) 177, 384, 876
MluCI AATT 6 cut(s) 13, 40, 101, 426, 525, 537
MmeI TCCRAC 1 cut(s) 79
MroXI GAANNNNTTC 1 cut(s) 529
MseI TTAA 4 cut(s) 12, 38, 104, 443
MslI CAYNNNNRTG 2 cut(s) 626, 720
MspA1I CMGCKG 1 cut(s) 998
MspI CCGG 4 cut(s) 343, 398, 765, 975
MspR9I CCNGG 4 cut(s) 398, 454, 765, 766
MvaI CCWGG 1 cut(s) 454
MvnI CGCG 5 cut(s) 75, 83, 167, 220, 598
MwoI GCNNNNNNNGC 4 cut(s) 162, 194, 853, 971
NciI CCSGG 3 cut(s) 398, 765, 766
NdeI CATATG 1 cut(s) 476
NdeII GATC 4 cut(s) 652, 657, 739, 906
NlaIII CATG 4 cut(s) 145, 307, 625, 1018
NlaIV GGNNCC 5 cut(s) 396, 458, 684, 873, 922
NmuCI GTSAC 2 cut(s) 149, 931
NspI RCATGY 1 cut(s) 145
PciSI GCTCTTC 1 cut(s) 499
PcsI WCGNNNNNNNCGW 2 cut(s) 133, 327
PdmI GAANNNNTTC 1 cut(s) 529
PfeI GAWTC 2 cut(s) 369, 698
PkrI GCNGC 3 cut(s) 219, 424, 1000
Psp124BI GAGCTC 1 cut(s) 384
Psp6I CCWGG 1 cut(s) 452
PspFI CCCAGC 1 cut(s) 213
PspGI CCWGG 1 cut(s) 452
PspN4I GGNNCC 5 cut(s) 396, 458, 684, 873, 922
PspPI GGNCC 3 cut(s) 363, 394, 408
RsaI GTAC 4 cut(s) 6, 238, 839, 903
RsaNI GTAC 4 cut(s) 5, 237, 838, 902
RseI CAYNNNNRTG 2 cut(s) 626, 720
SacI GAGCTC 1 cut(s) 384
SapI GCTCTTC 1 cut(s) 499
SaqAI TTAA 4 cut(s) 12, 38, 104, 443
SatI GCNGC 3 cut(s) 218, 423, 999
Sau3AI GATC 4 cut(s) 652, 657, 739, 906
Sau96I GGNCC 3 cut(s) 363, 394, 408
ScrFI CCNGG 4 cut(s) 398, 454, 765, 766
SduI GDGCHC 3 cut(s) 177, 384, 876
SfaNI GCATC 5 cut(s) 284, 409, 618, 946, 974
SinI GGWCC 3 cut(s) 363, 394, 408
SmaI CCCGGG 1 cut(s) 766
SmiMI CAYNNNNRTG 2 cut(s) 626, 720
SmlI CTYRAG 1 cut(s) 383
SmoI CTYRAG 1 cut(s) 383
Sse9I AATT 6 cut(s) 13, 40, 101, 426, 525, 537
SsiI CCGC 9 cut(s) 73, 117, 197, 218, 289, 352, 373, 596, 996
SstI GAGCTC 1 cut(s) 384
StyD4I CCNGG 4 cut(s) 396, 452, 763, 764
TaaI ACNGT 1 cut(s) 363
TaiI ACGT 2 cut(s) 227, 521
TaqI TCGA 4 cut(s) 330, 513, 668, 880
TasI AATT 6 cut(s) 13, 40, 101, 426, 525, 537
TatI WGTACW 1 cut(s) 901
TauI GCSGC 1 cut(s) 220
TfiI GAWTC 2 cut(s) 369, 698
Tru1I TTAA 4 cut(s) 12, 38, 104, 443
Tru9I TTAA 4 cut(s) 12, 38, 104, 443
TseFI GTSAC 2 cut(s) 149, 931
TseI GCWGC 2 cut(s) 422, 998
Tsp45I GTSAC 2 cut(s) 149, 931
TspDTI ATGAA 2 cut(s) 320, 745
TspGWI ACGGA 1 cut(s) 122
TspMI CCCGGG 1 cut(s) 764
VpaK11BI GGWCC 3 cut(s) 363, 394, 408
XapI RAATTY 1 cut(s) 537
XceI RCATGY 1 cut(s) 145
XmaI CCCGGG 1 cut(s) 764
XmnI GAANNNNTTC 1 cut(s) 529
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.