pycom16g25600

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0012285
Physical Location & Seq
Reverse (-)
27601948 .. 27602820
873 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 873 bp
ATGGCGGATTTCTCCTTCTACCTCTCCGATACCGACGAATCAGCTGTGGACGACCTCATCTCCCAAGCCCAAGACCTCTGCGTTCTCGAGCAAGTTTCCGCCATCAACTGCTCCGGCTTCACCGACTCTGTCCTCCCCACTGAACTTGAATCCCGCTTCCGCAAACTCAAATCCTTCCCCGAGAGCTCCGCCTCCCGCCCGCCACCGTTAACCACCGGTTCAGCTCGGCGCAATTCTGTTGAAGGTAAGACAGACCCTGTTTCTGATGCTGCCATTTTCTCGCCTGCCAAACAGAACCGAGATGAGAAATTGGGGTCAAATCTCAAATCTGATCCCTCAGAATTTTCATTAGCGAAATCGAAATACAACCCAGGTGGGAAAAGGGGTTCAAAGGGGAAATTGAAAAATGGTTCAGTCTCATCTCCTTCAAATTCATCCAATTCCTCTCCGGAAAGTGCAATTTTCTCGCCATCCAAACAGAACCCAGATAAGAAGCAGCGTTCTAAGCCCAAACCGAAATCTCGGTCGTTTCATTCTCCGCCGGATTCTTCCAATTCGCTGACGGACTCCCCTTCGCCCCCGAGGAAATCGGGTTGCTTGTGGTGTTCCCCGAAGACAAAGAGGGCTTCTCACACAAAGAGTAAAGAAAAGGGGGCAATTGACTTCGCGGCTGGTCTGAATTTCAGCGACGATGATGGGCTTTTATCGGATTTGGGCAGTTTTTCGAAGAAAGAGCAGACGAGGAAGCTGAAGAAGGCGATGAAGGAGGAGGAGAAGATTTGCAGAGAGGCTGAGAAGATTGTCAAGTGCGCTAGGCAGGCGTCGGCAAGGATGAGTGCAGCTGCCATTGAAGATGAACTTAGTGATGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

291

Amino Acids

31.35

Weight (kDa)

9.08

Isoelectric Point (pI)

54.84

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012285)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G23270 AT1G31460
fragaria_vesca FvH4_5g24380
malus_domestica MD16G1288300.v1.1
prunus_persica Prupe.5G017000_v2.0.a1
pyrus_communis pycom16g25600
rosa_chinensis RchiOBHm_Chr7g0214771
rosa_laevigata RLG00000002723 RLG00000020760
rosa_multiflora Rmu_co8342721.1_g000001 Rmu_sc0002308.1_g000045 Rmu_sc0026107.1_g000001
rosa_roxburghii Rroxscaffold_3G00245100
rosa_rugosa Rorug06G0112700
rosa_samantha Rh7AG283900 Rh7BG274500 Rh7CG303300 Rh7DG287200
rosa_wichuraiana Rw7G024200

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 668
AccIII TCCGGA 1 cut(s) 448
AciI CCGC 9 cut(s) 5, 99, 154, 160, 189, 196, 200, 539, 668
AclWI GGATC 1 cut(s) 326
AcsI RAATTY 3 cut(s) 341, 430, 679
AcuI CTGAAG 1 cut(s) 770
AcyI GRCGYC 1 cut(s) 821
AgeI ACCGGT 1 cut(s) 215
AgsI TTSAA 6 cut(s) 149, 242, 390, 403, 429, 851
AjnI CCWGG 1 cut(s) 370
AloI GAACNNNNNNTCC 2 cut(s) 370, 402
AluBI AGCT 5 cut(s) 44, 186, 224, 748, 842
AluI AGCT 5 cut(s) 44, 186, 224, 748, 842
Alw21I GWGCWC 1 cut(s) 188
Alw26I GTCTC 1 cut(s) 421
AlwI GGATC 1 cut(s) 326
AlwNI CAGNNNCTG 1 cut(s) 257
Ama87I CYCGRG 3 cut(s) 86, 179, 580
Aor13HI TCCGGA 1 cut(s) 448
ApeKI GCWGC 4 cut(s) 269, 496, 839, 842
ApoI RAATTY 3 cut(s) 341, 430, 679
ArsI GACNNNNNNTTYG 2 cut(s) 509, 541
AsiGI ACCGGT 1 cut(s) 215
AspLEI GCGC 2 cut(s) 231, 812
AsuHPI GGTGA 1 cut(s) 112
AsuII TTCGAA 1 cut(s) 725
AvaI CYCGRG 3 cut(s) 86, 179, 580
BanII GRGCYC 1 cut(s) 188
BbsI GAAGAC 1 cut(s) 620
Bbv12I GWGCWC 1 cut(s) 188
BbvI GCAGC 4 cut(s) 256, 508, 829, 851
BccI CCATC 3 cut(s) 110, 478, 689
BcgI CGANNNNNNTGC 2 cut(s) 447, 481
BciT130I CCWGG 1 cut(s) 372
BcoDI GTCTC 1 cut(s) 421
BfaI CTAG 1 cut(s) 813
BisI GCNGC 5 cut(s) 270, 497, 669, 840, 843
BlsI GCNGC 5 cut(s) 271, 498, 670, 841, 844
Bme1390I CCNGG 1 cut(s) 372
BmeT110I CYCGRG 3 cut(s) 86, 179, 580
BmrFI CCNGG 1 cut(s) 372
BmsI GCATC 1 cut(s) 256
BpiI GAAGAC 1 cut(s) 620
BplI GAGNNNNNCTC 2 cut(s) 613, 645
Bpu14I TTCGAA 1 cut(s) 725
BsaHI GRCGYC 1 cut(s) 821
BsaJI CCNNGG 2 cut(s) 370, 581
BsaWI WCCGGW 2 cut(s) 215, 448
BsaXI ACNNNNNCTCC 2 cut(s) 44, 74
Bse118I RCCGGY 1 cut(s) 215
BseAI TCCGGA 1 cut(s) 448
BseBI CCWGG 1 cut(s) 372
BseDI CCNNGG 2 cut(s) 370, 581
BseGI GGATG 3 cut(s) 434, 470, 837
BseMII CTCAG 2 cut(s) 351, 783
BseRI GAGGAG 2 cut(s) 782, 785
BseXI GCAGC 4 cut(s) 256, 508, 829, 851
BsgI GTGCAG 1 cut(s) 858
Bsh1236I CGCG 1 cut(s) 668
Bsh1285I CGRYCG 1 cut(s) 527
BshTI ACCGGT 1 cut(s) 215
BsiEI CGRYCG 1 cut(s) 527
BsiHKAI GWGCWC 1 cut(s) 188
BsiHKCI CYCGRG 3 cut(s) 86, 179, 580
BsiSI CCGG 4 cut(s) 114, 216, 449, 542
BsmAI GTCTC 1 cut(s) 421
BsoBI CYCGRG 3 cut(s) 86, 179, 580
Bsp119I TTCGAA 1 cut(s) 725
Bsp1286I GDGCHC 1 cut(s) 188
Bsp13I TCCGGA 1 cut(s) 448
Bsp143I GATC 1 cut(s) 331
BspACI CCGC 9 cut(s) 5, 99, 154, 160, 189, 196, 200, 539, 668
BspCNI CTCAG 2 cut(s) 350, 784
BspEI TCCGGA 1 cut(s) 448
BspFNI CGCG 1 cut(s) 668
BspPI GGATC 1 cut(s) 326
BspT104I TTCGAA 1 cut(s) 725
BsrFI RCCGGY 1 cut(s) 215
BssAI RCCGGY 1 cut(s) 215
BssECI CCNNGG 2 cut(s) 370, 581
BssMI GATC 1 cut(s) 331
BssNI GRCGYC 1 cut(s) 821
Bst2UI CCWGG 1 cut(s) 372
Bst4CI ACNGT 1 cut(s) 207
BstACI GRCGYC 1 cut(s) 821
BstBI TTCGAA 1 cut(s) 725
BstC8I GCNNGC 3 cut(s) 200, 285, 819
BstDEI CTNAG 4 cut(s) 337, 504, 792, 860
BstF5I GGATG 3 cut(s) 434, 470, 837
BstFNI CGCG 1 cut(s) 668
BstHHI GCGC 2 cut(s) 231, 812
BstKTI GATC 1 cut(s) 334
BstMAI GTCTC 1 cut(s) 421
BstMBI GATC 1 cut(s) 331
BstMCI CGRYCG 1 cut(s) 527
BstMWI GCNNNNNNNGC 2 cut(s) 505, 818
BstNI CCWGG 1 cut(s) 372
BstSCI CCNGG 1 cut(s) 370
BstUI CGCG 1 cut(s) 668
BstV1I GCAGC 4 cut(s) 256, 508, 829, 851
BstV2I GAAGAC 1 cut(s) 620
BtgZI GCGATG 1 cut(s) 773
BtsCI GGATG 3 cut(s) 434, 470, 837
BtsIMutI CAGTG 1 cut(s) 138
Cac8I GCNNGC 3 cut(s) 200, 285, 819
CaiI CAGNNNCTG 1 cut(s) 257
CfoI GCGC 2 cut(s) 231, 812
Cfr10I RCCGGY 1 cut(s) 215
CseI GACGC 1 cut(s) 810
CspAI ACCGGT 1 cut(s) 215
CspCI CAANNNNNGTGG 4 cut(s) 127, 162, 355, 390
DdeI CTNAG 4 cut(s) 337, 504, 792, 860
DpnI GATC 1 cut(s) 333
DpnII GATC 1 cut(s) 331
EciI GGCGGA 4 cut(s) 20, 88, 178, 528
Ecl136II GAGCTC 1 cut(s) 186
Eco24I GRGCYC 1 cut(s) 188
Eco53kI GAGCTC 1 cut(s) 186
Eco57I CTGAAG 1 cut(s) 770
Eco88I CYCGRG 3 cut(s) 86, 179, 580
EcoICRI GAGCTC 1 cut(s) 186
EcoRII CCWGG 1 cut(s) 370
EcoT38I GRGCYC 1 cut(s) 188
FalI AAGNNNNNCTT 1 cut(s) 843
FauI CCCGC 3 cut(s) 161, 203, 207
Fnu4HI GCNGC 5 cut(s) 270, 497, 669, 840, 843
FokI GGATG 3 cut(s) 421, 457, 844
FriOI GRGCYC 1 cut(s) 188
Fsp4HI GCNGC 5 cut(s) 270, 497, 669, 840, 843
FspBI CTAG 1 cut(s) 813
GlaI GCGC 2 cut(s) 230, 811
GluI GCNGC 5 cut(s) 270, 497, 669, 840, 843
HapII CCGG 4 cut(s) 114, 216, 449, 542
HgaI GACGC 1 cut(s) 810
HhaI GCGC 2 cut(s) 231, 812
Hin1I GRCGYC 1 cut(s) 821
Hin6I GCGC 2 cut(s) 229, 810
HinP1I GCGC 2 cut(s) 229, 810
HincII GTYRAC 1 cut(s) 210
HindII GTYRAC 1 cut(s) 210
HinfI GANTC 5 cut(s) 38, 125, 149, 545, 566
HpaI GTTAAC 1 cut(s) 210
HpaII CCGG 4 cut(s) 114, 216, 449, 542
HphI GGTGA 1 cut(s) 112
Hpy166II GTNNAC 2 cut(s) 49, 210
Hpy188I TCNGA 6 cut(s) 28, 265, 331, 340, 678, 709
Hpy188III TCNNGA 2 cut(s) 86, 449
Hpy8I GTNNAC 2 cut(s) 49, 210
Hpy99I CGWCG 3 cut(s) 38, 692, 826
HpyAV CCTTC 7 cut(s) 25, 184, 236, 435, 582, 748, 757
HpyCH4III ACNGT 1 cut(s) 207
HpyCH4V TGCA 3 cut(s) 458, 783, 839
HpyF10VI GCNNNNNNNGC 2 cut(s) 505, 818
HpyF3I CTNAG 4 cut(s) 337, 504, 792, 860
Hsp92I GRCGYC 1 cut(s) 821
HspAI GCGC 2 cut(s) 229, 810
Kpn2I TCCGGA 1 cut(s) 448
KspAI GTTAAC 1 cut(s) 210
Kzo9I GATC 1 cut(s) 331
LmnI GCTCC 2 cut(s) 116, 191
Lsp1109I GCAGC 4 cut(s) 256, 508, 829, 851
LweI GCATC 1 cut(s) 256
MaeI CTAG 1 cut(s) 813
MalI GATC 1 cut(s) 333
MboI GATC 1 cut(s) 331
MboII GAAGA 7 cut(s) 540, 625, 739, 763, 787, 808, 863
MfeI CAATTG 1 cut(s) 657
MhlI GDGCHC 1 cut(s) 188
MlyI GAGTC 2 cut(s) 119, 560
MroI TCCGGA 1 cut(s) 448
MseI TTAA 1 cut(s) 209
MspA1I CMGCKG 2 cut(s) 44, 842
MspI CCGG 4 cut(s) 114, 216, 449, 542
MspR9I CCNGG 1 cut(s) 372
MunI CAATTG 1 cut(s) 657
MvaI CCWGG 1 cut(s) 372
MvnI CGCG 1 cut(s) 668
MwoI GCNNNNNNNGC 2 cut(s) 505, 818
NdeII GATC 1 cut(s) 331
NmeAIII GCCGAG 1 cut(s) 205
NspV TTCGAA 1 cut(s) 725
PaeR7I CTCGAG 1 cut(s) 86
PfeI GAWTC 3 cut(s) 38, 149, 545
PflFI GACNNNGTC 1 cut(s) 128
PinAI ACCGGT 1 cut(s) 215
PkrI GCNGC 5 cut(s) 271, 498, 670, 841, 844
PleI GAGTC 2 cut(s) 119, 560
PpsI GAGTC 2 cut(s) 119, 560
Psp124BI GAGCTC 1 cut(s) 188
Psp6I CCWGG 1 cut(s) 370
PspGI CCWGG 1 cut(s) 370
PstNI CAGNNNCTG 1 cut(s) 257
PsyI GACNNNGTC 1 cut(s) 128
PvuII CAGCTG 2 cut(s) 44, 842
SacI GAGCTC 1 cut(s) 188
SaqAI TTAA 1 cut(s) 209
SatI GCNGC 5 cut(s) 270, 497, 669, 840, 843
Sau3AI GATC 1 cut(s) 331
SchI GAGTC 2 cut(s) 119, 560
ScrFI CCNGG 1 cut(s) 372
SduI GDGCHC 1 cut(s) 188
SfaNI GCATC 1 cut(s) 256
Sfr274I CTCGAG 1 cut(s) 86
SfuI TTCGAA 1 cut(s) 725
SlaI CTCGAG 1 cut(s) 86
SmlI CTYRAG 1 cut(s) 86
SmoI CTYRAG 1 cut(s) 86
SsiI CCGC 9 cut(s) 5, 99, 154, 160, 189, 196, 200, 539, 668
SspMI CTAG 1 cut(s) 813
SstI GAGCTC 1 cut(s) 188
StyD4I CCNGG 1 cut(s) 370
TaaI ACNGT 1 cut(s) 207
TaqI TCGA 3 cut(s) 87, 359, 725
TaqII GACCGA 1 cut(s) 513
TauI GCSGC 1 cut(s) 671
TfiI GAWTC 3 cut(s) 38, 149, 545
Tru1I TTAA 1 cut(s) 209
Tru9I TTAA 1 cut(s) 209
TscAI CASTG 1 cut(s) 145
TseI GCWGC 4 cut(s) 269, 496, 839, 842
TspDTI ATGAA 5 cut(s) 336, 423, 521, 776, 870
TspGWI ACGGA 1 cut(s) 578
TspRI CASTG 1 cut(s) 145
Tth111I GACNNNGTC 1 cut(s) 128
XapI RAATTY 3 cut(s) 341, 430, 679
XhoI CTCGAG 1 cut(s) 86
XspI CTAG 1 cut(s) 813
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.