pycom17g00740

Enoyl-(Acyl carrier protein) reductase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0006083
Physical Location & Seq
Forward (+)
537100 .. 539512
2413 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 807 bp
ATGGGGAGTCCTCCTGCGAAGAAAGTCCTGCTCACCTCCAATGGTGACGAGATTTCACAGAGCATCGCCTTCTCTCTGGCCCAACGCGGCTGCAGGTTGGTGTTGATGGGGAAGGAGAGCTGCCTACGGGGTATTGTGCAGAAAATAACGGGCTCACTGGAGGGTGCGGTGGCGCCGGTGGAGGTGGTGGACGTGGACATGGAGGACGAGACGGAGGGAATTTTTGATGAGGCGGTGGAGAAGGCATGCAGCATCTTGGGCAATTTGGATGCATTTGTGCATTGCTATACTTACGAAGGAAAAATGCAAGACCAGCTACAATTAGCTGAAGGTGAATTCAAAAAGATAATGAAGATTAACTTTATGTCTGCATGGTTTCTGTTAAACGCTGTTGGCAGAAGAATGCGAGATTACAAGTCAGGAGGTTCAATCGTGTTCTTGACCTCGATAATTGGAGCCGAAAGGGGGCTTTATCCAGGAGCTGCTGCTTATAGTGCATGTTCGGCAGGGCTTCAGCAGTTAGCTAGGACGTCAGCCCTGGAGATTGGACGATACCAGATTAGGGTCAATGCTATTGCCCGTGGTTTGCATCTACAAGACGAATTTCCAATTTCTGTGGGAATGGAGAGAGCAAAGAAGCAGGTGATGGAGGCAGCTCCATTGGGGAGATGGCTTGATGTTAAAAACGATCTGGCTTCAACTGTGATATATCTAATCAGCGATGGGTCACGGTACATGACAGGCACAACAATATTTGTGGATGGGGCACAGTCTCTAACTAGGCCTCGGATGCGTTCTTTTATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
adh_short_C2

Protein Analysis

269

Amino Acids

29.31

Weight (kDa)

6.75

Isoelectric Point (pI)

36.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
adh_short PF00106 7 - 196 5.1e-24 short chain dehydrogenase
adh_short_C2 PF13561 18 - 255 1.8e-39 Enoyl-(Acyl carrier protein) reductase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 631
AatII GACGTC 1 cut(s) 533
Acc36I ACCTGC 2 cut(s) 84, 631
AccB1I GGYRCC 1 cut(s) 172
AccII CGCG 1 cut(s) 87
AciI CCGC 3 cut(s) 87, 167, 233
AcsI RAATTY 3 cut(s) 219, 335, 602
AcuI CTGAAG 2 cut(s) 348, 497
AcyI GRCGYC 2 cut(s) 173, 530
AfaI GTAC 1 cut(s) 734
AfiI CCNNNNNNNGG 2 cut(s) 465, 562
AgsI TTSAA 3 cut(s) 340, 429, 699
AjiI CACGTC 1 cut(s) 193
AjnI CCWGG 2 cut(s) 475, 537
AluBI AGCT 6 cut(s) 120, 316, 326, 482, 524, 656
AluI AGCT 6 cut(s) 120, 316, 326, 482, 524, 656
Alw26I GTCTC 2 cut(s) 203, 777
AlwNI CAGNNNCTG 1 cut(s) 482
AoxI GGCC 2 cut(s) 78, 782
ApeKI GCWGC 6 cut(s) 90, 120, 249, 482, 485, 653
ApoI RAATTY 3 cut(s) 219, 335, 602
AspLEI GCGC 1 cut(s) 175
AspS9I GGNCC 1 cut(s) 79
AsuHPI GGTGA 4 cut(s) 25, 56, 344, 655
BaeGI GKGCMC 1 cut(s) 769
BanI GGYRCC 1 cut(s) 172
BanII GRGCYC 1 cut(s) 155
BbvI GCAGC 6 cut(s) 77, 107, 261, 469, 472, 665
BccI CCATC 5 cut(s) 100, 640, 663, 716, 755
BciT130I CCWGG 2 cut(s) 477, 539
BcoDI GTCTC 2 cut(s) 203, 777
BfaI CTAG 2 cut(s) 525, 780
BfmI CTRYAG 1 cut(s) 91
BfoI RGCGCY 1 cut(s) 176
BfuAI ACCTGC 2 cut(s) 84, 631
BisI GCNGC 7 cut(s) 88, 91, 121, 250, 483, 486, 654
BlsI GCNGC 7 cut(s) 89, 92, 122, 251, 484, 487, 655
Bme1390I CCNGG 2 cut(s) 477, 539
BmgBI CACGTC 1 cut(s) 193
BmgT120I GGNCC 1 cut(s) 79
BmiI GGNNCC 2 cut(s) 174, 457
BmrFI CCNGG 2 cut(s) 477, 539
BmsI GCATC 5 cut(s) 72, 259, 261, 598, 780
BpmI CTGGAG 2 cut(s) 179, 560
BsaHI GRCGYC 2 cut(s) 173, 530
BsaJI CCNNGG 3 cut(s) 537, 580, 785
Bsc4I CCNNNNNNNGG 2 cut(s) 465, 562
Bse118I RCCGGY 1 cut(s) 175
Bse1I ACTGG 1 cut(s) 162
Bse3DI GCAATG 1 cut(s) 280
BseBI CCWGG 2 cut(s) 477, 539
BseDI CCNNGG 3 cut(s) 537, 580, 785
BseGI GGATG 3 cut(s) 274, 766, 795
BseLI CCNNNNNNNGG 2 cut(s) 465, 562
BseMI GCAATG 1 cut(s) 280
BseNI ACTGG 1 cut(s) 162
BseSI GKGCMC 1 cut(s) 769
BseXI GCAGC 6 cut(s) 77, 107, 261, 469, 472, 665
BsgI GTGCAG 1 cut(s) 158
Bsh1236I CGCG 1 cut(s) 87
BshFI GGCC 2 cut(s) 80, 784
BshNI GGYRCC 1 cut(s) 172
BsiSI CCGG 1 cut(s) 176
BslI CCNNNNNNNGG 2 cut(s) 465, 562
BsmAI GTCTC 2 cut(s) 203, 777
BsmBI CGTCTC 1 cut(s) 203
BsmI GAATGC 1 cut(s) 408
BsnI GGCC 2 cut(s) 80, 784
Bsp1286I GDGCHC 2 cut(s) 155, 769
Bsp143I GATC 1 cut(s) 688
BspACI CCGC 3 cut(s) 87, 167, 233
BspANI GGCC 2 cut(s) 80, 784
BspFNI CGCG 1 cut(s) 87
BspLI GGNNCC 2 cut(s) 174, 457
BspMAI CTGCAG 1 cut(s) 95
BspMI ACCTGC 2 cut(s) 84, 631
BspT107I GGYRCC 1 cut(s) 172
BsrDI GCAATG 1 cut(s) 280
BsrFI RCCGGY 1 cut(s) 175
BsrI ACTGG 1 cut(s) 162
BssAI RCCGGY 1 cut(s) 175
BssECI CCNNGG 3 cut(s) 537, 580, 785
BssMI GATC 1 cut(s) 688
BssNI GRCGYC 2 cut(s) 173, 530
Bst2UI CCWGG 2 cut(s) 477, 539
Bst4CI ACNGT 3 cut(s) 703, 732, 771
BstACI GRCGYC 2 cut(s) 173, 530
BstC8I GCNNGC 1 cut(s) 247
BstDSI CCRYGG 1 cut(s) 580
BstF5I GGATG 3 cut(s) 274, 766, 795
BstFNI CGCG 1 cut(s) 87
BstH2I RGCGCY 1 cut(s) 176
BstHHI GCGC 1 cut(s) 175
BstKTI GATC 1 cut(s) 691
BstMAI GTCTC 2 cut(s) 203, 777
BstMBI GATC 1 cut(s) 688
BstMWI GCNNNNNNNGC 5 cut(s) 258, 313, 494, 503, 790
BstNI CCWGG 2 cut(s) 477, 539
BstNSI RCATGY 2 cut(s) 249, 501
BstSCI CCNGG 2 cut(s) 475, 537
BstSFI CTRYAG 1 cut(s) 91
BstSLI GKGCMC 1 cut(s) 769
BstUI CGCG 1 cut(s) 87
BstV1I GCAGC 6 cut(s) 77, 107, 261, 469, 472, 665
BsuRI GGCC 2 cut(s) 80, 784
BtgI CCRYGG 1 cut(s) 580
BtgZI GCGATG 2 cut(s) 49, 735
BtrI CACGTC 1 cut(s) 193
BtsCI GGATG 3 cut(s) 274, 766, 795
BtsIMutI CAGTG 1 cut(s) 155
BveI ACCTGC 2 cut(s) 84, 631
Cac8I GCNNGC 1 cut(s) 247
CaiI CAGNNNCTG 1 cut(s) 482
CfoI GCGC 1 cut(s) 175
Cfr10I RCCGGY 1 cut(s) 175
Cfr13I GGNCC 1 cut(s) 79
Csp6I GTAC 1 cut(s) 733
CspCI CAANNNNNGTGG 4 cut(s) 597, 632, 738, 773
CviAII CATG 5 cut(s) 199, 246, 372, 498, 736
CviQI GTAC 1 cut(s) 733
DinI GGCGCC 1 cut(s) 174
DpnI GATC 1 cut(s) 690
DpnII GATC 1 cut(s) 688
Eco147I AGGCCT 1 cut(s) 784
Eco24I GRGCYC 1 cut(s) 155
Eco57I CTGAAG 2 cut(s) 348, 497
EcoRI GAATTC 1 cut(s) 335
EcoRII CCWGG 2 cut(s) 475, 537
EcoT22I ATGCAT 1 cut(s) 274
EcoT38I GRGCYC 1 cut(s) 155
EgeI GGCGCC 1 cut(s) 174
EheI GGCGCC 1 cut(s) 174
Esp3I CGTCTC 1 cut(s) 203
FaeI CATG 5 cut(s) 202, 249, 375, 501, 739
FalI AAGNNNNNCTT 2 cut(s) 344, 376
FatI CATG 5 cut(s) 198, 245, 371, 497, 735
Fnu4HI GCNGC 7 cut(s) 88, 91, 121, 250, 483, 486, 654
FokI GGATG 3 cut(s) 281, 773, 802
FriOI GRGCYC 1 cut(s) 155
Fsp4HI GCNGC 7 cut(s) 88, 91, 121, 250, 483, 486, 654
FspBI CTAG 2 cut(s) 525, 780
GlaI GCGC 1 cut(s) 174
GluI GCNGC 7 cut(s) 88, 91, 121, 250, 483, 486, 654
GsuI CTGGAG 2 cut(s) 179, 560
HaeII RGCGCY 1 cut(s) 176
HaeIII GGCC 2 cut(s) 80, 784
HapII CCGG 1 cut(s) 176
HhaI GCGC 1 cut(s) 175
Hin1I GRCGYC 2 cut(s) 173, 530
Hin1II CATG 5 cut(s) 202, 249, 375, 501, 739
Hin6I GCGC 1 cut(s) 173
HinP1I GCGC 1 cut(s) 173
HinfI GANTC 1 cut(s) 7
HpaII CCGG 1 cut(s) 176
HphI GGTGA 4 cut(s) 25, 56, 344, 655
Hpy166II GTNNAC 2 cut(s) 190, 196
Hpy188I TCNGA 1 cut(s) 789
Hpy188III TCNNGA 2 cut(s) 420, 439
Hpy8I GTNNAC 2 cut(s) 190, 196
HpyAV CCTTC 5 cut(s) 79, 106, 235, 290, 323
HpyCH4III ACNGT 3 cut(s) 703, 732, 771
HpyCH4IV ACGT 2 cut(s) 192, 530
HpyCH4V TGCA 9 cut(s) 93, 139, 249, 272, 280, 307, 371, 497, 589
HpyF10VI GCNNNNNNNGC 5 cut(s) 258, 313, 494, 503, 790
HpySE526I ACGT 2 cut(s) 192, 530
Hsp92I GRCGYC 2 cut(s) 173, 530
Hsp92II CATG 5 cut(s) 202, 249, 375, 501, 739
HspAI GCGC 1 cut(s) 173
KasI GGCGCC 1 cut(s) 172
Kzo9I GATC 1 cut(s) 688
LmnI GCTCC 3 cut(s) 455, 479, 661
Lsp1109I GCAGC 6 cut(s) 77, 107, 261, 469, 472, 665
LweI GCATC 5 cut(s) 72, 259, 261, 598, 780
MaeI CTAG 2 cut(s) 525, 780
MaeII ACGT 2 cut(s) 192, 530
MaeIII GTNAC 2 cut(s) 44, 726
MalI GATC 1 cut(s) 690
MboI GATC 1 cut(s) 688
MboII GAAGA 3 cut(s) 31, 364, 411
MhlI GDGCHC 2 cut(s) 155, 769
MluCI AATT 7 cut(s) 219, 262, 320, 335, 450, 602, 609
Mly113I GGCGCC 1 cut(s) 173
MlyI GAGTC 1 cut(s) 16
Mph1103I ATGCAT 1 cut(s) 274
MseI TTAA 3 cut(s) 357, 383, 681
MspI CCGG 1 cut(s) 176
MspR9I CCNGG 2 cut(s) 477, 539
Mva1269I GAATGC 1 cut(s) 408
MvaI CCWGG 2 cut(s) 477, 539
MvnI CGCG 1 cut(s) 87
MwoI GCNNNNNNNGC 5 cut(s) 258, 313, 494, 503, 790
NarI GGCGCC 1 cut(s) 173
NdeII GATC 1 cut(s) 688
NlaIII CATG 5 cut(s) 202, 249, 375, 501, 739
NlaIV GGNNCC 2 cut(s) 174, 457
NmuCI GTSAC 2 cut(s) 44, 726
NsiI ATGCAT 1 cut(s) 274
NspI RCATGY 2 cut(s) 249, 501
PaeI GCATGC 1 cut(s) 249
PaqCI CACCTGC 1 cut(s) 631
PceI AGGCCT 1 cut(s) 784
PctI GAATGC 1 cut(s) 408
PfoI TCCNGGA 1 cut(s) 475
PkrI GCNGC 7 cut(s) 89, 92, 122, 251, 484, 487, 655
PleI GAGTC 1 cut(s) 15
PluTI GGCGCC 1 cut(s) 176
PpsI GAGTC 1 cut(s) 15
Psp6I CCWGG 2 cut(s) 475, 537
PspGI CCWGG 2 cut(s) 475, 537
PspN4I GGNNCC 2 cut(s) 174, 457
PspPI GGNCC 1 cut(s) 79
PstI CTGCAG 1 cut(s) 95
PstNI CAGNNNCTG 1 cut(s) 482
RsaI GTAC 1 cut(s) 734
RsaNI GTAC 1 cut(s) 733
SaqAI TTAA 3 cut(s) 357, 383, 681
SatI GCNGC 7 cut(s) 88, 91, 121, 250, 483, 486, 654
Sau3AI GATC 1 cut(s) 688
Sau96I GGNCC 1 cut(s) 79
SchI GAGTC 1 cut(s) 16
ScrFI CCNGG 2 cut(s) 477, 539
SduI GDGCHC 2 cut(s) 155, 769
SfaNI GCATC 5 cut(s) 72, 259, 261, 598, 780
SfcI CTRYAG 1 cut(s) 91
SfoI GGCGCC 1 cut(s) 174
SgrAI CRCCGGYG 1 cut(s) 175
SphI GCATGC 1 cut(s) 249
Sse9I AATT 7 cut(s) 219, 262, 320, 335, 450, 602, 609
SseBI AGGCCT 1 cut(s) 784
SsiI CCGC 3 cut(s) 87, 167, 233
SspDI GGCGCC 1 cut(s) 172
SspI AATATT 1 cut(s) 753
SspMI CTAG 2 cut(s) 525, 780
StuI AGGCCT 1 cut(s) 784
StyD4I CCNGG 2 cut(s) 475, 537
TaaI ACNGT 3 cut(s) 703, 732, 771
TaiI ACGT 2 cut(s) 195, 533
TaqI TCGA 1 cut(s) 446
TasI AATT 7 cut(s) 219, 262, 320, 335, 450, 602, 609
TauI GCSGC 1 cut(s) 90
Tru1I TTAA 3 cut(s) 357, 383, 681
Tru9I TTAA 3 cut(s) 357, 383, 681
TscAI CASTG 1 cut(s) 162
TseFI GTSAC 2 cut(s) 44, 726
TseI GCWGC 6 cut(s) 90, 120, 249, 482, 485, 653
Tsp45I GTSAC 2 cut(s) 44, 726
TspDTI ATGAA 1 cut(s) 365
TspGWI ACGGA 1 cut(s) 227
TspRI CASTG 1 cut(s) 162
XapI RAATTY 3 cut(s) 219, 335, 602
XceI RCATGY 2 cut(s) 249, 501
XcmI CCANNNNNNNNNTGG 1 cut(s) 666
XspI CTAG 2 cut(s) 525, 780
ZraI GACGTC 1 cut(s) 531
Zsp2I ATGCAT 1 cut(s) 274
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.