pycom17g01640

Cleavage site for pathogenic type III effector avirulence factor Avr

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0005964
Physical Location & Seq
Forward (+)
1065772 .. 1066540
769 bp
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UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 405 bp
ATGCTAACTACTAACTACTCACGTCACAATTTATGCCATCCATTTGCATTGGTTCAAGCTTTAGCTACGATCTCCTTTTCTTCCTCCCTTATCACATACAACTCACGCCTCCTCTCTCTCGACATTCTTCTTCTCTTCCAGTCTCGTTTTCCTCTGAATCTCCGCGTTTGCACGCCGCTCCTCCTGGGAATGGCCTCGCAGGAAACGGGACGGCCCTTGCCTAAATTTGGAGAGTGGGATGTGAACAACCCAGCATCAGCTGAAGGGTTTACTATGATATTTACCAGAGCTAGAGATCAGAAGATGACCAATGGGCCGGCTGACAATGGGACTCCGGCTCCCTCTTCAACCAAATCAATAAACAATCAATATCCCCAAGAGAAAAAATGGTTTTGCTGTTTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AvrRpt-cleavage

Protein Analysis

135

Amino Acids

14.99

Weight (kDa)

9.07

Isoelectric Point (pI)

47.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AvrRpt-cleavage PF05627 70 - 102 1.4e-17 Cleavage site for pathogenic type III effector avirulence factor Avr
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 178
AccII CGCG 1 cut(s) 165
AciI CCGC 2 cut(s) 163, 176
AcsI RAATTY 1 cut(s) 224
AcuI CTGAAG 1 cut(s) 282
AfiI CCNNNNNNNGG 2 cut(s) 190, 227
AgsI TTSAA 2 cut(s) 56, 348
AjiI CACGTC 1 cut(s) 23
AjnI CCWGG 1 cut(s) 183
AluBI AGCT 4 cut(s) 59, 65, 260, 290
AluI AGCT 4 cut(s) 59, 65, 260, 290
Alw26I GTCTC 1 cut(s) 147
AoxI GGCC 3 cut(s) 192, 212, 314
ApoI RAATTY 1 cut(s) 224
AspS9I GGNCC 2 cut(s) 213, 314
BccI CCATC 1 cut(s) 45
BceAI ACGGC 1 cut(s) 227
BciT130I CCWGG 1 cut(s) 185
BcoDI GTCTC 1 cut(s) 147
BfaI CTAG 1 cut(s) 291
BisI GCNGC 1 cut(s) 176
BlsI GCNGC 1 cut(s) 177
Bme1390I CCNGG 1 cut(s) 185
BmgBI CACGTC 1 cut(s) 23
BmgT120I GGNCC 2 cut(s) 213, 314
BmiI GGNNCC 1 cut(s) 339
BmrFI CCNGG 1 cut(s) 185
BmsI GCATC 1 cut(s) 263
BsaJI CCNNGG 1 cut(s) 184
BsaXI ACNNNNNCTCC 2 cut(s) 322, 352
Bsc4I CCNNNNNNNGG 2 cut(s) 190, 227
Bse118I RCCGGY 1 cut(s) 316
Bse1I ACTGG 1 cut(s) 139
BseBI CCWGG 1 cut(s) 185
BseDI CCNNGG 1 cut(s) 184
BseGI GGATG 2 cut(s) 37, 244
BseLI CCNNNNNNNGG 2 cut(s) 190, 227
BseNI ACTGG 1 cut(s) 139
BseRI GAGGAG 2 cut(s) 101, 170
BseYI CCCAGC 1 cut(s) 250
Bsh1236I CGCG 1 cut(s) 165
BshFI GGCC 3 cut(s) 194, 214, 316
BsiSI CCGG 2 cut(s) 317, 335
BslFI GGGAC 2 cut(s) 222, 343
BslI CCNNNNNNNGG 2 cut(s) 190, 227
BsmAI GTCTC 1 cut(s) 147
BsmFI GGGAC 2 cut(s) 222, 343
BsnI GGCC 3 cut(s) 194, 214, 316
Bsp143I GATC 2 cut(s) 69, 295
BspACI CCGC 2 cut(s) 163, 176
BspANI GGCC 3 cut(s) 194, 214, 316
BspFNI CGCG 1 cut(s) 165
BspLI GGNNCC 1 cut(s) 339
BsrBI CCGCTC 1 cut(s) 178
BsrFI RCCGGY 1 cut(s) 316
BsrI ACTGG 1 cut(s) 139
BssAI RCCGGY 1 cut(s) 316
BssECI CCNNGG 1 cut(s) 184
BssMI GATC 2 cut(s) 69, 295
Bst2UI CCWGG 1 cut(s) 185
Bst6I CTCTTC 2 cut(s) 140, 349
BstC8I GCNNGC 2 cut(s) 173, 318
BstF5I GGATG 2 cut(s) 37, 244
BstFNI CGCG 1 cut(s) 165
BstKTI GATC 2 cut(s) 72, 298
BstMAI GTCTC 1 cut(s) 147
BstMBI GATC 2 cut(s) 69, 295
BstNI CCWGG 1 cut(s) 185
BstSCI CCNGG 1 cut(s) 183
BstUI CGCG 1 cut(s) 165
BsuRI GGCC 3 cut(s) 194, 214, 316
BtrI CACGTC 1 cut(s) 23
BtsCI GGATG 2 cut(s) 37, 244
Cac8I GCNNGC 2 cut(s) 173, 318
Cfr10I RCCGGY 1 cut(s) 316
Cfr13I GGNCC 2 cut(s) 213, 314
CviJI RGCY 9 cut(s) 59, 65, 194, 214, 260, 290, 316, 320, 338
CviKI_1 RGCY 9 cut(s) 59, 65, 194, 214, 260, 290, 316, 320, 338
DpnI GATC 2 cut(s) 71, 297
DpnII GATC 2 cut(s) 69, 295
Eam1104I CTCTTC 2 cut(s) 140, 349
EarI CTCTTC 2 cut(s) 140, 349
Eco57I CTGAAG 1 cut(s) 282
EcoRII CCWGG 1 cut(s) 183
FaiI YATR 3 cut(s) 34, 97, 275
FaqI GGGAC 2 cut(s) 222, 343
Fnu4HI GCNGC 1 cut(s) 176
FokI GGATG 2 cut(s) 24, 251
Fsp4HI GCNGC 1 cut(s) 176
FspBI CTAG 1 cut(s) 291
GluI GCNGC 1 cut(s) 176
GsaI CCCAGC 1 cut(s) 254
HaeIII GGCC 3 cut(s) 194, 214, 316
HapII CCGG 2 cut(s) 317, 335
HindIII AAGCTT 1 cut(s) 57
HinfI GANTC 2 cut(s) 157, 331
HpaII CCGG 2 cut(s) 317, 335
Hpy166II GTNNAC 2 cut(s) 244, 270
Hpy188I TCNGA 2 cut(s) 156, 300
Hpy188III TCNNGA 1 cut(s) 119
Hpy8I GTNNAC 2 cut(s) 244, 270
HpyAV CCTTC 1 cut(s) 257
HpyCH4IV ACGT 1 cut(s) 22
HpyCH4V TGCA 2 cut(s) 47, 171
HpySE526I ACGT 1 cut(s) 22
KroI GCCGGC 1 cut(s) 316
KroNI GCCGGC 1 cut(s) 318
Kzo9I GATC 2 cut(s) 69, 295
LmnI GCTCC 2 cut(s) 183, 343
LpnPI CCDG 8 cut(s) 152, 170, 185, 197, 264, 298, 330, 348
LweI GCATC 1 cut(s) 263
MaeI CTAG 1 cut(s) 291
MaeII ACGT 1 cut(s) 22
MaeIII GTNAC 1 cut(s) 23
MalI GATC 2 cut(s) 71, 297
MbiI CCGCTC 1 cut(s) 178
MboI GATC 2 cut(s) 69, 295
MboII GAAGA 6 cut(s) 72, 119, 122, 127, 313, 336
MluCI AATT 2 cut(s) 28, 224
MlyI GAGTC 1 cut(s) 325
MnlI CCTC 7 cut(s) 94, 119, 122, 162, 191, 205, 352
MroNI GCCGGC 1 cut(s) 316
MspA1I CMGCKG 1 cut(s) 260
MspI CCGG 2 cut(s) 317, 335
MspR9I CCNGG 1 cut(s) 185
MvaI CCWGG 1 cut(s) 185
MvnI CGCG 1 cut(s) 165
NaeI GCCGGC 1 cut(s) 318
NdeII GATC 2 cut(s) 69, 295
NgoMIV GCCGGC 1 cut(s) 316
NlaIV GGNNCC 1 cut(s) 339
NmuCI GTSAC 1 cut(s) 23
PdiI GCCGGC 1 cut(s) 318
PfeI GAWTC 1 cut(s) 157
PkrI GCNGC 1 cut(s) 177
PleI GAGTC 1 cut(s) 325
PpsI GAGTC 1 cut(s) 325
Psp6I CCWGG 1 cut(s) 183
PspFI CCCAGC 1 cut(s) 250
PspGI CCWGG 1 cut(s) 183
PspN4I GGNNCC 1 cut(s) 339
PspPI GGNCC 2 cut(s) 213, 314
PvuII CAGCTG 1 cut(s) 260
SatI GCNGC 1 cut(s) 176
Sau3AI GATC 2 cut(s) 69, 295
Sau96I GGNCC 2 cut(s) 213, 314
SchI GAGTC 1 cut(s) 325
ScrFI CCNGG 1 cut(s) 185
SetI ASST 5 cut(s) 25, 61, 67, 262, 292
SfaNI GCATC 1 cut(s) 263
Sse9I AATT 2 cut(s) 28, 224
SsiI CCGC 2 cut(s) 163, 176
SspMI CTAG 1 cut(s) 291
StyD4I CCNGG 1 cut(s) 183
TaiI ACGT 1 cut(s) 25
TaqI TCGA 1 cut(s) 120
TasI AATT 2 cut(s) 28, 224
TauI GCSGC 1 cut(s) 178
TfiI GAWTC 1 cut(s) 157
TseFI GTSAC 1 cut(s) 23
Tsp45I GTSAC 1 cut(s) 23
XapI RAATTY 1 cut(s) 224
XspI CTAG 1 cut(s) 291
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.