pycom17g01730

glycerol-3-phosphate dehydrogenase NAD(

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0014375
Physical Location & Seq
Forward (+)
1127767 .. 1130254
2488 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1233 bp
ATGGCATCTCCGCCCCGACGGGTGTCTTCCCTCTTATATTCTCTGTCTCCTCTCCTTCTCTCTCGCTCCTCGTATACCTATTCCTCTATCATCCACGCGTTGCCCTTTTGCCCTCCTCTCTTTCTCTCTCCTTTCCTCTCTCTCTCCTCTTCATCCATGGCTCCAGAACTCCAACAGGAGGAAGATTACACTACTGGCCCTGAAGCTCCCCACAAAACCAGAGTCACCGTCGTCGGCAGTGGCAACTGGGGTAGTGTTGCCGCCAAACTCATTGCCTCCAACACCCTCAGGCTCTCTTCCTTCCACGATGAAGTGAGAATGTGGGTGTTTGAGGAAACATTACCAAGTGGTGAGAAGCTCACAGATGCCATCAACCGCAACAATGAAAATGTGAAATACCTGCCCGGCATTAAGCTGGGGAAAAATGTTGTTGCAGACCCAGACCTTGAGACTGCAGTGAACGGTGCGAACATGCTGGTGTTTGTCACTCCACATCAATTTATGGAGGGTATATGCGGGAGGCTTGTTGGGAAGGTAAAAGCGGATGTGGAGGCAATTTCCCTCATCAAAGGAATGGAGGTCAAGATGGAAGGCCCGTGCATGATCTCGACACTCATCTCACAGCAATTGGGTATTAATTGTTGTGTTCTGATGGGAGCAAACATAGCTAATGAGATTGCTGTGGAGAAATTTAGTGAAGCCACAGTTGGATACAGAGAGAACAAGGAGATTGCACAGAAATGGGTTCAGCTCTTTAGTACTTCCTACTTCATAGTCACACCTATCCAAGATGTGGAAGGGGTAGAACTATGTGGAACCCTGAAAAATGTTGTGGCCATAGCAGCAGGTTTTGTGGATGGGTTGGAGATGGGAAATAACACAAAGGCTGCAATTATGAGAATCGGTCTAAGAGAGATGAAGGCATTTTCCAAGATGTTATTTTCATCTGTCAAGGACACTACCTTCTTCGAAAGCTGTGGTGTCGCTGATCTCATCACAACATGCCTGGGAGGAAGAAACAGGAAAGTTGCGGAAGCTTTTGCAAGGAGTGGAGGGAAAAGATCGTTTGATGAGCTTGAAGCAGAAATGCTGCAGGGTCAAAAATTACAGGGTGTCTCAACCGCAAAAGAGGTTTACGAGGTTTTAAGCCACCGCGGGTGGCTAGATTTCTTCCCACTTTTTGCAACAGTTCATGATCGATCTGCATTGGCACTCTTCCTCCATCAGCCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00564 ko01110 ko04011 map00564 map01110 map04011
Pfam Domains
Protein Families
NAD_Gly3P_dh_C NAD_Gly3P_dh_N

Protein Analysis

411

Amino Acids

44.85

Weight (kDa)

6.05

Isoelectric Point (pI)

36.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAD_Gly3P_dh_N PF01210 75 - 242 1.8e-40 NAD-dependent glycerol-3-phosphate dehydrogenase N-terminus
NAD_Gly3P_dh_C PF07479 264 - 398 5e-42 NAD-dependent glycerol-3-phosphate dehydrogenase C-terminus
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014375)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G40610
fragaria_vesca FvH4_6g52110 FvH4_6g52110
malus_domestica MD09G1020100.v1.1 MD17G1020500.v1.1 MD17G1020700.v1.1
prunus_persica Prupe.3G296200_v2.0.a1 Prupe.3G296200_v2.0.a1
pyrus_communis pycom111g01680 pycom17g01730
rosa_chinensis RchiOBHm_Chr2g0173621
rosa_laevigata RLG00000022206
rosa_multiflora Rmu_sc0002538.1_g000016
rosa_roxburghii Rroxscaffold_2G00078740
rosa_rugosa Rorug02G0571700
rosa_samantha Rh2DG676300
rosa_wichuraiana Rw2G053400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 408, 836
AccB7I CCANNNNNTGG 1 cut(s) 793
AccI GTMKAC 1 cut(s) 74
AccII CGCG 2 cut(s) 98, 1155
AciI CCGC 9 cut(s) 11, 261, 376, 516, 542, 1031, 1122, 1153, 1155
AcoI YGGCCR 1 cut(s) 834
AcsI RAATTY 1 cut(s) 689
AcuI CTGAAG 1 cut(s) 222
AfaI GTAC 1 cut(s) 760
AfiI CCNNNNNNNGG 2 cut(s) 178, 793
AflIII ACRYGT 1 cut(s) 96
AgsI TTSAA 1 cut(s) 1079
AjnI CCWGG 1 cut(s) 1005
AjuI GAANNNNNNNTTGG 2 cut(s) 690, 722
AluBI AGCT 8 cut(s) 206, 358, 415, 668, 751, 975, 1037, 1075
AluI AGCT 8 cut(s) 206, 358, 415, 668, 751, 975, 1037, 1075
Alw26I GTCTC 3 cut(s) 51, 443, 1120
AoxI GGCC 3 cut(s) 196, 592, 834
ApeKI GCWGC 3 cut(s) 842, 887, 1090
ApoI RAATTY 1 cut(s) 689
ArsI GACNNNNNNTTYG 2 cut(s) 207, 239
AseI ATTAAT 1 cut(s) 636
AspS9I GGNCC 2 cut(s) 197, 593
AsuC2I CCSGG 1 cut(s) 405
AsuHPI GGTGA 2 cut(s) 217, 362
AsuII TTCGAA 1 cut(s) 969
AxyI CCTNAGG 1 cut(s) 287
BalI TGGCCA 1 cut(s) 836
BbsI GAAGAC 1 cut(s) 18
BbvI GCAGC 3 cut(s) 854, 874, 1077
BccI CCATC 6 cut(s) 377, 580, 646, 851, 862, 1230
BciT130I CCWGG 1 cut(s) 1007
BciVI GTATCC 1 cut(s) 704
BcnI CCSGG 1 cut(s) 405
BcoDI GTCTC 3 cut(s) 51, 443, 1120
BfaI CTAG 1 cut(s) 1163
BfmI CTRYAG 2 cut(s) 453, 1091
BfuAI ACCTGC 2 cut(s) 408, 836
BfuI GTATCC 1 cut(s) 704
BisI GCNGC 4 cut(s) 261, 843, 888, 1091
BlsI GCNGC 4 cut(s) 262, 844, 889, 1092
BmcAI AGTACT 1 cut(s) 760
Bme1390I CCNGG 2 cut(s) 405, 1007
BmgT120I GGNCC 2 cut(s) 197, 593
BmiI GGNNCC 2 cut(s) 162, 817
BmrFI CCNGG 2 cut(s) 405, 1007
BmrI ACTGGG 1 cut(s) 256
BmsI GCATC 2 cut(s) 14, 355
BmuI ACTGGG 1 cut(s) 256
BoxI GACNNNNGTC 1 cut(s) 22
BpiI GAAGAC 1 cut(s) 18
BpmI CTGGAG 1 cut(s) 147
Bpu14I TTCGAA 1 cut(s) 969
BpuEI CTTGAG 1 cut(s) 467
BpuMI CCSGG 1 cut(s) 405
Bsa29I ATCGAT 1 cut(s) 1198
BsaJI CCNNGG 3 cut(s) 156, 1006, 1153
BsaXI ACNNNNNCTCC 2 cut(s) 1203, 1233
Bsc4I CCNNNNNNNGG 2 cut(s) 178, 793
Bse1I ACTGG 2 cut(s) 199, 251
Bse21I CCTNAGG 1 cut(s) 287
Bse3DI GCAATG 1 cut(s) 270
BseBI CCWGG 1 cut(s) 1007
BseCI ATCGAT 1 cut(s) 1198
BseDI CCNNGG 3 cut(s) 156, 1006, 1153
BseGI GGATG 4 cut(s) 90, 152, 550, 862
BseLI CCNNNNNNNGG 2 cut(s) 178, 793
BseMI GCAATG 1 cut(s) 270
BseMII CTCAG 1 cut(s) 301
BseNI ACTGG 2 cut(s) 199, 251
BseRI GAGGAG 4 cut(s) 39, 58, 105, 136
BseXI GCAGC 3 cut(s) 854, 874, 1077
BseYI CCCAGC 1 cut(s) 415
Bsh1236I CGCG 2 cut(s) 98, 1155
BshFI GGCC 3 cut(s) 198, 594, 836
BshVI ATCGAT 1 cut(s) 1198
BsiSI CCGG 1 cut(s) 405
BslI CCNNNNNNNGG 2 cut(s) 178, 793
BsmAI GTCTC 3 cut(s) 51, 443, 1120
BsnI GGCC 3 cut(s) 198, 594, 836
Bsp119I TTCGAA 1 cut(s) 969
Bsp143I GATC 5 cut(s) 603, 988, 1061, 1195, 1199
Bsp19I CCATGG 1 cut(s) 156
BspACI CCGC 9 cut(s) 11, 261, 376, 516, 542, 1031, 1122, 1153, 1155
BspANI GGCC 3 cut(s) 198, 594, 836
BspCNI CTCAG 1 cut(s) 300
BspDI ATCGAT 1 cut(s) 1198
BspFNI CGCG 2 cut(s) 98, 1155
BspHI TCATGA 1 cut(s) 1192
BspLI GGNNCC 2 cut(s) 162, 817
BspMAI CTGCAG 2 cut(s) 457, 1095
BspMI ACCTGC 2 cut(s) 408, 836
BspT104I TTCGAA 1 cut(s) 969
BsrDI GCAATG 1 cut(s) 270
BsrI ACTGG 2 cut(s) 199, 251
BssECI CCNNGG 3 cut(s) 156, 1006, 1153
BssMI GATC 5 cut(s) 603, 988, 1061, 1195, 1199
BssNAI GTATAC 1 cut(s) 75
BssT1I CCWWGG 1 cut(s) 156
Bst1107I GTATAC 1 cut(s) 75
Bst2UI CCWGG 1 cut(s) 1007
Bst4CI ACNGT 4 cut(s) 229, 464, 706, 1189
Bst6I CTCTTC 3 cut(s) 154, 301, 1220
BstBI TTCGAA 1 cut(s) 969
BstDEI CTNAG 2 cut(s) 287, 908
BstDSI CCRYGG 2 cut(s) 156, 1153
BstF5I GGATG 4 cut(s) 90, 152, 550, 862
BstFNI CGCG 2 cut(s) 98, 1155
BstKTI GATC 5 cut(s) 606, 991, 1064, 1198, 1202
BstMAI GTCTC 3 cut(s) 51, 443, 1120
BstMBI GATC 5 cut(s) 603, 988, 1061, 1195, 1199
BstMWI GCNNNNNNNGC 2 cut(s) 665, 842
BstNI CCWGG 1 cut(s) 1007
BstNSI RCATGY 2 cut(s) 475, 1005
BstPAI GACNNNNGTC 1 cut(s) 22
BstSCI CCNGG 2 cut(s) 403, 1005
BstSFI CTRYAG 2 cut(s) 453, 1091
BstUI CGCG 2 cut(s) 98, 1155
BstV1I GCAGC 3 cut(s) 854, 874, 1077
BstV2I GAAGAC 1 cut(s) 18
BstZ17I GTATAC 1 cut(s) 75
Bsu15I ATCGAT 1 cut(s) 1198
Bsu36I CCTNAGG 1 cut(s) 287
BsuI GTATCC 1 cut(s) 704
BsuRI GGCC 3 cut(s) 198, 594, 836
BsuTUI ATCGAT 1 cut(s) 1198
BtgI CCRYGG 2 cut(s) 156, 1153
BtsCI GGATG 4 cut(s) 90, 152, 550, 862
BtsI GCAGTG 2 cut(s) 244, 462
BtsIMutI CAGTG 2 cut(s) 244, 462
BveI ACCTGC 2 cut(s) 408, 836
CciI TCATGA 1 cut(s) 1192
Cfr13I GGNCC 2 cut(s) 197, 593
Cfr42I CCGCGG 1 cut(s) 1156
ClaI ATCGAT 1 cut(s) 1198
Csp6I GTAC 1 cut(s) 759
CviAII CATG 5 cut(s) 157, 472, 601, 1002, 1193
CviQI GTAC 1 cut(s) 759
DdeI CTNAG 2 cut(s) 287, 908
DpnI GATC 5 cut(s) 605, 990, 1063, 1197, 1201
DpnII GATC 5 cut(s) 603, 988, 1061, 1195, 1199
EaeI YGGCCR 1 cut(s) 834
Eam1104I CTCTTC 3 cut(s) 154, 301, 1220
EarI CTCTTC 3 cut(s) 154, 301, 1220
Eco130I CCWWGG 1 cut(s) 156
Eco57I CTGAAG 1 cut(s) 222
Eco81I CCTNAGG 1 cut(s) 287
EcoRII CCWGG 1 cut(s) 1005
EcoT14I CCWWGG 1 cut(s) 156
ErhI CCWWGG 1 cut(s) 156
FaeI CATG 5 cut(s) 160, 475, 604, 1005, 1196
FatI CATG 5 cut(s) 156, 471, 600, 1001, 1192
FauI CCCGC 2 cut(s) 509, 1148
FblI GTMKAC 1 cut(s) 74
Fnu4HI GCNGC 4 cut(s) 261, 843, 888, 1091
FokI GGATG 4 cut(s) 77, 139, 557, 869
Fsp4HI GCNGC 4 cut(s) 261, 843, 888, 1091
FspBI CTAG 1 cut(s) 1163
GluI GCNGC 4 cut(s) 261, 843, 888, 1091
GsaI CCCAGC 1 cut(s) 419
GsuI CTGGAG 1 cut(s) 147
HaeIII GGCC 3 cut(s) 198, 594, 836
HapII CCGG 1 cut(s) 405
Hin1II CATG 5 cut(s) 160, 475, 604, 1005, 1196
HindIII AAGCTT 1 cut(s) 1035
HinfI GANTC 2 cut(s) 222, 900
HpaII CCGG 1 cut(s) 405
HphI GGTGA 2 cut(s) 217, 362
Hpy166II GTNNAC 3 cut(s) 75, 460, 1135
Hpy188I TCNGA 1 cut(s) 651
Hpy188III TCNNGA 4 cut(s) 164, 583, 607, 1193
Hpy8I GTNNAC 3 cut(s) 75, 460, 1135
Hpy99I CGWCG 3 cut(s) 21, 233, 236
HpyAV CCTTC 7 cut(s) 65, 310, 526, 584, 791, 913, 973
HpyCH4III ACNGT 4 cut(s) 229, 464, 706, 1189
HpyCH4V TGCA 9 cut(s) 434, 455, 600, 734, 890, 1043, 1093, 1184, 1205
HpyF10VI GCNNNNNNNGC 2 cut(s) 665, 842
HpyF3I CTNAG 2 cut(s) 287, 908
Hsp92II CATG 5 cut(s) 160, 475, 604, 1005, 1196
KspI CCGCGG 1 cut(s) 1156
Kzo9I GATC 5 cut(s) 603, 988, 1061, 1195, 1199
LmnI GCTCC 4 cut(s) 71, 166, 211, 656
Lsp1109I GCAGC 3 cut(s) 854, 874, 1077
LweI GCATC 2 cut(s) 14, 355
MaeI CTAG 1 cut(s) 1163
MaeIII GTNAC 3 cut(s) 223, 484, 775
MalI GATC 5 cut(s) 605, 990, 1063, 1197, 1201
MboI GATC 5 cut(s) 603, 988, 1061, 1195, 1199
MboII GAAGA 8 cut(s) 18, 141, 194, 288, 958, 1026, 1162, 1207
MfeI CAATTG 1 cut(s) 626
MlsI TGGCCA 1 cut(s) 836
MluCI AATT 7 cut(s) 497, 555, 626, 637, 689, 891, 1103
MluI ACGCGT 1 cut(s) 96
MluNI TGGCCA 1 cut(s) 836
MlyI GAGTC 1 cut(s) 231
MmeI TCCRAC 4 cut(s) 196, 303, 688, 843
Mox20I TGGCCA 1 cut(s) 836
MscI TGGCCA 1 cut(s) 836
MseI TTAA 3 cut(s) 411, 636, 1145
MslI CAYNNNNRTG 2 cut(s) 476, 739
Msp20I TGGCCA 1 cut(s) 836
MspA1I CMGCKG 1 cut(s) 1155
MspI CCGG 1 cut(s) 405
MspR9I CCNGG 2 cut(s) 405, 1007
MunI CAATTG 1 cut(s) 626
MvaI CCWGG 1 cut(s) 1007
MvnI CGCG 2 cut(s) 98, 1155
MwoI GCNNNNNNNGC 2 cut(s) 665, 842
NciI CCSGG 1 cut(s) 405
NcoI CCATGG 1 cut(s) 156
NdeII GATC 5 cut(s) 603, 988, 1061, 1195, 1199
NlaIII CATG 5 cut(s) 160, 475, 604, 1005, 1196
NlaIV GGNNCC 2 cut(s) 162, 817
NmuCI GTSAC 3 cut(s) 223, 484, 775
NspI RCATGY 2 cut(s) 475, 1005
NspV TTCGAA 1 cut(s) 969
PagI TCATGA 1 cut(s) 1192
PfeI GAWTC 1 cut(s) 900
PflMI CCANNNNNTGG 1 cut(s) 793
PkrI GCNGC 4 cut(s) 262, 844, 889, 1092
PleI GAGTC 1 cut(s) 230
PpsI GAGTC 1 cut(s) 230
PshAI GACNNNNGTC 1 cut(s) 22
PshBI ATTAAT 1 cut(s) 636
Psp6I CCWGG 1 cut(s) 1005
PspFI CCCAGC 1 cut(s) 415
PspGI CCWGG 1 cut(s) 1005
PspN4I GGNNCC 2 cut(s) 162, 817
PspPI GGNCC 2 cut(s) 197, 593
PstI CTGCAG 2 cut(s) 457, 1095
RsaI GTAC 1 cut(s) 760
RsaNI GTAC 1 cut(s) 759
RseI CAYNNNNRTG 2 cut(s) 476, 739
SacII CCGCGG 1 cut(s) 1156
SaqAI TTAA 3 cut(s) 411, 636, 1145
SatI GCNGC 4 cut(s) 261, 843, 888, 1091
Sau3AI GATC 5 cut(s) 603, 988, 1061, 1195, 1199
Sau96I GGNCC 2 cut(s) 197, 593
ScaI AGTACT 1 cut(s) 760
SchI GAGTC 1 cut(s) 231
ScrFI CCNGG 2 cut(s) 405, 1007
SfaNI GCATC 2 cut(s) 14, 355
SfcI CTRYAG 2 cut(s) 453, 1091
Sfr303I CCGCGG 1 cut(s) 1156
SfuI TTCGAA 1 cut(s) 969
SgrBI CCGCGG 1 cut(s) 1156
SmiMI CAYNNNNRTG 2 cut(s) 476, 739
SmlI CTYRAG 1 cut(s) 446
SmoI CTYRAG 1 cut(s) 446
Sse9I AATT 7 cut(s) 497, 555, 626, 637, 689, 891, 1103
SsiI CCGC 9 cut(s) 11, 261, 376, 516, 542, 1031, 1122, 1153, 1155
SspMI CTAG 1 cut(s) 1163
StyD4I CCNGG 2 cut(s) 403, 1005
StyI CCWWGG 1 cut(s) 156
TaaI ACNGT 4 cut(s) 229, 464, 706, 1189
TaqI TCGA 3 cut(s) 608, 969, 1198
TaqII GACCGA 1 cut(s) 893
TasI AATT 7 cut(s) 497, 555, 626, 637, 689, 891, 1103
TatI WGTACW 1 cut(s) 758
TauI GCSGC 1 cut(s) 263
TfiI GAWTC 1 cut(s) 900
Tru1I TTAA 3 cut(s) 411, 636, 1145
Tru9I TTAA 3 cut(s) 411, 636, 1145
TscAI CASTG 2 cut(s) 244, 462
TseFI GTSAC 3 cut(s) 223, 484, 775
TseI GCWGC 3 cut(s) 842, 887, 1090
Tsp45I GTSAC 3 cut(s) 223, 484, 775
TspDTI ATGAA 7 cut(s) 141, 324, 399, 760, 932, 933, 1181
TspRI CASTG 2 cut(s) 244, 462
Van91I CCANNNNNTGG 1 cut(s) 793
VspI ATTAAT 1 cut(s) 636
XapI RAATTY 1 cut(s) 689
XceI RCATGY 2 cut(s) 475, 1005
XmiI GTMKAC 1 cut(s) 74
XspI CTAG 1 cut(s) 1163
ZrmI AGTACT 1 cut(s) 760
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.