pycom17g10810

Nitrile-specifier protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0005031
Physical Location & Seq
Reverse (-)
8392563 .. 8393913
1351 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 456 bp
ATGTCTCTGGTGCAAGGCAAATGGATCAAGCTTGATCAAAAGGGGACTGGTCCTGGAGCAAGAAGCTCACATGCCATTACCCTAGTAGGACAGAAGGCCTATGTTTTCGGAGGCGAATTCACGCCACGTGTCCCCGTCGACAACAAGCTCCACGTGTTTGACCTCAAGGAGTTGACGTGGTCCGTGATCGAAGGAACCGGGGATGTTCCACCGCCACGTGTTGGTGTGACATTGGTAGCTGTTGGAGAAATTATTTATGTATTTGGAGGTAGGGATTATGAACACAATGAACTCAATGAGCTCTATTCCTTTGACACATCCACAAACAAGTGGACTCTAATTTCAAGTGGGGACACCGGGCCCCCTCACCGGAGTTACCACTCAGTCACCTCCAATGACCACCATGTATACATATTCGGTGGATGTGGTGTCGCCGGCCGTCTCAATGATCGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Kelch_1 Kelch_3 Kelch_4

Protein Analysis

152

Amino Acids

16.54

Weight (kDa)

6.36

Isoelectric Point (pI)

23.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Kelch_KLHDC2_KLHL20_DRC7 PF24681 4 - 38 4.3e-06 KLHDC2/KLHL20/DRC7 Kelch-repeats domain
Beta-prop_ATRN-LZTR1 PF24981 4 - 81 2.2e-10 Attractin/LZTR1 beta-propeller
Beta-prop_FBX42 PF13415 5 - 147 1.2e-28 FBX42, beta-propeller domain
Beta-prop_Calicin PF13964 7 - 145 1.8e-09 Calicin, beta-propeller domain
Kelch_KLHDC2_KLHL20_DRC7 PF24681 23 - 150 3.4e-43 KLHDC2/KLHL20/DRC7 Kelch-repeats domain
Kelch_HCF PF13854 62 - 147 3.6e-08 Host cell factor, Kelch-repeats domain
Kelch_FKB95 PF25210 70 - 143 2.9e-06 FKB95, Kelch-repeats domain
Kelch_1 PF01344 72 - 116 3.4e-12 Kelch motif
Beta-prop_ATRN-LZTR1 PF24981 85 - 142 4e-11 Attractin/LZTR1 beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 221
AccI GTMKAC 2 cut(s) 138, 408
AciI CCGC 1 cut(s) 212
AclWI GGATC 1 cut(s) 32
AcoI YGGCCR 1 cut(s) 436
AcsI RAATTY 1 cut(s) 116
AcvI CACGTG 3 cut(s) 128, 154, 218
AfiI CCNNNNNNNGG 2 cut(s) 221, 369
AflIII ACRYGT 3 cut(s) 127, 153, 217
AgsI TTSAA 1 cut(s) 345
AjiI CACGTC 1 cut(s) 177
AjnI CCWGG 1 cut(s) 52
AluBI AGCT 5 cut(s) 31, 66, 148, 239, 301
AluI AGCT 5 cut(s) 31, 66, 148, 239, 301
Alw21I GWGCWC 1 cut(s) 303
Alw26I GTCTC 2 cut(s) 9, 446
AlwI GGATC 1 cut(s) 32
AoxI GGCC 3 cut(s) 96, 359, 436
ApaI GGGCCC 1 cut(s) 363
ApoI RAATTY 1 cut(s) 116
AspS9I GGNCC 4 cut(s) 50, 180, 359, 360
AsuC2I CCSGG 2 cut(s) 199, 358
AsuHPI GGTGA 2 cut(s) 359, 379
AvaII GGWCC 2 cut(s) 50, 180
BaeGI GKGCMC 1 cut(s) 363
BanII GRGCYC 2 cut(s) 303, 363
BbrPI CACGTG 3 cut(s) 128, 154, 218
Bbv12I GWGCWC 1 cut(s) 303
BceAI ACGGC 1 cut(s) 423
BciT130I CCWGG 1 cut(s) 54
BclI TGATCA 1 cut(s) 34
BcnI CCSGG 2 cut(s) 199, 358
BcoDI GTCTC 2 cut(s) 9, 446
BfaI CTAG 1 cut(s) 83
Bme1390I CCNGG 3 cut(s) 54, 199, 358
Bme18I GGWCC 2 cut(s) 50, 180
BmgBI CACGTC 1 cut(s) 177
BmgT120I GGNCC 4 cut(s) 50, 180, 359, 360
BmiI GGNNCC 3 cut(s) 196, 361, 362
BmrFI CCNGG 3 cut(s) 54, 199, 358
BpmI CTGGAG 1 cut(s) 75
BpuEI CTTGAG 1 cut(s) 149
BpuMI CCSGG 2 cut(s) 199, 358
BsaAI YACGTR 3 cut(s) 128, 154, 218
BsaJI CCNNGG 1 cut(s) 198
BsaWI WCCGGW 1 cut(s) 369
BsaXI ACNNNNNCTCC 2 cut(s) 161, 191
Bsc4I CCNNNNNNNGG 2 cut(s) 221, 369
Bse118I RCCGGY 1 cut(s) 434
Bse1I ACTGG 1 cut(s) 52
BseBI CCWGG 1 cut(s) 54
BseDI CCNNGG 1 cut(s) 198
BseGI GGATG 3 cut(s) 208, 317, 428
BseLI CCNNNNNNNGG 2 cut(s) 221, 369
BseMII CTCAG 1 cut(s) 396
BseNI ACTGG 1 cut(s) 52
BseSI GKGCMC 1 cut(s) 363
BseX3I CGGCCG 1 cut(s) 436
Bsh1285I CGRYCG 1 cut(s) 439
BshFI GGCC 3 cut(s) 98, 361, 438
BsiEI CGRYCG 1 cut(s) 439
BsiHKAI GWGCWC 1 cut(s) 303
BsiSI CCGG 4 cut(s) 198, 357, 370, 435
BslFI GGGAC 3 cut(s) 58, 116, 365
BslI CCNNNNNNNGG 2 cut(s) 221, 369
BsmAI GTCTC 2 cut(s) 9, 446
BsmBI CGTCTC 1 cut(s) 446
BsmFI GGGAC 3 cut(s) 58, 116, 365
BsnI GGCC 3 cut(s) 98, 361, 438
Bsp120I GGGCCC 1 cut(s) 359
Bsp1286I GDGCHC 2 cut(s) 303, 363
Bsp143I GATC 4 cut(s) 24, 34, 186, 448
BspACI CCGC 1 cut(s) 212
BspANI GGCC 3 cut(s) 98, 361, 438
BspCNI CTCAG 1 cut(s) 395
BspLI GGNNCC 3 cut(s) 196, 361, 362
BspPI GGATC 1 cut(s) 32
BsrFI RCCGGY 1 cut(s) 434
BsrI ACTGG 1 cut(s) 52
BssAI RCCGGY 1 cut(s) 434
BssECI CCNNGG 1 cut(s) 198
BssMI GATC 4 cut(s) 24, 34, 186, 448
BssNAI GTATAC 1 cut(s) 409
Bst1107I GTATAC 1 cut(s) 409
Bst2UI CCWGG 1 cut(s) 54
BstBAI YACGTR 3 cut(s) 128, 154, 218
BstC8I GCNNGC 1 cut(s) 436
BstDEI CTNAG 1 cut(s) 382
BstF5I GGATG 3 cut(s) 208, 317, 428
BstKTI GATC 4 cut(s) 27, 37, 189, 451
BstMAI GTCTC 2 cut(s) 9, 446
BstMBI GATC 4 cut(s) 24, 34, 186, 448
BstMCI CGRYCG 1 cut(s) 439
BstNI CCWGG 1 cut(s) 54
BstNSI RCATGY 1 cut(s) 74
BstSCI CCNGG 3 cut(s) 52, 197, 356
BstSLI GKGCMC 1 cut(s) 363
BstZ17I GTATAC 1 cut(s) 409
BstZI CGGCCG 1 cut(s) 436
BsuRI GGCC 3 cut(s) 98, 361, 438
BtrI CACGTC 1 cut(s) 177
BtsCI GGATG 3 cut(s) 208, 317, 428
Cac8I GCNNGC 1 cut(s) 436
Cfr10I RCCGGY 1 cut(s) 434
Cfr13I GGNCC 4 cut(s) 50, 180, 359, 360
CviAII CATG 2 cut(s) 71, 404
CviJI RGCY 8 cut(s) 31, 66, 98, 148, 239, 301, 361, 438
CviKI_1 RGCY 8 cut(s) 31, 66, 98, 148, 239, 301, 361, 438
DdeI CTNAG 1 cut(s) 382
DpnI GATC 4 cut(s) 26, 36, 188, 450
DpnII GATC 4 cut(s) 24, 34, 186, 448
EaeI YGGCCR 1 cut(s) 436
EagI CGGCCG 1 cut(s) 436
Ecl136II GAGCTC 1 cut(s) 301
EclXI CGGCCG 1 cut(s) 436
Eco147I AGGCCT 1 cut(s) 98
Eco24I GRGCYC 2 cut(s) 303, 363
Eco47I GGWCC 2 cut(s) 50, 180
Eco52I CGGCCG 1 cut(s) 436
Eco53kI GAGCTC 1 cut(s) 301
Eco72I CACGTG 3 cut(s) 128, 154, 218
EcoICRI GAGCTC 1 cut(s) 301
EcoO109I RGGNCCY 1 cut(s) 360
EcoRI GAATTC 1 cut(s) 116
EcoRII CCWGG 1 cut(s) 52
EcoT38I GRGCYC 2 cut(s) 303, 363
Esp3I CGTCTC 1 cut(s) 446
FaeI CATG 2 cut(s) 74, 407
FaiI YATR 7 cut(s) 72, 102, 258, 279, 405, 409, 413
FaqI GGGAC 3 cut(s) 58, 116, 365
FatI CATG 2 cut(s) 70, 403
FbaI TGATCA 1 cut(s) 34
FblI GTMKAC 2 cut(s) 138, 408
FokI GGATG 3 cut(s) 215, 304, 435
FriOI GRGCYC 2 cut(s) 303, 363
FspBI CTAG 1 cut(s) 83
GsuI CTGGAG 1 cut(s) 75
HaeIII GGCC 3 cut(s) 98, 361, 438
HapII CCGG 4 cut(s) 198, 357, 370, 435
Hin1II CATG 2 cut(s) 74, 407
HincII GTYRAC 2 cut(s) 139, 174
HindII GTYRAC 2 cut(s) 139, 174
HindIII AAGCTT 1 cut(s) 29
HinfI GANTC 1 cut(s) 334
HpaII CCGG 4 cut(s) 198, 357, 370, 435
HphI GGTGA 2 cut(s) 359, 379
Hpy166II GTNNAC 4 cut(s) 139, 174, 333, 409
Hpy188I TCNGA 1 cut(s) 110
Hpy8I GTNNAC 4 cut(s) 139, 174, 333, 409
Hpy99I CGWCG 1 cut(s) 140
HpyAV CCTTC 2 cut(s) 88, 185
HpyCH4IV ACGT 4 cut(s) 127, 153, 176, 217
HpyCH4V TGCA 1 cut(s) 13
HpyF3I CTNAG 1 cut(s) 382
HpySE526I ACGT 4 cut(s) 127, 153, 176, 217
Hsp92II CATG 2 cut(s) 74, 407
KroI GCCGGC 1 cut(s) 434
KroNI GCCGGC 1 cut(s) 436
Ksp22I TGATCA 1 cut(s) 34
Kzo9I GATC 4 cut(s) 24, 34, 186, 448
LmnI GCTCC 2 cut(s) 56, 153
LpnPI CCDG 7 cut(s) 33, 39, 66, 211, 370, 383, 448
MaeI CTAG 1 cut(s) 83
MaeII ACGT 4 cut(s) 127, 153, 176, 217
MaeIII GTNAC 3 cut(s) 226, 374, 385
MalI GATC 4 cut(s) 26, 36, 188, 450
MboI GATC 4 cut(s) 24, 34, 186, 448
MhlI GDGCHC 2 cut(s) 303, 363
MluCI AATT 3 cut(s) 116, 249, 339
MlyI GAGTC 1 cut(s) 328
MmeI TCCRAC 1 cut(s) 223
MnlI CCTC 5 cut(s) 104, 173, 260, 375, 400
MroNI GCCGGC 1 cut(s) 434
MspI CCGG 4 cut(s) 198, 357, 370, 435
MspR9I CCNGG 3 cut(s) 54, 199, 358
MvaI CCWGG 1 cut(s) 54
NaeI GCCGGC 1 cut(s) 436
NciI CCSGG 2 cut(s) 199, 358
NdeII GATC 4 cut(s) 24, 34, 186, 448
NgoMIV GCCGGC 1 cut(s) 434
NlaIII CATG 2 cut(s) 74, 407
NlaIV GGNNCC 3 cut(s) 196, 361, 362
NmuCI GTSAC 2 cut(s) 226, 385
NspI RCATGY 1 cut(s) 74
PceI AGGCCT 1 cut(s) 98
PdiI GCCGGC 1 cut(s) 436
PflFI GACNNNGTC 1 cut(s) 178
PflMI CCANNNNNTGG 1 cut(s) 221
PfoI TCCNGGA 1 cut(s) 52
PleI GAGTC 1 cut(s) 328
PmaCI CACGTG 3 cut(s) 128, 154, 218
PmlI CACGTG 3 cut(s) 128, 154, 218
PpsI GAGTC 1 cut(s) 328
Ppu21I YACGTR 3 cut(s) 128, 154, 218
Psp124BI GAGCTC 1 cut(s) 303
Psp6I CCWGG 1 cut(s) 52
PspCI CACGTG 3 cut(s) 128, 154, 218
PspGI CCWGG 1 cut(s) 52
PspN4I GGNNCC 3 cut(s) 196, 361, 362
PspOMI GGGCCC 1 cut(s) 359
PspPI GGNCC 4 cut(s) 50, 180, 359, 360
PsyI GACNNNGTC 1 cut(s) 178
SacI GAGCTC 1 cut(s) 303
SalI GTCGAC 1 cut(s) 137
Sau3AI GATC 4 cut(s) 24, 34, 186, 448
Sau96I GGNCC 4 cut(s) 50, 180, 359, 360
SchI GAGTC 1 cut(s) 328
ScrFI CCNGG 3 cut(s) 54, 199, 358
SduI GDGCHC 2 cut(s) 303, 363
SinI GGWCC 2 cut(s) 50, 180
SmlI CTYRAG 1 cut(s) 164
SmoI CTYRAG 1 cut(s) 164
Sse9I AATT 3 cut(s) 116, 249, 339
SseBI AGGCCT 1 cut(s) 98
SsiI CCGC 1 cut(s) 212
SspMI CTAG 1 cut(s) 83
SstI GAGCTC 1 cut(s) 303
StuI AGGCCT 1 cut(s) 98
StyD4I CCNGG 3 cut(s) 52, 197, 356
TaiI ACGT 4 cut(s) 130, 156, 179, 220
TaqI TCGA 2 cut(s) 138, 189
TasI AATT 3 cut(s) 116, 249, 339
TseFI GTSAC 2 cut(s) 226, 385
Tsp45I GTSAC 2 cut(s) 226, 385
TspDTI ATGAA 2 cut(s) 294, 303
TspGWI ACGGA 1 cut(s) 172
Tth111I GACNNNGTC 1 cut(s) 178
Van91I CCANNNNNTGG 1 cut(s) 221
VpaK11BI GGWCC 2 cut(s) 50, 180
XapI RAATTY 1 cut(s) 116
XceI RCATGY 1 cut(s) 74
XmiI GTMKAC 2 cut(s) 138, 408
XspI CTAG 1 cut(s) 83
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.