pycom17g14200

chromatin binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0007403
Physical Location & Seq
Forward (+)
11753740 .. 11755802
2063 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 546 bp
ATGGGTGTTTCGTTTAAGGTTTCCAAGACCGGTACTAGGTTCCGTCCGAAGCCGCCTCTCCAGTCCGAGGCCGACGTTGGCGGCGATGACGTGTCCAAGGCTCCCAACAACAGCAGCTCCCGAGCTGTCCCCAGAAAGCTCGAGGGTGAGAGTGGGGCCGGGGTTTCTGGTCCATCAATGTCTTCTGAAGAGTTTCTTGTATCTGCAGGTTCGTCACAATTTTTTAGGGGGTTGGGGATTAGCCTTAGTTTTATCTTGATTTTTCTTCCCTTTGTTTTGACAGAAAACGAAGTTTCCTTCACTTTGAATCTCTTCCCAGATGGATATTCTTTTGGAAAACCATCAGAGAATGACACTGCGCATCAAGCTACACATCAAGATGTTCCAAAGTTGTTACACCCATATGATAGGACGTCAGAAACTCTTTTTTCGGCAATTGAATCTGGCCGGTTGCCTGGAGATATTCTAGATGATATACCTTGCAAGTATGTCGATGGAACACTTGTTTGTGAGATTCGTGATTATCGCAAATGTGCTTTTGAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

182

Amino Acids

19.66

Weight (kDa)

5.12

Isoelectric Point (pI)

38.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Spt20_SEP PF12090 96 - 177 2.9e-12 Spt20, SEP domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 416
Acc16I TGCGCA 1 cut(s) 360
Acc36I ACCTGC 1 cut(s) 197
AciI CCGC 2 cut(s) 53, 81
AcoI YGGCCR 1 cut(s) 445
AcuI CTGAAG 1 cut(s) 207
AcyI GRCGYC 1 cut(s) 413
AfaI GTAC 1 cut(s) 34
AfiI CCNNNNNNNGG 2 cut(s) 36, 67
AflIII ACRYGT 1 cut(s) 90
AgeI ACCGGT 1 cut(s) 29
AgsI TTSAA 3 cut(s) 307, 440, 542
AjiI CACGTC 1 cut(s) 91
AjnI CCWGG 1 cut(s) 454
AluBI AGCT 4 cut(s) 117, 125, 139, 368
AluI AGCT 4 cut(s) 117, 125, 139, 368
Ama87I CYCGRG 2 cut(s) 120, 140
AoxI GGCC 3 cut(s) 69, 156, 445
ApeKI GCWGC 1 cut(s) 114
AsiGI ACCGGT 1 cut(s) 29
Asp700I GAANNNNTTC 2 cut(s) 192, 311
AspLEI GCGC 1 cut(s) 361
AspS9I GGNCC 2 cut(s) 156, 170
AsuC2I CCSGG 1 cut(s) 160
AsuHPI GGTGA 1 cut(s) 158
AvaI CYCGRG 2 cut(s) 120, 140
AvaII GGWCC 1 cut(s) 170
BbsI GAAGAC 1 cut(s) 174
BbvI GCAGC 1 cut(s) 126
BccI CCATC 4 cut(s) 181, 314, 349, 488
BcgI CGANNNNNNTGC 2 cut(s) 472, 506
BciT130I CCWGG 1 cut(s) 456
BcnI CCSGG 1 cut(s) 160
BfaI CTAG 2 cut(s) 36, 467
BfmI CTRYAG 1 cut(s) 204
BfuAI ACCTGC 1 cut(s) 197
BisI GCNGC 3 cut(s) 53, 82, 115
BlsI GCNGC 3 cut(s) 54, 83, 116
Bme1390I CCNGG 2 cut(s) 160, 456
Bme18I GGWCC 1 cut(s) 170
BmeT110I CYCGRG 2 cut(s) 120, 140
BmgBI CACGTC 1 cut(s) 91
BmgT120I GGNCC 2 cut(s) 156, 170
BmiI GGNNCC 3 cut(s) 41, 102, 157
BmrFI CCNGG 2 cut(s) 160, 456
BmsI GCATC 1 cut(s) 370
BpiI GAAGAC 1 cut(s) 174
BpmI CTGGAG 2 cut(s) 44, 477
BpuMI CCSGG 1 cut(s) 160
BsaHI GRCGYC 1 cut(s) 413
BsaJI CCNNGG 3 cut(s) 66, 96, 159
BsaWI WCCGGW 1 cut(s) 29
BsaXI ACNNNNNCTCC 2 cut(s) 101, 131
Bsc4I CCNNNNNNNGG 2 cut(s) 36, 67
Bse118I RCCGGY 2 cut(s) 29, 447
Bse1I ACTGG 1 cut(s) 61
BseBI CCWGG 1 cut(s) 456
BseDI CCNNGG 3 cut(s) 66, 96, 159
BseLI CCNNNNNNNGG 2 cut(s) 36, 67
BseNI ACTGG 1 cut(s) 61
BseXI GCAGC 1 cut(s) 126
BshFI GGCC 3 cut(s) 71, 158, 447
BshTI ACCGGT 1 cut(s) 29
BsiHKCI CYCGRG 2 cut(s) 120, 140
BsiSI CCGG 3 cut(s) 30, 159, 448
BslFI GGGAC 1 cut(s) 113
BslI CCNNNNNNNGG 2 cut(s) 36, 67
BsmFI GGGAC 1 cut(s) 113
BsnI GGCC 3 cut(s) 71, 158, 447
BsoBI CYCGRG 2 cut(s) 120, 140
BspACI CCGC 2 cut(s) 53, 81
BspANI GGCC 3 cut(s) 71, 158, 447
BspLI GGNNCC 3 cut(s) 41, 102, 157
BspMAI CTGCAG 1 cut(s) 208
BspMI ACCTGC 1 cut(s) 197
BsrFI RCCGGY 2 cut(s) 29, 447
BsrI ACTGG 1 cut(s) 61
BssAI RCCGGY 2 cut(s) 29, 447
BssECI CCNNGG 3 cut(s) 66, 96, 159
BssNI GRCGYC 1 cut(s) 413
BssT1I CCWWGG 1 cut(s) 96
Bst2UI CCWGG 1 cut(s) 456
Bst6I CTCTTC 2 cut(s) 183, 317
BstACI GRCGYC 1 cut(s) 413
BstDEI CTNAG 1 cut(s) 245
BstHHI GCGC 1 cut(s) 361
BstMWI GCNNNNNNNGC 1 cut(s) 365
BstNI CCWGG 1 cut(s) 456
BstSCI CCNGG 2 cut(s) 158, 454
BstSFI CTRYAG 1 cut(s) 204
BstV1I GCAGC 1 cut(s) 126
BstV2I GAAGAC 1 cut(s) 174
BsuRI GGCC 3 cut(s) 71, 158, 447
BtgZI GCGATG 1 cut(s) 99
BtrI CACGTC 1 cut(s) 91
BtsI GCAGTG 1 cut(s) 354
BtsIMutI CAGTG 1 cut(s) 354
BveI ACCTGC 1 cut(s) 197
CfoI GCGC 1 cut(s) 361
Cfr10I RCCGGY 2 cut(s) 29, 447
Cfr13I GGNCC 2 cut(s) 156, 170
Csp6I GTAC 1 cut(s) 33
CspAI ACCGGT 1 cut(s) 29
CviQI GTAC 1 cut(s) 33
DdeI CTNAG 1 cut(s) 245
EaeI YGGCCR 1 cut(s) 445
Eam1104I CTCTTC 2 cut(s) 183, 317
EarI CTCTTC 2 cut(s) 183, 317
Eco130I CCWWGG 1 cut(s) 96
Eco47I GGWCC 1 cut(s) 170
Eco57I CTGAAG 1 cut(s) 207
Eco88I CYCGRG 2 cut(s) 120, 140
EcoRII CCWGG 1 cut(s) 454
EcoT14I CCWWGG 1 cut(s) 96
ErhI CCWWGG 1 cut(s) 96
FaiI YATR 4 cut(s) 403, 405, 476, 489
FalI AAGNNNNNCTT 2 cut(s) 180, 212
FaqI GGGAC 1 cut(s) 113
FauNDI CATATG 1 cut(s) 403
Fnu4HI GCNGC 3 cut(s) 53, 82, 115
Fsp4HI GCNGC 3 cut(s) 53, 82, 115
FspBI CTAG 2 cut(s) 36, 467
FspI TGCGCA 1 cut(s) 360
GlaI GCGC 1 cut(s) 360
GluI GCNGC 3 cut(s) 53, 82, 115
GsuI CTGGAG 2 cut(s) 44, 477
HaeIII GGCC 3 cut(s) 71, 158, 447
HapII CCGG 3 cut(s) 30, 159, 448
HhaI GCGC 1 cut(s) 361
Hin1I GRCGYC 1 cut(s) 413
Hin6I GCGC 1 cut(s) 359
HinP1I GCGC 1 cut(s) 359
HinfI GANTC 3 cut(s) 307, 440, 514
HpaII CCGG 3 cut(s) 30, 159, 448
HphI GGTGA 1 cut(s) 158
Hpy188I TCNGA 5 cut(s) 48, 67, 187, 346, 418
Hpy188III TCNNGA 5 cut(s) 120, 256, 377, 467, 518
Hpy99I CGWCG 1 cut(s) 77
HpyAV CCTTC 1 cut(s) 307
HpyCH4IV ACGT 3 cut(s) 75, 90, 413
HpyCH4V TGCA 2 cut(s) 206, 483
HpyF10VI GCNNNNNNNGC 1 cut(s) 365
HpyF3I CTNAG 1 cut(s) 245
HpySE526I ACGT 3 cut(s) 75, 90, 413
Hsp92I GRCGYC 1 cut(s) 413
HspAI GCGC 1 cut(s) 359
LmnI GCTCC 2 cut(s) 106, 122
Lsp1109I GCAGC 1 cut(s) 126
LweI GCATC 1 cut(s) 370
MaeI CTAG 2 cut(s) 36, 467
MaeII ACGT 3 cut(s) 75, 90, 413
MaeIII GTNAC 2 cut(s) 213, 393
MboII GAAGA 4 cut(s) 174, 200, 257, 304
MfeI CAATTG 1 cut(s) 435
MluCI AATT 2 cut(s) 218, 435
MnlI CCTC 3 cut(s) 61, 66, 136
MroXI GAANNNNTTC 2 cut(s) 192, 311
MseI TTAA 1 cut(s) 15
MslI CAYNNNNRTG 2 cut(s) 378, 402
MspI CCGG 3 cut(s) 30, 159, 448
MspR9I CCNGG 2 cut(s) 160, 456
MunI CAATTG 1 cut(s) 435
MvaI CCWGG 1 cut(s) 456
MwoI GCNNNNNNNGC 1 cut(s) 365
NciI CCSGG 1 cut(s) 160
NdeI CATATG 1 cut(s) 403
NlaIV GGNNCC 3 cut(s) 41, 102, 157
NmuCI GTSAC 1 cut(s) 213
NsbI TGCGCA 1 cut(s) 360
PaeR7I CTCGAG 1 cut(s) 140
PcsI WCGNNNNNNNCGW 1 cut(s) 81
PdmI GAANNNNTTC 2 cut(s) 192, 311
PfeI GAWTC 3 cut(s) 307, 440, 514
PinAI ACCGGT 1 cut(s) 29
PkrI GCNGC 3 cut(s) 54, 83, 116
Psp6I CCWGG 1 cut(s) 454
PspGI CCWGG 1 cut(s) 454
PspN4I GGNNCC 3 cut(s) 41, 102, 157
PspPI GGNCC 2 cut(s) 156, 170
PspXI VCTCGAGB 1 cut(s) 140
PstI CTGCAG 1 cut(s) 208
RsaI GTAC 1 cut(s) 34
RsaNI GTAC 1 cut(s) 33
RseI CAYNNNNRTG 2 cut(s) 378, 402
SaqAI TTAA 1 cut(s) 15
SatI GCNGC 3 cut(s) 53, 82, 115
Sau96I GGNCC 2 cut(s) 156, 170
ScrFI CCNGG 2 cut(s) 160, 456
SfaNI GCATC 1 cut(s) 370
SfcI CTRYAG 1 cut(s) 204
Sfr274I CTCGAG 1 cut(s) 140
SinI GGWCC 1 cut(s) 170
SlaI CTCGAG 1 cut(s) 140
SmiMI CAYNNNNRTG 2 cut(s) 378, 402
SmlI CTYRAG 1 cut(s) 140
SmoI CTYRAG 1 cut(s) 140
Sse9I AATT 2 cut(s) 218, 435
SsiI CCGC 2 cut(s) 53, 81
SspMI CTAG 2 cut(s) 36, 467
StyD4I CCNGG 2 cut(s) 158, 454
StyI CCWWGG 1 cut(s) 96
TaiI ACGT 3 cut(s) 78, 93, 416
TaqI TCGA 2 cut(s) 141, 492
TasI AATT 2 cut(s) 218, 435
TauI GCSGC 2 cut(s) 55, 84
TfiI GAWTC 3 cut(s) 307, 440, 514
Tru1I TTAA 1 cut(s) 15
Tru9I TTAA 1 cut(s) 15
TscAI CASTG 1 cut(s) 361
TseFI GTSAC 1 cut(s) 213
TseI GCWGC 1 cut(s) 114
Tsp45I GTSAC 1 cut(s) 213
TspGWI ACGGA 1 cut(s) 32
TspRI CASTG 1 cut(s) 361
VpaK11BI GGWCC 1 cut(s) 170
XbaI TCTAGA 1 cut(s) 466
XhoI CTCGAG 1 cut(s) 140
XmnI GAANNNNTTC 2 cut(s) 192, 311
XspI CTAG 2 cut(s) 36, 467
ZraI GACGTC 1 cut(s) 414
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.