pycom17g14330

2Fe-2S iron-sulfur cluster binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0009702
Physical Location & Seq
Reverse (-)
11827526 .. 11829442
1917 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 552 bp
ATGCTTGTTTCTAGACTATCCGCGGTTAGAACTCAGATGATCAAGCATCTCGTAAAAGCTCCATTGACATCTGCACCAAAGACAGGAAACTTCAGTGGACGTTACAGTTATATTTTCCACAATCCGAAGCATCAAACTTACTCTTCGACAACTGCTCATGAAGAGGGGGATAAAAAAGTAGAAGAAATTTCTGTCACATTTGTTGACAAGGATGAGATGGAAAAGCATATTAAGATTCCCGTTGGAATGTCCATACTAGAAGCTGCTCATGAAAATGACATAGAACTTGAAGGAGCATGTGAAGGATCGTGCGCTTGCTCAACATGTCATGTGATTGTGAGAGTATGGAATACTACAATAAACTCGAAGATCCATCTTATGAGGAAAACGACATGCTGGATCTTGCTTTCGGGCTCACAGAGACGTCCCGTCTGGGTTGTCAAATGGTTGCAAAACCCGAACTTGACGGAATATGCCTTGAACTGCCGAGTGCAACAGGCAACTTTGCTGTTGATAGGTATGCCAAAGCCACATAGAATAGAATTCTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Fer2

Protein Analysis

184

Amino Acids

20.82

Weight (kDa)

8.92

Isoelectric Point (pI)

46.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Fer2 PF00111 72 - 114 9.3e-08 2Fe-2S iron-sulfur cluster binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 427
AccII CGCG 1 cut(s) 23
AciI CCGC 2 cut(s) 21, 23
AclWI GGATC 3 cut(s) 313, 364, 407
AcsI RAATTY 2 cut(s) 186, 542
AcuI CTGAAG 1 cut(s) 76
AcyI GRCGYC 1 cut(s) 424
AfiI CCNNNNNNNGG 1 cut(s) 83
AflIII ACRYGT 1 cut(s) 323
AgsI TTSAA 2 cut(s) 290, 481
AhdI GACNNNNNGTC 1 cut(s) 428
AluBI AGCT 2 cut(s) 59, 263
AluI AGCT 2 cut(s) 59, 263
Alw26I GTCTC 1 cut(s) 415
AlwI GGATC 3 cut(s) 313, 364, 407
ApeKI GCWGC 1 cut(s) 263
ApoI RAATTY 2 cut(s) 186, 542
AspLEI GCGC 1 cut(s) 314
BanII GRGCYC 1 cut(s) 416
BbvI GCAGC 1 cut(s) 250
BccI CCATC 2 cut(s) 211, 381
BclI TGATCA 1 cut(s) 39
BcoDI GTCTC 1 cut(s) 415
BfaI CTAG 2 cut(s) 12, 257
BisI GCNGC 1 cut(s) 264
BlsI GCNGC 1 cut(s) 265
BmeRI GACNNNNNGTC 1 cut(s) 428
BmsI GCATC 2 cut(s) 55, 139
BsaHI GRCGYC 1 cut(s) 424
BsaJI CCNNGG 1 cut(s) 21
Bsc4I CCNNNNNNNGG 1 cut(s) 83
BseDI CCNNGG 1 cut(s) 21
BseGI GGATG 1 cut(s) 217
BseLI CCNNNNNNNGG 1 cut(s) 83
BseMII CTCAG 1 cut(s) 47
BseXI GCAGC 1 cut(s) 250
BsgI GTGCAG 1 cut(s) 57
Bsh1236I CGCG 1 cut(s) 23
BslFI GGGAC 1 cut(s) 411
BslI CCNNNNNNNGG 1 cut(s) 83
BsmAI GTCTC 1 cut(s) 415
BsmBI CGTCTC 1 cut(s) 415
BsmFI GGGAC 1 cut(s) 411
Bsp1286I GDGCHC 1 cut(s) 416
Bsp143I GATC 4 cut(s) 39, 305, 369, 399
BspACI CCGC 2 cut(s) 21, 23
BspCNI CTCAG 1 cut(s) 46
BspFNI CGCG 1 cut(s) 23
BspHI TCATGA 2 cut(s) 157, 268
BspPI GGATC 3 cut(s) 313, 364, 407
BssECI CCNNGG 1 cut(s) 21
BssMI GATC 4 cut(s) 39, 305, 369, 399
BssNI GRCGYC 1 cut(s) 424
Bst4CI ACNGT 1 cut(s) 107
Bst6I CTCTTC 2 cut(s) 148, 156
BstACI GRCGYC 1 cut(s) 424
BstC8I GCNNGC 1 cut(s) 316
BstDEI CTNAG 1 cut(s) 33
BstDSI CCRYGG 1 cut(s) 21
BstF5I GGATG 1 cut(s) 217
BstFNI CGCG 1 cut(s) 23
BstHHI GCGC 1 cut(s) 314
BstKTI GATC 4 cut(s) 42, 308, 372, 402
BstMAI GTCTC 1 cut(s) 415
BstMBI GATC 4 cut(s) 39, 305, 369, 399
BstNSI RCATGY 3 cut(s) 300, 327, 396
BstUI CGCG 1 cut(s) 23
BstV1I GCAGC 1 cut(s) 250
BstX2I RGATCY 2 cut(s) 369, 399
BstYI RGATCY 2 cut(s) 369, 399
BtgI CCRYGG 1 cut(s) 21
BtsCI GGATG 1 cut(s) 217
BtsIMutI CAGTG 1 cut(s) 100
Cac8I GCNNGC 1 cut(s) 316
CciI TCATGA 2 cut(s) 157, 268
CfoI GCGC 1 cut(s) 314
Cfr42I CCGCGG 1 cut(s) 24
CviAII CATG 6 cut(s) 158, 269, 297, 324, 329, 393
CviJI RGCY 4 cut(s) 59, 263, 414, 529
CviKI_1 RGCY 4 cut(s) 59, 263, 414, 529
DdeI CTNAG 1 cut(s) 33
DpnI GATC 4 cut(s) 41, 307, 371, 401
DpnII GATC 4 cut(s) 39, 305, 369, 399
DriI GACNNNNNGTC 1 cut(s) 428
Eam1104I CTCTTC 2 cut(s) 148, 156
Eam1105I GACNNNNNGTC 1 cut(s) 428
EarI CTCTTC 2 cut(s) 148, 156
Eco24I GRGCYC 1 cut(s) 416
Eco57I CTGAAG 1 cut(s) 76
EcoRI GAATTC 1 cut(s) 542
EcoT38I GRGCYC 1 cut(s) 416
Esp3I CGTCTC 1 cut(s) 415
FaeI CATG 6 cut(s) 161, 272, 300, 327, 332, 396
FaqI GGGAC 1 cut(s) 411
FatI CATG 6 cut(s) 157, 268, 296, 323, 328, 392
FbaI TGATCA 1 cut(s) 39
Fnu4HI GCNGC 1 cut(s) 264
FokI GGATG 1 cut(s) 224
FriOI GRGCYC 1 cut(s) 416
Fsp4HI GCNGC 1 cut(s) 264
FspBI CTAG 2 cut(s) 12, 257
GlaI GCGC 1 cut(s) 313
GluI GCNGC 1 cut(s) 264
HhaI GCGC 1 cut(s) 314
Hin1I GRCGYC 1 cut(s) 424
Hin1II CATG 6 cut(s) 161, 272, 300, 327, 332, 396
Hin6I GCGC 1 cut(s) 312
HinP1I GCGC 1 cut(s) 312
HincII GTYRAC 1 cut(s) 205
HindII GTYRAC 1 cut(s) 205
HinfI GANTC 1 cut(s) 235
Hpy166II GTNNAC 2 cut(s) 98, 205
Hpy188I TCNGA 2 cut(s) 36, 126
Hpy188III TCNNGA 3 cut(s) 12, 158, 269
Hpy8I GTNNAC 2 cut(s) 98, 205
HpyAV CCTTC 2 cut(s) 284, 296
HpyCH4III ACNGT 1 cut(s) 107
HpyCH4IV ACGT 2 cut(s) 100, 424
HpyCH4V TGCA 3 cut(s) 74, 451, 493
HpyF3I CTNAG 1 cut(s) 33
HpySE526I ACGT 2 cut(s) 100, 424
Hsp92I GRCGYC 1 cut(s) 424
Hsp92II CATG 6 cut(s) 161, 272, 300, 327, 332, 396
HspAI GCGC 1 cut(s) 312
Ksp22I TGATCA 1 cut(s) 39
KspI CCGCGG 1 cut(s) 24
Kzo9I GATC 4 cut(s) 39, 305, 369, 399
LmnI GCTCC 2 cut(s) 64, 293
LpnPI CCDG 4 cut(s) 69, 382, 418, 482
Lsp1109I GCAGC 1 cut(s) 250
LweI GCATC 2 cut(s) 55, 139
MaeI CTAG 2 cut(s) 12, 257
MaeII ACGT 2 cut(s) 100, 424
MaeIII GTNAC 2 cut(s) 101, 193
MalI GATC 4 cut(s) 41, 307, 371, 401
MboI GATC 4 cut(s) 39, 305, 369, 399
MboII GAAGA 4 cut(s) 135, 173, 194, 379
MflI RGATCY 2 cut(s) 369, 399
MhlI GDGCHC 1 cut(s) 416
MluCI AATT 2 cut(s) 186, 542
MmeI TCCRAC 1 cut(s) 223
MnlI CCTC 2 cut(s) 157, 375
MseI TTAA 1 cut(s) 231
MslI CAYNNNNRTG 1 cut(s) 273
MspA1I CMGCKG 1 cut(s) 23
MvnI CGCG 1 cut(s) 23
NdeII GATC 4 cut(s) 39, 305, 369, 399
NlaIII CATG 6 cut(s) 161, 272, 300, 327, 332, 396
NmeAIII GCCGAG 1 cut(s) 512
NmuCI GTSAC 1 cut(s) 193
NspI RCATGY 3 cut(s) 300, 327, 396
PagI TCATGA 2 cut(s) 157, 268
PciI ACATGT 1 cut(s) 323
PfeI GAWTC 1 cut(s) 235
PkrI GCNGC 1 cut(s) 265
PscI ACATGT 1 cut(s) 323
PsuI RGATCY 2 cut(s) 369, 399
RseI CAYNNNNRTG 1 cut(s) 273
SacII CCGCGG 1 cut(s) 24
SaqAI TTAA 1 cut(s) 231
SatI GCNGC 1 cut(s) 264
Sau3AI GATC 4 cut(s) 39, 305, 369, 399
SduI GDGCHC 1 cut(s) 416
SetI ASST 5 cut(s) 61, 103, 265, 427, 520
SfaNI GCATC 2 cut(s) 55, 139
Sfr303I CCGCGG 1 cut(s) 24
SgrBI CCGCGG 1 cut(s) 24
SmiMI CAYNNNNRTG 1 cut(s) 273
Sse9I AATT 2 cut(s) 186, 542
SsiI CCGC 2 cut(s) 21, 23
SspMI CTAG 2 cut(s) 12, 257
TaaI ACNGT 1 cut(s) 107
TaiI ACGT 2 cut(s) 103, 427
TaqI TCGA 2 cut(s) 146, 365
TasI AATT 2 cut(s) 186, 542
TfiI GAWTC 1 cut(s) 235
Tru1I TTAA 1 cut(s) 231
Tru9I TTAA 1 cut(s) 231
TscAI CASTG 1 cut(s) 100
TseFI GTSAC 1 cut(s) 193
TseI GCWGC 1 cut(s) 263
Tsp45I GTSAC 1 cut(s) 193
TspDTI ATGAA 2 cut(s) 174, 285
TspGWI ACGGA 1 cut(s) 482
TspRI CASTG 1 cut(s) 100
XapI RAATTY 2 cut(s) 186, 542
XbaI TCTAGA 1 cut(s) 11
XceI RCATGY 3 cut(s) 300, 327, 396
XspI CTAG 2 cut(s) 12, 257
ZraI GACGTC 1 cut(s) 425
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.