pycom17g14360

DnaJ homolog subfamily B member

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0010346
Physical Location & Seq
Reverse (-)
11882497 .. 11886259
3763 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 645 bp
ATGGCTGATGGGGAAGAGAAAGGCAGTGACTTCTATGCAGTTTTAGGGTTGGACAAGGAATGCACAGACTCGGAGCTCAGAAATGCCTATAAGAAACTTGCACTGAGATGGCACCCGGATCGCTGTTCTGCCTCCGGAAATTCTAAGTTCGTGGAAGAAGCCAAAATGAAGTTCCAAGACATTCAACAAGCCTATTCTGTTTTATCTGACGCGAATAAGAGGTTTCTTTACGATGTAGGAGCCTATGGCAGTGATGATGACGAAAACAATGGGATGGGTGAGTTTTTGAACGAAATGGCAATGATGATGACCCAAACGAAGCCTAATGAAGATGGGAAGGAGACGTTTGAAGAATTGCAGGATCTTTTTGATGAAATGTTTCAAGGGGATATTGGGAGTTATGGGTCTGCTTCTCGGACCCCTAGTTCCTGTTCTACTTCTTCGTATGCGTCCTATTGTGAAAGCTCAAGTTCCAATAACAAGCGTAATTGCTCTGAAATGAACTATGAGAAGGTGACCTTGGATGATCCTTCTGTCTTCGACAATCATTTTCCGAGTTTTTGTGTAGGGACAGGTGGGAAGCCGTCAAGACAACGGGAAGGCAACAGCAGCAAGAGAAGGGATTCGCGCAGAAACCACCGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0005515 GO:0051087
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DnaJ

Protein Analysis

215

Amino Acids

24.09

Weight (kDa)

4.89

Isoelectric Point (pI)

59.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DnaJ PF00226 10 - 78 2.3e-20 DnaJ domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 111
AccII CGCG 2 cut(s) 212, 628
AccIII TCCGGA 1 cut(s) 134
AclWI GGATC 3 cut(s) 126, 369, 521
AcsI RAATTY 1 cut(s) 139
AgeI ACCGGT 1 cut(s) 639
AgsI TTSAA 4 cut(s) 185, 289, 350, 383
AjuI GAANNNNNNNTTGG 4 cut(s) 155, 187, 503, 535
AloI GAACNNNNNNTCC 2 cut(s) 409, 441
AluBI AGCT 2 cut(s) 76, 465
AluI AGCT 2 cut(s) 76, 465
Alw21I GWGCWC 1 cut(s) 78
Alw26I GTCTC 1 cut(s) 335
AlwI GGATC 3 cut(s) 126, 369, 521
Aor13HI TCCGGA 1 cut(s) 134
ApeKI GCWGC 1 cut(s) 609
ApoI RAATTY 1 cut(s) 139
AsiGI ACCGGT 1 cut(s) 639
Asp700I GAANNNNTTC 2 cut(s) 378, 622
AspLEI GCGC 1 cut(s) 630
AspS9I GGNCC 1 cut(s) 417
AsuC2I CCSGG 1 cut(s) 116
AsuHPI GGTGA 2 cut(s) 290, 526
AvaII GGWCC 1 cut(s) 417
BanI GGYRCC 1 cut(s) 111
BanII GRGCYC 1 cut(s) 78
BbsI GAAGAC 1 cut(s) 529
Bbv12I GWGCWC 1 cut(s) 78
BbvI GCAGC 1 cut(s) 621
BccI CCATC 4 cut(s) 2, 102, 268, 326
BceAI ACGGC 1 cut(s) 568
BcgI CGANNNNNNTGC 2 cut(s) 101, 135
BcnI CCSGG 1 cut(s) 116
BcoDI GTCTC 1 cut(s) 335
BfaI CTAG 1 cut(s) 423
BisI GCNGC 1 cut(s) 610
BlsI GCNGC 1 cut(s) 611
Bme1390I CCNGG 1 cut(s) 116
Bme18I GGWCC 1 cut(s) 417
BmgT120I GGNCC 1 cut(s) 417
BmiI GGNNCC 3 cut(s) 113, 241, 419
BmrFI CCNGG 1 cut(s) 116
BpiI GAAGAC 1 cut(s) 529
BpuEI CTTGAG 1 cut(s) 451
BpuMI CCSGG 1 cut(s) 116
BsaJI CCNNGG 1 cut(s) 519
BsaWI WCCGGW 2 cut(s) 134, 639
Bse118I RCCGGY 1 cut(s) 639
Bse3DI GCAATG 1 cut(s) 306
BseAI TCCGGA 1 cut(s) 134
BseDI CCNNGG 1 cut(s) 519
BseGI GGATG 2 cut(s) 279, 529
BseMI GCAATG 1 cut(s) 306
BseMII CTCAG 2 cut(s) 91, 95
BseXI GCAGC 1 cut(s) 621
Bsh1236I CGCG 2 cut(s) 212, 628
BshNI GGYRCC 1 cut(s) 111
BshTI ACCGGT 1 cut(s) 639
BsiHKAI GWGCWC 1 cut(s) 78
BsiSI CCGG 3 cut(s) 116, 135, 640
BslFI GGGAC 1 cut(s) 583
BsmAI GTCTC 1 cut(s) 335
BsmBI CGTCTC 1 cut(s) 335
BsmFI GGGAC 1 cut(s) 583
BsmI GAATGC 1 cut(s) 65
Bsp1286I GDGCHC 1 cut(s) 78
Bsp13I TCCGGA 1 cut(s) 134
Bsp143I GATC 3 cut(s) 118, 361, 526
BspCNI CTCAG 2 cut(s) 90, 96
BspEI TCCGGA 1 cut(s) 134
BspFNI CGCG 2 cut(s) 212, 628
BspLI GGNNCC 3 cut(s) 113, 241, 419
BspPI GGATC 3 cut(s) 126, 369, 521
BspT107I GGYRCC 1 cut(s) 111
BsrDI GCAATG 1 cut(s) 306
BsrFI RCCGGY 1 cut(s) 639
BssAI RCCGGY 1 cut(s) 639
BssECI CCNNGG 1 cut(s) 519
BssMI GATC 3 cut(s) 118, 361, 526
BssT1I CCWWGG 1 cut(s) 519
Bst6I CTCTTC 1 cut(s) 9
BstDEI CTNAG 3 cut(s) 77, 104, 144
BstEII GGTNACC 1 cut(s) 514
BstF5I GGATG 2 cut(s) 279, 529
BstFNI CGCG 2 cut(s) 212, 628
BstHHI GCGC 1 cut(s) 630
BstKTI GATC 3 cut(s) 121, 364, 529
BstMAI GTCTC 1 cut(s) 335
BstMBI GATC 3 cut(s) 118, 361, 526
BstMWI GCNNNNNNNGC 1 cut(s) 609
BstPI GGTNACC 1 cut(s) 514
BstSCI CCNGG 1 cut(s) 114
BstUI CGCG 2 cut(s) 212, 628
BstV1I GCAGC 1 cut(s) 621
BstV2I GAAGAC 1 cut(s) 529
BstX2I RGATCY 1 cut(s) 361
BstYI RGATCY 1 cut(s) 361
BtsCI GGATG 2 cut(s) 279, 529
BtsI GCAGTG 2 cut(s) 31, 256
BtsIMutI CAGTG 3 cut(s) 31, 101, 256
CfoI GCGC 1 cut(s) 630
Cfr10I RCCGGY 1 cut(s) 639
Cfr13I GGNCC 1 cut(s) 417
CseI GACGC 2 cut(s) 218, 438
CspAI ACCGGT 1 cut(s) 639
CviJI RGCY 8 cut(s) 5, 76, 161, 191, 242, 322, 465, 583
CviKI_1 RGCY 8 cut(s) 5, 76, 161, 191, 242, 322, 465, 583
DdeI CTNAG 3 cut(s) 77, 104, 144
DpnI GATC 3 cut(s) 120, 363, 528
DpnII GATC 3 cut(s) 118, 361, 526
Eam1104I CTCTTC 1 cut(s) 9
EarI CTCTTC 1 cut(s) 9
Ecl136II GAGCTC 1 cut(s) 76
Eco130I CCWWGG 1 cut(s) 519
Eco24I GRGCYC 1 cut(s) 78
Eco47I GGWCC 1 cut(s) 417
Eco53kI GAGCTC 1 cut(s) 76
Eco91I GGTNACC 1 cut(s) 514
EcoICRI GAGCTC 1 cut(s) 76
EcoO65I GGTNACC 1 cut(s) 514
EcoT14I CCWWGG 1 cut(s) 519
EcoT38I GRGCYC 1 cut(s) 78
ErhI CCWWGG 1 cut(s) 519
Esp3I CGTCTC 1 cut(s) 335
FaiI YATR 6 cut(s) 36, 90, 246, 402, 447, 507
FalI AAGNNNNNCTT 2 cut(s) 503, 535
FaqI GGGAC 1 cut(s) 583
Fnu4HI GCNGC 1 cut(s) 610
FokI GGATG 2 cut(s) 286, 536
FriOI GRGCYC 1 cut(s) 78
Fsp4HI GCNGC 1 cut(s) 610
FspBI CTAG 1 cut(s) 423
GlaI GCGC 1 cut(s) 629
GluI GCNGC 1 cut(s) 610
HapII CCGG 3 cut(s) 116, 135, 640
HgaI GACGC 2 cut(s) 218, 438
HhaI GCGC 1 cut(s) 630
Hin6I GCGC 1 cut(s) 628
HinP1I GCGC 1 cut(s) 628
HinfI GANTC 2 cut(s) 68, 623
HpaII CCGG 3 cut(s) 116, 135, 640
HphI GGTGA 2 cut(s) 290, 526
Hpy188I TCNGA 6 cut(s) 73, 80, 208, 417, 496, 555
Hpy188III TCNNGA 2 cut(s) 135, 588
HpyAV CCTTC 5 cut(s) 331, 505, 540, 593, 612
HpyCH4IV ACGT 1 cut(s) 344
HpyCH4V TGCA 4 cut(s) 38, 63, 101, 358
HpyF10VI GCNNNNNNNGC 1 cut(s) 609
HpyF3I CTNAG 3 cut(s) 77, 104, 144
HpySE526I ACGT 1 cut(s) 344
HspAI GCGC 1 cut(s) 628
Kpn2I TCCGGA 1 cut(s) 134
Kzo9I GATC 3 cut(s) 118, 361, 526
LmnI GCTCC 2 cut(s) 73, 239
LpnPI CCDG 5 cut(s) 129, 148, 344, 442, 558
Lsp1109I GCAGC 1 cut(s) 621
MaeI CTAG 1 cut(s) 423
MaeII ACGT 1 cut(s) 344
MaeIII GTNAC 2 cut(s) 26, 514
MalI GATC 3 cut(s) 120, 363, 528
MboI GATC 3 cut(s) 118, 361, 526
MboII GAAGA 6 cut(s) 26, 167, 341, 362, 432, 529
MflI RGATCY 1 cut(s) 361
MhlI GDGCHC 1 cut(s) 78
MluCI AATT 3 cut(s) 139, 353, 487
MlyI GAGTC 1 cut(s) 62
MmeI TCCRAC 1 cut(s) 30
MnlI CCTC 2 cut(s) 142, 213
MroI TCCGGA 1 cut(s) 134
MroXI GAANNNNTTC 2 cut(s) 378, 622
MslI CAYNNNNRTG 1 cut(s) 106
MspI CCGG 3 cut(s) 116, 135, 640
MspR9I CCNGG 1 cut(s) 116
Mva1269I GAATGC 1 cut(s) 65
MvnI CGCG 2 cut(s) 212, 628
MwoI GCNNNNNNNGC 1 cut(s) 609
NciI CCSGG 1 cut(s) 116
NdeII GATC 3 cut(s) 118, 361, 526
NlaIV GGNNCC 3 cut(s) 113, 241, 419
NmuCI GTSAC 2 cut(s) 26, 514
PctI GAATGC 1 cut(s) 65
PdmI GAANNNNTTC 2 cut(s) 378, 622
PfeI GAWTC 1 cut(s) 623
PinAI ACCGGT 1 cut(s) 639
PkrI GCNGC 1 cut(s) 611
PleI GAGTC 1 cut(s) 62
PpsI GAGTC 1 cut(s) 62
Psp124BI GAGCTC 1 cut(s) 78
PspEI GGTNACC 1 cut(s) 514
PspN4I GGNNCC 3 cut(s) 113, 241, 419
PspPI GGNCC 1 cut(s) 417
PsuI RGATCY 1 cut(s) 361
RseI CAYNNNNRTG 1 cut(s) 106
SacI GAGCTC 1 cut(s) 78
SatI GCNGC 1 cut(s) 610
Sau3AI GATC 3 cut(s) 118, 361, 526
Sau96I GGNCC 1 cut(s) 417
SchI GAGTC 1 cut(s) 62
ScrFI CCNGG 1 cut(s) 116
SduI GDGCHC 1 cut(s) 78
SetI ASST 7 cut(s) 78, 224, 347, 467, 516, 521, 577
SinI GGWCC 1 cut(s) 417
SmiMI CAYNNNNRTG 1 cut(s) 106
SmlI CTYRAG 1 cut(s) 466
SmoI CTYRAG 1 cut(s) 466
Sse9I AATT 3 cut(s) 139, 353, 487
SspMI CTAG 1 cut(s) 423
SstI GAGCTC 1 cut(s) 78
StyD4I CCNGG 1 cut(s) 114
StyI CCWWGG 1 cut(s) 519
TaiI ACGT 1 cut(s) 347
TaqI TCGA 1 cut(s) 540
TasI AATT 3 cut(s) 139, 353, 487
TfiI GAWTC 1 cut(s) 623
TscAI CASTG 3 cut(s) 31, 108, 256
TseFI GTSAC 2 cut(s) 26, 514
TseI GCWGC 1 cut(s) 609
Tsp45I GTSAC 2 cut(s) 26, 514
TspDTI ATGAA 4 cut(s) 182, 342, 387, 515
TspRI CASTG 3 cut(s) 31, 108, 256
VpaK11BI GGWCC 1 cut(s) 417
XapI RAATTY 1 cut(s) 139
XmnI GAANNNNTTC 2 cut(s) 378, 622
XspI CTAG 1 cut(s) 423
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.