pycom17g15550

Protein of unknown function (DUF3754)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0005827
Physical Location & Seq
Forward (+)
13580989 .. 13587047
6059 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 2265 bp
ATGGCTTGGGTACTGTTATGTATTTTCCTTATCGTATATACGTGGGGTTGGCAGTGGCACACTGCTCTGCTTTGCTGCTCTCTCGACCTCCTTTGCTTTGCGATCATGTCAGCTTCTTGGCACGCTCTCACCGGCCTCGGAGCTCTCTCCGTAACCGCAAAACAGCGCCGTCTAATTCCTTCACCACCATCAGTTGCTCACTGCTTTCTCGCCAGCTTCCAAGAACTGTCGACGGAGCCTCCGCGAACCCAAACCACCGGCGCCGCTATAAGCTTGAAAGACGCCGGCAAGACCGAGAAGCAAAACGACGTCGTTCTCGATCATAACCAAGCCGAAGAAGAAGAAGAAGACCATGGAATTTCCAAAATCCCGGTCCCCAGGCAGAAGTACATCCCCGTTTCCAAGGCCGAGCTTCTCGACGGCATCGTTTCGAACTTGTTCAAGGACAGCAACGGTGAAGACGACGATGAATTGCAGAGCTTTCTTCTTCTCTCTTCGTGCTTGGACTCCATTCTTCACGCCGAGCACAAGGGAATTTTGGAAGAAATGCGAGCTGATTATTTCAAATCGAATTCCGCGGAGAACAAGCAAAATGGTGATTTGGTTGATGGGCAGAGCTCGGATTCTGCTAATGGGAGTGTCATCAATGGAGTTGAGAGCATTGAGGCAAATGAGGATGAAAAAAATGAAGGTGGAAACGCAATGCCGCAGTTAGATTATGTTTTGGAGTTGCGAAAACTCTTGGGTTCTCCGGCTAAGAACGTTGAGAGAGTTGCTGTTTCCACTCGTTTCCAGCGTTCTTTCATGCAACTTCTTAATGATGCTCAGTTTGAAGAACTATCCGAAGGGGATTTAATGCTGACTTCTGCATTGAACACAGATTATCTCCTTACTTTGCCCATATATGTTGACTGGAAGAAGGCATCTGAGTCCAATGCAATTATATTCAGGCGAGGATATGCAACAGAGAGGGAGAAGGGTCTACTACTTGTAGAAAAACTGGAGTACATACAGTCCAAAATTCTACAAGGAACCTTCTTCATCGTATCAAAACCGTTGGCGAAATTTGGGAGCTGGATAGTTGAGGCATTCGAAGTTGCTTGCGAAACACCAGAAGTACAAGACTTGACTAAAAGAATGAAGCTTTGGCTTAAGGAATTGTCTGTTTTCCAGCAAGCATTTCGTTATAGCGAACAAACTTTTGATGATCTGCTGGGAGTAGAGGAACTATCAAATAAAGACCTCCCAATTTGGCTGGCGGCACAGAGGGCAGTATCTCGTTATGAAGGGCGTTTATCTTCAGTTGGACCCCGTGGAAGGCTCCTAAAGAAGTTGCTTTCATGGATTGGACTTATTTCCCCTACACCAGAAACACCATTTGAGCTGGATGGCGATACTAATGATTCTGACCCTTATGCAAGGCCGATCTTCTTATCAAGGATATCACTTAGTGACATATGGAGGCCTGCTACTAGGAAATACTGTGGAAATGATATTTGGAAAATGCTAAAAACTTCTGTTTCTATTCTCTTCTCACAGTCCATCCTCCAGGAGGCAGCATTTCAAGAATTAATTTTGCTTTATACCCAAGAAGTAGATGGCAGGAATGCTGACTACAATGCTACTGTTCCATCGTTGCAGTTAAAGATTTATGAGAAAATTCCCATTCCGGATCTACCAGTTATCTTTCCCCACAAAAAGCTGTCTTTCCGTATCATAGACACGGTGCGCTTGGACATTGCCTCAATACTGGGGCTTTCGGCATTCTTCATAAACTACAAATTTTTTAATGTCCTATCTTCCCCGTCAGCAATAGTTCTTGATGTGGTTGCAATCAGCGCTCTTATAATTTATGTGTCCCGTGTGGTTTTGGGATACAAACAGACGTGGGATAGGTATCAACTTCTAGTCAACACGACGCTTTATGAGAAAACCTTGGCAAGTGGATTTGGCTCAGTTCATTTTCTTCTGGATGCTTCTGAACAGCAACAATACAAGGAGGCCATTTTGGCGTATGCAATCCTTCTCAAAGCAAACAAGGGGAAGCTCACGTGTAGCCAGAGTGTTGGAGACAACTGCGAAAGATTTATGTATGATGTGTTTAAACTAAAGGTTGAAATGCCAGTTGACAAGGCAGTTGCTACATTATTAAGGCTGGGGTTAGCTACAGAAACTCCTATTGACGGAAGGATTAGTTTGGAGGCCATTTCCTGTTCAAAGGCACACAATTCCCTGAAAGAACGTTGGAATAGTTTGCTCGGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3754

Protein Analysis

755

Amino Acids

84.62

Weight (kDa)

5.26

Isoelectric Point (pI)

41.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF3754 PF12576 521 - 651 3.6e-32 Protein of unknown function (DUF3754)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1847
AatII GACGTC 1 cut(s) 312
AccB1I GGYRCC 1 cut(s) 260
AccI GTMKAC 2 cut(s) 230, 982
AccII CGCG 2 cut(s) 244, 578
AccIII TCCGGA 1 cut(s) 1669
AciI CCGC 7 cut(s) 156, 242, 264, 576, 578, 707, 1259
AclI AACGTT 2 cut(s) 762, 2242
AclWI GGATC 1 cut(s) 1680
AcsI RAATTY 7 cut(s) 357, 534, 571, 1020, 1064, 1659, 1781
AcuI CTGAAG 1 cut(s) 1284
AcvI CACGTG 1 cut(s) 2052
AcyI GRCGYC 3 cut(s) 261, 282, 309
AdeI CACNNNGTG 1 cut(s) 1451
AfaI GTAC 4 cut(s) 12, 389, 1007, 1119
AfeI AGCGCT 1 cut(s) 1840
AfiI CCNNNNNNNGG 3 cut(s) 1317, 1552, 2183
AflII CTTAAG 1 cut(s) 1151
AflIII ACRYGT 1 cut(s) 2051
AgsI TTSAA 8 cut(s) 277, 442, 565, 833, 874, 1565, 2117, 2217
AjiI CACGTC 1 cut(s) 1887
AjnI CCWGG 2 cut(s) 377, 1548
AjuI GAANNNNNNNTTGG 4 cut(s) 1129, 1161, 1480, 1512
Alw21I GWGCWC 3 cut(s) 145, 528, 620
Alw26I GTCTC 1 cut(s) 2064
AlwI GGATC 1 cut(s) 1680
Aor13HI TCCGGA 1 cut(s) 1669
Aor51HI AGCGCT 1 cut(s) 1840
AoxI GGCC 6 cut(s) 133, 405, 1421, 1463, 2001, 2202
ApeKI GCWGC 2 cut(s) 75, 1556
ApoI RAATTY 7 cut(s) 357, 534, 571, 1020, 1064, 1659, 1781
ArsI GACNNNNNNTTYG 2 cut(s) 357, 389
AseI ATTAAT 1 cut(s) 1571
Asp700I GAANNNNTTC 1 cut(s) 437
AspLEI GCGC 4 cut(s) 168, 263, 1731, 1841
AspS9I GGNCC 2 cut(s) 373, 1307
AsuC2I CCSGG 1 cut(s) 371
AsuHPI GGTGA 4 cut(s) 121, 174, 467, 608
AsuII TTCGAA 2 cut(s) 431, 1092
AvaII GGWCC 2 cut(s) 373, 1307
BanI GGYRCC 1 cut(s) 260
BanII GRGCYC 2 cut(s) 145, 620
BbrPI CACGTG 1 cut(s) 2052
BbsI GAAGAC 2 cut(s) 354, 465
Bbv12I GWGCWC 3 cut(s) 145, 528, 620
BbvI GCAGC 2 cut(s) 62, 1568
BccI CCATC 6 cut(s) 196, 602, 1382, 1550, 1592, 1639
BceAI ACGGC 2 cut(s) 153, 436
BcgI CGANNNNNNTGC 2 cut(s) 1163, 1197
BciT130I CCWGG 2 cut(s) 379, 1550
BciVI GTATCC 1 cut(s) 1868
BcnI CCSGG 1 cut(s) 371
BcoDI GTCTC 1 cut(s) 2064
BfaI CTAG 2 cut(s) 1473, 1907
BfmI CTRYAG 1 cut(s) 2166
BfoI RGCGCY 3 cut(s) 169, 264, 1842
BfrI CTTAAG 1 cut(s) 1151
BfuI GTATCC 1 cut(s) 1868
BglI GCCNNNNNGGC 1 cut(s) 2009
BisI GCNGC 5 cut(s) 76, 264, 707, 1260, 1557
BlsI GCNGC 5 cut(s) 77, 265, 708, 1261, 1558
Bme1390I CCNGG 3 cut(s) 371, 379, 1550
Bme18I GGWCC 2 cut(s) 373, 1307
BmgBI CACGTC 1 cut(s) 1887
BmgT120I GGNCC 2 cut(s) 373, 1307
BmiI GGNNCC 6 cut(s) 237, 262, 375, 1033, 1309, 1322
BmrFI CCNGG 3 cut(s) 371, 379, 1550
BmrI ACTGGG 1 cut(s) 1760
BmsI GCATC 4 cut(s) 432, 811, 932, 1963
BmuI ACTGGG 1 cut(s) 1760
BpiI GAAGAC 2 cut(s) 354, 465
BpmI CTGGAG 2 cut(s) 1022, 1532
Bpu14I TTCGAA 2 cut(s) 431, 1092
BpuMI CCSGG 1 cut(s) 371
BsaAI YACGTR 2 cut(s) 42, 2052
BsaBI GATNNNNATC 1 cut(s) 1896
BsaHI GRCGYC 3 cut(s) 261, 282, 309
BsaJI CCNNGG 7 cut(s) 136, 352, 377, 402, 576, 1312, 1935
BsaWI WCCGGW 1 cut(s) 1669
BsaXI ACNNNNNCTCC 4 cut(s) 223, 253, 2158, 2188
Bsc4I CCNNNNNNNGG 3 cut(s) 1317, 1552, 2183
Bse118I RCCGGY 3 cut(s) 131, 257, 284
Bse1I ACTGG 5 cut(s) 917, 1005, 1679, 1755, 2123
Bse3DI GCAATG 2 cut(s) 708, 1737
Bse8I GATNNNNATC 1 cut(s) 1896
BseAI TCCGGA 1 cut(s) 1669
BseBI CCWGG 2 cut(s) 379, 1550
BseDI CCNNGG 7 cut(s) 136, 352, 377, 402, 576, 1312, 1935
BseGI GGATG 5 cut(s) 390, 682, 1393, 1542, 1978
BseJI GATNNNNATC 1 cut(s) 1896
BseLI CCNNNNNNNGG 3 cut(s) 1317, 1552, 2183
BseMI GCAATG 2 cut(s) 708, 1737
BseMII CTCAG 3 cut(s) 839, 918, 1968
BseNI ACTGG 5 cut(s) 917, 1005, 1679, 1755, 2123
BseXI GCAGC 2 cut(s) 62, 1568
BseYI CCCAGC 2 cut(s) 1213, 2155
Bsh1236I CGCG 2 cut(s) 244, 578
BshFI GGCC 6 cut(s) 135, 407, 1423, 1465, 2003, 2204
BshNI GGYRCC 1 cut(s) 260
BsiHKAI GWGCWC 3 cut(s) 145, 528, 620
BsiSI CCGG 6 cut(s) 132, 258, 285, 371, 752, 1670
BslFI GGGAC 2 cut(s) 359, 1843
BslI CCNNNNNNNGG 3 cut(s) 1317, 1552, 2183
BsmAI GTCTC 1 cut(s) 2064
BsmFI GGGAC 2 cut(s) 359, 1843
BsmI GAATGC 3 cut(s) 1088, 1612, 1763
BsnI GGCC 6 cut(s) 135, 407, 1423, 1465, 2003, 2204
Bsp119I TTCGAA 2 cut(s) 431, 1092
Bsp1286I GDGCHC 3 cut(s) 145, 528, 620
Bsp13I TCCGGA 1 cut(s) 1669
Bsp143I GATC 5 cut(s) 102, 319, 1207, 1425, 1672
Bsp19I CCATGG 1 cut(s) 352
BspACI CCGC 7 cut(s) 156, 242, 264, 576, 578, 707, 1259
BspANI GGCC 6 cut(s) 135, 407, 1423, 1465, 2003, 2204
BspCNI CTCAG 3 cut(s) 838, 919, 1967
BspEI TCCGGA 1 cut(s) 1669
BspFNI CGCG 2 cut(s) 244, 578
BspLI GGNNCC 6 cut(s) 237, 262, 375, 1033, 1309, 1322
BspPI GGATC 1 cut(s) 1680
BspT104I TTCGAA 2 cut(s) 431, 1092
BspT107I GGYRCC 1 cut(s) 260
BspTI CTTAAG 1 cut(s) 1151
BsrDI GCAATG 2 cut(s) 708, 1737
BsrFI RCCGGY 3 cut(s) 131, 257, 284
BsrI ACTGG 5 cut(s) 917, 1005, 1679, 1755, 2123
BssAI RCCGGY 3 cut(s) 131, 257, 284
BssECI CCNNGG 7 cut(s) 136, 352, 377, 402, 576, 1312, 1935
BssMI GATC 5 cut(s) 102, 319, 1207, 1425, 1672
BssNI GRCGYC 3 cut(s) 261, 282, 309
BssT1I CCWWGG 3 cut(s) 352, 402, 1935
Bst2UI CCWGG 2 cut(s) 379, 1550
Bst4CI ACNGT 9 cut(s) 15, 228, 455, 1014, 1056, 1484, 1539, 1627, 1726
Bst6I CTCTTC 2 cut(s) 499, 1535
BstACI GRCGYC 3 cut(s) 261, 282, 309
BstAFI CTTAAG 1 cut(s) 1151
BstBAI YACGTR 2 cut(s) 42, 2052
BstBI TTCGAA 2 cut(s) 431, 1092
BstC8I GCNNGC 8 cut(s) 123, 214, 286, 552, 1102, 1176, 1257, 1467
BstDEI CTNAG 5 cut(s) 756, 825, 927, 1448, 1954
BstDSI CCRYGG 3 cut(s) 352, 576, 1312
BstENI CCTNNNNNAGG 1 cut(s) 1550
BstF5I GGATG 5 cut(s) 390, 682, 1393, 1542, 1978
BstFNI CGCG 2 cut(s) 244, 578
BstH2I RGCGCY 3 cut(s) 169, 264, 1842
BstHHI GCGC 4 cut(s) 168, 263, 1731, 1841
BstKTI GATC 5 cut(s) 105, 322, 1210, 1428, 1675
BstMAI GTCTC 1 cut(s) 2064
BstMBI GATC 5 cut(s) 102, 319, 1207, 1425, 1672
BstMWI GCNNNNNNNGC 3 cut(s) 1268, 1838, 2009
BstNI CCWGG 2 cut(s) 379, 1550
BstSCI CCNGG 3 cut(s) 369, 377, 1548
BstSFI CTRYAG 1 cut(s) 2166
BstUI CGCG 2 cut(s) 244, 578
BstV1I GCAGC 2 cut(s) 62, 1568
BstV2I GAAGAC 2 cut(s) 354, 465
BstX2I RGATCY 1 cut(s) 1672
BstXI CCANNNNNNTGG 1 cut(s) 2066
BstYI RGATCY 1 cut(s) 1672
BsuI GTATCC 1 cut(s) 1868
BsuRI GGCC 6 cut(s) 135, 407, 1423, 1465, 2003, 2204
BtgI CCRYGG 3 cut(s) 352, 576, 1312
BtrI CACGTC 1 cut(s) 1887
BtsCI GGATG 5 cut(s) 390, 682, 1393, 1542, 1978
BtsI GCAGTG 3 cut(s) 59, 60, 199
BtsIMutI CAGTG 3 cut(s) 59, 60, 199
Cac8I GCNNGC 8 cut(s) 123, 214, 286, 552, 1102, 1176, 1257, 1467
CfoI GCGC 4 cut(s) 168, 263, 1731, 1841
Cfr10I RCCGGY 3 cut(s) 131, 257, 284
Cfr13I GGNCC 2 cut(s) 373, 1307
Cfr42I CCGCGG 1 cut(s) 579
CseI GACGC 2 cut(s) 290, 1927
Csp6I GTAC 4 cut(s) 11, 388, 1006, 1118
CviAII CATG 4 cut(s) 106, 353, 805, 1341
CviQI GTAC 4 cut(s) 11, 388, 1006, 1118
DdeI CTNAG 5 cut(s) 756, 825, 927, 1448, 1954
DinI GGCGCC 1 cut(s) 262
DpnI GATC 5 cut(s) 104, 321, 1209, 1427, 1674
DpnII GATC 5 cut(s) 102, 319, 1207, 1425, 1672
DraI TTTAAA 1 cut(s) 2104
DraIII CACNNNGTG 1 cut(s) 1451
Eam1104I CTCTTC 2 cut(s) 499, 1535
EarI CTCTTC 2 cut(s) 499, 1535
Ecl136II GAGCTC 2 cut(s) 143, 618
Eco130I CCWWGG 3 cut(s) 352, 402, 1935
Eco147I AGGCCT 1 cut(s) 1465
Eco24I GRGCYC 2 cut(s) 145, 620
Eco32I GATATC 1 cut(s) 1443
Eco47I GGWCC 2 cut(s) 373, 1307
Eco47III AGCGCT 1 cut(s) 1840
Eco53kI GAGCTC 2 cut(s) 143, 618
Eco57I CTGAAG 1 cut(s) 1284
Eco72I CACGTG 1 cut(s) 2052
EcoICRI GAGCTC 2 cut(s) 143, 618
EcoNI CCTNNNNNAGG 1 cut(s) 1550
EcoRI GAATTC 1 cut(s) 571
EcoRII CCWGG 2 cut(s) 377, 1548
EcoRV GATATC 1 cut(s) 1443
EcoT14I CCWWGG 3 cut(s) 352, 402, 1935
EcoT38I GRGCYC 2 cut(s) 145, 620
EgeI GGCGCC 1 cut(s) 262
EheI GGCGCC 1 cut(s) 262
ErhI CCWWGG 3 cut(s) 352, 402, 1935
FaeI CATG 4 cut(s) 109, 356, 808, 1344
FaqI GGGAC 2 cut(s) 359, 1843
FatI CATG 4 cut(s) 105, 352, 804, 1340
FauNDI CATATG 1 cut(s) 1457
FblI GTMKAC 2 cut(s) 230, 982
Fnu4HI GCNGC 5 cut(s) 76, 264, 707, 1260, 1557
FokI GGATG 5 cut(s) 377, 689, 1400, 1529, 1985
FriOI GRGCYC 2 cut(s) 145, 620
Fsp4HI GCNGC 5 cut(s) 76, 264, 707, 1260, 1557
FspBI CTAG 2 cut(s) 1473, 1907
GlaI GCGC 4 cut(s) 167, 262, 1730, 1840
GluI GCNGC 5 cut(s) 76, 264, 707, 1260, 1557
GsaI CCCAGC 2 cut(s) 1217, 2159
GsuI CTGGAG 2 cut(s) 1022, 1532
HaeII RGCGCY 3 cut(s) 169, 264, 1842
HaeIII GGCC 6 cut(s) 135, 407, 1423, 1465, 2003, 2204
HapII CCGG 6 cut(s) 132, 258, 285, 371, 752, 1670
HgaI GACGC 2 cut(s) 290, 1927
HhaI GCGC 4 cut(s) 168, 263, 1731, 1841
Hin1I GRCGYC 3 cut(s) 261, 282, 309
Hin1II CATG 4 cut(s) 109, 356, 808, 1344
Hin6I GCGC 4 cut(s) 166, 261, 1729, 1839
HinP1I GCGC 4 cut(s) 166, 261, 1729, 1839
HincII GTYRAC 4 cut(s) 231, 910, 1912, 2128
HindII GTYRAC 4 cut(s) 231, 910, 1912, 2128
HindIII AAGCTT 2 cut(s) 271, 1142
HinfI GANTC 4 cut(s) 506, 623, 929, 1403
HpaII CCGG 6 cut(s) 132, 258, 285, 371, 752, 1670
HphI GGTGA 4 cut(s) 121, 174, 467, 608
Hpy166II GTNNAC 5 cut(s) 231, 910, 983, 1912, 2128
Hpy188I TCNGA 6 cut(s) 140, 622, 844, 928, 1408, 1981
Hpy188III TCNNGA 7 cut(s) 83, 317, 416, 1565, 1670, 1820, 1970
Hpy8I GTNNAC 5 cut(s) 231, 910, 983, 1912, 2128
Hpy99I CGWCG 6 cut(s) 235, 311, 314, 422, 467, 1921
HpyCH4III ACNGT 9 cut(s) 15, 228, 455, 1014, 1056, 1484, 1539, 1627, 1726
HpyCH4IV ACGT 6 cut(s) 41, 309, 762, 1886, 2051, 2242
HpyCH4V TGCA 9 cut(s) 475, 808, 869, 938, 962, 1418, 1639, 1832, 2018
HpyF10VI GCNNNNNNNGC 3 cut(s) 1268, 1838, 2009
HpyF3I CTNAG 5 cut(s) 756, 825, 927, 1448, 1954
HpySE526I ACGT 6 cut(s) 41, 309, 762, 1886, 2051, 2242
Hsp92I GRCGYC 3 cut(s) 261, 282, 309
Hsp92II CATG 4 cut(s) 109, 356, 808, 1344
HspAI GCGC 4 cut(s) 166, 261, 1729, 1839
KasI GGCGCC 1 cut(s) 260
Kpn2I TCCGGA 1 cut(s) 1669
KroI GCCGGC 1 cut(s) 284
KroNI GCCGGC 1 cut(s) 286
KspI CCGCGG 1 cut(s) 579
Kzo9I GATC 5 cut(s) 102, 319, 1207, 1425, 1672
LmnI GCTCC 4 cut(s) 140, 235, 1071, 1326
Lsp1109I GCAGC 2 cut(s) 62, 1568
LweI GCATC 4 cut(s) 432, 811, 932, 1963
MaeI CTAG 2 cut(s) 1473, 1907
MaeII ACGT 6 cut(s) 41, 309, 762, 1886, 2051, 2242
MaeIII GTNAC 2 cut(s) 151, 1451
MalI GATC 5 cut(s) 104, 321, 1209, 1427, 1674
MboI GATC 5 cut(s) 102, 319, 1207, 1425, 1672
MflI RGATCY 1 cut(s) 1672
MhlI GDGCHC 3 cut(s) 145, 528, 620
Mly113I GGCGCC 1 cut(s) 261
MlyI GAGTC 2 cut(s) 500, 938
MmeI TCCRAC 3 cut(s) 1285, 2047, 2225
MroI TCCGGA 1 cut(s) 1669
MroNI GCCGGC 1 cut(s) 284
MroXI GAANNNNTTC 1 cut(s) 437
MseI TTAA 9 cut(s) 816, 854, 1152, 1571, 1643, 1788, 2103, 2150, 2263
MspA1I CMGCKG 1 cut(s) 578
MspCI CTTAAG 1 cut(s) 1151
MspI CCGG 6 cut(s) 132, 258, 285, 371, 752, 1670
MspR9I CCNGG 3 cut(s) 371, 379, 1550
MssI GTTTAAAC 1 cut(s) 2104
Mva1269I GAATGC 3 cut(s) 1088, 1612, 1763
MvaI CCWGG 2 cut(s) 379, 1550
MvnI CGCG 2 cut(s) 244, 578
MwoI GCNNNNNNNGC 3 cut(s) 1268, 1838, 2009
NaeI GCCGGC 1 cut(s) 286
NarI GGCGCC 1 cut(s) 261
NciI CCSGG 1 cut(s) 371
NcoI CCATGG 1 cut(s) 352
NdeI CATATG 1 cut(s) 1457
NdeII GATC 5 cut(s) 102, 319, 1207, 1425, 1672
NgoMIV GCCGGC 1 cut(s) 284
NlaIII CATG 4 cut(s) 109, 356, 808, 1344
NlaIV GGNNCC 6 cut(s) 237, 262, 375, 1033, 1309, 1322
NmeAIII GCCGAG 2 cut(s) 433, 547
NmuCI GTSAC 1 cut(s) 1451
NspV TTCGAA 2 cut(s) 431, 1092
PceI AGGCCT 1 cut(s) 1465
PcsI WCGNNNNNNNCGW 4 cut(s) 315, 423, 459, 793
PctI GAATGC 3 cut(s) 1088, 1612, 1763
PdiI GCCGGC 1 cut(s) 286
PdmI GAANNNNTTC 1 cut(s) 437
PfeI GAWTC 2 cut(s) 623, 1403
PfoI TCCNGGA 1 cut(s) 1548
PkrI GCNGC 5 cut(s) 77, 265, 708, 1261, 1558
PleI GAGTC 2 cut(s) 500, 937
PluTI GGCGCC 1 cut(s) 264
PmaCI CACGTG 1 cut(s) 2052
PmeI GTTTAAAC 1 cut(s) 2104
PmlI CACGTG 1 cut(s) 2052
PpsI GAGTC 2 cut(s) 500, 937
Ppu21I YACGTR 2 cut(s) 42, 2052
PshBI ATTAAT 1 cut(s) 1571
PsiI TTATAA 1 cut(s) 1847
Psp124BI GAGCTC 2 cut(s) 145, 620
Psp1406I AACGTT 2 cut(s) 762, 2242
Psp6I CCWGG 2 cut(s) 377, 1548
PspCI CACGTG 1 cut(s) 2052
PspFI CCCAGC 2 cut(s) 1213, 2155
PspGI CCWGG 2 cut(s) 377, 1548
PspN4I GGNNCC 6 cut(s) 237, 262, 375, 1033, 1309, 1322
PspPI GGNCC 2 cut(s) 373, 1307
PsuI RGATCY 1 cut(s) 1672
RsaI GTAC 4 cut(s) 12, 389, 1007, 1119
RsaNI GTAC 4 cut(s) 11, 388, 1006, 1118
SacI GAGCTC 2 cut(s) 145, 620
SacII CCGCGG 1 cut(s) 579
SalI GTCGAC 1 cut(s) 229
SaqAI TTAA 9 cut(s) 816, 854, 1152, 1571, 1643, 1788, 2103, 2150, 2263
SatI GCNGC 5 cut(s) 76, 264, 707, 1260, 1557
Sau3AI GATC 5 cut(s) 102, 319, 1207, 1425, 1672
Sau96I GGNCC 2 cut(s) 373, 1307
SchI GAGTC 2 cut(s) 500, 938
ScrFI CCNGG 3 cut(s) 371, 379, 1550
SduI GDGCHC 3 cut(s) 145, 528, 620
SfaNI GCATC 4 cut(s) 432, 811, 932, 1963
SfcI CTRYAG 1 cut(s) 2166
SfoI GGCGCC 1 cut(s) 262
Sfr303I CCGCGG 1 cut(s) 579
SfuI TTCGAA 2 cut(s) 431, 1092
SgrAI CRCCGGYG 1 cut(s) 257
SgrBI CCGCGG 1 cut(s) 579
SinI GGWCC 2 cut(s) 373, 1307
SmlI CTYRAG 1 cut(s) 1151
SmoI CTYRAG 1 cut(s) 1151
SseBI AGGCCT 1 cut(s) 1465
SsiI CCGC 7 cut(s) 156, 242, 264, 576, 578, 707, 1259
SspDI GGCGCC 1 cut(s) 260
SspMI CTAG 2 cut(s) 1473, 1907
SstI GAGCTC 2 cut(s) 145, 620
StuI AGGCCT 1 cut(s) 1465
StyD4I CCNGG 3 cut(s) 369, 377, 1548
StyI CCWWGG 3 cut(s) 352, 402, 1935
TaaI ACNGT 9 cut(s) 15, 228, 455, 1014, 1056, 1484, 1539, 1627, 1726
TaiI ACGT 6 cut(s) 44, 312, 765, 1889, 2054, 2245
TaqI TCGA 7 cut(s) 84, 230, 318, 417, 431, 569, 1092
TaqII GACCGA 1 cut(s) 308
TatI WGTACW 3 cut(s) 387, 1005, 1117
TauI GCSGC 3 cut(s) 266, 709, 1262
TfiI GAWTC 2 cut(s) 623, 1403
Tru1I TTAA 9 cut(s) 816, 854, 1152, 1571, 1643, 1788, 2103, 2150, 2263
Tru9I TTAA 9 cut(s) 816, 854, 1152, 1571, 1643, 1788, 2103, 2150, 2263
TscAI CASTG 3 cut(s) 59, 67, 206
TseFI GTSAC 1 cut(s) 1451
TseI GCWGC 2 cut(s) 75, 1556
Tsp45I GTSAC 1 cut(s) 1451
TspGWI ACGGA 4 cut(s) 139, 248, 1700, 2199
TspRI CASTG 3 cut(s) 59, 67, 206
Vha464I CTTAAG 1 cut(s) 1151
VpaK11BI GGWCC 2 cut(s) 373, 1307
VspI ATTAAT 1 cut(s) 1571
XagI CCTNNNNNAGG 1 cut(s) 1550
XapI RAATTY 7 cut(s) 357, 534, 571, 1020, 1064, 1659, 1781
XcmI CCANNNNNNNNNTGG 1 cut(s) 1595
XmiI GTMKAC 2 cut(s) 230, 982
XmnI GAANNNNTTC 1 cut(s) 437
XspI CTAG 2 cut(s) 1473, 1907
ZraI GACGTC 1 cut(s) 310
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.