pycom17g20230

isoform X1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0005735
Physical Location & Seq
Forward (+)
18398112 .. 18403389
5278 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 2061 bp
ATGGCTTCTGTGGGTAAGGATGATGTGGAGTATGATAGTGATCCGGAGGTAACGAAGCGATCTCTGGCGATGCGGAGGAGGGAAGCGGCCAGCGACGATGAGGAAGGTGAAGAGGAGGCGAGGGAGAAACCGAGGAAGGATCCGAGGGCCGCGGTTCACTCCGATGAATCGGATGATCAGGGTGGGGCGGCGGAATATGATGATGACGAAGAACTAGAAGAAGAAGAAGGAGAAGAAGAGGAAGAGATTGATGAAGAAGAAGAGTTTGAGGAGGAAGTTGTGGAAGTTGTGGAAGAAGAGGAGTTGGAAAGATATCAGGAGAGAGGCCGGGAAGGCGAGATGGGTGGCAGGGTTGAGGTTTTGGCTGTTAAGGAATCGAGCGGTGATGGTCGGAGGTCTGTCGAAGGGTCCGTGGATGAGAATGGGGGGAATCAGGCTGAAGAAGGGGAGGAGAAGAAGGAGAATGAACCATTTGCAGTCCCCACAGCGGGTGCTTTTTATATGCATGATGACCGCTTCAGGGAAAACTCTGGTGGTCGGCACAGATCTTTTTTCATCTCTCATCTTTCTGATGTGCACTACTTACTAATTCAGGGAAAGAAACCTTCAAAGGGTCCTTATGTAGGTCGTGGAAGAAACCGAGGTATTGATCGTGGGAGTGCCAGAGGAAATAGGTCTAAAGAATATGACAGGTCTAAAGAATATGACAATAATAATCAGAGACAGGTACCCAAGGGTGTTAGAGGGAGAGGGCCCAGGAGGTCTGGAAAGCCTGCTGAGAAAAACATACAAAGTAATTTAGCGAGAACATTTACTCCCAACTCAAATGCAGAGTCTGGCCAGGTTTCTGCTAGGAAAAATTCCTTTGCCTCAAGCTTGAATTCAGCTTCTCCTCCTTTTTACCCATCAGGCTCTTCCAGTTCCAATAAAGGTATAACTCTTACTCCTAAGCGGGATGCACAGGTTGGGAGTGGTAAGAGGAACCTTCGGTCTTCTGTAGTGCATGAGACTTTCAATGTGCCCCAAACCAATGCATTGGAGCGAGGTAAGAACATAGCTGAATCTGTCGGTATGGACAAGCTTTATATAAATGACTCTACCAATCCAGCTGCTGGGAAGACTTTGACCAACATAAAAATGTCACCTTCTGGGTCATCATTTGTCAATACTACTCAAGCTCCTCAGTCTAGGGTTCAAGGAAGGGGTGTTATCTCAGGACAAATGAGTTATCAACCAGTGTCTCAGAACCAAATGACAAGGGCTTCTCCATCCACACATCTTCATGCTGTTCAGCGCAATCCTGCTCAAAATCGATCTCAACTGGCTCTGCAAGGTCCTGCTCAGCAGATGGGACAACGTCCTGGAAGTGGATCTCAGGCTTCCTCTCCACCCAAAACAACCATGTCATCAAATTCCTTCGAATCAGGAGAAGTTGAATCACCTTCAGATTCAAGTAAATCTAAAGGTGCCCTGGCTGCAAAGGGAAAAGGCAGTGTTCAGGGTAGTGCAAGGGGCAGTATTACTTATGGTGGAGCTCCAGTTACGGTATCCACTGGGAGTATGGGTGTTAATCATGATCAGAACTTCCCTGGTGCTCCAGCCTTTTTGCCTGTCATGCAATTTGGGGGCCAGCATACTGGGGGTATGGGAGTTCCTGCTGTTGGCATGGCGTTCCCTGGATATGTTGCTCAGCCACAACTTGGTGGTTTAGGAAATTCTGGCGAAATGACATGGTTACCTGTATTGGCGGGTGCTGCAGGAGCATTGGGGGCAACATATTGTCCTCCATATATTACAGTTGACGGTTCTTATCATGGTCGCCCATCTGGACAAACATCTTCTGTGGGTTCCCTGAGGCCTTCGTCTGAGCCCTTTGTGATCTTCAGCCTATGTTCTGGGGCACTTTGGGCCAATGTTTTCGAAGATATCTTAGTTACGTATGACAAACACCTGCTCATGCTGCACTTAATCGAAGATCAATATTGCGGCTTGACGGGTGCCATTCCTTGTTTACAATTTCGGTTTTCTTGTGGTTTGGTTTTTATCGAAAAGCTTGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Btz

Protein Analysis

687

Amino Acids

73.81

Weight (kDa)

5.31

Isoelectric Point (pI)

60.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Btz PF09405 139 - 184 1.3e-10 CASC3/Barentsz eIF4AIII binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1959
Acc36I ACCTGC 1 cut(s) 1959
Acc65I GGTACC 1 cut(s) 727
AccB1I GGYRCC 3 cut(s) 727, 1466, 1997
AccB7I CCANNNNNTGG 2 cut(s) 1112, 1700
AccBSI CCGCTC 1 cut(s) 381
AccII CGCG 1 cut(s) 152
AccIII TCCGGA 1 cut(s) 43
AclWI GGATC 4 cut(s) 35, 134, 147, 1378
AcoI YGGCCR 2 cut(s) 87, 838
AcsI RAATTY 4 cut(s) 859, 880, 1411, 1714
AcuI CTGAAG 4 cut(s) 459, 502, 1428, 1867
AfaI GTAC 1 cut(s) 729
AfiI CCNNNNNNNGG 9 cut(s) 487, 488, 520, 611, 623, 1112, 1367, 1661, 1700
AgsI TTSAA 6 cut(s) 609, 880, 1015, 1196, 1436, 1452
AjnI CCWGG 6 cut(s) 755, 840, 1360, 1470, 1588, 1675
AloI GAACNNNNNNTCC 2 cut(s) 799, 831
AluBI AGCT 8 cut(s) 876, 887, 1058, 1081, 1109, 1178, 1535, 2053
AluI AGCT 8 cut(s) 876, 887, 1058, 1081, 1109, 1178, 1535, 2053
Alw21I GWGCWC 3 cut(s) 579, 1537, 1597
Alw26I GTCTC 3 cut(s) 715, 1001, 1245
Alw44I GTGCAC 1 cut(s) 575
AlwI GGATC 4 cut(s) 35, 134, 147, 1378
AlwNI CAGNNNCTG 2 cut(s) 836, 1112
Aor13HI TCCGGA 1 cut(s) 43
AoxI GGCC 8 cut(s) 87, 147, 325, 752, 838, 1627, 1856, 1908
ApaI GGGCCC 1 cut(s) 756
ApaLI GTGCAC 1 cut(s) 575
ApeKI GCWGC 4 cut(s) 1109, 1475, 1754, 1960
ApoI RAATTY 4 cut(s) 859, 880, 1411, 1714
ArsI GACNNNNNNTTYG 2 cut(s) 818, 850
Asp718I GGTACC 1 cut(s) 727
AspLEI GCGC 1 cut(s) 1296
AspS9I GGNCC 8 cut(s) 147, 408, 614, 752, 753, 1334, 1627, 1908
AsuC2I CCSGG 1 cut(s) 329
AsuHPI GGTGA 4 cut(s) 119, 395, 1134, 1431
AsuII TTCGAA 2 cut(s) 1419, 1920
AvaII GGWCC 3 cut(s) 408, 614, 1334
AxyI CCTNAGG 1 cut(s) 1853
BaeGI GKGCMC 5 cut(s) 579, 756, 1023, 1471, 1903
BalI TGGCCA 1 cut(s) 840
BamHI GGATCC 1 cut(s) 139
BanI GGYRCC 3 cut(s) 727, 1466, 1997
BanII GRGCYC 3 cut(s) 756, 1537, 1872
BbsI GAAGAC 2 cut(s) 984, 1124
Bbv12I GWGCWC 3 cut(s) 579, 1537, 1597
BbvI GCAGC 4 cut(s) 1096, 1462, 1741, 1947
BccI CCATC 6 cut(s) 334, 380, 913, 1276, 1342, 1831
BciT130I CCWGG 6 cut(s) 757, 842, 1362, 1472, 1590, 1677
BciVI GTATCC 1 cut(s) 1558
BclI TGATCA 2 cut(s) 175, 1576
BcnI CCSGG 1 cut(s) 329
BcoDI GTCTC 3 cut(s) 715, 1001, 1245
BfaI CTAG 3 cut(s) 215, 852, 1188
BfmI CTRYAG 2 cut(s) 996, 1755
BfuAI ACCTGC 1 cut(s) 1959
BfuI GTATCC 1 cut(s) 1558
BglI GCCNNNNNGGC 1 cut(s) 333
BglII AGATCT 1 cut(s) 545
BisI GCNGC 8 cut(s) 87, 150, 189, 1110, 1476, 1755, 1961, 1987
BlpI GCTNAGC 2 cut(s) 1341, 1689
BlsI GCNGC 8 cut(s) 88, 151, 190, 1111, 1477, 1756, 1962, 1988
Bme1390I CCNGG 7 cut(s) 329, 757, 842, 1362, 1472, 1590, 1677
Bme18I GGWCC 3 cut(s) 408, 614, 1334
BmgT120I GGNCC 8 cut(s) 147, 408, 614, 752, 753, 1334, 1627, 1908
BmrFI CCNGG 7 cut(s) 329, 757, 842, 1362, 1472, 1590, 1677
BmrI ACTGGG 2 cut(s) 1563, 1647
BmsI GCATC 2 cut(s) 60, 946
BmuI ACTGGG 2 cut(s) 1563, 1647
BpiI GAAGAC 2 cut(s) 984, 1124
BpmI CTGGAG 2 cut(s) 1521, 1581
Bpu10I CCTNAGC 1 cut(s) 948
Bpu1102I GCTNAGC 2 cut(s) 1341, 1689
Bpu14I TTCGAA 2 cut(s) 1419, 1920
BpuEI CTTGAG 2 cut(s) 856, 1158
BpuMI CCSGG 1 cut(s) 329
Bsa29I ATCGAT 1 cut(s) 1312
BsaAI YACGTR 1 cut(s) 1938
BsaBI GATNNNNATC 1 cut(s) 39
BsaWI WCCGGW 1 cut(s) 43
Bsc4I CCNNNNNNNGG 9 cut(s) 487, 488, 520, 611, 623, 1112, 1367, 1661, 1700
Bse1I ACTGG 6 cut(s) 918, 1235, 1326, 1538, 1558, 1642
Bse21I CCTNAGG 1 cut(s) 1853
Bse8I GATNNNNATC 1 cut(s) 39
BseAI TCCGGA 1 cut(s) 43
BseBI CCWGG 6 cut(s) 757, 842, 1362, 1472, 1590, 1677
BseCI ATCGAT 1 cut(s) 1312
BseGI GGATG 5 cut(s) 25, 178, 421, 961, 1268
BseJI GATNNNNATC 1 cut(s) 39
BseLI CCNNNNNNNGG 9 cut(s) 487, 488, 520, 611, 623, 1112, 1367, 1661, 1700
BseMII CTCAG 9 cut(s) 768, 1196, 1227, 1256, 1355, 1388, 1703, 1844, 1857
BseNI ACTGG 6 cut(s) 918, 1235, 1326, 1538, 1558, 1642
BseRI GAGGAG 7 cut(s) 91, 128, 284, 314, 464, 882, 1170
BseSI GKGCMC 5 cut(s) 579, 756, 1023, 1471, 1903
BseXI GCAGC 4 cut(s) 1096, 1462, 1741, 1947
BseYI CCCAGC 1 cut(s) 1112
BsgI GTGCAG 1 cut(s) 1946
Bsh1236I CGCG 1 cut(s) 152
BshFI GGCC 8 cut(s) 89, 149, 327, 754, 840, 1629, 1858, 1910
BshNI GGYRCC 3 cut(s) 727, 1466, 1997
BshVI ATCGAT 1 cut(s) 1312
BsiHKAI GWGCWC 3 cut(s) 579, 1537, 1597
BsiSI CCGG 2 cut(s) 44, 328
BslFI GGGAC 2 cut(s) 464, 1365
BslI CCNNNNNNNGG 9 cut(s) 487, 488, 520, 611, 623, 1112, 1367, 1661, 1700
BsmAI GTCTC 3 cut(s) 715, 1001, 1245
BsmFI GGGAC 2 cut(s) 464, 1365
BsnI GGCC 8 cut(s) 89, 149, 327, 754, 840, 1629, 1858, 1910
Bsp119I TTCGAA 2 cut(s) 1419, 1920
Bsp120I GGGCCC 1 cut(s) 752
Bsp1286I GDGCHC 8 cut(s) 579, 756, 1023, 1471, 1537, 1597, 1872, 1903
Bsp13I TCCGGA 1 cut(s) 43
Bsp1720I GCTNAGC 2 cut(s) 1341, 1689
BspANI GGCC 8 cut(s) 89, 149, 327, 754, 840, 1629, 1858, 1910
BspCNI CTCAG 9 cut(s) 769, 1195, 1226, 1255, 1354, 1387, 1702, 1845, 1858
BspDI ATCGAT 1 cut(s) 1312
BspEI TCCGGA 1 cut(s) 43
BspFNI CGCG 1 cut(s) 152
BspHI TCATGA 1 cut(s) 1573
BspMAI CTGCAG 1 cut(s) 1759
BspMI ACCTGC 1 cut(s) 1959
BspPI GGATC 4 cut(s) 35, 134, 147, 1378
BspQI GCTCTTC 1 cut(s) 919
BspT104I TTCGAA 2 cut(s) 1419, 1920
BspT107I GGYRCC 3 cut(s) 727, 1466, 1997
BsrBI CCGCTC 1 cut(s) 381
BsrI ACTGG 6 cut(s) 918, 1235, 1326, 1538, 1558, 1642
BssT1I CCWWGG 1 cut(s) 732
Bst2UI CCWGG 6 cut(s) 757, 842, 1362, 1472, 1590, 1677
Bst4CI ACNGT 3 cut(s) 1546, 1798, 1805
Bst6I CTCTTC 6 cut(s) 105, 231, 237, 255, 291, 919
BstBAI YACGTR 1 cut(s) 1938
BstBI TTCGAA 2 cut(s) 1419, 1920
BstC8I GCNNGC 3 cut(s) 91, 774, 1631
BstDSI CCRYGG 2 cut(s) 150, 411
BstEII GGTNACC 1 cut(s) 1734
BstENI CCTNNNNNAGG 1 cut(s) 621
BstF5I GGATG 5 cut(s) 25, 178, 421, 961, 1268
BstFNI CGCG 1 cut(s) 152
BstHHI GCGC 1 cut(s) 1296
BstMAI GTCTC 3 cut(s) 715, 1001, 1245
BstMWI GCNNNNNNNGC 8 cut(s) 333, 1475, 1615, 1754, 1760, 1769, 1907, 1960
BstNI CCWGG 6 cut(s) 757, 842, 1362, 1472, 1590, 1677
BstPI GGTNACC 1 cut(s) 1734
BstSCI CCNGG 7 cut(s) 327, 755, 840, 1360, 1470, 1588, 1675
BstSFI CTRYAG 2 cut(s) 996, 1755
BstSLI GKGCMC 5 cut(s) 579, 756, 1023, 1471, 1903
BstSNI TACGTA 1 cut(s) 1938
BstUI CGCG 1 cut(s) 152
BstV1I GCAGC 4 cut(s) 1096, 1462, 1741, 1947
BstV2I GAAGAC 2 cut(s) 984, 1124
BstX2I RGATCY 3 cut(s) 139, 545, 1370
BstXI CCANNNNNNTGG 2 cut(s) 1036, 1637
BstYI RGATCY 3 cut(s) 139, 545, 1370
Bsu15I ATCGAT 1 cut(s) 1312
Bsu36I CCTNAGG 1 cut(s) 1853
BsuI GTATCC 1 cut(s) 1558
BsuRI GGCC 8 cut(s) 89, 149, 327, 754, 840, 1629, 1858, 1910
BsuTUI ATCGAT 1 cut(s) 1312
BtgI CCRYGG 2 cut(s) 150, 411
BtgZI GCGATG 1 cut(s) 83
BtsCI GGATG 5 cut(s) 25, 178, 421, 961, 1268
BtsI GCAGTG 1 cut(s) 1498
BtsIMutI CAGTG 3 cut(s) 1242, 1498, 1551
BveI ACCTGC 1 cut(s) 1959
Cac8I GCNNGC 3 cut(s) 91, 774, 1631
CaiI CAGNNNCTG 2 cut(s) 836, 1112
CciI TCATGA 1 cut(s) 1573
CfoI GCGC 1 cut(s) 1296
Cfr13I GGNCC 8 cut(s) 147, 408, 614, 752, 753, 1334, 1627, 1908
Cfr42I CCGCGG 1 cut(s) 153
ClaI ATCGAT 1 cut(s) 1312
Csp6I GTAC 1 cut(s) 728
CviQI GTAC 1 cut(s) 728
EaeI YGGCCR 2 cut(s) 87, 838
Eam1104I CTCTTC 6 cut(s) 105, 231, 237, 255, 291, 919
EarI CTCTTC 6 cut(s) 105, 231, 237, 255, 291, 919
EciI GGCGGA 1 cut(s) 206
Ecl136II GAGCTC 1 cut(s) 1535
Eco105I TACGTA 1 cut(s) 1938
Eco130I CCWWGG 1 cut(s) 732
Eco147I AGGCCT 1 cut(s) 1858
Eco24I GRGCYC 3 cut(s) 756, 1537, 1872
Eco32I GATATC 2 cut(s) 314, 1927
Eco47I GGWCC 3 cut(s) 408, 614, 1334
Eco53kI GAGCTC 1 cut(s) 1535
Eco57I CTGAAG 4 cut(s) 459, 502, 1428, 1867
Eco81I CCTNAGG 1 cut(s) 1853
Eco91I GGTNACC 1 cut(s) 1734
EcoICRI GAGCTC 1 cut(s) 1535
EcoNI CCTNNNNNAGG 1 cut(s) 621
EcoO109I RGGNCCY 3 cut(s) 614, 752, 1334
EcoO65I GGTNACC 1 cut(s) 1734
EcoRI GAATTC 1 cut(s) 880
EcoRII CCWGG 6 cut(s) 755, 840, 1360, 1470, 1588, 1675
EcoRV GATATC 2 cut(s) 314, 1927
EcoT14I CCWWGG 1 cut(s) 732
EcoT22I ATGCAT 2 cut(s) 507, 1036
EcoT38I GRGCYC 3 cut(s) 756, 1537, 1872
ErhI CCWWGG 1 cut(s) 732
FaqI GGGAC 2 cut(s) 464, 1365
FauI CCCGC 3 cut(s) 481, 945, 1741
FbaI TGATCA 2 cut(s) 175, 1576
Fnu4HI GCNGC 8 cut(s) 87, 150, 189, 1110, 1476, 1755, 1961, 1987
FokI GGATG 5 cut(s) 32, 185, 428, 968, 1255
FriOI GRGCYC 3 cut(s) 756, 1537, 1872
Fsp4HI GCNGC 8 cut(s) 87, 150, 189, 1110, 1476, 1755, 1961, 1987
FspBI CTAG 3 cut(s) 215, 852, 1188
GlaI GCGC 1 cut(s) 1295
GluI GCNGC 8 cut(s) 87, 150, 189, 1110, 1476, 1755, 1961, 1987
GsaI CCCAGC 1 cut(s) 1116
GsuI CTGGAG 2 cut(s) 1521, 1581
HaeIII GGCC 8 cut(s) 89, 149, 327, 754, 840, 1629, 1858, 1910
HapII CCGG 2 cut(s) 44, 328
HhaI GCGC 1 cut(s) 1296
Hin6I GCGC 1 cut(s) 1294
HinP1I GCGC 1 cut(s) 1294
HincII GTYRAC 1 cut(s) 1801
HindII GTYRAC 1 cut(s) 1801
HindIII AAGCTT 3 cut(s) 874, 1079, 2051
HinfI GANTC 9 cut(s) 167, 374, 430, 833, 1061, 1094, 1421, 1436, 1448
HpaII CCGG 2 cut(s) 44, 328
HphI GGTGA 4 cut(s) 119, 395, 1134, 1431
Hpy166II GTNNAC 4 cut(s) 157, 577, 1801, 2012
Hpy188III TCNNGA 7 cut(s) 44, 317, 765, 1215, 1425, 1574, 1827
Hpy8I GTNNAC 4 cut(s) 157, 577, 1801, 2012
Hpy99I CGWCG 1 cut(s) 98
HpyCH4III ACNGT 3 cut(s) 1546, 1798, 1805
HpyCH4IV ACGT 2 cut(s) 1357, 1937
HpyF10VI GCNNNNNNNGC 8 cut(s) 333, 1475, 1615, 1754, 1760, 1769, 1907, 1960
HpySE526I ACGT 2 cut(s) 1357, 1937
HspAI GCGC 1 cut(s) 1294
Kpn2I TCCGGA 1 cut(s) 43
KpnI GGTACC 1 cut(s) 731
Ksp22I TGATCA 2 cut(s) 175, 1576
KspI CCGCGG 1 cut(s) 153
LguI GCTCTTC 1 cut(s) 919
LmnI GCTCC 6 cut(s) 1039, 1183, 1532, 1540, 1600, 1760
Lsp1109I GCAGC 4 cut(s) 1096, 1462, 1741, 1947
LweI GCATC 2 cut(s) 60, 946
MaeI CTAG 3 cut(s) 215, 852, 1188
MaeII ACGT 2 cut(s) 1357, 1937
MaeIII GTNAC 5 cut(s) 49, 1140, 1540, 1734, 1933
MbiI CCGCTC 1 cut(s) 381
MflI RGATCY 3 cut(s) 139, 545, 1370
MhlI GDGCHC 8 cut(s) 579, 756, 1023, 1471, 1537, 1597, 1872, 1903
MlsI TGGCCA 1 cut(s) 840
MluCI AATT 8 cut(s) 588, 796, 859, 880, 1411, 1619, 1714, 2015
MluNI TGGCCA 1 cut(s) 840
MlyI GAGTC 2 cut(s) 842, 1088
MmeI TCCRAC 2 cut(s) 285, 371
Mox20I TGGCCA 1 cut(s) 840
Mph1103I ATGCAT 2 cut(s) 507, 1036
MroI TCCGGA 1 cut(s) 43
MscI TGGCCA 1 cut(s) 840
MseI TTAA 3 cut(s) 369, 1569, 1967
MslI CAYNNNNRTG 3 cut(s) 162, 1136, 1281
Msp20I TGGCCA 1 cut(s) 840
MspA1I CMGCKG 3 cut(s) 152, 488, 1109
MspI CCGG 2 cut(s) 44, 328
MspR9I CCNGG 7 cut(s) 329, 757, 842, 1362, 1472, 1590, 1677
MvaI CCWGG 6 cut(s) 757, 842, 1362, 1472, 1590, 1677
MvnI CGCG 1 cut(s) 152
MwoI GCNNNNNNNGC 8 cut(s) 333, 1475, 1615, 1754, 1760, 1769, 1907, 1960
NciI CCSGG 1 cut(s) 329
NmuCI GTSAC 1 cut(s) 1140
NsiI ATGCAT 2 cut(s) 507, 1036
NspV TTCGAA 2 cut(s) 1419, 1920
PagI TCATGA 1 cut(s) 1573
PaqCI CACCTGC 1 cut(s) 1959
PceI AGGCCT 1 cut(s) 1858
PciSI GCTCTTC 1 cut(s) 919
PcsI WCGNNNNNNNCGW 1 cut(s) 408
PfeI GAWTC 7 cut(s) 167, 374, 430, 1061, 1421, 1436, 1448
PflFI GACNNNGTC 1 cut(s) 1356
PflMI CCANNNNNTGG 2 cut(s) 1112, 1700
PfoI TCCNGGA 1 cut(s) 1360
PkrI GCNGC 8 cut(s) 88, 151, 190, 1111, 1477, 1756, 1962, 1988
PleI GAGTC 2 cut(s) 841, 1088
PpsI GAGTC 2 cut(s) 841, 1088
Ppu21I YACGTR 1 cut(s) 1938
PpuMI RGGWCCY 2 cut(s) 614, 1334
Psp124BI GAGCTC 1 cut(s) 1537
Psp5II RGGWCCY 2 cut(s) 614, 1334
Psp6I CCWGG 6 cut(s) 755, 840, 1360, 1470, 1588, 1675
PspEI GGTNACC 1 cut(s) 1734
PspFI CCCAGC 1 cut(s) 1112
PspGI CCWGG 6 cut(s) 755, 840, 1360, 1470, 1588, 1675
PspOMI GGGCCC 1 cut(s) 752
PspPI GGNCC 8 cut(s) 147, 408, 614, 752, 753, 1334, 1627, 1908
PspPPI RGGWCCY 2 cut(s) 614, 1334
PstI CTGCAG 1 cut(s) 1759
PstNI CAGNNNCTG 2 cut(s) 836, 1112
PsuI RGATCY 3 cut(s) 139, 545, 1370
PsyI GACNNNGTC 1 cut(s) 1356
PvuII CAGCTG 1 cut(s) 1109
RsaI GTAC 1 cut(s) 729
RsaNI GTAC 1 cut(s) 728
RseI CAYNNNNRTG 3 cut(s) 162, 1136, 1281
SacI GAGCTC 1 cut(s) 1537
SacII CCGCGG 1 cut(s) 153
SapI GCTCTTC 1 cut(s) 919
SaqAI TTAA 3 cut(s) 369, 1569, 1967
SatI GCNGC 8 cut(s) 87, 150, 189, 1110, 1476, 1755, 1961, 1987
Sau96I GGNCC 8 cut(s) 147, 408, 614, 752, 753, 1334, 1627, 1908
SchI GAGTC 2 cut(s) 842, 1088
ScrFI CCNGG 7 cut(s) 329, 757, 842, 1362, 1472, 1590, 1677
SduI GDGCHC 8 cut(s) 579, 756, 1023, 1471, 1537, 1597, 1872, 1903
SfaNI GCATC 2 cut(s) 60, 946
SfcI CTRYAG 2 cut(s) 996, 1755
Sfr303I CCGCGG 1 cut(s) 153
SfuI TTCGAA 2 cut(s) 1419, 1920
SgrBI CCGCGG 1 cut(s) 153
SinI GGWCC 3 cut(s) 408, 614, 1334
SmiMI CAYNNNNRTG 3 cut(s) 162, 1136, 1281
SmlI CTYRAG 2 cut(s) 871, 1173
SmoI CTYRAG 2 cut(s) 871, 1173
SnaBI TACGTA 1 cut(s) 1938
Sse9I AATT 8 cut(s) 588, 796, 859, 880, 1411, 1619, 1714, 2015
SseBI AGGCCT 1 cut(s) 1858
SspI AATATT 1 cut(s) 1982
SspMI CTAG 3 cut(s) 215, 852, 1188
SstI GAGCTC 1 cut(s) 1537
StuI AGGCCT 1 cut(s) 1858
StyD4I CCNGG 7 cut(s) 327, 755, 840, 1360, 1470, 1588, 1675
StyI CCWWGG 1 cut(s) 732
TaaI ACNGT 3 cut(s) 1546, 1798, 1805
TaiI ACGT 2 cut(s) 1360, 1940
TaqI TCGA 7 cut(s) 377, 402, 1312, 1419, 1920, 1971, 2046
TaqII GACCGA 1 cut(s) 978
TasI AATT 8 cut(s) 588, 796, 859, 880, 1411, 1619, 1714, 2015
TauI GCSGC 4 cut(s) 89, 152, 191, 1989
TfiI GAWTC 7 cut(s) 167, 374, 430, 1061, 1421, 1436, 1448
Tru1I TTAA 3 cut(s) 369, 1569, 1967
Tru9I TTAA 3 cut(s) 369, 1569, 1967
TscAI CASTG 3 cut(s) 1242, 1498, 1558
TseFI GTSAC 1 cut(s) 1140
TseI GCWGC 4 cut(s) 1109, 1475, 1754, 1960
Tsp45I GTSAC 1 cut(s) 1140
TspDTI ATGAA 5 cut(s) 180, 267, 480, 544, 1271
TspGWI ACGGA 1 cut(s) 400
TspRI CASTG 3 cut(s) 1242, 1498, 1558
Tth111I GACNNNGTC 1 cut(s) 1356
Van91I CCANNNNNTGG 2 cut(s) 1112, 1700
VneI GTGCAC 1 cut(s) 575
VpaK11BI GGWCC 3 cut(s) 408, 614, 1334
XagI CCTNNNNNAGG 1 cut(s) 621
XapI RAATTY 4 cut(s) 859, 880, 1411, 1714
XcmI CCANNNNNNNNNTGG 1 cut(s) 1558
XspI CTAG 3 cut(s) 215, 852, 1188
Zsp2I ATGCAT 2 cut(s) 507, 1036
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.