pycom17g20850

Zinc finger CCCH domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0006416
Physical Location & Seq
Forward (+)
19286497 .. 19287975
1479 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom17g20850.1

Sequence Viewer

Length: 813 bp
ATGAGTACTCCATTACCTACTCCCCAATTTTTTGCTCAATATCTTGAATCCGGTGACAGCGGGCGTCAGATGAAACATCAAATTCCCACTGAGGAGAAAGAAATCAAAGCTCAATCTGACATCAACTCCCAAGCTTTCAAGAAACCCAGAACTTCGGAAGCTGTATCGAACTCGAATGCGGCCTCAGGACAGAACAAAATGTTGCCTAGCTATTACAAGACCCAACTGTGCCGTAAATTCCAAATGGGCTGTTGCTTTTCTGGGCAGAGATGTTCCTTTGCTCATGGCACCAGTGATCTCCGCAGGACTTTGCGTGATGCGCAAGGAATGGAAACTGAGAAGGGAAACTTGGTCAGGAGGACTTGGAATGGTGATCATGGAAGTTCTAATGGAGTGAGAATATGCAGGTCGTTCTTTAGATGGGGAACATGCAGTTACGGAGACAAATGCCGCTTTCGTCATGTCAGTCCTGAAAATATCAGAGATAGTTCGTTCATAAGCATTTCAACTGCTGGTGGGTCTGCTCAATTTGACTGCAAGAAGTCTATGGAACTAAGGAAGCTTTGTGGCTATACTACACCAGAATGCGGAAGTGTGCAGGCTAACAGTTTAACGACTTATATGAGGGGCAACAATGCATCAAATCGTAGGATGGGGATTGCGACTGCTTATAAACAAGGTCAGAGTACGCAGTGCAACTTAGAGTGGAATGAACTTGAGAAATTGAGTCGCATCTATGCTGATTGGATTGAAGATATGCCTCTTCTACATGGCTCATCAAACAAAGTGGAGTGCTACTCCAGTTTAGTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-CCCH

Protein Analysis

271

Amino Acids

30.34

Weight (kDa)

9.14

Isoelectric Point (pI)

49.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-CCCH PF00642 132 - 155 2.3e-06 Zinc finger C-x8-C-x5-C-x3-H type (and similar)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 672
Acc16I TGCGCA 1 cut(s) 321
Acc36I ACCTGC 1 cut(s) 396
AccB1I GGYRCC 1 cut(s) 287
AciI CCGC 5 cut(s) 60, 179, 301, 451, 588
AcsI RAATTY 2 cut(s) 81, 236
AcyI GRCGYC 1 cut(s) 64
AfaI GTAC 2 cut(s) 7, 688
AfiI CCNNNNNNNGG 1 cut(s) 587
AgsI TTSAA 4 cut(s) 47, 139, 507, 752
AjuI GAANNNNNNNTTGG 2 cut(s) 332, 364
AluBI AGCT 5 cut(s) 110, 134, 161, 210, 562
AluI AGCT 5 cut(s) 110, 134, 161, 210, 562
Alw26I GTCTC 1 cut(s) 435
AoxI GGCC 1 cut(s) 180
ApoI RAATTY 2 cut(s) 81, 236
AspLEI GCGC 1 cut(s) 322
AsuHPI GGTGA 2 cut(s) 65, 383
AxyI CCTNAGG 1 cut(s) 184
BanI GGYRCC 1 cut(s) 287
BccI CCATC 2 cut(s) 414, 646
BceAI ACGGC 1 cut(s) 216
BclI TGATCA 1 cut(s) 373
BcoDI GTCTC 1 cut(s) 435
BfaI CTAG 1 cut(s) 207
BfuAI ACCTGC 1 cut(s) 396
BisI GCNGC 2 cut(s) 180, 451
BlsI GCNGC 2 cut(s) 181, 452
BmcAI AGTACT 1 cut(s) 7
BmiI GGNNCC 1 cut(s) 289
BmsI GCATC 3 cut(s) 307, 647, 741
BplI GAGNNNNNCTC 1 cut(s) 782
BpmI CTGGAG 1 cut(s) 784
BpuEI CTTGAG 1 cut(s) 737
BsaHI GRCGYC 1 cut(s) 64
BsaWI WCCGGW 1 cut(s) 50
BsaXI ACNNNNNCTCC 2 cut(s) 110, 140
Bsc4I CCNNNNNNNGG 1 cut(s) 587
Bse1I ACTGG 2 cut(s) 291, 801
Bse21I CCTNAGG 1 cut(s) 184
BseGI GGATG 1 cut(s) 657
BseLI CCNNNNNNNGG 1 cut(s) 587
BseMII CTCAG 3 cut(s) 81, 198, 327
BseNI ACTGG 2 cut(s) 291, 801
BseRI GAGGAG 1 cut(s) 107
BsgI GTGCAG 1 cut(s) 617
BshFI GGCC 1 cut(s) 182
BshNI GGYRCC 1 cut(s) 287
BsiSI CCGG 1 cut(s) 51
BslI CCNNNNNNNGG 1 cut(s) 587
BsmAI GTCTC 1 cut(s) 435
BsmI GAATGC 2 cut(s) 181, 590
BsnI GGCC 1 cut(s) 182
Bsp143I GATC 2 cut(s) 295, 373
BspACI CCGC 5 cut(s) 60, 179, 301, 451, 588
BspANI GGCC 1 cut(s) 182
BspCNI CTCAG 3 cut(s) 82, 197, 328
BspLI GGNNCC 1 cut(s) 289
BspMI ACCTGC 1 cut(s) 396
BspT107I GGYRCC 1 cut(s) 287
BsrI ACTGG 2 cut(s) 291, 801
BssMI GATC 2 cut(s) 295, 373
BssNI GRCGYC 1 cut(s) 64
Bst4CI ACNGT 2 cut(s) 228, 608
Bst6I CTCTTC 1 cut(s) 768
BstACI GRCGYC 1 cut(s) 64
BstC8I GCNNGC 2 cut(s) 62, 600
BstDEI CTNAG 5 cut(s) 90, 184, 336, 554, 700
BstF5I GGATG 1 cut(s) 657
BstHHI GCGC 1 cut(s) 322
BstKTI GATC 2 cut(s) 298, 376
BstMAI GTCTC 1 cut(s) 435
BstMBI GATC 2 cut(s) 295, 373
BstMWI GCNNNNNNNGC 1 cut(s) 319
BstNSI RCATGY 1 cut(s) 432
Bsu36I CCTNAGG 1 cut(s) 184
BsuRI GGCC 1 cut(s) 182
BtsCI GGATG 1 cut(s) 657
BtsI GCAGTG 1 cut(s) 698
BtsIMutI CAGTG 3 cut(s) 87, 298, 698
BveI ACCTGC 1 cut(s) 396
Cac8I GCNNGC 2 cut(s) 62, 600
CfoI GCGC 1 cut(s) 322
CseI GACGC 1 cut(s) 53
Csp6I GTAC 2 cut(s) 6, 687
CspCI CAANNNNNGTGG 2 cut(s) 768, 803
CviAII CATG 5 cut(s) 284, 377, 429, 461, 770
CviQI GTAC 2 cut(s) 6, 687
DdeI CTNAG 5 cut(s) 90, 184, 336, 554, 700
DpnI GATC 2 cut(s) 297, 375
DpnII GATC 2 cut(s) 295, 373
Eam1104I CTCTTC 1 cut(s) 768
EarI CTCTTC 1 cut(s) 768
Eco81I CCTNAGG 1 cut(s) 184
EcoT22I ATGCAT 1 cut(s) 640
FaeI CATG 5 cut(s) 287, 380, 432, 464, 773
FalI AAGNNNNNCTT 2 cut(s) 332, 364
FatI CATG 5 cut(s) 283, 376, 428, 460, 769
FauI CCCGC 1 cut(s) 53
FbaI TGATCA 1 cut(s) 373
Fnu4HI GCNGC 2 cut(s) 180, 451
FokI GGATG 1 cut(s) 664
Fsp4HI GCNGC 2 cut(s) 180, 451
FspBI CTAG 1 cut(s) 207
FspI TGCGCA 1 cut(s) 321
GlaI GCGC 1 cut(s) 321
GluI GCNGC 2 cut(s) 180, 451
GsuI CTGGAG 1 cut(s) 784
HaeIII GGCC 1 cut(s) 182
HapII CCGG 1 cut(s) 51
HgaI GACGC 1 cut(s) 53
HhaI GCGC 1 cut(s) 322
Hin1I GRCGYC 1 cut(s) 64
Hin1II CATG 5 cut(s) 287, 380, 432, 464, 773
Hin6I GCGC 1 cut(s) 320
HinP1I GCGC 1 cut(s) 320
HindIII AAGCTT 2 cut(s) 132, 560
HinfI GANTC 2 cut(s) 47, 727
HpaII CCGG 1 cut(s) 51
HphI GGTGA 2 cut(s) 65, 383
Hpy188I TCNGA 5 cut(s) 69, 118, 157, 482, 684
Hpy188III TCNNGA 5 cut(s) 44, 139, 186, 355, 470
HpyAV CCTTC 1 cut(s) 334
HpyCH4III ACNGT 2 cut(s) 228, 608
HpyCH4V TGCA 6 cut(s) 405, 432, 537, 598, 638, 696
HpyF10VI GCNNNNNNNGC 1 cut(s) 319
HpyF3I CTNAG 5 cut(s) 90, 184, 336, 554, 700
Hsp92I GRCGYC 1 cut(s) 64
Hsp92II CATG 5 cut(s) 287, 380, 432, 464, 773
HspAI GCGC 1 cut(s) 320
Ksp22I TGATCA 1 cut(s) 373
Kzo9I GATC 2 cut(s) 295, 373
LweI GCATC 3 cut(s) 307, 647, 741
MaeI CTAG 1 cut(s) 207
MaeIII GTNAC 2 cut(s) 53, 434
MalI GATC 2 cut(s) 297, 375
MboI GATC 2 cut(s) 295, 373
MboII GAAGA 2 cut(s) 755, 764
MluCI AATT 5 cut(s) 26, 81, 236, 527, 722
MlyI GAGTC 1 cut(s) 736
MnlI CCTC 5 cut(s) 85, 193, 351, 618, 771
Mph1103I ATGCAT 1 cut(s) 640
MseI TTAA 1 cut(s) 611
MslI CAYNNNNRTG 1 cut(s) 583
MspA1I CMGCKG 1 cut(s) 60
MspI CCGG 1 cut(s) 51
Mva1269I GAATGC 2 cut(s) 181, 590
MwoI GCNNNNNNNGC 1 cut(s) 319
NdeII GATC 2 cut(s) 295, 373
NlaIII CATG 5 cut(s) 287, 380, 432, 464, 773
NlaIV GGNNCC 1 cut(s) 289
NmuCI GTSAC 1 cut(s) 53
NsbI TGCGCA 1 cut(s) 321
NsiI ATGCAT 1 cut(s) 640
NspI RCATGY 1 cut(s) 432
PctI GAATGC 2 cut(s) 181, 590
PfeI GAWTC 1 cut(s) 47
PkrI GCNGC 2 cut(s) 181, 452
PleI GAGTC 1 cut(s) 735
PpsI GAGTC 1 cut(s) 735
PsiI TTATAA 1 cut(s) 672
PspN4I GGNNCC 1 cut(s) 289
RsaI GTAC 2 cut(s) 7, 688
RsaNI GTAC 2 cut(s) 6, 687
RseI CAYNNNNRTG 1 cut(s) 583
SaqAI TTAA 1 cut(s) 611
SatI GCNGC 2 cut(s) 180, 451
Sau3AI GATC 2 cut(s) 295, 373
ScaI AGTACT 1 cut(s) 7
SchI GAGTC 1 cut(s) 736
SetI ASST 8 cut(s) 19, 112, 136, 163, 212, 410, 564, 682
SfaNI GCATC 3 cut(s) 307, 647, 741
SmiMI CAYNNNNRTG 1 cut(s) 583
SmlI CTYRAG 1 cut(s) 716
SmoI CTYRAG 1 cut(s) 716
Sse9I AATT 5 cut(s) 26, 81, 236, 527, 722
SsiI CCGC 5 cut(s) 60, 179, 301, 451, 588
SspMI CTAG 1 cut(s) 207
TaaI ACNGT 2 cut(s) 228, 608
TaqI TCGA 2 cut(s) 167, 173
TasI AATT 5 cut(s) 26, 81, 236, 527, 722
TatI WGTACW 1 cut(s) 5
TauI GCSGC 2 cut(s) 182, 453
TfiI GAWTC 1 cut(s) 47
Tru1I TTAA 1 cut(s) 611
Tru9I TTAA 1 cut(s) 611
TscAI CASTG 3 cut(s) 94, 298, 698
TseFI GTSAC 1 cut(s) 53
Tsp45I GTSAC 1 cut(s) 53
TspDTI ATGAA 3 cut(s) 86, 484, 726
TspGWI ACGGA 1 cut(s) 453
TspRI CASTG 3 cut(s) 94, 298, 698
XapI RAATTY 2 cut(s) 81, 236
XceI RCATGY 1 cut(s) 432
XspI CTAG 1 cut(s) 207
ZrmI AGTACT 1 cut(s) 7
Zsp2I ATGCAT 1 cut(s) 640
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.