pycom17g20990

Belongs to the glycosyl hydrolase 28 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0009481
Physical Location & Seq
Forward (+)
19410011 .. 19413223
3213 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1389 bp
ATGAGAAGCCTTCGATCTTCCCCATTTGCTTCCAAACCCATAATTTATGGTGTTATTCATATGTTTTTTCTGTTAGCCAGCTCAAATACCGCGCATGGAGAAACCTTCGACTCCGTCTTGCAGCTCCTCCCACGACCCAGGTGGACAAGAATCCGACCCGGATCCAAGAGGGTCCTCTCTGTTGGCGAGTTTGGTGCAAAAGGAGATGGCGTCACTGATGACTCTCAGGCTTTTAAAAATGCTTGGGAGATAGCTTGTTCTATCCCTTTGCGGACAAGAATCTTAATTCCTGCTGGAAGCACTTACCTATTCCATCCTGTTGATATCGCTGGGCCTTGTCGATCAAAGGTGACTCTAATGATCTCTGGTACTGTCATTGCTCCTGAAGATCCTGATACTTGGATTGGTTTGAATCGACGTAAGTGGCTCTATTTCCATGGGGTGAACCACCTTACCGTAGAAGGGGGAGGGACAATCAATGGAATGGGGCAGGAATGGTGGGCTCGGTCTTGCAAAACCAACTCATCAAATCCATGTCGACATGCTCCAACGGCAGTTACGTTTCATAAGTGCAAGAATTTGAAGGTAAAGAATCTTTTGATTGTCAATAGTCAACAAATGCACATGGCATTCACAAACTGCATGCGGGTAGTGGCATCAAATCTCAGAGTGATAGCGCCTGCTGTTAGTCCTAATACTGATGGCATCCACATTAGTGCGTCGAAGGGTGTCGAGGTCAAGGATACCATAATAAGAACAGGAGACGACTGTATTTCTATAGTCAGCAATTCTTCACGAATCCGGATCATAAATATTGCTTGCGGCCCCGGTCATGGCATAAGCATTGGAAGCTTGGGAAAATCAAACTCTTGGTCCCAAGTGCATGATGTAGTGGTTGATACAGCATTCTTGTCCAACACAGATAATGGGGTGAGAATCAAAACATGGCAGGGCGGTAGTGGTTTTGCCTCCAACATTGCATTCCGGAATGTGTTGATGGAAAATGTATCAAATCCGATAATAATCGATCAGTATTATTGTGACTCCTGGCTGCCATGTCTAAATCAGACTTCGGCGGTAAAAGTGGAGAACATATCCTTCGTGCACATTAAAGGGACTTCTGCTACGGAGGAAGCAATAAAATTTGTTTGCAGTGACTATTCACCATGTGAAGGGTTATACATGGAAGATATTCAGCTTCTACCATCCGTTGGGGAGATCACCACCTCTCTTTGTTGGCAAGCTCAAGGTTCAAGTTTCGGTTTGGTAGACCCGCCCGCTTGCTTCTCGTGTAGCGAAAGCTTCATTACGCAAAAAGTCCCGTCATACTCTACCATTCATTCACAGAAGTTGGGCAGTTTGGAATATGGACGAACAGCAACCCAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005618 GO:0005623 GO:0009505 GO:0030312 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
Glyco_hydro_28

Protein Analysis

463

Amino Acids

50.44

Weight (kDa)

8.67

Isoelectric Point (pI)

42.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_28 PF00295 86 - 402 4.5e-98 Glycosyl hydrolases family 28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 1211
AccI GTMKAC 2 cut(s) 538, 1269
AccII CGCG 1 cut(s) 92
AccIII TCCGGA 2 cut(s) 801, 984
AciI CCGC 8 cut(s) 90, 271, 646, 822, 954, 1076, 1274, 1278
AclWI GGATC 4 cut(s) 156, 169, 383, 812
AcsI RAATTY 2 cut(s) 577, 1142
AcuI CTGAAG 1 cut(s) 405
AcyI GRCGYC 1 cut(s) 210
AdeI CACNNNGTG 1 cut(s) 1169
AfaI GTAC 1 cut(s) 370
AfiI CCNNNNNNNGG 4 cut(s) 462, 833, 1172, 1211
AgsI TTSAA 3 cut(s) 412, 583, 1254
AjnI CCWGG 2 cut(s) 137, 1046
AjuI GAANNNNNNNTTGG 2 cut(s) 965, 997
AleI CACNNNNGTG 1 cut(s) 714
AluBI AGCT 7 cut(s) 81, 124, 254, 852, 1198, 1244, 1302
AluI AGCT 7 cut(s) 81, 124, 254, 852, 1198, 1244, 1302
Alw21I GWGCWC 1 cut(s) 1107
Alw26I GTCTC 1 cut(s) 756
Alw44I GTGCAC 1 cut(s) 1103
AlwI GGATC 4 cut(s) 156, 169, 383, 812
Aor13HI TCCGGA 2 cut(s) 801, 984
AoxI GGCC 2 cut(s) 332, 823
ApaLI GTGCAC 1 cut(s) 1103
ApeKI GCWGC 2 cut(s) 121, 1051
ApoI RAATTY 2 cut(s) 577, 1142
ArsI GACNNNNNNTTYG 2 cut(s) 857, 889
Asp700I GAANNNNTTC 1 cut(s) 1191
AspLEI GCGC 2 cut(s) 94, 679
AspS9I GGNCC 4 cut(s) 172, 332, 824, 873
AsuC2I CCSGG 2 cut(s) 159, 828
AsuHPI GGTGA 5 cut(s) 361, 454, 943, 1155, 1213
AvaII GGWCC 2 cut(s) 172, 873
BaeGI GKGCMC 1 cut(s) 1107
BamHI GGATCC 1 cut(s) 161
BanII GRGCYC 1 cut(s) 505
BauI CACGAG 1 cut(s) 1288
Bbv12I GWGCWC 1 cut(s) 1107
BbvI GCAGC 2 cut(s) 133, 1038
BccI CCATC 5 cut(s) 200, 321, 695, 991, 1213
BceAI ACGGC 1 cut(s) 567
BcgI CGANNNNNNTGC 2 cut(s) 176, 210
BciT130I CCWGG 2 cut(s) 139, 1048
BciVI GTATCC 1 cut(s) 736
BcnI CCSGG 2 cut(s) 159, 828
BcoDI GTCTC 1 cut(s) 756
BfmI CTRYAG 1 cut(s) 777
BfoI RGCGCY 1 cut(s) 680
BfuI GTATCC 1 cut(s) 736
BisI GCNGC 3 cut(s) 122, 823, 1052
BlsI GCNGC 3 cut(s) 123, 824, 1053
Bme1390I CCNGG 4 cut(s) 139, 159, 828, 1048
Bme18I GGWCC 2 cut(s) 172, 873
BmgT120I GGNCC 4 cut(s) 172, 332, 824, 873
BmiI GGNNCC 4 cut(s) 163, 173, 826, 875
BmrFI CCNGG 4 cut(s) 139, 159, 828, 1048
BmrI ACTGGG 1 cut(s) 1378
BmsI GCATC 2 cut(s) 665, 714
BmuI ACTGGG 1 cut(s) 1378
BpuEI CTTGAG 1 cut(s) 1230
BpuMI CCSGG 2 cut(s) 159, 828
Bsa29I ATCGAT 1 cut(s) 1026
BsaBI GATNNNNATC 1 cut(s) 1022
BsaHI GRCGYC 1 cut(s) 210
BsaJI CCNNGG 3 cut(s) 137, 436, 826
BsaWI WCCGGW 2 cut(s) 801, 984
BsaXI ACNNNNNCTCC 2 cut(s) 195, 225
Bsc4I CCNNNNNNNGG 4 cut(s) 462, 833, 1172, 1211
Bse1I ACTGG 1 cut(s) 1384
Bse3DI GCAATG 2 cut(s) 375, 975
Bse8I GATNNNNATC 1 cut(s) 1022
BseAI TCCGGA 2 cut(s) 801, 984
BseBI CCWGG 2 cut(s) 139, 1048
BseCI ATCGAT 1 cut(s) 1026
BseDI CCNNGG 3 cut(s) 137, 436, 826
BseGI GGATG 3 cut(s) 313, 705, 1205
BseJI GATNNNNATC 1 cut(s) 1022
BseLI CCNNNNNNNGG 4 cut(s) 462, 833, 1172, 1211
BseMI GCAATG 2 cut(s) 375, 975
BseMII CTCAG 2 cut(s) 239, 679
BseNI ACTGG 1 cut(s) 1384
BseRI GAGGAG 1 cut(s) 116
BseSI GKGCMC 1 cut(s) 1107
BseXI GCAGC 2 cut(s) 133, 1038
BseYI CCCAGC 1 cut(s) 329
Bsh1236I CGCG 1 cut(s) 92
BshFI GGCC 2 cut(s) 334, 825
BshVI ATCGAT 1 cut(s) 1026
BsiHKAI GWGCWC 1 cut(s) 1107
BsiSI CCGG 4 cut(s) 159, 802, 828, 985
BslFI GGGAC 4 cut(s) 484, 859, 1129, 1304
BslI CCNNNNNNNGG 4 cut(s) 462, 833, 1172, 1211
BsmAI GTCTC 1 cut(s) 756
BsmBI CGTCTC 1 cut(s) 756
BsmFI GGGAC 4 cut(s) 484, 859, 1129, 1304
BsmI GAATGC 3 cut(s) 629, 905, 980
BsnI GGCC 2 cut(s) 334, 825
Bsp1286I GDGCHC 2 cut(s) 505, 1107
Bsp13I TCCGGA 2 cut(s) 801, 984
Bsp143I GATC 8 cut(s) 14, 161, 341, 360, 388, 804, 1027, 1218
Bsp19I CCATGG 1 cut(s) 436
BspACI CCGC 8 cut(s) 90, 271, 646, 822, 954, 1076, 1274, 1278
BspANI GGCC 2 cut(s) 334, 825
BspCNI CTCAG 2 cut(s) 238, 678
BspDI ATCGAT 1 cut(s) 1026
BspEI TCCGGA 2 cut(s) 801, 984
BspFNI CGCG 1 cut(s) 92
BspLI GGNNCC 4 cut(s) 163, 173, 826, 875
BspPI GGATC 4 cut(s) 156, 169, 383, 812
BsrDI GCAATG 2 cut(s) 375, 975
BsrI ACTGG 1 cut(s) 1384
BssECI CCNNGG 3 cut(s) 137, 436, 826
BssMI GATC 8 cut(s) 14, 161, 341, 360, 388, 804, 1027, 1218
BssNI GRCGYC 1 cut(s) 210
BssSI CACGAG 1 cut(s) 1288
BssT1I CCWWGG 1 cut(s) 436
Bst2BI CACGAG 1 cut(s) 1288
Bst2UI CCWGG 2 cut(s) 139, 1048
Bst4CI ACNGT 3 cut(s) 373, 457, 770
BstACI GRCGYC 1 cut(s) 210
BstC8I GCNNGC 7 cut(s) 79, 644, 681, 820, 1242, 1278, 1282
BstDEI CTNAG 2 cut(s) 225, 665
BstDSI CCRYGG 1 cut(s) 436
BstF5I GGATG 3 cut(s) 313, 705, 1205
BstFNI CGCG 1 cut(s) 92
BstH2I RGCGCY 1 cut(s) 680
BstHHI GCGC 2 cut(s) 94, 679
BstKTI GATC 8 cut(s) 17, 164, 344, 363, 391, 807, 1030, 1221
BstMAI GTCTC 1 cut(s) 756
BstMBI GATC 8 cut(s) 14, 161, 341, 360, 388, 804, 1027, 1218
BstMWI GCNNNNNNNGC 2 cut(s) 551, 849
BstNI CCWGG 2 cut(s) 139, 1048
BstNSI RCATGY 2 cut(s) 545, 646
BstSCI CCNGG 4 cut(s) 137, 157, 826, 1046
BstSFI CTRYAG 1 cut(s) 777
BstSLI GKGCMC 1 cut(s) 1107
BstUI CGCG 1 cut(s) 92
BstV1I GCAGC 2 cut(s) 133, 1038
BstX2I RGATCY 2 cut(s) 161, 388
BstYI RGATCY 2 cut(s) 161, 388
Bsu15I ATCGAT 1 cut(s) 1026
BsuI GTATCC 1 cut(s) 736
BsuRI GGCC 2 cut(s) 334, 825
BsuTUI ATCGAT 1 cut(s) 1026
BtgI CCRYGG 1 cut(s) 436
BtsCI GGATG 3 cut(s) 313, 705, 1205
BtsI GCAGTG 1 cut(s) 1159
BtsIMutI CAGTG 2 cut(s) 213, 1159
Cac8I GCNNGC 7 cut(s) 79, 644, 681, 820, 1242, 1278, 1282
CfoI GCGC 2 cut(s) 94, 679
Cfr13I GGNCC 4 cut(s) 172, 332, 824, 873
ClaI ATCGAT 1 cut(s) 1026
CseI GACGC 2 cut(s) 199, 708
Csp6I GTAC 1 cut(s) 369
CviQI GTAC 1 cut(s) 369
DdeI CTNAG 2 cut(s) 225, 665
DpnI GATC 8 cut(s) 16, 163, 343, 362, 390, 806, 1029, 1220
DpnII GATC 8 cut(s) 14, 161, 341, 360, 388, 804, 1027, 1218
DraI TTTAAA 1 cut(s) 235
DraIII CACNNNGTG 1 cut(s) 1169
Eco130I CCWWGG 1 cut(s) 436
Eco24I GRGCYC 1 cut(s) 505
Eco32I GATATC 1 cut(s) 325
Eco47I GGWCC 2 cut(s) 172, 873
Eco57I CTGAAG 1 cut(s) 405
EcoO109I RGGNCCY 1 cut(s) 172
EcoRII CCWGG 2 cut(s) 137, 1046
EcoRV GATATC 1 cut(s) 325
EcoT14I CCWWGG 1 cut(s) 436
EcoT38I GRGCYC 1 cut(s) 505
ErhI CCWWGG 1 cut(s) 436
Esp3I CGTCTC 1 cut(s) 756
FaqI GGGAC 4 cut(s) 484, 859, 1129, 1304
FauI CCCGC 3 cut(s) 639, 1281, 1285
FauNDI CATATG 1 cut(s) 60
FblI GTMKAC 2 cut(s) 538, 1269
Fnu4HI GCNGC 3 cut(s) 122, 823, 1052
FokI GGATG 3 cut(s) 300, 692, 1192
FriOI GRGCYC 1 cut(s) 505
Fsp4HI GCNGC 3 cut(s) 122, 823, 1052
GlaI GCGC 2 cut(s) 93, 678
GluI GCNGC 3 cut(s) 122, 823, 1052
GsaI CCCAGC 1 cut(s) 333
HaeII RGCGCY 1 cut(s) 680
HaeIII GGCC 2 cut(s) 334, 825
HapII CCGG 4 cut(s) 159, 802, 828, 985
HgaI GACGC 2 cut(s) 199, 708
HhaI GCGC 2 cut(s) 94, 679
Hin1I GRCGYC 1 cut(s) 210
Hin6I GCGC 2 cut(s) 92, 677
HinP1I GCGC 2 cut(s) 92, 677
HincII GTYRAC 2 cut(s) 539, 614
HindII GTYRAC 2 cut(s) 539, 614
HindIII AAGCTT 2 cut(s) 850, 1300
HpaII CCGG 4 cut(s) 159, 802, 828, 985
HphI GGTGA 5 cut(s) 361, 454, 943, 1155, 1213
Hpy166II GTNNAC 6 cut(s) 144, 445, 539, 614, 1105, 1270
Hpy188I TCNGA 4 cut(s) 155, 668, 1017, 1068
Hpy188III TCNNGA 5 cut(s) 383, 392, 795, 802, 985
Hpy8I GTNNAC 6 cut(s) 144, 445, 539, 614, 1105, 1270
Hpy99I CGWCG 2 cut(s) 420, 724
HpyAV CCTTC 7 cut(s) 20, 115, 455, 577, 718, 1108, 1166
HpyCH4III ACNGT 3 cut(s) 373, 457, 770
HpyCH4IV ACGT 2 cut(s) 418, 560
HpyF10VI GCNNNNNNNGC 2 cut(s) 551, 849
HpyF3I CTNAG 2 cut(s) 225, 665
HpySE526I ACGT 2 cut(s) 418, 560
Hsp92I GRCGYC 1 cut(s) 210
HspAI GCGC 2 cut(s) 92, 677
Kpn2I TCCGGA 2 cut(s) 801, 984
Kzo9I GATC 8 cut(s) 14, 161, 341, 360, 388, 804, 1027, 1218
LmnI GCTCC 3 cut(s) 129, 385, 550
Lsp1109I GCAGC 2 cut(s) 133, 1038
LweI GCATC 2 cut(s) 665, 714
MaeII ACGT 2 cut(s) 418, 560
MaeIII GTNAC 5 cut(s) 211, 349, 556, 1040, 1154
MalI GATC 8 cut(s) 16, 163, 343, 362, 390, 806, 1029, 1220
MboI GATC 8 cut(s) 14, 161, 341, 360, 388, 804, 1027, 1218
MboII GAAGA 4 cut(s) 9, 398, 783, 1199
MflI RGATCY 2 cut(s) 161, 388
MhlI GDGCHC 2 cut(s) 505, 1107
MluCI AATT 5 cut(s) 42, 285, 577, 787, 1142
MlyI GAGTC 4 cut(s) 104, 215, 346, 1037
MmeI TCCRAC 4 cut(s) 178, 572, 939, 996
MnlI CCTC 8 cut(s) 137, 162, 185, 461, 727, 979, 1123, 1237
MroI TCCGGA 2 cut(s) 801, 984
MroXI GAANNNNTTC 1 cut(s) 1191
MseI TTAA 3 cut(s) 234, 284, 1110
MslI CAYNNNNRTG 1 cut(s) 714
MspI CCGG 4 cut(s) 159, 802, 828, 985
MspR9I CCNGG 4 cut(s) 139, 159, 828, 1048
Mva1269I GAATGC 3 cut(s) 629, 905, 980
MvaI CCWGG 2 cut(s) 139, 1048
MvnI CGCG 1 cut(s) 92
MwoI GCNNNNNNNGC 2 cut(s) 551, 849
NciI CCSGG 2 cut(s) 159, 828
NcoI CCATGG 1 cut(s) 436
NdeI CATATG 1 cut(s) 60
NdeII GATC 8 cut(s) 14, 161, 341, 360, 388, 804, 1027, 1218
NlaIV GGNNCC 4 cut(s) 163, 173, 826, 875
NmuCI GTSAC 4 cut(s) 211, 349, 1040, 1154
NspI RCATGY 2 cut(s) 545, 646
OliI CACNNNNGTG 1 cut(s) 714
PaeI GCATGC 1 cut(s) 646
PcsI WCGNNNNNNNCGW 1 cut(s) 557
PctI GAATGC 3 cut(s) 629, 905, 980
PdmI GAANNNNTTC 1 cut(s) 1191
PfeI GAWTC 6 cut(s) 150, 279, 412, 592, 798, 936
PflFI GACNNNGTC 1 cut(s) 113
PflMI CCANNNNNTGG 1 cut(s) 1211
PkrI GCNGC 3 cut(s) 123, 824, 1053
PleI GAGTC 4 cut(s) 104, 215, 346, 1037
PpsI GAGTC 4 cut(s) 104, 215, 346, 1037
PpuMI RGGWCCY 1 cut(s) 172
Psp5II RGGWCCY 1 cut(s) 172
Psp6I CCWGG 2 cut(s) 137, 1046
PspFI CCCAGC 1 cut(s) 329
PspGI CCWGG 2 cut(s) 137, 1046
PspN4I GGNNCC 4 cut(s) 163, 173, 826, 875
PspPI GGNCC 4 cut(s) 172, 332, 824, 873
PspPPI RGGWCCY 1 cut(s) 172
PsuI RGATCY 2 cut(s) 161, 388
PsyI GACNNNGTC 1 cut(s) 113
RsaI GTAC 1 cut(s) 370
RsaNI GTAC 1 cut(s) 369
RseI CAYNNNNRTG 1 cut(s) 714
SalI GTCGAC 1 cut(s) 537
SaqAI TTAA 3 cut(s) 234, 284, 1110
SatI GCNGC 3 cut(s) 122, 823, 1052
Sau3AI GATC 8 cut(s) 14, 161, 341, 360, 388, 804, 1027, 1218
Sau96I GGNCC 4 cut(s) 172, 332, 824, 873
SchI GAGTC 4 cut(s) 104, 215, 346, 1037
ScrFI CCNGG 4 cut(s) 139, 159, 828, 1048
SduI GDGCHC 2 cut(s) 505, 1107
SfaNI GCATC 2 cut(s) 665, 714
SfcI CTRYAG 1 cut(s) 777
SinI GGWCC 2 cut(s) 172, 873
SmiMI CAYNNNNRTG 1 cut(s) 714
SmlI CTYRAG 1 cut(s) 1245
SmoI CTYRAG 1 cut(s) 1245
SphI GCATGC 1 cut(s) 646
Sse9I AATT 5 cut(s) 42, 285, 577, 787, 1142
SsiI CCGC 8 cut(s) 90, 271, 646, 822, 954, 1076, 1274, 1278
SspI AATATT 1 cut(s) 814
StyD4I CCNGG 4 cut(s) 137, 157, 826, 1046
StyI CCWWGG 1 cut(s) 436
TaaI ACNGT 3 cut(s) 373, 457, 770
TaiI ACGT 2 cut(s) 421, 563
TaqI TCGA 8 cut(s) 13, 108, 340, 415, 538, 722, 732, 1026
TaqII GACCGA 1 cut(s) 495
TasI AATT 5 cut(s) 42, 285, 577, 787, 1142
TauI GCSGC 1 cut(s) 825
TfiI GAWTC 6 cut(s) 150, 279, 412, 592, 798, 936
Tru1I TTAA 3 cut(s) 234, 284, 1110
Tru9I TTAA 3 cut(s) 234, 284, 1110
TscAI CASTG 2 cut(s) 220, 1159
TseFI GTSAC 4 cut(s) 211, 349, 1040, 1154
TseI GCWGC 2 cut(s) 121, 1051
Tsp45I GTSAC 4 cut(s) 211, 349, 1040, 1154
TspDTI ATGAA 4 cut(s) 47, 554, 1294, 1328
TspGWI ACGGA 3 cut(s) 103, 1142, 1198
TspRI CASTG 2 cut(s) 220, 1159
Tth111I GACNNNGTC 1 cut(s) 113
Van91I CCANNNNNTGG 1 cut(s) 1211
VneI GTGCAC 1 cut(s) 1103
VpaK11BI GGWCC 2 cut(s) 172, 873
XapI RAATTY 2 cut(s) 577, 1142
XceI RCATGY 2 cut(s) 545, 646
XcmI CCANNNNNNNNNTGG 1 cut(s) 138
XmiI GTMKAC 2 cut(s) 538, 1269
XmnI GAANNNNTTC 1 cut(s) 1191
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.