pycom17g21060

GDP-fucose protein O-fucosyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0008507
Physical Location & Seq
Forward (+)
19480320 .. 19484056
3737 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1314 bp
ATGGTGGCGATTTCAGTGAAGCCCGCCTTCATCGTAGGAGTCCTCACACTCTCTCTCGTCCTCGCCGTCCTCCTCTTATCGTCCCCCACTTCCCCTTTCTACAGAACCCCTCTCTTTCTCAATTCACCCATGAGCAAATTGGATATATGGAGTGTTGGGAGGATGGTAGAATGGAGACCTTGCAAGTGGTGGCTACAAGGACACCTCACTGCTCTGCCATCAAAGAGTAATGGATTCATTCGAGTGGATTGCTATGGGGGCCTTAATCAGATGAGGAGAGATTTATGTGATGGTGTTGGTATTGCCCGTCTTTTAAATGCAACCCTGGTTCTGCCAAAGTTTGAGGTGGCTGCTTATTGGAACGAATCTAGTGGTTTTGCAGATGTCTTTGATGTCGATTACTTCATTCAACAGTTGAATGGATTCATCAAAGTTGTCAAAGAGCTGCCATCAGAGATTTCATCAAAAGAACCTGTCCATGTAGATTGTAGCAAACGAAAGGGCCAATTTGATTACATTGAAAGTGTTCTTCCAGCCCTGTTAGAGCATCATTATATTTCAATTACACCAGCAATGAGCCAAAGACGGGATAGGTATCCTCAGTATGCAAAAGCTTCACTTTGTCAAGCTTGCTACAGTGCATTACGCCTCACTAAATCCTTGGAGAAGAAGGCCTCCGAGCTTCTTCAAGCCATACCCAAACCTTTCCTCTCACTTCACCTTCGTTTTGAGCCTGATATGGTAGCATACAGCCAATGTGAATATCCATACCTTTCTCCTTCCTCCATTGAAGCCATTGAGGCAGCGCGAGGAGATAGAAAACCATGGACCGGAGAGCTTGCCCAGATTTGGAGGAAACGAGGGAAATGTCCTCTTACGCCTAATGAGACAGCCTTCATACTCCAAGCGCTATCCATCCCAACGAACACAAATATTTATCTGGCAGCTGGGGACGGTCTAATGGAAATTGAAGGGTTAACTTCTGTTTACACGAATGTAGTTACCAAGTCTAGCCTTCTTAGCGGTGAGGATTTCAAAAGCATGCATGGGAACACGAAAGCAGCGTTGGACTACTACGTGTCGATTAATAGCGATTCATATATCGCAACATATTTTGGAAATATGGATAAGATGGTTGCAGCAATGCGAGCTTTTAAGGGATTGTACAAGACACTTTTCTTAAGCAGAAGAGCTTTTGCAGAGTTCACTTCTCAAGGTTTGAGCGGGAAGGAATTGATGGAAGCTCTATGGAAGGCCCACAGAGATGATTTTGTCATGGGGAAAGGATCGAGCTTTGCCGGATTGCTTTTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
O-FucT

Protein Analysis

438

Amino Acids

48.89

Weight (kDa)

8.82

Isoelectric Point (pI)

40.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
O-FucT PF10250 79 - 385 4.5e-64 GDP-fucose protein O-fucosyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 1224
AccII CGCG 1 cut(s) 808
AciI CCGC 3 cut(s) 24, 1023, 1224
AclWI GGATC 1 cut(s) 1294
AfaI GTAC 1 cut(s) 1166
AfeI AGCGCT 1 cut(s) 909
AfiI CCNNNNNNNGG 3 cut(s) 586, 830, 849
AflII CTTAAG 1 cut(s) 1180
AflIII ACRYGT 1 cut(s) 1077
AgsI TTSAA 8 cut(s) 410, 418, 521, 561, 689, 791, 971, 1036
AjnI CCWGG 1 cut(s) 324
AjuI GAANNNNNNNTTGG 2 cut(s) 1049, 1081
Alw26I GTCTC 2 cut(s) 169, 881
AlwI GGATC 1 cut(s) 1294
Aor51HI AGCGCT 1 cut(s) 909
AoxI GGCC 4 cut(s) 259, 502, 672, 1254
ApeKI GCWGC 6 cut(s) 350, 445, 803, 944, 1061, 1139
AseI ATTAAT 1 cut(s) 1086
Asp700I GAANNNNTTC 2 cut(s) 422, 525
AspLEI GCGC 2 cut(s) 808, 910
AspS9I GGNCC 4 cut(s) 259, 502, 828, 1255
AsuHPI GGTGA 3 cut(s) 117, 710, 1037
AvaII GGWCC 1 cut(s) 828
BbvI GCAGC 6 cut(s) 337, 432, 815, 956, 1073, 1151
BccI CCATC 7 cut(s) 157, 226, 284, 457, 923, 1126, 1231
BceAI ACGGC 1 cut(s) 50
BcgI CGANNNNNNTGC 2 cut(s) 231, 265
BciT130I CCWGG 1 cut(s) 326
BciVI GTATCC 1 cut(s) 606
BcoDI GTCTC 2 cut(s) 169, 881
BfaI CTAG 2 cut(s) 369, 1011
BfmI CTRYAG 2 cut(s) 100, 634
BfoI RGCGCY 1 cut(s) 911
BfrI CTTAAG 1 cut(s) 1180
BfuI GTATCC 1 cut(s) 606
BglI GCCNNNNNGGC 1 cut(s) 800
BisI GCNGC 6 cut(s) 351, 446, 804, 945, 1062, 1140
BlsI GCNGC 6 cut(s) 352, 447, 805, 946, 1063, 1141
Bme1390I CCNGG 1 cut(s) 326
Bme18I GGWCC 1 cut(s) 828
BmgT120I GGNCC 4 cut(s) 259, 502, 828, 1255
BmiI GGNNCC 1 cut(s) 260
BmrFI CCNGG 1 cut(s) 326
BmsI GCATC 1 cut(s) 556
BpuEI CTTGAG 1 cut(s) 1197
BsaAI YACGTR 1 cut(s) 1078
BsaBI GATNNNNATC 1 cut(s) 594
BsaI GGTCTC 1 cut(s) 169
BsaJI CCNNGG 3 cut(s) 324, 660, 824
BsaWI WCCGGW 1 cut(s) 830
Bsc4I CCNNNNNNNGG 3 cut(s) 586, 830, 849
Bse3DI GCAATG 2 cut(s) 579, 1149
Bse8I GATNNNNATC 1 cut(s) 594
BseBI CCWGG 1 cut(s) 326
BseDI CCNNGG 3 cut(s) 324, 660, 824
BseGI GGATG 2 cut(s) 168, 915
BseJI GATNNNNATC 1 cut(s) 594
BseLI CCNNNNNNNGG 3 cut(s) 586, 830, 849
BseMI GCAATG 2 cut(s) 579, 1149
BseMII CTCAG 1 cut(s) 614
BseRI GAGGAG 3 cut(s) 62, 289, 825
BseXI GCAGC 6 cut(s) 337, 432, 815, 956, 1073, 1151
BseYI CCCAGC 1 cut(s) 947
Bsh1236I CGCG 1 cut(s) 808
BshFI GGCC 4 cut(s) 261, 504, 674, 1256
BsiSI CCGG 2 cut(s) 831, 1299
BslFI GGGAC 2 cut(s) 67, 965
BslI CCNNNNNNNGG 3 cut(s) 586, 830, 849
BsmAI GTCTC 2 cut(s) 169, 881
BsmFI GGGAC 2 cut(s) 67, 965
BsnI GGCC 4 cut(s) 261, 504, 674, 1256
Bso31I GGTCTC 1 cut(s) 169
Bsp1407I TGTACA 1 cut(s) 1164
Bsp143I GATC 1 cut(s) 1286
Bsp19I CCATGG 1 cut(s) 824
BspACI CCGC 3 cut(s) 24, 1023, 1224
BspANI GGCC 4 cut(s) 261, 504, 674, 1256
BspCNI CTCAG 1 cut(s) 613
BspFNI CGCG 1 cut(s) 808
BspLI GGNNCC 1 cut(s) 260
BspPI GGATC 1 cut(s) 1294
BspQI GCTCTTC 1 cut(s) 1183
BspTI CTTAAG 1 cut(s) 1180
BspTNI GGTCTC 1 cut(s) 169
BsrBI CCGCTC 1 cut(s) 1224
BsrDI GCAATG 2 cut(s) 579, 1149
BsrGI TGTACA 1 cut(s) 1164
BssECI CCNNGG 3 cut(s) 324, 660, 824
BssMI GATC 1 cut(s) 1286
BssT1I CCWWGG 2 cut(s) 660, 824
Bst2UI CCWGG 1 cut(s) 326
Bst4CI ACNGT 3 cut(s) 414, 638, 956
Bst6I CTCTTC 1 cut(s) 1183
BstAFI CTTAAG 1 cut(s) 1180
BstAUI TGTACA 1 cut(s) 1164
BstBAI YACGTR 1 cut(s) 1078
BstC8I GCNNGC 5 cut(s) 24, 631, 840, 1043, 1149
BstDEI CTNAG 2 cut(s) 600, 1019
BstDSI CCRYGG 1 cut(s) 824
BstF5I GGATG 2 cut(s) 168, 915
BstFNI CGCG 1 cut(s) 808
BstH2I RGCGCY 1 cut(s) 911
BstHHI GCGC 2 cut(s) 808, 910
BstKTI GATC 1 cut(s) 1289
BstMAI GTCTC 2 cut(s) 169, 881
BstMBI GATC 1 cut(s) 1286
BstMWI GCNNNNNNNGC 4 cut(s) 258, 800, 1020, 1148
BstNI CCWGG 1 cut(s) 326
BstNSI RCATGY 1 cut(s) 1045
BstSCI CCNGG 1 cut(s) 324
BstSFI CTRYAG 2 cut(s) 100, 634
BstUI CGCG 1 cut(s) 808
BstV1I GCAGC 6 cut(s) 337, 432, 815, 956, 1073, 1151
BsuI GTATCC 1 cut(s) 606
BsuRI GGCC 4 cut(s) 261, 504, 674, 1256
BtgI CCRYGG 1 cut(s) 824
BtsCI GGATG 2 cut(s) 168, 915
BtsI GCAGTG 1 cut(s) 207
BtsIMutI CAGTG 3 cut(s) 21, 207, 643
Cac8I GCNNGC 5 cut(s) 24, 631, 840, 1043, 1149
CfoI GCGC 2 cut(s) 808, 910
Cfr13I GGNCC 4 cut(s) 259, 502, 828, 1255
Csp6I GTAC 1 cut(s) 1165
CviAII CATG 6 cut(s) 130, 479, 825, 1042, 1046, 1276
CviQI GTAC 1 cut(s) 1165
DdeI CTNAG 2 cut(s) 600, 1019
DpnI GATC 1 cut(s) 1288
DpnII GATC 1 cut(s) 1286
DraI TTTAAA 1 cut(s) 315
Eam1104I CTCTTC 1 cut(s) 1183
EarI CTCTTC 1 cut(s) 1183
Eco130I CCWWGG 2 cut(s) 660, 824
Eco147I AGGCCT 1 cut(s) 674
Eco31I GGTCTC 1 cut(s) 169
Eco47I GGWCC 1 cut(s) 828
Eco47III AGCGCT 1 cut(s) 909
EcoO109I RGGNCCY 1 cut(s) 259
EcoRII CCWGG 1 cut(s) 324
EcoT14I CCWWGG 2 cut(s) 660, 824
EcoT22I ATGCAT 1 cut(s) 1047
ErhI CCWWGG 2 cut(s) 660, 824
FaeI CATG 6 cut(s) 133, 482, 828, 1045, 1049, 1279
FalI AAGNNNNNCTT 4 cut(s) 11, 43, 603, 635
FaqI GGGAC 2 cut(s) 67, 965
FatI CATG 6 cut(s) 129, 478, 824, 1041, 1045, 1275
FauI CCCGC 2 cut(s) 31, 1217
Fnu4HI GCNGC 6 cut(s) 351, 446, 804, 945, 1062, 1140
FokI GGATG 2 cut(s) 175, 902
Fsp4HI GCNGC 6 cut(s) 351, 446, 804, 945, 1062, 1140
FspBI CTAG 2 cut(s) 369, 1011
GlaI GCGC 2 cut(s) 807, 909
GluI GCNGC 6 cut(s) 351, 446, 804, 945, 1062, 1140
GsaI CCCAGC 1 cut(s) 951
HaeII RGCGCY 1 cut(s) 911
HaeIII GGCC 4 cut(s) 261, 504, 674, 1256
HapII CCGG 2 cut(s) 831, 1299
HhaI GCGC 2 cut(s) 808, 910
Hin1II CATG 6 cut(s) 133, 482, 828, 1045, 1049, 1279
Hin6I GCGC 2 cut(s) 806, 908
HinP1I GCGC 2 cut(s) 806, 908
HincII GTYRAC 1 cut(s) 978
HindII GTYRAC 1 cut(s) 978
HindIII AAGCTT 2 cut(s) 612, 627
HinfI GANTC 5 cut(s) 39, 234, 365, 423, 1094
HpaI GTTAAC 1 cut(s) 978
HpaII CCGG 2 cut(s) 831, 1299
HphI GGTGA 3 cut(s) 117, 710, 1037
Hpy166II GTNNAC 3 cut(s) 978, 988, 1206
Hpy188I TCNGA 3 cut(s) 270, 454, 679
Hpy8I GTNNAC 3 cut(s) 978, 988, 1206
HpyAV CCTTC 9 cut(s) 37, 664, 731, 789, 904, 965, 1025, 1222, 1246
HpyCH4III ACNGT 3 cut(s) 414, 638, 956
HpyCH4IV ACGT 1 cut(s) 1077
HpyCH4V TGCA 8 cut(s) 183, 320, 380, 608, 641, 1045, 1139, 1199
HpyF10VI GCNNNNNNNGC 4 cut(s) 258, 800, 1020, 1148
HpyF3I CTNAG 2 cut(s) 600, 1019
HpySE526I ACGT 1 cut(s) 1077
Hsp92II CATG 6 cut(s) 133, 482, 828, 1045, 1049, 1279
HspAI GCGC 2 cut(s) 806, 908
KspAI GTTAAC 1 cut(s) 978
Kzo9I GATC 1 cut(s) 1286
LguI GCTCTTC 1 cut(s) 1183
Lsp1109I GCAGC 6 cut(s) 337, 432, 815, 956, 1073, 1151
LweI GCATC 1 cut(s) 556
MaeI CTAG 2 cut(s) 369, 1011
MaeII ACGT 1 cut(s) 1077
MaeIII GTNAC 1 cut(s) 1000
MalI GATC 1 cut(s) 1288
MbiI CCGCTC 1 cut(s) 1224
MboI GATC 1 cut(s) 1286
MboII GAAGA 4 cut(s) 521, 677, 679, 1200
MluCI AATT 6 cut(s) 121, 137, 506, 561, 966, 1232
MlyI GAGTC 1 cut(s) 48
MmeI TCCRAC 1 cut(s) 1047
Mph1103I ATGCAT 1 cut(s) 1047
MroXI GAANNNNTTC 2 cut(s) 422, 525
MseI TTAA 6 cut(s) 264, 314, 977, 1086, 1155, 1181
MslI CAYNNNNRTG 2 cut(s) 242, 1263
MspA1I CMGCKG 1 cut(s) 947
MspCI CTTAAG 1 cut(s) 1180
MspI CCGG 2 cut(s) 831, 1299
MspR9I CCNGG 1 cut(s) 326
MvaI CCWGG 1 cut(s) 326
MvnI CGCG 1 cut(s) 808
MwoI GCNNNNNNNGC 4 cut(s) 258, 800, 1020, 1148
NcoI CCATGG 1 cut(s) 824
NdeII GATC 1 cut(s) 1286
NlaIII CATG 6 cut(s) 133, 482, 828, 1045, 1049, 1279
NlaIV GGNNCC 1 cut(s) 260
NsiI ATGCAT 1 cut(s) 1047
NspI RCATGY 1 cut(s) 1045
PaeI GCATGC 1 cut(s) 1045
PceI AGGCCT 1 cut(s) 674
PciSI GCTCTTC 1 cut(s) 1183
PcsI WCGNNNNNNNCGW 2 cut(s) 63, 1061
PdmI GAANNNNTTC 2 cut(s) 422, 525
PfeI GAWTC 4 cut(s) 234, 365, 423, 1094
PkrI GCNGC 6 cut(s) 352, 447, 805, 946, 1063, 1141
PleI GAGTC 1 cut(s) 47
PpsI GAGTC 1 cut(s) 47
Ppu21I YACGTR 1 cut(s) 1078
PshBI ATTAAT 1 cut(s) 1086
Psp6I CCWGG 1 cut(s) 324
PspFI CCCAGC 1 cut(s) 947
PspGI CCWGG 1 cut(s) 324
PspN4I GGNNCC 1 cut(s) 260
PspPI GGNCC 4 cut(s) 259, 502, 828, 1255
PvuII CAGCTG 1 cut(s) 947
RsaI GTAC 1 cut(s) 1166
RsaNI GTAC 1 cut(s) 1165
RseI CAYNNNNRTG 2 cut(s) 242, 1263
SapI GCTCTTC 1 cut(s) 1183
SaqAI TTAA 6 cut(s) 264, 314, 977, 1086, 1155, 1181
SatI GCNGC 6 cut(s) 351, 446, 804, 945, 1062, 1140
Sau3AI GATC 1 cut(s) 1286
Sau96I GGNCC 4 cut(s) 259, 502, 828, 1255
SchI GAGTC 1 cut(s) 48
ScrFI CCNGG 1 cut(s) 326
SfaNI GCATC 1 cut(s) 556
SfcI CTRYAG 2 cut(s) 100, 634
SinI GGWCC 1 cut(s) 828
SmiMI CAYNNNNRTG 2 cut(s) 242, 1263
SmlI CTYRAG 2 cut(s) 1180, 1212
SmoI CTYRAG 2 cut(s) 1180, 1212
SphI GCATGC 1 cut(s) 1045
Sse9I AATT 6 cut(s) 121, 137, 506, 561, 966, 1232
SseBI AGGCCT 1 cut(s) 674
SsiI CCGC 3 cut(s) 24, 1023, 1224
SspI AATATT 1 cut(s) 934
SspMI CTAG 2 cut(s) 369, 1011
StuI AGGCCT 1 cut(s) 674
StyD4I CCNGG 1 cut(s) 324
StyI CCWWGG 2 cut(s) 660, 824
TaaI ACNGT 3 cut(s) 414, 638, 956
TaiI ACGT 1 cut(s) 1080
TaqI TCGA 4 cut(s) 241, 396, 1082, 1289
TasI AATT 6 cut(s) 121, 137, 506, 561, 966, 1232
TatI WGTACW 1 cut(s) 1164
TfiI GAWTC 4 cut(s) 234, 365, 423, 1094
Tru1I TTAA 6 cut(s) 264, 314, 977, 1086, 1155, 1181
Tru9I TTAA 6 cut(s) 264, 314, 977, 1086, 1155, 1181
TscAI CASTG 3 cut(s) 21, 214, 643
TseI GCWGC 6 cut(s) 350, 445, 803, 944, 1061, 1139
TspDTI ATGAA 7 cut(s) 19, 226, 394, 415, 450, 886, 1086
TspRI CASTG 3 cut(s) 21, 214, 643
Vha464I CTTAAG 1 cut(s) 1180
VpaK11BI GGWCC 1 cut(s) 828
VspI ATTAAT 1 cut(s) 1086
XceI RCATGY 1 cut(s) 1045
XcmI CCANNNNNNNNNTGG 1 cut(s) 136
XmnI GAANNNNTTC 2 cut(s) 422, 525
XspI CTAG 2 cut(s) 369, 1011
Zsp2I ATGCAT 1 cut(s) 1047
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.