pycom17g24370

UBA-like domain (DUF1421)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0010117
Physical Location & Seq
Reverse (-)
22697457 .. 22699599
2143 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1254 bp
ATGAAGAAGCAGAGTCAAAGCTCAAGTCTCTTGAGAAACATCTTCAAGAAACGATGCTTTGGTTTACTTGCACACTCGATCATAAGGGACAAGCAAGAACTTGCGGAGGCTCAGAAAGAACTAGCTAAGCTTCAGCTTGCACAGAAAGGATCTGCATCTTCAACCCATTCCCAATCCAATGAGGACAGAGCTTCACCACCTACCTCTGATGCTCAGAAAGCTGACCACCCATCCGAAACACATAACCAGCAATTGGCTTTAGCCCTGCCACATCAAGTGGCGCCACAGCAGCAGCCTGTGGCACCTCCCACACAGCCCCCTCCGCAGAATGTGACTCCACAACAATCCTATTATTTACCAACCCAGTTACAAAACCCACCACCTCAGGCACAACATCCGCAGAATCAATATTTGCCTCCTGATTCTCAGTATCGAAACCCCCAAGTGCAAGAAATGCCAAGGGTGACACCACGGCCAACACAATCGCAAGTGAATCAGGCACAAGTCCAGTCGTTCCCTCCGTATTCGCAACAATTACCGCAGAGTCAGGTGCAATTTCCACAACAGTCCTCTATGCAACCCCAGATGAGGCCACCATCAACAAATGTCTACCCTTATCCACCTAGTCAACAAACAAACCCATCTCCTCCAGAGTCTGTACCAAACAGCATGCCGATGCCAGTGCAATATTCCAACATTCCTCAACCAGGGCCGGGCCGTTCTGATCCCATGCCTTTTGGATATGGTGGAGCTGGTCGAACAATGCAGCAGCAGCCTCCGCCTCAGCAAATCAAGGGGGCTTTTGGTGCTCAACCAGGTGAGGGATATGGGGCTGCTGGTCCTCACACTGCACTTCCTCCAGGGAGTACTTATATGGTCTACGACAGTGAAGGGGGTAGAACTCAATATTCTGCTCATTATGGATATCCTCCTACAAGCATCTCTCATCATACACCACAGCCAACAACAGCTCCAAATCTTATGGCTCGGAATCAACAATTCCCACCTAACCACCCTTACAATGAGTTGATTGAGAAATTGGTACCCATGGGGTTCAGGAGCGATCACGTTGCGAGCGTGATTCAGAGGATGGAGGAAAGTGGCCAACCCATTGATTTTAATGCAGTGATTGACAGGTTGAATGCACATCCATCTGGGGTCCCTCAGAGAGGATGGTCAGGCGATGAAGGCCCCGGTGCGGTGCATTTTGTTGATGTTTTCATGAGGCTCAACTTTGAGATTACGTTGTTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1421

Protein Analysis

418

Amino Acids

45.98

Weight (kDa)

7.9

Isoelectric Point (pI)

70.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1421 PF07223 340 - 383 3.1e-22 UBA-like domain (DUF1421)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 1042
AccB1I GGYRCC 3 cut(s) 280, 301, 1042
AccB7I CCANNNNNTGG 1 cut(s) 253
AccI GTMKAC 2 cut(s) 609, 879
AciI CCGC 6 cut(s) 104, 323, 398, 539, 779, 1199
AclWI GGATC 2 cut(s) 157, 719
AcoI YGGCCR 2 cut(s) 473, 1102
AcuI CTGAAG 1 cut(s) 116
AcyI GRCGYC 1 cut(s) 281
AfaI GTAC 3 cut(s) 660, 868, 1044
AfiI CCNNNNNNNGG 7 cut(s) 253, 588, 707, 713, 821, 1169, 1198
AgsI TTSAA 3 cut(s) 46, 162, 1141
AjnI CCWGG 3 cut(s) 706, 814, 859
AluBI AGCT 8 cut(s) 21, 125, 130, 136, 191, 221, 752, 971
AluI AGCT 8 cut(s) 21, 125, 130, 136, 191, 221, 752, 971
Alw21I GWGCWC 1 cut(s) 811
Alw26I GTCTC 1 cut(s) 32
AlwI GGATC 2 cut(s) 157, 719
AlwNI CAGNNNCTG 1 cut(s) 656
AoxI GGCC 6 cut(s) 473, 590, 710, 715, 1102, 1189
ApeKI GCWGC 6 cut(s) 289, 292, 766, 769, 772, 833
ArsI GACNNNNNNTTYG 2 cut(s) 10, 42
Asp718I GGTACC 1 cut(s) 1042
AspLEI GCGC 1 cut(s) 283
AspS9I GGNCC 5 cut(s) 710, 715, 839, 1159, 1190
AsuC2I CCSGG 2 cut(s) 714, 1194
AsuHPI GGTGA 3 cut(s) 186, 475, 830
AvaII GGWCC 2 cut(s) 839, 1159
AxyI CCTNAGG 1 cut(s) 384
BalI TGGCCA 1 cut(s) 1104
BanI GGYRCC 3 cut(s) 280, 301, 1042
Bbv12I GWGCWC 1 cut(s) 811
BbvCI CCTCAGC 1 cut(s) 783
BbvI GCAGC 6 cut(s) 301, 304, 778, 781, 784, 820
BccI CCATC 6 cut(s) 238, 604, 649, 1084, 1159, 1167
BceAI ACGGC 2 cut(s) 488, 702
BcgI CGANNNNNNTGC 4 cut(s) 664, 698, 1052, 1086
BciT130I CCWGG 3 cut(s) 708, 816, 861
BcnI CCSGG 2 cut(s) 714, 1194
BcoDI GTCTC 1 cut(s) 32
BfaI CTAG 2 cut(s) 122, 624
BfoI RGCGCY 1 cut(s) 284
BisI GCNGC 6 cut(s) 290, 293, 767, 770, 773, 834
BlpI GCTNAGC 1 cut(s) 126
BlsI GCNGC 6 cut(s) 291, 294, 768, 771, 774, 835
BmcAI AGTACT 1 cut(s) 868
Bme1390I CCNGG 5 cut(s) 708, 714, 816, 861, 1194
Bme18I GGWCC 2 cut(s) 839, 1159
BmgT120I GGNCC 5 cut(s) 710, 715, 839, 1159, 1190
BmiI GGNNCC 6 cut(s) 282, 303, 1044, 1160, 1161, 1192
BmrFI CCNGG 5 cut(s) 708, 714, 816, 861, 1194
BmrI ACTGGG 1 cut(s) 358
BmsI GCATC 5 cut(s) 44, 164, 199, 666, 948
BmuI ACTGGG 1 cut(s) 358
BpmI CTGGAG 2 cut(s) 633, 843
Bpu10I CCTNAGC 1 cut(s) 783
Bpu1102I GCTNAGC 1 cut(s) 126
BpuEI CTTGAG 2 cut(s) 7, 52
BpuMI CCSGG 2 cut(s) 714, 1194
BsaBI GATNNNNATC 1 cut(s) 154
BsaHI GRCGYC 1 cut(s) 281
BsaJI CCNNGG 6 cut(s) 458, 470, 707, 860, 1047, 1192
BsaXI ACNNNNNCTCC 2 cut(s) 955, 985
Bsc4I CCNNNNNNNGG 7 cut(s) 253, 588, 707, 713, 821, 1169, 1198
Bse1I ACTGG 3 cut(s) 364, 508, 680
Bse21I CCTNAGG 1 cut(s) 384
Bse8I GATNNNNATC 1 cut(s) 154
BseBI CCWGG 3 cut(s) 708, 816, 861
BseDI CCNNGG 6 cut(s) 458, 470, 707, 860, 1047, 1192
BseGI GGATG 5 cut(s) 230, 394, 1095, 1147, 1178
BseJI GATNNNNATC 1 cut(s) 154
BseLI CCNNNNNNNGG 7 cut(s) 253, 588, 707, 713, 821, 1169, 1198
BseMII CTCAG 6 cut(s) 125, 227, 398, 440, 797, 1178
BseNI ACTGG 3 cut(s) 364, 508, 680
BseRI GAGGAG 1 cut(s) 636
BseXI GCAGC 6 cut(s) 301, 304, 778, 781, 784, 820
BsgI GTGCAG 1 cut(s) 834
BshFI GGCC 6 cut(s) 475, 592, 712, 717, 1104, 1191
BshNI GGYRCC 3 cut(s) 280, 301, 1042
BsiHKAI GWGCWC 1 cut(s) 811
BsiSI CCGG 2 cut(s) 713, 1194
BslFI GGGAC 2 cut(s) 101, 1145
BslI CCNNNNNNNGG 7 cut(s) 253, 588, 707, 713, 821, 1169, 1198
BsmAI GTCTC 1 cut(s) 32
BsmFI GGGAC 2 cut(s) 101, 1145
BsmI GAATGC 1 cut(s) 1147
BsnI GGCC 6 cut(s) 475, 592, 712, 717, 1104, 1191
Bsp1286I GDGCHC 1 cut(s) 811
Bsp143I GATC 4 cut(s) 78, 149, 724, 1063
Bsp1720I GCTNAGC 1 cut(s) 126
Bsp19I CCATGG 1 cut(s) 1047
BspACI CCGC 6 cut(s) 104, 323, 398, 539, 779, 1199
BspANI GGCC 6 cut(s) 475, 592, 712, 717, 1104, 1191
BspCNI CTCAG 6 cut(s) 124, 226, 397, 439, 796, 1177
BspHI TCATGA 1 cut(s) 1221
BspLI GGNNCC 6 cut(s) 282, 303, 1044, 1160, 1161, 1192
BspPI GGATC 2 cut(s) 157, 719
BspT107I GGYRCC 3 cut(s) 280, 301, 1042
BsrI ACTGG 3 cut(s) 364, 508, 680
BssECI CCNNGG 6 cut(s) 458, 470, 707, 860, 1047, 1192
BssMI GATC 4 cut(s) 78, 149, 724, 1063
BssNI GRCGYC 1 cut(s) 281
BssT1I CCWWGG 2 cut(s) 458, 1047
Bst2UI CCWGG 3 cut(s) 708, 816, 861
Bst4CI ACNGT 2 cut(s) 567, 887
BstACI GRCGYC 1 cut(s) 281
BstAPI GCANNNNNTGC 1 cut(s) 454
BstC8I GCNNGC 3 cut(s) 138, 671, 1075
BstDEI CTNAG 7 cut(s) 111, 126, 213, 384, 426, 783, 1164
BstDSI CCRYGG 2 cut(s) 470, 1047
BstENI CCTNNNNNAGG 2 cut(s) 705, 1167
BstF5I GGATG 5 cut(s) 230, 394, 1095, 1147, 1178
BstH2I RGCGCY 1 cut(s) 284
BstHHI GCGC 1 cut(s) 283
BstKTI GATC 4 cut(s) 81, 152, 727, 1066
BstMAI GTCTC 1 cut(s) 32
BstMBI GATC 4 cut(s) 78, 149, 724, 1063
BstMWI GCNNNNNNNGC 8 cut(s) 218, 289, 322, 454, 772, 778, 806, 1188
BstNI CCWGG 3 cut(s) 708, 816, 861
BstNSI RCATGY 1 cut(s) 673
BstSCI CCNGG 5 cut(s) 706, 712, 814, 859, 1192
BstV1I GCAGC 6 cut(s) 301, 304, 778, 781, 784, 820
BstX2I RGATCY 1 cut(s) 149
BstYI RGATCY 1 cut(s) 149
Bsu36I CCTNAGG 1 cut(s) 384
BsuRI GGCC 6 cut(s) 475, 592, 712, 717, 1104, 1191
BtgI CCRYGG 2 cut(s) 470, 1047
BtgZI GCGATG 1 cut(s) 1197
BtsCI GGATG 5 cut(s) 230, 394, 1095, 1147, 1178
BtsI GCAGTG 2 cut(s) 846, 1131
BtsIMutI CAGTG 4 cut(s) 687, 846, 892, 1131
Cac8I GCNNGC 3 cut(s) 138, 671, 1075
CaiI CAGNNNCTG 1 cut(s) 656
CciI TCATGA 1 cut(s) 1221
CfoI GCGC 1 cut(s) 283
Cfr13I GGNCC 5 cut(s) 710, 715, 839, 1159, 1190
CsiI ACCWGGT 1 cut(s) 814
Csp6I GTAC 3 cut(s) 659, 867, 1043
CviAII CATG 4 cut(s) 670, 730, 1048, 1222
CviQI GTAC 3 cut(s) 659, 867, 1043
DdeI CTNAG 7 cut(s) 111, 126, 213, 384, 426, 783, 1164
DinI GGCGCC 1 cut(s) 282
DpnI GATC 4 cut(s) 80, 151, 726, 1065
DpnII GATC 4 cut(s) 78, 149, 724, 1063
EaeI YGGCCR 2 cut(s) 473, 1102
EciI GGCGGA 1 cut(s) 768
Eco130I CCWWGG 2 cut(s) 458, 1047
Eco32I GATATC 1 cut(s) 926
Eco47I GGWCC 2 cut(s) 839, 1159
Eco57I CTGAAG 1 cut(s) 116
Eco81I CCTNAGG 1 cut(s) 384
EcoNI CCTNNNNNAGG 2 cut(s) 705, 1167
EcoO109I RGGNCCY 2 cut(s) 1159, 1190
EcoRII CCWGG 3 cut(s) 706, 814, 859
EcoRV GATATC 1 cut(s) 926
EcoT14I CCWWGG 2 cut(s) 458, 1047
EgeI GGCGCC 1 cut(s) 282
EheI GGCGCC 1 cut(s) 282
ErhI CCWWGG 2 cut(s) 458, 1047
FaeI CATG 4 cut(s) 673, 733, 1051, 1225
FaqI GGGAC 2 cut(s) 101, 1145
FatI CATG 4 cut(s) 669, 729, 1047, 1221
FblI GTMKAC 2 cut(s) 609, 879
Fnu4HI GCNGC 6 cut(s) 290, 293, 767, 770, 773, 834
FokI GGATG 5 cut(s) 217, 381, 1102, 1134, 1185
Fsp4HI GCNGC 6 cut(s) 290, 293, 767, 770, 773, 834
FspBI CTAG 2 cut(s) 122, 624
GlaI GCGC 1 cut(s) 282
GluI GCNGC 6 cut(s) 290, 293, 767, 770, 773, 834
GsuI CTGGAG 2 cut(s) 633, 843
HaeII RGCGCY 1 cut(s) 284
HaeIII GGCC 6 cut(s) 475, 592, 712, 717, 1104, 1191
HapII CCGG 2 cut(s) 713, 1194
HhaI GCGC 1 cut(s) 283
Hin1I GRCGYC 1 cut(s) 281
Hin1II CATG 4 cut(s) 673, 733, 1051, 1225
Hin6I GCGC 1 cut(s) 281
HinP1I GCGC 1 cut(s) 281
HincII GTYRAC 1 cut(s) 629
HindII GTYRAC 1 cut(s) 629
HindIII AAGCTT 1 cut(s) 128
HinfI GANTC 9 cut(s) 13, 334, 403, 422, 493, 544, 653, 991, 1081
HpaII CCGG 2 cut(s) 713, 1194
HphI GGTGA 3 cut(s) 186, 475, 830
Hpy166II GTNNAC 4 cut(s) 65, 610, 629, 880
Hpy188I TCNGA 8 cut(s) 114, 208, 216, 235, 724, 990, 1086, 1167
Hpy188III TCNNGA 6 cut(s) 31, 46, 419, 650, 1057, 1222
Hpy8I GTNNAC 4 cut(s) 65, 610, 629, 880
HpyAV CCTTC 2 cut(s) 884, 1181
HpyCH4III ACNGT 2 cut(s) 567, 887
HpyCH4IV ACGT 2 cut(s) 1068, 1244
HpyF10VI GCNNNNNNNGC 8 cut(s) 218, 289, 322, 454, 772, 778, 806, 1188
HpyF3I CTNAG 7 cut(s) 111, 126, 213, 384, 426, 783, 1164
HpySE526I ACGT 2 cut(s) 1068, 1244
Hsp92I GRCGYC 1 cut(s) 281
Hsp92II CATG 4 cut(s) 673, 733, 1051, 1225
HspAI GCGC 1 cut(s) 281
KasI GGCGCC 1 cut(s) 280
KflI GGGWCCC 1 cut(s) 1159
KpnI GGTACC 1 cut(s) 1046
Kzo9I GATC 4 cut(s) 78, 149, 724, 1063
LmnI GCTCC 3 cut(s) 749, 976, 1059
Lsp1109I GCAGC 6 cut(s) 301, 304, 778, 781, 784, 820
LweI GCATC 5 cut(s) 44, 164, 199, 666, 948
MabI ACCWGGT 1 cut(s) 814
MaeI CTAG 2 cut(s) 122, 624
MaeII ACGT 2 cut(s) 1068, 1244
MaeIII GTNAC 3 cut(s) 331, 366, 463
MalI GATC 4 cut(s) 80, 151, 726, 1065
MboI GATC 4 cut(s) 78, 149, 724, 1063
MboII GAAGA 3 cut(s) 16, 34, 150
MfeI CAATTG 1 cut(s) 251
MflI RGATCY 1 cut(s) 149
MhlI GDGCHC 1 cut(s) 811
MlsI TGGCCA 1 cut(s) 1104
MluCI AATT 5 cut(s) 251, 533, 554, 998, 1037
MluNI TGGCCA 1 cut(s) 1104
Mly113I GGCGCC 1 cut(s) 281
MlyI GAGTC 4 cut(s) 22, 328, 553, 662
MmeI TCCRAC 1 cut(s) 717
Mox20I TGGCCA 1 cut(s) 1104
MscI TGGCCA 1 cut(s) 1104
MseI TTAA 1 cut(s) 1119
MslI CAYNNNNRTG 1 cut(s) 674
Msp20I TGGCCA 1 cut(s) 1104
MspI CCGG 2 cut(s) 713, 1194
MspR9I CCNGG 5 cut(s) 708, 714, 816, 861, 1194
MunI CAATTG 1 cut(s) 251
Mva1269I GAATGC 1 cut(s) 1147
MvaI CCWGG 3 cut(s) 708, 816, 861
MwoI GCNNNNNNNGC 8 cut(s) 218, 289, 322, 454, 772, 778, 806, 1188
NarI GGCGCC 1 cut(s) 281
NciI CCSGG 2 cut(s) 714, 1194
NcoI CCATGG 1 cut(s) 1047
NdeII GATC 4 cut(s) 78, 149, 724, 1063
NlaIII CATG 4 cut(s) 673, 733, 1051, 1225
NlaIV GGNNCC 6 cut(s) 282, 303, 1044, 1160, 1161, 1192
NmuCI GTSAC 2 cut(s) 331, 463
NspI RCATGY 1 cut(s) 673
PaeI GCATGC 1 cut(s) 673
PagI TCATGA 1 cut(s) 1221
PcsI WCGNNNNNNNCGW 2 cut(s) 518, 1074
PctI GAATGC 1 cut(s) 1147
PfeI GAWTC 5 cut(s) 403, 422, 493, 991, 1081
PflMI CCANNNNNTGG 1 cut(s) 253
PkrI GCNGC 6 cut(s) 291, 294, 768, 771, 774, 835
PleI GAGTC 4 cut(s) 21, 328, 552, 661
PluTI GGCGCC 1 cut(s) 284
PpsI GAGTC 4 cut(s) 21, 328, 552, 661
PpuMI RGGWCCY 1 cut(s) 1159
Psp5II RGGWCCY 1 cut(s) 1159
Psp6I CCWGG 3 cut(s) 706, 814, 859
PspGI CCWGG 3 cut(s) 706, 814, 859
PspN4I GGNNCC 6 cut(s) 282, 303, 1044, 1160, 1161, 1192
PspPI GGNCC 5 cut(s) 710, 715, 839, 1159, 1190
PspPPI RGGWCCY 1 cut(s) 1159
PstNI CAGNNNCTG 1 cut(s) 656
PsuI RGATCY 1 cut(s) 149
RsaI GTAC 3 cut(s) 660, 868, 1044
RsaNI GTAC 3 cut(s) 659, 867, 1043
RseI CAYNNNNRTG 1 cut(s) 674
SaqAI TTAA 1 cut(s) 1119
SatI GCNGC 6 cut(s) 290, 293, 767, 770, 773, 834
Sau3AI GATC 4 cut(s) 78, 149, 724, 1063
Sau96I GGNCC 5 cut(s) 710, 715, 839, 1159, 1190
ScaI AGTACT 1 cut(s) 868
SchI GAGTC 4 cut(s) 22, 328, 553, 662
ScrFI CCNGG 5 cut(s) 708, 714, 816, 861, 1194
SduI GDGCHC 1 cut(s) 811
SexAI ACCWGGT 1 cut(s) 814
SfaNI GCATC 5 cut(s) 44, 164, 199, 666, 948
SfoI GGCGCC 1 cut(s) 282
SinI GGWCC 2 cut(s) 839, 1159
SmiMI CAYNNNNRTG 1 cut(s) 674
SmlI CTYRAG 2 cut(s) 22, 31
SmoI CTYRAG 2 cut(s) 22, 31
SphI GCATGC 1 cut(s) 673
Sse9I AATT 5 cut(s) 251, 533, 554, 998, 1037
SsiI CCGC 6 cut(s) 104, 323, 398, 539, 779, 1199
SspDI GGCGCC 1 cut(s) 280
SspI AATATT 3 cut(s) 410, 689, 908
SspMI CTAG 2 cut(s) 122, 624
StyD4I CCNGG 5 cut(s) 706, 712, 814, 859, 1192
StyI CCWWGG 2 cut(s) 458, 1047
TaaI ACNGT 2 cut(s) 567, 887
TaiI ACGT 2 cut(s) 1071, 1247
TaqI TCGA 3 cut(s) 77, 433, 757
TasI AATT 5 cut(s) 251, 533, 554, 998, 1037
TatI WGTACW 1 cut(s) 866
TfiI GAWTC 5 cut(s) 403, 422, 493, 991, 1081
Tru1I TTAA 1 cut(s) 1119
Tru9I TTAA 1 cut(s) 1119
TscAI CASTG 4 cut(s) 687, 853, 892, 1131
TseFI GTSAC 2 cut(s) 331, 463
TseI GCWGC 6 cut(s) 289, 292, 766, 769, 772, 833
Tsp45I GTSAC 2 cut(s) 331, 463
TspDTI ATGAA 3 cut(s) 17, 1200, 1210
TspGWI ACGGA 1 cut(s) 510
TspRI CASTG 4 cut(s) 687, 853, 892, 1131
Van91I CCANNNNNTGG 1 cut(s) 253
VpaK11BI GGWCC 2 cut(s) 839, 1159
XagI CCTNNNNNAGG 2 cut(s) 705, 1167
XceI RCATGY 1 cut(s) 673
XmiI GTMKAC 2 cut(s) 609, 879
XspI CTAG 2 cut(s) 122, 624
ZrmI AGTACT 1 cut(s) 868
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.