Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr17

Orthogroup ID

OG0005853

Physical Location & Seq

Genomic Coordinates Forward (+)

24642026 .. 24644161

Gene Structure 2136 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2136 bp

ATGGGGAACCATGATGCATTAAGCAATCAAGATAACGGGCAGAGGTTTGGCCTTACGGGGCCCATCTCGCTGGCCGGACCCACCGAGTCTGATGTGATCAAGACTCGTGAATTGGAAAACTACTTGCAAGATGCTGGATTGTATGAGAGTCAGGAAGAGGCTGTGCGCAGGCAGGAGGTTCTTGGCAGCCTCGGCGAGATTGTCAAGGTGTGGGTTAAGACAGTTAGCAAGGCCAAGGGATTCAATGAGCAACTGGTGGATGAAGCAAATGCTTTGATCGTCACCTTTGGATCGTATCGGCTAGGGGTTCATGGCCCAGGAGCAGATATAGACACACTCTGTGTCGGTCCTAAATATGCAACTCGTCAAGAAGATTTCTTCGGCGAGCTACATAGGATGTTGTCTGAGATGCCTCAAGTGACAGAGTTGAACCCTGTACCCGATGCTCACGTCCCGGTCATGAGATTCAAGTTCAGTGGGGTGGCAATAGATCTTCTTTATGCGCAACTAGCACTCTTGGTTATTCCAGAAGACTTGGATATATTACAAGACTCAGTACTACAGGGTGTGGATGAACAGACGGTTCGTAGTCTTAATGGTTTCAGAGTTACAGACCGAATTCTTCGTTTAATCCCAAGCATTCAGAACTTCTGCACGACTTTGAGATGCATGAGGTTATGGGCAAAGCGTCGTTGTGTTTATTCCAACGTTGCGGGATTTCTTGGTGGTATAAATTGGACATTGCTTGTTGCTCGCATATGCCAACTTTACCCGAATGCCCTGCCTAATATGCTAGTGTCTCGGTTCTTTAAGATATATACGCAGTGGCACTGGCCAAATCCTGTCATGCTCTGTGCTATTGAAGCTGGGTCTCTTGGACTGCAGGTCTGGGATCCTATAAGAAACAAAATGGACAGCCTTCATTTGATGCCCATAATTACTCCGGCATATCCCAGCATGAACTCTAGCTACAGCGTGTCTCCGAGTACTAAGTGTATCATGTTAGACGAGTTTCAGAGGGGAAATGATATTTGTAAGGCTATGGAAGCAAACGAGGCTGATTGGGATACCCTTTTTGAGTCTTATGCTTTCTTTGAAGCGTATAAGAATTATTTACAGATAGACATCACTGCAGAAAATGATGATGACTTTACAGCATGGAAAGGCTGGGTTGGGTCCCGTATCCGTCAACTAATGCTGAAGATTGAGAGGCACACATCCGGCAAGCTGCTGTGTCACCCACATCCAGGTGATTTTTCAGACAAATCAAGAGGTTTTCATAGCTCTTTCTTCATGGGTCTACAACGACGTCAAGGAGTTCGTGCCAATGATGGCCAGCGATACTTTGACATAAGAGGAACAATTGAGGAGTTTAAGCAAGCTGATGTCAACCGTTACACATTATGGAAACCTGGAATGGAGATCCGTGTATCCCATGTAAACCGCAGGAGCATCCCAAATTTTGTATTTCCTGGTGGGGTTGGACTTCTGCGCACGTCTAAAGTAACTTGGGGGAGAAGGCAGGGTTCAGAACCAACGGTTTCTGGAGCATCTGAACCACATAAATTATGTGAAGCTAAAACTGATTTAGATGGATCAGATGGCAGGCAGAAGAGAAAGCGAGTGGATGGTAATGTAGAAATTGACTCAAGGCATGCCAAGTCTTTACGTCCCTGCGGTGAAGAATTTCGTGAGGGTAGCCCTTCTATGAGCAGCAGTGTCAATTCATGTTCCATAAAATGTGACAGTATGGATGCAAACAACAATAACTCCGGAAAGACAGGAATCCCAAGAGACATTCCTTATCAGAACGATCAGACTGAATCTTCGTCAAGGTGCAATCCACCAATCGATAGTCTACCTGCTGCTGCAAATACATCAAATTCTAAAGAAGCAGAGAAGCTGGCATTGGAGAAGAAGATTACGGCTACAAAGGAGGGCGCATCAGTTGGTACGCCAATAAACTCTAGCAATGGAGCTGCTGGTCCTTCCAATGATTCATATAATGGGGGTGTAGAGGAACTCGTGGATGAATTCGTGGCGCCATCCTCAAACGGAACCTCTTTTCCACCTGCGGCGCAAAGAAAGCCAATCATCAGGTTCAGTTTCTCTACATTCGTCAAAGCTAGTGGCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0002682 GO:0002683 GO:0003006 GO:0003674 GO:0003824 GO:0004652 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005847 GO:0005849 GO:0006139 GO:0006378 GO:0006396 GO:0006397 GO:0006725 GO:0006807 GO:0007275 GO:0008150 GO:0008152 GO:0008285 GO:0009653 GO:0009791 GO:0009908 GO:0009987 GO:0010467 GO:0016070 GO:0016071 GO:0016740 GO:0016772 GO:0016779 GO:0022414 GO:0031123 GO:0031124 GO:0031347 GO:0031348 GO:0031974 GO:0031981 GO:0032501 GO:0032502 GO:0032991 GO:0034641 GO:0042127 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043631 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0045088 GO:0045824 GO:0046483 GO:0048366 GO:0048367 GO:0048437 GO:0048438 GO:0048441 GO:0048444 GO:0048446 GO:0048449 GO:0048451 GO:0048465 GO:0048519 GO:0048523 GO:0048583 GO:0048585 GO:0048608 GO:0048646 GO:0048731 GO:0048827 GO:0048856 GO:0050776 GO:0050777 GO:0050789 GO:0050794 GO:0061458 GO:0065007 GO:0070013 GO:0070566 GO:0071704 GO:0080134 GO:0090304 GO:0090567 GO:0090696 GO:0090697 GO:0090698 GO:0099402 GO:1901360 GO:1905392 GO:1905393

KEGG Pathways

Metabolic & Signaling

ko03015 map03015

Pfam Domains

Protein Families

NTP_transf_2 PAP_RNA-bind PAP_central

Protein Analysis

712

Amino Acids

79.0

Weight (kDa)

6.4

Isoelectric Point (pI)

41.68

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PAP_NTPase	PF20750	17 - 211	4.1e-76	Poly(A) polymerase nucleotidyltransferase domain
NTP_transf_2	PF01909	77 - 162	2.7e-09	Nucleotidyltransferase domain
PAP_central	PF04928	216 - 360	6.3e-64	Poly(A) polymerase central domain
PAP_RNA-bind	PF04926	364 - 422	1.5e-11	Poly(A) polymerase predicted RNA binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0005853)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G17980 AT1G17980
fragaria_vesca	FvH4_5g16820 FvH4_5g16820 FvH4_5g16820 FvH4_5g16820
malus_domestica	MD06G1222200.v1.1 MD09G1233900.v1.1 MD14G1231300.v1.1 MD17G1263100.v1.1
prunus_persica	Prupe.5G226600_v2.0.a1 Prupe.5G226600_v2.0.a1 Prupe.5G226600_v2.0.a1
pyrus_communis	pycom06g19770 pycom14g19250 pycom17g26340
rosa_chinensis	RchiOBHm_Chr7g0179251
rosa_laevigata	RLG00000005369
rosa_multiflora	Rmu_sc0006939.1_g000005
rosa_roxburghii	Rroxscaffold_3G00274180
rosa_rugosa	Rorug06G0422700 Rorug06G0422800 Rorug06G0422800
rosa_samantha	Rh7AG022000 Rh7BG021900 Rh7CG023200 Rh7DG022500
rosa_wichuraiana	Rw7G001810

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	2080
AatII	GACGTC	1 cut(s)	1312
Acc16I	TGCGCA	3 cut(s)	167, 504, 1493
Acc36I	ACCTGC	3 cut(s)	874, 1870, 2080
AccB1I	GGYRCC	1 cut(s)	2041
AccI	GTMKAC	2 cut(s)	1300, 1858
AccIII	TCCGGA	1 cut(s)	1772
AciI	CCGC	4 cut(s)	713, 1444, 1677, 2075
AclI	AACGTT	1 cut(s)	708
AclWI	GGATC	5 cut(s)	298, 887, 900, 1417, 1603
AcoI	YGGCCR	3 cut(s)	72, 833, 1333
AcsI	RAATTY	5 cut(s)	618, 1459, 1685, 1882, 2033
AcuI	CTGAAG	1 cut(s)	1220
AcyI	GRCGYC	2 cut(s)	1309, 2042
AdeI	CACNNNGTG	1 cut(s)	341
AfaI	GTAC	4 cut(s)	438, 558, 988, 1954
AfiI	CCNNNNNNNGG	1 cut(s)	1247
AgsI	TTSAA	5 cut(s)	244, 430, 469, 863, 1097
AhdI	GACNNNNNGTC	1 cut(s)	884
AjiI	CACGTC	2 cut(s)	451, 1497
AjnI	CCWGG	4 cut(s)	316, 1246, 1411, 1471
AjuI	GAANNNNNNNTTGG	2 cut(s)	1892, 1924
AloI	GAACNNNNNNTCC	2 cut(s)	2050, 2082
Alw26I	GTCTC	4 cut(s)	804, 876, 984, 1788
AlwI	GGATC	5 cut(s)	298, 887, 900, 1417, 1603
Aor13HI	TCCGGA	1 cut(s)	1772
AoxI	GGCC	7 cut(s)	49, 59, 72, 231, 313, 833, 1333
ApaI	GGGCCC	1 cut(s)	63
ApeKI	GCWGC	6 cut(s)	186, 1228, 1713, 1865, 1868, 1979
ApoI	RAATTY	5 cut(s)	618, 1459, 1685, 1882, 2033
ArsI	GACNNNNNNTTYG	2 cut(s)	1810, 1842
Asp700I	GAANNNNTTC	1 cut(s)	1686
AspLEI	GCGC	6 cut(s)	168, 505, 1494, 1943, 2044, 2080
AspS9I	GGNCC	7 cut(s)	59, 60, 77, 314, 347, 1176, 1985
AsuC2I	CCSGG	1 cut(s)	455
AsuHPI	GGTGA	4 cut(s)	274, 1229, 1262, 1691
AvaII	GGWCC	4 cut(s)	77, 347, 1176, 1985
BaeGI	GKGCMC	1 cut(s)	63
BalI	TGGCCA	2 cut(s)	835, 1335
BamHI	GGATCC	1 cut(s)	892
BanI	GGYRCC	1 cut(s)	2041
BanII	GRGCYC	1 cut(s)	63
BauI	CACGAG	2 cut(s)	105, 2024
BbsI	GAAGAC	1 cut(s)	537
BbvI	GCAGC	6 cut(s)	198, 1215, 1725, 1852, 1855, 1966
BccI	CCATC	6 cut(s)	71, 1325, 1586, 1595, 1622, 2053
BceAI	ACGGC	1 cut(s)	1941
BcgI	CGANNNNNNTGC	2 cut(s)	763, 797
BciT130I	CCWGG	4 cut(s)	318, 1248, 1413, 1473
BciVI	GTATCC	3 cut(s)	1060, 1193, 1441
BclI	TGATCA	1 cut(s)	96
BcnI	CCSGG	1 cut(s)	455
BcoDI	GTCTC	4 cut(s)	804, 876, 984, 1788
BfaI	CTAG	6 cut(s)	302, 509, 794, 966, 1968, 2127
BfmI	CTRYAG	4 cut(s)	560, 881, 970, 1131
BfoI	RGCGCY	1 cut(s)	2045
BfuAI	ACCTGC	3 cut(s)	874, 1870, 2080
BfuI	GTATCC	3 cut(s)	1060, 1193, 1441
BglII	AGATCT	1 cut(s)	490
BisI	GCNGC	7 cut(s)	187, 1229, 1714, 1866, 1869, 1980, 2076
BlsI	GCNGC	7 cut(s)	188, 1230, 1715, 1867, 1870, 1981, 2077
BmcAI	AGTACT	2 cut(s)	558, 988
Bme1390I	CCNGG	5 cut(s)	318, 455, 1248, 1413, 1473
Bme18I	GGWCC	4 cut(s)	77, 347, 1176, 1985
BmeRI	GACNNNNNGTC	1 cut(s)	884
BmgBI	CACGTC	2 cut(s)	451, 1497
BmgT120I	GGNCC	7 cut(s)	59, 60, 77, 314, 347, 1176, 1985
BmiI	GGNNCC	9 cut(s)	8, 60, 61, 79, 894, 1177, 1178, 2043, 2059
BmrFI	CCNGG	5 cut(s)	318, 455, 1248, 1413, 1473
BpiI	GAAGAC	1 cut(s)	537
BpmI	CTGGAG	1 cut(s)	1566
BpuEI	CTTGAG	2 cut(s)	399, 1633
BpuMI	CCSGG	1 cut(s)	455
Bsa29I	ATCGAT	1 cut(s)	1851
BsaBI	GATNNNNATC	1 cut(s)	1124
BsaHI	GRCGYC	2 cut(s)	1309, 2042
BsaI	GGTCTC	1 cut(s)	876
BsaJI	CCNNGG	3 cut(s)	190, 234, 316
BsaWI	WCCGGW	1 cut(s)	1772
BsaXI	ACNNNNNCTCC	2 cut(s)	1754, 1784
Bsc4I	CCNNNNNNNGG	1 cut(s)	1247
Bse1I	ACTGG	2 cut(s)	258, 836
Bse3DI	GCAATG	2 cut(s)	740, 1978
Bse8I	GATNNNNATC	1 cut(s)	1124
BseAI	TCCGGA	1 cut(s)	1772
BseBI	CCWGG	4 cut(s)	318, 1248, 1413, 1473
BseCI	ATCGAT	1 cut(s)	1851
BseDI	CCNNGG	3 cut(s)	190, 234, 316
BseJI	GATNNNNATC	1 cut(s)	1124
BseLI	CCNNNNNNNGG	1 cut(s)	1247
BseMI	GCAATG	2 cut(s)	740, 1978
BseMII	CTCAG	2 cut(s)	396, 567
BseNI	ACTGG	2 cut(s)	258, 836
BseRI	GAGGAG	1 cut(s)	1382
BseSI	GKGCMC	1 cut(s)	63
BseXI	GCAGC	6 cut(s)	198, 1215, 1725, 1852, 1855, 1966
BseYI	CCCAGC	3 cut(s)	866, 953, 1167
BsgI	GTGCAG	1 cut(s)	637
BshFI	GGCC	7 cut(s)	51, 61, 74, 233, 315, 835, 1335
BshNI	GGYRCC	1 cut(s)	2041
BshVI	ATCGAT	1 cut(s)	1851
BsiSI	CCGG	5 cut(s)	75, 455, 944, 1221, 1773
BslFI	GGGAC	3 cut(s)	437, 1162, 1656
BslI	CCNNNNNNNGG	1 cut(s)	1247
BsmAI	GTCTC	4 cut(s)	804, 876, 984, 1788
BsmFI	GGGAC	3 cut(s)	437, 1162, 1656
BsmI	GAATGC	2 cut(s)	639, 781
BsnI	GGCC	7 cut(s)	51, 61, 74, 233, 315, 835, 1335
Bso31I	GGTCTC	1 cut(s)	876
Bsp120I	GGGCCC	1 cut(s)	59
Bsp1286I	GDGCHC	1 cut(s)	63
Bsp13I	TCCGGA	1 cut(s)	1772
Bsp143I	GATC	8 cut(s)	96, 276, 290, 490, 892, 1422, 1595, 1813
BspACI	CCGC	4 cut(s)	713, 1444, 1677, 2075
BspANI	GGCC	7 cut(s)	51, 61, 74, 233, 315, 835, 1335
BspCNI	CTCAG	2 cut(s)	397, 566
BspDI	ATCGAT	1 cut(s)	1851
BspEI	TCCGGA	1 cut(s)	1772
BspHI	TCATGA	1 cut(s)	459
BspLI	GGNNCC	9 cut(s)	8, 60, 61, 79, 894, 1177, 1178, 2043, 2059
BspMAI	CTGCAG	2 cut(s)	885, 1135
BspMI	ACCTGC	3 cut(s)	874, 1870, 2080
BspPI	GGATC	5 cut(s)	298, 887, 900, 1417, 1603
BspT107I	GGYRCC	1 cut(s)	2041
BspTNI	GGTCTC	1 cut(s)	876
BsrDI	GCAATG	2 cut(s)	740, 1978
BsrI	ACTGG	2 cut(s)	258, 836
BssECI	CCNNGG	3 cut(s)	190, 234, 316
BssMI	GATC	8 cut(s)	96, 276, 290, 490, 892, 1422, 1595, 1813
BssNI	GRCGYC	2 cut(s)	1309, 2042
BssSI	CACGAG	2 cut(s)	105, 2024
BssT1I	CCWWGG	1 cut(s)	234
Bst2BI	CACGAG	2 cut(s)	105, 2024
Bst2UI	CCWGG	4 cut(s)	318, 1248, 1413, 1473
Bst4CI	ACNGT	5 cut(s)	223, 583, 1394, 1540, 1748
Bst6I	CTCTTC	2 cut(s)	150, 1607
BstACI	GRCGYC	2 cut(s)	1309, 2042
BstDEI	CTNAG	3 cut(s)	405, 553, 990
BstH2I	RGCGCY	1 cut(s)	2045
BstHHI	GCGC	6 cut(s)	168, 505, 1494, 1943, 2044, 2080
BstKTI	GATC	8 cut(s)	99, 279, 293, 493, 895, 1425, 1598, 1816
BstMAI	GTCTC	4 cut(s)	804, 876, 984, 1788
BstMBI	GATC	8 cut(s)	96, 276, 290, 490, 892, 1422, 1595, 1813
BstMWI	GCNNNNNNNGC	8 cut(s)	67, 192, 509, 790, 863, 1046, 1055, 2086
BstNI	CCWGG	4 cut(s)	318, 1248, 1413, 1473
BstNSI	RCATGY	1 cut(s)	1658
BstSCI	CCNGG	5 cut(s)	316, 453, 1246, 1411, 1471
BstSFI	CTRYAG	4 cut(s)	560, 881, 970, 1131
BstSLI	GKGCMC	1 cut(s)	63
BstV1I	GCAGC	6 cut(s)	198, 1215, 1725, 1852, 1855, 1966
BstV2I	GAAGAC	1 cut(s)	537
BstX2I	RGATCY	3 cut(s)	490, 892, 1422
BstXI	CCANNNNNNTGG	1 cut(s)	70
BstYI	RGATCY	3 cut(s)	490, 892, 1422
Bsu15I	ATCGAT	1 cut(s)	1851
BsuI	GTATCC	3 cut(s)	1060, 1193, 1441
BsuRI	GGCC	7 cut(s)	51, 61, 74, 233, 315, 835, 1335
BsuTUI	ATCGAT	1 cut(s)	1851
BtrI	CACGTC	2 cut(s)	451, 1497
BtsI	GCAGTG	3 cut(s)	830, 1128, 1723
BtsIMutI	CAGTG	5 cut(s)	481, 829, 830, 1128, 1723
BveI	ACCTGC	3 cut(s)	874, 1870, 2080
CciI	TCATGA	1 cut(s)	459
CfoI	GCGC	6 cut(s)	168, 505, 1494, 1943, 2044, 2080
Cfr13I	GGNCC	7 cut(s)	59, 60, 77, 314, 347, 1176, 1985
ClaI	ATCGAT	1 cut(s)	1851
CseI	GACGC	1 cut(s)	677
Csp6I	GTAC	4 cut(s)	437, 557, 987, 1953
CspCI	CAANNNNNGTGG	3 cut(s)	457, 492, 2110
CviQI	GTAC	4 cut(s)	437, 557, 987, 1953
DdeI	CTNAG	3 cut(s)	405, 553, 990
DinI	GGCGCC	1 cut(s)	2043
DpnI	GATC	8 cut(s)	98, 278, 292, 492, 894, 1424, 1597, 1815
DpnII	GATC	8 cut(s)	96, 276, 290, 490, 892, 1422, 1595, 1813
DraIII	CACNNNGTG	1 cut(s)	341
DriI	GACNNNNNGTC	1 cut(s)	884
EaeI	YGGCCR	3 cut(s)	72, 833, 1333
Eam1104I	CTCTTC	2 cut(s)	150, 1607
Eam1105I	GACNNNNNGTC	1 cut(s)	884
EarI	CTCTTC	2 cut(s)	150, 1607
Eco130I	CCWWGG	1 cut(s)	234
Eco24I	GRGCYC	1 cut(s)	63
Eco31I	GGTCTC	1 cut(s)	876
Eco47I	GGWCC	4 cut(s)	77, 347, 1176, 1985
Eco57I	CTGAAG	1 cut(s)	1220
EcoO109I	RGGNCCY	2 cut(s)	59, 1176
EcoRI	GAATTC	2 cut(s)	618, 2033
EcoRII	CCWGG	4 cut(s)	316, 1246, 1411, 1471
EcoT14I	CCWWGG	1 cut(s)	234
EcoT22I	ATGCAT	2 cut(s)	19, 671
EcoT38I	GRGCYC	1 cut(s)	63
EgeI	GGCGCC	1 cut(s)	2043
EheI	GGCGCC	1 cut(s)	2043
ErhI	CCWWGG	1 cut(s)	234
FaqI	GGGAC	3 cut(s)	437, 1162, 1656
FauI	CCCGC	1 cut(s)	706
FauNDI	CATATG	1 cut(s)	758
FbaI	TGATCA	1 cut(s)	96
FblI	GTMKAC	2 cut(s)	1300, 1858
Fnu4HI	GCNGC	7 cut(s)	187, 1229, 1714, 1866, 1869, 1980, 2076
FriOI	GRGCYC	1 cut(s)	63
Fsp4HI	GCNGC	7 cut(s)	187, 1229, 1714, 1866, 1869, 1980, 2076
FspBI	CTAG	6 cut(s)	302, 509, 794, 966, 1968, 2127
FspI	TGCGCA	3 cut(s)	167, 504, 1493
GlaI	GCGC	6 cut(s)	167, 504, 1493, 1942, 2043, 2079
GluI	GCNGC	7 cut(s)	187, 1229, 1714, 1866, 1869, 1980, 2076
GsaI	CCCAGC	3 cut(s)	870, 957, 1171
GsuI	CTGGAG	1 cut(s)	1566
HaeII	RGCGCY	1 cut(s)	2045
HaeIII	GGCC	7 cut(s)	51, 61, 74, 233, 315, 835, 1335
HapII	CCGG	5 cut(s)	75, 455, 944, 1221, 1773
HgaI	GACGC	1 cut(s)	677
HhaI	GCGC	6 cut(s)	168, 505, 1494, 1943, 2044, 2080
Hin1I	GRCGYC	2 cut(s)	1309, 2042
Hin6I	GCGC	6 cut(s)	166, 503, 1492, 1941, 2042, 2078
HinP1I	GCGC	6 cut(s)	166, 503, 1492, 1941, 2042, 2078
HincII	GTYRAC	2 cut(s)	1190, 1390
HindII	GTYRAC	2 cut(s)	1190, 1390
HpaII	CCGG	5 cut(s)	75, 455, 944, 1221, 1773
HphI	GGTGA	4 cut(s)	274, 1229, 1262, 1691
Hpy166II	GTNNAC	5 cut(s)	1190, 1301, 1390, 1441, 1859
Hpy8I	GTNNAC	5 cut(s)	1190, 1301, 1390, 1441, 1859
Hpy99I	CGWCG	2 cut(s)	693, 1311
HpyAV	CCTTC	4 cut(s)	929, 1512, 1713, 1998
HpyCH4III	ACNGT	5 cut(s)	223, 583, 1394, 1540, 1748
HpyCH4IV	ACGT	5 cut(s)	450, 708, 1309, 1496, 1669
HpyF10VI	GCNNNNNNNGC	8 cut(s)	67, 192, 509, 790, 863, 1046, 1055, 2086
HpyF3I	CTNAG	3 cut(s)	405, 553, 990
HpySE526I	ACGT	5 cut(s)	450, 708, 1309, 1496, 1669
Hsp92I	GRCGYC	2 cut(s)	1309, 2042
HspAI	GCGC	6 cut(s)	166, 503, 1492, 1941, 2042, 2078
KasI	GGCGCC	1 cut(s)	2041
KflI	GGGWCCC	1 cut(s)	1176
Kpn2I	TCCGGA	1 cut(s)	1772
Ksp22I	TGATCA	1 cut(s)	96
Kzo9I	GATC	8 cut(s)	96, 276, 290, 490, 892, 1422, 1595, 1813
LmnI	GCTCC	4 cut(s)	320, 1449, 1547, 1976
Lsp1109I	GCAGC	6 cut(s)	198, 1215, 1725, 1852, 1855, 1966
MaeI	CTAG	6 cut(s)	302, 509, 794, 966, 1968, 2127
MaeII	ACGT	5 cut(s)	450, 708, 1309, 1496, 1669
MaeIII	GTNAC	7 cut(s)	280, 418, 607, 1235, 1394, 1504, 1742
MalI	GATC	8 cut(s)	98, 278, 292, 492, 894, 1424, 1597, 1815
MboI	GATC	8 cut(s)	96, 276, 290, 490, 892, 1422, 1595, 1813
MfeI	CAATTG	1 cut(s)	1362
MflI	RGATCY	3 cut(s)	490, 892, 1422
MhlI	GDGCHC	1 cut(s)	63
MlsI	TGGCCA	2 cut(s)	835, 1335
MluNI	TGGCCA	2 cut(s)	835, 1335
Mly113I	GGCGCC	1 cut(s)	2042
MlyI	GAGTC	6 cut(s)	95, 97, 157, 545, 1088, 1640
MmeI	TCCRAC	2 cut(s)	729, 1462
Mox20I	TGGCCA	2 cut(s)	835, 1335
Mph1103I	ATGCAT	2 cut(s)	19, 671
MroI	TCCGGA	1 cut(s)	1772
MroXI	GAANNNNTTC	1 cut(s)	1686
MscI	TGGCCA	2 cut(s)	835, 1335
MseI	TTAA	6 cut(s)	20, 216, 594, 629, 810, 1374
MslI	CAYNNNNRTG	1 cut(s)	1248
Msp20I	TGGCCA	2 cut(s)	835, 1335
MspI	CCGG	5 cut(s)	75, 455, 944, 1221, 1773
MspR9I	CCNGG	5 cut(s)	318, 455, 1248, 1413, 1473
MunI	CAATTG	1 cut(s)	1362
Mva1269I	GAATGC	2 cut(s)	639, 781
MvaI	CCWGG	4 cut(s)	318, 1248, 1413, 1473
MwoI	GCNNNNNNNGC	8 cut(s)	67, 192, 509, 790, 863, 1046, 1055, 2086
NarI	GGCGCC	1 cut(s)	2042
NciI	CCSGG	1 cut(s)	455
NdeI	CATATG	1 cut(s)	758
NdeII	GATC	8 cut(s)	96, 276, 290, 490, 892, 1422, 1595, 1813
NlaIV	GGNNCC	9 cut(s)	8, 60, 61, 79, 894, 1177, 1178, 2043, 2059
NmeAIII	GCCGAG	1 cut(s)	171
NmuCI	GTSAC	4 cut(s)	280, 418, 1235, 1742
NsbI	TGCGCA	3 cut(s)	167, 504, 1493
NsiI	ATGCAT	2 cut(s)	19, 671
NspI	RCATGY	1 cut(s)	1658
PaeI	GCATGC	1 cut(s)	1658
PagI	TCATGA	1 cut(s)	459
PaqCI	CACCTGC	1 cut(s)	2080
PctI	GAATGC	2 cut(s)	639, 781
PdmI	GAANNNNTTC	1 cut(s)	1686
PfeI	GAWTC	5 cut(s)	240, 465, 1785, 1823, 1997
PkrI	GCNGC	7 cut(s)	188, 1230, 1715, 1867, 1870, 1981, 2077
PleI	GAGTC	6 cut(s)	94, 97, 156, 545, 1087, 1640
PluTI	GGCGCC	1 cut(s)	2045
PpsI	GAGTC	6 cut(s)	94, 97, 156, 545, 1087, 1640
PpuMI	RGGWCCY	1 cut(s)	1176
Psp1406I	AACGTT	1 cut(s)	708
Psp5II	RGGWCCY	1 cut(s)	1176
Psp6I	CCWGG	4 cut(s)	316, 1246, 1411, 1471
PspFI	CCCAGC	3 cut(s)	866, 953, 1167
PspGI	CCWGG	4 cut(s)	316, 1246, 1411, 1471
PspN4I	GGNNCC	9 cut(s)	8, 60, 61, 79, 894, 1177, 1178, 2043, 2059
PspOMI	GGGCCC	1 cut(s)	59
PspPI	GGNCC	7 cut(s)	59, 60, 77, 314, 347, 1176, 1985
PspPPI	RGGWCCY	1 cut(s)	1176
PsrI	GAACNNNNNNTAC	2 cut(s)	953, 985
PstI	CTGCAG	2 cut(s)	885, 1135
PsuI	RGATCY	3 cut(s)	490, 892, 1422
RsaI	GTAC	4 cut(s)	438, 558, 988, 1954
RsaNI	GTAC	4 cut(s)	437, 557, 987, 1953
RseI	CAYNNNNRTG	1 cut(s)	1248
SaqAI	TTAA	6 cut(s)	20, 216, 594, 629, 810, 1374
SatI	GCNGC	7 cut(s)	187, 1229, 1714, 1866, 1869, 1980, 2076
Sau3AI	GATC	8 cut(s)	96, 276, 290, 490, 892, 1422, 1595, 1813
Sau96I	GGNCC	7 cut(s)	59, 60, 77, 314, 347, 1176, 1985
ScaI	AGTACT	2 cut(s)	558, 988
SchI	GAGTC	6 cut(s)	95, 97, 157, 545, 1088, 1640
ScrFI	CCNGG	5 cut(s)	318, 455, 1248, 1413, 1473
SduI	GDGCHC	1 cut(s)	63
SfcI	CTRYAG	4 cut(s)	560, 881, 970, 1131
SfoI	GGCGCC	1 cut(s)	2043
SinI	GGWCC	4 cut(s)	77, 347, 1176, 1985
SmiMI	CAYNNNNRTG	1 cut(s)	1248
SmlI	CTYRAG	2 cut(s)	414, 1648
SmoI	CTYRAG	2 cut(s)	414, 1648
SphI	GCATGC	1 cut(s)	1658
SsiI	CCGC	4 cut(s)	713, 1444, 1677, 2075
SspDI	GGCGCC	1 cut(s)	2041
SspMI	CTAG	6 cut(s)	302, 509, 794, 966, 1968, 2127
StyD4I	CCNGG	5 cut(s)	316, 453, 1246, 1411, 1471
StyI	CCWWGG	1 cut(s)	234
TaaI	ACNGT	5 cut(s)	223, 583, 1394, 1540, 1748
TaiI	ACGT	5 cut(s)	453, 711, 1312, 1499, 1672
TaqI	TCGA	1 cut(s)	1851
TaqII	GACCGA	2 cut(s)	335, 630
TatI	WGTACW	2 cut(s)	556, 986
TauI	GCSGC	1 cut(s)	2078
TfiI	GAWTC	5 cut(s)	240, 465, 1785, 1823, 1997
Tru1I	TTAA	6 cut(s)	20, 216, 594, 629, 810, 1374
Tru9I	TTAA	6 cut(s)	20, 216, 594, 629, 810, 1374
TscAI	CASTG	5 cut(s)	481, 830, 836, 1135, 1723
TseFI	GTSAC	4 cut(s)	280, 418, 1235, 1742
TseI	GCWGC	6 cut(s)	186, 1228, 1713, 1865, 1868, 1979
Tsp45I	GTSAC	4 cut(s)	280, 418, 1235, 1742
TspGWI	ACGGA	3 cut(s)	1175, 1415, 2070
TspRI	CASTG	5 cut(s)	481, 830, 836, 1135, 1723
VpaK11BI	GGWCC	4 cut(s)	77, 347, 1176, 1985
XapI	RAATTY	5 cut(s)	618, 1459, 1685, 1882, 2033
XceI	RCATGY	1 cut(s)	1658
XmiI	GTMKAC	2 cut(s)	1300, 1858
XmnI	GAANNNNTTC	1 cut(s)	1686
XspI	CTAG	6 cut(s)	302, 509, 794, 966, 1968, 2127
ZraI	GACGTC	1 cut(s)	1310
ZrmI	AGTACT	2 cut(s)	558, 988
Zsp2I	ATGCAT	2 cut(s)	19, 671

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.