RchiOBHm_Chr6g0254811

hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0012556
Physical Location & Seq
Forward (+)
9723586 .. 9728159
4574 bp
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UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 285 bp
ATGGTAGGGTATGGGACTAAAGAGTTAGATCTTAAGGGAAAAATTGTAGTCCCTGGATTTATTGATTCCCATGTGCATCTGATCTTTGGTGGACTGCAGATGATGCGTGTAGAACTACATGGCATAAACTCAAAAGATGAATTTGTGAAGAGGATCAAAGAAGCTGTGAGAAGGGTCTTTACTGATCGAGCTTATTCAGCAGATGCAAGTACAAGGTGTGCCGGTATGGTTTCTTTGATGCCTCTCTTGTTGGTCCTTTTCTTGCTGCTGTTTGAGAATCATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Amidohydro_3

Protein Analysis

94

Amino Acids

10.59

Weight (kDa)

8.89

Isoelectric Point (pI)

18.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Amidohydro_3 PF07969 8 - 58 2.9e-11 Amidohydrolase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 161
AcsI RAATTY 1 cut(s) 140
AfaI GTAC 1 cut(s) 211
AflII CTTAAG 1 cut(s) 32
AjnI CCWGG 1 cut(s) 52
AluBI AGCT 2 cut(s) 164, 191
AluI AGCT 2 cut(s) 164, 191
AlwI GGATC 1 cut(s) 161
ApeKI GCWGC 1 cut(s) 265
ApoI RAATTY 1 cut(s) 140
AspS9I GGNCC 1 cut(s) 253
AvaII GGWCC 1 cut(s) 253
BarI GAAGNNNNNNTAC 2 cut(s) 163, 195
BbvI GCAGC 1 cut(s) 252
BciT130I CCWGG 1 cut(s) 54
BfmI CTRYAG 1 cut(s) 95
BfrI CTTAAG 1 cut(s) 32
BglII AGATCT 1 cut(s) 28
BisI GCNGC 1 cut(s) 266
BlsI GCNGC 1 cut(s) 267
Bme1390I CCNGG 1 cut(s) 54
Bme18I GGWCC 1 cut(s) 253
BmgT120I GGNCC 1 cut(s) 253
BmrFI CCNGG 1 cut(s) 54
BmsI GCATC 4 cut(s) 85, 93, 193, 228
BsaJI CCNNGG 1 cut(s) 52
Bse118I RCCGGY 1 cut(s) 221
BseBI CCWGG 1 cut(s) 54
BseDI CCNNGG 1 cut(s) 52
BseXI GCAGC 1 cut(s) 252
BsiSI CCGG 1 cut(s) 222
BslFI GGGAC 2 cut(s) 28, 35
BsmFI GGGAC 2 cut(s) 28, 35
Bsp143I GATC 4 cut(s) 28, 81, 153, 184
BspMAI CTGCAG 1 cut(s) 99
BspPI GGATC 1 cut(s) 161
BspTI CTTAAG 1 cut(s) 32
BsrFI RCCGGY 1 cut(s) 221
BssAI RCCGGY 1 cut(s) 221
BssECI CCNNGG 1 cut(s) 52
BssMI GATC 4 cut(s) 28, 81, 153, 184
Bst2UI CCWGG 1 cut(s) 54
Bst6I CTCTTC 1 cut(s) 143
BstAFI CTTAAG 1 cut(s) 32
BstAPI GCANNNNNTGC 1 cut(s) 103
BstKTI GATC 4 cut(s) 31, 84, 156, 187
BstMBI GATC 4 cut(s) 28, 81, 153, 184
BstMWI GCNNNNNNNGC 2 cut(s) 103, 197
BstNI CCWGG 1 cut(s) 54
BstSCI CCNGG 1 cut(s) 52
BstSFI CTRYAG 1 cut(s) 95
BstV1I GCAGC 1 cut(s) 252
BstX2I RGATCY 1 cut(s) 28
BstYI RGATCY 1 cut(s) 28
Cfr10I RCCGGY 1 cut(s) 221
Cfr13I GGNCC 1 cut(s) 253
Csp6I GTAC 1 cut(s) 210
CviAII CATG 2 cut(s) 71, 119
CviJI RGCY 2 cut(s) 164, 191
CviKI_1 RGCY 2 cut(s) 164, 191
CviQI GTAC 1 cut(s) 210
DpnI GATC 4 cut(s) 30, 83, 155, 186
DpnII GATC 4 cut(s) 28, 81, 153, 184
Eam1104I CTCTTC 1 cut(s) 143
EarI CTCTTC 1 cut(s) 143
Eco47I GGWCC 1 cut(s) 253
EcoRII CCWGG 1 cut(s) 52
FaeI CATG 2 cut(s) 74, 122
FaiI YATR 5 cut(s) 12, 72, 120, 125, 227
FaqI GGGAC 2 cut(s) 28, 35
FatI CATG 2 cut(s) 70, 118
Fnu4HI GCNGC 1 cut(s) 266
Fsp4HI GCNGC 1 cut(s) 266
GluI GCNGC 1 cut(s) 266
HapII CCGG 1 cut(s) 222
Hin1II CATG 2 cut(s) 74, 122
HinfI GANTC 2 cut(s) 65, 277
HpaII CCGG 1 cut(s) 222
Hpy166II GTNNAC 1 cut(s) 92
Hpy188I TCNGA 1 cut(s) 81
Hpy8I GTNNAC 1 cut(s) 92
HpyAV CCTTC 1 cut(s) 165
HpyCH4V TGCA 3 cut(s) 76, 97, 206
HpyF10VI GCNNNNNNNGC 2 cut(s) 103, 197
Hsp92II CATG 2 cut(s) 74, 122
Kzo9I GATC 4 cut(s) 28, 81, 153, 184
LpnPI CCDG 3 cut(s) 39, 66, 235
Lsp1109I GCAGC 1 cut(s) 252
LweI GCATC 4 cut(s) 85, 93, 193, 228
MalI GATC 4 cut(s) 30, 83, 155, 186
MboI GATC 4 cut(s) 28, 81, 153, 184
MboII GAAGA 1 cut(s) 160
MflI RGATCY 1 cut(s) 28
MluCI AATT 2 cut(s) 42, 140
MnlI CCTC 2 cut(s) 144, 252
MseI TTAA 2 cut(s) 33, 283
MspCI CTTAAG 1 cut(s) 32
MspI CCGG 1 cut(s) 222
MspR9I CCNGG 1 cut(s) 54
MvaI CCWGG 1 cut(s) 54
MwoI GCNNNNNNNGC 2 cut(s) 103, 197
NdeII GATC 4 cut(s) 28, 81, 153, 184
NlaIII CATG 2 cut(s) 74, 122
PfeI GAWTC 2 cut(s) 65, 277
PkrI GCNGC 1 cut(s) 267
Psp6I CCWGG 1 cut(s) 52
PspGI CCWGG 1 cut(s) 52
PspPI GGNCC 1 cut(s) 253
PstI CTGCAG 1 cut(s) 99
PsuI RGATCY 1 cut(s) 28
RsaI GTAC 1 cut(s) 211
RsaNI GTAC 1 cut(s) 210
SaqAI TTAA 2 cut(s) 33, 283
SatI GCNGC 1 cut(s) 266
Sau3AI GATC 4 cut(s) 28, 81, 153, 184
Sau96I GGNCC 1 cut(s) 253
ScrFI CCNGG 1 cut(s) 54
SetI ASST 3 cut(s) 166, 193, 218
SfaNI GCATC 4 cut(s) 85, 93, 193, 228
SfcI CTRYAG 1 cut(s) 95
SinI GGWCC 1 cut(s) 253
SmlI CTYRAG 1 cut(s) 32
SmoI CTYRAG 1 cut(s) 32
Sse9I AATT 2 cut(s) 42, 140
StyD4I CCNGG 1 cut(s) 52
TaqI TCGA 1 cut(s) 187
TasI AATT 2 cut(s) 42, 140
TatI WGTACW 1 cut(s) 209
TfiI GAWTC 2 cut(s) 65, 277
Tru1I TTAA 2 cut(s) 33, 283
Tru9I TTAA 2 cut(s) 33, 283
TseI GCWGC 1 cut(s) 265
TspDTI ATGAA 1 cut(s) 153
Vha464I CTTAAG 1 cut(s) 32
VpaK11BI GGWCC 1 cut(s) 253
XapI RAATTY 1 cut(s) 140
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.