Rw6G010670

hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0012556
Physical Location & Seq
Forward (+)
18472514 .. 18496573
24060 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G010670.1

Sequence Viewer

Length: 396 bp
ATGCAGGATATGGTAGGTTATGGGACTAAAGAATTAGATCTTAAGGGAAAAATTGTAGTCCCTGGATTTATTGATTCCCATGTGCATCTGATCTTTGGTGGACTGCAGATGATGCGTGTAGAACTACATGGCATAAACTCAAAAGATGAATTTGTGAAGAGGATCAAAGAAGCTGTGAGAAGGGTCTTTACTGATCGAGCTTATTCAGCAGATGCAAGTACAAGGTGTGCCGGTGCAATCTTCCTCGAGCTCCCCAGCTTTTTGCCGACCAGTATTCGTCGGCATAAGTTGCTGAGTGATAAAGTTGCACTTGCCCCTGAAGATGAAAGGCTTGGGCTGTCCGTATCCGAATCCAAATTCAAAATCCGAGAAGCACAATTCCACGCGTCACACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Amidohydro_3

Protein Analysis

131

Amino Acids

14.74

Weight (kDa)

9.13

Isoelectric Point (pI)

28.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Amidohydro_3 PF07969 11 - 61 6.4e-11 Amidohydrolase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 386
AclWI GGATC 1 cut(s) 170
AcsI RAATTY 2 cut(s) 149, 356
AcuI CTGAAG 1 cut(s) 339
AfaI GTAC 1 cut(s) 220
AflII CTTAAG 1 cut(s) 41
AflIII ACRYGT 1 cut(s) 384
AgsI TTSAA 1 cut(s) 361
AjnI CCWGG 1 cut(s) 61
AluBI AGCT 4 cut(s) 173, 200, 250, 258
AluI AGCT 4 cut(s) 173, 200, 250, 258
Alw21I GWGCWC 1 cut(s) 252
AlwI GGATC 1 cut(s) 170
Ama87I CYCGRG 1 cut(s) 245
ApoI RAATTY 2 cut(s) 149, 356
AvaI CYCGRG 1 cut(s) 245
BanII GRGCYC 1 cut(s) 252
BarI GAAGNNNNNNTAC 2 cut(s) 172, 204
Bbv12I GWGCWC 1 cut(s) 252
BciT130I CCWGG 1 cut(s) 63
BciVI GTATCC 1 cut(s) 355
BfmI CTRYAG 1 cut(s) 104
BfrI CTTAAG 1 cut(s) 41
BfuI GTATCC 1 cut(s) 355
BglII AGATCT 1 cut(s) 37
Bme1390I CCNGG 1 cut(s) 63
BmeT110I CYCGRG 1 cut(s) 245
BmrFI CCNGG 1 cut(s) 63
BmsI GCATC 3 cut(s) 94, 102, 202
BsaJI CCNNGG 1 cut(s) 61
Bse118I RCCGGY 1 cut(s) 230
Bse1I ACTGG 1 cut(s) 270
BseBI CCWGG 1 cut(s) 63
BseDI CCNNGG 1 cut(s) 61
BseMII CTCAG 1 cut(s) 284
BseNI ACTGG 1 cut(s) 270
BseYI CCCAGC 1 cut(s) 254
Bsh1236I CGCG 1 cut(s) 386
BsiHKAI GWGCWC 1 cut(s) 252
BsiHKCI CYCGRG 1 cut(s) 245
BsiSI CCGG 1 cut(s) 231
BslFI GGGAC 2 cut(s) 37, 44
BsmFI GGGAC 2 cut(s) 37, 44
BsoBI CYCGRG 1 cut(s) 245
Bsp1286I GDGCHC 1 cut(s) 252
Bsp143I GATC 4 cut(s) 37, 90, 162, 193
BspCNI CTCAG 1 cut(s) 285
BspFNI CGCG 1 cut(s) 386
BspMAI CTGCAG 1 cut(s) 108
BspPI GGATC 1 cut(s) 170
BspTI CTTAAG 1 cut(s) 41
BsrFI RCCGGY 1 cut(s) 230
BsrI ACTGG 1 cut(s) 270
BssAI RCCGGY 1 cut(s) 230
BssECI CCNNGG 1 cut(s) 61
BssMI GATC 4 cut(s) 37, 90, 162, 193
Bst2UI CCWGG 1 cut(s) 63
Bst6I CTCTTC 1 cut(s) 152
BstAFI CTTAAG 1 cut(s) 41
BstAPI GCANNNNNTGC 2 cut(s) 112, 289
BstDEI CTNAG 1 cut(s) 293
BstFNI CGCG 1 cut(s) 386
BstKTI GATC 4 cut(s) 40, 93, 165, 196
BstMBI GATC 4 cut(s) 37, 90, 162, 193
BstMWI GCNNNNNNNGC 3 cut(s) 112, 206, 289
BstNI CCWGG 1 cut(s) 63
BstSCI CCNGG 1 cut(s) 61
BstSFI CTRYAG 1 cut(s) 104
BstUI CGCG 1 cut(s) 386
BstX2I RGATCY 1 cut(s) 37
BstYI RGATCY 1 cut(s) 37
BsuI GTATCC 1 cut(s) 355
Cfr10I RCCGGY 1 cut(s) 230
CseI GACGC 1 cut(s) 375
Csp6I GTAC 1 cut(s) 219
CviAII CATG 2 cut(s) 80, 128
CviJI RGCY 6 cut(s) 173, 200, 250, 258, 331, 337
CviKI_1 RGCY 6 cut(s) 173, 200, 250, 258, 331, 337
CviQI GTAC 1 cut(s) 219
DdeI CTNAG 1 cut(s) 293
DpnI GATC 4 cut(s) 39, 92, 164, 195
DpnII GATC 4 cut(s) 37, 90, 162, 193
Eam1104I CTCTTC 1 cut(s) 152
EarI CTCTTC 1 cut(s) 152
Ecl136II GAGCTC 1 cut(s) 250
Eco24I GRGCYC 1 cut(s) 252
Eco53kI GAGCTC 1 cut(s) 250
Eco57I CTGAAG 1 cut(s) 339
Eco88I CYCGRG 1 cut(s) 245
EcoICRI GAGCTC 1 cut(s) 250
EcoRII CCWGG 1 cut(s) 61
EcoT38I GRGCYC 1 cut(s) 252
FaeI CATG 2 cut(s) 83, 131
FaiI YATR 6 cut(s) 11, 21, 81, 129, 134, 285
FalI AAGNNNNNCTT 2 cut(s) 294, 326
FaqI GGGAC 2 cut(s) 37, 44
FatI CATG 2 cut(s) 79, 127
FriOI GRGCYC 1 cut(s) 252
GsaI CCCAGC 1 cut(s) 258
HapII CCGG 1 cut(s) 231
HgaI GACGC 1 cut(s) 375
Hin1II CATG 2 cut(s) 83, 131
HinfI GANTC 2 cut(s) 74, 350
HpaII CCGG 1 cut(s) 231
Hpy166II GTNNAC 1 cut(s) 101
Hpy188I TCNGA 3 cut(s) 90, 349, 368
Hpy8I GTNNAC 1 cut(s) 101
Hpy99I CGWCG 1 cut(s) 282
HpyAV CCTTC 1 cut(s) 174
HpyCH4V TGCA 6 cut(s) 4, 85, 106, 215, 236, 308
HpyF10VI GCNNNNNNNGC 3 cut(s) 112, 206, 289
HpyF3I CTNAG 1 cut(s) 293
Hsp92II CATG 2 cut(s) 83, 131
Kzo9I GATC 4 cut(s) 37, 90, 162, 193
LmnI GCTCC 1 cut(s) 255
LpnPI CCDG 6 cut(s) 48, 75, 244, 268, 283, 330
LweI GCATC 3 cut(s) 94, 102, 202
MaeIII GTNAC 1 cut(s) 387
MalI GATC 4 cut(s) 39, 92, 164, 195
MboI GATC 4 cut(s) 37, 90, 162, 193
MboII GAAGA 3 cut(s) 169, 232, 332
MflI RGATCY 1 cut(s) 37
MhlI GDGCHC 1 cut(s) 252
MluCI AATT 5 cut(s) 32, 51, 149, 356, 377
MluI ACGCGT 1 cut(s) 384
MnlI CCTC 2 cut(s) 153, 254
MseI TTAA 1 cut(s) 42
MspCI CTTAAG 1 cut(s) 41
MspI CCGG 1 cut(s) 231
MspR9I CCNGG 1 cut(s) 63
MvaI CCWGG 1 cut(s) 63
MvnI CGCG 1 cut(s) 386
MwoI GCNNNNNNNGC 3 cut(s) 112, 206, 289
NdeII GATC 4 cut(s) 37, 90, 162, 193
NlaIII CATG 2 cut(s) 83, 131
NmuCI GTSAC 1 cut(s) 387
PaeR7I CTCGAG 1 cut(s) 245
PfeI GAWTC 2 cut(s) 74, 350
Psp124BI GAGCTC 1 cut(s) 252
Psp6I CCWGG 1 cut(s) 61
PspFI CCCAGC 1 cut(s) 254
PspGI CCWGG 1 cut(s) 61
PspXI VCTCGAGB 1 cut(s) 245
PstI CTGCAG 1 cut(s) 108
PsuI RGATCY 1 cut(s) 37
RsaI GTAC 1 cut(s) 220
RsaNI GTAC 1 cut(s) 219
SacI GAGCTC 1 cut(s) 252
SaqAI TTAA 1 cut(s) 42
Sau3AI GATC 4 cut(s) 37, 90, 162, 193
ScrFI CCNGG 1 cut(s) 63
SduI GDGCHC 1 cut(s) 252
SetI ASST 6 cut(s) 19, 175, 202, 227, 252, 260
SfaNI GCATC 3 cut(s) 94, 102, 202
SfcI CTRYAG 1 cut(s) 104
Sfr274I CTCGAG 1 cut(s) 245
SlaI CTCGAG 1 cut(s) 245
SmlI CTYRAG 2 cut(s) 41, 245
SmoI CTYRAG 2 cut(s) 41, 245
Sse9I AATT 5 cut(s) 32, 51, 149, 356, 377
SstI GAGCTC 1 cut(s) 252
StyD4I CCNGG 1 cut(s) 61
TaqI TCGA 2 cut(s) 196, 246
TasI AATT 5 cut(s) 32, 51, 149, 356, 377
TatI WGTACW 1 cut(s) 218
TfiI GAWTC 2 cut(s) 74, 350
Tru1I TTAA 1 cut(s) 42
Tru9I TTAA 1 cut(s) 42
TseFI GTSAC 1 cut(s) 387
Tsp45I GTSAC 1 cut(s) 387
TspDTI ATGAA 2 cut(s) 162, 339
TspGWI ACGGA 1 cut(s) 331
Vha464I CTTAAG 1 cut(s) 41
XapI RAATTY 2 cut(s) 149, 356
XhoI CTCGAG 1 cut(s) 245
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.