Rw2G004150

hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0012556
Physical Location & Seq
Forward (+)
3943374 .. 3948711
5338 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G004150.1

Sequence Viewer

Length: 930 bp
ATGGAGGAGAGGGGGGCTTTGAAATGGAGGAAGAACAAGGATGTTGTTCTGTGGTTTGGGTTGTCTCTGTTTCTGCTTTTTCCTCTTGCTTTGTCACTGAACAGTGAAGGAATTTATCTTACAATAATCCTTCTGGAGTTCTCGGTTTCAAAGCTTAAGAACCTTGAGGTTTTGAATTTGAAGAACAATCAGTTGACTGGTCCTATCGCTACAACTCTTACCCAAATTCCAAACCTTAGAACTCTGTTTACAGCTAAGCTTCATTTCAGCGTTACTAGTATCTGCCAATCAAAGGATATGGTAGGGTATGGGACTAAAGAATTAGATCTTAAGGGAAAAATTGTAGTCCCTGGATTTATTGATTCCCATGTGCATCTGATCTTTGGTGGACTGCAGATGATGCGTGTAGAACTACATGGCATAAACTCAAAAGATGAATTTGTGAAGAGGATCAAAGAAGCTGTGAGAAGGGTCTTTACTGATCGAGCTTATTCAGCAGATGCAAGTACAAGAATGACTCAAACACCTGCTAAGGAAACCCAGTCGTTGTCGGATGTGTATGGGTCTTCGTCTGGGGCTAGGGCAGCTATAGATCATGTGATAGAGCTCCCTAAATCCACAGATATGATAAAGGTGAAAGTGATGCAAAATATGGTCACCTCTGCTGATGATATATATGAGATGGAAATAGAAGTCCACCCCATGCAGACACTGAAAAAGATTAGGGAGAAGTTGGCGACAACAGAAAATTTGAGAGGCTGGCCTCTCCCGACAAATGGATATTCATTTCGGCGTCAACCTCTCCCCGGCAACCATGATGACAACGGTGAAGACTTGCTCGAGGGTGGGGGGAGAACCTTCCGAAAACTCGGAATAAGAAATGGTGATACTATCCTGGTGCCCTGGTGGTACCATCCAGTTTCCAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Amidohydro_3

Protein Analysis

309

Amino Acids

34.87

Weight (kDa)

9.23

Isoelectric Point (pI)

22.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Amidohydro_3 PF07969 107 - 157 2.5e-10 Amidohydrolase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 535
Acc36I ACCTGC 1 cut(s) 535
Acc65I GGTACC 1 cut(s) 909
AccB1I GGYRCC 2 cut(s) 898, 909
AclWI GGATC 1 cut(s) 458
AcsI RAATTY 5 cut(s) 111, 175, 225, 437, 748
AcyI GRCGYC 1 cut(s) 793
AfaI GTAC 2 cut(s) 508, 911
AfiI CCNNNNNNNGG 3 cut(s) 292, 776, 806
AflII CTTAAG 2 cut(s) 155, 329
AgsI TTSAA 4 cut(s) 22, 150, 175, 181
AhlI ACTAGT 1 cut(s) 275
AjnI CCWGG 3 cut(s) 349, 894, 902
AluBI AGCT 7 cut(s) 154, 254, 259, 461, 488, 587, 607
AluI AGCT 7 cut(s) 154, 254, 259, 461, 488, 587, 607
Alw21I GWGCWC 1 cut(s) 609
Alw26I GTCTC 1 cut(s) 69
AlwI GGATC 1 cut(s) 458
AlwNI CAGNNNCTG 1 cut(s) 712
Ama87I CYCGRG 1 cut(s) 839
AoxI GGCC 1 cut(s) 761
ApeKI GCWGC 1 cut(s) 584
ApoI RAATTY 5 cut(s) 111, 175, 225, 437, 748
Asp718I GGTACC 1 cut(s) 909
AspS9I GGNCC 1 cut(s) 200
AsuC2I CCSGG 1 cut(s) 807
AsuHPI GGTGA 4 cut(s) 646, 649, 839, 896
AvaI CYCGRG 1 cut(s) 839
AvaII GGWCC 1 cut(s) 200
BaeGI GKGCMC 1 cut(s) 903
BanI GGYRCC 2 cut(s) 898, 909
BanII GRGCYC 1 cut(s) 609
BarI GAAGNNNNNNTAC 2 cut(s) 460, 492
BbsI GAAGAC 2 cut(s) 558, 837
Bbv12I GWGCWC 1 cut(s) 609
BbvI GCAGC 1 cut(s) 596
BccI CCATC 2 cut(s) 676, 921
BciT130I CCWGG 3 cut(s) 351, 896, 904
BcnI CCSGG 1 cut(s) 807
BcoDI GTCTC 1 cut(s) 69
BcuI ACTAGT 1 cut(s) 275
BfaI CTAG 2 cut(s) 276, 579
BfmI CTRYAG 2 cut(s) 392, 588
BfrI CTTAAG 2 cut(s) 155, 329
BfuAI ACCTGC 1 cut(s) 535
BglII AGATCT 1 cut(s) 325
BisI GCNGC 1 cut(s) 585
BlpI GCTNAGC 1 cut(s) 255
BlsI GCNGC 1 cut(s) 586
Bme1390I CCNGG 4 cut(s) 351, 807, 896, 904
Bme18I GGWCC 1 cut(s) 200
BmeT110I CYCGRG 1 cut(s) 839
BmgT120I GGNCC 1 cut(s) 200
BmiI GGNNCC 2 cut(s) 900, 911
BmrFI CCNGG 4 cut(s) 351, 807, 896, 904
BmrI ACTGGG 1 cut(s) 535
BmsI GCATC 4 cut(s) 382, 390, 490, 633
BmuI ACTGGG 1 cut(s) 535
BpiI GAAGAC 2 cut(s) 558, 837
BpmI CTGGAG 1 cut(s) 155
Bpu10I CCTNAGC 1 cut(s) 531
Bpu1102I GCTNAGC 1 cut(s) 255
BpuEI CTTGAG 1 cut(s) 185
BpuMI CCSGG 1 cut(s) 807
BsaHI GRCGYC 1 cut(s) 793
BsaJI CCNNGG 3 cut(s) 349, 805, 902
Bsc4I CCNNNNNNNGG 3 cut(s) 292, 776, 806
Bse1I ACTGG 3 cut(s) 202, 541, 917
BseBI CCWGG 3 cut(s) 351, 896, 904
BseDI CCNNGG 3 cut(s) 349, 805, 902
BseGI GGATG 3 cut(s) 46, 559, 913
BseLI CCNNNNNNNGG 3 cut(s) 292, 776, 806
BseNI ACTGG 3 cut(s) 202, 541, 917
BseRI GAGGAG 1 cut(s) 20
BseSI GKGCMC 1 cut(s) 903
BseXI GCAGC 1 cut(s) 596
BshFI GGCC 1 cut(s) 763
BshNI GGYRCC 2 cut(s) 898, 909
BsiHKAI GWGCWC 1 cut(s) 609
BsiHKCI CYCGRG 1 cut(s) 839
BsiSI CCGG 1 cut(s) 807
BslFI GGGAC 2 cut(s) 325, 332
BslI CCNNNNNNNGG 3 cut(s) 292, 776, 806
BsmAI GTCTC 1 cut(s) 69
BsmFI GGGAC 2 cut(s) 325, 332
BsnI GGCC 1 cut(s) 763
BsoBI CYCGRG 1 cut(s) 839
Bsp1286I GDGCHC 2 cut(s) 609, 903
Bsp143I GATC 5 cut(s) 325, 378, 450, 481, 592
Bsp1720I GCTNAGC 1 cut(s) 255
BspANI GGCC 1 cut(s) 763
BspLI GGNNCC 2 cut(s) 900, 911
BspMAI CTGCAG 1 cut(s) 396
BspMI ACCTGC 1 cut(s) 535
BspPI GGATC 1 cut(s) 458
BspT107I GGYRCC 2 cut(s) 898, 909
BspTI CTTAAG 2 cut(s) 155, 329
BsrI ACTGG 3 cut(s) 202, 541, 917
BssECI CCNNGG 3 cut(s) 349, 805, 902
BssMI GATC 5 cut(s) 325, 378, 450, 481, 592
BssNI GRCGYC 1 cut(s) 793
Bst2UI CCWGG 3 cut(s) 351, 896, 904
Bst4CI ACNGT 2 cut(s) 104, 827
Bst6I CTCTTC 1 cut(s) 440
BstACI GRCGYC 1 cut(s) 793
BstAFI CTTAAG 2 cut(s) 155, 329
BstAPI GCANNNNNTGC 1 cut(s) 400
BstC8I GCNNGC 1 cut(s) 761
BstDEI CTNAG 3 cut(s) 236, 255, 531
BstEII GGTNACC 1 cut(s) 655
BstF5I GGATG 3 cut(s) 46, 559, 913
BstKTI GATC 5 cut(s) 328, 381, 453, 484, 595
BstMAI GTCTC 1 cut(s) 69
BstMBI GATC 5 cut(s) 325, 378, 450, 481, 592
BstMWI GCNNNNNNNGC 3 cut(s) 400, 494, 584
BstNI CCWGG 3 cut(s) 351, 896, 904
BstPI GGTNACC 1 cut(s) 655
BstSCI CCNGG 4 cut(s) 349, 805, 894, 902
BstSFI CTRYAG 2 cut(s) 392, 588
BstSLI GKGCMC 1 cut(s) 903
BstV1I GCAGC 1 cut(s) 596
BstV2I GAAGAC 2 cut(s) 558, 837
BstX2I RGATCY 1 cut(s) 325
BstYI RGATCY 1 cut(s) 325
BsuRI GGCC 1 cut(s) 763
BtsCI GGATG 3 cut(s) 46, 559, 913
BtsIMutI CAGTG 3 cut(s) 95, 109, 710
BveI ACCTGC 1 cut(s) 535
Cac8I GCNNGC 1 cut(s) 761
CaiI CAGNNNCTG 1 cut(s) 712
Cfr13I GGNCC 1 cut(s) 200
CseI GACGC 1 cut(s) 782
Csp6I GTAC 2 cut(s) 507, 910
CviAII CATG 5 cut(s) 368, 416, 596, 703, 815
CviQI GTAC 2 cut(s) 507, 910
DdeI CTNAG 3 cut(s) 236, 255, 531
DpnI GATC 5 cut(s) 327, 380, 452, 483, 594
DpnII GATC 5 cut(s) 325, 378, 450, 481, 592
Eam1104I CTCTTC 1 cut(s) 440
EarI CTCTTC 1 cut(s) 440
Ecl136II GAGCTC 1 cut(s) 607
Eco24I GRGCYC 1 cut(s) 609
Eco47I GGWCC 1 cut(s) 200
Eco53kI GAGCTC 1 cut(s) 607
Eco88I CYCGRG 1 cut(s) 839
Eco91I GGTNACC 1 cut(s) 655
EcoICRI GAGCTC 1 cut(s) 607
EcoO65I GGTNACC 1 cut(s) 655
EcoRII CCWGG 3 cut(s) 349, 894, 902
EcoT38I GRGCYC 1 cut(s) 609
FaeI CATG 5 cut(s) 371, 419, 599, 706, 818
FaqI GGGAC 2 cut(s) 325, 332
FatI CATG 5 cut(s) 367, 415, 595, 702, 814
Fnu4HI GCNGC 1 cut(s) 585
FokI GGATG 3 cut(s) 53, 566, 900
FriOI GRGCYC 1 cut(s) 609
Fsp4HI GCNGC 1 cut(s) 585
FspBI CTAG 2 cut(s) 276, 579
GluI GCNGC 1 cut(s) 585
GsuI CTGGAG 1 cut(s) 155
HaeIII GGCC 1 cut(s) 763
HapII CCGG 1 cut(s) 807
HgaI GACGC 1 cut(s) 782
Hin1I GRCGYC 1 cut(s) 793
Hin1II CATG 5 cut(s) 371, 419, 599, 706, 818
HincII GTYRAC 2 cut(s) 195, 797
HindII GTYRAC 2 cut(s) 195, 797
HindIII AAGCTT 2 cut(s) 152, 257
HinfI GANTC 2 cut(s) 362, 517
HpaII CCGG 1 cut(s) 807
HphI GGTGA 4 cut(s) 646, 649, 839, 896
Hpy166II GTNNAC 5 cut(s) 195, 249, 389, 697, 797
Hpy188I TCNGA 4 cut(s) 378, 553, 863, 872
Hpy188III TCNNGA 2 cut(s) 134, 769
Hpy8I GTNNAC 5 cut(s) 195, 249, 389, 697, 797
HpyAV CCTTC 4 cut(s) 101, 140, 462, 868
HpyCH4III ACNGT 2 cut(s) 104, 827
HpyCH4V TGCA 5 cut(s) 373, 394, 503, 646, 706
HpyF10VI GCNNNNNNNGC 3 cut(s) 400, 494, 584
HpyF3I CTNAG 3 cut(s) 236, 255, 531
Hsp92I GRCGYC 1 cut(s) 793
Hsp92II CATG 5 cut(s) 371, 419, 599, 706, 818
KpnI GGTACC 1 cut(s) 913
Kzo9I GATC 5 cut(s) 325, 378, 450, 481, 592
LmnI GCTCC 1 cut(s) 612
Lsp1109I GCAGC 1 cut(s) 596
LweI GCATC 4 cut(s) 382, 390, 490, 633
MaeI CTAG 2 cut(s) 276, 579
MaeIII GTNAC 3 cut(s) 93, 271, 655
MalI GATC 5 cut(s) 327, 380, 452, 483, 594
MboI GATC 5 cut(s) 325, 378, 450, 481, 592
MboII GAAGA 5 cut(s) 43, 193, 457, 558, 842
MflI RGATCY 1 cut(s) 325
MhlI GDGCHC 2 cut(s) 609, 903
MluCI AATT 7 cut(s) 111, 175, 225, 320, 339, 437, 748
MlyI GAGTC 1 cut(s) 511
MmeI TCCRAC 1 cut(s) 531
MseI TTAA 2 cut(s) 156, 330
MslI CAYNNNNRTG 1 cut(s) 623
MspCI CTTAAG 2 cut(s) 155, 329
MspI CCGG 1 cut(s) 807
MspR9I CCNGG 4 cut(s) 351, 807, 896, 904
MvaI CCWGG 3 cut(s) 351, 896, 904
MwoI GCNNNNNNNGC 3 cut(s) 400, 494, 584
NciI CCSGG 1 cut(s) 807
NdeII GATC 5 cut(s) 325, 378, 450, 481, 592
NlaIII CATG 5 cut(s) 371, 419, 599, 706, 818
NlaIV GGNNCC 2 cut(s) 900, 911
NmuCI GTSAC 2 cut(s) 93, 655
PaeR7I CTCGAG 1 cut(s) 839
PaqCI CACCTGC 1 cut(s) 535
PfeI GAWTC 1 cut(s) 362
PkrI GCNGC 1 cut(s) 586
PleI GAGTC 1 cut(s) 511
PpsI GAGTC 1 cut(s) 511
Psp124BI GAGCTC 1 cut(s) 609
Psp6I CCWGG 3 cut(s) 349, 894, 902
PspEI GGTNACC 1 cut(s) 655
PspGI CCWGG 3 cut(s) 349, 894, 902
PspN4I GGNNCC 2 cut(s) 900, 911
PspPI GGNCC 1 cut(s) 200
PspXI VCTCGAGB 1 cut(s) 839
PsrI GAACNNNNNNTAC 2 cut(s) 232, 264
PstI CTGCAG 1 cut(s) 396
PstNI CAGNNNCTG 1 cut(s) 712
PsuI RGATCY 1 cut(s) 325
RsaI GTAC 2 cut(s) 508, 911
RsaNI GTAC 2 cut(s) 507, 910
RseI CAYNNNNRTG 1 cut(s) 623
SacI GAGCTC 1 cut(s) 609
SaqAI TTAA 2 cut(s) 156, 330
SatI GCNGC 1 cut(s) 585
Sau3AI GATC 5 cut(s) 325, 378, 450, 481, 592
Sau96I GGNCC 1 cut(s) 200
SchI GAGTC 1 cut(s) 511
ScrFI CCNGG 4 cut(s) 351, 807, 896, 904
SduI GDGCHC 2 cut(s) 609, 903
SfaNI GCATC 4 cut(s) 382, 390, 490, 633
SfcI CTRYAG 2 cut(s) 392, 588
Sfr274I CTCGAG 1 cut(s) 839
SinI GGWCC 1 cut(s) 200
SlaI CTCGAG 1 cut(s) 839
SmiMI CAYNNNNRTG 1 cut(s) 623
SmlI CTYRAG 4 cut(s) 155, 164, 329, 839
SmoI CTYRAG 4 cut(s) 155, 164, 329, 839
SpeI ACTAGT 1 cut(s) 275
Sse9I AATT 7 cut(s) 111, 175, 225, 320, 339, 437, 748
SspMI CTAG 2 cut(s) 276, 579
SstI GAGCTC 1 cut(s) 609
StyD4I CCNGG 4 cut(s) 349, 805, 894, 902
TaaI ACNGT 2 cut(s) 104, 827
TaqI TCGA 2 cut(s) 484, 840
TasI AATT 7 cut(s) 111, 175, 225, 320, 339, 437, 748
TatI WGTACW 1 cut(s) 506
TfiI GAWTC 1 cut(s) 362
Tru1I TTAA 2 cut(s) 156, 330
Tru9I TTAA 2 cut(s) 156, 330
TscAI CASTG 3 cut(s) 102, 109, 717
TseFI GTSAC 2 cut(s) 93, 655
TseI GCWGC 1 cut(s) 584
Tsp45I GTSAC 2 cut(s) 93, 655
TspDTI ATGAA 3 cut(s) 251, 450, 774
TspRI CASTG 3 cut(s) 102, 109, 717
Vha464I CTTAAG 2 cut(s) 155, 329
VpaK11BI GGWCC 1 cut(s) 200
XapI RAATTY 5 cut(s) 111, 175, 225, 437, 748
XhoI CTCGAG 1 cut(s) 839
XspI CTAG 2 cut(s) 276, 579
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.