RchiOBHm_Chr6g0264271

50S ribosomal protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0003149
Physical Location & Seq
Forward (+)
19477073 .. 19477694
622 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 351 bp
ATGAATTTTGGAACATCACTATGCCAAAGATTGAATATAAAGCATCTGGTTACCAGTACTCCTAATGGGCGAGACTCTTTACCTAAGAATCTTGGAGTGAAGAAATTCGGTGGAGAGAGGGTGATACCTGGAAACATCATTGTTCGCCAGAGGGGCACCCGATTCCATCCTGGAAATTATGTTGGGTTAGGGAAAGATCACACTCTTTTTGCTCTGAAAGAAGGCACCGTAAAGTTTGAAAAGCACAAGCTCACTGGACGTAAGTGGGTGCATGTAGAGCCCAAAGACGGTCATGTGCTTCACCCTATATACGCGAATGCTGCAGCAGCAGAGACGGTGAAGACAGTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_L27

Protein Analysis

116

Amino Acids

12.85

Weight (kDa)

10.25

Isoelectric Point (pI)

12.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L27 PF01016 15 - 92 2.6e-33 Ribosomal L27 protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 155, 224
AccII CGCG 1 cut(s) 314
AcsI RAATTY 2 cut(s) 4, 104
AfaI GTAC 1 cut(s) 58
AfiI CCNNNNNNNGG 1 cut(s) 287
AgsI TTSAA 2 cut(s) 34, 239
AjnI CCWGG 2 cut(s) 127, 169
AluBI AGCT 1 cut(s) 250
AluI AGCT 1 cut(s) 250
Alw26I GTCTC 2 cut(s) 66, 326
ApeKI GCWGC 3 cut(s) 320, 323, 326
ApoI RAATTY 2 cut(s) 4, 104
Asp700I GAANNNNTTC 1 cut(s) 104
AsuHPI GGTGA 3 cut(s) 133, 293, 349
BaeGI GKGCMC 1 cut(s) 158
BanI GGYRCC 2 cut(s) 155, 224
BanII GRGCYC 1 cut(s) 282
BbsI GAAGAC 1 cut(s) 347
BbvI GCAGC 3 cut(s) 307, 335, 338
BccI CCATC 1 cut(s) 174
BciT130I CCWGG 2 cut(s) 129, 171
BcoDI GTCTC 2 cut(s) 66, 326
BfmI CTRYAG 1 cut(s) 321
BglI GCCNNNNNGGC 1 cut(s) 153
BisI GCNGC 3 cut(s) 321, 324, 327
BlsI GCNGC 3 cut(s) 322, 325, 328
BmcAI AGTACT 1 cut(s) 58
Bme1390I CCNGG 2 cut(s) 129, 171
BmiI GGNNCC 2 cut(s) 157, 226
BmrFI CCNGG 2 cut(s) 129, 171
BmsI GCATC 1 cut(s) 52
BpiI GAAGAC 1 cut(s) 347
BsaXI ACNNNNNCTCC 4 cut(s) 43, 73, 105, 135
Bsc4I CCNNNNNNNGG 1 cut(s) 287
Bse1I ACTGG 2 cut(s) 54, 259
BseBI CCWGG 2 cut(s) 129, 171
BseGI GGATG 1 cut(s) 166
BseLI CCNNNNNNNGG 1 cut(s) 287
BseNI ACTGG 2 cut(s) 54, 259
BseSI GKGCMC 1 cut(s) 158
BseXI GCAGC 3 cut(s) 307, 335, 338
Bsh1236I CGCG 1 cut(s) 314
BshNI GGYRCC 2 cut(s) 155, 224
BslI CCNNNNNNNGG 1 cut(s) 287
BsmAI GTCTC 2 cut(s) 66, 326
BsmBI CGTCTC 1 cut(s) 326
BsmI GAATGC 1 cut(s) 322
Bsp1286I GDGCHC 2 cut(s) 158, 282
Bsp143I GATC 1 cut(s) 196
BspFNI CGCG 1 cut(s) 314
BspLI GGNNCC 2 cut(s) 157, 226
BspMAI CTGCAG 1 cut(s) 325
BspT107I GGYRCC 2 cut(s) 155, 224
BsrI ACTGG 2 cut(s) 54, 259
BssMI GATC 1 cut(s) 196
Bst2UI CCWGG 2 cut(s) 129, 171
Bst4CI ACNGT 4 cut(s) 229, 290, 337, 346
BstDEI CTNAG 1 cut(s) 84
BstEII GGTNACC 1 cut(s) 49
BstF5I GGATG 1 cut(s) 166
BstFNI CGCG 1 cut(s) 314
BstKTI GATC 1 cut(s) 199
BstMAI GTCTC 2 cut(s) 66, 326
BstMBI GATC 1 cut(s) 196
BstMWI GCNNNNNNNGC 4 cut(s) 153, 277, 320, 326
BstNI CCWGG 2 cut(s) 129, 171
BstNSI RCATGY 1 cut(s) 275
BstPI GGTNACC 1 cut(s) 49
BstSCI CCNGG 2 cut(s) 127, 169
BstSFI CTRYAG 1 cut(s) 321
BstSLI GKGCMC 1 cut(s) 158
BstUI CGCG 1 cut(s) 314
BstV1I GCAGC 3 cut(s) 307, 335, 338
BstV2I GAAGAC 1 cut(s) 347
BtsCI GGATG 1 cut(s) 166
BtsIMutI CAGTG 1 cut(s) 252
Csp6I GTAC 1 cut(s) 57
CviAII CATG 2 cut(s) 272, 293
CviJI RGCY 2 cut(s) 250, 280
CviKI_1 RGCY 2 cut(s) 250, 280
CviQI GTAC 1 cut(s) 57
DdeI CTNAG 1 cut(s) 84
DpnI GATC 1 cut(s) 198
DpnII GATC 1 cut(s) 196
Eco24I GRGCYC 1 cut(s) 282
Eco91I GGTNACC 1 cut(s) 49
EcoO65I GGTNACC 1 cut(s) 49
EcoRII CCWGG 2 cut(s) 127, 169
EcoT38I GRGCYC 1 cut(s) 282
Esp3I CGTCTC 1 cut(s) 326
FaeI CATG 2 cut(s) 275, 296
FaiI YATR 7 cut(s) 22, 38, 180, 273, 294, 308, 310
FatI CATG 2 cut(s) 271, 292
Fnu4HI GCNGC 3 cut(s) 321, 324, 327
FokI GGATG 1 cut(s) 153
FriOI GRGCYC 1 cut(s) 282
Fsp4HI GCNGC 3 cut(s) 321, 324, 327
GluI GCNGC 3 cut(s) 321, 324, 327
Hin1II CATG 2 cut(s) 275, 296
HinfI GANTC 3 cut(s) 74, 88, 162
HphI GGTGA 3 cut(s) 133, 293, 349
Hpy188I TCNGA 2 cut(s) 216, 350
HpyAV CCTTC 1 cut(s) 215
HpyCH4III ACNGT 4 cut(s) 229, 290, 337, 346
HpyCH4IV ACGT 1 cut(s) 259
HpyCH4V TGCA 2 cut(s) 271, 323
HpyF10VI GCNNNNNNNGC 4 cut(s) 153, 277, 320, 326
HpyF3I CTNAG 1 cut(s) 84
HpySE526I ACGT 1 cut(s) 259
Hsp92II CATG 2 cut(s) 275, 296
Kzo9I GATC 1 cut(s) 196
LpnPI CCDG 8 cut(s) 32, 67, 114, 141, 156, 161, 183, 240
Lsp1109I GCAGC 3 cut(s) 307, 335, 338
LweI GCATC 1 cut(s) 52
MaeII ACGT 1 cut(s) 259
MaeIII GTNAC 1 cut(s) 49
MalI GATC 1 cut(s) 198
MboI GATC 1 cut(s) 196
MboII GAAGA 1 cut(s) 112
MhlI GDGCHC 2 cut(s) 158, 282
MluCI AATT 3 cut(s) 4, 104, 175
MlyI GAGTC 1 cut(s) 68
MnlI CCTC 2 cut(s) 111, 144
MroXI GAANNNNTTC 1 cut(s) 104
MslI CAYNNNNRTG 1 cut(s) 19
MspR9I CCNGG 2 cut(s) 129, 171
Mva1269I GAATGC 1 cut(s) 322
MvaI CCWGG 2 cut(s) 129, 171
MvnI CGCG 1 cut(s) 314
MwoI GCNNNNNNNGC 4 cut(s) 153, 277, 320, 326
NdeII GATC 1 cut(s) 196
NlaIII CATG 2 cut(s) 275, 296
NlaIV GGNNCC 2 cut(s) 157, 226
NspI RCATGY 1 cut(s) 275
PctI GAATGC 1 cut(s) 322
PdmI GAANNNNTTC 1 cut(s) 104
PfeI GAWTC 2 cut(s) 88, 162
PfoI TCCNGGA 1 cut(s) 169
PkrI GCNGC 3 cut(s) 322, 325, 328
PleI GAGTC 1 cut(s) 68
PpsI GAGTC 1 cut(s) 68
Psp6I CCWGG 2 cut(s) 127, 169
PspEI GGTNACC 1 cut(s) 49
PspGI CCWGG 2 cut(s) 127, 169
PspN4I GGNNCC 2 cut(s) 157, 226
PstI CTGCAG 1 cut(s) 325
RsaI GTAC 1 cut(s) 58
RsaNI GTAC 1 cut(s) 57
RseI CAYNNNNRTG 1 cut(s) 19
SatI GCNGC 3 cut(s) 321, 324, 327
Sau3AI GATC 1 cut(s) 196
ScaI AGTACT 1 cut(s) 58
SchI GAGTC 1 cut(s) 68
ScrFI CCNGG 2 cut(s) 129, 171
SduI GDGCHC 2 cut(s) 158, 282
SetI ASST 4 cut(s) 85, 130, 252, 262
SfaNI GCATC 1 cut(s) 52
SfcI CTRYAG 1 cut(s) 321
SmiMI CAYNNNNRTG 1 cut(s) 19
Sse9I AATT 3 cut(s) 4, 104, 175
StyD4I CCNGG 2 cut(s) 127, 169
TaaI ACNGT 4 cut(s) 229, 290, 337, 346
TaiI ACGT 1 cut(s) 262
TasI AATT 3 cut(s) 4, 104, 175
TatI WGTACW 1 cut(s) 56
TfiI GAWTC 2 cut(s) 88, 162
TscAI CASTG 1 cut(s) 259
TseI GCWGC 3 cut(s) 320, 323, 326
TspDTI ATGAA 1 cut(s) 17
TspRI CASTG 1 cut(s) 259
XapI RAATTY 2 cut(s) 4, 104
XceI RCATGY 1 cut(s) 275
XmnI GAANNNNTTC 1 cut(s) 104
ZrmI AGTACT 1 cut(s) 58
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.