RchiOBHm_Chr6g0310401

Neprosin

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0017074
Physical Location & Seq
Reverse (-)
67766738 .. 67767403
666 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 273 bp
ATGTATAACTGCAATTTTGATAAGAAGAAGATAACCGGTGAACTCTGGGTGGACTCGCACAAATCAACAGGTTGCTTTGATCTAACTTGCTCAGGATTTCTGCAAGCTCATAAAGACATAGCCCTTGGTATGCCCCTAGGACCTGTATCAAGTGGAGGAGGACCCCAATATCAAACCACTTTCAGCATCAACAAGGTTTGGACGCTTTTCTTTGGTCTATGCATTCTCTTCACATTGAATGTCTCATGTGACCATCCAATTTCAATGTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Neprosin Neprosin_AP

Protein Analysis

90

Amino Acids

9.96

Weight (kDa)

6.79

Isoelectric Point (pI)

40.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Neprosin PF03080 10 - 65 5.4e-13 Neprosin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0017074)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G25950 AT5G25960
fragaria_vesca FvH4_2g32291
malus_domestica MD08G1074000.v1.1
prunus_persica Prupe.1G415900_v2.0.a1
rosa_chinensis RchiOBHm_Chr6g0310401
rosa_laevigata RLG00000010485
rosa_roxburghii Rroxscaffold_7G00158560
rosa_rugosa Rorug06G0379900
rosa_samantha Rh6AG493900 Rh6BG503600 Rh6CG508800 Rh6DG494700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AgeI ACCGGT 1 cut(s) 35
AgsI TTSAA 2 cut(s) 238, 264
AluBI AGCT 1 cut(s) 107
AluI AGCT 1 cut(s) 107
Alw26I GTCTC 1 cut(s) 247
AsiGI ACCGGT 1 cut(s) 35
AspA2I CCTAGG 1 cut(s) 136
AspS9I GGNCC 2 cut(s) 140, 161
AsuHPI GGTGA 1 cut(s) 50
AvaII GGWCC 2 cut(s) 140, 161
AvrII CCTAGG 1 cut(s) 136
BccI CCATC 1 cut(s) 261
BcoDI GTCTC 1 cut(s) 247
BfaI CTAG 1 cut(s) 137
BlnI CCTAGG 1 cut(s) 136
Bme18I GGWCC 2 cut(s) 140, 161
BmgT120I GGNCC 2 cut(s) 140, 161
BmiI GGNNCC 1 cut(s) 163
BmsI GCATC 1 cut(s) 195
Bpu10I CCTNAGC 1 cut(s) 91
BsaJI CCNNGG 2 cut(s) 124, 136
BsaWI WCCGGW 1 cut(s) 35
Bse118I RCCGGY 1 cut(s) 35
BseDI CCNNGG 2 cut(s) 124, 136
BseGI GGATG 1 cut(s) 253
BseMII CTCAG 1 cut(s) 105
BseRI GAGGAG 1 cut(s) 171
BshTI ACCGGT 1 cut(s) 35
BsiSI CCGG 1 cut(s) 36
BsmAI GTCTC 1 cut(s) 247
BsmI GAATGC 1 cut(s) 222
Bsp143I GATC 1 cut(s) 79
BspCNI CTCAG 1 cut(s) 104
BspLI GGNNCC 1 cut(s) 163
BsrFI RCCGGY 1 cut(s) 35
BssAI RCCGGY 1 cut(s) 35
BssECI CCNNGG 2 cut(s) 124, 136
BssMI GATC 1 cut(s) 79
BssT1I CCWWGG 2 cut(s) 124, 136
Bst6I CTCTTC 1 cut(s) 233
BstC8I GCNNGC 1 cut(s) 105
BstDEI CTNAG 1 cut(s) 91
BstF5I GGATG 1 cut(s) 253
BstKTI GATC 1 cut(s) 82
BstMAI GTCTC 1 cut(s) 247
BstMBI GATC 1 cut(s) 79
BtsCI GGATG 1 cut(s) 253
Cac8I GCNNGC 1 cut(s) 105
Cfr10I RCCGGY 1 cut(s) 35
Cfr13I GGNCC 2 cut(s) 140, 161
CseI GACGC 1 cut(s) 211
CspAI ACCGGT 1 cut(s) 35
CviAII CATG 1 cut(s) 246
CviJI RGCY 2 cut(s) 107, 122
CviKI_1 RGCY 2 cut(s) 107, 122
DdeI CTNAG 1 cut(s) 91
DpnI GATC 1 cut(s) 81
DpnII GATC 1 cut(s) 79
Eam1104I CTCTTC 1 cut(s) 233
EarI CTCTTC 1 cut(s) 233
Eco130I CCWWGG 2 cut(s) 124, 136
Eco47I GGWCC 2 cut(s) 140, 161
EcoO109I RGGNCCY 2 cut(s) 140, 161
EcoT14I CCWWGG 2 cut(s) 124, 136
EcoT22I ATGCAT 1 cut(s) 224
ErhI CCWWGG 2 cut(s) 124, 136
FaeI CATG 1 cut(s) 249
FaiI YATR 6 cut(s) 6, 111, 119, 131, 220, 247
FatI CATG 1 cut(s) 245
FokI GGATG 1 cut(s) 240
FspBI CTAG 1 cut(s) 137
HapII CCGG 1 cut(s) 36
HgaI GACGC 1 cut(s) 211
Hin1II CATG 1 cut(s) 249
HinfI GANTC 1 cut(s) 53
HpaII CCGG 1 cut(s) 36
HphI GGTGA 1 cut(s) 50
Hpy166II GTNNAC 2 cut(s) 41, 52
Hpy188III TCNNGA 1 cut(s) 93
Hpy8I GTNNAC 2 cut(s) 41, 52
HpyCH4V TGCA 3 cut(s) 12, 103, 222
HpyF3I CTNAG 1 cut(s) 91
Hsp92II CATG 1 cut(s) 249
Kzo9I GATC 1 cut(s) 79
LpnPI CCDG 5 cut(s) 31, 49, 54, 78, 156
LweI GCATC 1 cut(s) 195
MaeI CTAG 1 cut(s) 137
MaeIII GTNAC 1 cut(s) 248
MalI GATC 1 cut(s) 81
MboI GATC 1 cut(s) 79
MboII GAAGA 3 cut(s) 37, 40, 220
MluCI AATT 2 cut(s) 13, 258
MlyI GAGTC 1 cut(s) 47
MnlI CCTC 2 cut(s) 149, 152
Mph1103I ATGCAT 1 cut(s) 224
MspI CCGG 1 cut(s) 36
Mva1269I GAATGC 1 cut(s) 222
NdeII GATC 1 cut(s) 79
NlaIII CATG 1 cut(s) 249
NlaIV GGNNCC 1 cut(s) 163
NmuCI GTSAC 1 cut(s) 248
NsiI ATGCAT 1 cut(s) 224
PctI GAATGC 1 cut(s) 222
PinAI ACCGGT 1 cut(s) 35
PleI GAGTC 1 cut(s) 47
PpsI GAGTC 1 cut(s) 47
PpuMI RGGWCCY 2 cut(s) 140, 161
Psp5II RGGWCCY 2 cut(s) 140, 161
PspN4I GGNNCC 1 cut(s) 163
PspPI GGNCC 2 cut(s) 140, 161
PspPPI RGGWCCY 2 cut(s) 140, 161
Sau3AI GATC 1 cut(s) 79
Sau96I GGNCC 2 cut(s) 140, 161
SchI GAGTC 1 cut(s) 47
SetI ASST 4 cut(s) 73, 109, 145, 198
SfaNI GCATC 1 cut(s) 195
SinI GGWCC 2 cut(s) 140, 161
Sse9I AATT 2 cut(s) 13, 258
SspMI CTAG 1 cut(s) 137
StyI CCWWGG 2 cut(s) 124, 136
TasI AATT 2 cut(s) 13, 258
TseFI GTSAC 1 cut(s) 248
Tsp45I GTSAC 1 cut(s) 248
VpaK11BI GGWCC 2 cut(s) 140, 161
XmaJI CCTAGG 1 cut(s) 136
XspI CTAG 1 cut(s) 137
Zsp2I ATGCAT 1 cut(s) 224
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.