RchiOBHm_Chr5g0003581

Tobamovirus multiplication protein 1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0012412
Physical Location & Seq
Reverse (-)
2239747 .. 2243886
4140 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 879 bp
ATGACGAGAATGCCACCGCCTTCGCTGCTGACGTTGGCGCCGATGGAGGCCGCGGCGTTCGTGATGGCCTGGTGGGACGATATCAACGAGTCGACTAAGTGGCAGGACGGCATCTTCTACTCTCTCTGCGCCGCCTACGCCCTCGTCTCCTCCGTCGCTCTGTTTCAACTAATAAGGATTGAATTGAGAGTGCCGGAGTACGGTTGGACGACGCAGAAGGTGTTTCATCTTATGAATTTCATTGTCAATGGAGTGCGCGCAATTGTCTTTGGATTTCACAAGCAAGTCTTTCTCTTGCAACCAAGGGTGTTGATATTGGTCTTGTTGGATCTTCCTGGACTGCTATTCTTCTCAACGTACACACTTCTTGTCCTATTTTGGGCAGAGATATATCACCAGGCGAGAAGTTTACCGACAGATAAGCTCAGGATTGTTTATATTTCAGTCAATGGTGGAATGTACTTCGTACAGATTTGCCTCTGGATATACCTCTGGTTTGATGACAACAGCGTTGTGGAGTTCATTGGAAAGATATTTATTGCAGCTGTGTCATTTGTGGCTGCACTAGGTTTCTTAATTTATGGAGGAAGGTTATTTTTCATGCTGAGACGTTTCCCAATTGAATCTAAAGGGAGAAGGAAGAAACTTCATGAGGTTGGATCTGTTACAGCCATATGTTTTACCTGCTTTCTCATAAGATGCTTTGTGGATGTGTTGTCCGCTTTTGATGATGATGCATCACTTGATGTTTTGGATCATCCTGTTTTAAATGCGTTCTACTACATGCTGGTTGAGATCCTGCCCTCAGCCTTAGTCCTCTTCATCTTGCGCAAATTACCCCCCAAGAGAGTATCAGCTCAGTATCATCCAATACGTTAG

Protein Analysis

292

Amino Acids

33.62

Weight (kDa)

8.76

Isoelectric Point (pI)

43.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
THH1_TOM1-3_dom PF06454 22 - 292 2.5e-141 THH1/TOM1/TOM3 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012412)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G21790
fragaria_vesca FvH4_3g02360 FvH4_3g02360
malus_domestica MD05G1343500.v1.1 MD10G1318600.v1.1
prunus_persica Prupe.4G023400_v2.0.a1
pyrus_communis pycom05g31290
rosa_chinensis RchiOBHm_Chr5g0003581
rosa_laevigata RLG00000031154
rosa_multiflora Rmu_sc0004279.1_g000004 Rmu_sc0015897.1_g000002 Rmu_sc0022447.1_g000003
rosa_roxburghii Rroxscaffold_1G00072380
rosa_rugosa Rorug04G0404700 Rorug04G0404800
rosa_samantha Rh5AG031600 Rh5BG030500 Rh5CG034200
rosa_wichuraiana Rw5G002940

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 830
Acc36I ACCTGC 1 cut(s) 692
AccB1I GGYRCC 1 cut(s) 37
AccI GTMKAC 1 cut(s) 92
AccII CGCG 2 cut(s) 53, 258
AciI CCGC 5 cut(s) 17, 51, 53, 132, 720
AclWI GGATC 4 cut(s) 336, 667, 762, 790
AcsI RAATTY 1 cut(s) 235
AcyI GRCGYC 1 cut(s) 38
AfaI GTAC 4 cut(s) 200, 359, 461, 468
AfiI CCNNNNNNNGG 2 cut(s) 200, 379
AgsI TTSAA 3 cut(s) 167, 182, 623
AjnI CCWGG 3 cut(s) 68, 334, 396
AluBI AGCT 3 cut(s) 424, 545, 857
AluI AGCT 3 cut(s) 424, 545, 857
Alw26I GTCTC 2 cut(s) 151, 601
AlwI GGATC 4 cut(s) 336, 667, 762, 790
AoxI GGCC 2 cut(s) 48, 66
ApeKI GCWGC 3 cut(s) 25, 542, 560
ApoI RAATTY 1 cut(s) 235
AspLEI GCGC 5 cut(s) 40, 131, 258, 260, 831
AsuHPI GGTGA 1 cut(s) 386
BanI GGYRCC 1 cut(s) 37
BbvCI CCTCAGC 1 cut(s) 805
BbvI GCAGC 3 cut(s) 12, 547, 554
BccI CCATC 2 cut(s) 37, 58
BceAI ACGGC 1 cut(s) 124
BciT130I CCWGG 3 cut(s) 70, 336, 398
BcoDI GTCTC 2 cut(s) 151, 601
BfaI CTAG 1 cut(s) 566
BfoI RGCGCY 1 cut(s) 41
BfuAI ACCTGC 1 cut(s) 692
BisI GCNGC 6 cut(s) 26, 51, 54, 132, 543, 561
BlsI GCNGC 6 cut(s) 27, 52, 55, 133, 544, 562
Bme1390I CCNGG 3 cut(s) 70, 336, 398
BmiI GGNNCC 1 cut(s) 39
BmrFI CCNGG 3 cut(s) 70, 336, 398
BmsI GCATC 4 cut(s) 120, 689, 724, 746
Bpu10I CCTNAGC 2 cut(s) 425, 805
BsaHI GRCGYC 1 cut(s) 38
BsaJI CCNNGG 2 cut(s) 51, 302
Bsc4I CCNNNNNNNGG 2 cut(s) 200, 379
BseBI CCWGG 3 cut(s) 70, 336, 398
BseDI CCNNGG 2 cut(s) 51, 302
BseGI GGATG 3 cut(s) 715, 757, 865
BseLI CCNNNNNNNGG 2 cut(s) 200, 379
BseMII CTCAG 4 cut(s) 439, 596, 819, 872
BsePI GCGCGC 1 cut(s) 256
BseRI GAGGAG 1 cut(s) 139
BseXI GCAGC 3 cut(s) 12, 547, 554
BsgI GTGCAG 1 cut(s) 546
Bsh1236I CGCG 2 cut(s) 53, 258
BshFI GGCC 2 cut(s) 50, 68
BshNI GGYRCC 1 cut(s) 37
BsiSI CCGG 1 cut(s) 194
BslFI GGGAC 1 cut(s) 89
BslI CCNNNNNNNGG 2 cut(s) 200, 379
BsmAI GTCTC 2 cut(s) 151, 601
BsmBI CGTCTC 2 cut(s) 151, 601
BsmFI GGGAC 1 cut(s) 89
BsmI GAATGC 1 cut(s) 15
BsnI GGCC 2 cut(s) 50, 68
Bsp143I GATC 4 cut(s) 328, 659, 754, 795
BspACI CCGC 5 cut(s) 17, 51, 53, 132, 720
BspANI GGCC 2 cut(s) 50, 68
BspCNI CTCAG 4 cut(s) 438, 597, 818, 871
BspFNI CGCG 2 cut(s) 53, 258
BspHI TCATGA 1 cut(s) 649
BspLI GGNNCC 1 cut(s) 39
BspMI ACCTGC 1 cut(s) 692
BspPI GGATC 4 cut(s) 336, 667, 762, 790
BspT107I GGYRCC 1 cut(s) 37
BssECI CCNNGG 2 cut(s) 51, 302
BssHII GCGCGC 1 cut(s) 256
BssMI GATC 4 cut(s) 328, 659, 754, 795
BssNI GRCGYC 1 cut(s) 38
BssT1I CCWWGG 1 cut(s) 302
Bst2UI CCWGG 3 cut(s) 70, 336, 398
Bst4CI ACNGT 1 cut(s) 203
Bst6I CTCTTC 1 cut(s) 824
BstACI GRCGYC 1 cut(s) 38
BstC8I GCNNGC 1 cut(s) 258
BstDEI CTNAG 6 cut(s) 96, 425, 605, 805, 811, 858
BstDSI CCRYGG 1 cut(s) 51
BstF5I GGATG 3 cut(s) 715, 757, 865
BstFNI CGCG 2 cut(s) 53, 258
BstH2I RGCGCY 1 cut(s) 41
BstHHI GCGC 5 cut(s) 40, 131, 258, 260, 831
BstKTI GATC 4 cut(s) 331, 662, 757, 798
BstMAI GTCTC 2 cut(s) 151, 601
BstMBI GATC 4 cut(s) 328, 659, 754, 795
BstMWI GCNNNNNNNGC 2 cut(s) 25, 137
BstNI CCWGG 3 cut(s) 70, 336, 398
BstNSI RCATGY 1 cut(s) 787
BstSCI CCNGG 3 cut(s) 68, 334, 396
BstUI CGCG 2 cut(s) 53, 258
BstV1I GCAGC 3 cut(s) 12, 547, 554
BstX2I RGATCY 3 cut(s) 328, 659, 795
BstYI RGATCY 3 cut(s) 328, 659, 795
BsuRI GGCC 2 cut(s) 50, 68
BtgI CCRYGG 1 cut(s) 51
BtsCI GGATG 3 cut(s) 715, 757, 865
BveI ACCTGC 1 cut(s) 692
Cac8I GCNNGC 1 cut(s) 258
CciI TCATGA 1 cut(s) 649
CfoI GCGC 5 cut(s) 40, 131, 258, 260, 831
Cfr42I CCGCGG 1 cut(s) 54
CseI GACGC 1 cut(s) 220
Csp6I GTAC 4 cut(s) 199, 358, 460, 467
CviAII CATG 3 cut(s) 601, 650, 784
CviJI RGCY 8 cut(s) 50, 68, 424, 545, 560, 671, 809, 857
CviKI_1 RGCY 8 cut(s) 50, 68, 424, 545, 560, 671, 809, 857
CviQI GTAC 4 cut(s) 199, 358, 460, 467
DdeI CTNAG 6 cut(s) 96, 425, 605, 805, 811, 858
DinI GGCGCC 1 cut(s) 39
DpnI GATC 4 cut(s) 330, 661, 756, 797
DpnII GATC 4 cut(s) 328, 659, 754, 795
DraI TTTAAA 1 cut(s) 768
Eam1104I CTCTTC 1 cut(s) 824
EarI CTCTTC 1 cut(s) 824
Eco130I CCWWGG 1 cut(s) 302
Eco32I GATATC 1 cut(s) 82
EcoRII CCWGG 3 cut(s) 68, 334, 396
EcoRV GATATC 1 cut(s) 82
EcoT14I CCWWGG 1 cut(s) 302
EcoT22I ATGCAT 1 cut(s) 739
EgeI GGCGCC 1 cut(s) 39
EheI GGCGCC 1 cut(s) 39
ErhI CCWWGG 1 cut(s) 302
Esp3I CGTCTC 2 cut(s) 151, 601
FaeI CATG 3 cut(s) 604, 653, 787
FalI AAGNNNNNCTT 2 cut(s) 272, 304
FaqI GGGAC 1 cut(s) 89
FatI CATG 3 cut(s) 600, 649, 783
FauNDI CATATG 1 cut(s) 674
FblI GTMKAC 1 cut(s) 92
Fnu4HI GCNGC 6 cut(s) 26, 51, 54, 132, 543, 561
FokI GGATG 3 cut(s) 722, 744, 852
Fsp4HI GCNGC 6 cut(s) 26, 51, 54, 132, 543, 561
FspBI CTAG 1 cut(s) 566
FspI TGCGCA 1 cut(s) 830
GlaI GCGC 5 cut(s) 39, 130, 257, 259, 830
GluI GCNGC 6 cut(s) 26, 51, 54, 132, 543, 561
HaeII RGCGCY 1 cut(s) 41
HaeIII GGCC 2 cut(s) 50, 68
HapII CCGG 1 cut(s) 194
HgaI GACGC 1 cut(s) 220
HhaI GCGC 5 cut(s) 40, 131, 258, 260, 831
Hin1I GRCGYC 1 cut(s) 38
Hin1II CATG 3 cut(s) 604, 653, 787
Hin6I GCGC 5 cut(s) 38, 129, 256, 258, 829
HinP1I GCGC 5 cut(s) 38, 129, 256, 258, 829
HincII GTYRAC 1 cut(s) 93
HindII GTYRAC 1 cut(s) 93
HinfI GANTC 2 cut(s) 89, 623
HpaII CCGG 1 cut(s) 194
HphI GGTGA 1 cut(s) 386
Hpy166II GTNNAC 3 cut(s) 93, 360, 410
Hpy188III TCNNGA 4 cut(s) 61, 427, 481, 650
Hpy8I GTNNAC 3 cut(s) 93, 360, 410
Hpy99I CGWCG 2 cut(s) 158, 214
HpyAV CCTTC 4 cut(s) 30, 211, 582, 630
HpyCH4III ACNGT 1 cut(s) 203
HpyCH4IV ACGT 4 cut(s) 32, 356, 610, 874
HpyCH4V TGCA 4 cut(s) 298, 542, 563, 737
HpyF10VI GCNNNNNNNGC 2 cut(s) 25, 137
HpyF3I CTNAG 6 cut(s) 96, 425, 605, 805, 811, 858
HpySE526I ACGT 4 cut(s) 32, 356, 610, 874
Hsp92I GRCGYC 1 cut(s) 38
Hsp92II CATG 3 cut(s) 604, 653, 787
HspAI GCGC 5 cut(s) 38, 129, 256, 258, 829
KasI GGCGCC 1 cut(s) 37
KspI CCGCGG 1 cut(s) 54
Kzo9I GATC 4 cut(s) 328, 659, 754, 795
Lsp1109I GCAGC 3 cut(s) 12, 547, 554
LweI GCATC 4 cut(s) 120, 689, 724, 746
MaeI CTAG 1 cut(s) 566
MaeII ACGT 4 cut(s) 32, 356, 610, 874
MaeIII GTNAC 1 cut(s) 664
MalI GATC 4 cut(s) 330, 661, 756, 797
MboI GATC 4 cut(s) 328, 659, 754, 795
MboII GAAGA 5 cut(s) 106, 323, 340, 652, 811
MfeI CAATTG 2 cut(s) 261, 618
MflI RGATCY 3 cut(s) 328, 659, 795
MluCI AATT 6 cut(s) 182, 235, 261, 576, 618, 833
Mly113I GGCGCC 1 cut(s) 38
MlyI GAGTC 1 cut(s) 98
MmeI TCCRAC 3 cut(s) 185, 306, 637
MnlI CCTC 9 cut(s) 40, 152, 160, 488, 500, 578, 646, 814, 827
Mph1103I ATGCAT 1 cut(s) 739
MseI TTAA 2 cut(s) 575, 767
MspA1I CMGCKG 2 cut(s) 53, 545
MspI CCGG 1 cut(s) 194
MspR9I CCNGG 3 cut(s) 70, 336, 398
MunI CAATTG 2 cut(s) 261, 618
Mva1269I GAATGC 1 cut(s) 15
MvaI CCWGG 3 cut(s) 70, 336, 398
MvnI CGCG 2 cut(s) 53, 258
MwoI GCNNNNNNNGC 2 cut(s) 25, 137
NarI GGCGCC 1 cut(s) 38
NdeI CATATG 1 cut(s) 674
NdeII GATC 4 cut(s) 328, 659, 754, 795
NlaIII CATG 3 cut(s) 604, 653, 787
NlaIV GGNNCC 1 cut(s) 39
NsbI TGCGCA 1 cut(s) 830
NsiI ATGCAT 1 cut(s) 739
NspI RCATGY 1 cut(s) 787
PagI TCATGA 1 cut(s) 649
PauI GCGCGC 1 cut(s) 256
PcsI WCGNNNNNNNCGW 4 cut(s) 29, 38, 84, 150
PctI GAATGC 1 cut(s) 15
PfeI GAWTC 1 cut(s) 623
PfoI TCCNGGA 1 cut(s) 334
PkrI GCNGC 6 cut(s) 27, 52, 55, 133, 544, 562
PleI GAGTC 1 cut(s) 97
PluTI GGCGCC 1 cut(s) 41
PpsI GAGTC 1 cut(s) 97
Psp6I CCWGG 3 cut(s) 68, 334, 396
PspGI CCWGG 3 cut(s) 68, 334, 396
PspN4I GGNNCC 1 cut(s) 39
PsuI RGATCY 3 cut(s) 328, 659, 795
PteI GCGCGC 1 cut(s) 256
PvuII CAGCTG 1 cut(s) 545
RsaI GTAC 4 cut(s) 200, 359, 461, 468
RsaNI GTAC 4 cut(s) 199, 358, 460, 467
SacII CCGCGG 1 cut(s) 54
SalI GTCGAC 1 cut(s) 91
SaqAI TTAA 2 cut(s) 575, 767
SatI GCNGC 6 cut(s) 26, 51, 54, 132, 543, 561
Sau3AI GATC 4 cut(s) 328, 659, 754, 795
SchI GAGTC 1 cut(s) 98
ScrFI CCNGG 3 cut(s) 70, 336, 398
SfaNI GCATC 4 cut(s) 120, 689, 724, 746
SfoI GGCGCC 1 cut(s) 39
Sfr303I CCGCGG 1 cut(s) 54
SgrBI CCGCGG 1 cut(s) 54
Sse9I AATT 6 cut(s) 182, 235, 261, 576, 618, 833
SsiI CCGC 5 cut(s) 17, 51, 53, 132, 720
SspDI GGCGCC 1 cut(s) 37
SspMI CTAG 1 cut(s) 566
StyD4I CCNGG 3 cut(s) 68, 334, 396
StyI CCWWGG 1 cut(s) 302
TaaI ACNGT 1 cut(s) 203
TaiI ACGT 4 cut(s) 35, 359, 613, 877
TaqI TCGA 1 cut(s) 92
TasI AATT 6 cut(s) 182, 235, 261, 576, 618, 833
TatI WGTACW 1 cut(s) 459
TauI GCSGC 3 cut(s) 53, 56, 134
TfiI GAWTC 1 cut(s) 623
Tru1I TTAA 2 cut(s) 575, 767
Tru9I TTAA 2 cut(s) 575, 767
TseI GCWGC 3 cut(s) 25, 542, 560
TspDTI ATGAA 7 cut(s) 215, 229, 248, 511, 589, 638, 811
TspGWI ACGGA 1 cut(s) 142
XapI RAATTY 1 cut(s) 235
XceI RCATGY 1 cut(s) 787
XmiI GTMKAC 1 cut(s) 92
XspI CTAG 1 cut(s) 566
Zsp2I ATGCAT 1 cut(s) 739
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.