RLG00000031154

Tobamovirus multiplication protein 1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0012412
Physical Location & Seq
Reverse (-)
2601572 .. 2605222
3651 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000031154

Sequence Viewer

Length: 888 bp
ATGACGAGAATGCCACCGCCTTCGCTGCTGACGTCGGCGCCGATGGAGGCCGTGGCGTTCGTGATGGCCTGGTGGGACGATATCAACGAGTCGACTAAGTGGCAGGACGGCATCTTCTACTCTCTCTGCGCGGCCTACGCCCTCGTCTCCTCCGTCGCTCTGTTTCAACTAATAAGGATTGAATTGAGAGTGCCGGAGTACGGTTGGACGACGCAGAAGGTGTTTCATCTTATGAATTTCATTGTCAATGGAGTGCGCGCAATTGTCTTTGGATTTCACAAGCAAATCTTTCTCTTGCAACCAAGGGTGTTGATATTGGTCTTATTGGATCTTCCTGGACTGCTATTCTTCTCAACGTACACACTTCTTGTCCTATTTTGGGCAGAGATATATCACCAGGCGAGAAGTTTACCGACAGATAAGCTCAGGATTGTTTATATTTCAGTCAATGGTGGAATGTACTTCGTGCAGATTTGCCTCTGGATATACCTCTGGTTTGATGACAACAGCATTGTGGAGTTCATTGGAAAGATATTTATTGCAGCTGTGTCATTTGTGGCTGCACTAGGTTTCTTAATTTATGGAGGAAGGTTATTTTTCATGCTGAGACGTTTCCCAATTGAATCTAAAGGGAGAAGGAAGAAACTTCATGAGGTTGGATCTGTTACAGCCATATGTTTCACCTGCTTTCTAATAAGATGCTTTGTGGATGTGTTGTCCGCTTTTGATGACGATGCATCACTTGATGTTTTGGATCATCCTGTTTTGAATGCGATCTACTACATGGTAGCTACCAAGCTTATTAACTTTCAAGCTGATTTTCTTGTCTTTTCTTTAACTTATTCAGTTTTTCCCCTTCAAAATTTTAACTATGTCCCCTGCAGCTAG

Protein Analysis

296

Amino Acids

33.93

Weight (kDa)

6.94

Isoelectric Point (pI)

36.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
THH1_TOM1-3_dom PF06454 22 - 264 3.7e-123 THH1/TOM1/TOM3 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012412)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G21790
fragaria_vesca FvH4_3g02360 FvH4_3g02360
malus_domestica MD05G1343500.v1.1 MD10G1318600.v1.1
prunus_persica Prupe.4G023400_v2.0.a1
pyrus_communis pycom05g31290
rosa_chinensis RchiOBHm_Chr5g0003581
rosa_laevigata RLG00000031154
rosa_multiflora Rmu_sc0004279.1_g000004 Rmu_sc0015897.1_g000002 Rmu_sc0022447.1_g000003
rosa_roxburghii Rroxscaffold_1G00072380
rosa_rugosa Rorug04G0404700 Rorug04G0404800
rosa_samantha Rh5AG031600 Rh5BG030500 Rh5CG034200
rosa_wichuraiana Rw5G002940

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 692
AatII GACGTC 1 cut(s) 35
Acc36I ACCTGC 1 cut(s) 692
AccB1I GGYRCC 1 cut(s) 37
AccI GTMKAC 1 cut(s) 92
AccII CGCG 2 cut(s) 131, 258
AciI CCGC 3 cut(s) 17, 131, 720
AclWI GGATC 3 cut(s) 336, 667, 762
AcsI RAATTY 2 cut(s) 235, 862
AcyI GRCGYC 2 cut(s) 32, 38
AfaI GTAC 3 cut(s) 200, 359, 461
AfiI CCNNNNNNNGG 2 cut(s) 200, 379
AgsI TTSAA 6 cut(s) 167, 182, 623, 769, 812, 860
AjnI CCWGG 3 cut(s) 68, 334, 396
AluBI AGCT 6 cut(s) 424, 545, 791, 799, 815, 885
AluI AGCT 6 cut(s) 424, 545, 791, 799, 815, 885
Alw26I GTCTC 2 cut(s) 151, 601
AlwI GGATC 3 cut(s) 336, 667, 762
AoxI GGCC 3 cut(s) 48, 66, 132
ApeKI GCWGC 4 cut(s) 25, 542, 560, 882
ApoI RAATTY 2 cut(s) 235, 862
AspLEI GCGC 4 cut(s) 40, 131, 258, 260
AsuHPI GGTGA 2 cut(s) 386, 673
BanI GGYRCC 1 cut(s) 37
BbvI GCAGC 3 cut(s) 12, 547, 554
BccI CCATC 2 cut(s) 37, 58
BceAI ACGGC 2 cut(s) 35, 124
BciT130I CCWGG 3 cut(s) 70, 336, 398
BcoDI GTCTC 2 cut(s) 151, 601
BfaI CTAG 2 cut(s) 566, 886
BfmI CTRYAG 1 cut(s) 880
BfoI RGCGCY 1 cut(s) 41
BfuAI ACCTGC 1 cut(s) 692
BisI GCNGC 5 cut(s) 26, 132, 543, 561, 883
BlsI GCNGC 5 cut(s) 27, 133, 544, 562, 884
Bme1390I CCNGG 3 cut(s) 70, 336, 398
BmiI GGNNCC 1 cut(s) 39
BmrFI CCNGG 3 cut(s) 70, 336, 398
BmsI GCATC 4 cut(s) 120, 689, 724, 746
Bpu10I CCTNAGC 1 cut(s) 425
BsaHI GRCGYC 2 cut(s) 32, 38
BsaJI CCNNGG 2 cut(s) 51, 302
Bsc4I CCNNNNNNNGG 2 cut(s) 200, 379
BseBI CCWGG 3 cut(s) 70, 336, 398
BseDI CCNNGG 2 cut(s) 51, 302
BseGI GGATG 2 cut(s) 715, 757
BseLI CCNNNNNNNGG 2 cut(s) 200, 379
BseMII CTCAG 2 cut(s) 439, 596
BsePI GCGCGC 1 cut(s) 256
BseRI GAGGAG 1 cut(s) 139
BseXI GCAGC 3 cut(s) 12, 547, 554
BsgI GTGCAG 2 cut(s) 488, 546
Bsh1236I CGCG 2 cut(s) 131, 258
BshFI GGCC 3 cut(s) 50, 68, 134
BshNI GGYRCC 1 cut(s) 37
BsiSI CCGG 1 cut(s) 194
BslFI GGGAC 2 cut(s) 89, 860
BslI CCNNNNNNNGG 2 cut(s) 200, 379
BsmAI GTCTC 2 cut(s) 151, 601
BsmBI CGTCTC 2 cut(s) 151, 601
BsmFI GGGAC 2 cut(s) 89, 860
BsmI GAATGC 2 cut(s) 15, 775
BsnI GGCC 3 cut(s) 50, 68, 134
Bsp143I GATC 4 cut(s) 328, 659, 754, 774
BspACI CCGC 3 cut(s) 17, 131, 720
BspANI GGCC 3 cut(s) 50, 68, 134
BspCNI CTCAG 2 cut(s) 438, 597
BspFNI CGCG 2 cut(s) 131, 258
BspHI TCATGA 1 cut(s) 649
BspLI GGNNCC 1 cut(s) 39
BspMAI CTGCAG 1 cut(s) 884
BspMI ACCTGC 1 cut(s) 692
BspPI GGATC 3 cut(s) 336, 667, 762
BspT107I GGYRCC 1 cut(s) 37
BssECI CCNNGG 2 cut(s) 51, 302
BssHII GCGCGC 1 cut(s) 256
BssMI GATC 4 cut(s) 328, 659, 754, 774
BssNI GRCGYC 2 cut(s) 32, 38
BssT1I CCWWGG 1 cut(s) 302
Bst2UI CCWGG 3 cut(s) 70, 336, 398
Bst4CI ACNGT 1 cut(s) 203
BstACI GRCGYC 2 cut(s) 32, 38
BstC8I GCNNGC 1 cut(s) 258
BstDEI CTNAG 3 cut(s) 96, 425, 605
BstDSI CCRYGG 1 cut(s) 51
BstF5I GGATG 2 cut(s) 715, 757
BstFNI CGCG 2 cut(s) 131, 258
BstH2I RGCGCY 1 cut(s) 41
BstHHI GCGC 4 cut(s) 40, 131, 258, 260
BstKTI GATC 4 cut(s) 331, 662, 757, 777
BstMAI GTCTC 2 cut(s) 151, 601
BstMBI GATC 4 cut(s) 328, 659, 754, 774
BstMWI GCNNNNNNNGC 2 cut(s) 25, 137
BstNI CCWGG 3 cut(s) 70, 336, 398
BstSCI CCNGG 3 cut(s) 68, 334, 396
BstSFI CTRYAG 1 cut(s) 880
BstUI CGCG 2 cut(s) 131, 258
BstV1I GCAGC 3 cut(s) 12, 547, 554
BstX2I RGATCY 2 cut(s) 328, 659
BstYI RGATCY 2 cut(s) 328, 659
BsuRI GGCC 3 cut(s) 50, 68, 134
BtgI CCRYGG 1 cut(s) 51
BtsCI GGATG 2 cut(s) 715, 757
BveI ACCTGC 1 cut(s) 692
Cac8I GCNNGC 1 cut(s) 258
CciI TCATGA 1 cut(s) 649
CfoI GCGC 4 cut(s) 40, 131, 258, 260
CseI GACGC 1 cut(s) 220
Csp6I GTAC 3 cut(s) 199, 358, 460
CviAII CATG 3 cut(s) 601, 650, 784
CviQI GTAC 3 cut(s) 199, 358, 460
DdeI CTNAG 3 cut(s) 96, 425, 605
DinI GGCGCC 1 cut(s) 39
DpnI GATC 4 cut(s) 330, 661, 756, 776
DpnII GATC 4 cut(s) 328, 659, 754, 774
Eco130I CCWWGG 1 cut(s) 302
Eco32I GATATC 1 cut(s) 82
EcoRII CCWGG 3 cut(s) 68, 334, 396
EcoRV GATATC 1 cut(s) 82
EcoT14I CCWWGG 1 cut(s) 302
EcoT22I ATGCAT 1 cut(s) 739
EgeI GGCGCC 1 cut(s) 39
EheI GGCGCC 1 cut(s) 39
ErhI CCWWGG 1 cut(s) 302
Esp3I CGTCTC 2 cut(s) 151, 601
FaeI CATG 3 cut(s) 604, 653, 787
FalI AAGNNNNNCTT 2 cut(s) 272, 304
FaqI GGGAC 2 cut(s) 89, 860
FatI CATG 3 cut(s) 600, 649, 783
FauNDI CATATG 1 cut(s) 674
FblI GTMKAC 1 cut(s) 92
Fnu4HI GCNGC 5 cut(s) 26, 132, 543, 561, 883
FokI GGATG 2 cut(s) 722, 744
Fsp4HI GCNGC 5 cut(s) 26, 132, 543, 561, 883
FspBI CTAG 2 cut(s) 566, 886
GlaI GCGC 4 cut(s) 39, 130, 257, 259
GluI GCNGC 5 cut(s) 26, 132, 543, 561, 883
HaeII RGCGCY 1 cut(s) 41
HaeIII GGCC 3 cut(s) 50, 68, 134
HapII CCGG 1 cut(s) 194
HgaI GACGC 1 cut(s) 220
HhaI GCGC 4 cut(s) 40, 131, 258, 260
Hin1I GRCGYC 2 cut(s) 32, 38
Hin1II CATG 3 cut(s) 604, 653, 787
Hin6I GCGC 4 cut(s) 38, 129, 256, 258
HinP1I GCGC 4 cut(s) 38, 129, 256, 258
HincII GTYRAC 1 cut(s) 93
HindII GTYRAC 1 cut(s) 93
HindIII AAGCTT 1 cut(s) 797
HinfI GANTC 2 cut(s) 89, 623
HpaII CCGG 1 cut(s) 194
HphI GGTGA 2 cut(s) 386, 673
Hpy166II GTNNAC 3 cut(s) 93, 360, 410
Hpy188III TCNNGA 4 cut(s) 61, 427, 481, 650
Hpy8I GTNNAC 3 cut(s) 93, 360, 410
Hpy99I CGWCG 3 cut(s) 37, 158, 214
HpyAV CCTTC 5 cut(s) 30, 211, 582, 630, 866
HpyCH4III ACNGT 1 cut(s) 203
HpyCH4IV ACGT 3 cut(s) 32, 356, 610
HpyCH4V TGCA 6 cut(s) 298, 469, 542, 563, 737, 882
HpyF10VI GCNNNNNNNGC 2 cut(s) 25, 137
HpyF3I CTNAG 3 cut(s) 96, 425, 605
HpySE526I ACGT 3 cut(s) 32, 356, 610
Hsp92I GRCGYC 2 cut(s) 32, 38
Hsp92II CATG 3 cut(s) 604, 653, 787
HspAI GCGC 4 cut(s) 38, 129, 256, 258
KasI GGCGCC 1 cut(s) 37
Kzo9I GATC 4 cut(s) 328, 659, 754, 774
Lsp1109I GCAGC 3 cut(s) 12, 547, 554
LweI GCATC 4 cut(s) 120, 689, 724, 746
MaeI CTAG 2 cut(s) 566, 886
MaeII ACGT 3 cut(s) 32, 356, 610
MaeIII GTNAC 1 cut(s) 664
MalI GATC 4 cut(s) 330, 661, 756, 776
MboI GATC 4 cut(s) 328, 659, 754, 774
MboII GAAGA 4 cut(s) 106, 323, 340, 652
MfeI CAATTG 2 cut(s) 261, 618
MflI RGATCY 2 cut(s) 328, 659
MluCI AATT 6 cut(s) 182, 235, 261, 576, 618, 862
Mly113I GGCGCC 1 cut(s) 38
MlyI GAGTC 1 cut(s) 98
MmeI TCCRAC 2 cut(s) 185, 637
MnlI CCTC 7 cut(s) 40, 152, 160, 488, 500, 578, 646
Mph1103I ATGCAT 1 cut(s) 739
MseI TTAA 4 cut(s) 575, 804, 836, 867
MspA1I CMGCKG 1 cut(s) 545
MspI CCGG 1 cut(s) 194
MspR9I CCNGG 3 cut(s) 70, 336, 398
MunI CAATTG 2 cut(s) 261, 618
Mva1269I GAATGC 2 cut(s) 15, 775
MvaI CCWGG 3 cut(s) 70, 336, 398
MvnI CGCG 2 cut(s) 131, 258
MwoI GCNNNNNNNGC 2 cut(s) 25, 137
NarI GGCGCC 1 cut(s) 38
NdeI CATATG 1 cut(s) 674
NdeII GATC 4 cut(s) 328, 659, 754, 774
NlaIII CATG 3 cut(s) 604, 653, 787
NlaIV GGNNCC 1 cut(s) 39
NsiI ATGCAT 1 cut(s) 739
PagI TCATGA 1 cut(s) 649
PaqCI CACCTGC 1 cut(s) 692
PauI GCGCGC 1 cut(s) 256
PcsI WCGNNNNNNNCGW 4 cut(s) 29, 38, 84, 150
PctI GAATGC 2 cut(s) 15, 775
PfeI GAWTC 1 cut(s) 623
PfoI TCCNGGA 1 cut(s) 334
PkrI GCNGC 5 cut(s) 27, 133, 544, 562, 884
PleI GAGTC 1 cut(s) 97
PluTI GGCGCC 1 cut(s) 41
PpsI GAGTC 1 cut(s) 97
Psp6I CCWGG 3 cut(s) 68, 334, 396
PspGI CCWGG 3 cut(s) 68, 334, 396
PspN4I GGNNCC 1 cut(s) 39
PstI CTGCAG 1 cut(s) 884
PsuI RGATCY 2 cut(s) 328, 659
PteI GCGCGC 1 cut(s) 256
PvuII CAGCTG 1 cut(s) 545
RsaI GTAC 3 cut(s) 200, 359, 461
RsaNI GTAC 3 cut(s) 199, 358, 460
SalI GTCGAC 1 cut(s) 91
SaqAI TTAA 4 cut(s) 575, 804, 836, 867
SatI GCNGC 5 cut(s) 26, 132, 543, 561, 883
Sau3AI GATC 4 cut(s) 328, 659, 754, 774
SchI GAGTC 1 cut(s) 98
ScrFI CCNGG 3 cut(s) 70, 336, 398
SfaNI GCATC 4 cut(s) 120, 689, 724, 746
SfcI CTRYAG 1 cut(s) 880
SfoI GGCGCC 1 cut(s) 39
Sse9I AATT 6 cut(s) 182, 235, 261, 576, 618, 862
SsiI CCGC 3 cut(s) 17, 131, 720
SspDI GGCGCC 1 cut(s) 37
SspMI CTAG 2 cut(s) 566, 886
StyD4I CCNGG 3 cut(s) 68, 334, 396
StyI CCWWGG 1 cut(s) 302
TaaI ACNGT 1 cut(s) 203
TaiI ACGT 3 cut(s) 35, 359, 613
TaqI TCGA 1 cut(s) 92
TasI AATT 6 cut(s) 182, 235, 261, 576, 618, 862
TatI WGTACW 1 cut(s) 459
TauI GCSGC 1 cut(s) 134
TfiI GAWTC 1 cut(s) 623
Tru1I TTAA 4 cut(s) 575, 804, 836, 867
Tru9I TTAA 4 cut(s) 575, 804, 836, 867
TseI GCWGC 4 cut(s) 25, 542, 560, 882
TspDTI ATGAA 6 cut(s) 215, 229, 248, 511, 589, 638
TspGWI ACGGA 1 cut(s) 142
XapI RAATTY 2 cut(s) 235, 862
XmiI GTMKAC 1 cut(s) 92
XspI CTAG 2 cut(s) 566, 886
ZraI GACGTC 1 cut(s) 33
Zsp2I ATGCAT 1 cut(s) 739
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.