Rh5BG030500

Tobamovirus multiplication protein 1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0012412
Physical Location & Seq
Reverse (-)
2144361 .. 2145739
1379 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG030500.1

Sequence Viewer

Length: 426 bp
ATGTGGATTTCTCTTCAGATTTGCCTCTGGATATACCTCTGGTTTGATGACAACAGCGTTGTGGAGTTCATTGGAAAGATATTTATTGCAGCTGTGTCATTTGTGGCTGCACTAGGTTTCTTAATCTATGGAGGAAGGTTATTTTTCATGCTGAGACGTTTCCCAATTGAATCTAAAGGGAGAAGGAAGAAACTTCATGAGGTTGGATCTGTTACAGCCATATGTTTTACCTGCTTTCTCATAAGATGCTTTGTGGATGTGTTGTCCGCTTTTGATGATGATGCATCACTTGATGTTTTGGATCATCCTGTTTTGAATGCGTTCTACTACATGCTGGTTGAGATCCTGCCCTCAGCCTTAGTCCTCTTCATCTTGCGCAAATTACCCCCCAAGAGAGTATCAGCTCAGTATCATCCAATACGTTAG

Protein Analysis

141

Amino Acids

16.3

Weight (kDa)

8.75

Isoelectric Point (pI)

49.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
THH1_TOM1-3_dom PF06454 5 - 141 4.8e-71 THH1/TOM1/TOM3 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012412)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G21790
fragaria_vesca FvH4_3g02360 FvH4_3g02360
malus_domestica MD05G1343500.v1.1 MD10G1318600.v1.1
prunus_persica Prupe.4G023400_v2.0.a1
pyrus_communis pycom05g31290
rosa_chinensis RchiOBHm_Chr5g0003581
rosa_laevigata RLG00000031154
rosa_multiflora Rmu_sc0004279.1_g000004 Rmu_sc0015897.1_g000002 Rmu_sc0022447.1_g000003
rosa_roxburghii Rroxscaffold_1G00072380
rosa_rugosa Rorug04G0404700 Rorug04G0404800
rosa_samantha Rh5AG031600 Rh5BG030500 Rh5CG034200
rosa_wichuraiana Rw5G002940

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 377
Acc36I ACCTGC 1 cut(s) 239
AciI CCGC 1 cut(s) 267
AclWI GGATC 3 cut(s) 214, 309, 337
AgsI TTSAA 2 cut(s) 170, 316
AluBI AGCT 2 cut(s) 92, 404
AluI AGCT 2 cut(s) 92, 404
Alw26I GTCTC 1 cut(s) 148
AlwI GGATC 3 cut(s) 214, 309, 337
ApeKI GCWGC 2 cut(s) 89, 107
Asp700I GAANNNNTTC 1 cut(s) 320
AspLEI GCGC 1 cut(s) 378
BbvCI CCTCAGC 1 cut(s) 352
BbvI GCAGC 2 cut(s) 94, 101
BcoDI GTCTC 1 cut(s) 148
BfaI CTAG 1 cut(s) 113
BfuAI ACCTGC 1 cut(s) 239
BisI GCNGC 2 cut(s) 90, 108
BlsI GCNGC 2 cut(s) 91, 109
BmsI GCATC 3 cut(s) 236, 271, 293
Bpu10I CCTNAGC 1 cut(s) 352
BseGI GGATG 3 cut(s) 262, 304, 412
BseMII CTCAG 3 cut(s) 143, 366, 419
BseXI GCAGC 2 cut(s) 94, 101
BsgI GTGCAG 1 cut(s) 93
BsmAI GTCTC 1 cut(s) 148
BsmBI CGTCTC 1 cut(s) 148
BsmI GAATGC 1 cut(s) 322
Bsp143I GATC 3 cut(s) 206, 301, 342
BspACI CCGC 1 cut(s) 267
BspCNI CTCAG 3 cut(s) 144, 365, 418
BspHI TCATGA 1 cut(s) 196
BspMI ACCTGC 1 cut(s) 239
BspPI GGATC 3 cut(s) 214, 309, 337
BssMI GATC 3 cut(s) 206, 301, 342
Bst6I CTCTTC 2 cut(s) 18, 371
BstDEI CTNAG 4 cut(s) 152, 352, 358, 405
BstF5I GGATG 3 cut(s) 262, 304, 412
BstHHI GCGC 1 cut(s) 378
BstKTI GATC 3 cut(s) 209, 304, 345
BstMAI GTCTC 1 cut(s) 148
BstMBI GATC 3 cut(s) 206, 301, 342
BstNSI RCATGY 1 cut(s) 334
BstV1I GCAGC 2 cut(s) 94, 101
BstX2I RGATCY 2 cut(s) 206, 342
BstYI RGATCY 2 cut(s) 206, 342
BtsCI GGATG 3 cut(s) 262, 304, 412
BveI ACCTGC 1 cut(s) 239
CciI TCATGA 1 cut(s) 196
CfoI GCGC 1 cut(s) 378
CviAII CATG 3 cut(s) 148, 197, 331
CviJI RGCY 5 cut(s) 92, 107, 218, 356, 404
CviKI_1 RGCY 5 cut(s) 92, 107, 218, 356, 404
DdeI CTNAG 4 cut(s) 152, 352, 358, 405
DpnI GATC 3 cut(s) 208, 303, 344
DpnII GATC 3 cut(s) 206, 301, 342
Eam1104I CTCTTC 2 cut(s) 18, 371
EarI CTCTTC 2 cut(s) 18, 371
EcoT22I ATGCAT 1 cut(s) 286
Esp3I CGTCTC 1 cut(s) 148
FaeI CATG 3 cut(s) 151, 200, 334
FaiI YATR 8 cut(s) 34, 129, 149, 198, 221, 223, 242, 332
FatI CATG 3 cut(s) 147, 196, 330
FauNDI CATATG 1 cut(s) 221
Fnu4HI GCNGC 2 cut(s) 90, 108
FokI GGATG 3 cut(s) 269, 291, 399
Fsp4HI GCNGC 2 cut(s) 90, 108
FspBI CTAG 1 cut(s) 113
FspI TGCGCA 1 cut(s) 377
GlaI GCGC 1 cut(s) 377
GluI GCNGC 2 cut(s) 90, 108
HhaI GCGC 1 cut(s) 378
Hin1II CATG 3 cut(s) 151, 200, 334
Hin6I GCGC 1 cut(s) 376
HinP1I GCGC 1 cut(s) 376
HinfI GANTC 1 cut(s) 170
Hpy188I TCNGA 1 cut(s) 18
Hpy188III TCNNGA 2 cut(s) 28, 197
HpyAV CCTTC 2 cut(s) 129, 177
HpyCH4IV ACGT 2 cut(s) 157, 421
HpyCH4V TGCA 3 cut(s) 89, 110, 284
HpyF3I CTNAG 4 cut(s) 152, 352, 358, 405
HpySE526I ACGT 2 cut(s) 157, 421
Hsp92II CATG 3 cut(s) 151, 200, 334
HspAI GCGC 1 cut(s) 376
Kzo9I GATC 3 cut(s) 206, 301, 342
LpnPI CCDG 6 cut(s) 13, 25, 244, 320, 321, 359
Lsp1109I GCAGC 2 cut(s) 94, 101
LweI GCATC 3 cut(s) 236, 271, 293
MaeI CTAG 1 cut(s) 113
MaeII ACGT 2 cut(s) 157, 421
MaeIII GTNAC 1 cut(s) 211
MalI GATC 3 cut(s) 208, 303, 344
MboI GATC 3 cut(s) 206, 301, 342
MboII GAAGA 3 cut(s) 5, 199, 358
MfeI CAATTG 1 cut(s) 165
MflI RGATCY 2 cut(s) 206, 342
MluCI AATT 2 cut(s) 165, 380
MmeI TCCRAC 1 cut(s) 184
MnlI CCTC 6 cut(s) 35, 47, 125, 193, 361, 374
Mph1103I ATGCAT 1 cut(s) 286
MroXI GAANNNNTTC 1 cut(s) 320
MseI TTAA 1 cut(s) 122
MspA1I CMGCKG 1 cut(s) 92
MunI CAATTG 1 cut(s) 165
Mva1269I GAATGC 1 cut(s) 322
NdeI CATATG 1 cut(s) 221
NdeII GATC 3 cut(s) 206, 301, 342
NlaIII CATG 3 cut(s) 151, 200, 334
NsbI TGCGCA 1 cut(s) 377
NsiI ATGCAT 1 cut(s) 286
NspI RCATGY 1 cut(s) 334
PagI TCATGA 1 cut(s) 196
PctI GAATGC 1 cut(s) 322
PdmI GAANNNNTTC 1 cut(s) 320
PfeI GAWTC 1 cut(s) 170
PkrI GCNGC 2 cut(s) 91, 109
PsuI RGATCY 2 cut(s) 206, 342
PvuII CAGCTG 1 cut(s) 92
SaqAI TTAA 1 cut(s) 122
SatI GCNGC 2 cut(s) 90, 108
Sau3AI GATC 3 cut(s) 206, 301, 342
SetI ASST 9 cut(s) 39, 94, 118, 140, 160, 204, 233, 406, 424
SfaNI GCATC 3 cut(s) 236, 271, 293
Sse9I AATT 2 cut(s) 165, 380
SsiI CCGC 1 cut(s) 267
SspMI CTAG 1 cut(s) 113
TaiI ACGT 2 cut(s) 160, 424
TasI AATT 2 cut(s) 165, 380
TfiI GAWTC 1 cut(s) 170
Tru1I TTAA 1 cut(s) 122
Tru9I TTAA 1 cut(s) 122
TseI GCWGC 2 cut(s) 89, 107
TspDTI ATGAA 4 cut(s) 58, 136, 185, 358
XceI RCATGY 1 cut(s) 334
XmnI GAANNNNTTC 1 cut(s) 320
XspI CTAG 1 cut(s) 113
Zsp2I ATGCAT 1 cut(s) 286
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.