RchiOBHm_Chr5g0027651

Bystin

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0008571
Physical Location & Seq
Forward (+)
21550673 .. 21554567
3895 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1389 bp
ATGCCGAAGAAGAGAGGGAGAGAGAGGCATCACAACCCGGAGCCATTTCTGGGCGACGACGATGGCGCCTCTGTGGCCTCCAAGAAGCGGACCAAGTCCAAGCAGCACCAGACCGACGAGACTCTGATTTCGTCGGGCATGAGCGCCAAGATTCTCAAGCAGGCTCAGCTTCAGCAGAGGGAGGTCGAGGACGACGAGAATCGAACCCAAAACCCCAACGCGTTATCGGACATCACCGAAGAGCCGCACGGGGTTGAATCTGATGACGACGATGAGGACGACCCCGATGAGTTCGGTGGGTTTCTTGACAATCGCGGCGAGGATGAAGCGTTTAAAGTGACGAACAGGATGACAAAGGAGATTGGGAAGGAGTATGAGAAAGTATTGGGTAAGGCGGCGGTGGAAGAGGATGAGAAATTACTGGATGCATTTCTTTCGAAGGATGCGGGGCGGCAGCTTACTCTTGCAGACCTGATTGTTGCAAGGATTAAAGAGAATGATTCTAATATCACTCCATCAAATGCCAATTCAGATATGAGACCTTTGCCTAAATTGGATGCAGACACCATAGAAATCTTCAAGGGGGTTGGCCAGTTTCTTAAAAAGTACACGGCTGGAAAGGTTCCCAAACCCTTTAAACACATCCCTTCAATGAATCAATGGGAAGATGTCCTATATTTAACTGAGCCTGAGAATTGGTCTCCCAATGCGATGTATCAAGCTACAAGAATTTTTGCTTCCAATTTGACTGCGAAGAAGGTAGAACGTTTCTACAAGCTTGTCTTGCTTCCACGTGTGAGAGAAGACATTCGGAAGAACAAGCGTTTGCATTTTGCCCTTTATCAGGCTTTGAAAAAGTCTCTTTACAAGCCTGCTGCCTTTTTCAAAGGAATATTGCTACCTTTGTGCGAGTCAGGGACGTGCAATCTAAGAGAAGCTGTCATAGTTGGGAGCGTCTTACAAAAGGTTTCCATTCCGCCACTTCATTCAAGTGTTGCAGTAATGAAGCTAGCAGACATGGAATACTGTGGTACAACGAGTTATTTTATAAAGCTTCTTTTGGAGAAAAAGTATGCTTTGCCATATCGTGTAATTGATGCTCTTGTTGGACATTTTATGAGATTTATGAATGAAACAAGGGTAATGCCAGTGATATGGCACCAATCACTTCTTGCATTTGTGGAAAGGTACAAAAATGATATACTGAACGAGGATAAAGATAATCTTAGAGTTCTTCTTCAAACTCAAAAGCACGATCTGGTAACTCGTGAAATAAGAAAGGAGCTAGATAATAGCCGCAGTCGTGGTGAGAAGGAGGATGACCTTATGTCAATTGATATCCTTTTATTTTCTTTTTTGTTTGCTTCGTGTTTCAATTGTTTCACTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Bystin

Protein Analysis

462

Amino Acids

52.91

Weight (kDa)

6.27

Isoelectric Point (pI)

36.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Bystin PF05291 151 - 438 1.8e-130 Bystin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1049
AccB1I GGYRCC 2 cut(s) 65, 1158
AccII CGCG 2 cut(s) 221, 315
AciI CCGC 9 cut(s) 88, 245, 315, 395, 398, 446, 451, 977, 1297
AclI AACGTT 1 cut(s) 766
AcoI YGGCCR 1 cut(s) 589
AcsI RAATTY 1 cut(s) 729
AcuI CTGAAG 1 cut(s) 155
AcvI CACGTG 1 cut(s) 794
AcyI GRCGYC 1 cut(s) 66
AfaI GTAC 3 cut(s) 608, 1033, 1190
AfiI CCNNNNNNNGG 3 cut(s) 50, 87, 844
AflIII ACRYGT 2 cut(s) 219, 793
AgsI TTSAA 8 cut(s) 257, 580, 651, 853, 886, 990, 1241, 1375
AhdI GACNNNNNGTC 1 cut(s) 1327
AjiI CACGTC 1 cut(s) 921
AluBI AGCT 8 cut(s) 169, 457, 722, 778, 938, 1009, 1054, 1285
AluI AGCT 8 cut(s) 169, 457, 722, 778, 938, 1009, 1054, 1285
Alw26I GTCTC 4 cut(s) 113, 532, 705, 864
AoxI GGCC 2 cut(s) 75, 589
ApeKI GCWGC 3 cut(s) 103, 454, 875
ApoI RAATTY 1 cut(s) 729
ArsI GACNNNNNNTTYG 2 cut(s) 112, 144
Asp700I GAANNNNTTC 1 cut(s) 807
AspLEI GCGC 2 cut(s) 68, 146
AspS9I GGNCC 1 cut(s) 90
AsuC2I CCSGG 1 cut(s) 38
AsuHPI GGTGA 2 cut(s) 226, 1319
AsuII TTCGAA 1 cut(s) 437
AsuNHI GCTAGC 1 cut(s) 1009
AvaII GGWCC 1 cut(s) 90
BalI TGGCCA 1 cut(s) 591
BanI GGYRCC 2 cut(s) 65, 1158
BauI CACGAG 1 cut(s) 1266
BbrPI CACGTG 1 cut(s) 794
BbsI GAAGAC 1 cut(s) 810
BbvI GCAGC 3 cut(s) 115, 466, 862
BccI CCATC 2 cut(s) 56, 523
BceAI ACGGC 1 cut(s) 627
BcgI CGANNNNNNTGC 2 cut(s) 417, 451
BcnI CCSGG 1 cut(s) 38
BcoDI GTCTC 4 cut(s) 113, 532, 705, 864
BfaI CTAG 2 cut(s) 1010, 1286
BfoI RGCGCY 2 cut(s) 69, 147
BglI GCCNNNNNGGC 1 cut(s) 74
BisI GCNGC 8 cut(s) 104, 245, 316, 396, 452, 455, 876, 1297
BlpI GCTNAGC 1 cut(s) 165
BlsI GCNGC 8 cut(s) 105, 246, 317, 397, 453, 456, 877, 1298
Bme1390I CCNGG 1 cut(s) 38
Bme18I GGWCC 1 cut(s) 90
BmeRI GACNNNNNGTC 1 cut(s) 1327
BmgBI CACGTC 1 cut(s) 921
BmgT120I GGNCC 1 cut(s) 90
BmiI GGNNCC 4 cut(s) 42, 67, 624, 1160
BmrFI CCNGG 1 cut(s) 38
BmsI GCATC 5 cut(s) 37, 415, 433, 547, 1087
BmtI GCTAGC 1 cut(s) 1013
BpiI GAAGAC 1 cut(s) 810
Bpu1102I GCTNAGC 1 cut(s) 165
Bpu14I TTCGAA 1 cut(s) 437
BpuEI CTTGAG 1 cut(s) 140
BpuMI CCSGG 1 cut(s) 38
BsaAI YACGTR 1 cut(s) 794
BsaHI GRCGYC 1 cut(s) 66
BsaI GGTCTC 2 cut(s) 532, 705
BsaXI ACNNNNNCTCC 2 cut(s) 1055, 1085
Bsc4I CCNNNNNNNGG 3 cut(s) 50, 87, 844
Bse1I ACTGG 3 cut(s) 426, 592, 1148
BseGI GGATG 8 cut(s) 328, 354, 415, 430, 448, 562, 642, 1324
BseLI CCNNNNNNNGG 3 cut(s) 50, 87, 844
BseMII CTCAG 3 cut(s) 179, 675, 681
BseNI ACTGG 3 cut(s) 426, 592, 1148
BseXI GCAGC 3 cut(s) 115, 466, 862
Bsh1236I CGCG 2 cut(s) 221, 315
BshFI GGCC 2 cut(s) 77, 591
BshNI GGYRCC 2 cut(s) 65, 1158
BsiSI CCGG 1 cut(s) 38
BslFI GGGAC 1 cut(s) 931
BslI CCNNNNNNNGG 3 cut(s) 50, 87, 844
BsmAI GTCTC 4 cut(s) 113, 532, 705, 864
BsmFI GGGAC 1 cut(s) 931
BsnI GGCC 2 cut(s) 77, 591
Bso31I GGTCTC 2 cut(s) 532, 705
Bsp119I TTCGAA 1 cut(s) 437
Bsp143I GATC 1 cut(s) 1255
Bsp1720I GCTNAGC 1 cut(s) 165
BspACI CCGC 9 cut(s) 88, 245, 315, 395, 398, 446, 451, 977, 1297
BspANI GGCC 2 cut(s) 77, 591
BspCNI CTCAG 3 cut(s) 178, 676, 682
BspFNI CGCG 2 cut(s) 221, 315
BspLI GGNNCC 4 cut(s) 42, 67, 624, 1160
BspOI GCTAGC 1 cut(s) 1013
BspQI GCTCTTC 1 cut(s) 234
BspT104I TTCGAA 1 cut(s) 437
BspT107I GGYRCC 2 cut(s) 65, 1158
BspTNI GGTCTC 2 cut(s) 532, 705
BsrI ACTGG 3 cut(s) 426, 592, 1148
BssMI GATC 1 cut(s) 1255
BssNI GRCGYC 1 cut(s) 66
BssSI CACGAG 1 cut(s) 1266
Bst2BI CACGAG 1 cut(s) 1266
Bst4CI ACNGT 1 cut(s) 1028
Bst6I CTCTTC 3 cut(s) 5, 234, 399
BstACI GRCGYC 1 cut(s) 66
BstBAI YACGTR 1 cut(s) 794
BstBI TTCGAA 1 cut(s) 437
BstC8I GCNNGC 3 cut(s) 162, 873, 1011
BstDEI CTNAG 5 cut(s) 165, 684, 690, 929, 1226
BstENI CCTNNNNNAGG 1 cut(s) 842
BstF5I GGATG 8 cut(s) 328, 354, 415, 430, 448, 562, 642, 1324
BstFNI CGCG 2 cut(s) 221, 315
BstH2I RGCGCY 2 cut(s) 69, 147
BstHHI GCGC 2 cut(s) 68, 146
BstKTI GATC 1 cut(s) 1258
BstMAI GTCTC 4 cut(s) 113, 532, 705, 864
BstMBI GATC 1 cut(s) 1255
BstMWI GCNNNNNNNGC 3 cut(s) 74, 166, 784
BstSCI CCNGG 1 cut(s) 36
BstUI CGCG 2 cut(s) 221, 315
BstV1I GCAGC 3 cut(s) 115, 466, 862
BstV2I GAAGAC 1 cut(s) 810
BstXI CCANNNNNNTGG 1 cut(s) 1155
BsuRI GGCC 2 cut(s) 77, 591
BtgZI GCGATG 1 cut(s) 725
BtrI CACGTC 1 cut(s) 921
BtsCI GGATG 8 cut(s) 328, 354, 415, 430, 448, 562, 642, 1324
BtsIMutI CAGTG 1 cut(s) 1155
Cac8I GCNNGC 3 cut(s) 162, 873, 1011
CfoI GCGC 2 cut(s) 68, 146
Cfr13I GGNCC 1 cut(s) 90
CseI GACGC 1 cut(s) 943
Csp6I GTAC 3 cut(s) 607, 1032, 1189
CviAII CATG 2 cut(s) 139, 1018
CviQI GTAC 3 cut(s) 607, 1032, 1189
DdeI CTNAG 5 cut(s) 165, 684, 690, 929, 1226
DinI GGCGCC 1 cut(s) 67
DpnI GATC 1 cut(s) 1257
DpnII GATC 1 cut(s) 1255
DraI TTTAAA 2 cut(s) 334, 637
DriI GACNNNNNGTC 1 cut(s) 1327
EaeI YGGCCR 1 cut(s) 589
Eam1104I CTCTTC 3 cut(s) 5, 234, 399
Eam1105I GACNNNNNGTC 1 cut(s) 1327
EarI CTCTTC 3 cut(s) 5, 234, 399
EciI GGCGGA 1 cut(s) 966
Eco31I GGTCTC 2 cut(s) 532, 705
Eco32I GATATC 1 cut(s) 1339
Eco47I GGWCC 1 cut(s) 90
Eco57I CTGAAG 1 cut(s) 155
Eco72I CACGTG 1 cut(s) 794
EcoNI CCTNNNNNAGG 1 cut(s) 842
EcoRV GATATC 1 cut(s) 1339
EcoT22I ATGCAT 1 cut(s) 430
EgeI GGCGCC 1 cut(s) 67
EheI GGCGCC 1 cut(s) 67
FaeI CATG 2 cut(s) 142, 1021
FalI AAGNNNNNCTT 4 cut(s) 767, 799, 1209, 1241
FaqI GGGAC 1 cut(s) 931
FatI CATG 2 cut(s) 138, 1017
FauI CCCGC 1 cut(s) 439
Fnu4HI GCNGC 8 cut(s) 104, 245, 316, 396, 452, 455, 876, 1297
FokI GGATG 8 cut(s) 335, 361, 422, 437, 455, 569, 629, 1331
Fsp4HI GCNGC 8 cut(s) 104, 245, 316, 396, 452, 455, 876, 1297
FspBI CTAG 2 cut(s) 1010, 1286
GlaI GCGC 2 cut(s) 67, 145
GluI GCNGC 8 cut(s) 104, 245, 316, 396, 452, 455, 876, 1297
HaeII RGCGCY 2 cut(s) 69, 147
HaeIII GGCC 2 cut(s) 77, 591
HapII CCGG 1 cut(s) 38
HgaI GACGC 1 cut(s) 943
HhaI GCGC 2 cut(s) 68, 146
Hin1I GRCGYC 1 cut(s) 66
Hin1II CATG 2 cut(s) 142, 1021
Hin6I GCGC 2 cut(s) 66, 144
HinP1I GCGC 2 cut(s) 66, 144
HindIII AAGCTT 2 cut(s) 776, 1052
HinfI GANTC 7 cut(s) 121, 151, 199, 257, 500, 655, 911
HpaII CCGG 1 cut(s) 38
HphI GGTGA 2 cut(s) 226, 1319
Hpy166II GTNNAC 1 cut(s) 609
Hpy188I TCNGA 5 cut(s) 126, 229, 262, 532, 813
Hpy188III TCNNGA 2 cut(s) 305, 1268
Hpy8I GTNNAC 1 cut(s) 609
Hpy99I CGWCG 6 cut(s) 59, 62, 119, 136, 197, 272
HpyAV CCTTC 5 cut(s) 361, 433, 657, 751, 1306
HpyCH4III ACNGT 1 cut(s) 1028
HpyCH4IV ACGT 3 cut(s) 766, 793, 920
HpyCH4V TGCA 8 cut(s) 428, 467, 482, 560, 829, 924, 998, 1175
HpyF10VI GCNNNNNNNGC 3 cut(s) 74, 166, 784
HpyF3I CTNAG 5 cut(s) 165, 684, 690, 929, 1226
HpySE526I ACGT 3 cut(s) 766, 793, 920
Hsp92I GRCGYC 1 cut(s) 66
Hsp92II CATG 2 cut(s) 142, 1021
HspAI GCGC 2 cut(s) 66, 144
KasI GGCGCC 1 cut(s) 65
Kzo9I GATC 1 cut(s) 1255
LguI GCTCTTC 1 cut(s) 234
LmnI GCTCC 3 cut(s) 40, 951, 1282
Lsp1109I GCAGC 3 cut(s) 115, 466, 862
LweI GCATC 5 cut(s) 37, 415, 433, 547, 1087
MaeI CTAG 2 cut(s) 1010, 1286
MaeII ACGT 3 cut(s) 766, 793, 920
MaeIII GTNAC 2 cut(s) 337, 1261
MalI GATC 1 cut(s) 1257
MboI GATC 1 cut(s) 1255
MfeI CAATTG 2 cut(s) 1332, 1375
MlsI TGGCCA 1 cut(s) 591
MluCI AATT 9 cut(s) 416, 526, 551, 694, 729, 742, 1092, 1332, 1375
MluI ACGCGT 1 cut(s) 219
MluNI TGGCCA 1 cut(s) 591
Mly113I GGCGCC 1 cut(s) 66
MlyI GAGTC 2 cut(s) 115, 920
MmeI TCCRAC 1 cut(s) 1087
Mox20I TGGCCA 1 cut(s) 591
Mph1103I ATGCAT 1 cut(s) 430
MroXI GAANNNNTTC 1 cut(s) 807
MscI TGGCCA 1 cut(s) 591
MseI TTAA 6 cut(s) 333, 489, 600, 636, 680, 1387
MslI CAYNNNNRTG 1 cut(s) 990
Msp20I TGGCCA 1 cut(s) 591
MspI CCGG 1 cut(s) 38
MspR9I CCNGG 1 cut(s) 38
MunI CAATTG 2 cut(s) 1332, 1375
MvnI CGCG 2 cut(s) 221, 315
MwoI GCNNNNNNNGC 3 cut(s) 74, 166, 784
NarI GGCGCC 1 cut(s) 66
NciI CCSGG 1 cut(s) 38
NdeII GATC 1 cut(s) 1255
NheI GCTAGC 1 cut(s) 1009
NlaIII CATG 2 cut(s) 142, 1021
NlaIV GGNNCC 4 cut(s) 42, 67, 624, 1160
NmuCI GTSAC 1 cut(s) 337
NsiI ATGCAT 1 cut(s) 430
NspV TTCGAA 1 cut(s) 437
PciSI GCTCTTC 1 cut(s) 234
PcsI WCGNNNNNNNCGW 2 cut(s) 192, 276
PdmI GAANNNNTTC 1 cut(s) 807
PfeI GAWTC 5 cut(s) 151, 199, 257, 500, 655
PflFI GACNNNGTC 1 cut(s) 94
PkrI GCNGC 8 cut(s) 105, 246, 317, 397, 453, 456, 877, 1298
PleI GAGTC 2 cut(s) 115, 919
PluTI GGCGCC 1 cut(s) 69
PmaCI CACGTG 1 cut(s) 794
PmlI CACGTG 1 cut(s) 794
PpsI GAGTC 2 cut(s) 115, 919
Ppu21I YACGTR 1 cut(s) 794
PsiI TTATAA 1 cut(s) 1049
Psp1406I AACGTT 1 cut(s) 766
PspCI CACGTG 1 cut(s) 794
PspN4I GGNNCC 4 cut(s) 42, 67, 624, 1160
PspPI GGNCC 1 cut(s) 90
PsyI GACNNNGTC 1 cut(s) 94
RsaI GTAC 3 cut(s) 608, 1033, 1190
RsaNI GTAC 3 cut(s) 607, 1032, 1189
RseI CAYNNNNRTG 1 cut(s) 990
SapI GCTCTTC 1 cut(s) 234
SaqAI TTAA 6 cut(s) 333, 489, 600, 636, 680, 1387
SatI GCNGC 8 cut(s) 104, 245, 316, 396, 452, 455, 876, 1297
Sau3AI GATC 1 cut(s) 1255
Sau96I GGNCC 1 cut(s) 90
SchI GAGTC 2 cut(s) 115, 920
ScrFI CCNGG 1 cut(s) 38
SfaNI GCATC 5 cut(s) 37, 415, 433, 547, 1087
SfoI GGCGCC 1 cut(s) 67
SfuI TTCGAA 1 cut(s) 437
SinI GGWCC 1 cut(s) 90
SmiMI CAYNNNNRTG 1 cut(s) 990
SmlI CTYRAG 1 cut(s) 155
SmoI CTYRAG 1 cut(s) 155
Sse9I AATT 9 cut(s) 416, 526, 551, 694, 729, 742, 1092, 1332, 1375
SsiI CCGC 9 cut(s) 88, 245, 315, 395, 398, 446, 451, 977, 1297
SspDI GGCGCC 1 cut(s) 65
SspI AATATT 1 cut(s) 894
SspMI CTAG 2 cut(s) 1010, 1286
StyD4I CCNGG 1 cut(s) 36
TaaI ACNGT 1 cut(s) 1028
TaiI ACGT 3 cut(s) 769, 796, 923
TaqI TCGA 3 cut(s) 186, 202, 437
TaqII GACCGA 1 cut(s) 128
TasI AATT 9 cut(s) 416, 526, 551, 694, 729, 742, 1092, 1332, 1375
TatI WGTACW 1 cut(s) 606
TauI GCSGC 5 cut(s) 247, 318, 398, 454, 1299
TfiI GAWTC 5 cut(s) 151, 199, 257, 500, 655
Tru1I TTAA 6 cut(s) 333, 489, 600, 636, 680, 1387
Tru9I TTAA 6 cut(s) 333, 489, 600, 636, 680, 1387
TscAI CASTG 1 cut(s) 1155
TseFI GTSAC 1 cut(s) 337
TseI GCWGC 3 cut(s) 103, 454, 875
Tsp45I GTSAC 1 cut(s) 337
TspDTI ATGAA 6 cut(s) 339, 668, 974, 1019, 1142, 1146
TspRI CASTG 1 cut(s) 1155
Tth111I GACNNNGTC 1 cut(s) 94
VpaK11BI GGWCC 1 cut(s) 90
XagI CCTNNNNNAGG 1 cut(s) 842
XapI RAATTY 1 cut(s) 729
XmnI GAANNNNTTC 1 cut(s) 807
XspI CTAG 2 cut(s) 1010, 1286
Zsp2I ATGCAT 1 cut(s) 430
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.