Basic Information

Type: gene

Biological Identity

Species

rosa_chinensis

Chromosome

Orthogroup ID

OG0012309

Physical Location & Seq

Genomic Coordinates Reverse (-)

82759188 .. 82764562

Gene Structure 5375 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3135 bp

ATGTTGCCTTCCAAGACTAAACAACTCTCCCAAATTTCCCACACAATTTCAAAGCTCAGAGCTTTTCATGGTGGTTCCTCTCCAAATGTCAAAAGCAATCTCAAAGAAAATGGCTTCAGAAACAGAGCTCATAGAACCACCCAAACCTCAGGTTCAGATATGGAGAAAAGCATATACTCAATTCTCACTATAGACCGATGGGAGTCACTGAACCACATGGAGTATAAGTTGGCTTCTCTTAGACCAGTGCATGGAAGATTGGCCTTGAAATTCTTCAACTGGGTCATTGAACAGCCTGGTTTGGAACTCAATCATCTCACTCATATACTCTCTGTTACAACTCATATACTTGCTAGAGCTAGAATGTATGACTCTGCCAGGTTAATTTTGGGGCATTTAGCACAACTGGGTGCTGACTCGAAATCTGTTTTTGGTGCTTTGATGGATACTTACCCTCTTTGCAATTCAAACCCTTCAGTATTTGACCTTTTGATTAGAGTTTACTTGAGGGAAGGGATGGTTGGTAATGCTGTAGAGACTTTTTATTTGATGGGTTTAAGGGGGTTTAGGCCGTCGGGGTATACTTGTAATATGATTCTAGCTTCGTTGGCAAAGGACCAGAGGGTTGCATCGGTGTGGTCTTTTTTCAAAGAAATGCTGGCTAATAATGTTTGCCCTGATGTTAGTACTTTTAATATATTGATTAGTGTTTTGTGTGTGGAAGGGAAGCTTAGTAAAGCTAGTTATCTTTTGAGGAAGATGGAAAAGAGTGGTTATGTTCCTAATGTAGTTACTTATAATACTTTGCTCAATTGGTACTGCAAAAAGGGGAGGTATAAAGCAGCTTTTGAGCTTATTGATCATATGGGTTCTAAGGGTATTGAAGCAGATGTATGTACATATAACATGCTTATTGAGGATTTGTGTAGAAACAGTAGTAGTGCGAAAGGTTATTTGCTGTTGAAGAAGATGAGAAGGAAGAAGTTATCTCCTAATCAGGTTACCTACAATATCCTTATTAATGGGTTTGTCAAGGAGGGGAAAATTGGTGTGGCCACCAAGGTTTTTGATGAAATGTCAATGTTTAATCTTTCACCAAATTATGTTACTTATAATGCTCTGATTGACGGGCTCTGTCAAAAGGGTAAGCTTGAAGAAGCTCTCAGACTCTTGGACATGATGGAAGCTGTGGGACTAAAACCTAATGAAGTCAGCTACGGTGCTGTTTTAAATGGGTTGTGCAAGCATGGTAAGTTTGATTTAGCAAGAAGTACTTTTCAGAGAATGCGATTGAATGGGATTGTTAGTGGTTGCATTATGTATACATCGATCATGGATGGGTTATGCAAAAATGGGTTGCTTGATGAATCTGTGCATTTGCTTAATATGATGGTCCAGGATGCTGGGAACCTTGGTGCTGCAGAGGATTTCATGCAGCATATGAGCAGCATGGGTCTGGTTGCCAATTCCATTTCTTTTGATTGTATGATAAATGGGTATGGAAACATGGGTGATGCCTTAGGAGCGTTTTCTATGTTTGATGAAATGATTAGATTAGGTCATCACCCTAGTCCATTTACTTATGGAAGTATACTGAAGGGGCTATGCAGGGGCGGTCATTTGGAGGTGGCAAAGAAATTTTTGAAGAAACTCCACAGCATTCCATCTGCTGTTGATACTGTTGTGTACAATACAATACTTTCTGAGACATGTAAGTCAGGGAATTTGCAAGAGGCTGTTGTCCTTCTTGATGAGATGGTTGAGAATAATGTCCTGCCTGATACTCATACGTACAGTAGCCTTCTTGCTGGGTTATGCAGGAAGGGAAAAGTGGTTGCTGCCATCCTATTGTTTGGGAGAGTTCTGGAAAAAGGAATTTTGTCACCAAATCCCATAATGTGCACCTGTCTAGTTGATGGCCTTTTCAAGATTGGGCAACCTAAGGCTGCTTCATATCTATTTGAAGAGATGGAGAACAAAGGTCTGTACTCAGATACTATTGCTCTTAATGTAATGATGGATGGATACTCAAGGATGGGAAATATGATGAAGGCAAATGATCTCTTTTCAACTATGGGGGGTCGAAGATTGTCCCCCAATTTGGCTTCATGTAACATTCTGTTGCATGGATACTCAAAGAAACAAGACTTATTGGCATGCTCTATGGTGTACAAAAACATGATCAGGGTCAAATTATTGCCTGACAAATTGACATGTCACTCTCTTATTCTTGGATTATGTGAGTCAGGTAAGGCGGATATTGGCCACAAAATGTTGCATAAGATGATTATGGAAGGTGCTGTACCTGATCTTTTAACATTCAATTTGCTTATTAGCAAGTATTCTGAGATTGGGAAGATAGGGATGGCATTTGAGCTAGTGAGTGTCATGAATCTGTTAGGAGTTTCCGCTAATACAGACACTCATGGAGCAATCTTAAATGGACTGTTCAGAATTTCAGCTTTCCGCGCATCCCGTAGTCTTCTGTATGAAATGTTGGCCAAAGGATTTACTCCCAAGTATACACATTATTTCACTATAGTTAATGGTATGTGTAGAGTGGGAGATATACAAGGAGCATTTGAGCTAAAAGATCAGATGGAAGCTCTTGGTGTCACTTCCTGTGACATTGCTGAGAGTGCTATGGTTAGAGGGCTTGCGAAATGTGGGAAGGTTGAAGAAGCGATGCTGGTTCTTGATCACATGCTTCGAATGCAACTTGTTCCAACTACTGCTACTTTTACTACTTTGATGCATAAGTTCTGCAAAGAAGCCAACCTTGCAGAGGCTCTAAGATTGAGGGGTGTAATGGAACATTGTGGTGTGCCACTCGATGTTGCTGCTTTTAATGTTCTCATTTCAGGCCTTTGTGCTAATGGTGATGTTGTGGCTGCATTTGAGCTGTACAAGGAGATGATACGAAGTGGTCTCTTGCCCAACACAACAACGTACACTCTTCTCCTTGCTGCTGTTTCCTCTGGTAATAATCATATTGAAGGTGAAGAGATTTTGGGAGATTTATTGGGAAAGGGGTTGATATCTGGAAATTTGGATGGAAGCACCTTAACACTGCATGACCAATTGAATGTTGCAATGGGAACATTAAACTCTTGGAGGTGCAATAAGTGGAAATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0006139 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009451 GO:0009987 GO:0016070 GO:0016787 GO:0016788 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044464 GO:0046483 GO:0071704 GO:0090304 GO:0090305 GO:0097159 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PPR PPR_1 PPR_2 PPR_3 PPR_long

Protein Analysis

1044

Amino Acids

115.19

Weight (kDa)

8.73

Isoelectric Point (pI)

31.03

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPR_2	PF13041	195 - 239	4.8e-10	PPR repeat family
PPR_long	PF17177	205 - 340	1.6e-11	Pentacotripeptide-repeat region of PRORP
PPR_3	PF13812	216 - 274	2.7e-11	Pentatricopeptide repeat domain
PPR_1	PF12854	223 - 255	1.8e-09	PPR repeat
PPR_2	PF13041	226 - 271	5.7e-13	PPR repeat family
PPR_3	PF13812	250 - 306	2.8e-11	Pentatricopeptide repeat domain
TPR_24	PF23276	251 - 358	3.7e-06	Fungal tetratrico peptide repeats
PPR_1	PF12854	258 - 289	2.2e-11	PPR repeat
PPR_2	PF13041	261 - 310	6.9e-19	PPR repeat family
PPR	PF01535	264 - 294	1.7e-07	PPR repeat
PPR_3	PF13812	285 - 345	5e-09	Pentatricopeptide repeat domain
PPR_2	PF13041	297 - 345	9.4e-13	PPR repeat family
PPR_long	PF17177	312 - 420	1.8e-09	Pentacotripeptide-repeat region of PRORP
PPR_1	PF12854	328 - 360	1.7e-10	PPR repeat
PPR_2	PF13041	331 - 379	2.7e-16	PPR repeat family
PPR	PF01535	334 - 361	3.9e-06	PPR repeat
PPR_3	PF13812	354 - 411	9.9e-10	Pentatricopeptide repeat domain
PPR_1	PF12854	363 - 395	4.9e-13	PPR repeat
PPR_2	PF13041	366 - 415	5.2e-17	PPR repeat family
PPR	PF01535	369 - 398	5.9e-09	PPR repeat
PPR_1	PF12854	398 - 430	1.2e-06	PPR repeat
PPR_2	PF13041	401 - 450	5.1e-09	PPR repeat family
PPR	PF01535	404 - 434	6.5e-06	PPR repeat
PPR_1	PF12854	433 - 465	3.8e-06	PPR repeat
TPR_24	PF23276	474 - 582	1.3e-07	Fungal tetratrico peptide repeats
PPR_2	PF13041	489 - 537	3.8e-11	PPR repeat family
PPR_2	PF13041	559 - 607	5.7e-16	PPR repeat family
PPR_1	PF12854	559 - 587	5.1e-07	PPR repeat
PPR_1	PF12854	590 - 618	2.6e-06	PPR repeat
PPR_2	PF13041	665 - 713	2.5e-09	PPR repeat family
PPR_2	PF13041	699 - 747	5.4e-07	PPR repeat family
PPR_2	PF13041	769 - 817	3e-06	PPR repeat family
PPR_long	PF17177	787 - 925	1.6e-07	Pentacotripeptide-repeat region of PRORP
PPR_2	PF13041	881 - 923	9e-08	PPR repeat family
TPR_24	PF23276	881 - 972	3.1e-07	Fungal tetratrico peptide repeats
PPR_2	PF13041	909 - 957	5e-09	PPR repeat family
PPR_1	PF12854	941 - 972	2.5e-07	PPR repeat
PPR_2	PF13041	945 - 990	1.1e-13	PPR repeat family
PPR	PF01535	948 - 977	2.5e-07	PPR repeat

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0012309)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G55840 AT5G55840 AT5G55840
fragaria_vesca	FvH4_3g42930
malus_domestica	MD03G1028500.v1.1
prunus_persica	Prupe.6G024500_v2.0.a1 Prupe.6G024500_v2.0.a1
pyrus_communis	pycom03g02310
rosa_chinensis	RchiOBHm_Chr5g0076971
rosa_laevigata	RLG00000036637
rosa_multiflora	Rmu_sc0004655.1_g000020
rosa_roxburghii	Rroxscaffold_1G00005180
rosa_rugosa	Rorug05G0449800 Rorug05G0449900
rosa_samantha	Rh5AG504500 Rh5BG526200 Rh5CG550100 Rh5DG538400
rosa_wichuraiana	Rw5G047180

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	798, 1113
AasI	GACNNNNNNGTC	1 cut(s)	1714
AccB7I	CCANNNNNTGG	1 cut(s)	251
AccI	GTMKAC	4 cut(s)	581, 1322, 1591, 2522
AccII	CGCG	1 cut(s)	2469
AciI	CCGC	4 cut(s)	1614, 2255, 2409, 2467
AcoI	YGGCCR	3 cut(s)	1053, 2263, 2499
AcsI	RAATTY	7 cut(s)	33, 269, 1637, 1723, 1875, 2454, 3046
AcuI	CTGAAG	3 cut(s)	100, 459, 1616
AfiI	CCNNNNNNNGG	3 cut(s)	251, 2101, 2785
AflIII	ACRYGT	2 cut(s)	1709, 2213
AjnI	CCWGG	3 cut(s)	295, 377, 1395
AjuI	GAANNNNNNNTTGG	2 cut(s)	504, 536
Alw21I	GWGCWC	2 cut(s)	130, 1904
Alw26I	GTCTC	3 cut(s)	530, 1700, 2933
Alw44I	GTGCAC	1 cut(s)	1900
AoxI	GGCC	7 cut(s)	261, 569, 1053, 1918, 2263, 2499, 2863
ApaLI	GTGCAC	1 cut(s)	1900
ApeKI	GCWGC	9 cut(s)	842, 1418, 1435, 1446, 1838, 1946, 2840, 2891, 2966
ApoI	RAATTY	7 cut(s)	33, 269, 1637, 1723, 1875, 2454, 3046
ArsI	GACNNNNNNTTYG	2 cut(s)	529, 561
AseI	ATTAAT	1 cut(s)	1020
Asp700I	GAANNNNTTC	1 cut(s)	272
AspLEI	GCGC	1 cut(s)	2471
AspS9I	GGNCC	2 cut(s)	616, 1393
AsuHPI	GGTGA	6 cut(s)	1086, 1523, 1556, 1875, 2891, 3011
AsuII	TTCGAA	1 cut(s)	2710
AvaII	GGWCC	2 cut(s)	616, 1393
AxyI	CCTNAGG	3 cut(s)	148, 1519, 1941
BaeGI	GKGCMC	1 cut(s)	1904
BaeI	ACNNNNGTAYC	2 cut(s)	1668, 1701
BalI	TGGCCA	3 cut(s)	1055, 2265, 2501
BanII	GRGCYC	2 cut(s)	130, 1134
BarI	GAAGNNNNNNTAC	2 cut(s)	2286, 2318
BbsI	GAAGAC	1 cut(s)	2474
Bbv12I	GWGCWC	2 cut(s)	130, 1904
BbvI	GCAGC	9 cut(s)	854, 1405, 1447, 1458, 1825, 1933, 2827, 2878, 2953
BceAI	ACGGC	1 cut(s)	556
BcgI	CGANNNNNNTGC	2 cut(s)	2822, 2856
BciT130I	CCWGG	3 cut(s)	297, 379, 1397
BciVI	GTATCC	3 cut(s)	439, 2018, 2123
BclI	TGATCA	3 cut(s)	859, 2181, 2698
BcoDI	GTCTC	3 cut(s)	530, 1700, 2933
BfaI	CTAG	7 cut(s)	354, 360, 599, 741, 1569, 1910, 2378
BfmI	CTRYAG	4 cut(s)	189, 531, 1419, 2538
BfuI	GTATCC	3 cut(s)	439, 2018, 2123
BisI	GCNGC	9 cut(s)	843, 1419, 1436, 1447, 1839, 1947, 2841, 2892, 2967
BlsI	GCNGC	9 cut(s)	844, 1420, 1437, 1448, 1840, 1948, 2842, 2893, 2968
BmcAI	AGTACT	2 cut(s)	688, 1273
Bme1390I	CCNGG	3 cut(s)	297, 379, 1397
Bme18I	GGWCC	2 cut(s)	616, 1393
BmgT120I	GGNCC	2 cut(s)	616, 1393
BmiI	GGNNCC	2 cut(s)	76, 1409
BmrFI	CCNGG	3 cut(s)	297, 379, 1397
BmrI	ACTGGG	2 cut(s)	289, 416
BmsI	GCATC	6 cut(s)	638, 1390, 1504, 2480, 2676, 2742
BmuI	ACTGGG	2 cut(s)	289, 416
BpiI	GAAGAC	1 cut(s)	2474
Bpu14I	TTCGAA	1 cut(s)	2710
BpuEI	CTTGAG	2 cut(s)	526, 2014
Bsa29I	ATCGAT	1 cut(s)	1328
BsaAI	YACGTR	1 cut(s)	1791
BsaI	GGTCTC	1 cut(s)	2933
BsaJI	CCNNGG	2 cut(s)	1059, 1411
BsaXI	ACNNNNNCTCC	2 cut(s)	212, 242
Bsc4I	CCNNNNNNNGG	3 cut(s)	251, 2101, 2785
Bse1I	ACTGG	3 cut(s)	245, 284, 411
Bse21I	CCTNAGG	3 cut(s)	148, 1519, 1941
Bse3DI	GCAATG	2 cut(s)	2628, 3099
BseBI	CCWGG	3 cut(s)	297, 379, 1397
BseCI	ATCGAT	1 cut(s)	1328
BseDI	CCNNGG	2 cut(s)	1059, 1411
BseGI	GGATG	9 cut(s)	522, 1342, 1405, 1842, 2026, 2040, 2370, 2471, 3058
BseLI	CCNNNNNNNGG	3 cut(s)	251, 2101, 2785
BseMI	GCAATG	2 cut(s)	2628, 3099
BseMII	CTCAG	7 cut(s)	70, 162, 1177, 1695, 2004, 2337, 2625
BseNI	ACTGG	3 cut(s)	245, 284, 411
BseSI	GKGCMC	1 cut(s)	1904
BseXI	GCAGC	9 cut(s)	854, 1405, 1447, 1458, 1825, 1933, 2827, 2878, 2953
BseYI	CCCAGC	2 cut(s)	1403, 1808
Bsh1236I	CGCG	1 cut(s)	2469
BshFI	GGCC	7 cut(s)	263, 571, 1055, 1920, 2265, 2501, 2865
BshVI	ATCGAT	1 cut(s)	1328
BsiHKAI	GWGCWC	2 cut(s)	130, 1904
BslFI	GGGAC	2 cut(s)	1206, 2077
BslI	CCNNNNNNNGG	3 cut(s)	251, 2101, 2785
BsmAI	GTCTC	3 cut(s)	530, 1700, 2933
BsmFI	GGGAC	2 cut(s)	1206, 2077
BsmI	GAATGC	3 cut(s)	1290, 1659, 2718
BsnI	GGCC	7 cut(s)	263, 571, 1055, 1920, 2265, 2501, 2865
Bso31I	GGTCTC	1 cut(s)	2933
Bsp119I	TTCGAA	1 cut(s)	2710
Bsp1286I	GDGCHC	3 cut(s)	130, 1134, 1904
Bsp1407I	TGTACA	4 cut(s)	896, 1686, 2169, 2904
Bsp143I	GATC	7 cut(s)	859, 1329, 2059, 2181, 2308, 2593, 2698
BspACI	CCGC	4 cut(s)	1614, 2255, 2409, 2467
BspANI	GGCC	7 cut(s)	263, 571, 1055, 1920, 2265, 2501, 2865
BspCNI	CTCAG	7 cut(s)	69, 161, 1176, 1696, 2003, 2338, 2626
BspDI	ATCGAT	1 cut(s)	1328
BspFNI	CGCG	1 cut(s)	2469
BspHI	TCATGA	1 cut(s)	2388
BspLI	GGNNCC	2 cut(s)	76, 1409
BspMAI	CTGCAG	1 cut(s)	1423
BspT104I	TTCGAA	1 cut(s)	2710
BspTNI	GGTCTC	1 cut(s)	2933
BsrDI	GCAATG	2 cut(s)	2628, 3099
BsrGI	TGTACA	4 cut(s)	896, 1686, 2169, 2904
BsrI	ACTGG	3 cut(s)	245, 284, 411
BssECI	CCNNGG	2 cut(s)	1059, 1411
BssMI	GATC	7 cut(s)	859, 1329, 2059, 2181, 2308, 2593, 2698
BssNAI	GTATAC	4 cut(s)	582, 1323, 1592, 2523
BssT1I	CCWWGG	2 cut(s)	1059, 1411
Bst1107I	GTATAC	4 cut(s)	582, 1323, 1592, 2523
Bst2UI	CCWGG	3 cut(s)	297, 379, 1397
Bst4CI	ACNGT	5 cut(s)	935, 1220, 1681, 1796, 2448
Bst6I	CTCTTC	3 cut(s)	1959, 2961, 2997
BstAUI	TGTACA	4 cut(s)	896, 1686, 2169, 2904
BstBAI	YACGTR	1 cut(s)	1791
BstBI	TTCGAA	1 cut(s)	2710
BstC8I	GCNNGC	4 cut(s)	660, 1244, 2158, 2658
BstEII	GGTNACC	1 cut(s)	1000
BstENI	CCTNNNNNAGG	1 cut(s)	2783
BstF5I	GGATG	9 cut(s)	522, 1342, 1405, 1842, 2026, 2040, 2370, 2471, 3058
BstFNI	CGCG	1 cut(s)	2469
BstHHI	GCGC	1 cut(s)	2471
BstKTI	GATC	7 cut(s)	862, 1332, 2062, 2184, 2311, 2596, 2701
BstMAI	GTCTC	3 cut(s)	530, 1700, 2933
BstMBI	GATC	7 cut(s)	859, 1329, 2059, 2181, 2308, 2593, 2698
BstMWI	GCNNNNNNNGC	6 cut(s)	608, 1523, 2468, 2639, 2713, 2780
BstNI	CCWGG	3 cut(s)	297, 379, 1397
BstNSI	RCATGY	5 cut(s)	910, 1713, 2160, 2217, 2707
BstPI	GGTNACC	1 cut(s)	1000
BstSCI	CCNGG	3 cut(s)	295, 377, 1395
BstSFI	CTRYAG	4 cut(s)	189, 531, 1419, 2538
BstSLI	GKGCMC	1 cut(s)	1904
BstSNI	TACGTA	1 cut(s)	1791
BstUI	CGCG	1 cut(s)	2469
BstV1I	GCAGC	9 cut(s)	854, 1405, 1447, 1458, 1825, 1933, 2827, 2878, 2953
BstV2I	GAAGAC	1 cut(s)	2474
BstXI	CCANNNNNNTGG	1 cut(s)	1403
BstZ17I	GTATAC	4 cut(s)	582, 1323, 1592, 2523
Bsu15I	ATCGAT	1 cut(s)	1328
Bsu36I	CCTNAGG	3 cut(s)	148, 1519, 1941
BsuI	GTATCC	3 cut(s)	439, 2018, 2123
BsuRI	GGCC	7 cut(s)	263, 571, 1055, 1920, 2265, 2501, 2865
BsuTUI	ATCGAT	1 cut(s)	1328
BtgZI	GCGATG	1 cut(s)	2699
BtsCI	GGATG	9 cut(s)	522, 1342, 1405, 1842, 2026, 2040, 2370, 2471, 3058
BtsI	GCAGTG	1 cut(s)	3068
BtsIMutI	CAGTG	3 cut(s)	206, 252, 3068
Cac8I	GCNNGC	4 cut(s)	660, 1244, 2158, 2658
CciI	TCATGA	1 cut(s)	2388
CfoI	GCGC	1 cut(s)	2471
Cfr13I	GGNCC	2 cut(s)	616, 1393
ClaI	ATCGAT	1 cut(s)	1328
DpnI	GATC	7 cut(s)	861, 1331, 2061, 2183, 2310, 2595, 2700
DpnII	GATC	7 cut(s)	859, 1329, 2059, 2181, 2308, 2593, 2698
DraI	TTTAAA	1 cut(s)	1230
DrdI	GACNNNNNNGTC	1 cut(s)	1714
DseDI	GACNNNNNNGTC	1 cut(s)	1714
EaeI	YGGCCR	3 cut(s)	1053, 2263, 2499
Eam1104I	CTCTTC	3 cut(s)	1959, 2961, 2997
EarI	CTCTTC	3 cut(s)	1959, 2961, 2997
EciI	GGCGGA	1 cut(s)	2270
Ecl136II	GAGCTC	1 cut(s)	128
Eco105I	TACGTA	1 cut(s)	1791
Eco130I	CCWWGG	2 cut(s)	1059, 1411
Eco147I	AGGCCT	1 cut(s)	2865
Eco24I	GRGCYC	2 cut(s)	130, 1134
Eco31I	GGTCTC	1 cut(s)	2933
Eco32I	GATATC	1 cut(s)	3039
Eco47I	GGWCC	2 cut(s)	616, 1393
Eco53kI	GAGCTC	1 cut(s)	128
Eco57I	CTGAAG	3 cut(s)	100, 459, 1616
Eco81I	CCTNAGG	3 cut(s)	148, 1519, 1941
Eco91I	GGTNACC	1 cut(s)	1000
EcoICRI	GAGCTC	1 cut(s)	128
EcoNI	CCTNNNNNAGG	1 cut(s)	2783
EcoO65I	GGTNACC	1 cut(s)	1000
EcoRII	CCWGG	3 cut(s)	295, 377, 1395
EcoRV	GATATC	1 cut(s)	3039
EcoT14I	CCWWGG	2 cut(s)	1059, 1411
EcoT22I	ATGCAT	1 cut(s)	2757
EcoT38I	GRGCYC	2 cut(s)	130, 1134
ErhI	CCWWGG	2 cut(s)	1059, 1411
FalI	AAGNNNNNCTT	6 cut(s)	714, 746, 1258, 1290, 2763, 2795
FaqI	GGGAC	2 cut(s)	1206, 2077
FauNDI	CATATG	2 cut(s)	864, 1440
FbaI	TGATCA	3 cut(s)	859, 2181, 2698
FblI	GTMKAC	4 cut(s)	581, 1322, 1591, 2522
Fnu4HI	GCNGC	9 cut(s)	843, 1419, 1436, 1447, 1839, 1947, 2841, 2892, 2967
FokI	GGATG	9 cut(s)	529, 1349, 1412, 1829, 2033, 2047, 2377, 2458, 3065
FriOI	GRGCYC	2 cut(s)	130, 1134
Fsp4HI	GCNGC	9 cut(s)	843, 1419, 1436, 1447, 1839, 1947, 2841, 2892, 2967
FspBI	CTAG	7 cut(s)	354, 360, 599, 741, 1569, 1910, 2378
GlaI	GCGC	1 cut(s)	2470
GluI	GCNGC	9 cut(s)	843, 1419, 1436, 1447, 1839, 1947, 2841, 2892, 2967
GsaI	CCCAGC	2 cut(s)	1407, 1812
HaeIII	GGCC	7 cut(s)	263, 571, 1055, 1920, 2265, 2501, 2865
HhaI	GCGC	1 cut(s)	2471
Hin6I	GCGC	1 cut(s)	2469
HinP1I	GCGC	1 cut(s)	2469
HindIII	AAGCTT	2 cut(s)	728, 1148
HinfI	GANTC	8 cut(s)	203, 371, 416, 595, 1167, 1367, 2243, 2392
HphI	GGTGA	6 cut(s)	1086, 1523, 1556, 1875, 2891, 3011
Hpy166II	GTNNAC	9 cut(s)	502, 582, 1323, 1592, 1687, 1902, 2170, 2523, 2952
Hpy188III	TCNNGA	6 cut(s)	1748, 1865, 1927, 2389, 2696, 3042
Hpy8I	GTNNAC	9 cut(s)	502, 582, 1323, 1592, 1687, 1902, 2170, 2523, 2952
Hpy99I	CGWCG	1 cut(s)	577
HpyCH4III	ACNGT	5 cut(s)	935, 1220, 1681, 1796, 2448
HpyCH4IV	ACGT	2 cut(s)	1790, 2948
HpyF10VI	GCNNNNNNNGC	6 cut(s)	608, 1523, 2468, 2639, 2713, 2780
HpySE526I	ACGT	2 cut(s)	1790, 2948
HspAI	GCGC	1 cut(s)	2469
Ksp22I	TGATCA	3 cut(s)	859, 2181, 2698
Kzo9I	GATC	7 cut(s)	859, 1329, 2059, 2181, 2308, 2593, 2698
LmnI	GCTCC	3 cut(s)	1523, 2429, 2576
Lsp1109I	GCAGC	9 cut(s)	854, 1405, 1447, 1458, 1825, 1933, 2827, 2878, 2953
LweI	GCATC	6 cut(s)	638, 1390, 1504, 2480, 2676, 2742
MaeI	CTAG	7 cut(s)	354, 360, 599, 741, 1569, 1910, 2378
MaeII	ACGT	2 cut(s)	1790, 2948
MalI	GATC	7 cut(s)	861, 1331, 2061, 2183, 2310, 2595, 2700
MboI	GATC	7 cut(s)	859, 1329, 2059, 2181, 2308, 2593, 2698
MfeI	CAATTG	2 cut(s)	811, 3080
MhlI	GDGCHC	3 cut(s)	130, 1134, 1904
MlsI	TGGCCA	3 cut(s)	1055, 2265, 2501
MluNI	TGGCCA	3 cut(s)	1055, 2265, 2501
MlyI	GAGTC	5 cut(s)	212, 365, 410, 1161, 2252
MmeI	TCCRAC	1 cut(s)	2750
Mox20I	TGGCCA	3 cut(s)	1055, 2265, 2501
Mph1103I	ATGCAT	1 cut(s)	2757
MroXI	GAANNNNTTC	1 cut(s)	272
MscI	TGGCCA	3 cut(s)	1055, 2265, 2501
MslI	CAYNNNNRTG	2 cut(s)	634, 2820
Msp20I	TGGCCA	3 cut(s)	1055, 2265, 2501
MspR9I	CCNGG	3 cut(s)	297, 379, 1397
MunI	CAATTG	2 cut(s)	811, 3080
Mva1269I	GAATGC	3 cut(s)	1290, 1659, 2718
MvaI	CCWGG	3 cut(s)	297, 379, 1397
MvnI	CGCG	1 cut(s)	2469
MwoI	GCNNNNNNNGC	6 cut(s)	608, 1523, 2468, 2639, 2713, 2780
NdeI	CATATG	2 cut(s)	864, 1440
NdeII	GATC	7 cut(s)	859, 1329, 2059, 2181, 2308, 2593, 2698
NlaIV	GGNNCC	2 cut(s)	76, 1409
NmuCI	GTSAC	5 cut(s)	204, 1881, 2216, 2614, 2624
NsiI	ATGCAT	1 cut(s)	2757
NspI	RCATGY	5 cut(s)	910, 1713, 2160, 2217, 2707
NspV	TTCGAA	1 cut(s)	2710
PaeI	GCATGC	1 cut(s)	2160
PagI	TCATGA	1 cut(s)	2388
PceI	AGGCCT	1 cut(s)	2865
PciI	ACATGT	2 cut(s)	1709, 2213
PctI	GAATGC	3 cut(s)	1290, 1659, 2718
PdmI	GAANNNNTTC	1 cut(s)	272
PfeI	GAWTC	3 cut(s)	595, 1367, 2392
PflMI	CCANNNNNTGG	1 cut(s)	251
PfoI	TCCNGGA	1 cut(s)	1395
PkrI	GCNGC	9 cut(s)	844, 1420, 1437, 1448, 1840, 1948, 2842, 2893, 2968
PleI	GAGTC	5 cut(s)	211, 365, 410, 1161, 2251
PpsI	GAGTC	5 cut(s)	211, 365, 410, 1161, 2251
Ppu21I	YACGTR	1 cut(s)	1791
PscI	ACATGT	2 cut(s)	1709, 2213
PshBI	ATTAAT	1 cut(s)	1020
PsiI	TTATAA	2 cut(s)	798, 1113
Psp124BI	GAGCTC	1 cut(s)	130
Psp6I	CCWGG	3 cut(s)	295, 377, 1395
PspEI	GGTNACC	1 cut(s)	1000
PspFI	CCCAGC	2 cut(s)	1403, 1808
PspGI	CCWGG	3 cut(s)	295, 377, 1395
PspN4I	GGNNCC	2 cut(s)	76, 1409
PspPI	GGNCC	2 cut(s)	616, 1393
PstI	CTGCAG	1 cut(s)	1423
RseI	CAYNNNNRTG	2 cut(s)	634, 2820
SacI	GAGCTC	1 cut(s)	130
SatI	GCNGC	9 cut(s)	843, 1419, 1436, 1447, 1839, 1947, 2841, 2892, 2967
Sau3AI	GATC	7 cut(s)	859, 1329, 2059, 2181, 2308, 2593, 2698
Sau96I	GGNCC	2 cut(s)	616, 1393
ScaI	AGTACT	2 cut(s)	688, 1273
SchI	GAGTC	5 cut(s)	212, 365, 410, 1161, 2252
ScrFI	CCNGG	3 cut(s)	297, 379, 1397
SduI	GDGCHC	3 cut(s)	130, 1134, 1904
SfaNI	GCATC	6 cut(s)	638, 1390, 1504, 2480, 2676, 2742
SfcI	CTRYAG	4 cut(s)	189, 531, 1419, 2538
SfuI	TTCGAA	1 cut(s)	2710
SinI	GGWCC	2 cut(s)	616, 1393
SmiMI	CAYNNNNRTG	2 cut(s)	634, 2820
SmlI	CTYRAG	2 cut(s)	505, 2029
SmoI	CTYRAG	2 cut(s)	505, 2029
SnaBI	TACGTA	1 cut(s)	1791
SphI	GCATGC	1 cut(s)	2160
SseBI	AGGCCT	1 cut(s)	2865
SsiI	CCGC	4 cut(s)	1614, 2255, 2409, 2467
SspMI	CTAG	7 cut(s)	354, 360, 599, 741, 1569, 1910, 2378
SstI	GAGCTC	1 cut(s)	130
StuI	AGGCCT	1 cut(s)	2865
StyD4I	CCNGG	3 cut(s)	295, 377, 1395
StyI	CCWWGG	2 cut(s)	1059, 1411
TaaI	ACNGT	5 cut(s)	935, 1220, 1681, 1796, 2448
TaiI	ACGT	2 cut(s)	1793, 2951
TaqI	TCGA	5 cut(s)	419, 1328, 2083, 2710, 2832
TaqII	GACCGA	1 cut(s)	210
TatI	WGTACW	7 cut(s)	686, 896, 1271, 1686, 1986, 2169, 2904
TfiI	GAWTC	3 cut(s)	595, 1367, 2392
TscAI	CASTG	3 cut(s)	213, 252, 3075
TseFI	GTSAC	5 cut(s)	204, 1881, 2216, 2614, 2624
TseI	GCWGC	9 cut(s)	842, 1418, 1435, 1446, 1838, 1946, 2840, 2891, 2966
Tsp45I	GTSAC	5 cut(s)	204, 1881, 2216, 2614, 2624
TspRI	CASTG	3 cut(s)	213, 252, 3075
Van91I	CCANNNNNTGG	1 cut(s)	251
VneI	GTGCAC	1 cut(s)	1900
VpaK11BI	GGWCC	2 cut(s)	616, 1393
VspI	ATTAAT	1 cut(s)	1020
XagI	CCTNNNNNAGG	1 cut(s)	2783
XapI	RAATTY	7 cut(s)	33, 269, 1637, 1723, 1875, 2454, 3046
XceI	RCATGY	5 cut(s)	910, 1713, 2160, 2217, 2707
XcmI	CCANNNNNNNNNTGG	1 cut(s)	385
XmiI	GTMKAC	4 cut(s)	581, 1322, 1591, 2522
XmnI	GAANNNNTTC	1 cut(s)	272
XspI	CTAG	7 cut(s)	354, 360, 599, 741, 1569, 1910, 2378
ZrmI	AGTACT	2 cut(s)	688, 1273
Zsp2I	ATGCAT	1 cut(s)	2757

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.