RchiOBHm_Chr5g0079501

Disease resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0016298
Physical Location & Seq
Reverse (-)
85195363 .. 85195941
579 bp
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UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 579 bp
ATGTTGAATGGCGGTTTTGAAGCATTTGAAGCAACAAGACAAGCCATGGTTGAGCTCATGAAGACATTAGAAAATGATGAGTTCGCTGCCATTGGGGTCTATGGAATGGGAGGAGTTGGGAAGACAACTCTGGTCAAATACCTTAGTGCTATAGCCCTAAAAAAGGGCCTTTTTGATCATGTGATTATGGCTGTTGTATCTCAAACTCCAAATACAAGAAACATTCAAGGCACACTTGCAGATGGTGCTGGGCTTGAAATTGAGGAGGAGTCAGAAATTGGACGAGCCGGTAGATTGCAGAAGGAGATACTTAGAAGAAGTAAGATCCTTATAATCTTGGTCGACATTTGGAATGGACTGGACCTGTCAAGCATTGGAATTCCCAGATATAATGAGCTTCAAATATGCAATTCCAAAGTGCTATTCACAACAAGGAGATTGTATGTCTGTCATTCTATGGAGAGCCAAGCAAACATTCATCTCAGTATCTTATCAGAAGAAGATGCTTGGAACTTGTTTTTGAAGAAGACAAGAAAGTCATTTCAAAAGTCTACAGATTTCTATGAAGTAGCCAGATAG

Protein Analysis

192

Amino Acids

21.52

Weight (kDa)

6.84

Isoelectric Point (pI)

48.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NB-ARC PF00931 15 - 177 7.7e-29 NB-ARC domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 332
AasI GACNNNNNNGTC 1 cut(s) 535
AccI GTMKAC 2 cut(s) 342, 551
AciI CCGC 1 cut(s) 12
AclWI GGATC 1 cut(s) 319
AcsI RAATTY 1 cut(s) 378
AfiI CCNNNNNNNGG 1 cut(s) 163
AgsI TTSAA 8 cut(s) 7, 20, 29, 227, 257, 401, 523, 545
AjuI GAANNNNNNNTTGG 4 cut(s) 407, 439, 459, 491
AluBI AGCT 2 cut(s) 55, 397
AluI AGCT 2 cut(s) 55, 397
Alw21I GWGCWC 1 cut(s) 57
AlwI GGATC 1 cut(s) 319
AoxI GGCC 1 cut(s) 166
ApeKI GCWGC 1 cut(s) 86
ApoI RAATTY 1 cut(s) 378
AspS9I GGNCC 2 cut(s) 166, 361
AvaII GGWCC 1 cut(s) 361
BanII GRGCYC 1 cut(s) 57
BbsI GAAGAC 3 cut(s) 68, 128, 533
Bbv12I GWGCWC 1 cut(s) 57
BbvI GCAGC 1 cut(s) 73
BccI CCATC 1 cut(s) 236
BclI TGATCA 1 cut(s) 175
BfmI CTRYAG 2 cut(s) 150, 552
BisI GCNGC 1 cut(s) 87
BlsI GCNGC 1 cut(s) 88
Bme18I GGWCC 1 cut(s) 361
BmgT120I GGNCC 2 cut(s) 166, 361
BmsI GCATC 1 cut(s) 493
BpiI GAAGAC 3 cut(s) 68, 128, 533
BsaJI CCNNGG 1 cut(s) 45
Bsc4I CCNNNNNNNGG 1 cut(s) 163
Bse118I RCCGGY 1 cut(s) 287
Bse1I ACTGG 1 cut(s) 363
BseDI CCNNGG 1 cut(s) 45
BseLI CCNNNNNNNGG 1 cut(s) 163
BseMII CTCAG 1 cut(s) 496
BseNI ACTGG 1 cut(s) 363
BseRI GAGGAG 3 cut(s) 126, 278, 281
BseXI GCAGC 1 cut(s) 73
BseYI CCCAGC 1 cut(s) 248
BshFI GGCC 1 cut(s) 168
BsiHKAI GWGCWC 1 cut(s) 57
BsiSI CCGG 1 cut(s) 288
BslI CCNNNNNNNGG 1 cut(s) 163
BsnI GGCC 1 cut(s) 168
Bsp1286I GDGCHC 1 cut(s) 57
Bsp143I GATC 2 cut(s) 175, 324
Bsp19I CCATGG 1 cut(s) 45
BspACI CCGC 1 cut(s) 12
BspANI GGCC 1 cut(s) 168
BspCNI CTCAG 1 cut(s) 495
BspHI TCATGA 1 cut(s) 57
BspPI GGATC 1 cut(s) 319
BsrFI RCCGGY 1 cut(s) 287
BsrI ACTGG 1 cut(s) 363
BssAI RCCGGY 1 cut(s) 287
BssECI CCNNGG 1 cut(s) 45
BssMI GATC 2 cut(s) 175, 324
BssT1I CCWWGG 1 cut(s) 45
BstAPI GCANNNNNTGC 1 cut(s) 245
BstDEI CTNAG 3 cut(s) 143, 311, 482
BstDSI CCRYGG 1 cut(s) 45
BstENI CCTNNNNNAGG 1 cut(s) 161
BstKTI GATC 2 cut(s) 178, 327
BstMBI GATC 2 cut(s) 175, 324
BstMWI GCNNNNNNNGC 2 cut(s) 29, 245
BstSFI CTRYAG 2 cut(s) 150, 552
BstV1I GCAGC 1 cut(s) 73
BstV2I GAAGAC 3 cut(s) 68, 128, 533
BstX2I RGATCY 1 cut(s) 324
BstYI RGATCY 1 cut(s) 324
BsuRI GGCC 1 cut(s) 168
BtgI CCRYGG 1 cut(s) 45
CciI TCATGA 1 cut(s) 57
Cfr10I RCCGGY 1 cut(s) 287
Cfr13I GGNCC 2 cut(s) 166, 361
CviAII CATG 3 cut(s) 46, 58, 179
DdeI CTNAG 3 cut(s) 143, 311, 482
DpnI GATC 2 cut(s) 177, 326
DpnII GATC 2 cut(s) 175, 324
DrdI GACNNNNNNGTC 1 cut(s) 535
DseDI GACNNNNNNGTC 1 cut(s) 535
Ecl136II GAGCTC 1 cut(s) 55
Eco130I CCWWGG 1 cut(s) 45
Eco24I GRGCYC 1 cut(s) 57
Eco47I GGWCC 1 cut(s) 361
Eco53kI GAGCTC 1 cut(s) 55
EcoICRI GAGCTC 1 cut(s) 55
EcoNI CCTNNNNNAGG 1 cut(s) 161
EcoO109I RGGNCCY 1 cut(s) 166
EcoRI GAATTC 1 cut(s) 378
EcoT14I CCWWGG 1 cut(s) 45
EcoT38I GRGCYC 1 cut(s) 57
ErhI CCWWGG 1 cut(s) 45
FaeI CATG 3 cut(s) 49, 61, 182
FalI AAGNNNNNCTT 2 cut(s) 219, 251
FatI CATG 3 cut(s) 45, 57, 178
FbaI TGATCA 1 cut(s) 175
FblI GTMKAC 2 cut(s) 342, 551
Fnu4HI GCNGC 1 cut(s) 87
FriOI GRGCYC 1 cut(s) 57
Fsp4HI GCNGC 1 cut(s) 87
GluI GCNGC 1 cut(s) 87
GsaI CCCAGC 1 cut(s) 252
HaeIII GGCC 1 cut(s) 168
HapII CCGG 1 cut(s) 288
Hin1II CATG 3 cut(s) 49, 61, 182
HincII GTYRAC 1 cut(s) 343
HindII GTYRAC 1 cut(s) 343
HinfI GANTC 1 cut(s) 269
HpaII CCGG 1 cut(s) 288
Hpy166II GTNNAC 2 cut(s) 343, 552
Hpy188I TCNGA 2 cut(s) 274, 496
Hpy188III TCNNGA 1 cut(s) 58
Hpy8I GTNNAC 2 cut(s) 343, 552
HpyAV CCTTC 1 cut(s) 295
HpyCH4V TGCA 3 cut(s) 239, 298, 408
HpyF10VI GCNNNNNNNGC 2 cut(s) 29, 245
HpyF3I CTNAG 3 cut(s) 143, 311, 482
Hsp92II CATG 3 cut(s) 49, 61, 182
Ksp22I TGATCA 1 cut(s) 175
Kzo9I GATC 2 cut(s) 175, 324
LpnPI CCDG 6 cut(s) 116, 234, 301, 344, 377, 397
Lsp1109I GCAGC 1 cut(s) 73
LweI GCATC 1 cut(s) 493
MalI GATC 2 cut(s) 177, 326
MboI GATC 2 cut(s) 175, 324
MboII GAAGA 7 cut(s) 73, 133, 327, 509, 512, 535, 538
MflI RGATCY 1 cut(s) 324
MhlI GDGCHC 1 cut(s) 57
MluCI AATT 4 cut(s) 258, 276, 378, 409
MlyI GAGTC 1 cut(s) 278
MnlI CCTC 3 cut(s) 104, 256, 259
MspI CCGG 1 cut(s) 288
MwoI GCNNNNNNNGC 2 cut(s) 29, 245
NcoI CCATGG 1 cut(s) 45
NdeII GATC 2 cut(s) 175, 324
NlaIII CATG 3 cut(s) 49, 61, 182
PagI TCATGA 1 cut(s) 57
PkrI GCNGC 1 cut(s) 88
PleI GAGTC 1 cut(s) 277
PpsI GAGTC 1 cut(s) 277
PsiI TTATAA 1 cut(s) 332
Psp124BI GAGCTC 1 cut(s) 57
PspFI CCCAGC 1 cut(s) 248
PspPI GGNCC 2 cut(s) 166, 361
PsuI RGATCY 1 cut(s) 324
SacI GAGCTC 1 cut(s) 57
SalI GTCGAC 1 cut(s) 341
SatI GCNGC 1 cut(s) 87
Sau3AI GATC 2 cut(s) 175, 324
Sau96I GGNCC 2 cut(s) 166, 361
SchI GAGTC 1 cut(s) 278
SduI GDGCHC 1 cut(s) 57
SetI ASST 4 cut(s) 57, 144, 366, 399
SfaNI GCATC 1 cut(s) 493
SfcI CTRYAG 2 cut(s) 150, 552
SinI GGWCC 1 cut(s) 361
Sse9I AATT 4 cut(s) 258, 276, 378, 409
SsiI CCGC 1 cut(s) 12
SstI GAGCTC 1 cut(s) 57
StyI CCWWGG 1 cut(s) 45
TaqI TCGA 1 cut(s) 342
TasI AATT 4 cut(s) 258, 276, 378, 409
TseI GCWGC 1 cut(s) 86
TspDTI ATGAA 3 cut(s) 74, 467, 579
VpaK11BI GGWCC 1 cut(s) 361
XagI CCTNNNNNAGG 1 cut(s) 161
XapI RAATTY 1 cut(s) 378
XmiI GTMKAC 2 cut(s) 342, 551
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.