Rw7G022040

Disease resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0016298
Physical Location & Seq
Forward (+)
25731607 .. 25732371
765 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G022040.1

Sequence Viewer

Length: 765 bp
ATGGATGAGGTTATGAAGGCGCTTAAAGATGATGAGGTCACTGCCGTTGGTGTCTATGGCATGGGAGGCGTCGGCAAGACAACCATGGTAAACCATGTTGCTGCACAATCCTGCAAAAGTGGGACTTTTCATCATGTGATTATGGCTGTTGTATCCCAAAGCCCCAACTTTGGAAGAATTCAAGACACATTGGCAGATTTATTGGGCGTTAAATTGGAGGGCGTGACAGAAATTGGAAGAGCTGCTAGACTGAATAGGGAGATAATGAGAAGAGAAAAGATCCTTATAATCTTGGATGATGTTTGGAACAGAATAGAATTGTCATCCATAGGAATTCCTAGCTACGAGGAGCTTCAAAAGTGCAATTCCAAAGTCATACTCACCACACGGAGAAGGAATGTCTGTCATGCAATGAAGTGCCAAGAAAAGGTCACCCTCAATATCCTCTCAGAAGAAGATTCTTGGACCTTGTTTGTGAGAAATGCAAGGTCTTTTGAATCGATCACTTTTGAGGGTGTAGCGAGGAAAGTAGCTGGAGAATGCCTACCCATTGCCTTGATAGCGGTTGCAAGGGCACTCGGAGATAAAGATCAGGTGGAATGGCAAAAAGCAGCTCGAAGATTAGAGAAGTCGCAATCTGCAACCGTTGAGGATGAGCAAGATGCCTTCAAATGTATAAAATTTAGCTATGATTACTTGAAAGATGAGGATTCCAAATCATGCTTCTTGCTCTGTTGCCTATACCCAGAAGATGAGGACATCTGA

Protein Analysis

254

Amino Acids

28.53

Weight (kDa)

5.55

Isoelectric Point (pI)

55.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NB-ARC PF00931 1 - 161 1.3e-32 NB-ARC domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 287
AciI CCGC 1 cut(s) 563
AclWI GGATC 1 cut(s) 274
AcsI RAATTY 3 cut(s) 177, 333, 680
AcyI GRCGYC 1 cut(s) 69
AfiI CCNNNNNNNGG 2 cut(s) 170, 427
AgsI TTSAA 5 cut(s) 182, 356, 497, 670, 700
AjuI GAANNNNNNNTTGG 2 cut(s) 707, 739
AluBI AGCT 6 cut(s) 242, 342, 352, 533, 614, 687
AluI AGCT 6 cut(s) 242, 342, 352, 533, 614, 687
AlwI GGATC 1 cut(s) 274
ApeKI GCWGC 3 cut(s) 101, 242, 611
ApoI RAATTY 3 cut(s) 177, 333, 680
AspLEI GCGC 1 cut(s) 22
AspS9I GGNCC 1 cut(s) 465
AsuHPI GGTGA 2 cut(s) 373, 424
AvaII GGWCC 1 cut(s) 465
BaeGI GKGCMC 1 cut(s) 577
BbvI GCAGC 3 cut(s) 88, 229, 623
BceAI ACGGC 1 cut(s) 29
BciVI GTATCC 1 cut(s) 163
BfaI CTAG 2 cut(s) 246, 339
BfoI RGCGCY 1 cut(s) 23
BfuI GTATCC 1 cut(s) 163
BisI GCNGC 3 cut(s) 102, 243, 612
BlsI GCNGC 3 cut(s) 103, 244, 613
Bme18I GGWCC 1 cut(s) 465
BmgT120I GGNCC 1 cut(s) 465
BmsI GCATC 1 cut(s) 652
BpmI CTGGAG 1 cut(s) 555
Bsa29I ATCGAT 1 cut(s) 500
BsaBI GATNNNNATC 1 cut(s) 588
BsaHI GRCGYC 1 cut(s) 69
BsaJI CCNNGG 1 cut(s) 84
Bsc4I CCNNNNNNNGG 2 cut(s) 170, 427
Bse3DI GCAATG 2 cut(s) 417, 549
Bse8I GATNNNNATC 1 cut(s) 588
BseCI ATCGAT 1 cut(s) 500
BseDI CCNNGG 1 cut(s) 84
BseGI GGATG 4 cut(s) 10, 301, 323, 658
BseJI GATNNNNATC 1 cut(s) 588
BseLI CCNNNNNNNGG 2 cut(s) 170, 427
BseMI GCAATG 2 cut(s) 417, 549
BseMII CTCAG 1 cut(s) 462
BseRI GAGGAG 1 cut(s) 362
BseSI GKGCMC 1 cut(s) 577
BseXI GCAGC 3 cut(s) 88, 229, 623
BsgI GTGCAG 1 cut(s) 87
BshVI ATCGAT 1 cut(s) 500
BslFI GGGAC 1 cut(s) 136
BslI CCNNNNNNNGG 2 cut(s) 170, 427
BsmFI GGGAC 1 cut(s) 136
BsmI GAATGC 1 cut(s) 545
Bsp1286I GDGCHC 1 cut(s) 577
Bsp143I GATC 3 cut(s) 279, 501, 589
Bsp19I CCATGG 1 cut(s) 84
BspACI CCGC 1 cut(s) 563
BspCNI CTCAG 1 cut(s) 461
BspDI ATCGAT 1 cut(s) 500
BspPI GGATC 1 cut(s) 274
BspQI GCTCTTC 1 cut(s) 232
BsrDI GCAATG 2 cut(s) 417, 549
BssECI CCNNGG 1 cut(s) 84
BssMI GATC 3 cut(s) 279, 501, 589
BssNI GRCGYC 1 cut(s) 69
BssT1I CCWWGG 1 cut(s) 84
Bst4CI ACNGT 1 cut(s) 646
Bst6I CTCTTC 2 cut(s) 232, 265
BstACI GRCGYC 1 cut(s) 69
BstDEI CTNAG 1 cut(s) 448
BstDSI CCRYGG 1 cut(s) 84
BstEII GGTNACC 1 cut(s) 430
BstF5I GGATG 4 cut(s) 10, 301, 323, 658
BstH2I RGCGCY 1 cut(s) 23
BstHHI GCGC 1 cut(s) 22
BstKTI GATC 3 cut(s) 282, 504, 592
BstMBI GATC 3 cut(s) 279, 501, 589
BstMWI GCNNNNNNNGC 2 cut(s) 66, 560
BstPI GGTNACC 1 cut(s) 430
BstSLI GKGCMC 1 cut(s) 577
BstV1I GCAGC 3 cut(s) 88, 229, 623
BstX2I RGATCY 1 cut(s) 279
BstYI RGATCY 1 cut(s) 279
Bsu15I ATCGAT 1 cut(s) 500
BsuI GTATCC 1 cut(s) 163
BsuTUI ATCGAT 1 cut(s) 500
BtgI CCRYGG 1 cut(s) 84
BtsCI GGATG 4 cut(s) 10, 301, 323, 658
BtsI GCAGTG 1 cut(s) 39
BtsIMutI CAGTG 1 cut(s) 39
CfoI GCGC 1 cut(s) 22
Cfr13I GGNCC 1 cut(s) 465
ClaI ATCGAT 1 cut(s) 500
CseI GACGC 1 cut(s) 58
CviAII CATG 6 cut(s) 61, 85, 95, 134, 407, 720
CviJI RGCY 8 cut(s) 146, 162, 242, 342, 352, 533, 614, 687
CviKI_1 RGCY 8 cut(s) 146, 162, 242, 342, 352, 533, 614, 687
DdeI CTNAG 1 cut(s) 448
DpnI GATC 3 cut(s) 281, 503, 591
DpnII GATC 3 cut(s) 279, 501, 589
Eam1104I CTCTTC 2 cut(s) 232, 265
EarI CTCTTC 2 cut(s) 232, 265
Eco130I CCWWGG 1 cut(s) 84
Eco47I GGWCC 1 cut(s) 465
Eco91I GGTNACC 1 cut(s) 430
EcoO65I GGTNACC 1 cut(s) 430
EcoRI GAATTC 2 cut(s) 177, 333
EcoT14I CCWWGG 1 cut(s) 84
ErhI CCWWGG 1 cut(s) 84
FaeI CATG 6 cut(s) 64, 88, 98, 137, 410, 723
FalI AAGNNNNNCTT 2 cut(s) 109, 141
FaqI GGGAC 1 cut(s) 136
FatI CATG 6 cut(s) 60, 84, 94, 133, 406, 719
Fnu4HI GCNGC 3 cut(s) 102, 243, 612
FokI GGATG 4 cut(s) 17, 308, 310, 665
Fsp4HI GCNGC 3 cut(s) 102, 243, 612
FspBI CTAG 2 cut(s) 246, 339
GlaI GCGC 1 cut(s) 21
GluI GCNGC 3 cut(s) 102, 243, 612
GsuI CTGGAG 1 cut(s) 555
HaeII RGCGCY 1 cut(s) 23
HgaI GACGC 1 cut(s) 58
HhaI GCGC 1 cut(s) 22
Hin1I GRCGYC 1 cut(s) 69
Hin1II CATG 6 cut(s) 64, 88, 98, 137, 410, 723
Hin6I GCGC 1 cut(s) 20
HinP1I GCGC 1 cut(s) 20
HinfI GANTC 3 cut(s) 458, 497, 710
HphI GGTGA 2 cut(s) 373, 424
Hpy166II GTNNAC 1 cut(s) 91
Hpy188I TCNGA 3 cut(s) 451, 581, 764
Hpy188III TCNNGA 1 cut(s) 182
Hpy8I GTNNAC 1 cut(s) 91
Hpy99I CGWCG 1 cut(s) 74
HpyAV CCTTC 3 cut(s) 10, 387, 676
HpyCH4III ACNGT 1 cut(s) 646
HpyCH4V TGCA 7 cut(s) 104, 114, 363, 410, 485, 569, 641
HpyF10VI GCNNNNNNNGC 2 cut(s) 66, 560
HpyF3I CTNAG 1 cut(s) 448
Hsp92I GRCGYC 1 cut(s) 69
Hsp92II CATG 6 cut(s) 64, 88, 98, 137, 410, 723
HspAI GCGC 1 cut(s) 20
Kzo9I GATC 3 cut(s) 279, 501, 589
LguI GCTCTTC 1 cut(s) 232
LmnI GCTCC 1 cut(s) 349
LpnPI CCDG 4 cut(s) 124, 519, 578, 759
Lsp1109I GCAGC 3 cut(s) 88, 229, 623
LweI GCATC 1 cut(s) 652
MaeI CTAG 2 cut(s) 246, 339
MaeIII GTNAC 3 cut(s) 37, 223, 430
MalI GATC 3 cut(s) 281, 503, 591
MboI GATC 3 cut(s) 279, 501, 589
MboII GAAGA 7 cut(s) 186, 249, 282, 464, 467, 630, 761
MflI RGATCY 1 cut(s) 279
MhlI GDGCHC 1 cut(s) 577
MluCI AATT 7 cut(s) 177, 212, 231, 317, 333, 364, 680
MseI TTAA 2 cut(s) 24, 210
Mva1269I GAATGC 1 cut(s) 545
MwoI GCNNNNNNNGC 2 cut(s) 66, 560
NcoI CCATGG 1 cut(s) 84
NdeII GATC 3 cut(s) 279, 501, 589
NlaIII CATG 6 cut(s) 64, 88, 98, 137, 410, 723
NmuCI GTSAC 3 cut(s) 37, 223, 430
PciSI GCTCTTC 1 cut(s) 232
PctI GAATGC 1 cut(s) 545
PfeI GAWTC 3 cut(s) 458, 497, 710
PkrI GCNGC 3 cut(s) 103, 244, 613
PsiI TTATAA 1 cut(s) 287
PspEI GGTNACC 1 cut(s) 430
PspPI GGNCC 1 cut(s) 465
PsuI RGATCY 1 cut(s) 279
SapI GCTCTTC 1 cut(s) 232
SaqAI TTAA 2 cut(s) 24, 210
SatI GCNGC 3 cut(s) 102, 243, 612
Sau3AI GATC 3 cut(s) 279, 501, 589
Sau96I GGNCC 1 cut(s) 465
SduI GDGCHC 1 cut(s) 577
SfaNI GCATC 1 cut(s) 652
SinI GGWCC 1 cut(s) 465
Sse9I AATT 7 cut(s) 177, 212, 231, 317, 333, 364, 680
SsiI CCGC 1 cut(s) 563
SspMI CTAG 2 cut(s) 246, 339
StyI CCWWGG 1 cut(s) 84
TaaI ACNGT 1 cut(s) 646
TaqI TCGA 2 cut(s) 500, 616
TasI AATT 7 cut(s) 177, 212, 231, 317, 333, 364, 680
TfiI GAWTC 3 cut(s) 458, 497, 710
Tru1I TTAA 2 cut(s) 24, 210
Tru9I TTAA 2 cut(s) 24, 210
TscAI CASTG 1 cut(s) 46
TseFI GTSAC 3 cut(s) 37, 223, 430
TseI GCWGC 3 cut(s) 101, 242, 611
Tsp45I GTSAC 3 cut(s) 37, 223, 430
TspDTI ATGAA 3 cut(s) 29, 119, 428
TspGWI ACGGA 1 cut(s) 403
TspRI CASTG 1 cut(s) 46
VpaK11BI GGWCC 1 cut(s) 465
XapI RAATTY 3 cut(s) 177, 333, 680
XspI CTAG 2 cut(s) 246, 339
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.