Rw7G035180

Disease resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0016298
Physical Location & Seq
Reverse (-)
52431668 .. 52432297
630 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G035180.1

Sequence Viewer

Length: 630 bp
ATGGATGAGGTTATGAAGGCGCTAAAAGATGACAAGGTCACTGCTGTGGGGGTCTACGGCATGGGAGGTGTTGGAAAGACAAGCATGGTGAAACATGTCGGTGCACAAGGCTGCAAAAATGGTAACTTTAACCATAAGATTATGGCTGCCATATCTCAAAGCCCTGACTTGAGAAAAATTCAAGGCACATTGGCAGATCTGTTGGGCGTTAAATTGGAGGAGGAGACAGAAATTGGAAGAGCCGCTACGTTGCATAAAGAGATGATGAGAAGAGAAAAACTCCTTATAATCTTGGACGACGTTTGGGAGAGAATAGAGTTGTCAAAGATAGGGATTCCCAGCTACAAGGAGCTTCAAATGTGCAAGTCCAAAGTCCTACTCACCACAAGGATAAGGAATGTCTGTCATGTCATGAGATGCCAGAGAAAGATCACCCTCAGTATCCTATCAAAAGAGGATTCTTGGGCCTTGTTTGTGAGAAATGTAGGAATGCCTTTTGAATCCACCAAATTTAAGGGTGTAGCTAAGAAGGTAGCTGGAGAATGTAGAGGTTTACCGATTGCATTGGTAGCAGTTGCAAGGGCACTTGGAGATAAAGACCTGGCGGAATGGGAAAAAGCAGCTCAATGA

Protein Analysis

209

Amino Acids

23.17

Weight (kDa)

9.43

Isoelectric Point (pI)

28.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NB-ARC PF00931 1 - 163 2e-30 NB-ARC domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 287
AccI GTMKAC 1 cut(s) 54
AciI CCGC 2 cut(s) 243, 605
AcsI RAATTY 2 cut(s) 177, 509
AflIII ACRYGT 1 cut(s) 94
AgsI TTSAA 3 cut(s) 182, 356, 500
AjnI CCWGG 1 cut(s) 600
AleI CACNNNNGTG 1 cut(s) 44
AluBI AGCT 5 cut(s) 342, 352, 524, 536, 623
AluI AGCT 5 cut(s) 342, 352, 524, 536, 623
Alw21I GWGCWC 1 cut(s) 106
Alw26I GTCTC 1 cut(s) 218
Alw44I GTGCAC 1 cut(s) 102
AoxI GGCC 1 cut(s) 465
ApaLI GTGCAC 1 cut(s) 102
ApeKI GCWGC 3 cut(s) 111, 146, 620
ApoI RAATTY 2 cut(s) 177, 509
AspLEI GCGC 1 cut(s) 22
AspS9I GGNCC 1 cut(s) 465
AsuHPI GGTGA 3 cut(s) 100, 373, 424
BaeGI GKGCMC 2 cut(s) 106, 586
BarI GAAGNNNNNNTAC 2 cut(s) 229, 261
Bbv12I GWGCWC 1 cut(s) 106
BbvI GCAGC 2 cut(s) 98, 133
BceAI ACGGC 1 cut(s) 73
BciT130I CCWGG 1 cut(s) 602
BciVI GTATCC 1 cut(s) 452
BcoDI GTCTC 1 cut(s) 218
BfoI RGCGCY 1 cut(s) 23
BfuI GTATCC 1 cut(s) 452
BglII AGATCT 1 cut(s) 196
BisI GCNGC 4 cut(s) 112, 147, 243, 621
BlsI GCNGC 4 cut(s) 113, 148, 244, 622
Bme1390I CCNGG 1 cut(s) 602
BmgT120I GGNCC 1 cut(s) 465
BmrFI CCNGG 1 cut(s) 602
BmsI GCATC 1 cut(s) 407
BplI GAGNNNNNCTC 2 cut(s) 264, 296
BpmI CTGGAG 1 cut(s) 558
BpuEI CTTGAG 1 cut(s) 190
BseBI CCWGG 1 cut(s) 602
BseGI GGATG 1 cut(s) 10
BseMII CTCAG 1 cut(s) 451
BseRI GAGGAG 2 cut(s) 233, 236
BseSI GKGCMC 2 cut(s) 106, 586
BseXI GCAGC 2 cut(s) 98, 133
BseYI CCCAGC 1 cut(s) 338
BshFI GGCC 1 cut(s) 467
BsiHKAI GWGCWC 1 cut(s) 106
BsmAI GTCTC 1 cut(s) 218
BsmI GAATGC 1 cut(s) 495
BsnI GGCC 1 cut(s) 467
Bsp1286I GDGCHC 2 cut(s) 106, 586
Bsp143I GATC 2 cut(s) 196, 429
BspACI CCGC 2 cut(s) 243, 605
BspANI GGCC 1 cut(s) 467
BspCNI CTCAG 1 cut(s) 450
BspHI TCATGA 1 cut(s) 411
BspQI GCTCTTC 1 cut(s) 232
BssMI GATC 2 cut(s) 196, 429
Bst2UI CCWGG 1 cut(s) 602
Bst6I CTCTTC 2 cut(s) 232, 265
BstDEI CTNAG 2 cut(s) 437, 525
BstF5I GGATG 1 cut(s) 10
BstH2I RGCGCY 1 cut(s) 23
BstHHI GCGC 1 cut(s) 22
BstKTI GATC 2 cut(s) 199, 432
BstMAI GTCTC 1 cut(s) 218
BstMBI GATC 2 cut(s) 196, 429
BstMWI GCNNNNNNNGC 1 cut(s) 569
BstNI CCWGG 1 cut(s) 602
BstNSI RCATGY 1 cut(s) 98
BstSCI CCNGG 1 cut(s) 600
BstSLI GKGCMC 2 cut(s) 106, 586
BstV1I GCAGC 2 cut(s) 98, 133
BstX2I RGATCY 1 cut(s) 196
BstYI RGATCY 1 cut(s) 196
BsuI GTATCC 1 cut(s) 452
BsuRI GGCC 1 cut(s) 467
BtsCI GGATG 1 cut(s) 10
BtsI GCAGTG 1 cut(s) 39
BtsIMutI CAGTG 1 cut(s) 39
CciI TCATGA 1 cut(s) 411
CfoI GCGC 1 cut(s) 22
Cfr13I GGNCC 1 cut(s) 465
CviAII CATG 5 cut(s) 61, 85, 95, 407, 412
DdeI CTNAG 2 cut(s) 437, 525
DpnI GATC 2 cut(s) 198, 431
DpnII GATC 2 cut(s) 196, 429
Eam1104I CTCTTC 2 cut(s) 232, 265
EarI CTCTTC 2 cut(s) 232, 265
EciI GGCGGA 1 cut(s) 620
EcoRII CCWGG 1 cut(s) 600
FaeI CATG 5 cut(s) 64, 88, 98, 410, 415
FatI CATG 5 cut(s) 60, 84, 94, 406, 411
FblI GTMKAC 1 cut(s) 54
Fnu4HI GCNGC 4 cut(s) 112, 147, 243, 621
FokI GGATG 1 cut(s) 17
Fsp4HI GCNGC 4 cut(s) 112, 147, 243, 621
GlaI GCGC 1 cut(s) 21
GluI GCNGC 4 cut(s) 112, 147, 243, 621
GsaI CCCAGC 1 cut(s) 342
GsuI CTGGAG 1 cut(s) 558
HaeII RGCGCY 1 cut(s) 23
HaeIII GGCC 1 cut(s) 467
HhaI GCGC 1 cut(s) 22
Hin1II CATG 5 cut(s) 64, 88, 98, 410, 415
Hin6I GCGC 1 cut(s) 20
HinP1I GCGC 1 cut(s) 20
HinfI GANTC 3 cut(s) 334, 458, 500
HphI GGTGA 3 cut(s) 100, 373, 424
Hpy166II GTNNAC 3 cut(s) 55, 104, 554
Hpy188III TCNNGA 1 cut(s) 412
Hpy8I GTNNAC 3 cut(s) 55, 104, 554
Hpy99I CGWCG 1 cut(s) 302
HpyAV CCTTC 2 cut(s) 10, 523
HpyCH4IV ACGT 2 cut(s) 248, 300
HpyCH4V TGCA 6 cut(s) 104, 114, 253, 363, 563, 578
HpyF10VI GCNNNNNNNGC 1 cut(s) 569
HpyF3I CTNAG 2 cut(s) 437, 525
HpySE526I ACGT 2 cut(s) 248, 300
Hsp92II CATG 5 cut(s) 64, 88, 98, 410, 415
HspAI GCGC 1 cut(s) 20
Kzo9I GATC 2 cut(s) 196, 429
LguI GCTCTTC 1 cut(s) 232
LmnI GCTCC 1 cut(s) 349
LpnPI CCDG 6 cut(s) 177, 352, 434, 522, 587, 614
Lsp1109I GCAGC 2 cut(s) 98, 133
LweI GCATC 1 cut(s) 407
MaeII ACGT 2 cut(s) 248, 300
MaeIII GTNAC 2 cut(s) 37, 122
MalI GATC 2 cut(s) 198, 431
MboI GATC 2 cut(s) 196, 429
MboII GAAGA 2 cut(s) 249, 282
MflI RGATCY 1 cut(s) 196
MhlI GDGCHC 2 cut(s) 106, 586
MluCI AATT 4 cut(s) 177, 212, 231, 509
MmeI TCCRAC 1 cut(s) 52
MnlI CCTC 6 cut(s) 59, 211, 214, 446, 448, 542
MseI TTAA 3 cut(s) 129, 210, 513
MslI CAYNNNNRTG 2 cut(s) 44, 99
MspR9I CCNGG 1 cut(s) 602
Mva1269I GAATGC 1 cut(s) 495
MvaI CCWGG 1 cut(s) 602
MwoI GCNNNNNNNGC 1 cut(s) 569
NdeII GATC 2 cut(s) 196, 429
NlaIII CATG 5 cut(s) 64, 88, 98, 410, 415
NmuCI GTSAC 1 cut(s) 37
NspI RCATGY 1 cut(s) 98
OliI CACNNNNGTG 1 cut(s) 44
PagI TCATGA 1 cut(s) 411
PciI ACATGT 1 cut(s) 94
PciSI GCTCTTC 1 cut(s) 232
PctI GAATGC 1 cut(s) 495
PfeI GAWTC 3 cut(s) 334, 458, 500
PflFI GACNNNGTC 1 cut(s) 35
PkrI GCNGC 4 cut(s) 113, 148, 244, 622
PscI ACATGT 1 cut(s) 94
PsiI TTATAA 1 cut(s) 287
Psp6I CCWGG 1 cut(s) 600
PspFI CCCAGC 1 cut(s) 338
PspGI CCWGG 1 cut(s) 600
PspPI GGNCC 1 cut(s) 465
PsuI RGATCY 1 cut(s) 196
PsyI GACNNNGTC 1 cut(s) 35
RseI CAYNNNNRTG 2 cut(s) 44, 99
SapI GCTCTTC 1 cut(s) 232
SaqAI TTAA 3 cut(s) 129, 210, 513
SatI GCNGC 4 cut(s) 112, 147, 243, 621
Sau3AI GATC 2 cut(s) 196, 429
Sau96I GGNCC 1 cut(s) 465
ScrFI CCNGG 1 cut(s) 602
SduI GDGCHC 2 cut(s) 106, 586
SfaNI GCATC 1 cut(s) 407
SmiMI CAYNNNNRTG 2 cut(s) 44, 99
SmlI CTYRAG 1 cut(s) 169
SmoI CTYRAG 1 cut(s) 169
Sse9I AATT 4 cut(s) 177, 212, 231, 509
SsiI CCGC 2 cut(s) 243, 605
StyD4I CCNGG 1 cut(s) 600
TaiI ACGT 2 cut(s) 251, 303
TasI AATT 4 cut(s) 177, 212, 231, 509
TauI GCSGC 1 cut(s) 245
TfiI GAWTC 3 cut(s) 334, 458, 500
Tru1I TTAA 3 cut(s) 129, 210, 513
Tru9I TTAA 3 cut(s) 129, 210, 513
TscAI CASTG 1 cut(s) 46
TseFI GTSAC 1 cut(s) 37
TseI GCWGC 3 cut(s) 111, 146, 620
Tsp45I GTSAC 1 cut(s) 37
TspDTI ATGAA 1 cut(s) 29
TspRI CASTG 1 cut(s) 46
Tth111I GACNNNGTC 1 cut(s) 35
VneI GTGCAC 1 cut(s) 102
XapI RAATTY 2 cut(s) 177, 509
XceI RCATGY 1 cut(s) 98
XmiI GTMKAC 1 cut(s) 54
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.