Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr1

Orthogroup ID

OG0016298

Physical Location & Seq

Genomic Coordinates Reverse (-)

37010237 .. 37019435

Gene Structure 9199 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000002616

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1512 bp

ATGGATGATCAAGATCTTAAAGTAGGGGATGTTATTTCTCCCAGACTTCTAACAGCAATTGAAGAATCAAGGTTTGCAATTGTGGTTCTCTCGCCAAACTATGCCTTTTCTACTTGGTGTTTGGAGGAACTTACACAGATATGTCAATGCATGGAAGACAACAAAATTCTTCCACTTTTTTATAATGTCAGTCCTACTGATATAACAAGGTCACTGCCGTTGGGGTGTACGGCATGGGGCGTTGGAAAGACAACCCTTGTGGAATATGTCGGTCAAGAAGCTCGGAATCAAGGCCTTTTTGATCATGTGATTATGGTTACAGTTAATCAAACCCCTGATTTGAAAAGAATTCAAGGCATATTGGCAGATAGGCTAGGCATCAAATTGATGAACGAGACAGATGAGGGAAGAGCTTCTAGATTGTATTCAGAGACAATGAGAAGAAACAAAACCCTTCTAATCTTGGACAACGTTTGGGGTAGACTAGACCTGCCAAGCATAGGAATTCCCACCTTCGACATTCTTAAGAGTCGCAACTCCAAAGTCCTACTTACCACAAGGAGACATACTGATGGATGTGTTAGGATGCATGATGTCATCCGGGATACAGCCATTCAAATTACACAATCTGAAGATGAGGACGGATTTCTAGTAAAAGTTGGCTGTGGTTTGAAGAATTGGCCATGTCGATTGCGTGAAGGACACTCTGCAATTTCAGTCATGAAGAACGACATTCGAGAGCTACCTGAGGAGCTGAAGCTTGAGATTCTCAGTATGAAGGAATACCCTCTAAAGGAATTGTCAACAGAAATAAGATATTTGACCAATTTAAGGGTGTTGGATGTTAGTGCTGAGGGTTCAGTGGGTGGAGGTATCGTCAGAATTCCATCTGAGGTGATATCAAAATTGCGTAAATTAGAAGAACTGTGCTTGCACAGTGGATTTTGGGACTGTAGGAGTAAAGTTGAGGGACAAGGAGAAACAACTAATGTTAGCTTTGATGAGAGCAAAGGAGGAGTGTCTTCAAAATGGGTAGAGAAAAAGATAGAAAGCTGGTCACGTGAGGCTTATCTCTTCTCCAAAAGGGTCGCCGGCGTTTGTGATATTATCGCTGGGTTGCTGCTGGATCAGCTAAGGCTAGCGGGGCTGGTTACCACTGGTTCTGGGGGAGCAAAGAAGCAGCTGCTGGGCACGAGATTGAAGGTTGATGATCTCACGAGGCTGCTGGTGGTGGCTCCTAGCACTGTTGGCGGATGGTGTTACGCGGTGATAACAGTAATAGCGTGGCTGCTGTGCATTGAGCATAATTGCTGTAAGAGTGCTACGTTGAGAATAACGCGGAATTACCCGCAAATCGGTTGCACTGGAGGAGCTGATGCTGCCAAGAGTCCTCAGATGCGGGTAAAACAGCGATTGTGCAATTCTCAGCCAGCAGCTAATCAAAGGAACACAGCTGAGAACATGGAGGACAAAAGGAACGGTGTAGCAGGGAAGGTGGAATCGGAGTTGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005623 GO:0005886 GO:0006950 GO:0006952 GO:0007154 GO:0007165 GO:0008150 GO:0009987 GO:0016020 GO:0023052 GO:0044464 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0065007 GO:0071944

KEGG Pathways

Metabolic & Signaling

ko04626 map04626

Pfam Domains

Protein Families

LRR_8 NB-ARC TIR

Protein Analysis

504

Amino Acids

55.96

Weight (kDa)

7.82

Isoelectric Point (pI)

43.24

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
TIR_2	PF13676	1 - 63	3.1e-08	TIR domain
TIR	PF01582	2 - 68	2.3e-18	TIR domain
NB-ARC	PF00931	80 - 209	2.3e-14	NB-ARC domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0016298)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_3g43661
rosa_chinensis	RchiOBHm_Chr1g0353701 RchiOBHm_Chr3g0468981 RchiOBHm_Chr5g0076011 RchiOBHm_Chr5g0079501
rosa_laevigata	RLG00000002616 RLG00000002880
rosa_multiflora	Rmu_co7975680.1_g000001 Rmu_co8055250.1_g000001 Rmu_sc0001226.1_g000015 Rmu_sc0009227.1_g000012 Rmu_sc0029401.1_g000001
rosa_rugosa	Rorug05G0363200
rosa_wichuraiana	Rw7G022040 Rw7G035180

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	183
Acc36I	ACCTGC	1 cut(s)	498
AccI	GTMKAC	1 cut(s)	481
AccII	CGCG	2 cut(s)	1265, 1339
AciI	CCGC	6 cut(s)	1142, 1251, 1265, 1339, 1349, 1399
AclI	AACGTT	1 cut(s)	471
AclWI	GGATC	1 cut(s)	1134
AcoI	YGGCCR	1 cut(s)	680
AcsI	RAATTY	4 cut(s)	165, 348, 504, 882
AcuI	CTGAAG	2 cut(s)	651, 776
AcvI	CACGTG	1 cut(s)	1061
AfaI	GTAC	1 cut(s)	229
AfiI	CCNNNNNNNGG	4 cut(s)	500, 793, 831, 1355
AflII	CTTAAG	1 cut(s)	524
AgsI	TTSAA	7 cut(s)	62, 343, 353, 617, 673, 1026, 1201
Alw26I	GTCTC	3 cut(s)	389, 425, 556
AlwI	GGATC	1 cut(s)	1134
AlwNI	CAGNNNCTG	1 cut(s)	1186
AoxI	GGCC	2 cut(s)	292, 680
ApeKI	GCWGC	7 cut(s)	1120, 1180, 1183, 1222, 1288, 1379, 1433
ApoI	RAATTY	4 cut(s)	165, 348, 504, 882
Asp700I	GAANNNNTTC	1 cut(s)	412
AsuC2I	CCSGG	1 cut(s)	602
AsuHPI	GGTGA	2 cut(s)	907, 1279
AsuNHI	GCTAGC	1 cut(s)	1138
AxyI	CCTNAGG	1 cut(s)	747
BaeGI	GKGCMC	1 cut(s)	1193
BalI	TGGCCA	1 cut(s)	682
BauI	CACGAG	2 cut(s)	1192, 1216
BbrPI	CACGTG	1 cut(s)	1061
BbsI	GAAGAC	2 cut(s)	162, 1014
BbvCI	CCTCAGC	1 cut(s)	852
BbvI	GCAGC	7 cut(s)	1107, 1170, 1192, 1209, 1275, 1366, 1445
BccI	CCATC	3 cut(s)	566, 895, 1248
BceAI	ACGGC	2 cut(s)	202, 246
BciVI	GTATCC	1 cut(s)	598
BclI	TGATCA	2 cut(s)	7, 301
BcnI	CCSGG	1 cut(s)	602
BcoDI	GTCTC	3 cut(s)	389, 425, 556
BfaI	CTAG	6 cut(s)	374, 417, 485, 650, 1139, 1239
BfmI	CTRYAG	1 cut(s)	952
BfrI	CTTAAG	1 cut(s)	524
BfuAI	ACCTGC	1 cut(s)	498
BfuI	GTATCC	1 cut(s)	598
BglII	AGATCT	1 cut(s)	13
BisI	GCNGC	7 cut(s)	1121, 1181, 1184, 1223, 1289, 1380, 1434
BlsI	GCNGC	7 cut(s)	1122, 1182, 1185, 1224, 1290, 1381, 1435
Bme1390I	CCNGG	1 cut(s)	602
BmiI	GGNNCC	1 cut(s)	1236
BmrFI	CCNGG	1 cut(s)	602
BmsI	GCATC	4 cut(s)	387, 576, 1366, 1386
BmtI	GCTAGC	1 cut(s)	1142
BpiI	GAAGAC	2 cut(s)	162, 1014
BpmI	CTGGAG	1 cut(s)	1386
Bpu10I	CCTNAGC	2 cut(s)	852, 1133
BpuEI	CTTGAG	1 cut(s)	782
BpuMI	CCSGG	1 cut(s)	602
BsaAI	YACGTR	1 cut(s)	1061
BsaBI	GATNNNNATC	1 cut(s)	12
BsaXI	ACNNNNNCTCC	2 cut(s)	861, 891
Bsc4I	CCNNNNNNNGG	4 cut(s)	500, 793, 831, 1355
Bse118I	RCCGGY	1 cut(s)	1091
Bse1I	ACTGG	2 cut(s)	1162, 1369
Bse21I	CCTNAGG	1 cut(s)	747
Bse8I	GATNNNNATC	1 cut(s)	12
BseGI	GGATG	7 cut(s)	10, 34, 581, 591, 597, 847, 1259
BseJI	GATNNNNATC	1 cut(s)	12
BseLI	CCNNNNNNNGG	4 cut(s)	500, 793, 831, 1355
BseMII	CTCAG	7 cut(s)	738, 784, 843, 882, 1406, 1439, 1446
BseNI	ACTGG	2 cut(s)	1162, 1369
BseRI	GAGGAG	3 cut(s)	764, 1029, 1383
BseSI	GKGCMC	1 cut(s)	1193
BseXI	GCAGC	7 cut(s)	1107, 1170, 1192, 1209, 1275, 1366, 1445
BseYI	CCCAGC	2 cut(s)	1112, 1186
Bsh1236I	CGCG	2 cut(s)	1265, 1339
BshFI	GGCC	2 cut(s)	294, 682
BsiSI	CCGG	2 cut(s)	601, 1092
BslFI	GGGAC	2 cut(s)	962, 984
BslI	CCNNNNNNNGG	4 cut(s)	500, 793, 831, 1355
BsmAI	GTCTC	3 cut(s)	389, 425, 556
BsmFI	GGGAC	2 cut(s)	962, 984
BsnI	GGCC	2 cut(s)	294, 682
Bsp1286I	GDGCHC	1 cut(s)	1193
Bsp143I	GATC	5 cut(s)	7, 13, 301, 1126, 1210
BspACI	CCGC	6 cut(s)	1142, 1251, 1265, 1339, 1349, 1399
BspANI	GGCC	2 cut(s)	294, 682
BspCNI	CTCAG	7 cut(s)	739, 783, 844, 883, 1405, 1438, 1447
BspFNI	CGCG	2 cut(s)	1265, 1339
BspHI	TCATGA	1 cut(s)	720
BspLI	GGNNCC	1 cut(s)	1236
BspMI	ACCTGC	1 cut(s)	498
BspOI	GCTAGC	1 cut(s)	1142
BspPI	GGATC	1 cut(s)	1134
BspQI	GCTCTTC	1 cut(s)	403
BspTI	CTTAAG	1 cut(s)	524
BsrFI	RCCGGY	1 cut(s)	1091
BsrI	ACTGG	2 cut(s)	1162, 1369
BssAI	RCCGGY	1 cut(s)	1091
BssMI	GATC	5 cut(s)	7, 13, 301, 1126, 1210
BssSI	CACGAG	2 cut(s)	1192, 1216
Bst2BI	CACGAG	2 cut(s)	1192, 1216
Bst4CI	ACNGT	7 cut(s)	322, 927, 938, 953, 1246, 1276, 1481
Bst6I	CTCTTC	2 cut(s)	403, 1079
BstAFI	CTTAAG	1 cut(s)	524
BstBAI	YACGTR	1 cut(s)	1061
BstC8I	GCNNGC	4 cut(s)	932, 1093, 1140, 1431
BstDEI	CTNAG	8 cut(s)	747, 770, 852, 891, 1133, 1392, 1425, 1455
BstEII	GGTNACC	1 cut(s)	1150
BstF5I	GGATG	7 cut(s)	10, 34, 581, 591, 597, 847, 1259
BstFNI	CGCG	2 cut(s)	1265, 1339
BstKTI	GATC	5 cut(s)	10, 16, 304, 1129, 1213
BstMAI	GTCTC	3 cut(s)	389, 425, 556
BstMBI	GATC	5 cut(s)	7, 13, 301, 1126, 1210
BstMWI	GCNNNNNNNGC	4 cut(s)	1129, 1144, 1248, 1379
BstPI	GGTNACC	1 cut(s)	1150
BstSCI	CCNGG	1 cut(s)	600
BstSFI	CTRYAG	1 cut(s)	952
BstSLI	GKGCMC	1 cut(s)	1193
BstUI	CGCG	2 cut(s)	1265, 1339
BstV1I	GCAGC	7 cut(s)	1107, 1170, 1192, 1209, 1275, 1366, 1445
BstV2I	GAAGAC	2 cut(s)	162, 1014
BstX2I	RGATCY	1 cut(s)	13
BstYI	RGATCY	1 cut(s)	13
Bsu36I	CCTNAGG	1 cut(s)	747
BsuI	GTATCC	1 cut(s)	598
BsuRI	GGCC	2 cut(s)	294, 682
BtsCI	GGATG	7 cut(s)	10, 34, 581, 591, 597, 847, 1259
BtsI	GCAGTG	1 cut(s)	212
BtsIMutI	CAGTG	6 cut(s)	212, 867, 943, 1155, 1242, 1362
BveI	ACCTGC	1 cut(s)	498
Cac8I	GCNNGC	4 cut(s)	932, 1093, 1140, 1431
CaiI	CAGNNNCTG	1 cut(s)	1186
CciI	TCATGA	1 cut(s)	720
Cfr10I	RCCGGY	1 cut(s)	1091
Csp6I	GTAC	1 cut(s)	228
CspCI	CAANNNNNGTGG	2 cut(s)	240, 275
CviAII	CATG	7 cut(s)	151, 234, 305, 590, 684, 721, 1462
CviQI	GTAC	1 cut(s)	228
DdeI	CTNAG	8 cut(s)	747, 770, 852, 891, 1133, 1392, 1425, 1455
DpnI	GATC	5 cut(s)	9, 15, 303, 1128, 1212
DpnII	GATC	5 cut(s)	7, 13, 301, 1126, 1210
EaeI	YGGCCR	1 cut(s)	680
Eam1104I	CTCTTC	2 cut(s)	403, 1079
EarI	CTCTTC	2 cut(s)	403, 1079
EciI	GGCGGA	1 cut(s)	1266
Eco147I	AGGCCT	1 cut(s)	294
Eco32I	GATATC	1 cut(s)	900
Eco57I	CTGAAG	2 cut(s)	651, 776
Eco72I	CACGTG	1 cut(s)	1061
Eco81I	CCTNAGG	1 cut(s)	747
Eco91I	GGTNACC	1 cut(s)	1150
EcoO65I	GGTNACC	1 cut(s)	1150
EcoRI	GAATTC	3 cut(s)	348, 504, 882
EcoRV	GATATC	1 cut(s)	900
EcoT22I	ATGCAT	2 cut(s)	152, 591
FaeI	CATG	7 cut(s)	154, 237, 308, 593, 687, 724, 1465
FalI	AAGNNNNNCTT	2 cut(s)	534, 566
FaqI	GGGAC	2 cut(s)	962, 984
FatI	CATG	7 cut(s)	150, 233, 304, 589, 683, 720, 1461
FauI	CCCGC	3 cut(s)	1135, 1356, 1392
FbaI	TGATCA	2 cut(s)	7, 301
FblI	GTMKAC	1 cut(s)	481
Fnu4HI	GCNGC	7 cut(s)	1121, 1181, 1184, 1223, 1289, 1380, 1434
FokI	GGATG	7 cut(s)	17, 41, 584, 588, 598, 854, 1266
Fsp4HI	GCNGC	7 cut(s)	1121, 1181, 1184, 1223, 1289, 1380, 1434
FspBI	CTAG	6 cut(s)	374, 417, 485, 650, 1139, 1239
GluI	GCNGC	7 cut(s)	1121, 1181, 1184, 1223, 1289, 1380, 1434
GsaI	CCCAGC	2 cut(s)	1116, 1190
GsuI	CTGGAG	1 cut(s)	1386
HaeIII	GGCC	2 cut(s)	294, 682
HapII	CCGG	2 cut(s)	601, 1092
Hin1II	CATG	7 cut(s)	154, 237, 308, 593, 687, 724, 1465
HincII	GTYRAC	1 cut(s)	804
HindII	GTYRAC	1 cut(s)	804
HindIII	AAGCTT	1 cut(s)	758
HinfI	GANTC	6 cut(s)	65, 286, 529, 766, 1387, 1499
HpaII	CCGG	2 cut(s)	601, 1092
HphI	GGTGA	2 cut(s)	907, 1279
Hpy166II	GTNNAC	3 cut(s)	228, 482, 804
Hpy188I	TCNGA	7 cut(s)	285, 430, 631, 881, 892, 1395, 1504
Hpy188III	TCNNGA	6 cut(s)	11, 275, 417, 721, 737, 1216
Hpy8I	GTNNAC	3 cut(s)	228, 482, 804
HpyAV	CCTTC	6 cut(s)	464, 523, 692, 772, 1195, 1486
HpyCH4III	ACNGT	7 cut(s)	322, 927, 938, 953, 1246, 1276, 1481
HpyCH4IV	ACGT	3 cut(s)	471, 1060, 1325
HpyCH4V	TGCA	8 cut(s)	77, 150, 589, 710, 934, 1296, 1362, 1419
HpyF10VI	GCNNNNNNNGC	4 cut(s)	1129, 1144, 1248, 1379
HpyF3I	CTNAG	8 cut(s)	747, 770, 852, 891, 1133, 1392, 1425, 1455
HpySE526I	ACGT	3 cut(s)	471, 1060, 1325
Hsp92II	CATG	7 cut(s)	154, 237, 308, 593, 687, 724, 1465
KroI	GCCGGC	1 cut(s)	1091
KroNI	GCCGGC	1 cut(s)	1093
Ksp22I	TGATCA	2 cut(s)	7, 301
Kzo9I	GATC	5 cut(s)	7, 13, 301, 1126, 1210
LguI	GCTCTTC	1 cut(s)	403
LmnI	GCTCC	4 cut(s)	751, 1169, 1240, 1370
Lsp1109I	GCAGC	7 cut(s)	1107, 1170, 1192, 1209, 1275, 1366, 1445
LweI	GCATC	4 cut(s)	387, 576, 1366, 1386
MaeI	CTAG	6 cut(s)	374, 417, 485, 650, 1139, 1239
MaeII	ACGT	3 cut(s)	471, 1060, 1325
MaeIII	GTNAC	5 cut(s)	210, 316, 1056, 1150, 1259
MalI	GATC	5 cut(s)	9, 15, 303, 1128, 1212
MboI	GATC	5 cut(s)	7, 13, 301, 1126, 1210
MfeI	CAATTG	2 cut(s)	57, 78
MflI	RGATCY	1 cut(s)	13
MhlI	GDGCHC	1 cut(s)	1193
MlsI	TGGCCA	1 cut(s)	682
MluNI	TGGCCA	1 cut(s)	682
MlyI	GAGTC	2 cut(s)	538, 1396
MmeI	TCCRAC	2 cut(s)	223, 819
Mox20I	TGGCCA	1 cut(s)	682
Mph1103I	ATGCAT	2 cut(s)	152, 591
MreI	CGCCGGCG	1 cut(s)	1091
MroNI	GCCGGC	1 cut(s)	1091
MroXI	GAANNNNTTC	1 cut(s)	412
MscI	TGGCCA	1 cut(s)	682
MseI	TTAA	4 cut(s)	18, 324, 525, 830
MslI	CAYNNNNRTG	2 cut(s)	139, 570
Msp20I	TGGCCA	1 cut(s)	682
MspA1I	CMGCKG	2 cut(s)	1183, 1454
MspCI	CTTAAG	1 cut(s)	524
MspI	CCGG	2 cut(s)	601, 1092
MspR9I	CCNGG	1 cut(s)	602
MunI	CAATTG	2 cut(s)	57, 78
MvnI	CGCG	2 cut(s)	1265, 1339
MwoI	GCNNNNNNNGC	4 cut(s)	1129, 1144, 1248, 1379
NaeI	GCCGGC	1 cut(s)	1093
NciI	CCSGG	1 cut(s)	602
NdeII	GATC	5 cut(s)	7, 13, 301, 1126, 1210
NgoMIV	GCCGGC	1 cut(s)	1091
NheI	GCTAGC	1 cut(s)	1138
NlaIII	CATG	7 cut(s)	154, 237, 308, 593, 687, 724, 1465
NlaIV	GGNNCC	1 cut(s)	1236
NmuCI	GTSAC	2 cut(s)	210, 1056
NsiI	ATGCAT	2 cut(s)	152, 591
PagI	TCATGA	1 cut(s)	720
PceI	AGGCCT	1 cut(s)	294
PciSI	GCTCTTC	1 cut(s)	403
PdiI	GCCGGC	1 cut(s)	1093
PdmI	GAANNNNTTC	1 cut(s)	412
PfeI	GAWTC	4 cut(s)	65, 286, 766, 1499
PfoI	TCCNGGA	1 cut(s)	600
PkrI	GCNGC	7 cut(s)	1122, 1182, 1185, 1224, 1290, 1381, 1435
PleI	GAGTC	2 cut(s)	537, 1395
PmaCI	CACGTG	1 cut(s)	1061
PmlI	CACGTG	1 cut(s)	1061
PpsI	GAGTC	2 cut(s)	537, 1395
Ppu21I	YACGTR	1 cut(s)	1061
PsiI	TTATAA	1 cut(s)	183
Psp1406I	AACGTT	1 cut(s)	471
PspCI	CACGTG	1 cut(s)	1061
PspEI	GGTNACC	1 cut(s)	1150
PspFI	CCCAGC	2 cut(s)	1112, 1186
PspN4I	GGNNCC	1 cut(s)	1236
PstNI	CAGNNNCTG	1 cut(s)	1186
PsuI	RGATCY	1 cut(s)	13
PvuII	CAGCTG	2 cut(s)	1183, 1454
RsaI	GTAC	1 cut(s)	229
RsaNI	GTAC	1 cut(s)	228
RseI	CAYNNNNRTG	2 cut(s)	139, 570
SapI	GCTCTTC	1 cut(s)	403
SaqAI	TTAA	4 cut(s)	18, 324, 525, 830
SatI	GCNGC	7 cut(s)	1121, 1181, 1184, 1223, 1289, 1380, 1434
Sau3AI	GATC	5 cut(s)	7, 13, 301, 1126, 1210
SchI	GAGTC	2 cut(s)	538, 1396
ScrFI	CCNGG	1 cut(s)	602
SduI	GDGCHC	1 cut(s)	1193
SfaNI	GCATC	4 cut(s)	387, 576, 1366, 1386
SfcI	CTRYAG	1 cut(s)	952
SgrAI	CRCCGGYG	1 cut(s)	1091
SmiMI	CAYNNNNRTG	2 cut(s)	139, 570
SmlI	CTYRAG	2 cut(s)	524, 761
SmoI	CTYRAG	2 cut(s)	524, 761
SseBI	AGGCCT	1 cut(s)	294
SsiI	CCGC	6 cut(s)	1142, 1251, 1265, 1339, 1349, 1399
SspMI	CTAG	6 cut(s)	374, 417, 485, 650, 1139, 1239
StuI	AGGCCT	1 cut(s)	294
StyD4I	CCNGG	1 cut(s)	600
TaaI	ACNGT	7 cut(s)	322, 927, 938, 953, 1246, 1276, 1481
TaiI	ACGT	3 cut(s)	474, 1063, 1328
TaqI	TCGA	3 cut(s)	516, 688, 736
TaqII	GACCGA	1 cut(s)	260
TfiI	GAWTC	4 cut(s)	65, 286, 766, 1499
Tru1I	TTAA	4 cut(s)	18, 324, 525, 830
Tru9I	TTAA	4 cut(s)	18, 324, 525, 830
TscAI	CASTG	6 cut(s)	219, 867, 943, 1162, 1249, 1369
TseFI	GTSAC	2 cut(s)	210, 1056
TseI	GCWGC	7 cut(s)	1120, 1180, 1183, 1222, 1288, 1379, 1433
Tsp45I	GTSAC	2 cut(s)	210, 1056
TspDTI	ATGAA	3 cut(s)	404, 737, 791
TspGWI	ACGGA	1 cut(s)	657
TspRI	CASTG	6 cut(s)	219, 867, 943, 1162, 1249, 1369
Vha464I	CTTAAG	1 cut(s)	524
XapI	RAATTY	4 cut(s)	165, 348, 504, 882
XbaI	TCTAGA	1 cut(s)	416
XmiI	GTMKAC	1 cut(s)	481
XmnI	GAANNNNTTC	1 cut(s)	412
XspI	CTAG	6 cut(s)	374, 417, 485, 650, 1139, 1239
Zsp2I	ATGCAT	2 cut(s)	152, 591

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.