RchiOBHm_Chr4g0413501

Rapid ALkalinization Factor (RALF)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0014259
Physical Location & Seq
Forward (+)
37739408 .. 37740151
744 bp
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UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 456 bp
ATGGCTGACCCCTTTATGAGCCATCCAACTACCCAGAATCCCACTCTCCCAGTCCCAAAAACAAAAACACAAAGAAGCCCCAAAACAGAGAACATCAAAATGTCCAAACTCAGAGACAATCCCATCTTAATCTTGTATCTTGGCCTCCTCTTAATCCTTACCCATTTCTCAATCTGCAATGGGGCGTCAGTTCTTGACCTCAGTGCACTCAAAGGAAGTGAAATTGATGTCAAGGTTAGGGACTTCATAACAGAGCCAGAGATGGACTCAGAGATAAGTAGAAGAGTGCTAGCAATGCAGAAGAAGTACATAAGCTATGAGACACTGAGGAGGGACTTGGTTCCTTGTGCAAGGCCAGGGGCTTCATACTACAATTGCCATGCTGTGGCCAACCCTTATAATAGAGGCTGTGAGGTTATCACAGGATGTAGAGGTACACATGACATCAAAACTTGA

Protein Analysis

151

Amino Acids

16.95

Weight (kDa)

9.01

Isoelectric Point (pI)

51.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RALF PF05498 85 - 145 7.4e-27 Rapid ALkalinization Factor (RALF)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014259)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G23805 AT4G13950
fragaria_vesca FvH4_4g13250
malus_domestica MD04G1017300.v1.1 MD13G1281800.v1.1
prunus_persica Prupe.1G165400_v2.0.a1
rosa_chinensis RchiOBHm_Chr4g0413501
rosa_laevigata RLG00000008215
rosa_multiflora Rmu_sc0000883.1_g000006
rosa_roxburghii Rroxscaffold_5G00357400
rosa_rugosa Rorug04G0120700
rosa_samantha Rh4AG180400 Rh4BG179700 Rh4CG191500 Rh4DG175700
rosa_wichuraiana Rw4G015030 Rw4G015080

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 399
AccB7I CCANNNNNTGG 1 cut(s) 385
AcoI YGGCCR 1 cut(s) 387
AcyI GRCGYC 1 cut(s) 185
AfaI GTAC 2 cut(s) 308, 436
AfiI CCNNNNNNNGG 1 cut(s) 385
AjnI CCWGG 1 cut(s) 355
AluBI AGCT 1 cut(s) 315
AluI AGCT 1 cut(s) 315
Alw21I GWGCWC 1 cut(s) 208
Alw26I GTCTC 2 cut(s) 108, 314
Alw44I GTGCAC 1 cut(s) 204
AoxI GGCC 3 cut(s) 142, 353, 387
ApaLI GTGCAC 1 cut(s) 204
AsuNHI GCTAGC 1 cut(s) 289
BaeGI GKGCMC 1 cut(s) 208
BalI TGGCCA 1 cut(s) 389
Bbv12I GWGCWC 1 cut(s) 208
BccI CCATC 3 cut(s) 30, 131, 256
BciT130I CCWGG 1 cut(s) 357
BcoDI GTCTC 2 cut(s) 108, 314
BfaI CTAG 1 cut(s) 290
Bme1390I CCNGG 1 cut(s) 357
BmiI GGNNCC 1 cut(s) 342
BmrFI CCNGG 1 cut(s) 357
BmrI ACTGGG 1 cut(s) 44
BmtI GCTAGC 1 cut(s) 293
BmuI ACTGGG 1 cut(s) 44
BplI GAGNNNNNCTC 2 cut(s) 251, 283
BsaHI GRCGYC 1 cut(s) 185
BsaJI CCNNGG 1 cut(s) 356
Bsc4I CCNNNNNNNGG 1 cut(s) 385
Bse1I ACTGG 1 cut(s) 50
Bse3DI GCAATG 2 cut(s) 184, 300
BseBI CCWGG 1 cut(s) 357
BseDI CCNNGG 1 cut(s) 356
BseGI GGATG 2 cut(s) 22, 431
BseLI CCNNNNNNNGG 1 cut(s) 385
BseMI GCAATG 2 cut(s) 184, 300
BseMII CTCAG 4 cut(s) 124, 214, 282, 317
BseNI ACTGG 1 cut(s) 50
BseRI GAGGAG 2 cut(s) 137, 343
BseSI GKGCMC 1 cut(s) 208
BshFI GGCC 3 cut(s) 144, 355, 389
BsiHKAI GWGCWC 1 cut(s) 208
BslFI GGGAC 3 cut(s) 38, 254, 347
BslI CCNNNNNNNGG 1 cut(s) 385
BsmAI GTCTC 2 cut(s) 108, 314
BsmFI GGGAC 3 cut(s) 38, 254, 347
BsnI GGCC 3 cut(s) 144, 355, 389
Bsp1286I GDGCHC 1 cut(s) 208
BspANI GGCC 3 cut(s) 144, 355, 389
BspCNI CTCAG 4 cut(s) 123, 213, 281, 318
BspLI GGNNCC 1 cut(s) 342
BspOI GCTAGC 1 cut(s) 293
BsrDI GCAATG 2 cut(s) 184, 300
BsrI ACTGG 1 cut(s) 50
BssECI CCNNGG 1 cut(s) 356
BssNI GRCGYC 1 cut(s) 185
Bst2UI CCWGG 1 cut(s) 357
Bst6I CTCTTC 1 cut(s) 277
BstACI GRCGYC 1 cut(s) 185
BstC8I GCNNGC 1 cut(s) 291
BstDEI CTNAG 4 cut(s) 110, 200, 268, 326
BstF5I GGATG 2 cut(s) 22, 431
BstMAI GTCTC 2 cut(s) 108, 314
BstMWI GCNNNNNNNGC 1 cut(s) 295
BstNI CCWGG 1 cut(s) 357
BstSCI CCNGG 1 cut(s) 355
BstSLI GKGCMC 1 cut(s) 208
BsuRI GGCC 3 cut(s) 144, 355, 389
BtsCI GGATG 2 cut(s) 22, 431
BtsIMutI CAGTG 2 cut(s) 208, 323
Cac8I GCNNGC 1 cut(s) 291
CseI GACGC 1 cut(s) 174
Csp6I GTAC 2 cut(s) 307, 435
CviAII CATG 2 cut(s) 380, 440
CviQI GTAC 2 cut(s) 307, 435
DdeI CTNAG 4 cut(s) 110, 200, 268, 326
EaeI YGGCCR 1 cut(s) 387
Eam1104I CTCTTC 1 cut(s) 277
EarI CTCTTC 1 cut(s) 277
EcoRII CCWGG 1 cut(s) 355
FaeI CATG 2 cut(s) 383, 443
FaiI YATR 8 cut(s) 17, 248, 311, 318, 367, 381, 399, 441
FaqI GGGAC 3 cut(s) 38, 254, 347
FatI CATG 2 cut(s) 379, 439
FokI GGATG 2 cut(s) 9, 438
FspBI CTAG 1 cut(s) 290
HaeIII GGCC 3 cut(s) 144, 355, 389
HgaI GACGC 1 cut(s) 174
Hin1I GRCGYC 1 cut(s) 185
Hin1II CATG 2 cut(s) 383, 443
HinfI GANTC 2 cut(s) 37, 266
Hpy166II GTNNAC 2 cut(s) 206, 437
Hpy188I TCNGA 2 cut(s) 113, 271
Hpy188III TCNNGA 1 cut(s) 194
Hpy8I GTNNAC 2 cut(s) 206, 437
HpyCH4V TGCA 4 cut(s) 177, 206, 298, 350
HpyF10VI GCNNNNNNNGC 1 cut(s) 295
HpyF3I CTNAG 4 cut(s) 110, 200, 268, 326
Hsp92I GRCGYC 1 cut(s) 185
Hsp92II CATG 2 cut(s) 383, 443
LpnPI CCDG 6 cut(s) 47, 63, 270, 342, 369, 408
MaeI CTAG 1 cut(s) 290
MboII GAAGA 2 cut(s) 294, 313
MfeI CAATTG 1 cut(s) 373
MhlI GDGCHC 1 cut(s) 208
MlsI TGGCCA 1 cut(s) 389
MluCI AATT 2 cut(s) 222, 373
MluNI TGGCCA 1 cut(s) 389
MlyI GAGTC 1 cut(s) 260
MmeI TCCRAC 1 cut(s) 50
MnlI CCTC 8 cut(s) 155, 158, 209, 321, 324, 398, 406, 425
Mox20I TGGCCA 1 cut(s) 389
MscI TGGCCA 1 cut(s) 389
MseI TTAA 2 cut(s) 128, 152
MslI CAYNNNNRTG 1 cut(s) 98
Msp20I TGGCCA 1 cut(s) 389
MspR9I CCNGG 1 cut(s) 357
MunI CAATTG 1 cut(s) 373
MvaI CCWGG 1 cut(s) 357
MwoI GCNNNNNNNGC 1 cut(s) 295
NheI GCTAGC 1 cut(s) 289
NlaIII CATG 2 cut(s) 383, 443
NlaIV GGNNCC 1 cut(s) 342
PfeI GAWTC 1 cut(s) 37
PflMI CCANNNNNTGG 1 cut(s) 385
PleI GAGTC 1 cut(s) 260
PpsI GAGTC 1 cut(s) 260
PsiI TTATAA 1 cut(s) 399
Psp6I CCWGG 1 cut(s) 355
PspGI CCWGG 1 cut(s) 355
PspN4I GGNNCC 1 cut(s) 342
RsaI GTAC 2 cut(s) 308, 436
RsaNI GTAC 2 cut(s) 307, 435
RseI CAYNNNNRTG 1 cut(s) 98
SaqAI TTAA 2 cut(s) 128, 152
SchI GAGTC 1 cut(s) 260
ScrFI CCNGG 1 cut(s) 357
SduI GDGCHC 1 cut(s) 208
SetI ASST 5 cut(s) 201, 237, 317, 417, 436
SmiMI CAYNNNNRTG 1 cut(s) 98
Sse9I AATT 2 cut(s) 222, 373
SspMI CTAG 1 cut(s) 290
StyD4I CCNGG 1 cut(s) 355
TasI AATT 2 cut(s) 222, 373
TatI WGTACW 1 cut(s) 306
TfiI GAWTC 1 cut(s) 37
Tru1I TTAA 2 cut(s) 128, 152
Tru9I TTAA 2 cut(s) 128, 152
TscAI CASTG 2 cut(s) 208, 330
TspDTI ATGAA 2 cut(s) 235, 354
TspRI CASTG 2 cut(s) 208, 330
Van91I CCANNNNNTGG 1 cut(s) 385
VneI GTGCAC 1 cut(s) 204
XspI CTAG 1 cut(s) 290
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.