RchiOBHm_Chr4g0415381

calcium-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0008345
Physical Location & Seq
Reverse (-)
40049602 .. 40052947
3346 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 711 bp
ATGAAGGCTTTGAGTGATTCTTCTTTGTCAGACAAATTAGGAGGCATGCTTTGTTGTCGTGGTTCGGCAAACAAGTATAGCAGGCTGGATGCAAAGCTTGAAAAGAGAATGGCTGAATTTAAAAGAGGTTCTTCAGGCCAAAGCAATTTTAAGTCAATTGACAGCATAATTATGAGGTTCCCTCAGTTTAGGGAAGAACTGAAGAACATAAGAAATGTGTTTGACCTGTATGATGAGGACTCAAATGGAGCTATAGACCGCGAGGAACTGAAGAAATGCTTACAGAAACTGCAACTCCATCTGACAGCAGAAGAGATTGAGGATCTTTTTCATTCATGTGACATGGATGGGAGTGAAGGGATACAATTCAATGAATTCATTGTTCTTCTATGTCTCATCTATCTATTAAAGGACCCTCAAACGACATCGAAGATAGACTCTCCACAGCTAGAAGCAACTTTTGATACTGTCATTGAAGTATTTTTGTTTCTGGATAAAAATGGTGATGGAAAACTGAACAAGACAGAAGTGGTGAAGGCACTGAATGAAGCTTCTCCGCGGGAGAAATCTCCTGCTCATGTCAGCAGGAATCGATTCAAGGAAATGGATTGGGACAAAAGTGGGAAGGTGAGTTTCAGGCAGTTCCTCTTTGCTTTCATCGATTGGGTTGGAATTGAAACAGATGAAGAAATATCTCTAGAACAAAGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0005509 GO:0043167 GO:0043169 GO:0046872
KEGG Pathways
Metabolic & Signaling
ko04626 map04626
Pfam Domains
Protein Families
EF-hand_1 EF-hand_5 EF-hand_6 EF-hand_7 EF-hand_8

Protein Analysis

236

Amino Acids

27.03

Weight (kDa)

5.05

Isoelectric Point (pI)

50.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_7 PF13499 69 - 127 1.6e-11 EF-hand domain pair
EF-hand_1 PF00036 70 - 97 6.8e-06 EF hand domain
EF-hand_6 PF13405 70 - 97 6.2e-07 EF-hand domain
EF-hand_8 PF13833 81 - 130 6e-09 EF-hand domain pair
EF-hand_7 PF13499 157 - 218 5.1e-07 EF-hand domain pair
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 261, 559
AciI CCGC 3 cut(s) 259, 557, 559
AclWI GGATC 1 cut(s) 330
AcsI RAATTY 2 cut(s) 116, 374
AcuI CTGAAG 3 cut(s) 117, 221, 290
AgsI TTSAA 5 cut(s) 101, 370, 476, 598, 677
AluBI AGCT 4 cut(s) 97, 251, 448, 551
AluI AGCT 4 cut(s) 97, 251, 448, 551
Alw26I GTCTC 1 cut(s) 398
AlwI GGATC 1 cut(s) 330
AlwNI CAGNNNCTG 1 cut(s) 289
AoxI GGCC 1 cut(s) 136
ApoI RAATTY 2 cut(s) 116, 374
Asp700I GAANNNNTTC 1 cut(s) 593
AspS9I GGNCC 1 cut(s) 412
AsuHPI GGTGA 3 cut(s) 515, 544, 640
AvaII GGWCC 1 cut(s) 412
BccI CCATC 3 cut(s) 306, 341, 500
BciVI GTATCC 1 cut(s) 354
BcoDI GTCTC 1 cut(s) 398
BfaI CTAG 2 cut(s) 449, 698
BfmI CTRYAG 1 cut(s) 252
BfuI GTATCC 1 cut(s) 354
Bme18I GGWCC 1 cut(s) 412
BmgT120I GGNCC 1 cut(s) 412
BmiI GGNNCC 2 cut(s) 179, 414
BmsI GCATC 1 cut(s) 79
BplI GAGNNNNNCTC 2 cut(s) 166, 198
Bsa29I ATCGAT 2 cut(s) 592, 660
BsaJI CCNNGG 1 cut(s) 557
BsaXI ACNNNNNCTCC 2 cut(s) 279, 309
BseCI ATCGAT 2 cut(s) 592, 660
BseDI CCNNGG 1 cut(s) 557
BseGI GGATG 2 cut(s) 94, 352
BseMII CTCAG 1 cut(s) 197
Bsh1236I CGCG 2 cut(s) 261, 559
BshFI GGCC 1 cut(s) 138
BshVI ATCGAT 2 cut(s) 592, 660
BslFI GGGAC 1 cut(s) 626
BsmAI GTCTC 1 cut(s) 398
BsmFI GGGAC 1 cut(s) 626
BsnI GGCC 1 cut(s) 138
Bsp143I GATC 1 cut(s) 322
BspACI CCGC 3 cut(s) 259, 557, 559
BspANI GGCC 1 cut(s) 138
BspCNI CTCAG 1 cut(s) 196
BspDI ATCGAT 2 cut(s) 592, 660
BspFNI CGCG 2 cut(s) 261, 559
BspLI GGNNCC 2 cut(s) 179, 414
BspPI GGATC 1 cut(s) 330
BssECI CCNNGG 1 cut(s) 557
BssMI GATC 1 cut(s) 322
Bst4CI ACNGT 1 cut(s) 469
Bst6I CTCTTC 1 cut(s) 306
BstC8I GCNNGC 2 cut(s) 47, 83
BstDEI CTNAG 1 cut(s) 183
BstDSI CCRYGG 1 cut(s) 557
BstF5I GGATG 2 cut(s) 94, 352
BstFNI CGCG 2 cut(s) 261, 559
BstKTI GATC 1 cut(s) 325
BstMAI GTCTC 1 cut(s) 398
BstMBI GATC 1 cut(s) 322
BstNSI RCATGY 1 cut(s) 49
BstSFI CTRYAG 1 cut(s) 252
BstUI CGCG 2 cut(s) 261, 559
BstX2I RGATCY 1 cut(s) 322
BstYI RGATCY 1 cut(s) 322
Bsu15I ATCGAT 2 cut(s) 592, 660
BsuI GTATCC 1 cut(s) 354
BsuRI GGCC 1 cut(s) 138
BsuTUI ATCGAT 2 cut(s) 592, 660
BtgI CCRYGG 1 cut(s) 557
BtsCI GGATG 2 cut(s) 94, 352
BtsIMutI CAGTG 1 cut(s) 539
Cac8I GCNNGC 2 cut(s) 47, 83
CaiI CAGNNNCTG 1 cut(s) 289
Cfr13I GGNCC 1 cut(s) 412
Cfr42I CCGCGG 1 cut(s) 560
ClaI ATCGAT 2 cut(s) 592, 660
CviAII CATG 4 cut(s) 46, 336, 343, 578
CviJI RGCY 8 cut(s) 8, 85, 97, 113, 138, 251, 448, 551
CviKI_1 RGCY 8 cut(s) 8, 85, 97, 113, 138, 251, 448, 551
DdeI CTNAG 1 cut(s) 183
DpnI GATC 1 cut(s) 324
DpnII GATC 1 cut(s) 322
DraI TTTAAA 1 cut(s) 121
Eam1104I CTCTTC 1 cut(s) 306
EarI CTCTTC 1 cut(s) 306
Eco47I GGWCC 1 cut(s) 412
Eco57I CTGAAG 3 cut(s) 117, 221, 290
EcoO109I RGGNCCY 1 cut(s) 412
EcoRI GAATTC 1 cut(s) 374
FaeI CATG 4 cut(s) 49, 339, 346, 581
FalI AAGNNNNNCTT 4 cut(s) 115, 147, 263, 295
FaqI GGGAC 1 cut(s) 626
FatI CATG 4 cut(s) 45, 335, 342, 577
FauI CCCGC 1 cut(s) 552
FokI GGATG 2 cut(s) 101, 359
FspBI CTAG 2 cut(s) 449, 698
HaeIII GGCC 1 cut(s) 138
Hin1II CATG 4 cut(s) 49, 339, 346, 581
HindIII AAGCTT 2 cut(s) 95, 549
HinfI GANTC 5 cut(s) 17, 239, 437, 589, 594
HphI GGTGA 3 cut(s) 515, 544, 640
Hpy188I TCNGA 2 cut(s) 31, 303
Hpy188III TCNNGA 2 cut(s) 491, 698
HpyAV CCTTC 3 cut(s) 350, 529, 619
HpyCH4III ACNGT 1 cut(s) 469
HpyCH4V TGCA 2 cut(s) 92, 292
HpyF3I CTNAG 1 cut(s) 183
Hsp92II CATG 4 cut(s) 49, 339, 346, 581
KspI CCGCGG 1 cut(s) 560
Kzo9I GATC 1 cut(s) 322
LmnI GCTCC 1 cut(s) 248
LpnPI CCDG 8 cut(s) 67, 71, 120, 239, 476, 571, 585, 622
LweI GCATC 1 cut(s) 79
MaeI CTAG 2 cut(s) 449, 698
MaeIII GTNAC 1 cut(s) 338
MalI GATC 1 cut(s) 324
MboI GATC 1 cut(s) 322
MboII GAAGA 9 cut(s) 12, 123, 206, 214, 283, 323, 377, 442, 698
MfeI CAATTG 1 cut(s) 156
MflI RGATCY 1 cut(s) 322
MluCI AATT 8 cut(s) 35, 116, 145, 156, 168, 365, 374, 672
MlyI GAGTC 2 cut(s) 233, 431
MmeI TCCRAC 1 cut(s) 649
MnlI CCTC 9 cut(s) 35, 119, 168, 192, 229, 256, 313, 426, 656
MroXI GAANNNNTTC 1 cut(s) 593
MseI TTAA 4 cut(s) 120, 150, 407, 709
MslI CAYNNNNRTG 2 cut(s) 170, 336
MspA1I CMGCKG 1 cut(s) 559
MunI CAATTG 1 cut(s) 156
MvnI CGCG 2 cut(s) 261, 559
NdeII GATC 1 cut(s) 322
NlaIII CATG 4 cut(s) 49, 339, 346, 581
NlaIV GGNNCC 2 cut(s) 179, 414
NmuCI GTSAC 1 cut(s) 338
NspI RCATGY 1 cut(s) 49
PaeI GCATGC 1 cut(s) 49
PdmI GAANNNNTTC 1 cut(s) 593
PfeI GAWTC 3 cut(s) 17, 589, 594
PleI GAGTC 2 cut(s) 233, 431
PpsI GAGTC 2 cut(s) 233, 431
PpuMI RGGWCCY 1 cut(s) 412
Psp5II RGGWCCY 1 cut(s) 412
PspN4I GGNNCC 2 cut(s) 179, 414
PspPI GGNCC 1 cut(s) 412
PspPPI RGGWCCY 1 cut(s) 412
PstNI CAGNNNCTG 1 cut(s) 289
PsuI RGATCY 1 cut(s) 322
RseI CAYNNNNRTG 2 cut(s) 170, 336
SacII CCGCGG 1 cut(s) 560
SaqAI TTAA 4 cut(s) 120, 150, 407, 709
Sau3AI GATC 1 cut(s) 322
Sau96I GGNCC 1 cut(s) 412
SchI GAGTC 2 cut(s) 233, 431
SetI ASST 8 cut(s) 99, 130, 179, 228, 253, 450, 553, 630
SfaNI GCATC 1 cut(s) 79
SfcI CTRYAG 1 cut(s) 252
Sfr303I CCGCGG 1 cut(s) 560
SgrBI CCGCGG 1 cut(s) 560
SinI GGWCC 1 cut(s) 412
SmiMI CAYNNNNRTG 2 cut(s) 170, 336
SphI GCATGC 1 cut(s) 49
Sse9I AATT 8 cut(s) 35, 116, 145, 156, 168, 365, 374, 672
SsiI CCGC 3 cut(s) 259, 557, 559
SspMI CTAG 2 cut(s) 449, 698
TaaI ACNGT 1 cut(s) 469
TaqI TCGA 3 cut(s) 428, 592, 660
TasI AATT 8 cut(s) 35, 116, 145, 156, 168, 365, 374, 672
TfiI GAWTC 3 cut(s) 17, 589, 594
Tru1I TTAA 4 cut(s) 120, 150, 407, 709
Tru9I TTAA 4 cut(s) 120, 150, 407, 709
TscAI CASTG 1 cut(s) 546
TseFI GTSAC 1 cut(s) 338
Tsp45I GTSAC 1 cut(s) 338
TspDTI ATGAA 8 cut(s) 17, 320, 324, 367, 387, 561, 646, 699
TspRI CASTG 1 cut(s) 546
VpaK11BI GGWCC 1 cut(s) 412
XapI RAATTY 2 cut(s) 116, 374
XbaI TCTAGA 1 cut(s) 697
XceI RCATGY 1 cut(s) 49
XmnI GAANNNNTTC 1 cut(s) 593
XspI CTAG 2 cut(s) 449, 698
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.