RchiOBHm_Chr4g0421271

Pentatricopeptide repeat-containing protein At3g18110

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0015414
Physical Location & Seq
Reverse (-)
46879653 .. 46887638
7986 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 4530 bp
ATGACATTCACAGGAGTGCTGTCCCTGGCTACCTCACCCTTATTCCATTCAGAGTCCCCACTCTCATCTTCTTCTCCTTCTTCTTCAAATAAGGTATGCAAAAGCACTTGTCTTTCTTGCTCTCTAACTCAAGTACCTTCTACTTCTAGTTCTGGTAGTTCTGAGCAGCCTAATGATAGTAACACTAGTCAAATTAACAACAACAAGAGATTTAGCTATAGCAGAGCTTCACCCTCAGTCAGATGGCCTCATTTGAAGCTCTCAGAAACTTACCCTTCACCTCCTCAGACCCAATTCACCCCTCTTGTGGAGGATGCCGGGTTTTCGACCCAACTCTCTGATTCTGAGTCTGAGAGCAAAGAGGAAGATAATGGTGATGTGGGTTTTGCAGCATCATCTTTGGATAAGAATGATGAAACCCAGTTAGTGTTGGGGAGGCCCAGCAAGACCAGGGCAAAGAAAATGACCAAATTGGCTCTTAAGAGAGCCAAGGATTGGAGGGAGAGAGTCAAGTTCTTTACTGATAGGATTTTGGGGTTGAAATCTGATGAGTTTGTGGCTGATGTGCTGGATGATAGGAAAGTACAGATGACCCCCACTGATTTTTGCTTTGTGGTGAAATGGGTGGGGCAGAAGAGCTGGCAGCGCGCGTTGGAGGTGTACGAGTGGCTGAATTTGTGCCACTGGTATGCCCCGAATGCACGAATGCTTGCAACTATCTTGGCTGTTCTTGGAAAGGCTAACCAGGAAGCTTTGGCTGTGGAGATATACACCAGGGCTGAGCCTGAAATTGGTAATACAGTCCAGGTGTACAATGCAATGATGGGTGTGTATGCACGAAGTGGTCGGTTTAAGAGGGTGCAAGAACTGCTCAACTTAATGCGTGAGAGGGGGTGTGAGCCAGACCTTGTGAGTCTCAACACTTTGATAAATGCTCGTCTCAGGTCAGGTGCCATGGTACCAAATTTGGCTATTGAACTTTTGAATGAGGTGAGGAGGTCGGGTCTTAGGCCAGATATAATCACCTACAATACTCTTATAAGCGGCTGTGCTCGGGAATCCAATTTGGAGGAGGCCGTGAAGGTATACGCTGACATGGAAGGACATAACTGTCAACCTGATCTTTGGACTTATAATGCCATGATTTCGGTTTATGGCAGATGTGGGCAGTCGAGCAAAGCTGAGCAGCTCTTTAAGGAATTGGAGTCAAAAGGGTTTTTCCCTGATGCAGTGACATATAATTCTTTGTTGTATGCTTTTGCAAGAGAACTTAATATAGAAAAAGTCCGGGATATTGGTGAAGAGATGGTGAAAATGGGGTTTGCTAAAGATGAGATGACATACAATACTATTATCCACATGTATGGAAAGCAGGGCCAGCATGATCAAGCACAGCAGATTTACCGGGACATGAAAATGTTGGGTCGAATTCCTGATGCTGTTACGTACACTGTTTTGATAGATTCCCTTGGCAAAGAAAATAAGATTACAGAGGCTGCAAATGTGATGTCAGAAATGTTGGACAGTGGAGTAAAACCCACTCTTCGGACTTATAGTGCTTTAATGTGTGGATATGCGAAGGCTGGCAAACAAGTGGAGGCTCAAGAAACATTTGATTGCATGATTAAGTCTGGGATTAGACCAGATCATCTAGCATACTCAGTTATGCTTGATATCTTTCTCAGGTCCAACGAGACAAAGAAGGCAATGACATTGTACCAGGAAATGCTGCATGATGGTTTCATGCCAGATAATGCCCTTTACGAGGTAATGTTGCGGGTGCTTGGAACTGAAAACAAATTGGAAACTATTGAAAAAGTGATTAGAGACATGGAAAAGGTCGGTGGTATGAATGCACAGGTCATCTCTTCAATTCTTGTTAAGGGAGAATGCTATGATCATGCTGCTAAAATGTTAAGATTAGCCATCACCAGTGGTTATGAACTGGACCGGGAAAGTCTTTTTTCTATTCTCAGTTCATATAGTTCTTGTGGTAGACACTCAGAAGCATGTGAGTTGCTTGCATTTTTGAAAGAACATGCTCCCGGTTCCAACCAACTGATAACTGAGGCAATGGTTGTCATACAATGCAAGGCTGGTGAATTTGATGCTGCCTTGGCAGAATATAATAATTCCAAGGGTTTCCATTCTTTTAGCAGAAGTTGTACTATGTATGAAATCTTGATTCAAGGCTGTGAGAAGAATGAACTCTTTGGTGAAGCTTCACAGGTTTATTCTGACATGAGATTCTATGGTATTGAACCATCAGAGCATCTTTACCAAAATATGGTGCTGATATATTGTAAAATGGGTTTCCCCGAGACAGCCCACCATTTGATTGAGCAGGCAGACATGAAAGGCATTCTTTTTGACAGTATCTCCATATATGTTGATGTTATTGAAGTATATGGGAAATTGAAGCTATGGCAGAAAGCTGAAAGTTTGGTGGGAAGTCTTAAACAAAGATGTAAAACAGTGGATAGGAAGGTCTGGAATGCCTTGATACAAGCTTATGCCGCAAGTGGTTGCTATGAGCGGGCTAGAGTGATCTTTAACACAATGACAAGAGATGGTCCTTCCCCAACGGTAGAGTCTGTTAATGGTCTTTTGCAGGCTTTAATTGTTGATGGGAGGTTGGACGAACTTTATGTTCTTATCCAGGAGTTGCAAGATATGGGTTTTAAAATAAGCAAGAGTTCAATTCTGTTGATGCTTGAAGCATTTGCTCGAGCTGGTAACATATTTGAGGTGAAGAAAATATATCATGGAATGAAGGCTGCTGGTTATTTTCCTACAATGGATAGTTTCAGAATTATGATCAAGTTATTGTGCAGGGGGAAACGAGTAAGGGATGTTGAAGCAATGGTTTCTGAGATGGAAGAGGCGGGTTTCAAGCCTGATCTTTCAATATGGAATTCCTTGCTTAAATTATATGCAGGAATTGAGGATTTCAAAAAGACAGTTAATGTATACCAGCAGATTAAAGAAGCTGAACTTCAACCAGATGAAGATACTTACAATACATTAATTATATTGTACTGCAGAGATCGTAGACCAGAAGAAGGTTTGTCGTTGATGCATGAAATGAGAAGGCAGGGCCTGGAACCGAAGCTGAACACTTACAAAAGCTTAATTTCAGCATTTGGTAAGCAGAAGTTGTTGGATCAAGCTGAGGAACTTTTTGAAGAGTTAAGGTCTAATGGATGTAAATTGGATCGGTCATTTTATCATACGATGATGAAACTATTTAGAAATTCTGGAAACCATGCCAAAGCTGAAATGCTGCTGTCTGTGATGAAGGAGGCTGGAATAGAACCCAACTTTGCCACAATGCACTTGCTTATGGTTTCTTATGGCAGCTCTGGACAGCCTCAGGAAGCAGAAAAAGTGCTTGACAATTTGAAAGTAACTGGTTTAGATCTGGATACGTTACCCTATAGTTCAGTTATTGATGCTTATCTAAGGAATGGAGATTATAACACTGGAATTCAAAAGCTCAATGAGATGAAGAAAGGTGGTCTGGAGCCAGATCATAGAATATGGACATGCTTCATAAGGGCTGCAAGTTTGTCCCAGCAGACAAGTGAAGCCTTTGTTCTTCTAAATGCACTACGTGATGCTGGATTTGATCTCCCAATTAGGCTTTTGAAGGAAAAATCTGAGTCACTCATTCCAGATGTAGACCACTGTCTAGAGAAACTAGAACCCTTAGAAGACAATGCAGCCTTCAATTTTGTCAATGCTCTGGAGGACCTCTTGTGGGCATATGAACTCCGGGCTACTGCTTCATGGGTTTTCCAACTGGCAGTAAAGAGAGGCATCTATAACCGTGATGTGTTCAGAGTCGCTGACAAGGACTGGGGTGCTGATTTTAGGAAGTTGTCAGCTGGTTCTGCTCTTGTTGGTCTTACTTTATGGCTTGATCAGATGCAGGCAACTCTCTTTCTCCTCTGTAGTTTCGATGCATCCTTGGAGGGTTATCCAGAGTCTCCCAAGTCAGTCGTTCTGATAACAGGGACATCTGAGTACAACATGGTTTCACTTAACAGTACATTAAAGGCGTGCCTTTGGGAAATTGGTTCACCTTTTCTGCCGTGTAAAACACGAAGTGGTCTACTTGTAGCAAAAGCACACTCCCTCAGAATGTGGTTAAAGGACTCCCCGTTTTGTTTGGACCTTGAGTTGAAAGATGCGCCAGCTCTCCCTGAATCAAACTCAATGCAGCTCATTGATGGATGCTTCTTAAGACGTGGTCTTGTTCCTGCATTTAAAGAAATAAATGAGAAGCTTGGATTAGTGAGACCCAAGAAATTTGCAAGACTGGCTTTGCTATCTGATGAAAAAAGAGAAAGGGTTATTCAAGCTGATATCGAGGGAAGGAAAGAGAAGTTAGAAAAAATGAGAAAGAGGGGAATTGTTGACCCGAGTAGGGTGAGCAGGATTAGGAAGCTTAGAAAGAGGACATGTATAAGACCGTCCATGTTATCAAACACCAAACAAATTGCATCAAAAAGAAGAGCTTTGGATTAG

Protein Analysis

1509

Amino Acids

170.39

Weight (kDa)

6.69

Isoelectric Point (pI)

42.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPR_24 PF23276 237 - 365 4.4e-09 Fungal tetratrico peptide repeats
PPR_2 PF13041 268 - 312 1.3e-09 PPR repeat family
PPR PF01535 270 - 299 8.6e-06 PPR repeat
PPR_2 PF13041 301 - 352 2.9e-10 PPR repeat family
PPR_long PF17177 324 - 463 5.2e-11 Pentacotripeptide-repeat region of PRORP
PPR_3 PF13812 327 - 386 8e-15 Pentatricopeptide repeat domain
PPR_1 PF12854 335 - 367 8.3e-09 PPR repeat
PPR_2 PF13041 338 - 383 1.3e-14 PPR repeat family
PPR PF01535 341 - 371 5.2e-07 PPR repeat
PPR_1 PF12854 370 - 401 1.6e-06 PPR repeat
PPR_2 PF13041 377 - 422 4e-12 PPR repeat family
PPR PF01535 377 - 406 4.8e-08 PPR repeat
PPR_3 PF13812 397 - 457 2.4e-07 Pentatricopeptide repeat domain
PPR_3 PF13812 432 - 492 1.9e-06 Pentatricopeptide repeat domain
PPR_2 PF13041 444 - 491 1.8e-14 PPR repeat family
PPR_long PF17177 462 - 562 3.9e-08 Pentacotripeptide-repeat region of PRORP
PPR_1 PF12854 478 - 506 8.3e-06 PPR repeat
PPR_2 PF13041 478 - 527 1e-12 PPR repeat family
PPR_3 PF13812 502 - 560 1.1e-07 Pentatricopeptide repeat domain
PPR_2 PF13041 517 - 559 2.6e-07 PPR repeat family
TPR_24 PF23276 886 - 994 1.7e-07 Fungal tetratrico peptide repeats
PPR_long PF17177 915 - 1010 2.1e-06 Pentacotripeptide-repeat region of PRORP
PPR_3 PF13812 956 - 1013 9.2e-07 Pentatricopeptide repeat domain
PPR_2 PF13041 966 - 1010 9.6e-07 PPR repeat family
PPR_long PF17177 986 - 1071 5.9e-08 Pentacotripeptide-repeat region of PRORP
PPR_2 PF13041 1001 - 1050 1.1e-10 PPR repeat family
PPR PF01535 1005 - 1034 5e-07 PPR repeat
PPR_3 PF13812 1024 - 1083 7.2e-08 Pentatricopeptide repeat domain
PPR PF01535 1040 - 1069 7.6e-06 PPR repeat
TPR_24 PF23276 1120 - 1215 2.5e-06 Fungal tetratrico peptide repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0015414)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G18110
fragaria_vesca FvH4_4g17300 FvH4_4g17300
malus_domestica MD07G1304500.v1.1
prunus_persica Prupe.6G174500_v2.0.a1
pyrus_communis pycom07g27730
rosa_chinensis RchiOBHm_Chr4g0421271
rosa_laevigata RLG00000007705
rosa_multiflora Rmu_sc0009290.1_g000019
rosa_roxburghii Rroxscaffold_5G00363500
rosa_rugosa Rorug04G0169600
rosa_samantha Rh4AG230100 Rh4BG232900 Rh4CG244500 Rh4DG228700
rosa_wichuraiana Rw4G020360

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 1040, 1134, 3477
AasI GACNNNNNNGTC 1 cut(s) 1110
Acc65I GGTACC 1 cut(s) 958
AccB1I GGYRCC 2 cut(s) 950, 958
AccB7I CCANNNNNTGG 2 cut(s) 495, 2287
AccBSI CCGCTC 1 cut(s) 2536
AccI GTMKAC 6 cut(s) 1086, 1996, 2970, 3052, 3681, 4114
AccII CGCG 2 cut(s) 648, 650
AciI CCGC 5 cut(s) 1044, 1777, 2517, 2536, 2885
AclWI GGATC 2 cut(s) 3171, 3222
AcsI RAATTY 8 cut(s) 673, 964, 1428, 2102, 2914, 3253, 3486, 4310
AdeI CACNNNGTG 3 cut(s) 842, 3614, 4109
AfiI CCNNNNNNNGG 9 cut(s) 307, 495, 791, 1545, 1765, 2287, 3062, 3760, 4429
AflII CTTAAG 2 cut(s) 479, 4243
AflIII ACRYGT 2 cut(s) 1359, 4463
AhlI ACTAGT 1 cut(s) 185
AjiI CACGTC 1 cut(s) 4250
AjnI CCWGG 8 cut(s) 24, 449, 744, 773, 804, 1719, 2658, 3099
AleI CACNNNNGTG 1 cut(s) 14
Alw21I GWGCWC 1 cut(s) 1054
Alw26I GTCTC 7 cut(s) 920, 944, 1688, 1821, 2315, 3993, 4294
AlwI GGATC 2 cut(s) 3171, 3222
AlwNI CAGNNNCTG 2 cut(s) 1496, 3100
Ama87I CYCGRG 4 cut(s) 1053, 2318, 2727, 4423
AoxI GGCC 6 cut(s) 245, 437, 1010, 1074, 1375, 3097
ApoI RAATTY 8 cut(s) 673, 964, 1428, 2102, 2914, 3253, 3486, 4310
ArsI GACNNNNNNTTYG 2 cut(s) 1493, 1525
AseI ATTAAT 1 cut(s) 3026
Asp718I GGTACC 1 cut(s) 958
AspLEI GCGC 3 cut(s) 648, 650, 4195
AspS9I GGNCC 8 cut(s) 438, 1375, 1686, 1948, 2573, 3097, 3751, 4174
AsuC2I CCSGG 6 cut(s) 319, 1289, 1406, 1952, 2046, 3776
AvaI CYCGRG 4 cut(s) 1053, 2318, 2727, 4423
AvaII GGWCC 5 cut(s) 1686, 1948, 2573, 3751, 4174
AxyI CCTNAGG 1 cut(s) 3372
BaeI ACNNNNGTAYC 2 cut(s) 117, 150
BanI GGYRCC 2 cut(s) 950, 958
BbsI GAAGAC 1 cut(s) 3720
Bbv12I GWGCWC 1 cut(s) 1054
BbvCI CCTCAGC 1 cut(s) 3171
BceAI ACGGC 2 cut(s) 1061, 4078
BciT130I CCWGG 8 cut(s) 26, 451, 746, 775, 806, 1721, 2660, 3101
BciVI GTATCC 1 cut(s) 3418
BclI TGATCA 4 cut(s) 1384, 1897, 2817, 3922
BcnI CCSGG 6 cut(s) 319, 1289, 1406, 1952, 2046, 3776
BcoDI GTCTC 7 cut(s) 920, 944, 1688, 1821, 2315, 3993, 4294
BcuI ACTAGT 1 cut(s) 185
BfaI CTAG 6 cut(s) 147, 186, 1652, 2541, 3692, 3701
BfmI CTRYAG 4 cut(s) 217, 3040, 3436, 3952
BfrI CTTAAG 2 cut(s) 479, 4243
BfuI GTATCC 1 cut(s) 3418
BglII AGATCT 1 cut(s) 3418
BlpI GCTNAGC 2 cut(s) 780, 1182
Bme18I GGWCC 5 cut(s) 1686, 1948, 2573, 3751, 4174
BmeT110I CYCGRG 4 cut(s) 1053, 2318, 2727, 4423
BmgBI CACGTC 1 cut(s) 4250
BmgT120I GGNCC 8 cut(s) 438, 1375, 1686, 1948, 2573, 3097, 3751, 4174
BmiI GGNNCC 5 cut(s) 952, 960, 2050, 3105, 3525
BmrI ACTGGG 2 cut(s) 415, 3868
BmuI ACTGGG 2 cut(s) 415, 3868
BoxI GACNNNNGTC 1 cut(s) 3687
BpiI GAAGAC 1 cut(s) 3720
BpmI CTGGAG 2 cut(s) 3542, 3767
Bpu10I CCTNAGC 1 cut(s) 3171
Bpu1102I GCTNAGC 2 cut(s) 780, 1182
BpuEI CTTGAG 3 cut(s) 114, 1587, 4199
BpuMI CCSGG 6 cut(s) 319, 1289, 1406, 1952, 2046, 3776
BsaAI YACGTR 2 cut(s) 1446, 3614
BsaBI GATNNNNATC 1 cut(s) 3456
BsaI GGTCTC 1 cut(s) 4294
BsaJI CCNNGG 9 cut(s) 24, 450, 489, 774, 954, 1468, 2115, 2136, 3969
BsaXI ACNNNNNCTCC 4 cut(s) 988, 1018, 2363, 2393
Bsc4I CCNNNNNNNGG 9 cut(s) 307, 495, 791, 1545, 1765, 2287, 3062, 3760, 4429
Bse1I ACTGG 9 cut(s) 421, 689, 1932, 1950, 3415, 3487, 3807, 3863, 4326
Bse21I CCTNAGG 1 cut(s) 3372
Bse3DI GCAATG 4 cut(s) 825, 1713, 2079, 2868
Bse8I GATNNNNATC 1 cut(s) 3456
BseBI CCWGG 8 cut(s) 26, 451, 746, 775, 806, 1721, 2660, 3101
BseDI CCNNGG 9 cut(s) 24, 450, 489, 774, 954, 1468, 2115, 2136, 3969
BseGI GGATG 6 cut(s) 319, 577, 2857, 3209, 3965, 4241
BseJI GATNNNNATC 1 cut(s) 3456
BseLI CCNNNNNNNGG 9 cut(s) 307, 495, 791, 1545, 1765, 2287, 3062, 3760, 4429
BseMI GCAATG 4 cut(s) 825, 1713, 2079, 2868
BseNI ACTGG 9 cut(s) 421, 689, 1932, 1950, 3415, 3487, 3807, 3863, 4326
BsePI GCGCGC 1 cut(s) 646
BseRI GAGGAG 4 cut(s) 273, 1009, 1085, 3938
BseYI CCCAGC 2 cut(s) 440, 3573
BsgI GTGCAG 1 cut(s) 2851
Bsh1236I CGCG 2 cut(s) 648, 650
BshFI GGCC 6 cut(s) 247, 439, 1012, 1076, 1377, 3099
BshNI GGYRCC 2 cut(s) 950, 958
BsiHKAI GWGCWC 1 cut(s) 1054
BsiHKCI CYCGRG 4 cut(s) 1053, 2318, 2727, 4423
BsiSI CCGG 6 cut(s) 318, 1288, 1405, 1951, 2046, 3775
BslFI GGGAC 5 cut(s) 7, 40, 1421, 3556, 4030
BslI CCNNNNNNNGG 9 cut(s) 307, 495, 791, 1545, 1765, 2287, 3062, 3760, 4429
BsmAI GTCTC 7 cut(s) 920, 944, 1688, 1821, 2315, 3993, 4294
BsmBI CGTCTC 1 cut(s) 944
BsmFI GGGAC 5 cut(s) 7, 40, 1421, 3556, 4030
BsmI GAATGC 6 cut(s) 703, 711, 1858, 1895, 2361, 2500
BsnI GGCC 6 cut(s) 247, 439, 1012, 1076, 1377, 3099
Bso31I GGTCTC 1 cut(s) 4294
BsoBI CYCGRG 4 cut(s) 1053, 2318, 2727, 4423
Bsp1286I GDGCHC 1 cut(s) 1054
Bsp1407I TGTACA 1 cut(s) 810
Bsp1720I GCTNAGC 2 cut(s) 780, 1182
Bsp19I CCATGG 1 cut(s) 954
BspACI CCGC 5 cut(s) 1044, 1777, 2517, 2536, 2885
BspANI GGCC 6 cut(s) 247, 439, 1012, 1076, 1377, 3099
BspFNI CGCG 2 cut(s) 648, 650
BspLI GGNNCC 5 cut(s) 952, 960, 2050, 3105, 3525
BspMAI CTGCAG 1 cut(s) 3044
BspPI GGATC 2 cut(s) 3171, 3222
BspQI GCTCTTC 2 cut(s) 629, 4510
BspT107I GGYRCC 2 cut(s) 950, 958
BspTI CTTAAG 2 cut(s) 479, 4243
BspTNI GGTCTC 1 cut(s) 4294
BsrBI CCGCTC 1 cut(s) 2536
BsrDI GCAATG 4 cut(s) 825, 1713, 2079, 2868
BsrGI TGTACA 1 cut(s) 810
BsrI ACTGG 9 cut(s) 421, 689, 1932, 1950, 3415, 3487, 3807, 3863, 4326
BssECI CCNNGG 9 cut(s) 24, 450, 489, 774, 954, 1468, 2115, 2136, 3969
BssHII GCGCGC 1 cut(s) 646
BssNAI GTATAC 2 cut(s) 1087, 2971
BssT1I CCWWGG 6 cut(s) 489, 954, 1468, 2115, 2136, 3969
Bst1107I GTATAC 2 cut(s) 1087, 2971
Bst2UI CCWGG 8 cut(s) 26, 451, 746, 775, 806, 1721, 2660, 3101
Bst6I CTCTTC 7 cut(s) 629, 1296, 1548, 1873, 2874, 3180, 4510
BstAFI CTTAAG 2 cut(s) 479, 4243
BstAPI GCANNNNNTGC 1 cut(s) 868
BstAUI TGTACA 1 cut(s) 810
BstBAI YACGTR 2 cut(s) 1446, 3614
BstDSI CCRYGG 1 cut(s) 954
BstENI CCTNNNNNAGG 1 cut(s) 1763
BstF5I GGATG 6 cut(s) 319, 577, 2857, 3209, 3965, 4241
BstFNI CGCG 2 cut(s) 648, 650
BstHHI GCGC 3 cut(s) 648, 650, 4195
BstMAI GTCTC 7 cut(s) 920, 944, 1688, 1821, 2315, 3993, 4294
BstMWI GCNNNNNNNGC 8 cut(s) 645, 698, 868, 1378, 2117, 2516, 3893, 4322
BstNI CCWGG 8 cut(s) 26, 451, 746, 775, 806, 1721, 2660, 3101
BstNSI RCATGY 5 cut(s) 1363, 2013, 2042, 3549, 4467
BstPAI GACNNNNGTC 1 cut(s) 3687
BstSFI CTRYAG 4 cut(s) 217, 3040, 3436, 3952
BstSNI TACGTA 1 cut(s) 1446
BstUI CGCG 2 cut(s) 648, 650
BstV2I GAAGAC 1 cut(s) 3720
BstX2I RGATCY 1 cut(s) 3418
BstXI CCANNNNNNTGG 1 cut(s) 1364
BstYI RGATCY 1 cut(s) 3418
BstZ17I GTATAC 2 cut(s) 1087, 2971
Bsu36I CCTNAGG 1 cut(s) 3372
BsuI GTATCC 1 cut(s) 3418
BsuRI GGCC 6 cut(s) 247, 439, 1012, 1076, 1377, 3099
BtgI CCRYGG 1 cut(s) 954
BtrI CACGTC 1 cut(s) 4250
BtsCI GGATG 6 cut(s) 319, 577, 2857, 3209, 3965, 4241
BtsI GCAGTG 1 cut(s) 1236
BtsIMutI CAGTG 9 cut(s) 597, 682, 1236, 1449, 1531, 1939, 2481, 3480, 3685
CaiI CAGNNNCTG 2 cut(s) 1496, 3100
CfoI GCGC 3 cut(s) 648, 650, 4195
Cfr13I GGNCC 8 cut(s) 438, 1375, 1686, 1948, 2573, 3097, 3751, 4174
DraI TTTAAA 2 cut(s) 2683, 4270
DraIII CACNNNGTG 3 cut(s) 842, 3614, 4109
DrdI GACNNNNNNGTC 1 cut(s) 1110
DseDI GACNNNNNNGTC 1 cut(s) 1110
Eam1104I CTCTTC 7 cut(s) 629, 1296, 1548, 1873, 2874, 3180, 4510
EarI CTCTTC 7 cut(s) 629, 1296, 1548, 1873, 2874, 3180, 4510
Eco105I TACGTA 1 cut(s) 1446
Eco130I CCWWGG 6 cut(s) 489, 954, 1468, 2115, 2136, 3969
Eco31I GGTCTC 1 cut(s) 4294
Eco32I GATATC 2 cut(s) 1675, 4369
Eco47I GGWCC 5 cut(s) 1686, 1948, 2573, 3751, 4174
Eco81I CCTNAGG 1 cut(s) 3372
Eco88I CYCGRG 4 cut(s) 1053, 2318, 2727, 4423
EcoNI CCTNNNNNAGG 1 cut(s) 1763
EcoO109I RGGNCCY 2 cut(s) 3097, 3751
EcoRI GAATTC 3 cut(s) 1428, 2914, 3486
EcoRII CCWGG 8 cut(s) 24, 449, 744, 773, 804, 1719, 2658, 3099
EcoRV GATATC 2 cut(s) 1675, 4369
EcoT14I CCWWGG 6 cut(s) 489, 954, 1468, 2115, 2136, 3969
EcoT22I ATGCAT 2 cut(s) 3081, 3967
ErhI CCWWGG 6 cut(s) 489, 954, 1468, 2115, 2136, 3969
Esp3I CGTCTC 1 cut(s) 944
FalI AAGNNNNNCTT 7 cut(s) 2349, 2381, 2979, 3011, 4309, 4341, 4504
FaqI GGGAC 5 cut(s) 7, 40, 1421, 3556, 4030
FauI CCCGC 3 cut(s) 1770, 2529, 2878
FauNDI CATATG 1 cut(s) 3766
FbaI TGATCA 4 cut(s) 1384, 1897, 2817, 3922
FblI GTMKAC 6 cut(s) 1086, 1996, 2970, 3052, 3681, 4114
FokI GGATG 6 cut(s) 326, 584, 2864, 3216, 3952, 4248
FspBI CTAG 6 cut(s) 147, 186, 1652, 2541, 3692, 3701
GlaI GCGC 3 cut(s) 647, 649, 4194
GsaI CCCAGC 2 cut(s) 444, 3577
GsuI CTGGAG 2 cut(s) 3542, 3767
HaeIII GGCC 6 cut(s) 247, 439, 1012, 1076, 1377, 3099
HapII CCGG 6 cut(s) 318, 1288, 1405, 1951, 2046, 3775
HhaI GCGC 3 cut(s) 648, 650, 4195
Hin6I GCGC 3 cut(s) 646, 648, 4193
HinP1I GCGC 3 cut(s) 646, 648, 4193
HincII GTYRAC 2 cut(s) 1115, 4420
HindII GTYRAC 2 cut(s) 1115, 4420
HindIII AAGCTT 6 cut(s) 750, 2220, 2508, 3127, 4286, 4448
HpaII CCGG 6 cut(s) 318, 1288, 1405, 1951, 2046, 3775
HpyCH4IV ACGT 4 cut(s) 1445, 3428, 3613, 4249
HpyF10VI GCNNNNNNNGC 8 cut(s) 645, 698, 868, 1378, 2117, 2516, 3893, 4322
HpySE526I ACGT 4 cut(s) 1445, 3428, 3613, 4249
HspAI GCGC 3 cut(s) 646, 648, 4193
KpnI GGTACC 1 cut(s) 962
Ksp22I TGATCA 4 cut(s) 1384, 1897, 2817, 3922
LguI GCTCTTC 2 cut(s) 629, 4510
LmnI GCTCC 2 cut(s) 2047, 3523
MaeI CTAG 6 cut(s) 147, 186, 1652, 2541, 3692, 3701
MaeII ACGT 4 cut(s) 1445, 3428, 3613, 4249
MaeIII GTNAC 7 cut(s) 179, 1231, 1441, 2735, 3406, 3429, 3663
MbiI CCGCTC 1 cut(s) 2536
MflI RGATCY 1 cut(s) 3418
MhlI GDGCHC 1 cut(s) 1054
MmeI TCCRAC 7 cut(s) 633, 1500, 1713, 2076, 2616, 3141, 3823
Mph1103I ATGCAT 2 cut(s) 3081, 3967
MslI CAYNNNNRTG 4 cut(s) 14, 687, 1362, 1415
MspA1I CMGCKG 1 cut(s) 3887
MspCI CTTAAG 2 cut(s) 479, 4243
MspI CCGG 6 cut(s) 318, 1288, 1405, 1951, 2046, 3775
Mva1269I GAATGC 6 cut(s) 703, 711, 1858, 1895, 2361, 2500
MvaI CCWGG 8 cut(s) 26, 451, 746, 775, 806, 1721, 2660, 3101
MvnI CGCG 2 cut(s) 648, 650
MwoI GCNNNNNNNGC 8 cut(s) 645, 698, 868, 1378, 2117, 2516, 3893, 4322
NciI CCSGG 6 cut(s) 319, 1289, 1406, 1952, 2046, 3776
NcoI CCATGG 1 cut(s) 954
NdeI CATATG 1 cut(s) 3766
NlaIV GGNNCC 5 cut(s) 952, 960, 2050, 3105, 3525
NmuCI GTSAC 2 cut(s) 1231, 3663
NsiI ATGCAT 2 cut(s) 3081, 3967
NspI RCATGY 5 cut(s) 1363, 2013, 2042, 3549, 4467
OliI CACNNNNGTG 1 cut(s) 14
PaeR7I CTCGAG 1 cut(s) 2727
PauI GCGCGC 1 cut(s) 646
PciI ACATGT 2 cut(s) 1359, 4463
PciSI GCTCTTC 2 cut(s) 629, 4510
PctI GAATGC 6 cut(s) 703, 711, 1858, 1895, 2361, 2500
PfeI GAWTC 6 cut(s) 341, 1058, 1463, 2185, 2247, 4208
PflFI GACNNNGTC 1 cut(s) 4251
PflMI CCANNNNNTGG 2 cut(s) 495, 2287
PfoI TCCNGGA 2 cut(s) 1287, 2658
Ppu21I YACGTR 2 cut(s) 1446, 3614
PpuMI RGGWCCY 1 cut(s) 3751
PscI ACATGT 2 cut(s) 1359, 4463
PshAI GACNNNNGTC 1 cut(s) 3687
PshBI ATTAAT 1 cut(s) 3026
PsiI TTATAA 3 cut(s) 1040, 1134, 3477
Psp5II RGGWCCY 1 cut(s) 3751
Psp6I CCWGG 8 cut(s) 24, 449, 744, 773, 804, 1719, 2658, 3099
PspFI CCCAGC 2 cut(s) 440, 3573
PspGI CCWGG 8 cut(s) 24, 449, 744, 773, 804, 1719, 2658, 3099
PspN4I GGNNCC 5 cut(s) 952, 960, 2050, 3105, 3525
PspPI GGNCC 8 cut(s) 438, 1375, 1686, 1948, 2573, 3097, 3751, 4174
PspPPI RGGWCCY 1 cut(s) 3751
PspXI VCTCGAGB 1 cut(s) 2727
PstI CTGCAG 1 cut(s) 3044
PstNI CAGNNNCTG 2 cut(s) 1496, 3100
PsuI RGATCY 1 cut(s) 3418
PsyI GACNNNGTC 1 cut(s) 4251
PteI GCGCGC 1 cut(s) 646
PvuII CAGCTG 1 cut(s) 3887
RseI CAYNNNNRTG 4 cut(s) 14, 687, 1362, 1415
SapI GCTCTTC 2 cut(s) 629, 4510
Sau96I GGNCC 8 cut(s) 438, 1375, 1686, 1948, 2573, 3097, 3751, 4174
SduI GDGCHC 1 cut(s) 1054
SfcI CTRYAG 4 cut(s) 217, 3040, 3436, 3952
Sfr274I CTCGAG 1 cut(s) 2727
SinI GGWCC 5 cut(s) 1686, 1948, 2573, 3751, 4174
SlaI CTCGAG 1 cut(s) 2727
SmiMI CAYNNNNRTG 4 cut(s) 14, 687, 1362, 1415
SmlI CTYRAG 6 cut(s) 129, 479, 1602, 2727, 4178, 4243
SmoI CTYRAG 6 cut(s) 129, 479, 1602, 2727, 4178, 4243
SnaBI TACGTA 1 cut(s) 1446
SpeI ACTAGT 1 cut(s) 185
SsiI CCGC 5 cut(s) 1044, 1777, 2517, 2536, 2885
SspMI CTAG 6 cut(s) 147, 186, 1652, 2541, 3692, 3701
StyI CCWWGG 6 cut(s) 489, 954, 1468, 2115, 2136, 3969
TaiI ACGT 4 cut(s) 1448, 3431, 3616, 4252
TaqI TCGA 6 cut(s) 326, 1172, 1426, 2728, 3960, 4371
TaqII GACCGA 1 cut(s) 3207
TatI WGTACW 6 cut(s) 583, 810, 2165, 3036, 4026, 4049
TauI GCSGC 2 cut(s) 1047, 2519
TfiI GAWTC 6 cut(s) 341, 1058, 1463, 2185, 2247, 4208
TscAI CASTG 9 cut(s) 604, 689, 1236, 1456, 1531, 1939, 2481, 3487, 3692
TseFI GTSAC 2 cut(s) 1231, 3663
Tsp45I GTSAC 2 cut(s) 1231, 3663
TspRI CASTG 9 cut(s) 604, 689, 1236, 1456, 1531, 1939, 2481, 3487, 3692
Tth111I GACNNNGTC 1 cut(s) 4251
Van91I CCANNNNNTGG 2 cut(s) 495, 2287
Vha464I CTTAAG 2 cut(s) 479, 4243
VpaK11BI GGWCC 5 cut(s) 1686, 1948, 2573, 3751, 4174
VspI ATTAAT 1 cut(s) 3026
XagI CCTNNNNNAGG 1 cut(s) 1763
XapI RAATTY 8 cut(s) 673, 964, 1428, 2102, 2914, 3253, 3486, 4310
XbaI TCTAGA 1 cut(s) 3691
XceI RCATGY 5 cut(s) 1363, 2013, 2042, 3549, 4467
XhoI CTCGAG 1 cut(s) 2727
XmiI GTMKAC 6 cut(s) 1086, 1996, 2970, 3052, 3681, 4114
XspI CTAG 6 cut(s) 147, 186, 1652, 2541, 3692, 3701
Zsp2I ATGCAT 2 cut(s) 3081, 3967
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.