RchiOBHm_Chr4g0444011

Glutelin type-A

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0015562
Physical Location & Seq
Reverse (-)
64907711 .. 64908670
960 bp
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UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 507 bp
ATGCTCTGTGAATGCGACATTAGAGCTGCCAAGCTCTCTCTAGAGAATGATGGCTTTGCTCTTCCCAAGTACTCCGACTCTACCAGAGTTGCTTATGTTCTTCAAGGTAATGGAGTAGTCGGAATTTTTTTGCCGAAGAAGGAAGAAAAGGTTCTTCCACTGAATAAGGGTGATGCTATTGCCCTCCTTTTTGGAGTTATCACTTCATGGCACAACAAGGAGGACACTGAGTTTGTGGTTCTTTTCTTGGGTGAAACCAAAACATCTCACAAAGAGGCTCCTCTTGATGTTGATATTAATGGTGGTGGAATAGTTATTCTTTTGAATACTAAGAACCTTCATTTGGTGGGAAAGGGCAGTGGCAGTGTCCAAGTTGTTGGTGTTGATGGGAAGAGGGTATTCGAAACAACCGTGCTTGAAGTTTTCTTCAATGTGGATTTAGATACTGAGAAACTCTTTCGATCAAAGAGAACTTCTGATGCAATTTTCTTTCCTTCTTCGAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cupin_1

Protein Analysis

168

Amino Acids

18.39

Weight (kDa)

5.93

Isoelectric Point (pI)

31.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cupin_1 PF00190 4 - 92 5.2e-12 Cupin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcsI RAATTY 1 cut(s) 123
AfaI GTAC 1 cut(s) 71
AfiI CCNNNNNNNGG 1 cut(s) 343
AgsI TTSAA 4 cut(s) 104, 325, 419, 430
AjuI GAANNNNNNNTTGG 2 cut(s) 326, 358
AluBI AGCT 2 cut(s) 26, 34
AluI AGCT 2 cut(s) 26, 34
ApeKI GCWGC 1 cut(s) 26
ApoI RAATTY 1 cut(s) 123
ArsI GACNNNNNNTTYG 2 cut(s) 215, 247
AseI ATTAAT 1 cut(s) 297
Asp700I GAANNNNTTC 1 cut(s) 150
AsuHPI GGTGA 2 cut(s) 182, 263
AsuII TTCGAA 2 cut(s) 402, 500
BbvI GCAGC 1 cut(s) 13
BccI CCATC 2 cut(s) 44, 380
BfaI CTAG 1 cut(s) 41
BisI GCNGC 1 cut(s) 27
BlsI GCNGC 1 cut(s) 28
BmcAI AGTACT 1 cut(s) 71
BmiI GGNNCC 1 cut(s) 279
BmsI GCATC 2 cut(s) 163, 469
Bpu14I TTCGAA 2 cut(s) 402, 500
Bsc4I CCNNNNNNNGG 1 cut(s) 343
BseLI CCNNNNNNNGG 1 cut(s) 343
BseMII CTCAG 2 cut(s) 219, 438
BseRI GAGGAG 1 cut(s) 270
BseXI GCAGC 1 cut(s) 13
BslI CCNNNNNNNGG 1 cut(s) 343
BsmI GAATGC 1 cut(s) 17
Bsp119I TTCGAA 2 cut(s) 402, 500
Bsp143I GATC 1 cut(s) 461
BspCNI CTCAG 2 cut(s) 220, 439
BspLI GGNNCC 1 cut(s) 279
BspQI GCTCTTC 1 cut(s) 66
BspT104I TTCGAA 2 cut(s) 402, 500
BssMI GATC 1 cut(s) 461
Bst4CI ACNGT 1 cut(s) 412
Bst6I CTCTTC 2 cut(s) 66, 386
BstBI TTCGAA 2 cut(s) 402, 500
BstDEI CTNAG 3 cut(s) 228, 330, 447
BstKTI GATC 1 cut(s) 464
BstMBI GATC 1 cut(s) 461
BstV1I GCAGC 1 cut(s) 13
BstXI CCANNNNNNTGG 1 cut(s) 377
BtsI GCAGTG 2 cut(s) 364, 370
BtsIMutI CAGTG 4 cut(s) 158, 225, 364, 370
Csp6I GTAC 1 cut(s) 70
CviAII CATG 1 cut(s) 207
CviJI RGCY 4 cut(s) 26, 34, 54, 278
CviKI_1 RGCY 4 cut(s) 26, 34, 54, 278
CviQI GTAC 1 cut(s) 70
DdeI CTNAG 3 cut(s) 228, 330, 447
DpnI GATC 1 cut(s) 463
DpnII GATC 1 cut(s) 461
Eam1104I CTCTTC 2 cut(s) 66, 386
EarI CTCTTC 2 cut(s) 66, 386
FaeI CATG 1 cut(s) 210
FaiI YATR 2 cut(s) 96, 208
FatI CATG 1 cut(s) 206
Fnu4HI GCNGC 1 cut(s) 27
Fsp4HI GCNGC 1 cut(s) 27
FspBI CTAG 1 cut(s) 41
GluI GCNGC 1 cut(s) 27
Hin1II CATG 1 cut(s) 210
HinfI GANTC 1 cut(s) 77
HphI GGTGA 2 cut(s) 182, 263
Hpy188I TCNGA 3 cut(s) 76, 122, 478
Hpy188III TCNNGA 2 cut(s) 41, 284
HpyAV CCTTC 3 cut(s) 133, 347, 504
HpyCH4III ACNGT 1 cut(s) 412
HpyCH4V TGCA 1 cut(s) 482
HpyF3I CTNAG 3 cut(s) 228, 330, 447
Hsp92II CATG 1 cut(s) 210
Kzo9I GATC 1 cut(s) 461
LguI GCTCTTC 1 cut(s) 66
LmnI GCTCC 1 cut(s) 283
LpnPI CCDG 1 cut(s) 97
Lsp1109I GCAGC 1 cut(s) 13
LweI GCATC 2 cut(s) 163, 469
MaeI CTAG 1 cut(s) 41
MalI GATC 1 cut(s) 463
MboI GATC 1 cut(s) 461
MboII GAAGA 8 cut(s) 53, 92, 146, 148, 155, 403, 418, 489
MluCI AATT 2 cut(s) 123, 483
MlyI GAGTC 1 cut(s) 71
MmeI TCCRAC 2 cut(s) 99, 100
MnlI CCTC 5 cut(s) 194, 214, 268, 291, 387
MroXI GAANNNNTTC 1 cut(s) 150
MseI TTAA 1 cut(s) 297
Mva1269I GAATGC 1 cut(s) 17
NdeII GATC 1 cut(s) 461
NlaIII CATG 1 cut(s) 210
NlaIV GGNNCC 1 cut(s) 279
NspV TTCGAA 2 cut(s) 402, 500
PciSI GCTCTTC 1 cut(s) 66
PcsI WCGNNNNNNNCGW 1 cut(s) 408
PctI GAATGC 1 cut(s) 17
PdmI GAANNNNTTC 1 cut(s) 150
PkrI GCNGC 1 cut(s) 28
PleI GAGTC 1 cut(s) 71
PpsI GAGTC 1 cut(s) 71
PshBI ATTAAT 1 cut(s) 297
PspN4I GGNNCC 1 cut(s) 279
RsaI GTAC 1 cut(s) 71
RsaNI GTAC 1 cut(s) 70
SapI GCTCTTC 1 cut(s) 66
SaqAI TTAA 1 cut(s) 297
SatI GCNGC 1 cut(s) 27
Sau3AI GATC 1 cut(s) 461
ScaI AGTACT 1 cut(s) 71
SchI GAGTC 1 cut(s) 71
SetI ASST 5 cut(s) 28, 36, 109, 153, 339
SfaNI GCATC 2 cut(s) 163, 469
SfuI TTCGAA 2 cut(s) 402, 500
Sse9I AATT 2 cut(s) 123, 483
SspMI CTAG 1 cut(s) 41
TaaI ACNGT 1 cut(s) 412
TaqI TCGA 3 cut(s) 402, 460, 500
TasI AATT 2 cut(s) 123, 483
TatI WGTACW 1 cut(s) 69
Tru1I TTAA 1 cut(s) 297
Tru9I TTAA 1 cut(s) 297
TscAI CASTG 4 cut(s) 165, 232, 364, 370
TseI GCWGC 1 cut(s) 26
TspDTI ATGAA 2 cut(s) 195, 329
TspRI CASTG 4 cut(s) 165, 232, 364, 370
VspI ATTAAT 1 cut(s) 297
XapI RAATTY 1 cut(s) 123
XbaI TCTAGA 1 cut(s) 40
XmnI GAANNNNTTC 1 cut(s) 150
XspI CTAG 1 cut(s) 41
ZrmI AGTACT 1 cut(s) 71
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.