Rorug07G0020800

Glutelin type-A

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000007
OG0015562
Physical Location & Seq
Reverse (-)
1535584 .. 1543655
8072 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug07G0020800.1

Sequence Viewer

Length: 3693 bp
ATGCCTTACTCCGCCTTCTTTCACCGCTCTATAATAATCCGCACCACCTCCACCGCCATGTCCCTCAAACCCCGCCACCACCACCACCACTTCTCCCTCCTCCGGCGGCCGTCGCTCCTCCTCGGATGCTTCATGAACGCCAACGGCCACTACCACACTCACCCCGTCACCGGCCTGCTCAAATGGAATTCCGCCGGCTGCTGCCGTCACGCTGACGTCACGTGCCTGGCCTCGGCCTCGGCCTCGTCCTCGTCGCGTGCGGCGGAGAGGACGGATTGGAAGCAGCGGCGGAGGAGCGTTGCGGCCGTGCCGGCGTGGTACCAGCAGCAGCAGAAGCAGGGGTATGGGAGGTTTGCGTACCAGGACAACGAGTCGGGTAGTGAAGATTCGGACCGGGAGTGCGGGTCGGGTCAGAGCTCTCGCCGGGAAATGACTGGTTCGACCCTTGACAACATCGATGAGTGGAGATGGAAGTTAACCATGCTTGTTCGCAACAAAGATGAGCAAGAAGTGGTGTCGAGGGAAAGGAAGGACAGGCGTGATTTTGATCATCTTTCAGAACTGGCAAGAGGGATGGGTTTATATAGCCGTCAGTATTCAAAGGTCGTAGTCTTTAGTAAAGTCCCTCAGCCAAATTACAGACCTGATCTGGATGATAGGCGACCACAAAGAGAGGTTGTCTTACCTTTTGGACTGCATAAAGATGTAGATGCTCATCTCAAAGCTCATCTTTCTCAAAAGCCAATGAGCAGAGGAAATTTATCGCATACTTCAATGTCAAGGTCAAGTAGTAATGGAAGTATAGCTAAAGACGGGGGACTCTACGAGCAGGAGGAGCCCTTGATACAGAATAGTGTTGCCATGGAGAGAATTCTTCAGCAGAAAAGCTTGCGATTGCAAAATAAGCAACAAGAATGGCAGGAATCCATCGAAGGTCAAAAGATGCTTGAACTTCGTAGAAGTCTGCCTGCATATAAAGAGAAAGATTCCTTGTTAAAAGCCATATCCGAAAATCAGGTCATAGTTGTCTCAGGTGAAACAGGTTGTGGTAAAACTACACAACTTCCTCAGTACATTTTAGAATCTGAGATTGAAGCTGGTCTTGGAGGTGTCTGTAGTATTATTTGTACTCAGCCTAGACGAATATCTGCCATGTCTGTTTCTGAAAGAGTTGCTGCAGAACGTGGGGAGAATCTGGGGGAATCAGTTGGTTACAAAGTTAGGCTGGAAGGAATGAAAGGGAGGGACACGCGGCTTCTTTTTTGCACCACAGGCATACTATTGAGAAGATTACTTGTTGACAGAAAGTTGAGAGGTGTTACACATGTCATTGTTGATGAGATTCATGAACGTGGAATGAATGAAGATTTTCTTCTTATCGTTCTGAAAGAACTTCTCTCTCGTCGTCCTGAGTTGAGATTGATTTTGATGAGTGCAACCTTAAATGCTGAGCTTTTCTCCTCATACTTTAATGGTGCTCCAATGATCCATATACCTGGTTTTACATACCCGGTCCGAGCATATTTTCTGGAGAATATTCTAGAAATGACTGGATATCGGTTAAATCAATATAATCAAATTGATGATTATGGTCAAGATAAGACGTGGAAAATGCAGAAACAAGCTCAAGCTTTCAAAAAGAGGAAGAGCCAAATTGCTTCTACCGTTGAGGATGCACTTGAAGCTGCTGACTTTAGGGAGTACAGTCCAAGGACTCAGGAGTCTCTGTCCTGTTGGAATCCGGACTCCATTGGTTTTAATCTCATTGAGCATGTGCTTTGCCACATAGTCAGGAAAGAACGGCCTGGTGCTATTTTGGTTTTTATGACTGGTTGGGATGACATAAATTCCTTGAAGGATCAACTCCAATCTCATCCCCTTTTAGGAGATTCTAGTAGGGTCCTGCTGCTTGCCTGTCATGGTTCCATGCCTAGCTCTGAACAGAGGTTGATATTTGATAAACCAGAAGATGGAGTTAGGAAAATCGTTCTGGCTACAAACATGGCTGAGACCAGTATCACTATCAATGATGTAGTATTTGTGATTGATTGTGGAAAGGCGAAAGAAACATCATATGATGCACTCAATAATACTCCCTGTTTGCTTCCATCCTGGATATCAAAGGCTGCTTCCCGGCAAAGAAGAGGAAGAGCCGGTCGTGTTCAACCTGGCGAGTGTTACCATCTATATCCCCGATGTGTATATGATGCCTTTGCTGATTATCAATTGCCTGAACTTTTGAGGACGCCTCTGCAGTCACTATGTTTACAAATCAAGAGTCTACAACTTGGAAGTATTTCTGAGTTCCTTTCTAAGGCACTGCAGTCACCGGAACCTTTGTCGGTTCAAAATGCTGTTGATTATCTGAAGATCATTGGGGCTTTAGATGATAATGAAGATCTCACAGTATTAGGTCGCCACCTGTCAATGCTTCCGGTTGAGCCAAAACTTGGGAAAATGCTCATATTAGGGGCTATCTTCAATTGTTTAGATCCAATAATGACTATTGTTGCAGGCCTTAGTATGAGAGATCCATTCATGATGCCATTTGACAAGAAGGATCTTGCAGAATCGGCAAAAGCACAATTTTCTGGCCGTGATAGCAGTGATCATCTGGCTCTTATCCGAGCATATGATGGTTGGAAAAATGCTGAAAGATCACAAGCTGGTTATGAGTACTGCTGGAGAAATTTTCTTTCTGCACAAACTCTAAAAGCCATTGACTCTCTTCGGAAGCAGTTCTTCTTCTTGCTCAAGGATGCTGGTTTGGTTGATCACAACACCGAGAACTGCAATACATTGAGCCATGATGAACATCTTATCCGAGCAATCATCTGTGCAGGATTATTTCCTGGAATATGTTCTGTTGTGAACAAAGAGAAGTCGATTTCGTTGAAAACAATGGAAGATGGACAAGTACTTCTGTACTCGAATTCTGTCAATGCTTCAGTGCCTAAAATTCCATATCCATGGCTAGTTTTCAACGAAAAGGTGAAAGTGAATTCAGTATTCCTCCGGGATTCAACCGGCGTATCTGATTCTGTGCTCCTTTTGTTTGGAGGCAACATTTCCAGGGGTGGACTGGATGGGCACCTGAAGATGTTGGGAGGATACTTGGAGTTTTTCATGAATCCTGCATTAGCAAATACATATGTTAGCTTAAAGAGGGAACTTGAGGAACTGATTCATAATAAGCTTATAGATCCCAAATTGGATATGCAATCTCATACTAACCTCCTATCAGCCCTAAGATTACTGGTCTCTGAAGACCGATGTGATGGTAGATTTGTCTTTGGTCGCAAGATGCCAGTACCCTCAAAGAAGATAACAAAAGAGTTAGGTCCAGGCACCTTGAGGGTTGGCGATAATGGTAAAAGCGGGGGTAACAATTCCAAAAGTCAGCTGCAAACATTGCTTGTCAGAGCAGGACATGAAGCTCCCACCTACAAAACAAAACAACTGAAAAACAACCAGTTTCGGTCCACAGTGATCTTCAATGGGTTGAACTTTGTAGGGCAGCCTTCCAATAGTAAGAAACAAGCAGAGAAGGAAGCGGCTGCTGAAGCTGTGCTCTGGTTAAAGGGCGATAACCATTCATCTTCCACAGATATTGACCATATGTCAATGCTTCTAAAGAAGAGCAGAAAGAAAATCCATAAAACGACCTCATTTGATAGCGCTAAGTGGAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cupin_1

Protein Analysis

1230

Amino Acids

138.58

Weight (kDa)

9.01

Isoelectric Point (pI)

51.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DEAD PF00270 331 - 484 4.1e-10 DEAD/DEAH box helicase
Helicase_C PF00271 592 - 721 6.2e-13 Helicase conserved C-terminal domain
WHD_HA2 PF04408 785 - 813 3.3e-08 Helicase associated domain (HA2), winged-helix
HA2_C PF21010 814 - 874 1.8e-14 Helicase associated domain (HA2), ratchet-like
OB_NTP_bind PF07717 941 - 1023 9.9e-20 Oligonucleotide/oligosaccharide-binding (OB)-fold
RNA_hel_CTD PF26026 1031 - 1089 2.1e-18 C-terminal domain in RNA helicases
dsrm PF00035 1132 - 1193 1.1e-08 Double-stranded RNA binding motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1720
AatII GACGTC 1 cut(s) 219
Acc65I GGTACC 1 cut(s) 318
AccB1I GGYRCC 3 cut(s) 318, 3090, 3347
AccB7I CCANNNNNTGG 2 cut(s) 1970, 2450
AccBSI CCGCTC 1 cut(s) 27
AccI GTMKAC 1 cut(s) 2281
AccII CGCG 2 cut(s) 256, 1252
AccIII TCCGGA 1 cut(s) 1741
AclWI GGATC 6 cut(s) 1480, 1866, 2486, 2525, 2568, 3197
AcoI YGGCCR 4 cut(s) 107, 145, 303, 2593
AcsI RAATTY 8 cut(s) 187, 757, 870, 1846, 2689, 2932, 2958, 3001
AcuI CTGAAG 6 cut(s) 860, 2387, 2931, 3116, 3285, 3582
AcvI CACGTG 1 cut(s) 222
AcyI GRCGYC 2 cut(s) 216, 2246
AfaI GTAC 9 cut(s) 320, 359, 1073, 1129, 1703, 2678, 2919, 2927, 3312
AfeI AGCGCT 1 cut(s) 3679
AfiI CCNNNNNNNGG 7 cut(s) 102, 170, 232, 1883, 1970, 2314, 2450
AflIII ACRYGT 1 cut(s) 1324
AhdI GACNNNNNGTC 2 cut(s) 370, 3619
AjiI CACGTC 1 cut(s) 1605
AjnI CCWGG 9 cut(s) 225, 360, 1495, 1804, 2111, 2167, 2851, 3071, 3343
AjuI GAANNNNNNNTTGG 2 cut(s) 1086, 1118
AloI GAACNNNNNNTCC 2 cut(s) 2805, 2837
Alw21I GWGCWC 4 cut(s) 419, 1480, 3048, 3573
Alw26I GTCTC 4 cut(s) 1033, 1728, 2003, 3265
AlwI GGATC 6 cut(s) 1480, 1866, 2486, 2525, 2568, 3197
Aor13HI TCCGGA 1 cut(s) 1741
Aor51HI AGCGCT 1 cut(s) 3679
ApoI RAATTY 8 cut(s) 187, 757, 870, 1846, 2689, 2932, 2958, 3001
ArsI GACNNNNNNTTYG 4 cut(s) 1002, 1034, 2263, 2295
Asp700I GAANNNNTTC 5 cut(s) 1368, 2296, 2738, 2860, 3183
Asp718I GGTACC 1 cut(s) 318
AspLEI GCGC 1 cut(s) 3680
AspS9I GGNCC 5 cut(s) 391, 1513, 1900, 3341, 3480
AsuC2I CCSGG 5 cut(s) 395, 425, 1511, 2134, 3017
AsuHPI GGTGA 6 cut(s) 14, 152, 160, 1046, 2319, 3004
AvaII GGWCC 5 cut(s) 391, 1513, 1900, 3341, 3480
BaeGI GKGCMC 1 cut(s) 3093
BaeI ACNNNNGTAYC 2 cut(s) 3015, 3048
BanI GGYRCC 3 cut(s) 318, 3090, 3347
BanII GRGCYC 2 cut(s) 419, 840
BbrPI CACGTG 1 cut(s) 222
BbsI GAAGAC 1 cut(s) 3273
Bbv12I GWGCWC 4 cut(s) 419, 1480, 3048, 3573
BbvCI CCTCAGC 1 cut(s) 627
BceAI ACGGC 7 cut(s) 94, 160, 189, 290, 573, 1817, 2580
BciT130I CCWGG 9 cut(s) 227, 362, 1497, 1806, 2113, 2169, 2853, 3073, 3345
BciVI GTATCC 1 cut(s) 3104
BclI TGATCA 3 cut(s) 547, 2608, 2773
BcnI CCSGG 5 cut(s) 395, 425, 1511, 2134, 3017
BcoDI GTCTC 4 cut(s) 1033, 1728, 2003, 3265
BfaI CTAG 5 cut(s) 1137, 1541, 1893, 1932, 2975
BfmI CTRYAG 4 cut(s) 1114, 1176, 2252, 2321
BfoI RGCGCY 1 cut(s) 3681
BfuI GTATCC 1 cut(s) 3104
BglI GCCNNNNNGGC 1 cut(s) 311
BglII AGATCT 1 cut(s) 2398
BlpI GCTNAGC 1 cut(s) 1449
BmcAI AGTACT 2 cut(s) 2678, 2919
Bme18I GGWCC 5 cut(s) 391, 1513, 1900, 3341, 3480
BmeRI GACNNNNNGTC 2 cut(s) 370, 3619
BmgBI CACGTC 1 cut(s) 1605
BmgT120I GGNCC 5 cut(s) 391, 1513, 1900, 3341, 3480
BmiI GGNNCC 7 cut(s) 320, 837, 1901, 1924, 2334, 3092, 3349
BmsI GCATC 9 cut(s) 116, 700, 933, 1663, 2068, 2197, 2532, 2749, 3294
BpiI GAAGAC 1 cut(s) 3273
BplI GAGNNNNNCTC 4 cut(s) 1442, 1474, 2233, 2265
BpmI CTGGAG 2 cut(s) 1550, 2704
Bpu10I CCTNAGC 1 cut(s) 627
Bpu1102I GCTNAGC 1 cut(s) 1449
BpuEI CTTGAG 4 cut(s) 1611, 2738, 3194, 3373
BpuMI CCSGG 5 cut(s) 395, 425, 1511, 2134, 3017
Bsa29I ATCGAT 1 cut(s) 456
BsaAI YACGTR 1 cut(s) 222
BsaBI GATNNNNATC 3 cut(s) 546, 714, 2814
BsaHI GRCGYC 2 cut(s) 216, 2246
BsaI GGTCTC 2 cut(s) 2003, 3265
BsaJI CCNNGG 7 cut(s) 121, 231, 237, 861, 1709, 2969, 3072
BsaWI WCCGGW 3 cut(s) 1741, 2329, 2434
BsaXI ACNNNNNCTCC 6 cut(s) 77, 107, 389, 419, 1712, 1742
Bsc4I CCNNNNNNNGG 7 cut(s) 102, 170, 232, 1883, 1970, 2314, 2450
Bse118I RCCGGY 5 cut(s) 170, 194, 310, 2153, 3026
Bse1I ACTGG 9 cut(s) 439, 567, 1555, 1834, 2013, 3087, 3261, 3308, 3472
Bse3DI GCAATG 1 cut(s) 3410
Bse8I GATNNNNATC 3 cut(s) 546, 714, 2814
BseAI TCCGGA 1 cut(s) 1741
BseBI CCWGG 9 cut(s) 227, 362, 1497, 1806, 2113, 2169, 2853, 3073, 3345
BseCI ATCGAT 1 cut(s) 456
BseDI CCNNGG 7 cut(s) 121, 231, 237, 861, 1709, 2969, 3072
BseGI GGATG 9 cut(s) 131, 579, 658, 1678, 1843, 1873, 2108, 2764, 3091
BseJI GATNNNNATC 3 cut(s) 546, 714, 2814
BseLI CCNNNNNNNGG 7 cut(s) 102, 170, 232, 1883, 1970, 2314, 2450
BseMI GCAATG 1 cut(s) 3410
BseNI ACTGG 9 cut(s) 439, 567, 1555, 1834, 2013, 3087, 3261, 3308, 3472
BseRI GAGGAG 6 cut(s) 89, 107, 110, 307, 848, 1450
BseSI GKGCMC 1 cut(s) 3093
BseX3I CGGCCG 2 cut(s) 107, 303
BsgI GTGCAG 2 cut(s) 2685, 2859
Bsh1236I CGCG 2 cut(s) 256, 1252
Bsh1285I CGRYCG 3 cut(s) 110, 306, 2158
BshNI GGYRCC 3 cut(s) 318, 3090, 3347
BshVI ATCGAT 1 cut(s) 456
BsiEI CGRYCG 3 cut(s) 110, 306, 2158
BsiHKAI GWGCWC 4 cut(s) 419, 1480, 3048, 3573
BslFI GGGAC 4 cut(s) 46, 608, 831, 1259
BslI CCNNNNNNNGG 7 cut(s) 102, 170, 232, 1883, 1970, 2314, 2450
BsmAI GTCTC 4 cut(s) 1033, 1728, 2003, 3265
BsmFI GGGAC 4 cut(s) 46, 608, 831, 1259
Bso31I GGTCTC 2 cut(s) 2003, 3265
Bsp1286I GDGCHC 6 cut(s) 419, 840, 1480, 3048, 3093, 3573
Bsp13I TCCGGA 1 cut(s) 1741
Bsp1720I GCTNAGC 1 cut(s) 1449
Bsp19I CCATGG 2 cut(s) 861, 2969
BspDI ATCGAT 1 cut(s) 456
BspEI TCCGGA 1 cut(s) 1741
BspFNI CGCG 2 cut(s) 256, 1252
BspHI TCATGA 4 cut(s) 132, 1345, 2538, 3126
BspLI GGNNCC 7 cut(s) 320, 837, 1901, 1924, 2334, 3092, 3349
BspMAI CTGCAG 3 cut(s) 1180, 2256, 2325
BspPI GGATC 6 cut(s) 1480, 1866, 2486, 2525, 2568, 3197
BspQI GCTCTTC 3 cut(s) 1640, 2143, 3632
BspT107I GGYRCC 3 cut(s) 318, 3090, 3347
BspTNI GGTCTC 2 cut(s) 2003, 3265
BsrBI CCGCTC 1 cut(s) 27
BsrDI GCAATG 1 cut(s) 3410
BsrFI RCCGGY 5 cut(s) 170, 194, 310, 2153, 3026
BsrI ACTGG 9 cut(s) 439, 567, 1555, 1834, 2013, 3087, 3261, 3308, 3472
BssAI RCCGGY 5 cut(s) 170, 194, 310, 2153, 3026
BssECI CCNNGG 7 cut(s) 121, 231, 237, 861, 1709, 2969, 3072
BssNI GRCGYC 2 cut(s) 216, 2246
BssT1I CCWWGG 3 cut(s) 861, 1709, 2969
Bst2UI CCWGG 9 cut(s) 227, 362, 1497, 1806, 2113, 2169, 2853, 3073, 3345
Bst4CI ACNGT 4 cut(s) 1666, 1706, 2407, 3487
Bst6I CTCTTC 5 cut(s) 1640, 2137, 2143, 2733, 3632
BstACI GRCGYC 2 cut(s) 216, 2246
BstAPI GCANNNNNTGC 1 cut(s) 3412
BstBAI YACGTR 1 cut(s) 222
BstC8I GCNNGC 8 cut(s) 176, 196, 258, 312, 890, 969, 1911, 2515
BstDSI CCRYGG 2 cut(s) 861, 2969
BstENI CCTNNNNNAGG 1 cut(s) 2312
BstF5I GGATG 9 cut(s) 131, 579, 658, 1678, 1843, 1873, 2108, 2764, 3091
BstFNI CGCG 2 cut(s) 256, 1252
BstH2I RGCGCY 1 cut(s) 3681
BstHHI GCGC 1 cut(s) 3680
BstMAI GTCTC 4 cut(s) 1033, 1728, 2003, 3265
BstMCI CGRYCG 3 cut(s) 110, 306, 2158
BstNI CCWGG 9 cut(s) 227, 362, 1497, 1806, 2113, 2169, 2853, 3073, 3345
BstNSI RCATGY 2 cut(s) 1328, 1775
BstSFI CTRYAG 4 cut(s) 1114, 1176, 2252, 2321
BstSLI GKGCMC 1 cut(s) 3093
BstUI CGCG 2 cut(s) 256, 1252
BstV2I GAAGAC 1 cut(s) 3273
BstX2I RGATCY 5 cut(s) 2398, 2491, 2530, 2560, 3202
BstXI CCANNNNNNTGG 2 cut(s) 1496, 2970
BstYI RGATCY 5 cut(s) 2398, 2491, 2530, 2560, 3202
BstZI CGGCCG 2 cut(s) 107, 303
Bsu15I ATCGAT 1 cut(s) 456
BsuI GTATCC 1 cut(s) 3104
BsuTUI ATCGAT 1 cut(s) 456
BtgI CCRYGG 2 cut(s) 861, 2969
BtrI CACGTC 1 cut(s) 1605
BtsCI GGATG 9 cut(s) 131, 579, 658, 1678, 1843, 1873, 2108, 2764, 3091
BtsI GCAGTG 2 cut(s) 2318, 2611
BtsIMutI CAGTG 4 cut(s) 2318, 2611, 2955, 3492
Cac8I GCNNGC 8 cut(s) 176, 196, 258, 312, 890, 969, 1911, 2515
CciI TCATGA 4 cut(s) 132, 1345, 2538, 3126
CfoI GCGC 1 cut(s) 3680
Cfr10I RCCGGY 5 cut(s) 170, 194, 310, 2153, 3026
Cfr13I GGNCC 5 cut(s) 391, 1513, 1900, 3341, 3480
ClaI ATCGAT 1 cut(s) 456
CpoI CGGWCCG 2 cut(s) 391, 1513
CseI GACGC 1 cut(s) 2254
CsiI ACCWGGT 1 cut(s) 1495
Csp6I GTAC 9 cut(s) 319, 358, 1072, 1128, 1702, 2677, 2918, 2926, 3311
CspCI CAANNNNNGTGG 2 cut(s) 3592, 3627
CspI CGGWCCG 2 cut(s) 391, 1513
CviQI GTAC 9 cut(s) 319, 358, 1072, 1128, 1702, 2677, 2918, 2926, 3311
DrdI GACNNNNNNGTC 1 cut(s) 1720
DriI GACNNNNNGTC 2 cut(s) 370, 3619
DseDI GACNNNNNNGTC 1 cut(s) 1720
EaeI YGGCCR 4 cut(s) 107, 145, 303, 2593
EagI CGGCCG 2 cut(s) 107, 303
Eam1104I CTCTTC 5 cut(s) 1640, 2137, 2143, 2733, 3632
Eam1105I GACNNNNNGTC 2 cut(s) 370, 3619
EarI CTCTTC 5 cut(s) 1640, 2137, 2143, 2733, 3632
EciI GGCGGA 3 cut(s) 181, 278, 304
Ecl136II GAGCTC 1 cut(s) 417
EclXI CGGCCG 2 cut(s) 107, 303
Eco130I CCWWGG 3 cut(s) 861, 1709, 2969
Eco147I AGGCCT 1 cut(s) 2517
Eco24I GRGCYC 2 cut(s) 419, 840
Eco31I GGTCTC 2 cut(s) 2003, 3265
Eco32I GATATC 2 cut(s) 1556, 2118
Eco47I GGWCC 5 cut(s) 391, 1513, 1900, 3341, 3480
Eco47III AGCGCT 1 cut(s) 3679
Eco52I CGGCCG 2 cut(s) 107, 303
Eco53kI GAGCTC 1 cut(s) 417
Eco57I CTGAAG 6 cut(s) 860, 2387, 2931, 3116, 3285, 3582
Eco72I CACGTG 1 cut(s) 222
EcoICRI GAGCTC 1 cut(s) 417
EcoNI CCTNNNNNAGG 1 cut(s) 2312
EcoO109I RGGNCCY 1 cut(s) 1900
EcoRI GAATTC 4 cut(s) 187, 870, 2932, 3001
EcoRII CCWGG 9 cut(s) 225, 360, 1495, 1804, 2111, 2167, 2851, 3071, 3343
EcoRV GATATC 2 cut(s) 1556, 2118
EcoT14I CCWWGG 3 cut(s) 861, 1709, 2969
EcoT38I GRGCYC 2 cut(s) 419, 840
ErhI CCWWGG 3 cut(s) 861, 1709, 2969
FalI AAGNNNNNCTT 8 cut(s) 714, 746, 1086, 1118, 1356, 1388, 2726, 2758
FaqI GGGAC 4 cut(s) 46, 608, 831, 1259
FauI CCCGC 3 cut(s) 80, 395, 3371
FauNDI CATATG 4 cut(s) 2074, 2632, 3151, 3618
FbaI TGATCA 3 cut(s) 547, 2608, 2773
FblI GTMKAC 1 cut(s) 2281
FokI GGATG 9 cut(s) 138, 586, 665, 1685, 1850, 1860, 2095, 2771, 3098
FriOI GRGCYC 2 cut(s) 419, 840
FspBI CTAG 5 cut(s) 1137, 1541, 1893, 1932, 2975
GlaI GCGC 1 cut(s) 3679
GsuI CTGGAG 2 cut(s) 1550, 2704
HaeII RGCGCY 1 cut(s) 3681
HgaI GACGC 1 cut(s) 2254
HhaI GCGC 1 cut(s) 3680
Hin1I GRCGYC 2 cut(s) 216, 2246
Hin6I GCGC 1 cut(s) 3678
HinP1I GCGC 1 cut(s) 3678
HincII GTYRAC 2 cut(s) 477, 1300
HindII GTYRAC 2 cut(s) 477, 1300
HindIII AAGCTT 3 cut(s) 886, 1629, 3194
HpaI GTTAAC 1 cut(s) 477
HphI GGTGA 6 cut(s) 14, 152, 160, 1046, 2319, 3004
Hpy166II GTNNAC 7 cut(s) 477, 1300, 2267, 2282, 2872, 3080, 3483
Hpy8I GTNNAC 7 cut(s) 477, 1300, 2267, 2282, 2872, 3080, 3483
Hpy99I CGWCG 3 cut(s) 115, 256, 1407
HpyAV CCTTC 8 cut(s) 25, 523, 926, 1223, 1849, 2551, 3531, 3541
HpyCH4III ACNGT 4 cut(s) 1666, 1706, 2407, 3487
HpyCH4IV ACGT 5 cut(s) 216, 221, 1183, 1351, 1604
HpySE526I ACGT 5 cut(s) 216, 221, 1183, 1351, 1604
Hsp92I GRCGYC 2 cut(s) 216, 2246
HspAI GCGC 1 cut(s) 3678
Kpn2I TCCGGA 1 cut(s) 1741
KpnI GGTACC 1 cut(s) 322
KroI GCCGGC 2 cut(s) 194, 310
KroNI GCCGGC 2 cut(s) 196, 312
Ksp22I TGATCA 3 cut(s) 547, 2608, 2773
KspAI GTTAAC 1 cut(s) 477
LguI GCTCTTC 3 cut(s) 1640, 2143, 3632
LmnI GCTCC 6 cut(s) 120, 294, 835, 1483, 3051, 3442
LweI GCATC 9 cut(s) 116, 700, 933, 1663, 2068, 2197, 2532, 2749, 3294
MabI ACCWGGT 1 cut(s) 1495
MaeI CTAG 5 cut(s) 1137, 1541, 1893, 1932, 2975
MaeII ACGT 5 cut(s) 216, 221, 1183, 1351, 1604
MaeIII GTNAC 9 cut(s) 166, 206, 217, 1211, 1318, 2177, 2256, 2325, 3383
MbiI CCGCTC 1 cut(s) 27
MfeI CAATTG 2 cut(s) 2225, 2482
MflI RGATCY 5 cut(s) 2398, 2491, 2530, 2560, 3202
MhlI GDGCHC 6 cut(s) 419, 840, 1480, 3048, 3093, 3573
MlyI GAGTC 7 cut(s) 380, 813, 1708, 1730, 1739, 2287, 2717
MmeI TCCRAC 2 cut(s) 1715, 2621
MroI TCCGGA 1 cut(s) 1741
MroNI GCCGGC 2 cut(s) 194, 310
MroXI GAANNNNTTC 5 cut(s) 1368, 2296, 2738, 2860, 3183
MseI TTAA 8 cut(s) 476, 995, 1442, 1470, 1562, 1758, 3161, 3578
MslI CAYNNNNRTG 4 cut(s) 56, 702, 1350, 2968
MspA1I CMGCKG 2 cut(s) 286, 3403
MunI CAATTG 2 cut(s) 2225, 2482
MvaI CCWGG 9 cut(s) 227, 362, 1497, 1806, 2113, 2169, 2853, 3073, 3345
MvnI CGCG 2 cut(s) 256, 1252
NaeI GCCGGC 2 cut(s) 196, 312
NciI CCSGG 5 cut(s) 395, 425, 1511, 2134, 3017
NcoI CCATGG 2 cut(s) 861, 2969
NdeI CATATG 4 cut(s) 2074, 2632, 3151, 3618
NgoMIV GCCGGC 2 cut(s) 194, 310
NlaIV GGNNCC 7 cut(s) 320, 837, 1901, 1924, 2334, 3092, 3349
NmeAIII GCCGAG 2 cut(s) 212, 218
NmuCI GTSAC 5 cut(s) 166, 206, 217, 2256, 2325
NspI RCATGY 2 cut(s) 1328, 1775
PagI TCATGA 4 cut(s) 132, 1345, 2538, 3126
PceI AGGCCT 1 cut(s) 2517
PciI ACATGT 1 cut(s) 1324
PciSI GCTCTTC 3 cut(s) 1640, 2143, 3632
PdiI GCCGGC 2 cut(s) 196, 312
PdmI GAANNNNTTC 5 cut(s) 1368, 2296, 2738, 2860, 3183
PflMI CCANNNNNTGG 2 cut(s) 1970, 2450
PfoI TCCNGGA 3 cut(s) 2111, 2851, 3015
PleI GAGTC 7 cut(s) 379, 813, 1708, 1729, 1739, 2286, 2717
PmaCI CACGTG 1 cut(s) 222
PmlI CACGTG 1 cut(s) 222
PpsI GAGTC 7 cut(s) 379, 813, 1708, 1729, 1739, 2286, 2717
Ppu21I YACGTR 1 cut(s) 222
PpuMI RGGWCCY 1 cut(s) 1900
PscI ACATGT 1 cut(s) 1324
Psp124BI GAGCTC 1 cut(s) 419
Psp5II RGGWCCY 1 cut(s) 1900
Psp6I CCWGG 9 cut(s) 225, 360, 1495, 1804, 2111, 2167, 2851, 3071, 3343
PspCI CACGTG 1 cut(s) 222
PspGI CCWGG 9 cut(s) 225, 360, 1495, 1804, 2111, 2167, 2851, 3071, 3343
PspN4I GGNNCC 7 cut(s) 320, 837, 1901, 1924, 2334, 3092, 3349
PspPI GGNCC 5 cut(s) 391, 1513, 1900, 3341, 3480
PspPPI RGGWCCY 1 cut(s) 1900
PstI CTGCAG 3 cut(s) 1180, 2256, 2325
PsuI RGATCY 5 cut(s) 2398, 2491, 2530, 2560, 3202
PvuII CAGCTG 1 cut(s) 3403
RsaI GTAC 9 cut(s) 320, 359, 1073, 1129, 1703, 2678, 2919, 2927, 3312
RsaNI GTAC 9 cut(s) 319, 358, 1072, 1128, 1702, 2677, 2918, 2926, 3311
RseI CAYNNNNRTG 4 cut(s) 56, 702, 1350, 2968
Rsr2I CGGWCCG 2 cut(s) 391, 1513
RsrII CGGWCCG 2 cut(s) 391, 1513
SacI GAGCTC 1 cut(s) 419
SapI GCTCTTC 3 cut(s) 1640, 2143, 3632
SaqAI TTAA 8 cut(s) 476, 995, 1442, 1470, 1562, 1758, 3161, 3578
Sau96I GGNCC 5 cut(s) 391, 1513, 1900, 3341, 3480
ScaI AGTACT 2 cut(s) 2678, 2919
SchI GAGTC 7 cut(s) 380, 813, 1708, 1730, 1739, 2287, 2717
SduI GDGCHC 6 cut(s) 419, 840, 1480, 3048, 3093, 3573
SexAI ACCWGGT 1 cut(s) 1495
SfaNI GCATC 9 cut(s) 116, 700, 933, 1663, 2068, 2197, 2532, 2749, 3294
SfcI CTRYAG 4 cut(s) 1114, 1176, 2252, 2321
SinI GGWCC 5 cut(s) 391, 1513, 1900, 3341, 3480
SmiMI CAYNNNNRTG 4 cut(s) 56, 702, 1350, 2968
SmlI CTYRAG 4 cut(s) 1626, 2753, 3173, 3352
SmoI CTYRAG 4 cut(s) 1626, 2753, 3173, 3352
SseBI AGGCCT 1 cut(s) 2517
SspI AATATT 1 cut(s) 1537
SspMI CTAG 5 cut(s) 1137, 1541, 1893, 1932, 2975
SstI GAGCTC 1 cut(s) 419
StuI AGGCCT 1 cut(s) 2517
StyI CCWWGG 3 cut(s) 861, 1709, 2969
TaaI ACNGT 4 cut(s) 1666, 1706, 2407, 3487
TaiI ACGT 5 cut(s) 219, 224, 1186, 1354, 1607
TaqI TCGA 6 cut(s) 440, 456, 518, 930, 2885, 2930
TaqII GACCGA 2 cut(s) 3285, 3468
TatI WGTACW 6 cut(s) 1071, 1127, 1701, 2676, 2917, 2925
TauI GCSGC 6 cut(s) 109, 263, 289, 305, 1255, 3557
Tru1I TTAA 8 cut(s) 476, 995, 1442, 1470, 1562, 1758, 3161, 3578
Tru9I TTAA 8 cut(s) 476, 995, 1442, 1470, 1562, 1758, 3161, 3578
TscAI CASTG 4 cut(s) 2325, 2611, 2955, 3492
TseFI GTSAC 5 cut(s) 166, 206, 217, 2256, 2325
Tsp45I GTSAC 5 cut(s) 166, 206, 217, 2256, 2325
TspGWI ACGGA 1 cut(s) 287
TspRI CASTG 4 cut(s) 2325, 2611, 2955, 3492
Van91I CCANNNNNTGG 2 cut(s) 1970, 2450
VpaK11BI GGWCC 5 cut(s) 391, 1513, 1900, 3341, 3480
XagI CCTNNNNNAGG 1 cut(s) 2312
XapI RAATTY 8 cut(s) 187, 757, 870, 1846, 2689, 2932, 2958, 3001
XbaI TCTAGA 1 cut(s) 1540
XceI RCATGY 2 cut(s) 1328, 1775
XcmI CCANNNNNNNNNTGG 1 cut(s) 3079
XmiI GTMKAC 1 cut(s) 2281
XmnI GAANNNNTTC 5 cut(s) 1368, 2296, 2738, 2860, 3183
XspI CTAG 5 cut(s) 1137, 1541, 1893, 1932, 2975
ZraI GACGTC 1 cut(s) 217
ZrmI AGTACT 2 cut(s) 2678, 2919
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.