Rh7AG072700

Glutelin type-A

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7A
OG0015562
Physical Location & Seq
Reverse (-)
5181304 .. 5182334
1031 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7AG072700.1

Sequence Viewer

Length: 507 bp
ATGGAGATTGATCTTACACTAAGGTTGGCTAAGAAGGTTTATGGAGGAGATGGTGGTTCCTACCCTGCCTGGTCTCCGTCGGAGCTTCCCATGCTCCGTGAAGATGACATCGGAGCTGCCCAGCTCTCTCTAGAGAAGGATGGCCTTGCTCTCCCCAATTACTCTGACTTTGCCAGAGTTGCATATGTCCTTCAAGGTAATGGAGTAGTTGGAATTGTTCTGCCGGAGAAGGAAGAAAAGCTCATAAAAAAGGCTAATTCACTTACTTCTATCTCAATGGTTCTAATGACATTTTCACTGGCTTCTCAATTGAGTTTGTTGGCTGAGCATGAGATTTGGAGGAGTGTTGTAAAGACTCTTGTTGGCAAGCAAACCGGCAAGGGCATTGTTAAGTTAGGGGGAGCTAATTTGCCTGAGCCAAACAAGGAACATAGAGATGAGATGACATTGAATTGCGAAGAGGCTCCTCTTGATGTTGATATTAAGGGCGGTGGAAGAATTGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cupin_1

Protein Analysis

168

Amino Acids

18.18

Weight (kDa)

5.27

Isoelectric Point (pI)

32.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cupin_1 PF00190 10 - 84 4e-10 Cupin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 489
AgsI TTSAA 2 cut(s) 194, 451
AjnI CCWGG 1 cut(s) 68
AluBI AGCT 5 cut(s) 85, 116, 124, 241, 404
AluI AGCT 5 cut(s) 85, 116, 124, 241, 404
Alw26I GTCTC 1 cut(s) 78
AoxI GGCC 1 cut(s) 142
ApeKI GCWGC 1 cut(s) 116
BbvI GCAGC 1 cut(s) 103
BccI CCATC 2 cut(s) 44, 134
BciT130I CCWGG 1 cut(s) 70
BcoDI GTCTC 1 cut(s) 78
BfaI CTAG 1 cut(s) 131
BisI GCNGC 1 cut(s) 117
BlpI GCTNAGC 1 cut(s) 324
BlsI GCNGC 1 cut(s) 118
Bme1390I CCNGG 1 cut(s) 70
BmiI GGNNCC 2 cut(s) 58, 465
BmrFI CCNGG 1 cut(s) 70
Bpu10I CCTNAGC 1 cut(s) 414
Bpu1102I GCTNAGC 1 cut(s) 324
BsaI GGTCTC 1 cut(s) 78
Bse118I RCCGGY 1 cut(s) 374
Bse1I ACTGG 1 cut(s) 303
BseBI CCWGG 1 cut(s) 70
BseGI GGATG 1 cut(s) 145
BseMII CTCAG 2 cut(s) 315, 405
BseNI ACTGG 1 cut(s) 303
BseRI GAGGAG 3 cut(s) 60, 355, 456
BseXI GCAGC 1 cut(s) 103
BseYI CCCAGC 1 cut(s) 120
BshFI GGCC 1 cut(s) 144
BsiSI CCGG 2 cut(s) 224, 375
BsmAI GTCTC 1 cut(s) 78
BsnI GGCC 1 cut(s) 144
Bso31I GGTCTC 1 cut(s) 78
Bsp143I GATC 1 cut(s) 10
Bsp1720I GCTNAGC 1 cut(s) 324
BspACI CCGC 1 cut(s) 489
BspANI GGCC 1 cut(s) 144
BspCNI CTCAG 2 cut(s) 316, 406
BspLI GGNNCC 2 cut(s) 58, 465
BspTNI GGTCTC 1 cut(s) 78
BsrFI RCCGGY 1 cut(s) 374
BsrI ACTGG 1 cut(s) 303
BssAI RCCGGY 1 cut(s) 374
BssMI GATC 1 cut(s) 10
Bst2UI CCWGG 1 cut(s) 70
Bst6I CTCTTC 1 cut(s) 453
BstC8I GCNNGC 1 cut(s) 368
BstDEI CTNAG 4 cut(s) 20, 30, 324, 414
BstF5I GGATG 1 cut(s) 145
BstKTI GATC 1 cut(s) 13
BstMAI GTCTC 1 cut(s) 78
BstMBI GATC 1 cut(s) 10
BstMWI GCNNNNNNNGC 2 cut(s) 91, 179
BstNI CCWGG 1 cut(s) 70
BstSCI CCNGG 1 cut(s) 68
BstV1I GCAGC 1 cut(s) 103
BsuRI GGCC 1 cut(s) 144
BtsCI GGATG 1 cut(s) 145
BtsIMutI CAGTG 1 cut(s) 296
Cac8I GCNNGC 1 cut(s) 368
Cfr10I RCCGGY 1 cut(s) 374
CviAII CATG 2 cut(s) 91, 329
DdeI CTNAG 4 cut(s) 20, 30, 324, 414
DpnI GATC 1 cut(s) 12
DpnII GATC 1 cut(s) 10
Eam1104I CTCTTC 1 cut(s) 453
EarI CTCTTC 1 cut(s) 453
Eco31I GGTCTC 1 cut(s) 78
EcoRII CCWGG 1 cut(s) 68
FaeI CATG 2 cut(s) 94, 332
FaiI YATR 7 cut(s) 42, 92, 184, 186, 245, 330, 432
FatI CATG 2 cut(s) 90, 328
FauNDI CATATG 1 cut(s) 184
Fnu4HI GCNGC 1 cut(s) 117
FokI GGATG 1 cut(s) 152
Fsp4HI GCNGC 1 cut(s) 117
FspBI CTAG 1 cut(s) 131
GluI GCNGC 1 cut(s) 117
GsaI CCCAGC 1 cut(s) 124
HaeIII GGCC 1 cut(s) 144
HapII CCGG 2 cut(s) 224, 375
Hin1II CATG 2 cut(s) 94, 332
HinfI GANTC 1 cut(s) 355
HpaII CCGG 2 cut(s) 224, 375
Hpy188I TCNGA 3 cut(s) 82, 113, 166
Hpy188III TCNNGA 2 cut(s) 131, 470
Hpy99I CGWCG 1 cut(s) 82
HpyAV CCTTC 4 cut(s) 28, 130, 200, 223
HpyCH4V TGCA 1 cut(s) 182
HpyF10VI GCNNNNNNNGC 2 cut(s) 91, 179
HpyF3I CTNAG 4 cut(s) 20, 30, 324, 414
Hsp92II CATG 2 cut(s) 94, 332
Kzo9I GATC 1 cut(s) 10
LmnI GCTCC 5 cut(s) 82, 99, 113, 401, 469
LpnPI CCDG 9 cut(s) 55, 78, 82, 134, 187, 237, 284, 388, 426
Lsp1109I GCAGC 1 cut(s) 103
MaeI CTAG 1 cut(s) 131
MalI GATC 1 cut(s) 12
MboI GATC 1 cut(s) 10
MboII GAAGA 4 cut(s) 113, 245, 470, 507
MfeI CAATTG 1 cut(s) 308
MluCI AATT 7 cut(s) 157, 213, 256, 308, 406, 451, 498
MlyI GAGTC 1 cut(s) 349
MmeI TCCRAC 2 cut(s) 60, 190
MnlI CCTC 4 cut(s) 38, 333, 454, 477
MseI TTAA 2 cut(s) 390, 483
MslI CAYNNNNRTG 1 cut(s) 435
MspI CCGG 2 cut(s) 224, 375
MspR9I CCNGG 1 cut(s) 70
MunI CAATTG 1 cut(s) 308
MvaI CCWGG 1 cut(s) 70
MwoI GCNNNNNNNGC 2 cut(s) 91, 179
NdeI CATATG 1 cut(s) 184
NdeII GATC 1 cut(s) 10
NlaIII CATG 2 cut(s) 94, 332
NlaIV GGNNCC 2 cut(s) 58, 465
PkrI GCNGC 1 cut(s) 118
PleI GAGTC 1 cut(s) 349
PpsI GAGTC 1 cut(s) 349
Psp6I CCWGG 1 cut(s) 68
PspFI CCCAGC 1 cut(s) 120
PspGI CCWGG 1 cut(s) 68
PspN4I GGNNCC 2 cut(s) 58, 465
RseI CAYNNNNRTG 1 cut(s) 435
SaqAI TTAA 2 cut(s) 390, 483
SatI GCNGC 1 cut(s) 117
Sau3AI GATC 1 cut(s) 10
SchI GAGTC 1 cut(s) 349
ScrFI CCNGG 1 cut(s) 70
SetI ASST 8 cut(s) 26, 39, 87, 118, 126, 199, 243, 406
SmiMI CAYNNNNRTG 1 cut(s) 435
Sse9I AATT 7 cut(s) 157, 213, 256, 308, 406, 451, 498
SsiI CCGC 1 cut(s) 489
SspMI CTAG 1 cut(s) 131
StyD4I CCNGG 1 cut(s) 68
TasI AATT 7 cut(s) 157, 213, 256, 308, 406, 451, 498
Tru1I TTAA 2 cut(s) 390, 483
Tru9I TTAA 2 cut(s) 390, 483
TscAI CASTG 1 cut(s) 303
TseI GCWGC 1 cut(s) 116
TspGWI ACGGA 2 cut(s) 66, 86
TspRI CASTG 1 cut(s) 303
XbaI TCTAGA 1 cut(s) 130
XspI CTAG 1 cut(s) 131
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.