RchiOBHm_Chr3g0448981

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
3
OG0010703
Physical Location & Seq
Reverse (-)
978438 .. 979770
1333 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 579 bp
ATGTTGGCGCCTGATCAAATGAGAATGAGTCTATTTTACAGAAATAAGCTCGGCCCTAATATCCACTTATACACTGACATCTGTATTTCGAGGCTGTACGAACTGGGAGCCCGTCAAGTCCTGGTCACAGGCACAGGACCACTAGGCTGTGTCCCCGCCGAGCTTGCCATGCATAGCCGAAACGGGGAGTGCTCCGTGGAGCTCCAACGCGCTGCCTCGCTCTTCAACCCGCAGCTAGTCCAAATGATCAGCTCGCTCAACAGCGATCTCGGGTCCAACGTCTTCATTGCCGCAAACGCCTACCAAATGCACATGGATTTCGTTAGTAACCCCGCTGCCTACGGGTTTGTAACATCAAAGATAGCCTGTTGTGGCCAAGGACCTTACAATGGGATCGGACTATGCACACCCCTTTCGAACCTGTGCCCGAACAGAGACTTGTACGCGTTTTGGGATCCGTTCCATCCTTCGGAGAAGGCTAATAGGATCATTGTGCAGCAAATCCTGACTGGGACCGAAGAGTACATGCACCCCATGAACCTCAGTACTATGTTGGCCTTGGACAACTCTAGGACCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0007275 GO:0008150 GO:0032501 GO:0032502 GO:0042335 GO:0048856
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

192

Amino Acids

21.21

Weight (kDa)

6.17

Isoelectric Point (pI)

46.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 21 - 167 2e-14 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010703)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G04290 AT5G33370 AT5G33370
fragaria_vesca FvH4_6g01390 FvH4_6g01390
malus_domestica MD04G1234300.v1.1 MD12G1252200.v1.1
prunus_persica Prupe.6G354100_v2.0.a1
pyrus_communis pycom04g20800
rosa_chinensis RchiOBHm_Chr3g0448981 RchiOBHm_Chr3g0449021
rosa_laevigata RLG00000025862
rosa_multiflora Rmu_sc0003116.1_g000011
rosa_roxburghii Rroxscaffold_6G00427140
rosa_rugosa Rorug02G0617400
rosa_samantha Rh3AG015900 Rh3BG016000 Rh3CG015000 Rh3DG016300
rosa_wichuraiana Rw3G001190

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 7
AccII CGCG 2 cut(s) 210, 446
AciI CCGC 4 cut(s) 156, 230, 291, 333
AclWI GGATC 4 cut(s) 401, 449, 462, 494
AcoI YGGCCR 1 cut(s) 373
AcyI GRCGYC 1 cut(s) 8
AfaI GTAC 4 cut(s) 98, 443, 524, 547
AfiI CCNNNNNNNGG 3 cut(s) 184, 389, 469
AflIII ACRYGT 1 cut(s) 444
AgsI TTSAA 1 cut(s) 226
AjnI CCWGG 1 cut(s) 120
AluBI AGCT 5 cut(s) 49, 163, 202, 235, 252
AluI AGCT 5 cut(s) 49, 163, 202, 235, 252
Alw21I GWGCWC 2 cut(s) 194, 204
Alw26I GTCTC 1 cut(s) 429
AlwI GGATC 4 cut(s) 401, 449, 462, 494
Ama87I CYCGRG 1 cut(s) 269
AoxI GGCC 3 cut(s) 52, 373, 555
ApeKI GCWGC 4 cut(s) 212, 232, 335, 496
AspLEI GCGC 2 cut(s) 10, 212
AspS9I GGNCC 6 cut(s) 53, 137, 273, 380, 513, 573
AsuII TTCGAA 1 cut(s) 416
AvaI CYCGRG 1 cut(s) 269
AvaII GGWCC 5 cut(s) 137, 273, 380, 513, 573
BaeGI GKGCMC 1 cut(s) 428
BalI TGGCCA 1 cut(s) 375
BamHI GGATCC 1 cut(s) 454
BanI GGYRCC 1 cut(s) 7
BanII GRGCYC 2 cut(s) 112, 204
BbsI GAAGAC 1 cut(s) 274
Bbv12I GWGCWC 2 cut(s) 194, 204
BbvI GCAGC 4 cut(s) 199, 244, 322, 508
BccI CCATC 1 cut(s) 471
BciT130I CCWGG 1 cut(s) 122
BclI TGATCA 2 cut(s) 13, 246
BcoDI GTCTC 1 cut(s) 429
BfaI CTAG 4 cut(s) 143, 236, 570, 577
BfoI RGCGCY 1 cut(s) 11
BisI GCNGC 5 cut(s) 213, 233, 291, 336, 497
BlsI GCNGC 5 cut(s) 214, 234, 292, 337, 498
BmcAI AGTACT 1 cut(s) 547
Bme1390I CCNGG 1 cut(s) 122
Bme18I GGWCC 5 cut(s) 137, 273, 380, 513, 573
BmeT110I CYCGRG 1 cut(s) 269
BmgT120I GGNCC 6 cut(s) 53, 137, 273, 380, 513, 573
BmiI GGNNCC 5 cut(s) 9, 109, 274, 456, 514
BmrFI CCNGG 1 cut(s) 122
BmrI ACTGGG 2 cut(s) 113, 519
BmuI ACTGGG 2 cut(s) 113, 519
BpiI GAAGAC 1 cut(s) 274
Bpu14I TTCGAA 1 cut(s) 416
BsaHI GRCGYC 1 cut(s) 8
BsaJI CCNNGG 3 cut(s) 195, 376, 558
Bsc4I CCNNNNNNNGG 3 cut(s) 184, 389, 469
Bse1I ACTGG 2 cut(s) 108, 514
Bse3DI GCAATG 1 cut(s) 285
BseBI CCWGG 1 cut(s) 122
BseDI CCNNGG 3 cut(s) 195, 376, 558
BseGI GGATG 1 cut(s) 463
BseLI CCNNNNNNNGG 3 cut(s) 184, 389, 469
BseMI GCAATG 1 cut(s) 285
BseMII CTCAG 1 cut(s) 556
BseNI ACTGG 2 cut(s) 108, 514
BseSI GKGCMC 1 cut(s) 428
BseXI GCAGC 4 cut(s) 199, 244, 322, 508
BsgI GTGCAG 1 cut(s) 515
Bsh1236I CGCG 2 cut(s) 210, 446
BshFI GGCC 3 cut(s) 54, 375, 557
BshNI GGYRCC 1 cut(s) 7
BsiHKAI GWGCWC 2 cut(s) 194, 204
BsiHKCI CYCGRG 1 cut(s) 269
BslFI GGGAC 2 cut(s) 137, 526
BslI CCNNNNNNNGG 3 cut(s) 184, 389, 469
BsmAI GTCTC 1 cut(s) 429
BsmFI GGGAC 2 cut(s) 137, 526
BsnI GGCC 3 cut(s) 54, 375, 557
BsoBI CYCGRG 1 cut(s) 269
Bsp119I TTCGAA 1 cut(s) 416
Bsp1286I GDGCHC 4 cut(s) 112, 194, 204, 428
Bsp143I GATC 6 cut(s) 13, 246, 265, 393, 454, 486
BspACI CCGC 4 cut(s) 156, 230, 291, 333
BspANI GGCC 3 cut(s) 54, 375, 557
BspCNI CTCAG 1 cut(s) 555
BspFNI CGCG 2 cut(s) 210, 446
BspLI GGNNCC 5 cut(s) 9, 109, 274, 456, 514
BspPI GGATC 4 cut(s) 401, 449, 462, 494
BspQI GCTCTTC 1 cut(s) 227
BspT104I TTCGAA 1 cut(s) 416
BspT107I GGYRCC 1 cut(s) 7
BsrDI GCAATG 1 cut(s) 285
BsrI ACTGG 2 cut(s) 108, 514
BssECI CCNNGG 3 cut(s) 195, 376, 558
BssMI GATC 6 cut(s) 13, 246, 265, 393, 454, 486
BssNI GRCGYC 1 cut(s) 8
BssT1I CCWWGG 2 cut(s) 376, 558
Bst2UI CCWGG 1 cut(s) 122
Bst6I CTCTTC 2 cut(s) 227, 513
BstACI GRCGYC 1 cut(s) 8
BstBI TTCGAA 1 cut(s) 416
BstC8I GCNNGC 2 cut(s) 165, 254
BstDEI CTNAG 1 cut(s) 542
BstDSI CCRYGG 1 cut(s) 195
BstF5I GGATG 1 cut(s) 463
BstFNI CGCG 2 cut(s) 210, 446
BstH2I RGCGCY 1 cut(s) 11
BstHHI GCGC 2 cut(s) 10, 212
BstKTI GATC 6 cut(s) 16, 249, 268, 396, 457, 489
BstMAI GTCTC 1 cut(s) 429
BstMBI GATC 6 cut(s) 13, 246, 265, 393, 454, 486
BstMWI GCNNNNNNNGC 3 cut(s) 164, 169, 296
BstNI CCWGG 1 cut(s) 122
BstNSI RCATGY 1 cut(s) 529
BstSCI CCNGG 1 cut(s) 120
BstSLI GKGCMC 1 cut(s) 428
BstUI CGCG 2 cut(s) 210, 446
BstV1I GCAGC 4 cut(s) 199, 244, 322, 508
BstV2I GAAGAC 1 cut(s) 274
BstX2I RGATCY 1 cut(s) 454
BstYI RGATCY 1 cut(s) 454
BsuRI GGCC 3 cut(s) 54, 375, 557
BtgI CCRYGG 1 cut(s) 195
BtsCI GGATG 1 cut(s) 463
BtsIMutI CAGTG 1 cut(s) 72
Cac8I GCNNGC 2 cut(s) 165, 254
CfoI GCGC 2 cut(s) 10, 212
Cfr13I GGNCC 6 cut(s) 53, 137, 273, 380, 513, 573
Csp6I GTAC 4 cut(s) 97, 442, 523, 546
CviAII CATG 4 cut(s) 169, 313, 526, 535
CviQI GTAC 4 cut(s) 97, 442, 523, 546
DdeI CTNAG 1 cut(s) 542
DinI GGCGCC 1 cut(s) 9
DpnI GATC 6 cut(s) 15, 248, 267, 395, 456, 488
DpnII GATC 6 cut(s) 13, 246, 265, 393, 454, 486
EaeI YGGCCR 1 cut(s) 373
Eam1104I CTCTTC 2 cut(s) 227, 513
EarI CTCTTC 2 cut(s) 227, 513
Ecl136II GAGCTC 1 cut(s) 202
Eco130I CCWWGG 2 cut(s) 376, 558
Eco24I GRGCYC 2 cut(s) 112, 204
Eco47I GGWCC 5 cut(s) 137, 273, 380, 513, 573
Eco53kI GAGCTC 1 cut(s) 202
Eco88I CYCGRG 1 cut(s) 269
EcoICRI GAGCTC 1 cut(s) 202
EcoO109I RGGNCCY 2 cut(s) 380, 573
EcoRII CCWGG 1 cut(s) 120
EcoT14I CCWWGG 2 cut(s) 376, 558
EcoT22I ATGCAT 1 cut(s) 174
EcoT38I GRGCYC 2 cut(s) 112, 204
EgeI GGCGCC 1 cut(s) 9
EheI GGCGCC 1 cut(s) 9
ErhI CCWWGG 2 cut(s) 376, 558
FaeI CATG 4 cut(s) 172, 316, 529, 538
FaiI YATR 8 cut(s) 70, 170, 174, 314, 403, 527, 536, 551
FaqI GGGAC 2 cut(s) 137, 526
FatI CATG 4 cut(s) 168, 312, 525, 534
FauI CCCGC 3 cut(s) 163, 237, 340
FbaI TGATCA 2 cut(s) 13, 246
Fnu4HI GCNGC 5 cut(s) 213, 233, 291, 336, 497
FokI GGATG 1 cut(s) 450
FriOI GRGCYC 2 cut(s) 112, 204
Fsp4HI GCNGC 5 cut(s) 213, 233, 291, 336, 497
FspBI CTAG 4 cut(s) 143, 236, 570, 577
GlaI GCGC 2 cut(s) 9, 211
GluI GCNGC 5 cut(s) 213, 233, 291, 336, 497
HaeII RGCGCY 1 cut(s) 11
HaeIII GGCC 3 cut(s) 54, 375, 557
HhaI GCGC 2 cut(s) 10, 212
Hin1I GRCGYC 1 cut(s) 8
Hin1II CATG 4 cut(s) 172, 316, 529, 538
Hin6I GCGC 2 cut(s) 8, 210
HinP1I GCGC 2 cut(s) 8, 210
HinfI GANTC 1 cut(s) 28
Hpy188I TCNGA 2 cut(s) 398, 472
Hpy188III TCNNGA 1 cut(s) 505
HpyAV CCTTC 2 cut(s) 469, 477
HpyCH4IV ACGT 1 cut(s) 279
HpyCH4V TGCA 5 cut(s) 172, 310, 405, 496, 529
HpyF10VI GCNNNNNNNGC 3 cut(s) 164, 169, 296
HpyF3I CTNAG 1 cut(s) 542
HpySE526I ACGT 1 cut(s) 279
Hsp92I GRCGYC 1 cut(s) 8
Hsp92II CATG 4 cut(s) 172, 316, 529, 538
HspAI GCGC 2 cut(s) 8, 210
KasI GGCGCC 1 cut(s) 7
Ksp22I TGATCA 2 cut(s) 13, 246
Kzo9I GATC 6 cut(s) 13, 246, 265, 393, 454, 486
LguI GCTCTTC 1 cut(s) 227
LmnI GCTCC 4 cut(s) 107, 197, 199, 207
Lsp1109I GCAGC 4 cut(s) 199, 244, 322, 508
MaeI CTAG 4 cut(s) 143, 236, 570, 577
MaeII ACGT 1 cut(s) 279
MaeIII GTNAC 3 cut(s) 124, 326, 349
MalI GATC 6 cut(s) 15, 248, 267, 395, 456, 488
MboI GATC 6 cut(s) 13, 246, 265, 393, 454, 486
MboII GAAGA 3 cut(s) 214, 274, 530
MflI RGATCY 1 cut(s) 454
MhlI GDGCHC 4 cut(s) 112, 194, 204, 428
MlsI TGGCCA 1 cut(s) 375
MluI ACGCGT 1 cut(s) 444
MluNI TGGCCA 1 cut(s) 375
Mly113I GGCGCC 1 cut(s) 8
MlyI GAGTC 1 cut(s) 37
MmeI TCCRAC 2 cut(s) 229, 300
MnlI CCTC 3 cut(s) 84, 226, 551
Mox20I TGGCCA 1 cut(s) 375
Mph1103I ATGCAT 1 cut(s) 174
MscI TGGCCA 1 cut(s) 375
Msp20I TGGCCA 1 cut(s) 375
MspA1I CMGCKG 1 cut(s) 335
MspR9I CCNGG 1 cut(s) 122
MvaI CCWGG 1 cut(s) 122
MvnI CGCG 2 cut(s) 210, 446
MwoI GCNNNNNNNGC 3 cut(s) 164, 169, 296
NarI GGCGCC 1 cut(s) 8
NdeII GATC 6 cut(s) 13, 246, 265, 393, 454, 486
NlaIII CATG 4 cut(s) 172, 316, 529, 538
NlaIV GGNNCC 5 cut(s) 9, 109, 274, 456, 514
NmeAIII GCCGAG 2 cut(s) 30, 184
NmuCI GTSAC 1 cut(s) 124
NsiI ATGCAT 1 cut(s) 174
NspI RCATGY 1 cut(s) 529
NspV TTCGAA 1 cut(s) 416
PciSI GCTCTTC 1 cut(s) 227
PcsI WCGNNNNNNNCGW 1 cut(s) 276
PkrI GCNGC 5 cut(s) 214, 234, 292, 337, 498
PleI GAGTC 1 cut(s) 36
PluTI GGCGCC 1 cut(s) 11
PpsI GAGTC 1 cut(s) 36
PpuMI RGGWCCY 2 cut(s) 380, 573
Psp124BI GAGCTC 1 cut(s) 204
Psp5II RGGWCCY 2 cut(s) 380, 573
Psp6I CCWGG 1 cut(s) 120
PspGI CCWGG 1 cut(s) 120
PspN4I GGNNCC 5 cut(s) 9, 109, 274, 456, 514
PspPI GGNCC 6 cut(s) 53, 137, 273, 380, 513, 573
PspPPI RGGWCCY 2 cut(s) 380, 573
PsuI RGATCY 1 cut(s) 454
RsaI GTAC 4 cut(s) 98, 443, 524, 547
RsaNI GTAC 4 cut(s) 97, 442, 523, 546
SacI GAGCTC 1 cut(s) 204
SapI GCTCTTC 1 cut(s) 227
SatI GCNGC 5 cut(s) 213, 233, 291, 336, 497
Sau3AI GATC 6 cut(s) 13, 246, 265, 393, 454, 486
Sau96I GGNCC 6 cut(s) 53, 137, 273, 380, 513, 573
ScaI AGTACT 1 cut(s) 547
SchI GAGTC 1 cut(s) 37
ScrFI CCNGG 1 cut(s) 122
SduI GDGCHC 4 cut(s) 112, 194, 204, 428
SfoI GGCGCC 1 cut(s) 9
SfuI TTCGAA 1 cut(s) 416
SinI GGWCC 5 cut(s) 137, 273, 380, 513, 573
SsiI CCGC 4 cut(s) 156, 230, 291, 333
SspDI GGCGCC 1 cut(s) 7
SspMI CTAG 4 cut(s) 143, 236, 570, 577
SstI GAGCTC 1 cut(s) 204
StyD4I CCNGG 1 cut(s) 120
StyI CCWWGG 2 cut(s) 376, 558
TaiI ACGT 1 cut(s) 282
TaqI TCGA 2 cut(s) 89, 416
TaqII GACCGA 1 cut(s) 530
TatI WGTACW 2 cut(s) 522, 545
TauI GCSGC 1 cut(s) 293
TscAI CASTG 1 cut(s) 79
TseFI GTSAC 1 cut(s) 124
TseI GCWGC 4 cut(s) 212, 232, 335, 496
Tsp45I GTSAC 1 cut(s) 124
TspDTI ATGAA 2 cut(s) 274, 551
TspGWI ACGGA 2 cut(s) 184, 447
TspRI CASTG 1 cut(s) 79
VpaK11BI GGWCC 5 cut(s) 137, 273, 380, 513, 573
XceI RCATGY 1 cut(s) 529
XspI CTAG 4 cut(s) 143, 236, 570, 577
ZrmI AGTACT 1 cut(s) 547
Zsp2I ATGCAT 1 cut(s) 174
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.