RchiOBHm_Chr3g0460091

acid phosphatase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
3
OG0006132
Physical Location & Seq
Reverse (-)
8337162 .. 8339555
2394 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 582 bp
ATGTGGTTCACAGTGGTTGTATTATCACTACTATGCAGGTGTGACCCTTACGATCCATCGGGGTTCCGAGCATGGGCTATGTCTGGAAAGTGTCCGGCAATTCCTGCCATGCTGAGATTGTTTAGCAACCTGGTGAATATGGGGTTTAAGGTTATTTTGCTTACTGGAAGGGATGAAGAAACACTTGGTCAAGTCACTATCGAAAACCTGAATAATCAAGGATTTGTTGATTATGAACGGTTGATTTTGAGGACCGAAGCTTACAAAGGGCAGGGCGCGGTGGCGTACAAGTCAGCGATTCGGAAGCAACTGGTGGAAGAAGGTTACAGGATATGGGGGAATGTAGGAGATCAATGGAGTGATCTTCACGGAGATTATATAGGCAACCGCACGTTTAAGCTTCCTAATCCCATGTACTTTGTTGCTGAAAAGATGCATGCATGGATCATGTCTGCTGTTGGAAGATATTCTACTAATGAGATTAAATTTTTCCATCTTCATGATCTCACACGAGACTTGGTTTTGTTAAAAGACAAAAGAATAAACTTTCTTCAAATTGTAAGTTTCTGCACATGTTGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Acid_phosphat_B

Protein Analysis

193

Amino Acids

22.29

Weight (kDa)

8.58

Isoelectric Point (pI)

31.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Acid_phosphat_B PF03767 12 - 141 1.1e-42 HAD superfamily, subfamily IIIB (Acid phosphatase)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 27
Acc36I ACCTGC 1 cut(s) 27
AccII CGCG 1 cut(s) 278
AciI CCGC 2 cut(s) 278, 388
AclWI GGATC 2 cut(s) 47, 452
AcsI RAATTY 1 cut(s) 485
AfaI GTAC 2 cut(s) 287, 416
AfiI CCNNNNNNNGG 1 cut(s) 73
AflIII ACRYGT 1 cut(s) 572
AgsI TTSAA 1 cut(s) 554
AjnI CCWGG 1 cut(s) 129
AjuI GAANNNNNNNTTGG 2 cut(s) 168, 200
AluBI AGCT 2 cut(s) 260, 400
AluI AGCT 2 cut(s) 260, 400
Alw26I GTCTC 1 cut(s) 507
AlwI GGATC 2 cut(s) 47, 452
ApoI RAATTY 1 cut(s) 485
Asp700I GAANNNNTTC 1 cut(s) 466
AspLEI GCGC 1 cut(s) 278
AspS9I GGNCC 1 cut(s) 252
AsuHPI GGTGA 1 cut(s) 145
AvaII GGWCC 1 cut(s) 252
BarI GAAGNNNNNNTAC 2 cut(s) 454, 486
BauI CACGAG 1 cut(s) 510
BccI CCATC 2 cut(s) 64, 501
BciT130I CCWGG 1 cut(s) 131
BcoDI GTCTC 1 cut(s) 507
BfuAI ACCTGC 1 cut(s) 27
Bme1390I CCNGG 1 cut(s) 131
Bme18I GGWCC 1 cut(s) 252
BmgT120I GGNCC 1 cut(s) 252
BmiI GGNNCC 1 cut(s) 65
BmrFI CCNGG 1 cut(s) 131
BmsI GCATC 1 cut(s) 423
Bsc4I CCNNNNNNNGG 1 cut(s) 73
Bse1I ACTGG 2 cut(s) 169, 315
BseBI CCWGG 1 cut(s) 131
BseGI GGATG 1 cut(s) 178
BseLI CCNNNNNNNGG 1 cut(s) 73
BseMII CTCAG 1 cut(s) 104
BseNI ACTGG 2 cut(s) 169, 315
BsgI GTGCAG 1 cut(s) 553
Bsh1236I CGCG 1 cut(s) 278
BsiSI CCGG 1 cut(s) 95
BslI CCNNNNNNNGG 1 cut(s) 73
BsmAI GTCTC 1 cut(s) 507
Bsp143I GATC 5 cut(s) 52, 349, 361, 444, 502
BspACI CCGC 2 cut(s) 278, 388
BspCNI CTCAG 1 cut(s) 105
BspFNI CGCG 1 cut(s) 278
BspHI TCATGA 1 cut(s) 499
BspLI GGNNCC 1 cut(s) 65
BspMI ACCTGC 1 cut(s) 27
BspPI GGATC 2 cut(s) 47, 452
BsrI ACTGG 2 cut(s) 169, 315
BssMI GATC 5 cut(s) 52, 349, 361, 444, 502
BssSI CACGAG 1 cut(s) 510
Bst2BI CACGAG 1 cut(s) 510
Bst2UI CCWGG 1 cut(s) 131
Bst4CI ACNGT 2 cut(s) 13, 240
BstAPI GCANNNNNTGC 1 cut(s) 104
BstC8I GCNNGC 1 cut(s) 438
BstDEI CTNAG 1 cut(s) 113
BstF5I GGATG 1 cut(s) 178
BstFNI CGCG 1 cut(s) 278
BstHHI GCGC 1 cut(s) 278
BstKTI GATC 5 cut(s) 55, 352, 364, 447, 505
BstMAI GTCTC 1 cut(s) 507
BstMBI GATC 5 cut(s) 52, 349, 361, 444, 502
BstMWI GCNNNNNNNGC 1 cut(s) 104
BstNI CCWGG 1 cut(s) 131
BstNSI RCATGY 2 cut(s) 440, 576
BstSCI CCNGG 1 cut(s) 129
BstUI CGCG 1 cut(s) 278
BtsCI GGATG 1 cut(s) 178
BtsIMutI CAGTG 1 cut(s) 18
BveI ACCTGC 1 cut(s) 27
Cac8I GCNNGC 1 cut(s) 438
CciI TCATGA 1 cut(s) 499
CfoI GCGC 1 cut(s) 278
Cfr13I GGNCC 1 cut(s) 252
CsiI ACCWGGT 1 cut(s) 129
Csp6I GTAC 2 cut(s) 286, 415
CviAII CATG 8 cut(s) 72, 109, 412, 437, 441, 448, 500, 573
CviJI RGCY 3 cut(s) 77, 260, 400
CviKI_1 RGCY 3 cut(s) 77, 260, 400
CviQI GTAC 2 cut(s) 286, 415
DdeI CTNAG 1 cut(s) 113
DpnI GATC 5 cut(s) 54, 351, 363, 446, 504
DpnII GATC 5 cut(s) 52, 349, 361, 444, 502
Eco47I GGWCC 1 cut(s) 252
EcoRII CCWGG 1 cut(s) 129
EcoT22I ATGCAT 2 cut(s) 438, 442
FaeI CATG 8 cut(s) 75, 112, 415, 440, 444, 451, 503, 576
FalI AAGNNNNNCTT 2 cut(s) 168, 200
FatI CATG 8 cut(s) 71, 108, 411, 436, 440, 447, 499, 572
FokI GGATG 1 cut(s) 185
GlaI GCGC 1 cut(s) 277
HapII CCGG 1 cut(s) 95
HhaI GCGC 1 cut(s) 278
Hin1II CATG 8 cut(s) 75, 112, 415, 440, 444, 451, 503, 576
Hin6I GCGC 1 cut(s) 276
HinP1I GCGC 1 cut(s) 276
HindIII AAGCTT 2 cut(s) 258, 398
HinfI GANTC 1 cut(s) 298
HpaII CCGG 1 cut(s) 95
HphI GGTGA 1 cut(s) 145
Hpy166II GTNNAC 1 cut(s) 9
Hpy188I TCNGA 2 cut(s) 68, 303
Hpy188III TCNNGA 2 cut(s) 84, 500
Hpy8I GTNNAC 1 cut(s) 9
HpyAV CCTTC 2 cut(s) 162, 314
HpyCH4III ACNGT 2 cut(s) 13, 240
HpyCH4IV ACGT 1 cut(s) 392
HpyCH4V TGCA 4 cut(s) 36, 436, 440, 570
HpyF10VI GCNNNNNNNGC 1 cut(s) 104
HpyF3I CTNAG 1 cut(s) 113
HpySE526I ACGT 1 cut(s) 392
Hsp92II CATG 8 cut(s) 75, 112, 415, 440, 444, 451, 503, 576
HspAI GCGC 1 cut(s) 276
Kzo9I GATC 5 cut(s) 52, 349, 361, 444, 502
LweI GCATC 1 cut(s) 423
MabI ACCWGGT 1 cut(s) 129
MaeII ACGT 1 cut(s) 392
MaeIII GTNAC 3 cut(s) 41, 193, 323
MalI GATC 5 cut(s) 54, 351, 363, 446, 504
MboI GATC 5 cut(s) 52, 349, 361, 444, 502
MboII GAAGA 6 cut(s) 188, 329, 356, 474, 488, 542
MluCI AATT 3 cut(s) 99, 485, 555
MmeI TCCRAC 1 cut(s) 439
MnlI CCTC 1 cut(s) 243
Mph1103I ATGCAT 2 cut(s) 438, 442
MroXI GAANNNNTTC 1 cut(s) 466
MseI TTAA 4 cut(s) 147, 396, 483, 527
MslI CAYNNNNRTG 3 cut(s) 31, 498, 577
MspI CCGG 1 cut(s) 95
MspR9I CCNGG 1 cut(s) 131
MvaI CCWGG 1 cut(s) 131
MvnI CGCG 1 cut(s) 278
MwoI GCNNNNNNNGC 1 cut(s) 104
NdeII GATC 5 cut(s) 52, 349, 361, 444, 502
NlaIII CATG 8 cut(s) 75, 112, 415, 440, 444, 451, 503, 576
NlaIV GGNNCC 1 cut(s) 65
NmuCI GTSAC 2 cut(s) 41, 193
NsiI ATGCAT 2 cut(s) 438, 442
NspI RCATGY 2 cut(s) 440, 576
PaeI GCATGC 1 cut(s) 440
PagI TCATGA 1 cut(s) 499
PaqCI CACCTGC 1 cut(s) 27
PciI ACATGT 1 cut(s) 572
PdmI GAANNNNTTC 1 cut(s) 466
PfeI GAWTC 1 cut(s) 298
PscI ACATGT 1 cut(s) 572
Psp6I CCWGG 1 cut(s) 129
PspGI CCWGG 1 cut(s) 129
PspN4I GGNNCC 1 cut(s) 65
PspPI GGNCC 1 cut(s) 252
RsaI GTAC 2 cut(s) 287, 416
RsaNI GTAC 2 cut(s) 286, 415
RseI CAYNNNNRTG 3 cut(s) 31, 498, 577
SaqAI TTAA 4 cut(s) 147, 396, 483, 527
Sau3AI GATC 5 cut(s) 52, 349, 361, 444, 502
Sau96I GGNCC 1 cut(s) 252
ScrFI CCNGG 1 cut(s) 131
SetI ASST 8 cut(s) 41, 132, 153, 210, 262, 325, 395, 402
SexAI ACCWGGT 1 cut(s) 129
SfaNI GCATC 1 cut(s) 423
SinI GGWCC 1 cut(s) 252
SmiMI CAYNNNNRTG 3 cut(s) 31, 498, 577
SphI GCATGC 1 cut(s) 440
Sse9I AATT 3 cut(s) 99, 485, 555
SsiI CCGC 2 cut(s) 278, 388
StyD4I CCNGG 1 cut(s) 129
TaaI ACNGT 2 cut(s) 13, 240
TaiI ACGT 1 cut(s) 395
TaqI TCGA 1 cut(s) 201
TaqII GACCGA 1 cut(s) 269
TasI AATT 3 cut(s) 99, 485, 555
TatI WGTACW 1 cut(s) 414
TfiI GAWTC 1 cut(s) 298
Tru1I TTAA 4 cut(s) 147, 396, 483, 527
Tru9I TTAA 4 cut(s) 147, 396, 483, 527
TscAI CASTG 1 cut(s) 18
TseFI GTSAC 2 cut(s) 41, 193
Tsp45I GTSAC 2 cut(s) 41, 193
TspDTI ATGAA 3 cut(s) 189, 249, 488
TspGWI ACGGA 1 cut(s) 384
TspRI CASTG 1 cut(s) 18
VpaK11BI GGWCC 1 cut(s) 252
XapI RAATTY 1 cut(s) 485
XceI RCATGY 2 cut(s) 440, 576
XmnI GAANNNNTTC 1 cut(s) 466
Zsp2I ATGCAT 2 cut(s) 438, 442
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.