Basic Information

Type: gene

Biological Identity

Species

rosa_chinensis

Chromosome

Orthogroup ID

OG0008099

Physical Location & Seq

Genomic Coordinates Reverse (-)

3690587 .. 3694157

Gene Structure 3571 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1335 bp

ATGCATTTAAGTGTACATGAAGACATTGTAGCCACTAATTTCAGAAATGAACATCAACCAATCAACTTGTGGCTAAGATGTTTCATAGCCAAAAAAGCAAGTTGCAATGCATGGGCACGCACCAAACCAACAGAGTCACACGCCCACCACAACCACCAAATGGCTAGCACACAACTTTCCGACCAAGTTTTCAGGCCAATCCCTGCCGTGTGCGTCAGTTTGCGGCAACTGAGCACCACCAAACCGAATAAACTACCATGCCACCCACGTCCCAAAGCCTTGAAACACAAACACTTGGGGATTCAAACAGCGCAGAACTACTCAAAGTGGGCTGTTAGACTTAGCTTAGTTGACCAAATCCCGCCAAAATCAACCGTCGACATGGAACGGTTAGTCGATTTTCTGTACCAGGATTTAGTGCACTTGTTTGATGATCAAGGAATTGATCGGACGGCGTATGATGAACGTGTGAAGTTCAGGGATCCAATCACCAAGCATGACTCTATAACTGGCTACTTGTTGAACATTGCCTTCTTGAAGACAGTCTTCACTCCCAAGTTCCAGTTGCATTGGGTCAAACAGTCAGGACCATATGAAATCACTACAAGGTGGACTATGGTTATGAAATTCATCCCTCTGCCTTGGAAACCAGAATTGATCTTCACTGGTACTTCTGTTATGGGAATCAACCCTGAAACCGGGAAGTTCTGCAGCCATGTGGACTTCTGGGATTCCATAAGGAAGAATGACTATTTTTCTGTAGAAGGTTTGTTAGATGTATTCAAGCAGCTGAGGATTTACAAAACTCCAGACTTGGAAACACCAAAATATGAGATACTAAAAAGAACAGCAAATTATGAGGTAAGAAAGTACTCCCCTTTTATAGTGGTAGAAGGAAGTGTAGACAAGCTGTCTGGATCAGCTGGTTTCAATGATGTTACTGGGTACATCTTCGGAAAGAACTCGAAAATGGAGAAGATGCCAATGACAACTCCAGTGTTCACAGAGGCATCTGATGCTGAATTTTCCAAAGTATCCATCAAAGTATGTCTTCCACTAGACAAGGATTTAAGCAGTTTACCAGATCCTAATGCAGAAACAATTAGCTTGAAAAAAATGGAAGAAGGCATTGCTGCTGTTACCAAGTTCAGTGGGAAGCCTACCGAAGATGTTGTTCGTGAAAAGGAGAAATTGCTGCGTGCTAGTCTTATTAGAGATGGTCTCAAACCTAAGGCCGGTTGTATGCTTGCTCGTTACAATGACCCCGGCCGAACTTGGAGCTTTATACTGAGAAATGAAGTGCTGATATGGCTGGAGGAATTCACATTGGAGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DUF2358 SOUL

Protein Analysis

444

Amino Acids

50.97

Weight (kDa)

8.99

Isoelectric Point (pI)

28.6

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DUF2358	PF10184	130 - 239	3.5e-28	Uncharacterized conserved protein (DUF2358)
SOUL	PF04832	272 - 436	3.7e-37	SOUL heme-binding protein

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008099)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G20140 AT5G20140
fragaria_vesca	FvH4_1g04030 FvH4_1g04030 FvH4_1g04030
malus_domestica	MD00G1026900.v1.1 MD15G1178700.v1.1
prunus_persica	Prupe.7G236600_v2.0.a1 Prupe.7G236600_v2.0.a1 Prupe.7G236600_v2.0.a1 Prupe.7G236600_v2.0.a1
pyrus_communis	pycom02g03170 pycom15g16060
rosa_chinensis	RchiOBHm_Chr2g0089501
rosa_laevigata	RLG00000016036
rosa_multiflora	Rmu_sc0006193.1_g000010
rosa_roxburghii	Rroxscaffold_2G00151870
rosa_rugosa	Rorug02G0001200 Rorug02G0001200 Rorug02G0001300
rosa_samantha	Rh2BG045200 Rh2CG047000 Rh2DG046600
rosa_wichuraiana	Rw2G003600

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB7I	CCANNNNNTGG	1 cut(s)	160
AccI	GTMKAC	2 cut(s)	378, 903
AciI	CCGC	2 cut(s)	223, 362
AclWI	GGATC	4 cut(s)	476, 489, 925, 1079
AcoI	YGGCCR	1 cut(s)	1267
AcsI	RAATTY	3 cut(s)	626, 1022, 1319
AfaI	GTAC	5 cut(s)	15, 407, 670, 872, 947
AfiI	CCNNNNNNNGG	3 cut(s)	160, 698, 1235
AflIII	ACRYGT	1 cut(s)	466
AgsI	TTSAA	7 cut(s)	283, 305, 523, 538, 784, 931, 1111
AhdI	GACNNNNNGTC	1 cut(s)	910
AjiI	CACGTC	1 cut(s)	269
AjnI	CCWGG	1 cut(s)	408
AluBI	AGCT	6 cut(s)	345, 790, 910, 923, 1107, 1281
AluI	AGCT	6 cut(s)	345, 790, 910, 923, 1107, 1281
Alw21I	GWGCWC	2 cut(s)	236, 423
Alw26I	GTCTC	1 cut(s)	1226
Alw44I	GTGCAC	1 cut(s)	419
AlwI	GGATC	4 cut(s)	476, 489, 925, 1079
AoxI	GGCC	3 cut(s)	194, 1233, 1267
ApaLI	GTGCAC	1 cut(s)	419
ApeKI	GCWGC	4 cut(s)	711, 787, 1133, 1195
ApoI	RAATTY	3 cut(s)	626, 1022, 1319
AspLEI	GCGC	1 cut(s)	313
AspS9I	GGNCC	1 cut(s)	587
AsuC2I	CCSGG	2 cut(s)	700, 1266
AsuHPI	GGTGA	1 cut(s)	481
AsuNHI	GCTAGC	1 cut(s)	164
AvaII	GGWCC	1 cut(s)	587
AxyI	CCTNAGG	1 cut(s)	1230
BaeGI	GKGCMC	2 cut(s)	118, 423
BaeI	ACNNNNGTAYC	2 cut(s)	660, 693
BamHI	GGATCC	1 cut(s)	481
BbsI	GAAGAC	4 cut(s)	27, 538, 545, 1043
Bbv12I	GWGCWC	2 cut(s)	236, 423
BbvCI	CCTCAGC	1 cut(s)	791
BbvI	GCAGC	4 cut(s)	723, 799, 1120, 1182
BccI	CCATC	2 cut(s)	1046, 1211
BceAI	ACGGC	2 cut(s)	191, 468
BciT130I	CCWGG	1 cut(s)	410
BciVI	GTATCC	1 cut(s)	1045
BclI	TGATCA	1 cut(s)	433
BcnI	CCSGG	2 cut(s)	700, 1266
BcoDI	GTCTC	1 cut(s)	1226
BfaI	CTAG	3 cut(s)	165, 1058, 1203
BfmI	CTRYAG	2 cut(s)	709, 759
BfuI	GTATCC	1 cut(s)	1045
BisI	GCNGC	5 cut(s)	224, 712, 788, 1134, 1196
BlsI	GCNGC	5 cut(s)	225, 713, 789, 1135, 1197
BmcAI	AGTACT	1 cut(s)	872
Bme1390I	CCNGG	3 cut(s)	410, 700, 1266
Bme18I	GGWCC	1 cut(s)	587
BmeRI	GACNNNNNGTC	1 cut(s)	910
BmgBI	CACGTC	1 cut(s)	269
BmgT120I	GGNCC	1 cut(s)	587
BmiI	GGNNCC	1 cut(s)	483
BmrFI	CCNGG	3 cut(s)	410, 700, 1266
BmrI	ACTGGG	1 cut(s)	951
BmsI	GCATC	3 cut(s)	969, 1006, 1019
BmtI	GCTAGC	1 cut(s)	168
BmuI	ACTGGG	1 cut(s)	951
BpiI	GAAGAC	4 cut(s)	27, 538, 545, 1043
BplI	GAGNNNNNCTC	2 cut(s)	1206, 1238
BpmI	CTGGAG	3 cut(s)	792, 978, 1334
Bpu10I	CCTNAGC	1 cut(s)	791
BpuMI	CCSGG	2 cut(s)	700, 1266
BsaI	GGTCTC	1 cut(s)	1226
BsaJI	CCNNGG	2 cut(s)	641, 1264
Bsc4I	CCNNNNNNNGG	3 cut(s)	160, 698, 1235
Bse118I	RCCGGY	1 cut(s)	1235
Bse1I	ACTGG	5 cut(s)	514, 562, 670, 946, 995
Bse21I	CCTNAGG	1 cut(s)	1230
Bse3DI	GCAATG	3 cut(s)	112, 525, 1128
BseBI	CCWGG	1 cut(s)	410
BseDI	CCNNGG	2 cut(s)	641, 1264
BseGI	GGATG	1 cut(s)	630
BseLI	CCNNNNNNNGG	3 cut(s)	160, 698, 1235
BseMI	GCAATG	3 cut(s)	112, 525, 1128
BseMII	CTCAG	3 cut(s)	221, 782, 1280
BseNI	ACTGG	5 cut(s)	514, 562, 670, 946, 995
BseSI	GKGCMC	2 cut(s)	118, 423
BseX3I	CGGCCG	1 cut(s)	1267
BseXI	GCAGC	4 cut(s)	723, 799, 1120, 1182
Bsh1285I	CGRYCG	1 cut(s)	1270
BshFI	GGCC	3 cut(s)	196, 1235, 1269
BsiEI	CGRYCG	1 cut(s)	1270
BsiHKAI	GWGCWC	2 cut(s)	236, 423
BsiSI	CCGG	3 cut(s)	699, 1236, 1266
BslFI	GGGAC	1 cut(s)	255
BslI	CCNNNNNNNGG	3 cut(s)	160, 698, 1235
BsmAI	GTCTC	1 cut(s)	1226
BsmFI	GGGAC	1 cut(s)	255
BsnI	GGCC	3 cut(s)	196, 1235, 1269
Bso31I	GGTCTC	1 cut(s)	1226
Bsp1286I	GDGCHC	3 cut(s)	118, 236, 423
Bsp1407I	TGTACA	1 cut(s)	13
Bsp143I	GATC	6 cut(s)	433, 445, 481, 657, 917, 1084
BspACI	CCGC	2 cut(s)	223, 362
BspANI	GGCC	3 cut(s)	196, 1235, 1269
BspCNI	CTCAG	3 cut(s)	222, 783, 1281
BspLI	GGNNCC	1 cut(s)	483
BspMAI	CTGCAG	1 cut(s)	713
BspOI	GCTAGC	1 cut(s)	168
BspPI	GGATC	4 cut(s)	476, 489, 925, 1079
BspTNI	GGTCTC	1 cut(s)	1226
BsrDI	GCAATG	3 cut(s)	112, 525, 1128
BsrFI	RCCGGY	1 cut(s)	1235
BsrGI	TGTACA	1 cut(s)	13
BsrI	ACTGG	5 cut(s)	514, 562, 670, 946, 995
BssAI	RCCGGY	1 cut(s)	1235
BssECI	CCNNGG	2 cut(s)	641, 1264
BssMI	GATC	6 cut(s)	433, 445, 481, 657, 917, 1084
BssT1I	CCWWGG	1 cut(s)	641
Bst2UI	CCWGG	1 cut(s)	410
Bst4CI	ACNGT	4 cut(s)	376, 390, 544, 582
BstAPI	GCANNNNNTGC	1 cut(s)	1016
BstAUI	TGTACA	1 cut(s)	13
BstC8I	GCNNGC	4 cut(s)	118, 166, 1200, 1248
BstDEI	CTNAG	7 cut(s)	74, 230, 341, 346, 791, 1230, 1289
BstF5I	GGATG	1 cut(s)	630
BstHHI	GCGC	1 cut(s)	313
BstKTI	GATC	6 cut(s)	436, 448, 484, 660, 920, 1087
BstMAI	GTCTC	1 cut(s)	1226
BstMBI	GATC	6 cut(s)	433, 445, 481, 657, 917, 1084
BstMCI	CGRYCG	1 cut(s)	1270
BstMWI	GCNNNNNNNGC	3 cut(s)	95, 1016, 1309
BstNI	CCWGG	1 cut(s)	410
BstSCI	CCNGG	3 cut(s)	408, 698, 1264
BstSFI	CTRYAG	2 cut(s)	709, 759
BstSLI	GKGCMC	2 cut(s)	118, 423
BstV1I	GCAGC	4 cut(s)	723, 799, 1120, 1182
BstV2I	GAAGAC	4 cut(s)	27, 538, 545, 1043
BstX2I	RGATCY	2 cut(s)	481, 1084
BstYI	RGATCY	2 cut(s)	481, 1084
BstZI	CGGCCG	1 cut(s)	1267
Bsu36I	CCTNAGG	1 cut(s)	1230
BsuI	GTATCC	1 cut(s)	1045
BsuRI	GGCC	3 cut(s)	196, 1235, 1269
BtrI	CACGTC	1 cut(s)	269
BtsCI	GGATG	1 cut(s)	630
BtsIMutI	CAGTG	3 cut(s)	663, 1002, 1156
Cac8I	GCNNGC	4 cut(s)	118, 166, 1200, 1248
CfoI	GCGC	1 cut(s)	313
Cfr10I	RCCGGY	1 cut(s)	1235
Cfr13I	GGNCC	1 cut(s)	587
CseI	GACGC	1 cut(s)	202
Csp6I	GTAC	5 cut(s)	14, 406, 669, 871, 946
CspCI	CAANNNNNGTGG	2 cut(s)	1132, 1167
CviAII	CATG	6 cut(s)	17, 111, 258, 382, 497, 716
CviQI	GTAC	5 cut(s)	14, 406, 669, 871, 946
DdeI	CTNAG	7 cut(s)	74, 230, 341, 346, 791, 1230, 1289
DpnI	GATC	6 cut(s)	435, 447, 483, 659, 919, 1086
DpnII	GATC	6 cut(s)	433, 445, 481, 657, 917, 1084
DriI	GACNNNNNGTC	1 cut(s)	910
EaeI	YGGCCR	1 cut(s)	1267
EagI	CGGCCG	1 cut(s)	1267
Eam1105I	GACNNNNNGTC	1 cut(s)	910
EclXI	CGGCCG	1 cut(s)	1267
Eco130I	CCWWGG	1 cut(s)	641
Eco31I	GGTCTC	1 cut(s)	1226
Eco47I	GGWCC	1 cut(s)	587
Eco52I	CGGCCG	1 cut(s)	1267
Eco81I	CCTNAGG	1 cut(s)	1230
EcoRI	GAATTC	1 cut(s)	1319
EcoRII	CCWGG	1 cut(s)	408
EcoT14I	CCWWGG	1 cut(s)	641
EcoT22I	ATGCAT	2 cut(s)	6, 112
ErhI	CCWWGG	1 cut(s)	641
FaeI	CATG	6 cut(s)	20, 114, 261, 385, 500, 719
FalI	AAGNNNNNCTT	4 cut(s)	530, 562, 1035, 1067
FaqI	GGGAC	1 cut(s)	255
FatI	CATG	6 cut(s)	16, 110, 257, 381, 496, 715
FauI	CCCGC	1 cut(s)	369
FauNDI	CATATG	1 cut(s)	592
FbaI	TGATCA	1 cut(s)	433
FblI	GTMKAC	2 cut(s)	378, 903
Fnu4HI	GCNGC	5 cut(s)	224, 712, 788, 1134, 1196
FokI	GGATG	1 cut(s)	617
Fsp4HI	GCNGC	5 cut(s)	224, 712, 788, 1134, 1196
FspBI	CTAG	3 cut(s)	165, 1058, 1203
GlaI	GCGC	1 cut(s)	312
GluI	GCNGC	5 cut(s)	224, 712, 788, 1134, 1196
GsuI	CTGGAG	3 cut(s)	792, 978, 1334
HaeIII	GGCC	3 cut(s)	196, 1235, 1269
HapII	CCGG	3 cut(s)	699, 1236, 1266
HgaI	GACGC	1 cut(s)	202
HhaI	GCGC	1 cut(s)	313
Hin1II	CATG	6 cut(s)	20, 114, 261, 385, 500, 719
Hin6I	GCGC	1 cut(s)	311
HinP1I	GCGC	1 cut(s)	311
HincII	GTYRAC	2 cut(s)	352, 379
HindII	GTYRAC	2 cut(s)	352, 379
HinfI	GANTC	5 cut(s)	134, 301, 500, 684, 731
HpaII	CCGG	3 cut(s)	699, 1236, 1266
HphI	GGTGA	1 cut(s)	481
Hpy166II	GTNNAC	9 cut(s)	14, 352, 379, 421, 612, 721, 904, 1002, 1079
Hpy188I	TCNGA	5 cut(s)	44, 181, 450, 956, 1015
Hpy188III	TCNNGA	5 cut(s)	535, 585, 809, 915, 1178
Hpy8I	GTNNAC	9 cut(s)	14, 352, 379, 421, 612, 721, 904, 1002, 1079
Hpy99I	CGWCG	1 cut(s)	380
HpyAV	CCTTC	4 cut(s)	541, 758, 887, 1118
HpyCH4III	ACNGT	4 cut(s)	376, 390, 544, 582
HpyCH4IV	ACGT	2 cut(s)	268, 466
HpyCH4V	TGCA	7 cut(s)	4, 105, 110, 421, 568, 711, 1094
HpyF10VI	GCNNNNNNNGC	3 cut(s)	95, 1016, 1309
HpyF3I	CTNAG	7 cut(s)	74, 230, 341, 346, 791, 1230, 1289
HpySE526I	ACGT	2 cut(s)	268, 466
Hsp92II	CATG	6 cut(s)	20, 114, 261, 385, 500, 719
HspAI	GCGC	1 cut(s)	311
Ksp22I	TGATCA	1 cut(s)	433
Kzo9I	GATC	6 cut(s)	433, 445, 481, 657, 917, 1084
LmnI	GCTCC	1 cut(s)	1278
Lsp1109I	GCAGC	4 cut(s)	723, 799, 1120, 1182
LweI	GCATC	3 cut(s)	969, 1006, 1019
MaeI	CTAG	3 cut(s)	165, 1058, 1203
MaeII	ACGT	2 cut(s)	268, 466
MaeIII	GTNAC	4 cut(s)	135, 937, 1138, 1253
MalI	GATC	6 cut(s)	435, 447, 483, 659, 919, 1086
MboI	GATC	6 cut(s)	433, 445, 481, 657, 917, 1084
MflI	RGATCY	2 cut(s)	481, 1084
MhlI	GDGCHC	3 cut(s)	118, 236, 423
MluCI	AATT	9 cut(s)	37, 441, 626, 653, 853, 1022, 1101, 1190, 1319
MlyI	GAGTC	2 cut(s)	143, 494
MmeI	TCCRAC	1 cut(s)	204
MnlI	CCTC	5 cut(s)	645, 786, 853, 1000, 1309
Mph1103I	ATGCAT	2 cut(s)	6, 112
MseI	TTAA	2 cut(s)	8, 1070
MslI	CAYNNNNRTG	1 cut(s)	9
MspA1I	CMGCKG	2 cut(s)	790, 923
MspI	CCGG	3 cut(s)	699, 1236, 1266
MspR9I	CCNGG	3 cut(s)	410, 700, 1266
MvaI	CCWGG	1 cut(s)	410
MwoI	GCNNNNNNNGC	3 cut(s)	95, 1016, 1309
NciI	CCSGG	2 cut(s)	700, 1266
NdeI	CATATG	1 cut(s)	592
NdeII	GATC	6 cut(s)	433, 445, 481, 657, 917, 1084
NheI	GCTAGC	1 cut(s)	164
NlaIII	CATG	6 cut(s)	20, 114, 261, 385, 500, 719
NlaIV	GGNNCC	1 cut(s)	483
NmuCI	GTSAC	1 cut(s)	135
NsiI	ATGCAT	2 cut(s)	6, 112
PfeI	GAWTC	3 cut(s)	301, 684, 731
PflMI	CCANNNNNTGG	1 cut(s)	160
PkrI	GCNGC	5 cut(s)	225, 713, 789, 1135, 1197
PleI	GAGTC	2 cut(s)	142, 494
PpsI	GAGTC	2 cut(s)	142, 494
Psp6I	CCWGG	1 cut(s)	408
PspGI	CCWGG	1 cut(s)	408
PspN4I	GGNNCC	1 cut(s)	483
PspPI	GGNCC	1 cut(s)	587
PstI	CTGCAG	1 cut(s)	713
PsuI	RGATCY	2 cut(s)	481, 1084
PvuII	CAGCTG	2 cut(s)	790, 923
RsaI	GTAC	5 cut(s)	15, 407, 670, 872, 947
RsaNI	GTAC	5 cut(s)	14, 406, 669, 871, 946
RseI	CAYNNNNRTG	1 cut(s)	9
SalI	GTCGAC	1 cut(s)	377
SaqAI	TTAA	2 cut(s)	8, 1070
SatI	GCNGC	5 cut(s)	224, 712, 788, 1134, 1196
Sau3AI	GATC	6 cut(s)	433, 445, 481, 657, 917, 1084
Sau96I	GGNCC	1 cut(s)	587
ScaI	AGTACT	1 cut(s)	872
SchI	GAGTC	2 cut(s)	143, 494
ScrFI	CCNGG	3 cut(s)	410, 700, 1266
SduI	GDGCHC	3 cut(s)	118, 236, 423
SfaNI	GCATC	3 cut(s)	969, 1006, 1019
SfcI	CTRYAG	2 cut(s)	709, 759
SinI	GGWCC	1 cut(s)	587
SmiMI	CAYNNNNRTG	1 cut(s)	9
Sse9I	AATT	9 cut(s)	37, 441, 626, 653, 853, 1022, 1101, 1190, 1319
SsiI	CCGC	2 cut(s)	223, 362
SspMI	CTAG	3 cut(s)	165, 1058, 1203
StyD4I	CCNGG	3 cut(s)	408, 698, 1264
StyI	CCWWGG	1 cut(s)	641
TaaI	ACNGT	4 cut(s)	376, 390, 544, 582
TaiI	ACGT	2 cut(s)	271, 469
TaqI	TCGA	3 cut(s)	378, 396, 965
TasI	AATT	9 cut(s)	37, 441, 626, 653, 853, 1022, 1101, 1190, 1319
TatI	WGTACW	2 cut(s)	13, 870
TauI	GCSGC	1 cut(s)	226
TfiI	GAWTC	3 cut(s)	301, 684, 731
Tru1I	TTAA	2 cut(s)	8, 1070
Tru9I	TTAA	2 cut(s)	8, 1070
TscAI	CASTG	3 cut(s)	670, 1002, 1156
TseFI	GTSAC	1 cut(s)	135
TseI	GCWGC	4 cut(s)	711, 787, 1133, 1195
Tsp45I	GTSAC	1 cut(s)	135
TspDTI	ATGAA	8 cut(s)	33, 63, 73, 477, 609, 619, 638, 1311
TspRI	CASTG	3 cut(s)	670, 1002, 1156
Van91I	CCANNNNNTGG	1 cut(s)	160
VneI	GTGCAC	1 cut(s)	419
VpaK11BI	GGWCC	1 cut(s)	587
XapI	RAATTY	3 cut(s)	626, 1022, 1319
XcmI	CCANNNNNNNNNTGG	1 cut(s)	66
XmiI	GTMKAC	2 cut(s)	378, 903
XspI	CTAG	3 cut(s)	165, 1058, 1203
ZrmI	AGTACT	1 cut(s)	872
Zsp2I	ATGCAT	2 cut(s)	6, 112

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.