RchiOBHm_Chr2g0155681

Protein of unknown function (DUF1296)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0007082
Physical Location & Seq
Reverse (-)
72542981 .. 72551335
8355 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 2625 bp
ATGAGTAGTGGTGGTGGGTTTAGGGTTTCAATCCCAAACAGTGTGAGGAAGACGATCCAGGACATCAAAGAGATCACAGGCAATCACAGCGAGGAAGAAATCTATGCAATGCTCAAGGAATGTAACATGGATCCCAACGAGACCGCGCAGAAACTTCTCCTTCAGGATCCATTTCATGAGGTCAAAAGGAAACGTGACAAGAGAAAAGAGAATCTGAACAACAGAGAGTCTGCAGAGCCACGGTGGAGGCCTGGAATGCCGGGTAGGGGGGGTAGGGGTGGTCGGGTGAATTTTTCATCTCGTCCACCACATGATGCCGGTGGTGGCAGGAACTCAGGTCCTGGAACTGAGAATGGACCCGCTCAGGTTGCAGAGAAAGGTGTTGCTCCCTCTTTGCCAACCACTCGTGAGACAAAAACTAAAGAGAGAAGTCTGGTAACAAGCTCTGTAACTGTGATTGTGGGTGGTCCTTCCAATGTGGCTTCGGGAACTACTACTGTTGTGCCTGCCTCACAGTTATCTACTGGAACTAGTGCTGAAATAAGTTTGTCATTGGTTGGGGATAAATCGGGTAGCTCGGTCTCTGCAGTGGATGCAAAAAAAATCCCCACCAGTGCATTCGGAAATGAGGACTTGCACGAGCAGGCTGCACCAAGCTCCAGTAGTAGCTCAGTTTTGCCAAACCCAGCGTCTACATTGGGAGCATGCTTTTCCCCCTCTGATCCTGTACTGGTGCCGTCTAATGATTCACGGCTCCCAGGTTCTGTGGGCACAATCAAACGTGAAGTTGTGAGCCATCATCCTCCTGCTTCTGAGGTTGGCAGCTCTTTAGCACAAGGAAAAACAACAAGCAAAACACAGGGAGTTGGGAAAACTCAGCCCTCTGATTTTTCACATCCTTCATCTACATCAACTCATGGCGGTTCGGTTAGTCGGCCATCATCTAATTATAGTAGCCGGTCGCAACAACTAATTGGCACTCAAAAAGTTGGTACCAACAAGGAGTGGAAACCAAAGCCCATAGTTTCTGCTGTTGTTCAAGGTCAAGCAACAGCTAATGTAGCTGCACCTGAGGTGCCTGCTGATTTGGTAGAGGTCTCTCCGCAGTCACAACCTGTTTCAAGTGTCCTTGATTCGGAAGAAGCAACTTCAAAACTGCAGAAGAAGCTAGAGGAATTACACCTTCCACAGCTTCCACAGCGCAAATTAGTTATTCTTCCAAATCACATACACGTTCCTGAGTCTGAACGAAACAAGTTGAGTTTTGGAAGCTTTGGGGCAACTTTTGGTGTAACGAATAGCTATGTCAGTGGTCCTGAGAGTGAGAAGAGCTCTACCCCTCGGTCTGAAACTTCTCAGGTTATTGAAGAATCTGTGGAGGAACAGTCCTCAAGCAATCAAAATGTGTTGGCAACTGCTGATGAGGGTGATTTTCCTGATCATTCGCAATCACCTACTCATATGCTTGAAAATCTATCATCTGTTGAGGGTGATGTCTCATCCAGTGCAGCCCAAGGACATAATGAATCTAAGCATGACACTGTATTGACTTCTGGAGGTCATCAGCTCCCAGTGGCTAATACTTCTCCGAATTATAGTTTTGGTATTGTACCTCCAATTTTAGGGAGTCAGCTTGCGGCATTTGAAAACTCTGAATCTCAAGCACGTGATATATCTCGGCTTCCAAGCTTTGTAGTACAACAACCCCTTGACCCAGCAAGTTATTATGCCCAATTTTACCGCTCAGGTGCTGATAGTGATGGCCGTCTTTCTCCTTTTCCATCTCCTGGGGTATCAACAAAGTACAATGGCAATGTTGGAGTATTGCCTCCAAGTTCTCAGTCTCCACAAGAGGGTGGGGTTCTTTCAGCAGCAGGTCCAACACCATTGGTGACACAGGCTTCTGGGCTTGTGCAAAGCTCAATAGCTGTAACTCAGCAGCCAGTTCCTGTCTTCCGACCGCCAGCTGGGGTGCACATTCCTCATTATGCTAACTACATTCCTTACAGTCACTACTTTAACCCATTCTATGTTCCGCCTCCTATCCACCAATATTTGGGTAATGGAGCATTTCCTCAACAACCTCAAGCAGGTGGTGTGTATCCAGCTCCACCAGCAGCTGCTGCCACAGGAGTCAAATACTCACTGCCACAATACAAAGCAGGGACTAATACAGGAAACTCAAATCACATGGGAATGGCAAGTGGATATGGGCCCTATGGCTCTTCCCCAGCTGGTTATAATCCCAGTCCTGCCACGACTGCAGGAAACACTACTCCCAATGAAGATCTTAGCACATCTCAGTTCAAGGAGAGTAATGTATACATCACCGGACAGCAGAGTGAAGGTTCATCTGTATGGGTTGCTGCACCTAACCGAGAAATGCCCAGCTTAACAAGTTCATTTTACAACCTTCCTCCACAGGGTCAGCATGTGACTTTCACTCCAACGCAGGCTGGCCATGGTTTCTACCACCCTGCACAAGCAGTATCAGCTGCAGCTGTCCATCCACTTCTGCAACAGTCCCAAACCATGGCTGGGGCAGTTGACATGGTGGGACCTGGGGGAAGTGTTTATCAGCAGCCTCAGCATGCACAACAAATCAACTGGCCGAGCAACTACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1296

Protein Analysis

874

Amino Acids

91.85

Weight (kDa)

6.3

Isoelectric Point (pI)

62.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GIP1_N PF06972 11 - 70 1e-34 GBF-interacting protein 1 N-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2241
AarI CACCTGC 1 cut(s) 2081
Acc36I ACCTGC 2 cut(s) 1865, 2081
Acc65I GGTACC 1 cut(s) 992
AccB1I GGYRCC 3 cut(s) 733, 992, 1075
AccB7I CCANNNNNTGG 3 cut(s) 1787, 2056, 2533
AccBSI CCGCTC 2 cut(s) 362, 1743
AccI GTMKAC 2 cut(s) 692, 2322
AccII CGCG 1 cut(s) 146
AciI CCGC 8 cut(s) 144, 360, 921, 1103, 1637, 1741, 1961, 2036
AclWI GGATC 6 cut(s) 49, 125, 138, 161, 174, 716
AcoI YGGCCR 4 cut(s) 935, 1762, 2458, 2609
AcsI RAATTY 1 cut(s) 289
AcuI CTGAAG 1 cut(s) 146
AcvI CACGTG 1 cut(s) 1667
AfaI GTAC 5 cut(s) 729, 994, 1611, 1698, 1805
AflIII ACRYGT 1 cut(s) 1231
AgsI TTSAA 8 cut(s) 30, 1040, 1122, 1152, 1367, 1469, 1646, 2308
AhlI ACTAGT 1 cut(s) 530
AjnI CCWGG 6 cut(s) 57, 250, 340, 757, 1786, 2560
Alw21I GWGCWC 2 cut(s) 1334, 1977
Alw26I GTCTC 6 cut(s) 134, 404, 586, 1102, 1501, 1848
Alw44I GTGCAC 1 cut(s) 1973
AlwI GGATC 6 cut(s) 49, 125, 138, 161, 174, 716
AlwNI CAGNNNCTG 6 cut(s) 341, 764, 1751, 1949, 2120, 2123
AoxI GGCC 6 cut(s) 248, 935, 1762, 2213, 2458, 2609
ApaI GGGCCC 1 cut(s) 2217
ApaLI GTGCAC 1 cut(s) 1973
ApoI RAATTY 1 cut(s) 289
ArsI GACNNNNNNTTYG 2 cut(s) 674, 706
Asp718I GGTACC 1 cut(s) 992
AspLEI GCGC 2 cut(s) 148, 1203
AspS9I GGNCC 8 cut(s) 338, 356, 467, 1313, 1877, 2213, 2214, 2558
AsuC2I CCSGG 1 cut(s) 261
AsuHPI GGTGA 6 cut(s) 298, 1439, 1443, 1502, 1903, 2320
AvaII GGWCC 6 cut(s) 338, 356, 467, 1313, 1877, 2558
AxyI CCTNAGG 1 cut(s) 1071
BaeGI GKGCMC 3 cut(s) 773, 1977, 2217
BalI TGGCCA 1 cut(s) 2460
BamHI GGATCC 2 cut(s) 130, 166
BanI GGYRCC 3 cut(s) 733, 992, 1075
BanII GRGCYC 2 cut(s) 1334, 2217
BauI CACGAG 2 cut(s) 405, 638
BbrPI CACGTG 1 cut(s) 1667
BbsI GAAGAC 2 cut(s) 56, 1945
Bbv12I GWGCWC 2 cut(s) 1334, 1977
BbvCI CCTCAGC 1 cut(s) 2586
BccI CCATC 5 cut(s) 804, 946, 1754, 1789, 2514
BceAI ACGGC 3 cut(s) 721, 767, 1749
BcgI CGANNNNNNTGC 2 cut(s) 629, 663
BciT130I CCWGG 6 cut(s) 59, 252, 342, 759, 1788, 2562
BciVI GTATCC 1 cut(s) 2112
BclI TGATCA 1 cut(s) 1438
BcnI CCSGG 1 cut(s) 261
BcoDI GTCTC 6 cut(s) 134, 404, 586, 1102, 1501, 1848
BcuI ACTAGT 1 cut(s) 530
BfaI CTAG 2 cut(s) 531, 1169
BfmI CTRYAG 5 cut(s) 231, 585, 1157, 2262, 2496
BfuAI ACCTGC 2 cut(s) 1865, 2081
BfuI GTATCC 1 cut(s) 2112
BglII AGATCT 1 cut(s) 2287
Bme1390I CCNGG 7 cut(s) 59, 252, 261, 342, 759, 1788, 2562
Bme18I GGWCC 6 cut(s) 338, 356, 467, 1313, 1877, 2558
BmgT120I GGNCC 8 cut(s) 338, 356, 467, 1313, 1877, 2213, 2214, 2558
BmiI GGNNCC 9 cut(s) 132, 168, 358, 735, 755, 994, 1077, 2215, 2559
BmrFI CCNGG 7 cut(s) 59, 252, 261, 342, 759, 1788, 2562
BmrI ACTGGG 2 cut(s) 1565, 2241
BmsI GCATC 2 cut(s) 304, 583
BmuI ACTGGG 2 cut(s) 1565, 2241
BpiI GAAGAC 2 cut(s) 56, 1945
BplI GAGNNNNNCTC 2 cut(s) 1316, 1348
BpmI CTGGAG 2 cut(s) 643, 1575
Bpu10I CCTNAGC 3 cut(s) 363, 1744, 2586
BpuEI CTTGAG 4 cut(s) 98, 1375, 1644, 2070
BpuMI CCSGG 1 cut(s) 261
BsaAI YACGTR 1 cut(s) 1667
BsaI GGTCTC 3 cut(s) 134, 586, 1102
BsaJI CCNNGG 8 cut(s) 239, 757, 1340, 1513, 1787, 2461, 2532, 2561
BsaWI WCCGGW 1 cut(s) 2330
BsaXI ACNNNNNCTCC 4 cut(s) 1370, 1400, 2428, 2458
Bse118I RCCGGY 2 cut(s) 317, 957
Bse1I ACTGG 9 cut(s) 529, 612, 660, 735, 1503, 1571, 1943, 2247, 2612
Bse21I CCTNAGG 1 cut(s) 1071
Bse3DI GCAATG 2 cut(s) 114, 1819
BseBI CCWGG 6 cut(s) 59, 252, 342, 759, 1788, 2562
BseDI CCNNGG 8 cut(s) 239, 757, 1340, 1513, 1787, 2461, 2532, 2561
BseGI GGATG 5 cut(s) 598, 799, 895, 1499, 2506
BseMI GCAATG 2 cut(s) 114, 1819
BseNI ACTGG 9 cut(s) 529, 612, 660, 735, 1503, 1571, 1943, 2247, 2612
BseSI GKGCMC 3 cut(s) 773, 1977, 2217
BseYI CCCAGC 6 cut(s) 685, 1714, 1967, 2230, 2387, 2537
BsgI GTGCAG 5 cut(s) 633, 1050, 1527, 2352, 2463
Bsh1236I CGCG 1 cut(s) 146
Bsh1285I CGRYCG 2 cut(s) 962, 1961
BshFI GGCC 6 cut(s) 250, 937, 1764, 2215, 2460, 2611
BshNI GGYRCC 3 cut(s) 733, 992, 1075
BsiEI CGRYCG 2 cut(s) 962, 1961
BsiHKAI GWGCWC 2 cut(s) 1334, 1977
BsiSI CCGG 4 cut(s) 260, 318, 958, 2331
BslFI GGGAC 3 cut(s) 2179, 2509, 2571
BsmAI GTCTC 6 cut(s) 134, 404, 586, 1102, 1501, 1848
BsmFI GGGAC 3 cut(s) 2179, 2509, 2571
BsmI GAATGC 2 cut(s) 261, 617
BsnI GGCC 6 cut(s) 250, 937, 1764, 2215, 2460, 2611
Bso31I GGTCTC 3 cut(s) 134, 586, 1102
Bsp120I GGGCCC 1 cut(s) 2213
Bsp1286I GDGCHC 4 cut(s) 773, 1334, 1977, 2217
Bsp143I GATC 7 cut(s) 54, 72, 130, 166, 721, 1438, 2287
Bsp19I CCATGG 2 cut(s) 2461, 2532
BspACI CCGC 8 cut(s) 144, 360, 921, 1103, 1637, 1741, 1961, 2036
BspANI GGCC 6 cut(s) 250, 937, 1764, 2215, 2460, 2611
BspFNI CGCG 1 cut(s) 146
BspHI TCATGA 1 cut(s) 175
BspLI GGNNCC 9 cut(s) 132, 168, 358, 735, 755, 994, 1077, 2215, 2559
BspMAI CTGCAG 5 cut(s) 235, 589, 1161, 2266, 2500
BspMI ACCTGC 2 cut(s) 1865, 2081
BspPI GGATC 6 cut(s) 49, 125, 138, 161, 174, 716
BspQI GCTCTTC 2 cut(s) 1322, 2230
BspT107I GGYRCC 3 cut(s) 733, 992, 1075
BspTNI GGTCTC 3 cut(s) 134, 586, 1102
BsrBI CCGCTC 2 cut(s) 362, 1743
BsrDI GCAATG 2 cut(s) 114, 1819
BsrFI RCCGGY 2 cut(s) 317, 957
BsrI ACTGG 9 cut(s) 529, 612, 660, 735, 1503, 1571, 1943, 2247, 2612
BssAI RCCGGY 2 cut(s) 317, 957
BssECI CCNNGG 8 cut(s) 239, 757, 1340, 1513, 1787, 2461, 2532, 2561
BssMI GATC 7 cut(s) 54, 72, 130, 166, 721, 1438, 2287
BssNAI GTATAC 1 cut(s) 2323
BssSI CACGAG 2 cut(s) 405, 638
BssT1I CCWWGG 3 cut(s) 1513, 2461, 2532
Bst1107I GTATAC 1 cut(s) 2323
Bst2BI CACGAG 2 cut(s) 405, 638
Bst2UI CCWGG 6 cut(s) 59, 252, 342, 759, 1788, 2562
Bst4CI ACNGT 9 cut(s) 41, 243, 454, 499, 516, 1386, 1543, 2009, 2523
Bst6I CTCTTC 2 cut(s) 1322, 2230
BstAPI GCANNNNNTGC 2 cut(s) 593, 2123
BstBAI YACGTR 1 cut(s) 1667
BstC8I GCNNGC 9 cut(s) 507, 645, 706, 1080, 1635, 1965, 2454, 2458, 2592
BstDSI CCRYGG 3 cut(s) 239, 2461, 2532
BstENI CCTNNNNNAGG 1 cut(s) 2088
BstF5I GGATG 5 cut(s) 598, 799, 895, 1499, 2506
BstFNI CGCG 1 cut(s) 146
BstHHI GCGC 2 cut(s) 148, 1203
BstKTI GATC 7 cut(s) 57, 75, 133, 169, 724, 1441, 2290
BstMAI GTCTC 6 cut(s) 134, 404, 586, 1102, 1501, 1848
BstMBI GATC 7 cut(s) 54, 72, 130, 166, 721, 1438, 2287
BstMCI CGRYCG 2 cut(s) 962, 1961
BstNI CCWGG 6 cut(s) 59, 252, 342, 759, 1788, 2562
BstNSI RCATGY 3 cut(s) 708, 2435, 2594
BstSCI CCNGG 7 cut(s) 57, 250, 259, 340, 757, 1786, 2560
BstSFI CTRYAG 5 cut(s) 231, 585, 1157, 2262, 2496
BstSLI GKGCMC 3 cut(s) 773, 1977, 2217
BstUI CGCG 1 cut(s) 146
BstV2I GAAGAC 2 cut(s) 56, 1945
BstX2I RGATCY 3 cut(s) 130, 166, 2287
BstYI RGATCY 3 cut(s) 130, 166, 2287
BstZ17I GTATAC 1 cut(s) 2323
Bsu36I CCTNAGG 1 cut(s) 1071
BsuI GTATCC 1 cut(s) 2112
BsuRI GGCC 6 cut(s) 250, 937, 1764, 2215, 2460, 2611
BtgI CCRYGG 3 cut(s) 239, 2461, 2532
BtsCI GGATG 5 cut(s) 598, 799, 895, 1499, 2506
BtsI GCAGTG 2 cut(s) 594, 2144
BtsIMutI CAGTG 8 cut(s) 46, 594, 619, 1315, 1510, 1539, 1578, 2144
BveI ACCTGC 2 cut(s) 1865, 2081
Cac8I GCNNGC 9 cut(s) 507, 645, 706, 1080, 1635, 1965, 2454, 2458, 2592
CaiI CAGNNNCTG 6 cut(s) 341, 764, 1751, 1949, 2120, 2123
CciI TCATGA 1 cut(s) 175
CfoI GCGC 2 cut(s) 148, 1203
Cfr10I RCCGGY 2 cut(s) 317, 957
Cfr13I GGNCC 8 cut(s) 338, 356, 467, 1313, 1877, 2213, 2214, 2558
CseI GACGC 1 cut(s) 678
Csp6I GTAC 5 cut(s) 728, 993, 1610, 1697, 1804
CviQI GTAC 5 cut(s) 728, 993, 1610, 1697, 1804
DpnI GATC 7 cut(s) 56, 74, 132, 168, 723, 1440, 2289
DpnII GATC 7 cut(s) 54, 72, 130, 166, 721, 1438, 2287
EaeI YGGCCR 4 cut(s) 935, 1762, 2458, 2609
Eam1104I CTCTTC 2 cut(s) 1322, 2230
EarI CTCTTC 2 cut(s) 1322, 2230
EciI GGCGGA 1 cut(s) 2025
Ecl136II GAGCTC 1 cut(s) 1332
Eco130I CCWWGG 3 cut(s) 1513, 2461, 2532
Eco147I AGGCCT 1 cut(s) 250
Eco24I GRGCYC 2 cut(s) 1334, 2217
Eco31I GGTCTC 3 cut(s) 134, 586, 1102
Eco47I GGWCC 6 cut(s) 338, 356, 467, 1313, 1877, 2558
Eco53kI GAGCTC 1 cut(s) 1332
Eco57I CTGAAG 1 cut(s) 146
Eco72I CACGTG 1 cut(s) 1667
Eco81I CCTNAGG 1 cut(s) 1071
EcoICRI GAGCTC 1 cut(s) 1332
EcoNI CCTNNNNNAGG 1 cut(s) 2088
EcoO109I RGGNCCY 3 cut(s) 338, 2214, 2558
EcoRII CCWGG 6 cut(s) 57, 250, 340, 757, 1786, 2560
EcoT14I CCWWGG 3 cut(s) 1513, 2461, 2532
EcoT38I GRGCYC 2 cut(s) 1334, 2217
ErhI CCWWGG 3 cut(s) 1513, 2461, 2532
FaqI GGGAC 3 cut(s) 2179, 2509, 2571
FauI CCCGC 1 cut(s) 367
FauNDI CATATG 1 cut(s) 1461
FbaI TGATCA 1 cut(s) 1438
FblI GTMKAC 2 cut(s) 692, 2322
FokI GGATG 5 cut(s) 605, 786, 882, 1486, 2493
FriOI GRGCYC 2 cut(s) 1334, 2217
FspBI CTAG 2 cut(s) 531, 1169
GlaI GCGC 2 cut(s) 147, 1202
GsaI CCCAGC 6 cut(s) 689, 1718, 1971, 2234, 2391, 2541
GsuI CTGGAG 2 cut(s) 643, 1575
HaeIII GGCC 6 cut(s) 250, 937, 1764, 2215, 2460, 2611
HapII CCGG 4 cut(s) 260, 318, 958, 2331
HgaI GACGC 1 cut(s) 678
HhaI GCGC 2 cut(s) 148, 1203
Hin6I GCGC 2 cut(s) 146, 1201
HinP1I GCGC 2 cut(s) 146, 1201
HincII GTYRAC 1 cut(s) 2548
HindII GTYRAC 1 cut(s) 2548
HindIII AAGCTT 2 cut(s) 1270, 1687
HpaII CCGG 4 cut(s) 260, 318, 958, 2331
HphI GGTGA 6 cut(s) 298, 1439, 1443, 1502, 1903, 2320
Hpy166II GTNNAC 5 cut(s) 305, 693, 1975, 2323, 2548
Hpy188III TCNNGA 8 cut(s) 164, 176, 407, 486, 1238, 1316, 1436, 1554
Hpy8I GTNNAC 5 cut(s) 305, 693, 1975, 2323, 2548
HpyAV CCTTC 6 cut(s) 170, 480, 909, 1193, 2339, 2423
HpyCH4III ACNGT 9 cut(s) 41, 243, 454, 499, 516, 1386, 1543, 2009, 2523
HpyCH4IV ACGT 4 cut(s) 193, 781, 1233, 1666
HpySE526I ACGT 4 cut(s) 193, 781, 1233, 1666
HspAI GCGC 2 cut(s) 146, 1201
KpnI GGTACC 1 cut(s) 996
Ksp22I TGATCA 1 cut(s) 1438
Kzo9I GATC 7 cut(s) 54, 72, 130, 166, 721, 1438, 2287
LguI GCTCTTC 2 cut(s) 1322, 2230
LmnI GCTCC 7 cut(s) 391, 662, 701, 759, 1572, 2066, 2113
LweI GCATC 2 cut(s) 304, 583
MaeI CTAG 2 cut(s) 531, 1169
MaeII ACGT 4 cut(s) 193, 781, 1233, 1666
MalI GATC 7 cut(s) 56, 74, 132, 168, 723, 1440, 2289
MbiI CCGCTC 2 cut(s) 362, 1743
MboI GATC 7 cut(s) 54, 72, 130, 166, 721, 1438, 2287
MflI RGATCY 3 cut(s) 130, 166, 2287
MhlI GDGCHC 4 cut(s) 773, 1334, 1977, 2217
MlsI TGGCCA 1 cut(s) 2460
MluCI AATT 8 cut(s) 289, 946, 972, 1175, 1205, 1591, 1617, 1733
MluNI TGGCCA 1 cut(s) 2460
MlyI GAGTC 4 cut(s) 236, 1250, 1636, 2142
MmeI TCCRAC 4 cut(s) 1798, 1904, 1981, 2471
Mox20I TGGCCA 1 cut(s) 2460
MscI TGGCCA 1 cut(s) 2460
MseI TTAA 2 cut(s) 2019, 2393
MslI CAYNNNNRTG 2 cut(s) 2195, 2356
Msp20I TGGCCA 1 cut(s) 2460
MspA1I CMGCKG 5 cut(s) 1967, 2120, 2234, 2495, 2501
MspI CCGG 4 cut(s) 260, 318, 958, 2331
MspR9I CCNGG 7 cut(s) 59, 252, 261, 342, 759, 1788, 2562
Mva1269I GAATGC 2 cut(s) 261, 617
MvaI CCWGG 6 cut(s) 59, 252, 342, 759, 1788, 2562
MvnI CGCG 1 cut(s) 146
NciI CCSGG 1 cut(s) 261
NcoI CCATGG 2 cut(s) 2461, 2532
NdeI CATATG 1 cut(s) 1461
NdeII GATC 7 cut(s) 54, 72, 130, 166, 721, 1438, 2287
NlaIV GGNNCC 9 cut(s) 132, 168, 358, 735, 755, 994, 1077, 2215, 2559
NmeAIII GCCGAG 1 cut(s) 1657
NmuCI GTSAC 5 cut(s) 194, 1107, 1891, 2009, 2434
NspI RCATGY 3 cut(s) 708, 2435, 2594
PaeI GCATGC 2 cut(s) 708, 2594
PagI TCATGA 1 cut(s) 175
PaqCI CACCTGC 1 cut(s) 2081
PceI AGGCCT 1 cut(s) 250
PciSI GCTCTTC 2 cut(s) 1322, 2230
PctI GAATGC 2 cut(s) 261, 617
PfeI GAWTC 6 cut(s) 211, 746, 1133, 1370, 1526, 1655
PflMI CCANNNNNTGG 3 cut(s) 1787, 2056, 2533
PfoI TCCNGGA 2 cut(s) 57, 340
PleI GAGTC 4 cut(s) 235, 1249, 1635, 2141
PmaCI CACGTG 1 cut(s) 1667
PmlI CACGTG 1 cut(s) 1667
PpsI GAGTC 4 cut(s) 235, 1249, 1635, 2141
Ppu21I YACGTR 1 cut(s) 1667
PpuMI RGGWCCY 2 cut(s) 338, 2558
PsiI TTATAA 1 cut(s) 2241
Psp124BI GAGCTC 1 cut(s) 1334
Psp5II RGGWCCY 2 cut(s) 338, 2558
Psp6I CCWGG 6 cut(s) 57, 250, 340, 757, 1786, 2560
PspCI CACGTG 1 cut(s) 1667
PspFI CCCAGC 6 cut(s) 685, 1714, 1967, 2230, 2387, 2537
PspGI CCWGG 6 cut(s) 57, 250, 340, 757, 1786, 2560
PspN4I GGNNCC 9 cut(s) 132, 168, 358, 735, 755, 994, 1077, 2215, 2559
PspOMI GGGCCC 1 cut(s) 2213
PspPI GGNCC 8 cut(s) 338, 356, 467, 1313, 1877, 2213, 2214, 2558
PspPPI RGGWCCY 2 cut(s) 338, 2558
PstI CTGCAG 5 cut(s) 235, 589, 1161, 2266, 2500
PstNI CAGNNNCTG 6 cut(s) 341, 764, 1751, 1949, 2120, 2123
PsuI RGATCY 3 cut(s) 130, 166, 2287
PvuII CAGCTG 5 cut(s) 1967, 2120, 2234, 2495, 2501
RsaI GTAC 5 cut(s) 729, 994, 1611, 1698, 1805
RsaNI GTAC 5 cut(s) 728, 993, 1610, 1697, 1804
RseI CAYNNNNRTG 2 cut(s) 2195, 2356
SacI GAGCTC 1 cut(s) 1334
SapI GCTCTTC 2 cut(s) 1322, 2230
SaqAI TTAA 2 cut(s) 2019, 2393
Sau3AI GATC 7 cut(s) 54, 72, 130, 166, 721, 1438, 2287
Sau96I GGNCC 8 cut(s) 338, 356, 467, 1313, 1877, 2213, 2214, 2558
SchI GAGTC 4 cut(s) 236, 1250, 1636, 2142
ScrFI CCNGG 7 cut(s) 59, 252, 261, 342, 759, 1788, 2562
SduI GDGCHC 4 cut(s) 773, 1334, 1977, 2217
SfaNI GCATC 2 cut(s) 304, 583
SfcI CTRYAG 5 cut(s) 231, 585, 1157, 2262, 2496
SinI GGWCC 6 cut(s) 338, 356, 467, 1313, 1877, 2558
SmiMI CAYNNNNRTG 2 cut(s) 2195, 2356
SmlI CTYRAG 4 cut(s) 113, 1390, 1659, 2085
SmoI CTYRAG 4 cut(s) 113, 1390, 1659, 2085
SpeI ACTAGT 1 cut(s) 530
SphI GCATGC 2 cut(s) 708, 2594
Sse9I AATT 8 cut(s) 289, 946, 972, 1175, 1205, 1591, 1617, 1733
SseBI AGGCCT 1 cut(s) 250
SsiI CCGC 8 cut(s) 144, 360, 921, 1103, 1637, 1741, 1961, 2036
SspI AATATT 1 cut(s) 2054
SspMI CTAG 2 cut(s) 531, 1169
SstI GAGCTC 1 cut(s) 1334
StuI AGGCCT 1 cut(s) 250
StyD4I CCNGG 7 cut(s) 57, 250, 259, 340, 757, 1786, 2560
StyI CCWWGG 3 cut(s) 1513, 2461, 2532
TaaI ACNGT 9 cut(s) 41, 243, 454, 499, 516, 1386, 1543, 2009, 2523
TaiI ACGT 4 cut(s) 196, 784, 1236, 1669
TaqII GACCGA 2 cut(s) 568, 1332
TasI AATT 8 cut(s) 289, 946, 972, 1175, 1205, 1591, 1617, 1733
TatI WGTACW 3 cut(s) 727, 1696, 1803
TauI GCSGC 1 cut(s) 1640
TfiI GAWTC 6 cut(s) 211, 746, 1133, 1370, 1526, 1655
Tru1I TTAA 2 cut(s) 2019, 2393
Tru9I TTAA 2 cut(s) 2019, 2393
TscAI CASTG 8 cut(s) 46, 594, 619, 1315, 1510, 1546, 1578, 2151
TseFI GTSAC 5 cut(s) 194, 1107, 1891, 2009, 2434
Tsp45I GTSAC 5 cut(s) 194, 1107, 1891, 2009, 2434
TspDTI ATGAA 7 cut(s) 164, 285, 891, 1539, 2298, 2340, 2391
TspRI CASTG 8 cut(s) 46, 594, 619, 1315, 1510, 1546, 1578, 2151
Van91I CCANNNNNTGG 3 cut(s) 1787, 2056, 2533
VneI GTGCAC 1 cut(s) 1973
VpaK11BI GGWCC 6 cut(s) 338, 356, 467, 1313, 1877, 2558
XagI CCTNNNNNAGG 1 cut(s) 2088
XapI RAATTY 1 cut(s) 289
XceI RCATGY 3 cut(s) 708, 2435, 2594
XcmI CCANNNNNNNNNTGG 1 cut(s) 2534
XmiI GTMKAC 2 cut(s) 692, 2322
XspI CTAG 2 cut(s) 531, 1169
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.