Rw2G042340

Protein of unknown function (DUF1296)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0007082
Physical Location & Seq
Reverse (-)
68645859 .. 68654036
8178 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G042340.1

Sequence Viewer

Length: 2628 bp
ATGAGTAGTGGTGGTGGGTTTAGGGTTTCAATCCCAAACAGTGTGAGGAAGACGATCCAGGACATCAAAGAGATCACAGGCAATCACAGCGAGGAAGAAATCTATGCAATGCTCAAGGAATGTAACATGGATCCCAACGAGACCGCGCAGAAACTTCTCCTTCAGGATCCATTTCATGAGGTCAAAAGGAAACGTGACAAGAGAAAAGAGAATCTGAACAACAGAGAGTCTGCAGAGCCACGGTGGAGGCCTGGAATGCCGGGTAGGGGGGGTAGGGGTGGTCGGGTGAATTTTTCATCTCGTCCACCACATGATGCCGGTGGTGGCAGGAACTCAGGTCCTGGAACTGAGAATGGACCTGCTCAGGTTGCAGAGAAAGGTGTTGCTCCCTCTTTGCCAACCACTCGTGAGACAAAAACTAAAGAGAGAAGTCTGGTAACAAGCTCTGTACCTGTGATTGTGGGTGGTCCTTCCAATGTGGCTTCGGGAACTACTACTGTTGTGCCTGCCTCACAGTTATCTACTGGAACTAGTGCTGAAATAAGTTTGTCATTGGTTGGGGATAAATCGGGTAGCTCGGTCTCTGCAGTGGATGCAAAAAAAATCCCCACCAGTGCATTCGGAAATGAGGACTTGCACGAGCAGGCTGCACCAAGCTCCAGTAGTAGCTCAGTTTTGCCAAACCCAGCGTCTACATTGGGAGCATGCTTTTCCCCCTCTGATCCTGTACTGGTGCCGTCTAATGATTCACGGCTCCCAGGTTCTGTGGGCACAATCAAACGTGAAGTTGTGAGCCATCATCCTCCTGCAGCTTCTGAGGTTGGCAGCTCTTTAGCACAAGGAAAAACAACAAGCAAAACACAGGGAGTTGGGAAAACTCAGCCCTCTGATTTTTCACATCCTTCATCTACATCAACTCATGGCGGTTCGGTTAGTCGGCCATCATCTAATTATAGTAGCCGGTCGCAACAACTAATTGGCACTCAAAAAGTTGGTACCAACAAGGAGTGGAAACCAAAGCCCATAGTTTCTGCTGTTGTTCAAGGTCAAGCAACAGCTAATGTAGCTGCACCTGAGGTGCCTGCTGATTTGGTAGAGGTCTCTCCGCAGTCACAACCTGTTTCAAGTGTCCTTGATTCGGAAGAAGCAACTTCAAAACTGCAGAAGAAGCTAGAGGAATTACACCTTCCACAGCTTCCACAGCGCAAATTAGTTATTCTTCCAAATCACATACACGTTCCTGAGTCTGAACGAAACAAGTTGAGTTTTGGAAGCTTTGGGGCAACTTTTGGTGTAACGAATAGCTATGTCAGTGGTCCTGAGAGTGAGAAGAGTTCTACCCCTCGGTCTGAAACTTCTCAGGTTATTGAAGAATCTGTGGAGGAACAGTCCTCAAGCAATCAAAATGTGTTGGCAACTGCTGATGAGGGTGATTTTCCTGATCATTCGCAATCACCTACTCATATGCTTGAAAATCTATCATCTGTTGAGGGTGATGTCTCATCCAGTGCAGCCCAAGGACATAATGAATCTAAGCATGACACTGTATTGACTTCTGGAGGTCATCAGCTCCCAGTGGCTAATACTTCTTCGAATTATAGTTTTGGTATCGTACCTCCAATTTTAGGGAGTCAGCTTGCGGCATTTGAAAACTCTGAATCTCAAGCACGTGATATATCTCGGCTTCCAAGCTTTGTAGTACAACAACCCCTTGACCCAGCAAGTTATTATGCCCAATTTTACCGCTCAGGTGCTGATAGTGATGGCCGTCTTTCTCCTTTTCCATCTCCTGGGGTATCAACAAAGTACAATGGCAATGTTGGAGTATTGCCTCCAAGTTCTCAGTCTCCACAAGAGGGTGGGGTTCTTTCAGCAGCAGGTCCAACTCCATTGGTGACACAGGCTTCTGGGCTTGTGCAAAGCTCAATAGCTGTAACTCAGCAGCCAGTTCCTGTCTTCCGACCACCAGCTGGGGTGCACATTCCTCATTATGCTAACTACATTCCTTACAGTCACTACTTTAACCCATTCTATGTTCCGCCTCCTATCCACCAATATTTGGGTAATGGAGCATTTCCTCAACAACCTCAAGCAGGTGGTGTGTATCCAGCTCCACCAGCAGCTGCTGCCACAGGAGTCAAATACTCACTGCCACAATACAAAGCAGGGACTAATACAGGAAACTCAAATCACATGGGAATGGCAAGTGGATATGGGCCCTATGGTTCTTCCCCAGCTGGTTATAATCCCAGTCCTGCCACGACTGCAGGAAACACTACTCCCAATGAAGATCTTAGCACATCTCAGTTCAAGGAGAGTAATGTATACATCACCGGACAGCAGAGTGAAGGTTCATCTGTATGGGTTGCTGCACCTAACCGAGAAATGCCCAGCTTAACAAGTTCATTTTACAACCTTCCTCCACAGGGTCAGCATGTGACTTTCACTCCAACGCAGGCTGGCCATGGTTTCTACCACCCTGCACAAGCAGTATCAGCTGCAGCTGTCCATCCACTTCTGCAACAGTCCCAAACCATGGCTGGGGCAGTTGACATGGTGGGACCTGGGGGAAGTGTTTATCAGCAGCCTCAGCATGCACAACAAATCAACTGGCCGAGCAACTACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1296

Protein Analysis

875

Amino Acids

91.9

Weight (kDa)

6.3

Isoelectric Point (pI)

63.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GIP1_N PF06972 11 - 70 1e-34 GBF-interacting protein 1 N-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2244
AarI CACCTGC 1 cut(s) 2084
Acc36I ACCTGC 3 cut(s) 367, 1868, 2084
Acc65I GGTACC 1 cut(s) 995
AccB1I GGYRCC 3 cut(s) 733, 995, 1078
AccB7I CCANNNNNTGG 4 cut(s) 1790, 1970, 2059, 2536
AccBSI CCGCTC 1 cut(s) 1746
AccI GTMKAC 2 cut(s) 692, 2325
AccII CGCG 1 cut(s) 146
AciI CCGC 6 cut(s) 144, 924, 1106, 1640, 1744, 2039
AclWI GGATC 6 cut(s) 49, 125, 138, 161, 174, 716
AcoI YGGCCR 4 cut(s) 938, 1765, 2461, 2612
AcsI RAATTY 1 cut(s) 289
AcuI CTGAAG 1 cut(s) 146
AcvI CACGTG 1 cut(s) 1670
AfaI GTAC 6 cut(s) 450, 729, 997, 1614, 1701, 1808
AflIII ACRYGT 1 cut(s) 1234
AgsI TTSAA 8 cut(s) 30, 1043, 1125, 1155, 1370, 1472, 1649, 2311
AhlI ACTAGT 1 cut(s) 530
AjnI CCWGG 6 cut(s) 57, 250, 340, 757, 1789, 2563
Alw21I GWGCWC 1 cut(s) 1980
Alw26I GTCTC 6 cut(s) 134, 404, 586, 1105, 1504, 1851
Alw44I GTGCAC 1 cut(s) 1976
AlwI GGATC 6 cut(s) 49, 125, 138, 161, 174, 716
AlwNI CAGNNNCTG 7 cut(s) 341, 764, 815, 1754, 1952, 2123, 2126
AoxI GGCC 6 cut(s) 248, 938, 1765, 2216, 2461, 2612
ApaI GGGCCC 1 cut(s) 2220
ApaLI GTGCAC 1 cut(s) 1976
ApoI RAATTY 1 cut(s) 289
ArsI GACNNNNNNTTYG 2 cut(s) 674, 706
Asp718I GGTACC 1 cut(s) 995
AspLEI GCGC 2 cut(s) 148, 1206
AspS9I GGNCC 8 cut(s) 338, 356, 467, 1316, 1880, 2216, 2217, 2561
AsuC2I CCSGG 1 cut(s) 261
AsuHPI GGTGA 6 cut(s) 298, 1442, 1446, 1505, 1906, 2323
AsuII TTCGAA 1 cut(s) 1592
AvaII GGWCC 6 cut(s) 338, 356, 467, 1316, 1880, 2561
AxyI CCTNAGG 1 cut(s) 1074
BaeGI GKGCMC 3 cut(s) 773, 1980, 2220
BalI TGGCCA 1 cut(s) 2463
BamHI GGATCC 2 cut(s) 130, 166
BanI GGYRCC 3 cut(s) 733, 995, 1078
BanII GRGCYC 1 cut(s) 2220
BauI CACGAG 2 cut(s) 405, 638
BbrPI CACGTG 1 cut(s) 1670
BbsI GAAGAC 2 cut(s) 56, 1948
Bbv12I GWGCWC 1 cut(s) 1980
BbvCI CCTCAGC 1 cut(s) 2589
BccI CCATC 5 cut(s) 804, 949, 1757, 1792, 2517
BceAI ACGGC 3 cut(s) 721, 767, 1752
BcgI CGANNNNNNTGC 2 cut(s) 629, 663
BciT130I CCWGG 6 cut(s) 59, 252, 342, 759, 1791, 2565
BciVI GTATCC 1 cut(s) 2115
BclI TGATCA 1 cut(s) 1441
BcnI CCSGG 1 cut(s) 261
BcoDI GTCTC 6 cut(s) 134, 404, 586, 1105, 1504, 1851
BcuI ACTAGT 1 cut(s) 530
BfaI CTAG 2 cut(s) 531, 1172
BfmI CTRYAG 6 cut(s) 231, 585, 807, 1160, 2265, 2499
BfuAI ACCTGC 3 cut(s) 367, 1868, 2084
BfuI GTATCC 1 cut(s) 2115
BglII AGATCT 1 cut(s) 2290
Bme1390I CCNGG 7 cut(s) 59, 252, 261, 342, 759, 1791, 2565
Bme18I GGWCC 6 cut(s) 338, 356, 467, 1316, 1880, 2561
BmgT120I GGNCC 8 cut(s) 338, 356, 467, 1316, 1880, 2216, 2217, 2561
BmiI GGNNCC 8 cut(s) 132, 168, 735, 755, 997, 1080, 2218, 2562
BmrFI CCNGG 7 cut(s) 59, 252, 261, 342, 759, 1791, 2565
BmrI ACTGGG 2 cut(s) 1568, 2244
BmsI GCATC 2 cut(s) 304, 583
BmuI ACTGGG 2 cut(s) 1568, 2244
BpiI GAAGAC 2 cut(s) 56, 1948
BpmI CTGGAG 2 cut(s) 643, 1578
Bpu10I CCTNAGC 3 cut(s) 363, 1747, 2589
Bpu14I TTCGAA 1 cut(s) 1592
BpuEI CTTGAG 4 cut(s) 98, 1378, 1647, 2073
BpuMI CCSGG 1 cut(s) 261
BsaAI YACGTR 1 cut(s) 1670
BsaI GGTCTC 3 cut(s) 134, 586, 1105
BsaJI CCNNGG 8 cut(s) 239, 757, 1343, 1516, 1790, 2464, 2535, 2564
BsaWI WCCGGW 1 cut(s) 2333
BsaXI ACNNNNNCTCC 4 cut(s) 1373, 1403, 2431, 2461
Bse118I RCCGGY 2 cut(s) 317, 960
Bse1I ACTGG 9 cut(s) 529, 612, 660, 735, 1506, 1574, 1946, 2250, 2615
Bse21I CCTNAGG 1 cut(s) 1074
Bse3DI GCAATG 2 cut(s) 114, 1822
BseBI CCWGG 6 cut(s) 59, 252, 342, 759, 1791, 2565
BseDI CCNNGG 8 cut(s) 239, 757, 1343, 1516, 1790, 2464, 2535, 2564
BseGI GGATG 5 cut(s) 598, 799, 898, 1502, 2509
BseMI GCAATG 2 cut(s) 114, 1822
BseNI ACTGG 9 cut(s) 529, 612, 660, 735, 1506, 1574, 1946, 2250, 2615
BseSI GKGCMC 3 cut(s) 773, 1980, 2220
BseYI CCCAGC 6 cut(s) 685, 1717, 1970, 2233, 2390, 2540
BsgI GTGCAG 5 cut(s) 633, 1053, 1530, 2355, 2466
Bsh1236I CGCG 1 cut(s) 146
Bsh1285I CGRYCG 1 cut(s) 965
BshFI GGCC 6 cut(s) 250, 940, 1767, 2218, 2463, 2614
BshNI GGYRCC 3 cut(s) 733, 995, 1078
BsiEI CGRYCG 1 cut(s) 965
BsiHKAI GWGCWC 1 cut(s) 1980
BsiSI CCGG 4 cut(s) 260, 318, 961, 2334
BslFI GGGAC 3 cut(s) 2182, 2512, 2574
BsmAI GTCTC 6 cut(s) 134, 404, 586, 1105, 1504, 1851
BsmFI GGGAC 3 cut(s) 2182, 2512, 2574
BsmI GAATGC 2 cut(s) 261, 617
BsnI GGCC 6 cut(s) 250, 940, 1767, 2218, 2463, 2614
Bso31I GGTCTC 3 cut(s) 134, 586, 1105
Bsp119I TTCGAA 1 cut(s) 1592
Bsp120I GGGCCC 1 cut(s) 2216
Bsp1286I GDGCHC 3 cut(s) 773, 1980, 2220
Bsp143I GATC 7 cut(s) 54, 72, 130, 166, 721, 1441, 2290
Bsp19I CCATGG 2 cut(s) 2464, 2535
BspACI CCGC 6 cut(s) 144, 924, 1106, 1640, 1744, 2039
BspANI GGCC 6 cut(s) 250, 940, 1767, 2218, 2463, 2614
BspFNI CGCG 1 cut(s) 146
BspHI TCATGA 1 cut(s) 175
BspLI GGNNCC 8 cut(s) 132, 168, 735, 755, 997, 1080, 2218, 2562
BspMAI CTGCAG 6 cut(s) 235, 589, 811, 1164, 2269, 2503
BspMI ACCTGC 3 cut(s) 367, 1868, 2084
BspPI GGATC 6 cut(s) 49, 125, 138, 161, 174, 716
BspT104I TTCGAA 1 cut(s) 1592
BspT107I GGYRCC 3 cut(s) 733, 995, 1078
BspTNI GGTCTC 3 cut(s) 134, 586, 1105
BsrBI CCGCTC 1 cut(s) 1746
BsrDI GCAATG 2 cut(s) 114, 1822
BsrFI RCCGGY 2 cut(s) 317, 960
BsrI ACTGG 9 cut(s) 529, 612, 660, 735, 1506, 1574, 1946, 2250, 2615
BssAI RCCGGY 2 cut(s) 317, 960
BssECI CCNNGG 8 cut(s) 239, 757, 1343, 1516, 1790, 2464, 2535, 2564
BssMI GATC 7 cut(s) 54, 72, 130, 166, 721, 1441, 2290
BssNAI GTATAC 1 cut(s) 2326
BssSI CACGAG 2 cut(s) 405, 638
BssT1I CCWWGG 3 cut(s) 1516, 2464, 2535
Bst1107I GTATAC 1 cut(s) 2326
Bst2BI CACGAG 2 cut(s) 405, 638
Bst2UI CCWGG 6 cut(s) 59, 252, 342, 759, 1791, 2565
Bst4CI ACNGT 8 cut(s) 41, 243, 499, 516, 1389, 1546, 2012, 2526
Bst6I CTCTTC 1 cut(s) 1325
BstAPI GCANNNNNTGC 2 cut(s) 593, 2126
BstBAI YACGTR 1 cut(s) 1670
BstBI TTCGAA 1 cut(s) 1592
BstC8I GCNNGC 8 cut(s) 507, 645, 706, 1083, 1638, 2457, 2461, 2595
BstDSI CCRYGG 3 cut(s) 239, 2464, 2535
BstENI CCTNNNNNAGG 1 cut(s) 2091
BstF5I GGATG 5 cut(s) 598, 799, 898, 1502, 2509
BstFNI CGCG 1 cut(s) 146
BstHHI GCGC 2 cut(s) 148, 1206
BstKTI GATC 7 cut(s) 57, 75, 133, 169, 724, 1444, 2293
BstMAI GTCTC 6 cut(s) 134, 404, 586, 1105, 1504, 1851
BstMBI GATC 7 cut(s) 54, 72, 130, 166, 721, 1441, 2290
BstMCI CGRYCG 1 cut(s) 965
BstNI CCWGG 6 cut(s) 59, 252, 342, 759, 1791, 2565
BstNSI RCATGY 3 cut(s) 708, 2438, 2597
BstSCI CCNGG 7 cut(s) 57, 250, 259, 340, 757, 1789, 2563
BstSFI CTRYAG 6 cut(s) 231, 585, 807, 1160, 2265, 2499
BstSLI GKGCMC 3 cut(s) 773, 1980, 2220
BstUI CGCG 1 cut(s) 146
BstV2I GAAGAC 2 cut(s) 56, 1948
BstX2I RGATCY 3 cut(s) 130, 166, 2290
BstYI RGATCY 3 cut(s) 130, 166, 2290
BstZ17I GTATAC 1 cut(s) 2326
Bsu36I CCTNAGG 1 cut(s) 1074
BsuI GTATCC 1 cut(s) 2115
BsuRI GGCC 6 cut(s) 250, 940, 1767, 2218, 2463, 2614
BtgI CCRYGG 3 cut(s) 239, 2464, 2535
BtsCI GGATG 5 cut(s) 598, 799, 898, 1502, 2509
BtsI GCAGTG 2 cut(s) 594, 2147
BtsIMutI CAGTG 8 cut(s) 46, 594, 619, 1318, 1513, 1542, 1581, 2147
BveI ACCTGC 3 cut(s) 367, 1868, 2084
Cac8I GCNNGC 8 cut(s) 507, 645, 706, 1083, 1638, 2457, 2461, 2595
CaiI CAGNNNCTG 7 cut(s) 341, 764, 815, 1754, 1952, 2123, 2126
CciI TCATGA 1 cut(s) 175
CfoI GCGC 2 cut(s) 148, 1206
Cfr10I RCCGGY 2 cut(s) 317, 960
Cfr13I GGNCC 8 cut(s) 338, 356, 467, 1316, 1880, 2216, 2217, 2561
CseI GACGC 1 cut(s) 678
Csp6I GTAC 6 cut(s) 449, 728, 996, 1613, 1700, 1807
CviQI GTAC 6 cut(s) 449, 728, 996, 1613, 1700, 1807
DpnI GATC 7 cut(s) 56, 74, 132, 168, 723, 1443, 2292
DpnII GATC 7 cut(s) 54, 72, 130, 166, 721, 1441, 2290
EaeI YGGCCR 4 cut(s) 938, 1765, 2461, 2612
Eam1104I CTCTTC 1 cut(s) 1325
EarI CTCTTC 1 cut(s) 1325
EciI GGCGGA 1 cut(s) 2028
Eco130I CCWWGG 3 cut(s) 1516, 2464, 2535
Eco147I AGGCCT 1 cut(s) 250
Eco24I GRGCYC 1 cut(s) 2220
Eco31I GGTCTC 3 cut(s) 134, 586, 1105
Eco47I GGWCC 6 cut(s) 338, 356, 467, 1316, 1880, 2561
Eco57I CTGAAG 1 cut(s) 146
Eco72I CACGTG 1 cut(s) 1670
Eco81I CCTNAGG 1 cut(s) 1074
EcoNI CCTNNNNNAGG 1 cut(s) 2091
EcoO109I RGGNCCY 3 cut(s) 338, 2217, 2561
EcoRII CCWGG 6 cut(s) 57, 250, 340, 757, 1789, 2563
EcoT14I CCWWGG 3 cut(s) 1516, 2464, 2535
EcoT38I GRGCYC 1 cut(s) 2220
ErhI CCWWGG 3 cut(s) 1516, 2464, 2535
FaqI GGGAC 3 cut(s) 2182, 2512, 2574
FauNDI CATATG 1 cut(s) 1464
FbaI TGATCA 1 cut(s) 1441
FblI GTMKAC 2 cut(s) 692, 2325
FokI GGATG 5 cut(s) 605, 786, 885, 1489, 2496
FriOI GRGCYC 1 cut(s) 2220
FspBI CTAG 2 cut(s) 531, 1172
GlaI GCGC 2 cut(s) 147, 1205
GsaI CCCAGC 6 cut(s) 689, 1721, 1974, 2237, 2394, 2544
GsuI CTGGAG 2 cut(s) 643, 1578
HaeIII GGCC 6 cut(s) 250, 940, 1767, 2218, 2463, 2614
HapII CCGG 4 cut(s) 260, 318, 961, 2334
HgaI GACGC 1 cut(s) 678
HhaI GCGC 2 cut(s) 148, 1206
Hin6I GCGC 2 cut(s) 146, 1204
HinP1I GCGC 2 cut(s) 146, 1204
HincII GTYRAC 1 cut(s) 2551
HindII GTYRAC 1 cut(s) 2551
HindIII AAGCTT 2 cut(s) 1273, 1690
HpaII CCGG 4 cut(s) 260, 318, 961, 2334
HphI GGTGA 6 cut(s) 298, 1442, 1446, 1505, 1906, 2323
Hpy166II GTNNAC 5 cut(s) 305, 693, 1978, 2326, 2551
Hpy188III TCNNGA 8 cut(s) 164, 176, 407, 486, 1241, 1319, 1439, 1557
Hpy8I GTNNAC 5 cut(s) 305, 693, 1978, 2326, 2551
HpyAV CCTTC 6 cut(s) 170, 480, 912, 1196, 2342, 2426
HpyCH4III ACNGT 8 cut(s) 41, 243, 499, 516, 1389, 1546, 2012, 2526
HpyCH4IV ACGT 4 cut(s) 193, 781, 1236, 1669
HpySE526I ACGT 4 cut(s) 193, 781, 1236, 1669
HspAI GCGC 2 cut(s) 146, 1204
KpnI GGTACC 1 cut(s) 999
Ksp22I TGATCA 1 cut(s) 1441
Kzo9I GATC 7 cut(s) 54, 72, 130, 166, 721, 1441, 2290
LmnI GCTCC 7 cut(s) 391, 662, 701, 759, 1575, 2069, 2116
LweI GCATC 2 cut(s) 304, 583
MaeI CTAG 2 cut(s) 531, 1172
MaeII ACGT 4 cut(s) 193, 781, 1236, 1669
MaeIII GTNAC 9 cut(s) 122, 194, 436, 1110, 1294, 1894, 1933, 2012, 2437
MalI GATC 7 cut(s) 56, 74, 132, 168, 723, 1443, 2292
MbiI CCGCTC 1 cut(s) 1746
MboI GATC 7 cut(s) 54, 72, 130, 166, 721, 1441, 2290
MflI RGATCY 3 cut(s) 130, 166, 2290
MhlI GDGCHC 3 cut(s) 773, 1980, 2220
MlsI TGGCCA 1 cut(s) 2463
MluCI AATT 8 cut(s) 289, 949, 975, 1178, 1208, 1594, 1620, 1736
MluNI TGGCCA 1 cut(s) 2463
MlyI GAGTC 4 cut(s) 236, 1253, 1639, 2145
MmeI TCCRAC 4 cut(s) 1801, 1907, 1984, 2474
Mox20I TGGCCA 1 cut(s) 2463
MscI TGGCCA 1 cut(s) 2463
MseI TTAA 2 cut(s) 2022, 2396
MslI CAYNNNNRTG 2 cut(s) 2198, 2359
Msp20I TGGCCA 1 cut(s) 2463
MspA1I CMGCKG 5 cut(s) 1970, 2123, 2237, 2498, 2504
MspI CCGG 4 cut(s) 260, 318, 961, 2334
MspR9I CCNGG 7 cut(s) 59, 252, 261, 342, 759, 1791, 2565
Mva1269I GAATGC 2 cut(s) 261, 617
MvaI CCWGG 6 cut(s) 59, 252, 342, 759, 1791, 2565
MvnI CGCG 1 cut(s) 146
NciI CCSGG 1 cut(s) 261
NcoI CCATGG 2 cut(s) 2464, 2535
NdeI CATATG 1 cut(s) 1464
NdeII GATC 7 cut(s) 54, 72, 130, 166, 721, 1441, 2290
NlaIV GGNNCC 8 cut(s) 132, 168, 735, 755, 997, 1080, 2218, 2562
NmeAIII GCCGAG 1 cut(s) 1660
NmuCI GTSAC 5 cut(s) 194, 1110, 1894, 2012, 2437
NspI RCATGY 3 cut(s) 708, 2438, 2597
NspV TTCGAA 1 cut(s) 1592
PaeI GCATGC 2 cut(s) 708, 2597
PagI TCATGA 1 cut(s) 175
PaqCI CACCTGC 1 cut(s) 2084
PceI AGGCCT 1 cut(s) 250
PctI GAATGC 2 cut(s) 261, 617
PfeI GAWTC 6 cut(s) 211, 746, 1136, 1373, 1529, 1658
PflMI CCANNNNNTGG 4 cut(s) 1790, 1970, 2059, 2536
PfoI TCCNGGA 2 cut(s) 57, 340
PleI GAGTC 4 cut(s) 235, 1252, 1638, 2144
PmaCI CACGTG 1 cut(s) 1670
PmlI CACGTG 1 cut(s) 1670
PpsI GAGTC 4 cut(s) 235, 1252, 1638, 2144
Ppu21I YACGTR 1 cut(s) 1670
PpuMI RGGWCCY 2 cut(s) 338, 2561
PsiI TTATAA 1 cut(s) 2244
Psp5II RGGWCCY 2 cut(s) 338, 2561
Psp6I CCWGG 6 cut(s) 57, 250, 340, 757, 1789, 2563
PspCI CACGTG 1 cut(s) 1670
PspFI CCCAGC 6 cut(s) 685, 1717, 1970, 2233, 2390, 2540
PspGI CCWGG 6 cut(s) 57, 250, 340, 757, 1789, 2563
PspN4I GGNNCC 8 cut(s) 132, 168, 735, 755, 997, 1080, 2218, 2562
PspOMI GGGCCC 1 cut(s) 2216
PspPI GGNCC 8 cut(s) 338, 356, 467, 1316, 1880, 2216, 2217, 2561
PspPPI RGGWCCY 2 cut(s) 338, 2561
PstI CTGCAG 6 cut(s) 235, 589, 811, 1164, 2269, 2503
PstNI CAGNNNCTG 7 cut(s) 341, 764, 815, 1754, 1952, 2123, 2126
PsuI RGATCY 3 cut(s) 130, 166, 2290
PvuII CAGCTG 5 cut(s) 1970, 2123, 2237, 2498, 2504
RsaI GTAC 6 cut(s) 450, 729, 997, 1614, 1701, 1808
RsaNI GTAC 6 cut(s) 449, 728, 996, 1613, 1700, 1807
RseI CAYNNNNRTG 2 cut(s) 2198, 2359
SaqAI TTAA 2 cut(s) 2022, 2396
Sau3AI GATC 7 cut(s) 54, 72, 130, 166, 721, 1441, 2290
Sau96I GGNCC 8 cut(s) 338, 356, 467, 1316, 1880, 2216, 2217, 2561
SchI GAGTC 4 cut(s) 236, 1253, 1639, 2145
ScrFI CCNGG 7 cut(s) 59, 252, 261, 342, 759, 1791, 2565
SduI GDGCHC 3 cut(s) 773, 1980, 2220
SfaNI GCATC 2 cut(s) 304, 583
SfcI CTRYAG 6 cut(s) 231, 585, 807, 1160, 2265, 2499
SfuI TTCGAA 1 cut(s) 1592
SinI GGWCC 6 cut(s) 338, 356, 467, 1316, 1880, 2561
SmiMI CAYNNNNRTG 2 cut(s) 2198, 2359
SmlI CTYRAG 4 cut(s) 113, 1393, 1662, 2088
SmoI CTYRAG 4 cut(s) 113, 1393, 1662, 2088
SpeI ACTAGT 1 cut(s) 530
SphI GCATGC 2 cut(s) 708, 2597
Sse9I AATT 8 cut(s) 289, 949, 975, 1178, 1208, 1594, 1620, 1736
SseBI AGGCCT 1 cut(s) 250
SsiI CCGC 6 cut(s) 144, 924, 1106, 1640, 1744, 2039
SspI AATATT 1 cut(s) 2057
SspMI CTAG 2 cut(s) 531, 1172
StuI AGGCCT 1 cut(s) 250
StyD4I CCNGG 7 cut(s) 57, 250, 259, 340, 757, 1789, 2563
StyI CCWWGG 3 cut(s) 1516, 2464, 2535
TaaI ACNGT 8 cut(s) 41, 243, 499, 516, 1389, 1546, 2012, 2526
TaiI ACGT 4 cut(s) 196, 784, 1239, 1672
TaqI TCGA 1 cut(s) 1592
TaqII GACCGA 2 cut(s) 568, 1335
TasI AATT 8 cut(s) 289, 949, 975, 1178, 1208, 1594, 1620, 1736
TatI WGTACW 3 cut(s) 727, 1699, 1806
TauI GCSGC 1 cut(s) 1643
TfiI GAWTC 6 cut(s) 211, 746, 1136, 1373, 1529, 1658
Tru1I TTAA 2 cut(s) 2022, 2396
Tru9I TTAA 2 cut(s) 2022, 2396
TscAI CASTG 8 cut(s) 46, 594, 619, 1318, 1513, 1549, 1581, 2154
TseFI GTSAC 5 cut(s) 194, 1110, 1894, 2012, 2437
Tsp45I GTSAC 5 cut(s) 194, 1110, 1894, 2012, 2437
TspDTI ATGAA 7 cut(s) 164, 285, 894, 1542, 2301, 2343, 2394
TspRI CASTG 8 cut(s) 46, 594, 619, 1318, 1513, 1549, 1581, 2154
Van91I CCANNNNNTGG 4 cut(s) 1790, 1970, 2059, 2536
VneI GTGCAC 1 cut(s) 1976
VpaK11BI GGWCC 6 cut(s) 338, 356, 467, 1316, 1880, 2561
XagI CCTNNNNNAGG 1 cut(s) 2091
XapI RAATTY 1 cut(s) 289
XceI RCATGY 3 cut(s) 708, 2438, 2597
XcmI CCANNNNNNNNNTGG 1 cut(s) 2537
XmiI GTMKAC 2 cut(s) 692, 2325
XspI CTAG 2 cut(s) 531, 1172
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.