Rorug02G0449100

Protein of unknown function (DUF1296)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0007082
Physical Location & Seq
Reverse (-)
57608811 .. 57610340
1530 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0449100.1

Sequence Viewer

Length: 1098 bp
ATGTCGACTTCACTGTTTCAAGTATGTATGATCTTTTCTTTTTGTGCAAGTCTTCTTACTCCAATCAGCTGCCATGACAACTCAAGGCTTCACAAGAAACACGCAGCCTTGTTCGTCTTTGGTGATTCGCAGTTTGATGTCGGAAATAATAACTACTTGAACACTTCCACTGAACTTCAAGCAAATTTCTGGCCATATGGGGAAACATTCTTCAAGCAACCCACTGGTAGATTTTCGGATGGACGTCTAATCCCTGATTTTATTGCTGAGTATGCAAAGTTGCCACTTATTCCACCATATTTACGACGTGGGTTCAAAAGCTATACTTATGGGGTCAACTTCGCATCTGCCGGGGCTGGTGCTCTAGCTGAATCTTATCAAGGATTTGTCATAGACCTTAAAACTCAACTGGGTTATTTCAAGAAAGTGGAGAAGCAGTTGAGGCACAAACTAGGCCATGCAGAAGCCTACACATTGCTGTCAGAAGCTGTTTACTTAATTGGCATTGGAGCCAATGACTATTCTTATGCACTCGGCACAAATTCCAGTTTGTTTGAGTCCTCACCTGAAGAATTTGTTGGCCTGGTGATAGGAAACCTGACAACTGTGATCAAGGAAATATACAAGAAAGGAGGAAGGAAATTTGGGTTTACAAGCATGGCTCCTCTGGGTTGTGTACCAAGCATGAGAGCAATGAAACCAGGAAACACAGGCACATGTGTGGAAGAAGTGAATGCGGTAGCGAAACTACACAACAGAGTACTTGATAAAGTCCTCCTAAAGCTTAAAGGACATCTCCCAGGATTCAAGTATTCATATCCAAATGTATACCCTCACATAGACGAAATATTTAACAATGCGTCTAAACATGGTTTCAAGGAGGGAAAGACGGCATGTTGCGGATCTGGTCCATACAGAGGAATTGGGAGTTGTGGAGGGAAGCGAGGCGTGACAGACAATGTTACTGAATATGTGTTTTTCGAATCCAATCATCCCACAGAAAGGTTTTACCAGAAAGTTTCCAAGTTATGGTGGAGCCATACCCCTAAACAGACTGAGACTTACATTGGTCTGAAAGAGCTATTTGAAGTTTATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1296

Protein Analysis

365

Amino Acids

40.75

Weight (kDa)

8.97

Isoelectric Point (pI)

28.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 37 - 343 9e-30 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 247
AccB7I CCANNNNNTGG 1 cut(s) 1029
AccI GTMKAC 2 cut(s) 5, 828
AciI CCGC 2 cut(s) 737, 900
AclWI GGATC 1 cut(s) 910
AcoI YGGCCR 1 cut(s) 191
AcsI RAATTY 4 cut(s) 184, 541, 572, 641
AcuI CTGAAG 1 cut(s) 588
AcyI GRCGYC 1 cut(s) 244
AfaI GTAC 2 cut(s) 678, 762
AfiI CCNNNNNNNGG 3 cut(s) 917, 1002, 1029
AflIII ACRYGT 1 cut(s) 716
AgsI TTSAA 9 cut(s) 20, 160, 179, 214, 316, 421, 808, 877, 1088
AjiI CACGTC 1 cut(s) 308
AjnI CCWGG 3 cut(s) 582, 700, 799
AjuI GAANNNNNNNTTGG 2 cut(s) 561, 593
AleI CACNNNNGTG 1 cut(s) 719
AluBI AGCT 6 cut(s) 69, 321, 368, 488, 784, 1081
AluI AGCT 6 cut(s) 69, 321, 368, 488, 784, 1081
Alw21I GWGCWC 1 cut(s) 364
Alw26I GTCTC 1 cut(s) 1052
AlwI GGATC 1 cut(s) 910
AlwNI CAGNNNCTG 1 cut(s) 488
AoxI GGCC 3 cut(s) 191, 454, 580
ApeKI GCWGC 2 cut(s) 69, 104
ApoI RAATTY 4 cut(s) 184, 541, 572, 641
AspS9I GGNCC 1 cut(s) 908
AsuC2I CCSGG 1 cut(s) 352
AsuHPI GGTGA 3 cut(s) 134, 555, 598
AsuII TTCGAA 1 cut(s) 981
AvaII GGWCC 1 cut(s) 908
BalI TGGCCA 1 cut(s) 193
BbsI GAAGAC 1 cut(s) 44
Bbv12I GWGCWC 1 cut(s) 364
BbvI GCAGC 2 cut(s) 56, 116
BccI CCATC 1 cut(s) 233
BceAI ACGGC 1 cut(s) 906
BciT130I CCWGG 3 cut(s) 584, 702, 801
BclI TGATCA 1 cut(s) 609
BcnI CCSGG 1 cut(s) 352
BcoDI GTCTC 1 cut(s) 1052
BfaI CTAG 2 cut(s) 365, 452
BisI GCNGC 2 cut(s) 70, 105
BlsI GCNGC 2 cut(s) 71, 106
BmcAI AGTACT 1 cut(s) 762
Bme1390I CCNGG 4 cut(s) 352, 584, 702, 801
Bme18I GGWCC 1 cut(s) 908
BmgBI CACGTC 1 cut(s) 308
BmgT120I GGNCC 1 cut(s) 908
BmiI GGNNCC 3 cut(s) 511, 663, 1037
BmrFI CCNGG 4 cut(s) 352, 584, 702, 801
BmrI ACTGGG 1 cut(s) 419
BmsI GCATC 1 cut(s) 353
BmuI ACTGGG 1 cut(s) 419
BpiI GAAGAC 1 cut(s) 44
Bpu14I TTCGAA 1 cut(s) 981
BpuEI CTTGAG 1 cut(s) 67
BpuMI CCSGG 1 cut(s) 352
BsaHI GRCGYC 1 cut(s) 244
BsaJI CCNNGG 2 cut(s) 351, 799
BsaXI ACNNNNNCTCC 2 cut(s) 422, 452
Bsc4I CCNNNNNNNGG 3 cut(s) 917, 1002, 1029
Bse1I ACTGG 3 cut(s) 229, 414, 546
Bse3DI GCAATG 2 cut(s) 473, 699
BseBI CCWGG 3 cut(s) 584, 702, 801
BseDI CCNNGG 2 cut(s) 351, 799
BseGI GGATG 2 cut(s) 244, 991
BseLI CCNNNNNNNGG 3 cut(s) 917, 1002, 1029
BseMI GCAATG 2 cut(s) 473, 699
BseMII CTCAG 2 cut(s) 258, 1047
BseNI ACTGG 3 cut(s) 229, 414, 546
BseRI GAGGAG 1 cut(s) 654
BseXI GCAGC 2 cut(s) 56, 116
BshFI GGCC 3 cut(s) 193, 456, 582
BsiHKAI GWGCWC 1 cut(s) 364
BsiSI CCGG 1 cut(s) 351
BslI CCNNNNNNNGG 3 cut(s) 917, 1002, 1029
BsmAI GTCTC 1 cut(s) 1052
BsmI GAATGC 1 cut(s) 739
BsnI GGCC 3 cut(s) 193, 456, 582
Bsp119I TTCGAA 1 cut(s) 981
Bsp1286I GDGCHC 1 cut(s) 364
Bsp143I GATC 3 cut(s) 30, 609, 902
BspACI CCGC 2 cut(s) 737, 900
BspANI GGCC 3 cut(s) 193, 456, 582
BspCNI CTCAG 2 cut(s) 259, 1048
BspLI GGNNCC 3 cut(s) 511, 663, 1037
BspPI GGATC 1 cut(s) 910
BspT104I TTCGAA 1 cut(s) 981
BsrDI GCAATG 2 cut(s) 473, 699
BsrI ACTGG 3 cut(s) 229, 414, 546
BssECI CCNNGG 2 cut(s) 351, 799
BssMI GATC 3 cut(s) 30, 609, 902
BssNAI GTATAC 1 cut(s) 829
BssNI GRCGYC 1 cut(s) 244
Bst1107I GTATAC 1 cut(s) 829
Bst2UI CCWGG 3 cut(s) 584, 702, 801
Bst4CI ACNGT 2 cut(s) 15, 607
BstACI GRCGYC 1 cut(s) 244
BstBI TTCGAA 1 cut(s) 981
BstDEI CTNAG 2 cut(s) 267, 1056
BstF5I GGATG 2 cut(s) 244, 991
BstKTI GATC 3 cut(s) 33, 612, 905
BstMAI GTCTC 1 cut(s) 1052
BstMBI GATC 3 cut(s) 30, 609, 902
BstMWI GCNNNNNNNGC 2 cut(s) 272, 442
BstNI CCWGG 3 cut(s) 584, 702, 801
BstNSI RCATGY 2 cut(s) 720, 897
BstSCI CCNGG 4 cut(s) 350, 582, 700, 799
BstV1I GCAGC 2 cut(s) 56, 116
BstV2I GAAGAC 1 cut(s) 44
BstX2I RGATCY 1 cut(s) 902
BstYI RGATCY 1 cut(s) 902
BstZ17I GTATAC 1 cut(s) 829
BsuRI GGCC 3 cut(s) 193, 456, 582
BtrI CACGTC 1 cut(s) 308
BtsCI GGATG 2 cut(s) 244, 991
BtsIMutI CAGTG 3 cut(s) 11, 168, 222
CaiI CAGNNNCTG 1 cut(s) 488
Cfr13I GGNCC 1 cut(s) 908
CseI GACGC 1 cut(s) 849
Csp6I GTAC 2 cut(s) 677, 761
CviAII CATG 7 cut(s) 74, 458, 658, 685, 717, 869, 894
CviQI GTAC 2 cut(s) 677, 761
DdeI CTNAG 2 cut(s) 267, 1056
DpnI GATC 3 cut(s) 32, 611, 904
DpnII GATC 3 cut(s) 30, 609, 902
EaeI YGGCCR 1 cut(s) 191
Eco47I GGWCC 1 cut(s) 908
Eco57I CTGAAG 1 cut(s) 588
EcoRII CCWGG 3 cut(s) 582, 700, 799
FaeI CATG 7 cut(s) 77, 461, 661, 688, 720, 872, 897
FalI AAGNNNNNCTT 2 cut(s) 310, 342
FatI CATG 7 cut(s) 73, 457, 657, 684, 716, 868, 893
FauNDI CATATG 1 cut(s) 196
FbaI TGATCA 1 cut(s) 609
FblI GTMKAC 2 cut(s) 5, 828
Fnu4HI GCNGC 2 cut(s) 70, 105
FokI GGATG 2 cut(s) 251, 978
Fsp4HI GCNGC 2 cut(s) 70, 105
FspBI CTAG 2 cut(s) 365, 452
GluI GCNGC 2 cut(s) 70, 105
HaeIII GGCC 3 cut(s) 193, 456, 582
HapII CCGG 1 cut(s) 351
HgaI GACGC 1 cut(s) 849
Hin1I GRCGYC 1 cut(s) 244
Hin1II CATG 7 cut(s) 77, 461, 661, 688, 720, 872, 897
HincII GTYRAC 2 cut(s) 6, 337
HindII GTYRAC 2 cut(s) 6, 337
HindIII AAGCTT 1 cut(s) 782
HinfI GANTC 5 cut(s) 125, 371, 557, 804, 983
HpaII CCGG 1 cut(s) 351
HphI GGTGA 3 cut(s) 134, 555, 598
Hpy166II GTNNAC 6 cut(s) 6, 337, 493, 651, 677, 829
Hpy188I TCNGA 4 cut(s) 143, 238, 484, 1074
Hpy188III TCNNGA 1 cut(s) 421
Hpy8I GTNNAC 6 cut(s) 6, 337, 493, 651, 677, 829
Hpy99I CGWCG 1 cut(s) 309
HpyAV CCTTC 1 cut(s) 630
HpyCH4III ACNGT 2 cut(s) 15, 607
HpyCH4IV ACGT 2 cut(s) 244, 307
HpyCH4V TGCA 4 cut(s) 47, 275, 461, 530
HpyF10VI GCNNNNNNNGC 2 cut(s) 272, 442
HpyF3I CTNAG 2 cut(s) 267, 1056
HpySE526I ACGT 2 cut(s) 244, 307
Hsp92I GRCGYC 1 cut(s) 244
Hsp92II CATG 7 cut(s) 77, 461, 661, 688, 720, 872, 897
Ksp22I TGATCA 1 cut(s) 609
Kzo9I GATC 3 cut(s) 30, 609, 902
LmnI GCTCC 3 cut(s) 509, 667, 1035
Lsp1109I GCAGC 2 cut(s) 56, 116
LweI GCATC 1 cut(s) 353
MaeI CTAG 2 cut(s) 365, 452
MaeII ACGT 2 cut(s) 244, 307
MaeIII GTNAC 2 cut(s) 949, 961
MalI GATC 3 cut(s) 32, 611, 904
MboI GATC 3 cut(s) 30, 609, 902
MboII GAAGA 4 cut(s) 44, 202, 581, 737
MflI RGATCY 1 cut(s) 902
MhlI GDGCHC 1 cut(s) 364
MlsI TGGCCA 1 cut(s) 193
MluCI AATT 6 cut(s) 184, 498, 541, 572, 641, 921
MluNI TGGCCA 1 cut(s) 193
MlyI GAGTC 1 cut(s) 566
MmeI TCCRAC 1 cut(s) 121
Mox20I TGGCCA 1 cut(s) 193
MscI TGGCCA 1 cut(s) 193
MseI TTAA 4 cut(s) 399, 497, 786, 852
MslI CAYNNNNRTG 1 cut(s) 719
Msp20I TGGCCA 1 cut(s) 193
MspA1I CMGCKG 1 cut(s) 69
MspI CCGG 1 cut(s) 351
MspR9I CCNGG 4 cut(s) 352, 584, 702, 801
Mva1269I GAATGC 1 cut(s) 739
MvaI CCWGG 3 cut(s) 584, 702, 801
MwoI GCNNNNNNNGC 2 cut(s) 272, 442
NciI CCSGG 1 cut(s) 352
NdeI CATATG 1 cut(s) 196
NdeII GATC 3 cut(s) 30, 609, 902
NlaIII CATG 7 cut(s) 77, 461, 661, 688, 720, 872, 897
NlaIV GGNNCC 3 cut(s) 511, 663, 1037
NmeAIII GCCGAG 1 cut(s) 513
NmuCI GTSAC 1 cut(s) 949
NspI RCATGY 2 cut(s) 720, 897
NspV TTCGAA 1 cut(s) 981
OliI CACNNNNGTG 1 cut(s) 719
PciI ACATGT 1 cut(s) 716
PctI GAATGC 1 cut(s) 739
PfeI GAWTC 4 cut(s) 125, 371, 804, 983
PflMI CCANNNNNTGG 1 cut(s) 1029
PkrI GCNGC 2 cut(s) 71, 106
PleI GAGTC 1 cut(s) 565
PpsI GAGTC 1 cut(s) 565
PscI ACATGT 1 cut(s) 716
Psp6I CCWGG 3 cut(s) 582, 700, 799
PspGI CCWGG 3 cut(s) 582, 700, 799
PspN4I GGNNCC 3 cut(s) 511, 663, 1037
PspPI GGNCC 1 cut(s) 908
PstNI CAGNNNCTG 1 cut(s) 488
PsuI RGATCY 1 cut(s) 902
PvuII CAGCTG 1 cut(s) 69
RsaI GTAC 2 cut(s) 678, 762
RsaNI GTAC 2 cut(s) 677, 761
RseI CAYNNNNRTG 1 cut(s) 719
SalI GTCGAC 1 cut(s) 4
SaqAI TTAA 4 cut(s) 399, 497, 786, 852
SatI GCNGC 2 cut(s) 70, 105
Sau3AI GATC 3 cut(s) 30, 609, 902
Sau96I GGNCC 1 cut(s) 908
ScaI AGTACT 1 cut(s) 762
SchI GAGTC 1 cut(s) 566
ScrFI CCNGG 4 cut(s) 352, 584, 702, 801
SduI GDGCHC 1 cut(s) 364
SfaNI GCATC 1 cut(s) 353
SfuI TTCGAA 1 cut(s) 981
SinI GGWCC 1 cut(s) 908
SmiMI CAYNNNNRTG 1 cut(s) 719
SmlI CTYRAG 1 cut(s) 82
SmoI CTYRAG 1 cut(s) 82
Sse9I AATT 6 cut(s) 184, 498, 541, 572, 641, 921
SsiI CCGC 2 cut(s) 737, 900
SspI AATATT 1 cut(s) 849
SspMI CTAG 2 cut(s) 365, 452
StyD4I CCNGG 4 cut(s) 350, 582, 700, 799
TaaI ACNGT 2 cut(s) 15, 607
TaiI ACGT 2 cut(s) 247, 310
TaqI TCGA 2 cut(s) 5, 981
TasI AATT 6 cut(s) 184, 498, 541, 572, 641, 921
TatI WGTACW 1 cut(s) 760
TfiI GAWTC 4 cut(s) 125, 371, 804, 983
Tru1I TTAA 4 cut(s) 399, 497, 786, 852
Tru9I TTAA 4 cut(s) 399, 497, 786, 852
TscAI CASTG 3 cut(s) 18, 175, 229
TseFI GTSAC 1 cut(s) 949
TseI GCWGC 2 cut(s) 69, 104
Tsp45I GTSAC 1 cut(s) 949
TspDTI ATGAA 2 cut(s) 710, 804
TspRI CASTG 3 cut(s) 18, 175, 229
Van91I CCANNNNNTGG 1 cut(s) 1029
VpaK11BI GGWCC 1 cut(s) 908
XapI RAATTY 4 cut(s) 184, 541, 572, 641
XceI RCATGY 2 cut(s) 720, 897
XmiI GTMKAC 2 cut(s) 5, 828
XspI CTAG 2 cut(s) 365, 452
ZraI GACGTC 1 cut(s) 245
ZrmI AGTACT 1 cut(s) 762
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.